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Phylogenomics of Reichenowia parasitica, an Alphaproteobacterial Endosymbiont of the Freshwater Leech Placobdella parasitica.Although several commensal alphaproteobacteria form close relationships with plant hosts where they aid in (e.g.,) nitrogen fixation and nodulation, only a few inhabit animal hosts. Among these, Reichenowia picta, R. ornata and R. parasitica, are currently the only known mutualistic, alphaproteobacterial endosymbionts to inhabit leeches. These bacteria are harbored in the epithelial cells of the mycetomal structures of their freshwater leech hosts, Placobdella spp., and these structures have no ...201122132238
microbial sucrose isomerases: producing organisms, genes and enzymes.sucrose isomerase (si) activity is used industrially for the conversion of sucrose into isomers, particularly isomaltulose or trehalulose, which have properties advantageous over sucrose for some food uses. all of the known microbial sis are tim barrel proteins that convert sucrose without need for any cofactors, with varying kinetics and product specificities. the current analysis was undertaken to bridge key gaps between the information in patents and scientific publications about the microbes ...201222133441
effect of fungicides on plant growth promoting activities of phosphate solubilizing pseudomonasputida isolated from mustard (brassica compestris) rhizosphere.this study was navigated to examine the effects of fungicide-stress on the activities of plant-growth-promoting rhizobacterium pseudomonasputida with inherent phosphate solubilizing activity. the fungicide-tolerant and phosphate solubilizing p.putida strain ps9 was isolated from the mustard rhizosphere and tentatively identified following standard morphological, physiological and biochemical tests. to further consolidate the identity of the strain ps9, the 16s rdna sequence analysis was performe ...201122133911
isolation and functional gene analyses of aromatic-hydrocarbon-degrading bacteria from a polychlorinated-dioxin-dechlorinating process.aerobic aromatic-hydrocarbon-degrading bacteria from a semi-anaerobic microbial microcosm that exhibited apparent complete dechlorination of polychlorinated dibenzo-p-dioxins/dibenzofurans (pcdd/fs) were isolated through enrichment and plating culture procedures with dibenzofuran as the model substrate. by 16s rrna gene sequence comparisons, these dibenzofuran-degrading isolates were identified as being members of the phyla actinobacteria, firmicutes, and proteobacteria, among which those of the ...201122146307
Mutation of the sensor kinase chvG in Rhizobium leguminosarum negatively impacts cellular metabolism, outer membrane stability, and symbiosis.Two component signal transduction systems (TCS) are a main strategy used by bacteria to sense and adapt to changes in their environment. In the legume symbiont Rhizobium leguminosarum biovar viciae VF39, mutation of the histidine kinase, chvG, caused a number of pleiotropic phenotypes. ChvG mutants are unable to grow on proline, glutamate, histidine, or arginine as the sole carbon source. The chvG mutant secreted lower amounts of acidic and neutral surface polysaccharides, and accumulated abnorm ...201122155778
international committee on systematics of prokaryotes subcommittee on the taxonomy of agrobacterium and rhizobium: minutes of the meeting, 7 september 2010, geneva, switzerland. 201122156799
polyphasic approach for the characterization of rhizobial symbionts effective in fixing n(2) with common bean (phaseolus vulgaris l.).common bean (phaseolus vulgaris l.) is a legume that has been reported as highly promiscuous in nodulating with a variety of rhizobial strains, often with low effectiveness in fixing nitrogen. the aim of this work was to assess the symbiotic efficiency of rhizobial strains isolated from common bean seeds, nodules of arachis hypogaea, mucuna pruriens, and soils from various brazilian agroecosystems, followed by the characterization of elite strains identified in the first screening. forty-five el ...201122159885
Environmental signals and regulatory pathways that influence exopolysaccharide production in rhizobia.Rhizobia are Gram-negative bacteria that can exist either as free-living bacteria or as nitrogen-fixing symbionts inside root nodules of leguminous plants. The composition of the rhizobial outer surface, containing a variety of polysaccharides, plays a significant role in the adaptation of these bacteria in both habitats. Among rhizobial polymers, exopolysaccharide (EPS) is indispensable for the invasion of a great majority of host plants which form indeterminate-type nodules. Various functions ...201122174640
mutualism and adaptive divergence: co-invasion of a heterogeneous grassland by an exotic legume-rhizobium symbiosis.species interactions play a critical role in biological invasions. for example, exotic plant and microbe mutualists can facilitate each other's spread as they co-invade novel ranges. environmental context may influence the effect of mutualisms on invasions in heterogeneous environments, however these effects are poorly understood. we examined the mutualism between the legume, medicago polymorpha, and the rhizobium, ensifer medicae, which have both invaded california grasslands. many of these inv ...201122174755
Identification of a novel gene for biosynthesis of a bacteroid-specific electron carrier menaquinone.Ubiquinone (UQ) has been considered as an electron mediator in electron transfer that generates ATP in Rhizobium under both free-living and symbiosis conditions. When mutated, the dmtH gene has a symbiotic phenotype of forming ineffective nodules on Astragalus sinicus. The gene was isolated from a Mesorhizobium huakuii 7653R transposon-inserted mutant library. The DNA sequence and conserved protein domain analyses revealed that dmtH encodes demethylmenaquinone (DMK) methyltransferase, which cata ...201122194970
