Publications

virus-induced gene silencing as a tool to study tomato fruit biochemistry.	virus-induced gene silencing (vigs) is an excellent reverse genetic tool for the study of gene function in plants, based on virus infection. in this chapter, we describe a high-throughput approach based on vigs for the study of tomato fruit biochemistry. it comprises the selection of the sequence for silencing using bioinformatics tools, the cloning of the fragment in the tobacco rattle virus (trv), and the agroinfiltration of tomato fruits mediated by agrobacterium tumefaciens.	2016	26577782
the stress-related, rhizobial small rna rcsr1 destabilizes the autoinducer synthase encoding mrna sini in sinorhizobium meliloti.	quorum sensing is a cell density-dependent communication system of bacteria relying on autoinducer molecules. during the analysis of the post-transcriptional regulation of quorum sensing in the nitrogen fixing plant symbiont sinorhizobium meliloti, we predicted and verified a direct interaction between the 5'-utr of sini mrna encoding the autoinducer synthase and a small rna (srna), which we named rcsr1. in vitro, rcsr1 prevented cleavage in the 5'-utr of sini by rnase e and impaired sini transl ...	2015	26588798
development of an efficient in vitro plant regeneration system amenable to agrobacterium- mediated transformation of a recalcitrant grain legume blackgram (vigna mungo l. hepper).	an efficient, rapid and direct multiple shoot regeneration system amenable to agrobacterium-mediated transformation from primary leaf with intact petiole of blackgram (vigna mungo) is established for the first time. the effect of the explant type and its age, type and concentration of cytokinin and auxin either alone or in combination and genotype on multiple shoot regeneration efficiency and frequency was optimized. the primary leaf explants with petiole excised from 4-day-old seedlings directl ...	2015	26600677
multigene engineering in rice using high-capacity agrobacterium tumefaciens bibac vectors.	the high-capacity binary bacterial artificial chromosome (bibac) vector system permits the insertion of large fragments of dna, up to 150 kb, into plants via agrobacterium-mediated transformation. here, we describe an optimized protocol for transformation of japonica rice (oryza sativa l.) using this system. calli derived from mature embryos are transformed using agrobacterium strain lba4404 that carries the bibac vector and the super-virulent helper plasmid pch32. transformed calli are then reg ...	2016	26614279
transient protein expression by agroinfiltration in lettuce.	current systems of recombinant protein production include bacterial, insect, and mammalian cell culture. however, these platforms are expensive to build and operate at commercial scales and/or have limited abilities to produce complex proteins. in recent years, plant-based expression systems have become top candidates for the production of recombinant proteins as they are highly scalable, robust, safe, and can produce complex proteins due to having a eukaryotic endomembrane system. newly develop ...	2016	26614281
detection of quorum sensing molecules and biofilm formation in ralstonia solanacearum.	many bacteria use small diffusible signaling molecules to communicate each other termed as quorum sensing (qs). most gram-negative bacteria use acyl homoserine lactone (ahl) as qs signal molecules. using these signaling molecules, bacteria are able to express specific genes in response to population density. this work aimed to detect the production of qs signal molecules and biofilm formation in ralstonia solanacearum isolated from various diseased tomato plants with symptoms of bacterial wilt. ...	2016	26620535
polyclonal antibodies for specific detection of tobacco host cell proteins can be efficiently generated following rubisco depletion and the removal of endotoxins.	the production of biopharmaceutical proteins in plants requires efficient downstream processing steps that remove impurities such as host cell proteins (hcps) and adventitious endotoxins produced by bacteria during transient expression. we therefore strived to develop effective routines for endotoxin removal from plant extracts and the subsequent use of the extracts to generate antibodies detecting a broad set of hcps. at first, we depleted the superabundant protein ribulose-1,5-bisphosphate car ...	2016	26632519
quantum chemical modeling of the dehalogenation reaction of haloalcohol dehalogenase.	the dehalogenation reaction of haloalcohol dehalogenase hhec from agrobacterium radiobacter ad1 was investigated theoretically using hybrid density functional theory methods. hhec catalyzes the enantioselective conversion of halohydrins into their corresponding epoxides. the reaction is proposed to be mediated by a catalytic ser132-tyr145-arg149 triad, and a distinct halide binding site is suggested to facilitate halide displacement by stabilizing the free ion. we investigated the hhec-mediated ...	2008	26636366
involvement of agrobacterium tumefaciens galacturonate tripartite atp-independent periplasmic (trap) transporter gaapqm in virulence gene expression.	monosaccharides capable of serving as nutrients for the soil bacterium agrobacterium tumefaciens are also inducers of the vir regulon present in the tumor-inducing (ti) plasmid of this plant pathogen. one such monosaccharide is galacturonate, the predominant monomer of pectin found in plant cell walls. this ligand is recognized by the periplasmic sugar binding protein chve, which interacts with the vira histidine kinase that controls vir gene expression. although chve is also a member of the chv ...	2016	26637603
an efficient protocol for agrobacterium-mediated transformation of the biofuel plant jatropha curcas by optimizing kanamycin concentration and duration of delayed selection.	jatropha curcas is considered a potential biodiesel feedstock crop. currently, the value of j. curcas is limited because its seed yield is generally low. transgenic modification is a promising approach to improve the seed yield of j. curcas. although agrobacterium-mediated genetic transformation of j. curcas has been pursued for several years, the transformation efficiency remains unsatisfying. therefore, a highly efficient and simple agrobacterium-mediated genetic transformation method for j. c ...	2017	26640597
adaptation of the agrobacterium tumefaciens virg response regulator to activate transcription in plants.	the agrobacterium tumefaciens virg response regulator of the vira/virg two-component system was adapted to function in tobacco protoplasts. the subcellular localization of virg and vira proteins transiently expressed in onion cells was determined using gfp fusions. preliminary studies using gal4dbd-vp16 fusions with virg and escherichia coli uhpa, and narl response regulators indicated compatibility of these bacterial proteins with the eukaryotic transcriptional apparatus. a strong transcription ...	2016	26646288
decreased expression of 14-3-3 in paracoccidioides brasiliensis confirms its involvement in fungal pathogenesis.	the interaction between the fungal pathogen paracoccidioides brasiliensis and host cells is usually mediated by specific binding events between adhesins on the fungal surface and receptors on the host extracellular matrix or cell surface. one molecule implicated in the p. brasiliensis-host interaction is the 14-3-3 protein. the 14-3-3 protein belongs to a family of conserved regulatory molecules that are expressed in all eukaryotic cells and are involved in diverse cellular functions. here, we i ...	2016	26646480
