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immunocytochemical demonstration of visual pigments in the degenerate retinal and pineal photoreceptors of the blind cave salamander (proteus anguinus).visual pigments in the regressed eye and pineal of the depigmented neotenic urodele, the blind cave salamander (proteus anguinus anguinus), were studied by immunocytochemistry with anti-opsin antibodies. the study included light- and electron-microscopic investigations of both the eye and the pineal organ. a comparison was made with the black pigmented subspecies proteus anguinus parkelj (black proteus), which has a normal eye structure. in the retina of the black proteus, we found principal rod ...200111236001
bacterial protease ecp32 specifically hydrolyzing actin and its effect on cytoskeleton in vivo.a procedure for isolation of bacterial protease ecp32 yielding 100 microg of the enzyme from 10 liters of the escherichia coli strain a2 liquid culture has been developed. the procedure includes chromatography, ultrafiltration, and page under non-denaturing conditions. the purified preparation contained about 80% ecp32 and did not exhibit atpase activity. polyclonal ecp32-specific antibodies have been produced, and a two-stage procedure for the isolation of protease ecp32 involving affinity chro ...200111240398
lactobacillus plantarum reduces infection of pancreatic necrosis in experimental acute pancreatitis.infection is the commonest cause of death in acute pancreatitis. early reduction of commensal flora (particularly lactobacillus species) and, at the same time, overgrowth of enterobacteriaceae, especially escherichia coli, have recently been described during acute pancreatitis. lactobacillus plantarum has been shown to be effective in reducing the egress of endotoxin and microbial translocation in several experimental models such as chemically induced hepatitis and ulcerative colitis.200111244259
chronic osteomyelitis: clarification of nuclear medicine findings by fusion scans. 200111245118
[the role of urinary tract infections in burn patients].patients with burns are predisposed to a variety of infectious complications, urinary tract infections inclusive. it is the aim of the study to assay the incidence rate, etiology and antibacterial sensitivity of the leading pathogens causing bacteriuria in burnt patients, and compare them with the respective characteristics of the pathogens causing wound infection in the same contingent of patients, and urinary tract infection in uro-nephrological patients. over a five-year period, a total of 24 ...199811247066
investigation on the nutritive value and microbiological quality of wild quail carcasses.quail meats have many advantages and superiority one the other species of poultry. this study was planned to throw plenty of light on gross chemical composition, lipid fractions, fatty acids composition, amino acids composition, of thigh and breast of male and female wild quail meat as well as the microbiological quality. the mean values of moisture, protein, fat, ash and energy contents ranged from 60.1 to 69.2%, 55.0 to 68.8%, 28.8 to 42.1%, 2.40 to 3.63% and 696 to 1000 kj, respectively. seve ...200111253642
new tem variant (tem-92) produced by proteus mirabilis and providencia stuartii isolates.the sequences of the bla(tem) genes encoding tem-92 in proteus mirabilis and providencia stuartii isolates were determined and were found to be identical. except for positions 218 (lys-6) and 512 (lys-104), the nucleotide sequence of bla(tem-92) was identical to that of bla(tem-20), including the sequence of the promoter region harboring a 135-bp deletion combined with a g-162-->t substitution. the deduced amino acid sequence of tem-92 differed from that of tem-52 by the presence of a substituti ...200111257046
epigenetic regulation of carnitine metabolising enzymes in proteus sp. under aerobic conditions.proteus sp. is able to catalyse the reversible transformation of crotonobetaine into l(-)-carnitine during aerobic growth. contrary to other enterobacteriaceae no reduction of crotonobetaine into gamma-butyrobetaine could be detected in the culture supernatants. activities of l(-)-carnitine dehydratase, carnitine racemasing system and crotonobetaine reductase could be determined enzymatically in cell-free extracts of proteus sp. small amounts of gamma-butyrobetaine were found in cell-free extrac ...200111257539
interaction of bladder glycoprotein gp51 with uropathogenic bacteria.a major component of bladder surface mucin is a glycoprotein gp51 (molecular weight 51 kd.). gp51, which has previously been isolated from rabbit mucosa, appears to function as part of the defense mechanism in an in vivo infection model. gp51 coats the epithelium and is secreted into the urine, as detected by immunohistochemical testing and enzyme-linked immunosorbent assay (elisa). increased urinary gp51 occurs during urinary tract infection. to elucidate the role of gp51 as a component of the ...200111257713
bladder washouts in the management of long-term catheters.it has been estimated that 40-50% of patients with long-term catheters can suffer with catheter blockage. this not only causes distress to the patient but also increases the demands on community nurses' time and resources. infection with bacteria such as proteus causes the urine to become alkaline. crystalline deposits can then form inside the catheter lumen which causes blockage. nurses often manage blocked catheters with the use of bladder 'washouts' or bladder instillations. however, the lite ...200011261025
a new bio-active flavonol glycoside from the stems of butea superba roxb.a new bio-active flavonol glycoside was isolated from the stems of butea superba roxb, and its structure was determined by spectral analysis and chemical degradations as 3,5,7,3',4'-pentahydroxy-8-methoxy-flavonol-3-o-beta-d-xylopyranosyl(1-->2) -alpha-l-rhamnopyranoside. the compound 1 showed antimicrobial activity against plant pathogenic fungi trich viride, asprgillus fumigatus, a. niger, a. terreus, penicillium expansum, helmitnhosporium oryzae, botxitis cinerea, rhizopus oligosporus, r. chi ...199811261215
