Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| elaboration of antibiofilm materials by chemical grafting of an antimicrobial peptide. | a peptide antibiotic, gramicidin a, was covalently bound to cystamine self-assembled monolayers on gold surfaces. each step of the surface functionalization was characterized by polarization modulation infrared reflection absorption spectroscopy and x-ray photoelectron spectroscopy. the antimicrobial activity of the anchored gramicidin was tested against three gram-positive bacteria (listeria ivanovii, enterococcus faecalis, and staphylococcus aureus), the gram-negative bacterium escherichia col ... | 2010 | 20949268 |
| food-borne enterococci integrate into oral biofilm: an in vivo study. | enterococci, particularly enterococcus faecalis, are still a primary concern in endodontic infections. to date, enterococci have been considered to be only transiently present in the oral cavity. the aim of this study was to examine whether different enterococci from food are able to reside in oral biofilm. | 2010 | 20951293 |
| ability of chemomechanical preparation with either rotary instruments or self-adjusting file to disinfect oval-shaped root canals. | oval-shaped root canals might represent a great challenge for proper disinfection. this study compared the capability of a newly developed instrument, the self-adjusting file (saf), and rotary nickel-titanium (niti) instrumentation to eliminate enterococcus faecalis populations from long oval root canals of extracted human teeth. as a secondary purpose, the ability of a modification in sampling technique to recover bacteria lodged in recesses of oval canals was evaluated. | 2010 | 20951301 |
| antimicrobial and heavy metal resistance in commensal enterococci isolated from pigs. | antibiotic resistance in animal isolates of enterococci is of public health concern because of the risk of transfer of antibiotic resistance isolates or resistance determinants to consumers via the food chain. in this study, phenotypic and genotypic resistance in 192 pig isolates of enterococci to ampicillin, avilamycin, avoparcin, bacitracin, flavophospholipol, gentamicin, narasin, tetracycline, tiamulin, tylosin, vancomycin, virginiamycin, copper and zinc were investigated by susceptibility te ... | 2010 | 20951513 |
| a multiresistance megaplasmid plg1 bearing a hylefm genomic island in hospital enterococcus faecium isolates. | enterococcus faecium is considered to be a nosocomial pathogen with increasing medical importance. the putative virulence factor, hyl(efm), encoding a putative hyaluronidase, is enriched among the hospital-associated polyclonal subpopulation of e. faecium.. the hyl(efm) gene is described to be part of a genomic island and was recently identified to be plasmid-located. here, we present a description of the structure, localization, and distribution of the putative pathogenicity factor hyl(efm) and ... | 2010 | 20951641 |
| antimicrobial effect of medical textiles containing bioactive fibres. | over the last few years, the textile industry has developed different methods for obtaining fabrics and fibres with an antimicrobial action for use in hospital environments and for other purposes. this study evaluates the antimicrobial action of bioactive(®)-treated fabric (btf), a commercially available textile containing silver for use in healthcare environments. unlike other biocides used in hospital fabrics, the prolonged use of silver has not been related to the appearance of resistant bact ... | 2010 | 20953889 |
| flow cytometry as a tool to determine the effects of cell wall-active antibiotics on vancomycin-susceptible and -resistant enterococcus faecalis strains. | flow cytometry and confocal microscopy were used to study the effects of vancomycin, daptomycin, telavancin, and pa1409, a new investigational vancomyquine, on the morphology, membrane potential, and permeability of glycopeptide-susceptible and -resistant enterococcus faecalis strains. daptomycin exerted the most pronounced effects on bacterial size and bacterial permeability against susceptible and resistant strains. | 2010 | 20956587 |
| enterococcus faecalis endocarditis severity in rabbits is reduced by igg fabs interfering with aggregation substance. | enterococcus faecalis is a significant cause of infective endocarditis, an infection of the heart endothelium leading to vegetation formation (microbes, fibrin, platelets, and host cells attached to underlying endothelial tissue). our previous research determined that enterococcal aggregation substance (as) is an important virulence factor in causation of endocarditis, although endocarditis may occur in the absence of as production. production of as by e. faecalis causes the organism to form agg ... | 2010 | 20957231 |
| chitosan-edta new combination is a promising candidate for treatment of bacterial and fungal infections. | chitosan is an attractive preparation widely used as a pharmaceutical excipient. this study aimed to evaluate the antimicrobial activities of chitosan derivatives, edta, and the newly developed chitosan-edta combination against gram-negative and gram-positive bacteria as well as candida albicans. antimicrobial activity was studied. both minimal inhibitory concentrations (mic) and minimal biocidal concentrations (mbc) were determined. chitosan acetic acid recorded lower mic values against enteroc ... | 2010 | 20963418 |
| antimicrobial activity of sodium hypochlorite-based irrigating solutions. | the objective of the present study was the in vitro evaluation of the antimicrobial activity of three different naocl-based endodontic irrigating solutions: a 5.25% conventional sodium hypochlorite solution; and two new irrigating solutions, a 5.25% sodium hypochlorite solution with the addition of a proteolytic enzyme and a surfactant; and a 5.25% sodium hypochlorite gel with inorganic silicate. | 2010 | 20963729 |
| validation of an extracted tooth model of endodontic irrigation. | an extracted tooth model of endodontic irrigation, incorporating reproducible inoculation and irrigation procedures, was tested against enterococcus faecalis using a variety of different irrigants in a latin square methodology. anova revealed no significant variations between the twelve teeth or experiments undertaken on different occasions; however, variation between irrigants was significant. | 2010 | 20971137 |