[agrobacterium tumefaciens-mediated transformation of aureobasidium pullulans and high-efficient screening for polymalic acid producing strain].	to develop a genetic transformation method of aureobasidium pullulans and t-dna insertion for high-efficient screening of polymalic acid (pma) producing strain. agrobacterium tumefaciens-agl1, containing the selection genes encoding hygromycin b phosphotase or phosphinothricin acetyltranferase, was used to transform aureobasidium pullulans cctcc m2012223 and transformants were confirmed by colony pcr method. transferred dna (t-dna) insertional mutants were cultured in microwell plate, and screen ...	2015	26647581
cloning and evaluation of different constitutive promoters in the oleaginous yeast rhodosporidium toruloides.	the oleaginous yeast rhodosporidium toruloides is an unconventional yeast species that can accumulate a high content of lipids. because it belongs to the basidiomycetous group of fungus, limited tools and functional elements are available for genetic engineering of r. toruloides and related red yeasts. here we report the functional evaluation of five constitutive promoters from this yeast. we assembled a reporter gene expression cassette, consisting of a promoter, the hygromycin gene (hyg) and t ...	2016	26648086
constitutive expression of the tzs gene from agrobacterium tumefaciens virg mutant strains is responsible for improved transgenic plant regeneration in cotton meristem transformation.	key message : virg mutant strains of a nopaline type of agrobacterium tumefaciens increase the transformation frequency in cotton meristem transformation. constitutive cytokinin expression from the tzs gene in the virg mutant strains is responsible for the improvement. strains of agrobacterium tumefaciens were tested for their ability to improve cotton meristem transformation frequency. two disarmed a. tumefaciens nopaline strains with either a virgn54d constitutively active mutation or virgi77v ...	2016	26650837
community analysis of root- and tuber-associated bacteria in field-grown potato plants harboring different resistance levels against common scab.	eight genotypes of potato plants with different resistance levels against common scab were grown in a field infested with streptomyces turgidiscabies. dna was extracted from the roots, tubers, and rhizosphere soils of each of the eight genotypes at the flowering stage, and the quantity of s. turgidiscabies genomic dna was assessed by real-time pcr using a taqman probe. the results obtained showed that the different potato genotypes had significant impacts on the population levels of s. turgidisc ...	2015	26657303
advances in agrobacterium tumefaciens-mediated genetic transformation of graminaceous crops.	steady increase in global population poses several challenges to plant science research, including demand for increased crop productivity, grain yield, nutritional quality and improved tolerance to different environmental factors. transgene-based approaches are promising to address these challenges by transferring potential candidate genes to host organisms through different strategies. agrobacterium-mediated gene transfer is one such strategy which is well known for enabling efficient gene tran ...	2016	26660352
the essential role of spermidine in growth of agrobacterium tumefaciens is determined by the 1,3-diaminopropane moiety.	the ubiquitous polyamine spermidine is indispensable for eukaryotic growth and cell proliferation. a conserved vital function of spermidine across eukaryotes is conferred by its aminobutyl group that is transferred to a single lysine in translation factor eif5a to form the essential hypusine post-translational modification required for cellular translation. in direct contrast, although spermidine is absolutely essential for growth of α-proteobacterial plant pathogen agrobacterium tumefaciens, we ...	2016	26682642
agrobacterium tumefaciens-mediated transformation: an efficient tool for insertional mutagenesis and targeted gene disruption in harpophora oryzae.	the endophytic filamentous fungus harpophora oryzae is a beneficial endosymbiont isolated from the wild rice. h. oryzae could not only effectively improve growth rate and biomass yield of rice crops, but also induce systemic resistance against the rice blast fungus, magnaporthe oryzae. in this study, agrobacterium tumefaciens-mediated transformation (atmt) was employed and optimized to modify the h. oryzae genes by either random dna fragment integration or targeted gene replacement. our results ...	2016	26686612
interaction of antifungal peptide bp15 with stemphylium vesicarium, the causal agent of brown spot of pear.	peptide bp15 has shown antifungal activity against several plant pathogenic fungi, including stemphylium vesicarium, the causal agent of brown spot of pear. bp15 inhibits the germination, growth and sporulation of s. vesicarium and displays post-infection activity by stopping fungal infection in pear leaves. in this work, live-cell imaging was undertaken to understand the antifungal mechanism of bp15. a double-staining method based on the combination of calcofluor white and sytox green coupled w ...	2016	26693685
transient expression of p-type atpases in tobacco epidermal cells.	transient expression in tobacco cells is a convenient method for several purposes such as analysis of protein-protein interactions and the subcellular localization of plant proteins. a suspension of agrobacterium tumefaciens cells carrying the plasmid of interest is injected into the intracellular space between leaf epidermal cells, which results in dna transfer from the bacteria to the plant and expression of the corresponding proteins. by injecting mixes of agrobacterium strains, this system o ...	2016	26695049
expression of agrobacterium homolog genes encoding t-complex recruiting protein under virulence induction conditions.	the proteins encoded by three agrobacterial genes, atu5117, atu4860, and atu4856, are highly homologous to each other in amino acid sequence. all three proteins can bind to vird2 and are named vbp1, vbp2, and vbp3 (vird2-binding protein), respectively. vbp is involved in t-dna transfer by recruiting the t-complex from the cytosol to the polar transport apparatus t4ss (type iv secretion system) and is defined as the "t-complex recruiting protein." however, it remains unknown how these three homol ...	2015	26696988
[preliminary analyses on bacterial diversity and resistance in infection-related skin disorders].	to explore the bacterial diversity and resistance in infection-related skin disorders.	2015	26704163
[identification of a pigment-polyketide synthase gene deleted mutant of monascus ruber m7].	to reveal the function of a polyketide synthase gene (pkspt), probably responsible for the synthesis of monascus pigments in monascus ruber m7.	2015	26710605
discrete responses to limitation for iron and manganese in agrobacterium tumefaciens: influence on attachment and biofilm formation.	transition metals such as iron and manganese are crucial trace nutrients for the growth of most bacteria, functioning as catalytic cofactors for many essential enzymes. dedicated uptake and regulatory systems have evolved to ensure their acquisition for growth, while preventing toxicity. transcriptomic analysis of the iron- and manganese-responsive regulons of agrobacterium tumefaciens revealed that there are discrete regulatory networks that respond to changes in iron and manganese levels. comp ...	2016	26712936
the plant gaba signaling downregulates horizontal transfer of the agrobacterium tumefaciens virulence plasmid.	in the tumor-inducing (ti) agrobacterium tumefaciens, quorum sensing activates the horizontal transfer of the virulent ti plasmid. in pure culture, this process can be impaired by the a. tumefaciens blcc lactonase, whose expression is induced by gamma-aminobutyrate (gaba). it was therefore hypothesized that host gaba content might modulate quorum sensing and virulence gene dissemination during a. tumefaciens infection. we examined gaba metabolism and transport in arabidopsis thaliana tumors comb ...	2016	26714842