comparison of the pr mutant with the wild-type strain of proteus mirabilis brings insight into peroxide resistance factors and regulation of catalase expression.the peroxide resistant mutant (pr) of proteus mirabilis was characterized by an increased constitutive catalase activity concomitant with a large production of specific mrna. survival toward hydrogen peroxide during exponential phase was increased by h2o2 pretreatment in the wild type but not in the mutant, although the catalase of both strains was not inducible under these conditions. in the mutant, besides catalase, over-produced proteins comprised two different alkyl hydroperoxide reductase s ...200111261492
differentiation of bacterial autolysins by zymogram analysis.the use of zymograms in which the bacterial cell wall heteropolymer peptidoglycan is incorporated into the resolving gel of sds-page has led to the identification of various sds stable peptidoglycan hydrolases (autolysins). to examine the specificity of autolysins with respect to o-acetylated peptidoglycan, a discontinuous sds-page system has been developed that operates under neutral conditions. [bis(2-hydroxyethyl)imino]tris(hydroxymethyl)methane (bis-tris) buffers are employed with ph 6.8 and ...200111262168
antimicrobial activity of honokiol and magnolol isolated from magnolia officinalis.the antimicrobial activity of honokiol and magnolol, the main constituents of magnolia officinalis was investigated. the antimicrobial activity was assayed by the agar dilution method using brain heart infusion medium and the minimum inhibitory concentration (mic) were determined for each compound using a twofold serial dilution assay. the results showed that honokiol and magnolol have a marked antimicrobial effect (mic = 25 microg/ml) against actinobacillus actinomycetemcomitans, porphyromonas ...200111268114
synthesis of mannich bases of some 2,5-disubstituted 4-thiazolidinones and evaluation of their antimicrobial activities.5-phenyl/methyl-5-morpholinomethyl/pyrrolidinomethyl-2-(5- aryl-1,3,4-oxadiazol-2-yl)imino]-4-thiazolidinones (5a-m) were synthesized by the reaction of 5-phenyl/methyl-2-[(5-aryl-1,3,4-oxadiazol -2-yl)imino]-4-thiazolidinones (4a-j) with formaldehyde and morpholine or pyrrolidine. the structures of the compounds were determined by analytical and spectral (ir, 1h-nmr, eims) methods. the antibacterial activities of the novel compounds against staphylococcus aureus atcc 6538, staphylococcus epider ...200111268772
the structure of the carbohydrate backbone of the core-lipid a region of the lipopolysaccharide from proteus penneri strain 40: new proteus strains containing open-chain acetal-linked n-acetylgalactosamine in the core part of the lps.analysis of the core part of the lps from several strains of proteus revealed that p. penneri strains 2, 11, 19, 107, and p. vulgaris serotypes 04 and 08 have the same structure with a new type of linkage between monosaccharidesan open-chain acetal--that was previously determined for p. vulgaris ox2 and p. penneri 17. the lps from p. penneri strain 40 contains the same structure substituted with one additional monosaccharide: [molecular structure: see text] where (1s)-galanac1 is a residue of n- ...200111269407
biodiversity and horizontal gene transfer in culturable bacteria isolated from activated sludge enriched in nonylphenol ethoxylates.one hundred and twenty bacterial isolates, from activated sludge of a treatment plant collecting wastes enriched in ethoxylated nonylphenols, were studied. sixty isolates were selected on rich medium and 60 on mineral medium containing two nonylphenol ethoxylates as the sole carbon source. analysis of biodiversity at the species level was performed by comparing the alui restriction patterns of the 16s ribosomal dna amplified by pcr from 120 isolates. the rdna restriction analysis enabled us to c ...200111281319
biochemical identification and characterization of dna groups within the proteus vulgaris complex.we placed 43 isolates belonging to the proteus vulgaris complex into proposed dna groups (genomovars) using five previously recommended tests (tests for esculin hydrolysis, production of acid from salicin, l-rhamnose fermentation, and elaboration of dnase and lipase). on the basis of the results of these five tests, 49% of the isolates fell into dna groups 5 and 6, 37% fell into dna group 2, and the remaining 14% fell into dna groups 3 and 4. sequencing of the 16s rrna genes of 11 members of dna ...200111283033
xanthogranulomatous pyelonephritis in childhood.demographic data, clinical presentation, associated abnormalities, and radiologic findings were evaluated to outline diagnostic criteria that may lead to the diagnosis of xanthogranulomatous pyelonephritis in children.200111283885
intradermal skin test with diabetes specific antigens in patients with type 1 diabetes.cell mediated immune response in vitro to a number of antigens has been reported in patients with type 1 diabetes. the aim of the present study was to develop an in vivo intradermal (delayed type hypersensitivity) skin test using antigens known to be recognized by lymphocytes of patients with type 1 diabetes and to compare, where possible, the in vivo response to the in vitro t cell proliferation to the same antigens. the skin test was performed in the following group of patients: 55 with recent ...200111298123
pemphigus vulgaris and disseminated nocardiosis.infectious diseases, in particular septicaemia from staphylococcus aureus, proteus vulgaris and pseudomonas aeruginosa, are the most severe and frequent complications for the immunosuppressive therapy of pemphigus. infection by nocardia asteroides in subjects with pemphigus vulgaris is rare. we report the sixth case found of such an association; the subject died of disseminated nocardiosis while receiving steroids and immunosuppressive drugs, 4 years after being diagnosed with chronic pemphigus ...200011305389
analytic model for ring pattern formation by bacterial swarmers.we analyze a model proposed by medvedev, kaper, and kopell (the mkk model) for ring formation in two-dimensional bacterial colonies of proteus mirabilis. we correct the model to formally include a feature crucial of the ring generation mechanism: a bacterial density threshold to the nonlinear diffusivity of the mkk model. we numerically integrate the model equations, and observe the logarithmic profiles of the bacterial densities near the front. these lead us to define a consolidation front dist ...200111308679