| myeloperoxidase selectively binds and selectively kills microbes. | myeloperoxidase (mpo) is reported to selectively bind to bacteria. the present study provides direct evidence of mpo binding selectivity and tests the relationship of selective binding to selective killing. the microbicidal effectiveness of h(2)o(2) and of ocl(-) was compared to that of mpo plus h(2)o(2). synergistic microbicidal action was investigated by combining streptococcus sanguinis, a h(2)o(2)-producing microbe showing low mpo binding, with high-mpo-binding escherichia coli, staphylococc ... | 2010 | 20974824 |
| influence of serum and necrotic soft tissue on the antimicrobial effects of intracanal medicaments. | the purpose of this study was to investigate the influence of serum and necrotic soft tissue on the antimicrobial activity of intracanal medicaments. the medicaments tested were: calcium hydroxyde/glycerin paste, calcium hydroxide/chlorhexidine paste, calcium hydroxide/camphorated paramonochlorophenol/glycerin paste, and chlorhexidine/zinc oxide paste. survival of enterococcus faecalis and candida albicans exposed to the medicaments tested in the presence or absence of serum or necrotic tissue w ... | 2010 | 20976377 |
| genotyping of enterococcus faecalis and enterococcus faecium isolates by use of a set of eight single nucleotide polymorphisms. | a single nucleotide polymorphism (snp) genotyping method for enterococcus faecalis and enterococcus faecium was developed using the "minimum snps" program. snp sets were interrogated using allele-specific real-time pcr. snp typing subdivided clonal complexes 2 and 9 of e. faecalis and 17 of e. faecium, members of which cause the majority of nosocomial infections globally. | 2010 | 20980566 |
| the response of lactobacillus casei and streptococcus faecalis to vitamin bc and vitamin bc conjugate. | 1946 | 20985636 | |
| a heat-labile stimulatory factor for streptococcus faecalis r. | 1946 | 20985651 | |
| the influence of a strepogenin concentrate on the metabolism of glutamic acid by streptococcus faecalis. | 1946 | 21001226 | |
| the amino acid requirements of streptococcus faecalis and the use of this organism for the determination of threonine in natural products. | 1946 | 21010894 | |
| the activity of lactobacillus casei factor, folic acid, and vitamin bc for streptococcus faecalis and lactobacillus casei. | 1946 | 21023647 | |
| construction and characterization of enterococcus faecalis cg110/gfp/pre25*, a tool for monitoring horizontal gene transfer in complex microbial ecosystems. | enterococci are among the most notorious bacteria involved in the spread of antibiotic resistance (abr) determinants via horizontal gene transfer, a process that leads to increased prevalence of antibiotic-resistant bacteria. in complex microbial communities with a high background of abr genes, detection of gene transfer is possible only when the abr determinant is marked. therefore, the conjugative multiresistance plasmid pre25, originating from a sausage-associated enterococcus faecalis, was t ... | 2010 | 21029153 |
| anti-bacterial activity of intermittent preventive treatment of malaria in pregnancy: comparative in vitro study of sulphadoxine-pyrimethamine, mefloquine, and azithromycin. | intermittent preventive treatment of malaria with sulphadoxine-pyrimethamine (sp) is recommended for the prevention of malaria in pregnancy in sub-saharan africa. increasing drug resistance necessitates the urgent evaluation of alternative drugs. currently, the most promising candidates in clinical development are mefloquine and azithromycin. besides the anti-malarial activity, sp is also a potent antibiotic and incurs significant anti-microbial activity when given as iptp - though systematic cl ... | 2010 | 21029476 |
| silver or nitrofurazone impregnation of urinary catheters has a minimal effect on uropathogen adherence. | bacterial adherence to the urinary catheter is an early step in biofilm formation and the pathogenesis of catheter associated urinary tract infection. we studied in vitro the effect of silver or nitrofurazone impregnation of urinary catheters on uropathogen ability to adhere to urinary catheters. | 2010 | 21030042 |
| non-aggregated ga(iii)-phthalocyanines in the photodynamic inactivation of planktonic and biofilm cultures of pathogenic microorganisms. | visible light-absorbing cationic water-soluble gallium(iii) phthalocyanines (gapcs) peripherally substituted with four and eight methylpyridyloxy groups were synthesized and investigated as antimicrobial photodynamic sensitizers. the inserted large gallium ion in the phthalocyanine ligand is axially substituted by one hydroxyl group which prevents aggregation of the complexes in aqueous solution. the cellular uptake and the photodynamic activity for the representative strains of the gram positiv ... | 2010 | 21031201 |
| growing insights into the safety of bacteriocins: the case of enterocin s37. | very few studies have been reported on the cytotoxicity and impact of bacteriocins, and especially enterocins, upon eukaryotic cells. in order to gain more information on the safety of bacteriocins, we focused this study on enterocin s37, a bacteriocin produced by enterococcus faecalis s37. we observed dose-dependent cytotoxicity toward undifferentiated caco-2/tc7 cells. moreover, no significant effect on differentiated monolayer caco-2/tc7 and no apoptotic features were observed when cells were ... | 2010 | 21035542 |
| antimicrobial resistance in swiss laying hens, prevalence and risk factors. | antimicrobial resistance is an emerging concern to public health, and food-producing animals are known to be a potential source for transmission of resistant bacteria to humans. as legislation of the european union requires to ban conventional cages for the housing of laying hens on the one hand, and a high food safety standard for eggs on the other hand, further investigations about the occurrence of antimicrobial resistance in alternative housing types are required. in this study, we determine ... | 2010 | 21040507 |
| a surveillance of high-level gentamicin-resistant enterococcal bacteremia. | enterococci have recently been recognized as a causative organism of intractable infections, including severe sepsis and infective endocarditis, in immunocompromised patients. this study investigated the epidemiological, microbiological, and prognostic characteristics of high-level gentamicin-resistant (hlgr) enterococcal bacteremia, including severe cases of infective endocarditis, in japan. a total of 155 enterococcal bacteremia episodes were identified between july 2007 and december 2009. hlg ... | 2010 | 21042826 |