nicotine dehydrogenase complexed with 6-hydroxypseudooxynicotine oxidase involved in the hybrid nicotine-degrading pathway in agrobacterium tumefaciens s33.	nicotine, a major toxic alkaloid in tobacco wastes, is degraded by bacteria, mainly via pyridine and pyrrolidine pathways. previously, we discovered a new hybrid of the pyridine and pyrrolidine pathways in agrobacterium tumefaciens s33 and characterized its key enzyme 6-hydroxy-3-succinoylpyridine (hsp) hydroxylase. here, we purified the nicotine dehydrogenase initializing the nicotine degradation from the strain and found that it forms a complex with a novel 6-hydroxypseudooxynicotine oxidase. ...	2016	26729714
effects of a petunia scaffold/matrix attachment region on copy number dependency and stability of transgene expression in nicotiana tabacum.	transgenes in genetically modified plants are often not reliably expressed during development or in subsequent generations. transcriptional gene silencing (tgs) as well as post-transcriptional gene silencing (ptgs) have been shown to occur in transgenic plants depending on integration pattern, copy number and integration site. in an effort to reduce position effects, to prevent read-through transcription and to provide a more accessible chromatin structure, a p35s-ß-glucuronidase (p35s-gus) tran ...	2016	26732611
antibacterial and antitumour activities of some plants grown in turkey.	screening of antibacterial and antitumour activities of 33 different extracts prepared with three types of solvents (water, ethanol and methanol) was conducted. the extracts were obtained from 11 different plant species grown in turkey: eryngium campestre l., alchemilla mollis (buser) rothm., dorycnium pentaphyllum scop., coronilla varia l., onobrychis oxyodonta boiss., fritillaria pontica wahlenb., asarum europaeum l., rhinanthus angustifolius c. c. gmelin, doronicum orientale hoffm., campanula ...	2014	26740759
in vitro screening for anti-acetylcholiesterase, anti-oxidant, anti-glucosidase, anti-inflammatory and anti-bacterial effect of three traditional medicinal plants.	in this study we investigated the phytoconstituents calluna vulgaris, ferula hermonis and tribulus terrestris, and then assessed their possible biological activities by using standard methods. a preliminary phytochemical investigation of the three extracts revealed the presence of alkaloids, flavonoids, proteins, lipids, phenolic compounds, saponins, sterols and amino acids. three extracts showed anti-oxidant effect as they inhibited the 1,1-diphenyl-2-picryl hydrazyl (dpph) oxidation and produc ...	2014	26740784
efficient construction of large genomic deletion in agrobacterium tumefaciens by combination of cre/loxp system and triple recombineering.	in order to develop an efficient system for deleting genomic segment in agrobacterium tumefaciens to analyze gene functions and construct marker gene-free recombinant strains, a cre recombinase expression plasmid was constructed by placing its encoding gene under the control of ptet promoter and cloning into the plasmid replicable in both a. tumefaciens and e. coli. triple recombineering was applied to efficiently construct integrative vectors which were used to introduce loxp sites and selectio ...	2016	26742770
improvement in agrobacterium-mediated transformation of chickpea (cicer arietinum l.) by the inhibition of polyphenolics released during wounding of cotyledonary node explants.	agrobacterium-mediated transformation of chickpea (cicer arietinum l.) has been performed using cotyledonary node explants (cns), which release phenolics upon excision that are detrimental to the viability of agrobacterium tumefaciens and result in low transformation frequency. twelve low molecular weight phenolic compounds and salicylic acid were identified in the exudates released upon excision during the preparation of cotyledonary nodes by reverse phase high-performance liquid chromatography ...	2017	26747430
purification, crystallization and structural elucidation of d-galactaro-1,4-lactone cycloisomerase from agrobacterium tumefaciens involved in pectin degradation.	pectin is found in the cell wall of plants and is often discarded as waste. a number of research groups are interested in redirecting this biomass waste stream for the production of fuel and bulk chemicals. the primary monomeric subunit of this polysaccharide is d-galacturonate, a six-carbon acid sugar that is degraded in a five-step pathway to central metabolic intermediates by some bacteria, including agrobacterium tumefaciens. in the third step of the pathway, d-galactaro-1,4-lactone is conve ...	2016	26750482
succinic semialdehyde promotes prosurvival capability of agrobacterium tumefaciens.	succinic semialdehyde (ssa), an important metabolite of γ-aminobutyric acid (gaba), is a ligand of the repressor attj regulating the expression of the attj-attklm gene cluster in the plant pathogen agrobacterium tumefaciens. while the response of a. tumefaciens to gaba and the function of attklm have been extensively studied, genetic and physiological responses of a. tumefaciens to ssa remain unknown. in combination with microarray and genetic approaches, this study sets out to explore new roles ...	2016	26755630
peritonitis due to rhizobium radiobacter.	rhizobium radiobacter (agrobacterium radiobacter) is an aerobic gram-negative rod belonging to agrobacterium genus, a group of phytopathogenic bacteria present in the soil that has been implicated in human opportunistic infections. we report a clinical case of bacterial peritonitis in a 5-year-old child with chronic renal disease in peritoneal dialysis, who had a history of direct soil contact identified. the infection was treated with ceftazidime and piperaciline+tazobactam without relapses or ...	2011	26761111
the receiver of the agrobacterium tumefaciens vira histidine kinase forms a stable interaction with virg to activate virulence gene expression.	the plant pathogen agrobacterium tumefaciens carries a virulence gene system that is required for the initiation of crown gall tumors on susceptible plants. expression of the vir genes is activated by the vira/virg two component regulatory system. vira is a histidine kinase which signals the presence of certain chemicals found at the site of a plant wound. the receiver domain located at its carboxyl terminus defines vira as a hybrid histidine kinase. here, we show that the vira receiver interact ...	2015	26779177
application of a scalable plant transient gene expression platform for malaria vaccine development.	despite decades of intensive research efforts there is currently no vaccine that provides sustained sterile immunity against malaria. in this context, a large number of targets from the different stages of the plasmodium falciparum life cycle have been evaluated as vaccine candidates. none of these candidates has fulfilled expectations, and as long as we lack a single target that induces strain-transcending protective immune responses, combining key antigens from different life cycle stages seem ...	2015	26779197
an improved single-step cloning strategy simplifies the agrobacterium tumefaciens-mediated transformation (atmt)-based gene-disruption method for verticillium dahliae.	the soilborne fungal pathogen verticillium dahliae infects a broad range of plant species to cause severe diseases. the availability of verticillium genome sequences has provided opportunities for large-scale investigations of individual gene function in verticillium strains using agrobacterium tumefaciens-mediated transformation (atmt)-based gene-disruption strategies. traditional atmt vectors require multiple cloning steps and elaborate characterization procedures to achieve successful gene re ...	2016	26780432