theory of periodic swarming of bacteria: application to proteus mirabilis.the periodic swarming of bacteria is one of the simplest examples for pattern formation produced by the self-organized collective behavior of a large number of organisms. in the spectacular colonies of proteus mirabilis (the most common species exhibiting this type of growth), a series of concentric rings are developed as the bacteria multiply and swarm following a scenario that periodically repeats itself. we have developed a theoretical description for this process in order to obtain a deeper ...200111308686
zentralblatt für bakteriologie--100 years ago: agglutination and filament formation of proteus bacteria and maternal-fetal transfer of agglutinins. 200111310442
[antimicrobial activity of ciprofloxacin and netilmicin compared to various antibiotics in lome, togo].we report the antimicrobial activity of ciprofloxacin and netilmicin on 577 strains such as s. aureus, pseudomonas, e. coli, salmonella, proteus, klebsiella and enterobacter. isolation and identification were performed by standard methods. disk diffusion tests were performed to evalute the susceptibility. the percentage resistance to ciprofloxacin for bacteria was: e. coli = 15%, enterobacter = 13%, proteus = 10%, pseudomonas = 9%, s. aureus and klebsiella = 4%. the percentage resistance to neti ...200111313234
[xanthogranulomatous pyelonephritis. our experience and review of the literature].ten cases of xanthogranulomatous pyelonephritis (xgp) with diagnostic and therapeutic observation are described and other experiences reported in the literature are analyzed.200011315325
proteus syndrome: a variant with eye involvement. 200111318277
[new pyrrolo-pyrimidine derivatives with antifungal or antibacterial properties in vitro]. 200111320339
variations in the prevalence of strains expressing an extended-spectrum beta-lactamase phenotype and characterization of isolates from europe, the americas, and the western pacific region.to evaluate the prevalence of extended-spectrum beta-lactamase (esbl)-producing strains among species of enterobacteriaceae, a microdilution susceptibility test was performed with strains of klebsiella pneumoniae, escherichia coli, proteus mirabilis, and salmonella species that were isolated as part of the sentry project. the highest percentage of esbl phenotype (defined as a minimum inhibitory concentration [mic] > or =2 microg/ml for ceftazidime, ceftriaxone, or aztreonam) was detected among k ...200111320450
structure of the o-specific polysaccharide of proteus vulgaris o4 containing a new component of bacterial polysaccharides, 4,6-dideoxy-4.a high-molecular-mass o-specific polysaccharide was obtained by mild acid degradation of proteus vulgaris o4 lipopolysaccharide followed by gpc. the polysaccharide was studied by chemical methods along with 1h and 13c nmr spectroscopy, including two-dimensional cosy, tocsy, noesy, h-detected 1h,13c hmqc, and 1h,13c hmbc experiments. solvolysis of the polysaccharide with trifluoromethanesulfonic (triflic) acid resulted in a glcpa-(1 --> 3)-glcnac disaccharide and a novel amino sugar derivative, 4 ...200111322733
structure of the o-specific polysaccharide of proteus mirabilis o16 containing ethanolamine phosphate and ribitol phosphate.the o-specific polysaccharide of proteus mirabilis o16 was studied by 1h and 13c nmr spectroscopy, including 2d cosy, tocsy, noesy, h-detected 1h,13c hmqc, hmqc-tocsy, and 1h,31p hmqc experiments, along with chemical methods. the polysaccharide was found to be a ribitol teichoic acid-like polymer having the following structure [structure: see text].200111322736
the multifaceted challenges of proteus syndrome.proteus syndrome is a rare and sporadic disorder that causes postnatal overgrowth of multiple tissues in a mosaic pattern. the overgrowth can involve skin, subcutaneous tissue, connective tissue (including bone), the central nervous system, and viscera. complications of proteus syndrome include, among others, progressive skeletal deformities, invasive lipomas, benign and malignant tumors, and deep venous thrombosis with pulmonary embolism. care of patients with proteus syndrome presents signific ...200111325326
evidence of in vivo transfer of a plasmid encoding the extended-spectrum beta-lactamase tem-24 and other resistance factors among different members of the family enterobacteriaceae.the epidemiological study of several multidrug-resistant enterobacteriaceae isolated from five patients demonstrated in vivo dissemination of a 100-kb plasmid encoding the extended-spectrum beta-lactamase tem-24 from a clonal strain of enterobacter aerogenes to different strains of klebsiella pneumoniae, escherichia coli, proteus vulgaris, proteus mirabilis, and serratia marcescens.200111326030
16s rdna-based identification of bacteria from conjunctival swabs by pcr and dgge fingerprinting.establishment of a new molecular biology technique for the identification of multiple bacteria from the ocular environment, which can be applied supplementarily to cultivation in cases of severe bacterial infections.200111328723
transferable antibiotic resistance in multiresistant nosocomial acinetobacter baumannii strains from seven clinics in the slovak and czech republics.sixty-seven multiresistant nosocomial acinetobacter baumannii isolates from patients hospitalized mostly in intensive care units of seven clinics in slovak and czech republic were tested to determine their ability to transfer antibiotic resistance. all isolates were resistant to kanamycin, ticarcillin, cephalothin, cefotaxime, ceftazidime, aztreonam and susceptible to carbapenems, sulbactam and ampicillin/sulbactam. sixty-five out of 67 strains transferred resistance determinants to escherichia ...200111330360
antibiotic selective pressure and development of bacterial resistance.this study evaluates the development of resistance in gram-negative rods to cefotaxime and ceftazidime, ofloxacin and ciprofloxacin, gentamicin and amikacin, meropenem and ampicillin/sulbactam over a five year period of use (1994-1998) at the university hospital in olomouc, czech republic. the development of bacterial resistance was linked with antibiotic use and hence selective pressure which was specific for the type of antibiotic and the bacterial species. statistically significant correlatio ...200111337221