| late-onset deep infectious keratitis after descemet stripping endothelial keratoplasty with vent incisions. | to report the clinical and histopathological findings of 3 cases of late-onset deep infectious keratitis after descemet stripping endothelial keratoplasty (dsek) with vent incisions. | 2011 | 21045667 |
| construction of mini-tn4001 transposon vector for mycoplasma gallisepticum. | the detailed genetic analysis of mycoplasmas has long been hampered by the lack of appropriate tools for genetic manipulation. in this study, the transposon vector, mini-tn4001tetm, was constructed containing the tnp gene, encoding a transposase gene in staphylococcus aureus, two copies of the is256 inverted repeat sequence (inner and outer) and the tetm gene, from the enterococcus faecalis tn916 transposon, conferring resistance to tetracycline. this vector was electro-transformed into mycoplas ... | 2010 | 21046326 |
| [antimicrobial potential of metarhizium taii in vitro]. | to investigate the antimicrobial potential of metarhizium tail and its corresponding active fractions. | 2010 | 21049622 |
| molecular characterization of glycopeptide-resistant enterococci from hospitals of the picardy region (france). | we studied 138 glycopeptide-resistant enterococci (gre) strains, consisting of 131 glycopeptide-resistant enterococcus faecium (grefm) and 7 glycopeptide-resistant enterococcus faecalis (grefs). the grefm strains were resistant to penicillin, ampicillin, vancomycin, and teicoplanin, while the grefs strains were only resistant to vancomycin and teicoplanin. the van a gene was the only glycopeptide determinant present in all gre isolates investigated. genes coding for hyl and hyl+ esp were detecte ... | 2010 | 21052490 |
| enterococcus faecalis complicating dermal filler injection: a case of virulent facial abscesses. | 2010 | 21053422 | |
| sealing ability of mta-angelus with propyleneglycol in furcal perforations. | this study evaluated the sealing ability of gray mta-angelus mixed with propyleneglycol in furcal perforations using a bacterial leakage test. furcal perforations were created in 30 human mandibular molars using a size 3 round bur the samples were divided randomly into 2 experimental groups (n=10) according to the mixing agent. in g1, the mta powder was mixed with propyleneglycol, while distilled water was used in g2. a 3:1 powder-liquid ratio was used for both groups. the mta was placed in the ... | 2010 | 21053685 |
| assessment of antimicrobial susceptibility of enterococcus faecalis isolated from chronic periodontitis in biofilm versus planktonic phase. | enterococci are often associated with chronic and recurrent infectious diseases because of their antimicrobial resistance. the aim of this study is to assess antimicrobial susceptibility of enterococcus faecalis in chronic periodontitis. | 2010 | 21054225 |
| multidrug-resistant enterococci lack crispr-cas. | clustered, regularly interspaced short palindromic repeats (crispr) provide bacteria and archaea with sequence-specific, acquired defense against plasmids and phage. because mobile elements constitute up to 25% of the genome of multidrug-resistant (mdr) enterococci, it was of interest to examine the codistribution of crispr and acquired antibiotic resistance in enterococcal lineages. a database was built from 16 enterococcus faecalis draft genome sequences to identify commonalities and polymorph ... | 2010 | 21060735 |
| on the origin of reactive oxygen species and antioxidative mechanisms in enterococcus faecalis. | enterococci cause serious infections due to a number of virulence factors and wide-spread antibiotic resistance. a molecular mechanism involved in the pathogenesis of enterococcal infections is oxidative stress. enterococcus faecalis produces a variety of antioxidative enzymes involved in the oxidative stress response, a process that is regulated by several transcriptional regulators. in addition, direct production of free radicals derived from oxygen has been proved and hypothesized, respective ... | 2010 | 21062535 |
| tetracycline resistance associated with commensal bacteria from representative ready-to-consume deli and restaurant foods. | proper knowledge of antibiotic resistance (ar) dissemination is essential for effective mitigation. this study examined the profiles of tetracycline-resistant (tetr) commensal bacteria from representative ready-to-consume food samples from salad bars at local grocery stores and restaurants. out of 900 tetr isolates examined, 158 (17.6%) carried one or more of tetm, tetl, tets, and tetk genes by conventional pcr, 28 harbored more than one tetr determinants. the most prevalent genotype was tetm, w ... | 2010 | 21067672 |
| nucleic acids of enterococcus faecalis strain ec-12 are potent toll-like receptor 7 and 9 ligands inducing interleukin-12 production from murine splenocytes and murine macrophage cell line j774.1. | in experiment 1 of this study, the interleukin-12 (il-12)-inducing ability of six enterococcus strains was evaluated in comparison with that of five lactobacillus strains using murine splenocytes. at the same time, the involvement of toll-like receptor (tlr) ligands in il-12-inducing ability was assessed using splenocytes from tlr2-, tlr4- and myd88-deficient mice. most enterococcus strains, especially enterococcus faecalis strain ec-12, exerted higher il-12-inducing ability compared with the la ... | 2010 | 21073545 |
| antimicrobial characterisation of solithromycin (cem-101), a novel fluoroketolide: activity against staphylococci and enterococci. | solithromycin (cem-101) is a novel fluoroketolide with high potency against gram-positive and gram-negative bacteria commonly associated with community-acquired respiratory tract infections and skin and skin-structure infections. in this study, solithromycin and comparator antimicrobials were tested against a contemporary collection of staphylococcus aureus, coagulase-negative staphylococci, enterococcus faecalis, enterococcus faecium and other enterococcus spp. collected in the sentry antimicro ... | 2011 | 21075602 |