biodegradation of the cross-linked copolymer of acrylamide and potassium acrylate by soil bacteria.	chemical cross-linking and the high molecular weight of superabsorbent copolymers (saps) are the two main causes of their resistance to biodegradation. however, sap particles are colonized by microorganisms. for the purposes of this study, the dry technical copolymer of acrylamide and potassium acrylate containing 5.28 % of unpolymerized monomers was wrapped in a geotextile and incubated in unsterile haplic luvisol soil as a water absorbing geocomposite. the highest number of soil bacteria that ...	2016	26817471
comparative characterization of three bacterial exo-type alginate lyases.	alginate, a major acidic polysaccharide in brown macroalgae, has attracted attention as a carbon source for production of ethanol and other chemical compounds. alginate is monomerized by exo-type alginate lyase into an unsaturated uronate; thus, this enzyme is critical for the saccharification and utilization of alginate. although several exo-type alginate lyases have been characterized independently, their activities were not assayed under the same conditions or using the same unit definition, ...	2016	26827758
tatc-dependent translocation of pyoverdine is responsible for the microbial growth suppression.	infections are often not caused by a colonization of pseudomonas aeruginosa alone but by a consortium of other bacteria. little is known about the impact of p. aeruginosa on the growth of other bacteria upon coinfection. here, cell-ree culture supernatants obtained from p. aeruginosa suppressed the growth of a number of bacterial strains such as corynebacterium glutamicum, bacillus subtilis, staphylococcus aureus, and agrobacterium tumefaciens, but had little effect on the growth of escherichia ...	2016	26832668
arm-in-arm response regulator dimers promote intermolecular signal transduction.	bacteriophytochrome photoreceptors (bphps) and their cognate response regulators make up two-component signal transduction systems which direct bacteria to mount phenotypic responses to changes in environmental light quality. most of these systems utilize single-domain response regulators to transduce signals through unknown pathways and mechanisms. here we describe the photocycle and autophosphorylation kinetics of rtbphp1, a red light-regulated histidine kinase from the desert bacterium ramlib ...	2016	26833410
the opposite roles of agda and glaa on citric acid production in aspergillus niger.	citric acid is produced by an industrial-scale process of fermentation using aspergillus niger as a microbial cell factory. however, citric acid production was hindered by the non-fermentable isomaltose and insufficient saccharification ability in a. niger when liquefied corn starch was used as a raw material. in this study, a. niger tna 101δagda was constructed by deletion of the α-glucosidase-encoding agda gene in a. niger cgmcc 10142 genome using agrobacterium tumefaciens-mediated transformat ...	2016	26837219
identification and characterization of an anti-oxidative stress-associated mutant of aspergillus fumigatus transformed by agrobacterium tumefaciens.	aspergillus fumigatus is one of the most common opportunistic pathogenic fungi, surviving in various environmental conditions. maintenance of the redox homeostasis of the fungus relies upon the well‑organized regulation between reactive oxygen species generated by immune cells or its own organelles, and the activated anti‑oxidative stress mechanism. to investigate such a mechanism, the present study obtained a number of randomly‑inserted mutants of a. fumigatus, mediated by agrobacterium tumefac ...	2016	26847000
the fungal-specific transcription factor vdpf influences conidia production, melanized microsclerotia formation and pathogenicity in verticillium dahliae.	verticillium dahliae is a soil-borne, hemibiotrophic phytopathogenic fungus that causes wilting in crop plants. here, we constructed a random insertional mutant library using agrobacterium tumefaciens-mediated transformation to study the pathogenicity and regulatory mechanisms of v. dahliae. the fungal-specific transcription factor-encoding gene vdpf was shown to be associated with vegetative growth and virulence, with the highest transcript expression occurring during conidia formation in the v ...	2016	26857810
construction of artificial mirnas to prevent drought stress in solanum tuberosum.	the use of artificial micrornas (amirnas) is still a relatively new technique in molecular biology with a wide range of applications in life sciences. here, we describe the silencing of the cbp80/abh1 gene in solanum tuberosum with the use of amirna. the cbp80/abh1 protein is part of the cap binding complex (cbc), which is involved in plant responses to drought stress conditions. transformed plants with a decreased level of cbp80/abh1 display increased tolerance to water shortage conditions. we ...	2016	26867630
antisense expression of gossypium hirsutum udp-glucuronate decarboxylase in arabidopsis leads to changes in cell wall components.	udp-glucuronate decarboxylase (udp-xylose synthase; uxs, ec 4.1.1.35) is an essential enzyme of the non-cellulosic polysaccharide biosynthetic pathway. in the present study, using transient expression of fluorescently labeled gossypium hirsutum uxs (ghuxs3) protein in onion epidermal cells, we observed that this protein was distributed in the cytoplasm. the ghuxs3 cdna of cotton was expressed in an antisense orientation in arabidopsis thaliana by agrobacterium tumefaciens-mediated transformation ...	2016	26909959
effects of feeding transgenic corn with mcry1ac or maroacc gene to laying hens for 12 weeks on growth, egg quality and organ health.	the objective of the present study was to investigate the effect of feeding two transgenic corn lines containing the mcry1ac gene from bacillus thuringiensis strain (bt-799) and the maroacc gene from agrobacterium tumefaciens strain (cc-2), respectively, on growth, egg quality and organ health indicators. expression of the mcry1ac gene confers resistance to pyrausta nubilalis and the maroacc gene confers tolerance to herbicides. healthy hens (n=96 placed in cages; 3 hens/cage) were randomly assi ...	2016	26915544
quorum sensing inhibitory potential and molecular docking studies of sesquiterpene lactones from vernonia blumeoides.	the increasing incidence of multidrug-resistant gram-negative bacterial pathogens has focused research on the suppression of bacterial virulence via quorum sensing inhibition strategies, rather than the conventional antimicrobial approach. the anti-virulence potential of eudesmanolide sesquiterpene lactones previously isolated from vernonia blumeoides was assessed by inhibition of quorum sensing and in silico molecular docking. inhibition of quorum sensing-controlled violacein production in chro ...	2016	26920717
the addition of n-hexanoyl-homoserine lactone to improve the microbial flocculant production of agrobacterium tumefaciens strain f2, an exopolysaccharide bioflocculant-producing bacterium.	in this study, n-hexanoyl-homoserine lactone (c6-hsl), a member of the n-acyl-homoserine lactone class of microbial quorum sensing (qs) signaling molecules, was used to improve microbial flocculant production. after exogenous c6-hsl was added, exopolysaccharide concentration of microbial flocculants was improved by 1.6-fold and flocculation rate of microbial flocculants was increased by 10 %. fermentation conditions with added c6-hsl were further optimized through response surface methodology. t ...	2016	26922725