[comparative study of the effects of different sour milk products on the intestinal microflora of healthy infants].our study was aimed to investigate in comparable conditions the composition of intestinal microflora of healthy infants who were given 9 types of fermented milk products. there were observed 103 practically healthy infants aged from 4 month to 1,5 years old. the obtained data have shown that studied products improve intestinal microflora in infants. significant increase of bifidobacteria in infants intestines was promoted by fermented milk products containing alive bifidobacteria with rather hig ...200111338340
causes of premature death in proteus syndrome. 200111343327
leukocyte antimicrobial function in patients with leprosy.patients with lepromatous leprosy are unresponsive to lepromin skin-test material and possess defective lymphocyte function in vitro, including impaired mitogenesis in response to antigens of mycobacterium leprae. it has been claimed that their macrophages cannot digest m. leprae in vitro; such a defect could explain both lepromin nonreactivity and impaired lymphocyte function on the basis of failure of the afferent limb of the immune response (i.e., defective macrophage "processing" of m. lepra ...197411344550
proteus mirabilis septic monoarthritis in a patient with cpdd. 200111344817
structure and serological characterization of an nepsilon-[(r)-1-carboxyethyl]-l-lysine-containing o-chain of the lipopolysaccharide of proteus mirabilis o13.in this paper we present the structure and describe serological properties of the o-specific polysaccharide of proteus mirabilis o13 lipopolysaccharide, which contains a unique component: an amide of d-galacturonic acid (d-gala) with an unusual amino acid, nepsilon-[(r)-1-carboxyethyl]-l-lysine (alaninolysine, alalys). selective chemical degradations of either gala or alalys resulted in the loss of the serological reactivity of the polysaccharide with anti-o serum against p. mirabilis o13. neith ...200111348021
fluorogenic assay for penicillin g acylase activity.a simple, highly sensitive, and rapid assay for high-throughput screening of penicillin g acylase-producing bacteria is presented. the method is based on the specific release of fluorescent 7-amino-4-methyl-coumarin through cleavage of phenylacetyl-4-methyl-coumaryl-7-amide by penicillin g acylase. the present method is suitable for screening pure enzymes as well as various penicillin g acylases like those from escherichia coli, proteus rettgeri, and kluyvera citrophila in cell extracts. in addi ...200111355855
[phosphatase from proteus mirabilis].cell-free preparations of proteus mirabilis contained a phosphatase (ec 3.1.3.1), whose activity surpassed that of alkaline phosphatase from escherichia coli. phosphatase was also found in the culture liquid of p. mirabilis. the composition of proteins displaying enzyme activity was assayed by polyacrylamide gel electrophoresis. enzyme synthesis was studied at various stages of bacterial growth. biosynthesis of phosphatase in p. mirabilis (similarly to that in other bacteria) was shown to be ind ...200111357420
the effect of resveratrol on the platelet secretory process induced by endotoxin and thrombin.the effect of resveratrol (trans-3,4',5-trihydroxystilbene) on the release of adenine nucleotides and proteins from blood platelets activated by lipopolysaccharide (lps), from proteus mirabilis and by thrombin, were studied. thrombin stimulated the release of adenine nucleotides from dense granules and proteins from alpha-granules. the lps (0.3 microg/10(8) platelets, 5 min, 37 degrees c), like thrombin (2.5 u/10(8) platelets, 5 min, 37 degrees c) was found to cause a release of adenine nucleoti ...200111368092
microbiology of chronic suppurative otitis media with cholesteatoma.to report the result of causative organisms in chronic suppurative otitis media with cholesteatoma.200011369953
the influence of plasma on the disinfecting activity of the new antimicrobial agent n-chlorotaurine-sodium in comparison with chloramine t.the phenomenon of increasing bactericidal activity of n-chlorotaurine in the presence of chlorine-consuming material has been investigated both on a chemical-analytical and microbiological basis using plasma as substrate and chloramine t for comparison. chlorine consumption assessed by iodometric titration showed a biphasic time-course with a very fast loss of oxidation capacity within one minute (n-chlorotaurine: -9.3%, chloramine t: -16.8%) followed by a slow loss which could still be detected ...200111370708
genetic characterization of the klebsiella pneumoniae waa gene cluster, involved in core lipopolysaccharide biosynthesis.a recombinant cosmid containing genes involved in klebsiella pneumoniae c3 core lipopolysaccharide biosynthesis was identified by its ability to confer bacteriocin 28b resistance to escherichia coli k-12. the recombinant cosmid contains 12 genes, the whole waa gene cluster, flanked by kbl and coad genes, as was found in e. coli k-12. pcr amplification analysis showed that this cluster is conserved in representative k. pneumoniae strains. partial nucleotide sequence determination showed that the ...200111371519
[pattern of antimicrobial susceptibility in uropathogen isolates from children].the bacteriological profile in children with culture-positive bacteriuria was analyzed during a 5-year period. escherichia coli was the most common cause of urinary tract infections (57%), followed by streptococcus faecalis (11%), pseudomonas aeruginosa (8%), and proteus mirabilis (6%). results of antimicrobial susceptibility testing indicated that cephalosporins (first, second and third generation) and nitrofurantoin are the best empirical oral treatment for urinary infections in children. fosf ...200111376352
phenotypic variability of beta-glucoside utilization and its correlation to pathogenesis process in a few enteric bacteria.utilization of beta-glucosides is markedly variable in the members of the family enterobacteriaceae. the results presented here provide molecular clues for evolutionary events that resulted in the phenotypic variability seen amongst the members of these species. the genomic hybridization of selected enterobacteriaceae members with the escherichia coli bgl and cel genes resulted in detection of a complete homolog of the bgl and cel operons in shigella sonnei, a member that is evolutionarily close ...200111377874