| inhibition of efflux pumps in methicillin-resistant staphylococcus aureus and enterococcus faecalis resistant strains by triterpenoids from momordica balsamina. | six cucurbitane-type triterpenoids (1-6) isolated from the aerial parts of momordica balsamina were evaluated for their ability to inhibit the activity of bacterial efflux pumps of methicillin-resistant staphylococcus aureus (mrsa) col(oxa), enterococcus faecalis atcc 29212, salmonella enterica subsp. i serovar typhimurium 5408 and s. typhimurium 5408cip strains. the latter strain overproduces the acrb transporter of the acrab-tolc efflux pump six-fold compared with its parent. compounds 4-6 wer ... | 2010 | 21075604 |
| tangential-flow ultrafiltration with integrated inhibition detection for recovery of surrogates and human pathogens from large-volume source water and finished drinking water. | tangential-flow ultrafiltration was optimized for the recovery of escherichia coli, enterococcus faecalis, clostridium perfringens spores, bacteriophages ms2 and prd1, murine norovirus, and poliovirus seeded into 100-liter surface water (sw) and drinking water (dw) samples. sw and dw collected from two drinking water treatment plants were then evaluated for human enteric viruses. | 2010 | 21075885 |
| the occurrence of virulence traits among high-level aminoglycosides resistant enterococcus isolates obtained from feces of humans, animals, and birds in south korea. | enterococcus isolates (1500) obtained from the feces of 48 humans, 209 domesticated food animals, and 155 wild geese in south korea were characterized with respect to species status by pcr analyses and resistance to antibiotics. of the 1500 strains examined, the majority (n=577) were enterococcus faecalis from 224 (54.4%) of the samples feces, while 299 were of e. faecium from 125 of the samples (30.3%), 224 were e. hirae from 101 (24.5%) of the samples, 94 were e. casseliflavus from 43 (10.4%) ... | 2010 | 21078531 |
| pharmacodynamics of telavancin studied in an in vitro pharmacokinetic model of infection. | the antibacterial effects of telavancin, vancomycin, and teicoplanin against six staphylococcus aureus strains (1 methicillin-susceptible s. aureus [mssa] strain, 4 methicillin-resistant s. aureus [mrsa] strains, and 1 vancomycin-intermediate s. aureus [visa] strain) and three enterococcus sp. strains (1 enterococcus faecalis strain, 1 enterococcus faecium strain, and 1 vancomycin-resistant e. faecium [vref] strain) were compared using an in vitro pharmacokinetic model of infection. analyzing th ... | 2010 | 21078943 |
| characterization of macrolide resistance genes in haemophilus influenzae isolated from children with cystic fibrosis. | to determine the mechanism(s) of macrolide resistance in haemophilus influenzae isolated from cystic fibrosis (cf) patients participating in a randomized placebo-controlled trial of azithromycin. | 2010 | 21081549 |
| evaluation of the antimicrobial effect of super-oxidized water (sterilox®) and sodium hypochlorite against enterococcus faecalis in a bovine root canal model. | ideally root canal irrigants should have, amongst other properties, antimicrobial action associated with a lack of toxicity against periapical tissues. sodium hypochlorite (naocl) is a widely used root canal irrigant, however it has been shown to have a cytotoxic effect on vital tissue and therefore it is prudent to investigate alternative irrigants. sterilox's aquatine alpha electrolyte® belongs to the group of the super-oxidized waters; it consists of a mixture of oxidizing substances, and has ... | 2010 | 21085808 |
| in vitro antibacterial activity of doripenem against clinical isolates from french teaching hospitals: proposition of zone diameter breakpoints. | the aims of the study were to determine the in vitro activity of doripenem, a new carbapenem, against a large number of bacterial pathogens and to propose zone diameter breakpoints for clinical categorization in france according to the european committee on antimicrobial susceptibility testing (eucast) minimum inhibitory concentration (mic) breakpoints. the mics of doripenem were determined by the broth microdilution method against 1,547 clinical isolates from eight french hospitals. the disk di ... | 2010 | 21088861 |
| serology of strains of streptococcus faecalis which produce hyaluronidase. | 1966 | 21090479 | |
| grafted glycopolymer-based receptor mimics on polymer support for selective adhesion of bacteria. | a sugar-containing monomer (2-lactobionamidoethyl methacrylate, lama) was grafted on a polypropylene (pp) microfiltration membrane surface by uv-induced graft copolymerization. the degree of grafting can be controlled by variation of monomer concentration, uv irradiation time, and photoinitiator concentration. fourier transform infrared spectroscopy and scanning electron microscopy were employed to confirm the surface modification on the membranes. the water contact angle was used to evaluate th ... | 2010 | 21090578 |
| functional cationic nanomagnet-porphyrin hybrids for the photoinactivation of microorganisms. | cationic nanomagnet-porphyrin hybrids were synthesized and their photodynamic therapy capabilities were investigated against the gram (-) escherichia coli bacteria, the gram (+) enterococcus faecalis bacteria and t4-like phage. the synthesis, structural characterization, photophysical properties, and antimicrobial activity of these new materials are discussed. the results show that these new multicharged nanomagnet-porphyrin hybrids are very stable in water and highly effective in the photoinact ... | 2010 | 21090642 |
| distribution of selected virulence genes and antibiotic resistance in enterococcus species isolated from the south nation river drainage basin, ontario, canada. | isolate and characterize water enterococci from the south nation river drainage basin, an area dominated by agriculture. | 2010 | 21091592 |
| [a clinical analysis of 25 cases of prosthetic valve endocarditis]. | to report the clinical characteristics of prosthetic valve endocarditis (pve). | 2010 | 21092446 |
| prevalence, phenotype, and genotype of enterococcus faecalis isolated from saliva and root canals in patients with persistent apical periodontitis. | the aim of this study was to investigate the prevalence, phenotype, and genotype of enterococcus faecalis isolated from saliva and root canals in patients with endodontic treatment failure. | 2010 | 21092811 |
| [bacterial meningitis secondary to spinal analgesia and anaesthesia]. | although rare, infectious complications from spinal analgesia and anaesthesia (sa) can have serious morbidity and mortality. this study describes the clinical features and outcome of sa-associated bacterial meningitis in adults seen in a hospital over a 25 yearperiod. | 2010 | 21093704 |