identifying a carotenoid cleavage dioxygenase 4a gene and its efficient agrobacterium-mediated genetic transformation in bixa orellana l.	carotenoids are metabolized to apocarotenoids through the pathway catalysed by carotenoid cleavage oxygenases (ccos). the apocarotenoids are economically important as it is known to have therapeutic as well as industrial applications. for instance, bixin from bixa orellana and crocin from crocus sativus are commercially used as a food colourant and cosmetics since prehistoric time. in our present study, ccd4a gene has been identified and isolated from leaves of b. orellana for the first time and ...	2016	26922728
the mettl20 homologue from agrobacterium tumefaciens is a dual specificity protein-lysine methyltransferase that targets ribosomal protein l7/l12 and the β subunit of electron transfer flavoprotein (etfβ).	human mettl20 is a mitochondrial, lysine-specific methyltransferase that methylates the β-subunit of electron transfer flavoprotein (etfβ). interestingly, putative mettl20 orthologues are found in a subset of α-proteobacteria, including agrobacterium tumefaciens using an activity-based approach, we identified in bacterial extracts two substrates of recombinant mettl20 from a. tumefaciens (atmettl20), namely etfβ and the ribosomal protein rpl7/l12. we show that atmettl20, analogous to the human e ...	2016	26929405
spike-dip transformation of setaria viridis.	traditional method of agrobacterium-mediated transformation through the generation of tissue culture had limited success for setaria viridis, an emerging c4 monocot model. here we present an efficient in planta method for agrobacterium-mediated genetic transformation of s. viridis using spike dip. pre-anthesis developing spikes were dipped into a solution of agrobacterium tumefaciens strain agl1 harboring the β-glucuronidase (gus) reporter gene driven by the cauliflower mosaic virus 35s (camv35s ...	2016	26932666
monitoring the efficacy of mutated allium sativum leaf lectin in transgenic rice against rhizoctonia solani.	rice sheath blight, caused by rhizoctonia solani is one of the most devastating diseases of rice. it is associated with significant reduction in rice productivity worldwide. a mutant variant of mannose binding allium sativum leaf agglutinin (masal) was previously reported to exhibit strong antifungal activity against r. solani. in this study, the masal gene has been evaluated for its in planta antifungal activity in rice plants.	2016	26932667
identification of the non-ribosomal peptide synthetase responsible for biosynthesis of the potential anti-cancer drug sansalvamide in fusarium solani.	sansalvamide is a cyclic pentadepsipeptide produced by fusarium solani and has shown promising results as potential anti-cancer drug. the biosynthetic pathway has until now remained unidentified, but here we used an agrobacterium tumefaciens-mediated transformation (atmt) approach to generate knockout mutants of two candidate non-ribosomal peptide synthetases (nrps29 and nrps30). comparative studies of secondary metabolites in the two deletion mutants and wild type confirmed the absence of sansa ...	2016	26936154
rhizobium radiobacter: a recently recognized cause of bacterial keratitis.	to present the first reported cases of keratitis caused by rhizobium radiobacter.	2016	26938327
salivary proteins of spider mites suppress defenses in nicotiana benthamiana and promote mite reproduction.	spider mites (tetranychidae sp.) are widely occurring arthropod pests on cultivated plants. feeding by the two-spotted spider mite t. urticae, a generalist herbivore, induces a defense response in plants that mainly depends on the phytohormones jasmonic acid and salicylic acid (sa). on tomato (solanum lycopersicum), however, certain genotypes of t. urticae and the specialist species t. evansi were found to suppress these defenses. this phenomenon occurs downstream of phytohormone accumulation vi ...	2016	26946468
tobacco against ebola virus disease.	the ebola virus disease (evd), formerly known as a hemorrhagic fever and discovered in 1976, is dangerous, highly infectious disease with very high mortality. there are no licensed therapeutics against evd, although a range of medicines and therapies are currently being evaluated. during the 2014 ebola outbreak, an experimental drug named zmapp was administered on an emergency basis to seven patients of which five were recovered. currently, since february 2015, zmapp is tested in clinical trials ...	2015	26946569
double-stranded rna-mediated interference of dumpy genes in bursaphelenchus xylophilus by feeding on filamentous fungal transformants.	rna interference (rnai) is a valuable tool for studying gene function in vivo and provides a functional genomics platform in a wide variety of organisms. the pinewood nematode, bursaphelenchus xylophilus, is a prominent invasive plant-parasitic nematode and has become a serious worldwide threat to forest ecosystems. presently, the complete genome sequence of b. xylophilus has been published, and research involving genome-wide functional analyses is likely to increase. in this study, we describe ...	2016	26953254
co-overexpression of brassica juncea npr1 (bjnpr1) and trigonella foenum-graecum defensin (tfgd) in transgenic peanut provides comprehensive but varied protection against aspergillus flavus and cercospora arachidicola.	coexpression of two antifungal genes ( npr1 and defensin ) in transgenic peanut results in the development of resistance to two major fungal pathogens, aspergillus flavus and cercospora arachidicola. fungal diseases have been one of the principal causes of crop losses with no exception to peanut (arachis hypogeae l.), a major oilseed crop in asia and africa. to address this problem, breeding for fungal disease resistance has been successful to some extent against specific pathogens. however, com ...	2016	26956134
improvement of soybean transformation via agrobacterium tumefaciens methods involving α-aminooxyacetic acid and sonication treatments enlightened by gene expression profile analysis.	antagonists and sonication treatment relieved the structural barriers of agrobacterium entering into cells; hindered signal perception and transmission; alleviated defense responses and increased cell susceptibility to agrobacterium infection. soybean gene expression analysis was performed to elucidate the general response of soybean plant to agrobacterium at an early stage of infection. agrobacterium infection stimulated the pamps-triggered immunity (bri1, bak1, bzr1, fls2 and efr) and effector ...	2016	26960402
development of a microemulsion formulation for antimicrobial seca inhibitors.	in our previous study, we have identified five antimicrobial small molecules via structure based design, which inhibit seca of candidatus liberibacter asiaticus (las). seca is a critical protein translocase atpase subunit and is involved in pre-protein translocation across and integration into the cellular membrane in bacteria. in this study, eleven compounds were identified using similarity search method based on the five lead seca inhibitors identified previously. the identified seca inhibitor ...	2016	26963811
antitumor and anti-angiogenic potentials of isolated crude saponins and various fractions of rumex hastatus d. don.	cancer, being the foremost challenge of the modern era and the focus of world-class investigators, gargantuan research is in progress worldwide to explore novel therapeutic for its management. the exploitation of natural sources has been proven to be an excellent approach to treat or minify the excessive angiogenesis and proliferation of cells. similarly, based the ethnomedicinal uses and literature survey, the current study is designed to explore the anti-tumor and anti-angiogenic potentials of ...	2016	26969307