[isolation of glutamylendopeptidase precursor from b. licheniformis and its processing in vitro].a secretory system based on l-form cells of proteus mirabilis was developed for production of native bacillus licheniformis glutamylendopeptidase precursor never formerly available. the produced precursor was stable per se under physiological conditions and in presence of trypsin and glutamylendopeptidase from b. intermedius. complete conversion of the precursor to the mature glutamylendopeptidase was performed by bacillar metalloproteases and subtilisin. the artificially processed glutamylendop ...200111385993
antibiotic susceptibility of bacterial strains isolated from urinary tract infections in poland.worldwide data show that there is increasing resistance among urinary tract pathogens to conventional drugs. the aim of this study was to obtain data on susceptibility patterns of pathogens responsible for urinary tract infections (utis) in poland to currently used antimicrobial agents. a multicentre study of 141 pathogens from hospital-acquired infections and 460 pathogens from community-acquired infections was carried out between july 1998 and may 1999. the most prevalent aetiological agent wa ...200111389109
prior trimethoprim use and trimethoprim-resistant urinary tract infection: a nested case-control study with multivariate analysis for other risk factors.trimethoprim resistance is increasingly prevalent in community-acquired urinary infections. the objective of this study was to evaluate the association between exposure to community-prescribed trimethoprim and other risk factors in subjects and subsequent trimethoprim-resistant urinary tract infection. the design was a nested case-control study using a record-linkage database. study subjects submitted a urine sample to the ninewells hospital laboratory between july 1993 and december 1995. antibi ...200111389110
integrin expression, enterocyte maturation, and bacterial internalization.little is known about the molecular mechanisms involved in the translocation of enteric bacteria. adhesion molecules mediate interactions between some enteric pathogens and mammalian cells, but no such interactions have been identified for enterocytes and normal enteric bacteria. using enteric pathogens, adhesion molecule expression has been linked to bacterial internalization and to enterocyte differentiation. therefore, experiments were designed to study enterocyte integrin expression and diff ...200111397127
surface sensing, swarmer cell differentiation, and biofilm development. 200111398406
the improbable "elephant man". 200111399837
novel type of fimbriae encoded by the large plasmid of sorbitol-fermenting enterohemorrhagic escherichia coli o157:h(-).sorbitol-fermenting (sf) enterohemorrhagic escherichia coli (ehec) o157:h(-) have emerged as important causes of diarrheal diseases and the hemolytic-uremic syndrome in germany. in this study, we characterized a 32-kb fragment of the plasmid of sf ehec o157:h(-), psfo157, which differs markedly from plasmid po157 of classical non-sorbitol-fermenting ehec o157:h7. we found a cluster of six genes, termed sfpa, sfph, sfpc, sfpd, sfpj, and sfpg, which mediate mannose-resistant hemagglutination and t ...200111401985
presence of escherichia coli o157:h7 in ground beef and ground baby beef meat.a total of 114 beef and baby beef samples were examined. the samples included ground baby beef, mixed ground baby beef and pork, and chopped and shaped meat. the samples were analyzed from 30 different grocery stores in zagreb, croatia. the object of this study was to evaluate the prevalence of escherichia coli o157:h7 in the samples that can enhance the potential risk of outbreaks of hemorrhagic colitis and hemolytic uremic syndrome. the results in all tested samples of e. coli o157:h7 were neg ...200111403139
[effect of bioceramics on biological activity of microorganisms].biomaterials that come into contact with microorganisms in the middle ear or paranasal sinuses should be tested before clinical application. thus, it is necessary to test the influence exerted on bacterial growth by biomaterials used as bone substitutes in head and neck surgery before implantation.200111405144
postantibiotic suppression effect of macrolides on the expression of flagellin in pseudomonas aeruginosa and proteus mirabilis.the phenomenon of postantibiotic effect (pae) encompasses not only the effects of bacterial growth inhibition but also the suppression of virulence factors. we tentatively designated the latter effect the postantibiotic suppression effect (pase). the flagella of gram-negative bacteria are involved in the development of biofilms. we measured the pase of erythromycin (ery) and azithromycin (azm) on the expression of flagellin in pseudomonas aeruginosa and proteus mirabilis. flagellin preparations ...200111406758
identification of proteus mirabilis mutants with increased sensitivity to antimicrobial peptides.antimicrobial peptides (aps) are important components of the innate defenses of animals, plants, and microorganisms. however, some bacterial pathogens are resistant to the action of aps. for example, proteus mirabilis is highly resistant to the action of aps, such as polymyxin b (pm), protegrin, and the synthetic protegrin analog ib-367. to better understand this resistance, a transposon mutagenesis approach was used to generate p. mirabilis mutants sensitive to aps. four unique pm-sensitive mut ...200111408219
tissue antioxidant capacity and bacterial translocation under total parenteral nutrition.alterations in the antioxidative system have been observed during total parenteral nutrition (tpn). light exposure or changes in the composition of tpn formulas may affect this system. bacterial translocation (bt) is frequent under tpn and may be related to oxidative status. the aim of this study was to determine the adverse effects of standard and glutamine-enriched tpn, with or without light exposure, on oxidative status (liver and kidney-reduced glutathione, gsh) and its relationship to bt. t ...200111409162
biotransformation of crotonobetaine to l(-)-carnitine in proteus sp.two proteins, component i (ci) and component ii (cii), catalyze the biotransformation of crotonobetaine to l(-)-carnitine in proteus sp. ci was purified to electrophoretic homogeneity from cell-free extracts of proteus sp. the n-terminal amino acid sequence of ci showed high similarity (80%) to the caib gene product from escherichia coli o44k74, which encodes the l(-)-carnitine dehydratase. ci alone was unable to convert crotonobetaine into l(-)-carnitine even in the presence of the cosubstrates ...200111409545