| characterization of three lactic acid bacteria and their isogenic ldh deletion mutants shows optimization for yatp (cell mass produced per mole of atp) at their physiological phs. | several lactic acid bacteria use homolactic acid fermentation for generation of atp. here we studied the role of the lactate dehydrogenase enzyme on the general physiology of the three homolactic acid bacteria lactococcus lactis, enterococcus faecalis, and streptococcus pyogenes. of note, deletion of the ldh genes hardly affected the growth rate in chemically defined medium under microaerophilic conditions. however, the growth rate was affected in rich medium. furthermore, deletion of ldh affect ... | 2010 | 21097579 |
| characterization of lytic enzyme open reading frame 9 (orf9) derived from enterococcus faecalis bacteriophage phief24c. | in bacteriophage (phage) therapy against gram-positive bacteria, such as staphylococcus aureus, listeria monocytogenes, and enterococcus faecalis, members of a genus of spo1-like viruses are typically employed because of their extreme virulence and broad host spectrum. phage φef24c, which is a spo1-like virus infecting e. faecalis, has previously been characterized as a therapeutic phage candidate. in addition to the phage itself, phage endolysin is also recognized as an effective antimicrobial ... | 2010 | 21097580 |
| diversity of the fsr-gele region of the enterococcus faecalis genome but conservation in strains with partial deletions of the fsr operon. | most enterococcus faecalis isolates carry gele, but many are gelatinase nonproducers due to the lack of fsrc (ef_1820) to ef_1841 (fsrc-ef_1841; 23.9 kb in strain v583), including most of the locus encoding fsr, which activates gele expression. analysis of 22 accessible e. faecalis genomes revealed the identity of the 53-amino-acid propeptide of fsrd across multiple mlsts (multilocus sequence types), although 12 distinctly different variations were found in the ef_1814-to-ef_1902 region. diversi ... | 2010 | 21097591 |
| the in vitro contribution of autolysins to bacterial killing elicited by amoxicillin increases with inoculum size in enterococcus faecalis. | the mechanisms of antibiotic-induced cell death are poorly understood despite the critical role of the bactericidal activities of antibiotics for successful treatment of severe infections. these mechanisms include irreversible damaging of macromolecules by reactive oxygen species and bacteriolysis mediated by peptidoglycan hydrolases (autolysins). we have assessed the contribution of the second mechanism by using an autolysin-deficient mutant of enterococcus faecalis and shown that it contribute ... | 2010 | 21098238 |
| outcomes of therapy: vancomycin-resistant enterococcal bacteremia in hematology and bone marrow transplant patients. | purpose: the purpose of this study was to evaluate the risk factors associated with the treatment failure and 30-day mortality in hematology and bone marrow transplant patients treated with daptomycin or linezolid for vancomycin-resistant enterococci (vre) bacteremia. the safety and tolerability of therapy was also assessed. methods: this single-center, retrospective study included adult patients admitted to the hematology or bone marrow transplant service with documented vancomycin-resistant en ... | 2010 | 21110047 |
| characterization of virulence factors and clonal diversity of enterococcus faecalis isolates from treated dental root canals. | the high prevalence of enterococcus faecalis in root canal treated teeth with post-treatment disease, as evidenced by both molecular and traditional culturing methods, suggests that this species may be a key player in endodontic treatment failure. this study aimed to detect virulence factors by phenotypic and western blotting tests, and virulence genes by pcr from 20 clinical strains of e. faecalis isolated from treated root canals of teeth with (10) or without (10) apical periodontitis. moreove ... | 2010 | 21111042 |
| synthesis and antibacterial activity study of a novel class of cationic anthraquinone analogs. | reported previously by our group, one-pot cycloaddition using naphthoquinone, sodium azide and alkyl halides can lead to the formation of both 1-alkyl-1h- and 2-alkyl-2h-naphtho[2,3-d]triazole-4,9-diones. herein, the effect of leaving group and additive in dictating the selectivity between the formation of 1-alkyl-1h- and 2-alkyl-2h-naphtho[2,3-d]triazole-4,9-diones has been further investigated. in the process of investigating the factors that control the selectivity and the biological activity ... | 2010 | 21111625 |
| co(iii), ni(ii), zn(ii) and cd(ii) complexes with 2-acetyl-2-thiazoline thiosemicarbazone: synthesis, characterization, x-ray structures and antibacterial activity. | the new ligand 2-acetyl-2-thiazoline thiosemicarbazone (hattsc) and the complexes [co(attsc)(2)](2)[cocl(4)]·2h(2)o (1), [co(attsc)(2)]no(3)·h(2)o (2), [ni(hattsc)(2)](no(3))(2) (3), [zncl(2)(hattsc)]·ch(3)cn (4), [{cdcl(hattsc)}(2)(μ-cl)(2)]·2h(2)o (5) and [{cd(no(3))(hattsc)}(2)(μ-no(3))(2)] (6) were isolated and characterized by a variety of physico-chemical techniques and x-ray diffraction. the structure of hattsc in 1 and 2 presented a thiolate form while in 3-6 the thione form was present, ... | 2010 | 21112129 |
| persistence of dead-cell bacterial dna in ex vivo root canals and influence of nucleases on dna decay in vitro. | the fate of dna from bacteria that do not survive in the root canal is uncertain, yet dna longevity may confound recovery of authentic etiologic participants in the disease process. this study assessed the recovery of pcr-detectable dna in ex vivo human root canals and some environmental factors on the decay of microbial dna. | 2010 | 21112536 |
| [is the iron an essential nutrient for enterococci?]. | enterococci were considered as not requiring iron. the aim of study was evaluation of relationship between enterococci and iron. this study examined these relationships in a 71 strains belonging to two species--enterococcus faecalis and enterococcus faecium, which are often isolated from human infections. the iron is an essential nutrient for enterococci. demonstrated that iron--regardless of the concentration in the medium--is collected during growth. iron deficiency in the nutrient medium resu ... | 2010 | 21114020 |