the helicobacter pylori cag pathogenicity island protein cag1 is associated with the function of t4ss.	the human pathogen helicobacter pylori is involved in gastric diseases ranging from gastritis to gastric cancer. virulent strains harboring the cag pathogenicity island (cag pai) which encode a type iv secretion system (t4ss) can induce pro-inflammatory cytokines such as interleukin-8 and deliver their major effector proteins caga into the gastric cells. while a subset of cag pai genes have been identified to be the homologues of t4ss genes from agrobacterium tumefaciens, a majority have unknown ...	2016	26971262
protocol: transient expression system for functional genomics in the tropical tree theobroma cacao l.	theobroma cacao l., the source of cocoa, is a crop of significant economic value around the world. to facilitate the study of gene function in cacao we have developed a rapid agrobacterium-mediated transient genetic transformation protocol. here we present a detailed methodology for our transformation assay, as well as an assay for inoculation of cacao leaves with pathogens.	2016	26973706
rhamnose synthase activity is required for pathogenicity of the vascular wilt fungus verticillium dahliae.	the initial interaction of a pathogenic fungus with its host is complex and involves numerous metabolic pathways and regulatory proteins. considerable attention has been devoted to proteins that play a crucial role in these interactions, with an emphasis on so-called effector molecules that are secreted by the invading microbe to establish the symbiosis. however, the contribution of other types of molecules, such as glycans, is less well appreciated. here, we present a random genetic screen that ...	2017	26996832
production of a novel bioflocculant and its flocculation performance in aluminum removal.	a novel bioflocculant cbf with high flocculating activity, produced by mixed culture of rhizobium radiobacter f2 and bacillus sphaericus f6 from soil, was investigated with regard to its production and flocculation performance in al(iii) removal. the most preferred carbon source, nitrogen source and c/n ratio (w/w) for strains f2 and f6 to produce cbf were glucose, urea and 20, respectively. the optimal inoculum size for cbf production was 10 % (v/v). the optimal initial ph, culture temperature ...	2016	27002778
groe chaperonins assisted functional expression of bacterial enzymes in saccharomyces cerevisiae.	rapid advances in the capabilities of reading and writing dna along with increasing understanding of microbial metabolism at the systems-level have paved an incredible path for metabolic engineering. despite these advances, post-translational tools facilitating functional expression of heterologous enzymes in model hosts have not been developed well. some bacterial enzymes, such as escherichia coli xylose isomerase (xi) and arabinose isomerase (ai) which are essential for utilizing cellulosic su ...	2016	27003667
using the β-glucosidase catalyzed reaction product glucose to improve the ionic liquid tolerance of β-glucosidases.	pretreating biomass with ionic liquids (il) increases enzyme accessibility and cellulose is typically recovered through precipitation with an anti-solvent. an industrially feasible pretreatment and hydrolysis process requires robust cellulases that are stable and active in the presence of either small amounts of ils co-precipitated with recovered cellulose or for saccharifications in the presence of il. β-glucosidase (bg) hydrolyzes cellobiose into two molecules of glucose (glc) and is the last ...	2016	27006691
agrobacterium-mediated stable genetic transformation of populus angustifolia and populus balsamifera.	the present study demonstrates agrobacterium tumefaciens-mediated stable genetic transformation of two species of poplar - populus angustifolia and populus balsamifera. the binary vector pcambia-npro-long-luc containing the luciferase reporter gene was used to transform stem internode and axillary bud explants. putative transformants were regenerated on selection-free medium using our previously established in vitro regeneration method. explant type, genotype, effect of pre-culture, agrobacteriu ...	2016	27014319
environment control to improve recombinant protein yields in plants based on agrobacterium-mediated transient gene expression.	agrobacterium-mediated transient expression systems enable plants to produce a wide range of recombinant proteins on a rapid timescale. to achieve economically feasible upstream production and downstream processing, two yield parameters should be considered: (1) recombinant protein content per unit biomass and (2) recombinant protein productivity per unit area-time at the end of the upstream production. because environmental factors in the upstream production have impacts on these parameters, en ...	2016	27014686
rnai-mediated resistance against cotton leaf curl disease in elite indian cotton (gossypium hirsutum) cultivar narasimha.	cotton leaf curl disease (clcud) is caused by several distinct begomovirus species in association with disease-specific betasatellite essential for induction of disease symptoms. clcud is a serious threat for the cultivation of cotton (gossypium sp.) and several species in the family malvaceae. in this study, rnai-based approach was applied to generate transgenic cotton (gossypium hirsutum) plants resistant to cotton leaf curl rajasthan virus (clcurv). an intron hairpin (ihp) rnai construct capa ...	2016	27028623
agrobacterium-mediated transformation in the green alga haematococcus pluvialis (chlorophyceae, volvocales)(1).	the first successful agrobacterium-mediated transformation of the green alga haematococcus pluvialis flot. using the binary vectors hosting the genes coding for gus (β-glucuronidase), gfp (green fluorescent protein), and hpt (hygromycin phosphotransferase) is reported here. colonies resistant to hygromycin at 10 mg · l(-1) expressed β-glucuronidase. the greenish yellow fluorescence of gfp was observed when the hygromycin-resistant cells were viewed with a fluorescent microscope. pcr was used to ...	2009	27034041
regulatory activities of four arsr proteins in agrobacterium tumefaciens 5a.	arsr is a well-studied transcriptional repressor that regulates microbe-arsenic interactions. most microorganisms have an arsr gene, but in cases where multiple copies exist, the respective roles or potential functional overlap have not been explored. we examined the repressors encoded by arsr1 and arsr2 (ars1 operon) and by arsr3 and arsr4 (ars2 operon) in agrobacterium tumefaciens 5a. arsr1 and arsr4 are very similar in their primary sequences and diverge phylogenetically from arsr2 and arsr3, ...	2016	27037117
production and characterization of recombinant human acid α-glucosidase in transgenic rice cell suspension culture.	pompe disease is a fatal genetic muscle disorder caused by a deficiency of acid α-glucosidase (gaa), a glycogen-degrading lysosomal enzyme. in this study, the human gaa cdna gene was synthesized from human placenta cells and cloned into a plant expression vector under the control of the rice α-amylase 3d (ramy3d) promoter. the plant expression vector was introduced into rice calli (oryza sativa l. cv. dongjin) mediated by agrobacterium tumefaciens. genomic dna pcr and northern blot analysis were ...	2016	27050503
biochemical and biocatalytic characterization of 17 novel halohydrin dehalogenases.	halohydrin dehalogenases are rare but catalytically remarkable enzymes since they are able to form novel c-c, c-o, c-n, or c-s bonds. very recently, a motif-based sequence database mining approach resulted in the identification of 37 novel halohydrin dehalogenase enzymes, many of them exhibiting only low sequence similarity to previously known halohydrin dehalogenases. in an attempt to explore the biocatalytic potential of these newly identified enzymes, 17 representatives from all six phylogene ...	2016	27052376