Roles of secreted peptides in intercellular communication and root development.Intercellular signaling networks control cell identity and activity in all multicellular organisms. Secreted peptides that function as extracellular ligands play essential roles in local communication between adjacent plant cells. The extracellular domain of receptor kinases bind to secreted peptides and initiate downstream cellular responses, resulting in cell proliferation, growth, or differentiation in multicellular organisms. Root growth and development are highly organized processes involvi ...201222195583
Could petroleum biodegradation be a joint achievement of aerobic and anaerobic microrganisms in deep sea reservoirs?ABSTRACT: Several studies suggest that petroleum biodegradation can be achieved by either aerobic or anaerobic microorganisms, depending on oxygen input or other electron acceptors and appropriate nutrients. Evidence from in vitro experiments with samples of petroleum formation water and oils from Pampo Field indicate that petroleum biodegradation is more likely to be a joint achievement of both aerobic and anaerobic bacterial consortia, refining our previous observations of aerobic degradation. ...201122196374
microvirga lupini sp. nov., microvirga lotononidis sp. nov., and microvirga zambiensis sp. nov. are alphaproteobacterial root nodule bacteria that specifically nodulate and fix nitrogen with geographically and taxonomically separate legume hosts.strains of gram-negative, rod-shaped, non-spore-forming bacteria were isolated from nitrogen-fixing nodules of the native legumes listia angolensis (from zambia) and lupinus texensis (from texas, usa). phylogenetic analysis of the 16s rrna gene showed that the novel strains belong to the genus microvirga, with 96.1 % or greater sequence similarity with type strains of this genus. the closest relative of the representative strains lut6t and wsm3557t was m. flocculans tfbt, with 97.6-98.0 % simila ...201122199210
catenulispora graminis sp. nov., rhizobacterium from bamboo (phyllostachys nigro var. henonis) rhizosphere soil.a novel actinobacteria, designated strain br-34(t), was isolated from rhizosphere soil of bamboo (phyllostachys nigro var. henonis) was sampled in damyang, korea. the strain was found to have morphological and chemotaxonomic characteristics typical of the genus catenulispora. the strain contained iso-c(16:0) as major fatty acids and mk-9 (h(4)), mk-9 (h(6)) and mk-9 (h(8)) as the major isoprenoid quinone. phylogenetic analysis of the 16s rrna gene sequence of strain br-34(t) was compared with th ...201122199217
role for rhizobium rhizogenes k84 cell envelope polysaccharides in surface interactions.rhizobium rhizogenes strain k84 is a commercial biocontrol agent used worldwide to control crown gall disease. the organism binds tightly to polypropylene substrate and efficiently colonizes root surfaces as complex, multilayered biofilms. a genetic screen identified two mutants in which these surface interactions were affected. one of these mutants failed to attach and form biofilms on the abiotic surface; although, interestingly, it exhibited normal biofilm formation on the biological root tip ...201122210213
a naturally associated rhizobacterium of arabidopsis thaliana induces a starvation-like transcriptional response while promoting growth.plant growth promotion by rhizobacteria is a known phenomenon but the underlying mechanisms are poorly understood. we searched for plant growth-promoting rhizobacteria that are naturally associated with arabidopsis thaliana to investigate the molecular mechanisms that are involved in plant growth-promotion. we isolated a pseudomonas bacterium (pseudomonas sp. g62) from roots of field-grown arabidopsis plants that has not been described previously and analyzed its effect on plant growth, gene exp ...201122216267
new beta rhizobial strains able to efficiently nodulate parapiptadenia rigida (benth.) brenan.among the leguminous trees natives to uruguay parapiptadenia rigida (angico), a mimosoideae legume, is one of the most promising species for agroforestry. like many other legumes, it is able to establish symbiotic associations with rhizobia and belongs to the group known as "nitrogen fixing trees", which are major components of agroforestry systems. information about rhizobial symbionts for this genus is scarce, and thus the aim of this work was to identify and characterize rhizobia associated w ...201222226956
mercury-resistant rhizobial bacteria isolated from nodules of leguminous plants growing in high hg-contaminated soils.a survey of symbiotic bacteria from legumes grown in high mercury-contaminated soils (almadén, spain) was performed to produce a collection of rhizobia which could be well adapted to the environmental conditions of this region and be used for restoration practices. nineteen hg-tolerant rhizobia were isolated from nodules of 11 legume species (of the genera medicago, trifolium, vicia, lupinus, phaseolus, and retama) and characterized. based on their growth on hg-supplemented media, the isolates w ...201222246528
the quorum sensing transcriptional regulator trar has separate binding sites for dna and the anti-activator.quorum sensing represents a mechanism by which bacteria control their genetic behaviors via diffusible signals that reflect their population density. trar, a quorum sensing transcriptional activator in the rhizobiaceae family, is regulated negatively by the anti-activator tram via formation of a trar-tram heterocomplex. prior structural analysis suggests that tram and dna bind to trar in distinct sites. here we combined isothermal titration calorimetry (itc) and electrophoretic mobility shift as ...201222274608
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