a putative insect intracellular endosymbiont stem clade, within the enterobacteriaceae, infered from phylogenetic analysis based on a heterogeneous model of dna evolution.insect intracellular symbiotic bacteria (intracellular endosymbionts, or endocytobionts) were positioned within the gamma 3-proteobacteria using a non-homogeneous model of dna evolution, allowing for rate variability among sites, for gc content heterogeneity among sequences, and applied to a maximum likelihood framework. most of them were found to be closely related within the enterobacteriaceae family, located between proteus and yersinia. these results suggest that such a bacterial group might ...200111411291
bacterial flora of the conjunctiva and nasal cavity in normal and diseased captive bustards.a survey was carried out to describe the normal aerobic bacterial flora of the conjunctiva and nasal cavity of captive houbara bustards (chlamydotis undulata), kori bustards (ardeotis kori), and white-bellied bustards (eupodotis senegalensis) maintained at the national avian research center, abu dhabi, united arab emirates. a total of 58 samples were examined from the nasal cavity and 55 samples from the conjunctiva of healthy bustards. there was no bacterial growth in 45% of conjunctival sample ...200111417826
activity of levofloxacin and ciprofloxacin against urinary pathogens.this study compares the antibacterial activities of levofloxacin and ciprofloxacin against recently isolated urinary tract pathogens, by evaluating their mics and mbcs in accordance with nccls susceptibility tests, time-kill curves and interference with bacterial adhesion to uroepithelial cells. a total of 200 clinical isolates was tested, including the species escherichia coli, klebsiella pneumoniae, proteus mirabilis, proteus vulgaris, providencia rettgeri, pseudomonas aeruginosa, enterococcus ...200111418511
proteus syndrome. 200111418739
the development of the bsac standardized method of disc diffusion testing.the bsac working party on susceptibility testing has developed a standardized method of disc susceptibility testing that has been 'field tested' in 19 diagnostic laboratories in the uk and ireland. the method employs semi-defined media, a semi-confluent inoculum and relates zones of inhibition with bsac-specified mic breakpoints to interpret susceptibility. the bacteria selected for the trial included clinical isolates and control strains from the atcc and nctc national collections. organisms we ...200111420335
[increased antibacterial activity of antibiotics with etonium in vitro].the activity of compositions of antibiotics cefasolin, benzylpenicillin and gentamycin with etonium in respect to museum strains staphylococcus aureus 209, escherichia coli k-12, proteus vulgaris 410, p. mirabilis, pseudomonas aeruginosa 19, klebsiella pneumoniae 5054 and polyresistance strains s. aureus, e. coli, p. mirabilis in vitro was researched. the increase of the compositions activity as a result of synergy in the action of their component 4 up to 4496 times has been established.200011421003
antimicrobial agent resistance in bacterial isolates from patients with diarrhea and urinary tract infection in the sudan.antimicrobial sensitivity tests were performed on four-hundred and ninety-seven bacterial isolates from sudanese patients with diarrhea or urinary tract infections. shigella dysenteriae type i and enteropathogenic escherichia coli showed high resistance rates (percentage of isolates showing antibiotic resistance) against the commonly-used antimicrobial agents: ampicillin, amoxicillin, chloramphenicol, tetracycline, cotrimoxazole, nalidixic acid, sulfonamide, and neomycin, and were completely sen ...200011421374
urinary tract infections in general practice in a rural community in south trinidad.a prospective study was carried out at a general practice in south trinidad, to assess the sensitivity profiles of urinary isolates in this remote rural community.200111426248
longitudinal melanonychia.ungual melanoma is the most serious disease affecting the nail. the majority start with a longitudinal brown streak in the nail.200111442597
urinary tract infections in women.urinary tract infections (utis) are the most common infections seen in the hospital setting, and the second most common infections seen in the general population. due to women's anatomy, utis are especially problematic for them, and up to one-third of all women will experience a uti at some point during their lifetimes. appropriate treatment of a uti requires accurate classification that includes infection site, complexity of the infection, and the likelihood of recurrence. the predominant patho ...200111442991
identification of the domains of urer, an arac-like transcriptional regulator of the urease gene cluster in proteus mirabilis.proteus mirabilis urease catalyzes the hydrolysis of urea to co(2) and nh(3), resulting in urinary stone formation in individuals with complicated urinary tract infections. urer, a member of the arac family, activates transcription of the genes encoding urease enzyme subunits and accessory proteins, uredabcefg, as well as its own transcription in the presence of urea. based on sequence homology with arac, we hypothesized that urer contains both a dimerization domain and a dna-binding domain. a t ...200111443087
robotic enzyme amplification: a comparison of some kinetic properties of bovine liver, candida utilis and proteus sp. glutamic dehydrogenases.nadp(h)-specific bakers yeast glucose 6-phosphate dehydrogenase (byg6pdh) was paired, in turn, with each of three different source glutamate dehydrogenases (gdhs): nad(p)-specific bovine liver (blgdh), nadp-specific candida utilis (cugdh) and nadp-specific proteus sp. (psgdh) to constitute three enzyme cycling systems; (i) byg6pdh/blgdh; (ii) byg6pdh/cugdh; and (iii) byg6pdh/psgdh. when incorporated into an enzymatic cycling/amplification system for nad kinase and run on a centrifugal fast analy ...200111445951