| outcome of patients with ischemic-like cholangiopathy with secondary sclerosing cholangitis after liver transplantation. | sclerosing cholangitis in critically ill patients (sc-cip) with sepsis and acute respiratory distress syndrome (ards) is a cholestatic liver disease with a rapid progression to liver cirrhosis and hepatic failure. data on outcome of these patients after liver transplantation (lt) are sparse. | 2010 | 21114429 |
| ceftaroline activity against pathogens associated with complicated skin and skin structure infections: results from an international surveillance study. | to assess the spectrum and potency of ceftaroline, a novel anti-methicillin-resistant staphylococcal cephalosporin, against a 2008 surveillance collection of clinical isolates from patients in the usa and europe. | 2010 | 21115451 |
| substantiation in enterococcus faecalis of dose-dependent resistance and cross-resistance to pore-forming antimicrobial peptides by use of a polydiacetylene-based colorimetric assay. | a better understanding of the antimicrobial peptide (amp) resistance mechanisms of bacteria will facilitate the design of effective and potent amps. therefore, to understand resistance mechanisms and for in vitro assessment, variants of enterococcus faecalis that are resistant to different doses of the fungal amp alamethicin (alm(r)) were selected and characterized. the resistance developed was dose dependent, as both doses of alamethicin and degrees of resistance were colinear. the formation of ... | 2010 | 21115699 |
| in vitro antibacterial activity of e-101 solution, a novel myeloperoxidase-mediated antimicrobial, against gram-positive and gram-negative pathogens. | e-101 solution (e-101) is a novel myeloperoxidase-mediated antimicrobial. it is composed of porcine myeloperoxidase (pmpo), glucose oxidase, glucose as the substrate and specific amino acids in an aqueous vehicle. e-101 is being developed for topical application directly into surgical wounds to prevent surgical site infections (ssis). the in vitro activity of e-101 was investigated. | 2010 | 21118915 |
| telavancin activity against gram-positive bacteria isolated from patients with skin and skin-structure infections. | telavancin is approved in the united states and canada for the treatment of complicated skin and skin structure infections (csssi) in adults caused by susceptible gram-positive organisms. the antimicrobial activity of telavancin and comparators was evaluated against 5,027 (2007-2008) gram-positive bacteria responsible for sssi in medical centers in asia-pacific, european, latin american, and north american regions. telavancin was active against staphylococcus aureus (mic₅₀(/)₉₀, 0.12/0.25 mg/l; ... | 2010 | 21123152 |
| expression of linear permutated variants from circular enterocin as-48. | to confirm whether the head-to-tail circularization could be involved in the stability and activity of the circular bacteriocin as-48, two permutated linear structural as-48a genes have been constructed by circular permutation. the absence of the leaderless linear as(23/24) and as(48/49) proteins in escherichia coli, under all the conditions investigated, supports the idea that the circular backbone is important to stabilize their structure and also indicates the significance of a leader peptide ... | 2010 | 21130135 |
| host range of enterococcal vana plasmids among gram-positive intestinal bacteria. | the most prevalent type of acquired glycopeptide resistance is encoded by the vana transposon tn1546 located mainly on transferable plasmids in enterococcus faecium. the limited occurrence in other species could be due to the lack of inter-species transferability and/or stability of tn1546-containing plasmids in other species. we investigated the in vitro transferability of 14 pre-characterized vana-containing plasmids hosted by e. faecium (n = 9), enterococcus faecalis (n = 4) and enterococcus ... | 2010 | 21131318 |
| antimicrobial resistance of escherichia coli and enterococcus faecalis in housed laying-hen flocks in europe. | summarythe aim of this study was to determine the potential association between housing type and multiple drug resistance (mdr) in escherichia coli and enterococcus faecalis isolates recovered from 283 laying-hen flocks. in each flock, a cloacal swab from four hens was collected and produced 1102 e. coli and 792 e. faecalis isolates. broth microdilution was used to test susceptibility to antimicrobials. country and housing type interacted differently with the mdr levels of both species. in the e ... | 2010 | 21134321 |
| antibacterial activity of the essential oil from rosmarinus officinalis and its major components against oral pathogens. | the essential oil of rosmarinus officinalis l. (rosemary) was obtained by hydro-distillation and analysed by gas chromatography-mass spectrometry. sixty-two constituents were identified, representing 98.06% of the total oil content. oxygenated monoterpenes were the predominant components. the rosemary oil was characterized as having prominent (> 5%) contents of camphor (18.9%), verbenone (11.3%), a-pinene (9.6%), beta-myrcene (8.6%), 1,8-cineole (8.0%), and beta-caryophyllene (5.1%). the antimic ... | 2010 | 21138060 |
| infected kaposi's sarcoma. | 2010 | 21139313 | |
| antimicrobial resistance in community-acquired urinary tract infections: results from the korean antimicrobial resistance monitoring system. | the korean antimicrobial resistance monitoring system was established and the first nationwide surveillance of bacterial uropathogens was conducted during the period from january 2008 to june 2009. with the cooperation of 34 medical centers throughout south korea, a total of 1994 strains belonging to clinically relevant bacterial uropathogens were collected from patients with community-acquired urinary tract infections (utis). to compare with past data, understand a trend of antimicrobial resist ... | 2010 | 21140281 |
| the sam-responsive s(mk) box is a reversible riboswitch. | the s(mk) (sam-iii) box is an s-adenosylmethionine (sam)-responsive riboswitch found in the 5' untranslated region of metk genes, encoding sam synthetase, in many members of the lactobacillales. sam binding causes a structural rearrangement in the rna that sequesters the shine-dalgarno (sd) sequence by pairing with a complementary anti-sd (asd) sequence; sequestration of the sd sequence inhibits binding of the 30s ribosomal subunit and prevents translation initiation. we observed a slight increa ... | 2010 | 21143313 |