development of marker-free transgenic lettuce resistant to mirafiori lettuce big-vein virus.	lettuce big-vein disease caused by mirafiori lettuce big-vein virus (mlbvv) is found in major lettuce production areas worldwide, but highly resistant cultivars have not yet been developed. to produce mlbvv-resistant marker-free transgenic lettuce that would have a transgene with a promoter and terminator of lettuce origin, we constructed a two t-dna binary vector, in which the first t-dna contained the selectable marker gene neomycin phosphotransferase ii, and the second t-dna contained the let ...	2016	27055463
agrobacterium tumefaciens zur regulates the high-affinity zinc uptake system trocba and the putative metal chaperone ycic, along with zint and znuabc, for survival under zinc-limiting conditions.	agrobacterium tumefaciens has a cluster of genes (atu3178, atu3179, and atu3180) encoding an abc-type transporter, here named troa, trob, and troc, respectively, which is shown here to be a zinc-specific uptake system. reverse transcription (rt)-pcr analysis confirmed that troa, trob, and troc are cotranscribed, with troc as the first gene of the operon. the ycic (atu3181) gene is transcribed in the opposite orientation to that of the trocba operon and belongs to a metal-binding gtpase family. e ...	2016	27060116
coordinated regulation of species-specific hydroxycinnamic acid degradation and siderophore biosynthesis pathways in agrobacterium fabrum.	the rhizosphere-inhabiting species agrobacterium fabrum (genomospecies g8 of the agrobacterium tumefaciens species complex) is known to degrade hydroxycinnamic acids (hcas), especially ferulic acid and p-coumaric acid, via the novel a. fabrum hca degradation pathway. gene expression profiles of a. fabrum strain c58 were investigated in the presence of hcas, using a c58 whole-genome oligoarray. both ferulic acid and p-coumaric acid caused variations in the expression of more than 10% of the c58 g ...	2016	27060117
artificial agrobacterium tumefaciens strains exhibit diverse mechanisms to repress xanthomonas oryzae pv. oryzae-induced hypersensitive response and non-host resistance in nicotiana benthamiana.	xanthomonas oryzae pv. oryzae (xoo) rapidly triggers a hypersensitive response (hr) and non-host resistance in its non-host plant nicotiana benthamiana. here, we report that agrobacterium tumefaciens strain gv3101 blocks xoo-induced hr in n. benthamiana when pre-infiltrated or co-infiltrated, but not when post-infiltrated at 4 h after xoo inoculation. this suppression by a. tumefaciens is local and highly efficient to xoo. the hr-inhibiting efficiency of a. tumefaciens is strain dependent. strai ...	2017	27061769
molecularly characterized solvent extracts and saponins from polygonum hydropiper l. show high anti-angiogenic, anti-tumor, brine shrimp, and fibroblast nih/3t3 cell line cytotoxicity.	polygonum hydropiper is used as anti-cancer and anti-rheumatic agent in folk medicine. this study was designed to investigate the anti-angiogenic, anti-tumor, and cytotoxic potentials of different solvent extracts and isolated saponins. samples were analyzed using gc, gas chromatography-mass spectrometry (gc-ms) to identify major and bioactive compounds. quantitation of antiangiogenesis for the plant's samples including methanolic extract (ph.cr), its subsequent fractions; n-hexane (ph.hex), chl ...	2016	27065865
the n-terminal fragment of the tomato torrado virus rna1-encoded polyprotein induces a hypersensitive response (hr)-like reaction in nicotiana benthamiana.	the hypersensitive response (hr) is a defence reaction observed during incompatible plant-pathogen interactions in plants infected with a wide range of fungi, bacteria and viruses. here, we show that an n-terminal polyprotein fragment encoded by tomato torrado virus rna1, located between the first atg codon and the protease cofactor (proco) motif, induces an hr-like reaction in nicotiana benthamiana. agrobacterium tumefaciens-mediated transient expression of the first 105 amino acids (the calcul ...	2016	27072852
transient expression of viral proteins in plants using agrobacterium tumefaciens.	transient expression of viral proteins in plants is a novel alternative to other expression platforms. the viral proteins can be used as potential vaccines or in diagnostics. nicotiana benthamiana leaves or whole plants are infiltrated with recombinant agrobacterium that harbor the gene of interest. protein expression in the plants is rapid and results are obtained within 2-7 days. here we describe how to make electrocompetent agrobacterium, how to transform agrobacterium, how to infiltrate leav ...	2016	27076324
a plant-based transient expression system for the rapid production of malaria vaccine candidates.	there are currently no vaccines that provide sterile immunity against malaria. various proteins from different stages of the plasmodium falciparum life cycle have been evaluated as vaccine candidates, but none of them have fulfilled expectations. therefore, combinations of key antigens from different stages of the parasites life cycle may be essential for the development of efficacious malaria vaccines. following the identification of promising antigens using bioinformatics, proteomics, and/or i ...	2016	27076325
development of cotton leaf curl virus resistant transgenic cotton using antisense ßc1 gene.	cotton leaf curl virus (clcuv) is a serious pathogen causing leaf curl disease and affecting the cotton production in major growing areas. the transgenic cotton (gossypium hirsutum cv. coker 310) plants were developed by using βc1 gene in antisense orientation gene driven by cauliflower mosaic virus-35s promoter and nos (nopaline synthase) terminator and mediated by agrobacterium tumefaciens transformation and somatic embryogenesis system. molecular confirmation of the transformants was carried ...	2016	27081361
gas chromatography coupled with mass spectrometric characterization of curcuma longa: protection against pathogenic microbes and lipid peroxidation in rat's tissue homogenate.	the present study was designed to investigate the mineral content and antimicrobial activity of curcuma longa extracts and its essential oil. we also determined the lipid peroxidation inhibition activity of the ethanolic extract against sodium nitroprusside (snp) induced thiobarbituric acid reactive species (tbars) formation in rat's brain, kidney and liver homogenates. major constituents of essential oil identified by gas chromatography and mass spectrometry (gcms) were beta-sesquiphellandrene ...	2016	27087084
variation in growth, colonization of maize, and metabolic parameters of gfp- and dsred-labeled fusarium verticillioides strains.	autofluorescent proteins are frequently applied as visual markers in the labeling of filamentous fungi. genes gfp and dsred were transformed into the genome of fusarium verticillioides via the agrobacterium tumefaciens-mediated transformation method. the selected transformants displayed a bright green or red fluorescence in all the organelles of the growing fungal mycelia and spores (except for the vacuoles) both in cultures and in the maize (zea mays) roots they colonized. the results of gene-s ...	2016	27088391
uvhog1 is important for hyphal growth and stress responses in the rice false smut fungus ustilaginoidea virens.	rice false smut caused by ustilaginoidea virens is one of the most important diseases of rice worldwide. although its genome has been sequenced, to date there is no report on targeted gene deletion in u. virens and no molecular studies on genetic mechanisms regulating the infection processes of this destructive pathogen. in this study, we attempted to generate knockout mutants of the ortholog of yeast hog1 map kinase gene in u. virens. one uvhog1 deletion mutant was identified after screening ov ...	2016	27095476