[escherichia coli eradication from the blader urine by amoxicillin-sulbactam. intrinsic activity of the inhibitor].at urine concentrations obtained after the oral administration of amoxicillin-sulbactam (500/500 mg) this combination inhibits roughly 90% of escherichia coli and proteus mirabilis strains.200111446908
comparative in vitro activity of gemifloxacin, ciprofloxacin, levofloxacin and ofloxacin in a north american surveillance study.the in vitro activity of gemifloxacin, a new fluoroquinolone, was compared to three marketed fluoroquinolones; ciprofloxacin, levofloxacin and ofloxacin against over 4,000 recent clinical isolates covering 29 species isolated in the united states and canada between 1997-1999. based on mic(90)s, gemifloxacin was the most potent fluoroquinolone tested against a majority of gram-positive isolates: streptococcus pneumoniae, penicillin resistant s. pneumoniae, macrolide resistant s. pneumoniae, cipro ...200111448564
structure of the acidic o-specific polysaccharide from proteus vulgaris o39 containing 5,7-diacetamido-3,5,7,9-tetradeoxy-l-glycero-l-manno-non-2-ulosonic acid.the o-specific polysaccharide of proteus vulgaris o39 was found to contain a new acidic component of proteus lipopolysaccharides, 5,7-diacetamido-3,5,7,9-tetradeoxy-l-glycero-l-manno-non-2-ulosonic acid (di-n-acetylpseudaminic acid, pse5ac7ac). the following structure of the polysaccharide was determined by nmr spectroscopy, including 2d 1h,(1)h cosy, tocsy, roesy, and 1h,(13)c hmqc experiments, along with selective cleavage of the polysaccharide by solvolysis with anhydrous trifluoromethanesulf ...200111448686
antibacterial and molluscicidal activities of the essential oil of chrysanthemum viscidehirtum.the volatile fraction of chrysanthemum viscidehirtum aerial parts, consisting mainly of limonene, beta-farnesene and many oxygenated sesquiterpenes, was screened for activity against 21 microbial strains. this essential oil exhibited activity against all germs tested, in particular salmonella typhi and proteus mirabilis. it also showed molluscicidal activity against bulinus truncatus.200011449503
novel class a beta-lactamase sed-1 from citrobacter sedlakii: genetic diversity of beta-lactamases within the citrobacter genus.citrobacter sedlakii 2596, a clinical strain resistant to aminopenicillins, carboxypenicillins, and early cephalosporins such as cephalothin, but remaining susceptible to acylureidopenicillins, carbapenems, and later cephalosporins such as cefotaxime, was isolated from the bile of a patient treated with beta-lactam and quinolone antibiotics. the isolate produced an inducible class a beta-lactamase of pi 8.6, named sed-1, which was purified. characterized by a molecular mass of 30 kda, sed-1 pref ...200111451687
symptomatic diffuse colonic lipomatosis in proteus syndrome. 200111454051
the generation of superoxide anion in blood platelets in response to different forms of proteus mirabilis lipopolysaccharide: effects of staurosporin, wortmannin, and indomethacin.lipopolysaccharide (lps), the major component of the outer membrane of gram-negative bacteria may activate blood platelets. the aim of our study was to evaluate the effects of different forms of proteus mirabilis lps and isolated lipid a and polysaccharide part on the production of superoxide radicals in blood platelets and to estimate the role staurosporin, wortmannin and indomethacin on this process. we compared the generation of superoxide radicals in platelets treated with lps after preincub ...200111457473
new antioxidant and antimicrobial ellagic acid derivatives from pteleopsis hylodendron.bioassay-guided isolation of two new compounds, 3,4-methylenedioxy-3'-o-methyl-4'-o-glucoside ellagic acid (1) and the pteleoellagic acid derivative (2), from the stem bark of pteleopsis hylodendron is reported along with 3,4-methylenedioxy-3'-o-methyl ellagic acid (3), 3,3'-di-o-methyl ellagic acid (4) and 3,3',4'-tri-o-methyl ellagic acid (5), which were obtained for the first time from this plant. the structures of these compounds were elucidated with the help of spectroscopic studies. compou ...200111458450
the effects of pentoxifylline treatment on bacterial translocation after hemorrhagic shock in rats.bacterial translocation is thought to be responsible for infectious complications after hemorrhagic shock. the aim of this study was to investigate the effects of pentoxifylline treatment on bacterial translocation in animals subjected to hemorrhagic shock. thirty-one wistar albino rats (280-360 g) were divided into three groups: sham (n=10), shock (n=11), and shock-pentoxifylline (n=10). blood was not withdrawn from sham rats. shock rats were subjected to 30 min of shock followed by reinfusion ...200111467403
interrelations of organism prevalence, specimen collection method, and host age, sex, and breed among 8,354 canine urinary tract infections (1969-1995).selected information was compiled from canine urinalyses and urine cultures conducted between january 1969 and december 1995. eight thousand three hundred fifty-four microbial isolates (bacteria and fungi) included 4,873 isolates from females and 3,481 from males. ten bacterial genera accounted for 96.3% of the urinary isolates, including escherichia coli (44.1%), staphylococcus spp. (11.6%), proteus spp. (9.3%), klebsiella spp. (9.1%), enterococcus spp. (8.0%), and streptococcus spp. (5.4%) as ...200111467591
the effect of ship scrapping industry and its associated wastes on the biomass production and biodiversity of biota in in situ condition at alang.the main pollutants for the ship scrapping industry and its associated wastes at alang are heavy metals, petroleum hydrocarbon and bacterial contaminations. the concentration of iron, manganese, cobalt, copper, zinc, lead, cadmium, nickel and mercury were 25 to 15,500% more at nearshore station of alang as compared to control site at piram. the concentration of heavy metals in the nearshore station of alang was always higher than its concentration at 10 km away. the concentration of petroleum hy ...200111468924
asymmetry: molecular, biologic, embryopathic, and clinical perspectives.this overview of asymmetry addresses the following topics: chiral molecules; asymmetric signaling molecules, including n-cadherin, shh, fgf8, lefty1, lefty2, nodal, pitx2, activin betab, activin receptor iia, and csnr; situs abnormalities; asymmetric cell division; laterality in humans and animals; behavioral asymmetry in humans and animals; asymmetric embryopathies, including tessier-type "clefts"; hemiasymmetries such as hemihyperplasia, hemihypoplasia, and hemiatrophy; asymmetric vascular syn ...200111471152