| chitosan-cobalt(ii) and nickel(ii) chelates as antibacterial agents. | antibacterial behavior of chitosan-bivalent metal chelates (co and ni) was investigated in vitro against standard bacteria, staphylococcus aureus atcc 4533, s. faecalis atcc 8043 and escherichia coli atcc 25923. the chitosan-metal chelates were prepared by varying the molar ratio of metal ions to a fixed amount of chitosan. the metal ion contents, structural properties and morphology of the chelates were respectively determined using icp-oes, ft-ir and sem. all the chitosan-metal chelates showed ... | 2010 | 21145344 |
| resazurin metabolism assay for root canal disinfectant evaluation on dual-species biofilms. | endodontic infections are caused by polymicrobial biofilms. therefore, novel root canal disinfectants should be evaluated not only on single-species biofilms but also on dual- or mixed-species biofilms. a simple, high-throughput assay is urgently needed for this. in this study, the application of the resazurin metabolism assay was investigated for the evaluation of a root canal disinfectant on dual-species biofilms. | 2010 | 21146072 |
| involvement, and dissemination, of the enterococcal small multidrug resistance transporter qacz in resistance to quaternary ammonium compounds. | to investigate the role of a putative small multidrug resistance transporter, annotated in enterococcus faecalis v583 genome as efa0010 (we will refer to this gene as qacz), in decreased susceptibility to biocides. | 2010 | 21147826 |
| clpb, a class iii heat-shock gene regulated by ctsr, is involved in thermotolerance and virulence of enterococcus faecalis. | here, we transcriptionally and phenotypically characterized the clpb gene from enterococcus faecalis. northern blot analysis identified a monocistronic mrna strongly induced at 48 and 50 °c. in silico analysis identified that the clpb gene encodes a protein of 868 aa with a predicted molecular mass of approximately 98 kda, presenting two conserved atp-binding domains. sequence analysis also identified a ctsr-binding box upstream of the putative -10 sequence, and inactivation of the ctsr gene res ... | 2010 | 21148206 |
| multilocus sequence typing of enterococcus faecalis isolates demonstrating different lesion types in broiler breeders. | a total of 69 isolates of enterococcus faecalis from broiler breeders demonstrating different lesion types and representing eight different flocks were characterized by multilocus sequence typing. twenty isolates obtained from healthy birds representing two additional flocks were included for comparison. a total of 12 different sequence types (sts) was obtained. correlation between st and lesion type was not demonstrated. however, three sts (st82, st174, st177) made up 81% of the isolates associ ... | 2010 | 21154051 |
| enterococcus faecalis infection in root canals - host-derived or exogenous source? | enterococcus faecalis is associated with a significant number of refractory endodontic infections. previous studies report a prevalence of ent. faecalis ranging from 24% up to 77% in teeth with failed endodontic treatment. the origin of the micro-organism remains unclear, as enterococci do not belong to the normal oral microflora. the aim of this study was to determine whether these enterococci were of endogenous or exogenous origin. | 2010 | 21155997 |
| effects of demethylfruticuline a and fruticuline a from salvia corrugata vahl. on biofilm production in vitro by multiresistant strains of staphylococcus aureus, staphylococcus epidermidis and enterococcus faecalis. | in this study, demethylfruticuline a (dfa) and fruticuline a (fa), two quinones representing the major diterpenoid components of the exudate produced by the aerial parts of salvia corrugata, were assessed for their ability to modify surface characteristics, such as hydrophobicity, and to inhibit synthesis of biofilm in vitro by multiresistant staphylococcus aureus, staphylococcus epidermidis and enterococcus faecalis. five strains of s. aureus (three meticillin-resistant and two meticillin-susce ... | 2010 | 21163629 |
| cholera- and anthrax-like toxins are among several new adp-ribosyltransferases. | chelt, a cholera-like toxin from vibrio cholerae, and certhrax, an anthrax-like toxin from bacillus cereus, are among six new bacterial protein toxins we identified and characterized using in silico and cell-based techniques. we also uncovered medically relevant toxins from mycobacterium avium and enterococcus faecalis. we found agriculturally relevant toxins in photorhabdus luminescens and vibrio splendidus. these toxins belong to the adp-ribosyltransferase family that has conserved structure d ... | 2010 | 21170356 |
| the nationwide study of bacterial pathogens associated with urinary tract infections conducted by the japanese society of chemotherapy. | this study was conducted by the japanese society of chemotherapy and is the first nationwide study on bacterial pathogens isolated from patients with urinary tract infections at 28 hospitals throughout japan between january 2008 and june 2008. a total of 688 bacterial strains were isolated from adult patients with urinary tract infections. the strains investigated in this study are as follows: enterococcus faecalis (n = 140), escherichia coli (n = 255), klebsiella pneumoniae (n = 93), proteus mi ... | 2010 | 21174142 |
| insights into the biofilm lifestyle of enterococci. | 2010 | 21178446 | |
| [analysis of relationship between the detection of enterococcus faecalis in post-treatment endodontic disease and clinical symptom or sign]. | to detect the enterococcus faecalis (e. faecalis) in post-treatment endodontic disease, and to analyze the relationship between the occurrence of e. faecalis and clinical symptom. | 2010 | 21179693 |
| [chlorhexidine as a root canal irrigant--antimicrobial and scanning electron microscopic evaluation]. | selection of irrigant is very important for long-term success of root canal therapy. | 2010 | 21180084 |
| inhibitory activity of root canal irrigants against candida albicans, enterococcus faecalis and staphylococcus aureus. | the present study evaluated the antimicrobial activity of three root canal irrigants against enterococcus faecalis, candida albicans, and staphylococcus aureus. these microorganisms were incubated in the presence of citric acid (6 and 10%), edta (17%), and naocl (0.5, 1.0, 2.5, and 5.25%). agar diffusion tests were performed and redox indicator resazurin was used to evaluate the inhibitory effect of the irrigants on the metabolic activity of these microorganisms. the mean diameters of the inhibi ... | 2010 | 21180960 |