crispr/cas9-mediated efficient and heritable targeted mutagenesis in tomato plants in the first and later generations.	the crispr/cas9 system has successfully been used in various organisms for precise targeted gene editing. although it has been demonstrated that crispr/cas9 system can induce mutation in tomato plants, the stability of heredity in later generations and mutant specificity induced by the crispr/cas9 system in tomato plants have not yet been elucidated in detail. in this study, two genes, slpds and slpif4, were used for testing targeted mutagenesis in tomato plants through an agrobacterium tumefaci ...	2016	27097775
genome sequence of the nicotine-degrading agrobacterium tumefaciens s33.	agrobacterium tumefaciens s33 is capable of growing with nicotine as the sole source of carbon and nitrogen, and has the potential to dispose of tobacco wastes and transform nicotine into functionalized pyridines intermediates, which are important precursors for some valuable drugs and insecticides. here we report the complete genome sequence of strain s33 and predict the gene cluster involved in nicotine catabolism according to the annotation.	2016	27106695
rhizobium radiobacter infection in a 27-year-old african american woman with munchausen syndrome.	rhizobium radiobacter is an opportunistic, usually saprophytic, gram-negative bacillus found in agricultural soil. isolation from blood has been reported most often in hospitalized patients harboring malignant neoplasms or human immunodeficiency virus (hiv) associated immunosuppression, who have catheter or medical device-related febrile neutropenia; treatment involves removal of the catheter or implanted medical device.(1)herein, we report a case of a 27-year-old african american woman with sic ...	2016	27107290
biotechnological approaches for biomass and cardenolide production in digitalis purpurea l.	digitalis purpurea l. is one of the main economically viable sources of cardenolides (cardiac glycosides) for the pharmaceutical industry. nevertheless, production of cardenolides in plants grown by traditional agriculture is not always an efficient process and can be affected by biotic and abiotic factors. this chapter provides two biotechnology strategies for biomass and cardenolide production in d. purpurea. firstly, we report biomass production using a temporary immersion system (tis), combi ...	2016	27108311
plant gaba:proline ratio modulates dissemination of the virulence ti plasmid within the agrobacterium tumefaciens hosted population.	accumulation of amino acids is a common plant response to several biotic and abiotic stresses, even if the roles of these accumulations remain often poorly understood. in a recent study we measured the levels of different amino acids in tumors of arabidopsis thaliana induced by the phytopathogen agrobacterium tumefaciens and correlated these data with changes of gene expressions in both organisms. this led to the demonstration that the non-protein amino acid gaba plays an important role for the ...	2016	27110651
peptidomimetic small molecules disrupt type iv secretion system activity in diverse bacterial pathogens.	bacteria utilize complex type iv secretion systems (t4sss) to translocate diverse effector proteins or dna into target cells. despite the importance of t4sss in bacterial pathogenesis, the mechanism by which these translocation machineries deliver cargo across the bacterial envelope remains poorly understood, and very few studies have investigated the use of synthetic molecules to disrupt t4ss-mediated transport. here, we describe two synthetic small molecules (c10 and ksk85) that disrupt t4ss-d ...	2016	27118587
the antibacterial activity of chitosan products blended with monoterpenes and their biofilms against plant pathogenic bacteria.	this study focuses on the biological activities of eleven chitosan products with a viscosity-average molecular weight ranging from 22 to 846 kda in combination with the most active monoterpenes (geraniol and thymol), out of 10 tested, against four plant pathogenic bacteria, agrobacterium tumefaciens, erwinia carotovora, corynebacterium fascians, and pseudomonas solanacearum. the antibacterial activity was evaluated in vitro by the agar dilution technique as a minimum inhibitory concentration (mi ...	2016	27127676
loss of podj in agrobacterium tumefaciens leads to ectopic polar growth, branching, and reduced cell division.	agrobacterium tumefaciens is a rod-shaped gram-negative bacterium that elongates by unipolar addition of new cell envelope material. approaching cell division, the growth pole transitions to a nongrowing old pole, and the division site creates new growth poles in sibling cells. the a. tumefaciens homolog of the caulobacter crescentus polar organizing protein popz localizes specifically to growth poles. in contrast, the a. tumefaciens homolog of the c. crescentus polar organelle development prote ...	2016	27137498
agrobacterium-mediated transformation of the β-subunit gene in 7s globulin protein in soybean using rnai technology.	the objective of this study was to use rna interference (rnai) to improve protein quality and decrease anti-nutritional effects in soybean. agrobacterium tumefaciens-mediated transformation was conducted using rnai and an expression vector containing the 7s globulin β-subunit gene. the bar gene was used as the selective marker and cotyledonary nodes of soybean genotype jinong 27 were chosen as explant material. regenerated plants were detected by molecular biology techniques. transformation of t ...	2016	27173254
expression of ipt in asakura-sanshoo (zanthoxylum piperitum (l.) dc. f. inerme makino) alters tree architecture, delays leaf senescence, and changes leaf essential oil composition.	the ipt gene encodes isopentenyl pyrophosphate transferase, a key enzyme in cytokinin biosynthesis. we introduced ipt under the control of the camv35s promoter into asakura-sanshoo (zanthoxylum piperitum (l.) dc. f. inerme makino) via stable agrobacterium tumefaciens-mediated transformation. three of 3-year-old transgenic asakura-sanshoo lines y5, y16, and y17 were used to evaluate the effects of ipt expression on the morphological characteristics, leaf senescence, and essential oil composition. ...	2017	27182107
engineering of corynebacterium glutamicum for xylitol production from lignocellulosic pentose sugars.	xylitol is a non-fermentable sugar alcohol used as sweetener. corynebacterium glutamicum atcc13032 was metabolically engineered for xylitol production from the lignocellulosic pentose sugars xylose and arabinose. direct conversion of xylose to xylitol was achieved through the heterologous expression of nad(p)h-dependent xylose reductase (xr) gene from rhodotorula mucilaginosa. xylitol synthesis from arabinose was attained through polycistronic expression of l-arabinose isomerase (araa), d-psicos ...	2016	27184428
agrobacterium-mediated genetic transformation of commercially elite rice restorer line using nptii gene as a plant selection marker.	transformation of commercially important indica cultivars remains challenging for the scientific community even though agrobacterium-mediated transformation protocols for a few indica rice lines have been well established. we report successful transformation of a commercially important restorer line jk1044r of indica rice hybrid jkrh 401. while following existing protocol, we optimized several parameters for callusing, regeneration and genetic transformation of jk1044r. calli generated from the ...	2016	27186018