the formulation of an effective topical antibacterial product containing ocimum gratissimum leaf essential oil.the antibacterial potential of ocimum gratissimum essential oil was explored. liquid and semisolid formulations of the oil were designed in a variety of bases for topical antiseptic medication. the products were evaluated by agar diffusion assay against type strains and clinical isolates from boil, wound and pimples. remarkable antibacterial effects, higher than those of commercial antiseptic products, were demonstrated at 2% ocimum oil concentration in some bases. the properties of base into wh ...200111472827
advances in disinfection testing and modelling.to develop a set of kinetic equations which more ably describe the disinfection process.200111473601
[black nails caused by proteus mirabilis].black nails caused by proteus mirabilis were seen in a motor mechanic and in a petrol pump attendant. proteus mirabilis is a gram-negative bacillus that generates hydrogen sulfide. this compound reacts with traces of metals in the nail plate such as zinc, nickel, cobalt, iron, manganese, tin, copper and lead. metal sulfides blacken the nail plate. the protracted course of the discoloration over months corresponds to the slow reactions of metals with hydrogen sulfide. the disappearance of the bla ...200111475651
blood stream infections in a medical intensive care unit: spectrum and antibiotic susceptibility pattern.to determine the type and sensitivity pattern of causative organisms of septicaemia in intensive care unit, to prepare a guideline for empirical antibiotic therapy.200111475775
pten mutations are uncommon in proteus syndrome. 200111476065
association of germline mutation in the pten tumour suppressor gene and proteus and proteus-like syndromes.the molecular aetiology of proteus syndrome (ps) remains elusive. germline mutations in pten cause cowden syndrome and bannayan-riley-ruvalcaba syndrome, which are hereditary hamartoma syndromes. some features-eg, macrocephaly, lipomatosis, and vascular malformations-can be seen in all three syndromes. we examined pten in patients with ps and undefined proteus-like syndromes (ps-like) and identified de-novo germline mutations in two of nine patients with ps and three of five patients with ps-lik ...200111476841
etiologies of the urinary tract infections in a yemeni city.to determine the causative agents of urinary tract infection in yemeni patients in sana'a city, and to determine the antibiotic susceptibilities of these agents in vitro.200111479641
coronal leakage following three obturation techniques.to compare coronal bacterial and india ink leakage in three different obturation techniques with the smear layer having been removed.200111482141
antimicrobial activity of alstonia macrophylla: a folklore of bay islands.the methanolic crude and methanol-aqueous extract of alstonia macrophylla leaves and n-butanol part of the crude extract showed antimicrobial activity against various strains of staphylococcus aureus, staphylococcus saprophyticus, streptococcus faecalis, escherichia coli, proteus mirabilis, trichophyton rubrum, trichophyton mentagrophytes var. mentagrophytes and microsporum gypseum. the minimum inhibitory concentration (mic) values ranges from 64 to 1000 microg/ml for bacteria and 32-128 mg/ml f ...200111483378
dental unit waterlines: review and product evaluation.dental practitioners must be knowledgeable regarding microbial contamination and biofilm formation in dental unit waterlines. education should stress the need for improvement in the quality of water delivered to patients during treatment. manufacturers must also play an important role by providing training and education regarding the proper use and maintenance of their systems. dental facilities, both public and private, need reliable methods to prevent the development of biofilms within duws. t ...200111484611
[clinico-laboratory parallels during celiac disease in children].forty-five patients with celiac disease and 73 with the celiakia syndrome were observed. the clinical picture was identical and was characterized by disordered intestinal absorption. depression of t and b lymphocytes was typical of both forms of the disease. high values of antigliadin iga and igg correlated with the severity of atrophic processes in the small intestinal mucosa. intestinal microflora was characterized by a decrease in the main defense flora and growth of hemolytical and lactonega ...200011486746
does drinking cranberry juice produce urine inhibitory to the development of crystalline, catheter-blocking proteus mirabilis biofilms?to test the recommendation that to avoid the complications of long-term indwelling bladder catheterization (e.g. encrustation and blockage by crystalline proteus mirabilis biofilms) patients should drink cranberry juice.200111488728
structure of the o-specific polysaccharide of proteus mirabilis d52 and typing of this strain to proteus serogroup o33.the acidic o-specific polysaccharide chain (o-antigen) of the lipopolysaccharide (lps) of proteus mirabilis strain d52 was studied using chemical analyses along with 1h-nmr and 13c-nmr spectroscopy, including 2d cosy, tocsy, roesy, h-detected 1h,13c and 1h,31p hmqc experiments. the polysaccharide was found to contain d-ribitol 5-phosphate (d-rib-ol-5-p) and ethanolamine phosphate (etn-p) and has the following structure: d-rib-ol-5-p (3) approximately 75% etnp(6)-->2)-beta-d-galp-(1-->3)-alpha-d- ...200111488930
further studies on rpos in enterobacteria: identification of rpos in enterobacter cloacae and kluyvera cryocrescens.rpos, the alternative sigma factor sigma(s), is important for bacterial survival under extreme conditions. many enterobacteria are opportunistic human pathogens and their ability to survive in a changing environment could be an essential step for their virulence. to determine the presence of this gene in enteric bacteria, an escherichia coli rpos probe was constructed and used to detect the presence of this gene in different species. a gene homologous to rpos was found in citrobacter amalonaticu ...200111491080
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