| an in vitro study of antibacterial effect of calcium hydroxide and chlorhexidine on enterococcus faecalis. | to evaluate and compare the antibacterial effects of chlorhexidine and calcium hydroxide on enterococcus faecalis. | 2010 | 21187615 |
| patent ductus arteriosus with infective endarteritis. | 2010 | 21189697 | |
| residual antibacterial activity of a new modified sodium hypochlorite-based endodontic irrigation solution. | in this in vitro study the antibacterial substantivity of a new sodium hypochlorite-based root canal irrigant (hypoclean) in bovine root dentin was investigated. | 2011 | 21196865 |
| hemolysis and infective endocarditis in a mitral prosthetic valve. | traumatic intravascular hemolysis after heart valve replacement can be a serious problem. it is commonly associated with either structural deterioration or paravalvular leaks. a 63-year-old woman with a six-year history of surgery for mitral stenosis presented with complaints of weakness and dyspnea. she received treatment at other centers three times in the past six months for dyspnea and anemia requiring transfusion of red blood cells. transthoracic echocardiography showed a normally functioni ... | 2010 | 21200125 |
| the annotated complete dna sequence of enterococcus faecalis bacteriophage φef11 and its comparison with all available phage and predicted prophage genomes. | φef11 is a temperate siphoviridae bacteriophage isolated by induction from a lysogenic enterococcus faecalis strain. the φef11 dna was completely sequenced and found to be 42,822 bp in length, with a g+c mol% of 34.4%. genome analysis revealed 65 orfs, accounting for 92.8% of the dna content. all except for seven of the orfs displayed sequence similarities to previously characterized proteins. the genes were arranged in functional modules, organized similar to that of several other phages of low ... | 2011 | 21204936 |
| comparative genomic analysis reveals significant enrichment of mobile genetic elements and genes encoding surface structure-proteins in hospital-associated clonal complex 2 enterococcus faecalis. | enterococci rank among the leading causes of nosocomial infections. the failure to identify pathogen-specific genes in enterococcus faecalis has led to a hypothesis where the virulence of different strains may be linked to strain-specific genes, and where the combined endeavor of the different gene-sets result in the ability to cause infection. population structure studies by multilocus sequence typing have defined distinct clonal complexes (cc) of e. faecalis enriched in hospitalized patients ( ... | 2011 | 21205308 |
| acceleration of enterococcus faecalis biofilm formation by aggregation substance expression in an ex vivo model of cardiac valve colonization. | infectious endocarditis involves formation of a microbial biofilm in vivo. enterococcus faecalis aggregation substance (asc10) protein enhances the severity of experimental endocarditis, where it has been implicated in formation of large vegetations and in microbial persistence during infection. in the current study, we developed an ex vivo porcine heart valve adherence model to study the initial interactions between asc10(+) and asc10(-)e. faecalis and valve tissue, and to examine formation of ... | 2010 | 21209892 |
| characterization and transfer of antibiotic resistance in lactic acid bacteria from fermented food products. | the study provides phenotypic and molecular analyses of the antibiotic resistance in lactic acid bacteria (lab) from fermented foods in xi'an, china. lab strains (n = 84) belonging to 16 species of lactobacillus (n = 73), and streptococcus thermophilus (n = 11) were isolated and identified by sequencing their 16s rrna gene. all strains were susceptible to ampicillin, bacitracin, and cefsulodin, and intrinsically resistant to nalidixic acid, kanamycin, and vancomycin (except l. bulgaricus, l. aci ... | 2011 | 21212956 |
| virulence factors in enterococci from partridges (alectoris rufa) representing a food safety problem. | forty-three enterococcus isolates recovered from fecal samples of partridges, during the partridge hunting season, were studied for gelatinase and β-hemolysis activities. the presence of fsr-gele genes and the cyl operon was studied by polymerase chain reaction and correlated with gelatinase and β-hemolysis production, respectively. in addition, genes encoding additional virulence factors (cpd, hyl, agg, esp, and ace) was also analyzed in all enterococci. most of our enterococcus faecalis isolat ... | 2011 | 21214387 |
| synthesis of sugars embodying conjugated carbonyl systems and related triazole derivatives from carboxymethyl glycoside lactones. evaluation of their antimicrobial activity and toxicity. | the synthesis of a series of pyranoid derivatives comprising a conjugated carbonyl function and related triazole derivatives, structurally suitable for bioactivity evaluation, was achieved in few steps starting from readily available carboxymethyl glycoside lactones (cmgl). 3-enopyranosid-2-uloses were generated by oxidation/elimination of tri-o-acylated 2-hydroxy pyranosides. subsequent wittig olefination provided stereoselectively 2-c-branched-chain conjugated dienepyranosides with (e)-configu ... | 2010 | 21215645 |
| a comparative evaluation of 3 root canal filling systems. | this in vitro study was conducted to evaluate and compare the microbial leakage of a new obturation system: guttaflow with resin-based resilon cones and epiphany sealer, keeping gutta-percha with ah plus sealer as the standard. | 2011 | 21216635 |
| endodontic microbiology. | root canal therapy has been practiced ever since 1928 and the success rate has tremendously increased over the years owing to various advancements in the field. one main reason is the complete understanding of the microbiology involved in the endodontic pathology. this has helped us to modify the conventional treatment plans and effectively combat the microorganisms. now, studies are aiming to explore the characteristics of the "most" resistant organism and the methods to eliminate them. this ar ... | 2010 | 21217951 |
| a comparative evaluation of antimicrobial efficacy and flow properties for epiphany, guttaflow and ah-plus sealer. | to test the antimicrobial efficacy and flow properties of guttaflow, epiphany sealer and ah-plus sealer. | 2011 | 21219358 |