Publications

TitleAbstractYear
Filter
PMID(sorted ascending)
Filter
novel bacterial artificial chromosome vector puvbbac for use in studies of the functional genomics of listeria spp.bacterial artificial chromosome (bac) vectors are important tools for microbial genome research. we constructed a novel bac vector, puvbbac, for replication in both gram-negative and gram-positive bacterial hosts. the puvbbac vector was used to generate a bac library for the facultative intracellular pathogen listeria monocytogenes egd-e. the library had insert sizes ranging from 68 to 178 kb. we identified two recombinant bacs from the l. monocytogenes puvbbac library that each contained the en ...200818223114
temporal transcriptomic analysis of the listeria monocytogenes egd-e sigmab regulon.the opportunistic food-borne gram-positive pathogen listeria monocytogenes can exist as a free-living microorganism in the environment and grow in the cytoplasm of vertebrate and invertebrate cells following infection. the general stress response, controlled by the alternative sigma factor, sigmab, has an important role for bacterial survival both in the environment and during infection. we used quantitative real-time pcr analysis and immuno-blot analysis to examine sigmab expression during grow ...200818226246
activity against listeria monocytogenes of human milk during lactation. a preliminary study.human milk samples from three healthy donors were investigated in order to evaluate the antibacterial activity during lactation against escherichia coli atcc 25922 and listeria monocytogenes. the concentration of the main human-milk antimicrobial proteins (lactoferrin (lf), lysozyme (lz) and secretory immunoglobulin a (siga)) was determined by elisa. results showed that human milk exhibited antibacterial activity against list. monocytogenes, although it was weakly active against esch. coli atcc ...200818226304
the listeria monocytogenes virulence factor inlj is specifically expressed in vivo and behaves as an adhesin.the food-borne pathogen listeria monocytogenes is adapted to a diversity of environments, such as soil, food, body fluids, and the cytosol of eukaryotic cells. the transition between saprophytic and pathogenic life is mediated through complex regulatory pathways that modulate the expression of virulence factors. here we examined the expression of inlj, a recently identified gene encoding a protein of the lpxtg-internalin family and involved in pathogenesis. we show that inlj expression is contro ...200818227172
activity of tigecycline against coryneform bacteria of clinical interest and listeria monocytogenes.the activities of tigecycline and eight other agents were evaluated against 220 coryneform bacteria and 42 listeria monocytogenes isolates. all strains were inhibited by tigecycline at 0.5 mug/ml, except for 11 corynebacterium striatum strains that were inhibited at 1 mug/ml. tigecycline shows good in vitro activity against coryneform bacteria and l. monocytogenes.200818227183
differential gene expression of listeria monocytogenes during high hydrostatic pressure processing.high hydrostatic pressure processing (hpp) is currently being used as a treatment for certain foods to control the presence of food-borne pathogens, such as listeria monocytogenes. genomic microarray analysis was performed to determine the effects of hpp on l. monocytogenes in order to understand how it responds to mechanical stress injury. reverse transcriptase pcr analysis of tufa and rpoc indicated that the reduction of mrna expression in hpp-treated cells was dependent on intensity and time ...200818227250
inla and inlc2 of listeria monocytogenes serotype 4b are two internalin proteins eliciting humoral immune responses common to listerial infection of various host species.the internalins inla and inlc2 are encoded proteins from two strongly immunoreactive clones recently identified by differential immunoscreening of a listeria monocytogenes serotype 4b genomic expression library during the search of the gene products of l. monocytogenes specifically induced in vivo during infection (yu wl, dan h, lin m. j med microbiol 56:888-895, 2007). in this study, we examined the humoral immune response against inla and inlc2 in various l. monocytogenes-infected hosts using ...200818228097
effects of co2 on the resuscitation of listeria monocytogenes injured by various bactericidal treatments.to assure the microbiological safety and quality of a food product, a combination of preservation hurdles is often used. therefore, the effects of carbon dioxide at concentrations of 0, 20, 40 and 60% in modified atmospheres on the resuscitation of listeria monocytogenes cells injured by mild bactericidal treatments during storage at 7 degrees c were examined. the bactericidal treatments were intense light pulses (ilp), chlorine dioxide (clo(2)), lactic acid (la) and heat. the results indicated ...200818230409
moxifloxacin versus ampicillin + gentamicin in the therapy of experimental listeria monocytogenes meningitis.this study aimed to compare the antibacterial activity of moxifloxacin and ampicillin + gentamicin in the treatment of listeria monocytogenes meningitis in a rabbit meningitis model.200818230687
effect of chemical sanitizers with and without ultrasonication on listeria monocytogenes as a biofilm within polyvinyl chloride drain pipes.as part of a biofilm in a floor drain, listeria monocytogenes is exceedingly difficult to eradicate with standard sanitizing protocols. the objective of these studies was to test the use of ultrasonication to break up biofilm architecture and allow chemical sanitizers to contact cells directly. l. monocytogenes biofilms were created in model polyvinyl chloride drain pipes. chemical sanitizers (quaternary ammonium, peroxide, or chlorine) were applied to the drain pipes with and without a 30-s ult ...200818236664
high-pressure processing of turkish white cheese for microbial inactivation.high-pressure processing (hpp) of turkish white cheese and reduction of listeria monocytogenes, total enterobacteriaceae, total aerobic mesophilic bacteria, total molds and yeasts, total lactococcus spp., and total lactobacillus spp. were investigated. cheese samples were produced from raw milk and pasteurized milk and were inoculated with l. monocytogenes after brining. both inoculated (ca. 10(7) to 10(8) cfu/g) and noninoculated samples were subjected to hpp in a high-pressure food processor a ...200818236669
inactivation of bacterial pathogens in human milk by high-pressure processing.low-temperature, long-time (ltlt) pasteurization assures the safety of banked human milk; however, heat can destroy important nutritional biomolecules. high-pressure processing (hpp) shows promise as an alternative for pasteurization of breast milk. the purpose of this study was to investigate the efficacy of hpp for inactivation of selected bacterial pathogens in human milk. human milk was inoculated with one of five pathogens (10(8) to 10(9) cfu/ml), while 0.1% peptone solution solutions with ...200818236670
antimicrobial properties of milkfat globule membrane fractions.milkfat globule membranes (mfgms) were prepared from bovine cream according to standard procedures. these membranes and peptide hydrolysates, which were generated by proteolysis with immobilized digestive enzymes, were screened for antibacterial activity against escherichia coli o157:h7, listeria monocytogenes, salmonella enterica typhimurium, pseudomonas fluorescens, bacillus cereus, lactobacillus acidophilus, and lactobacillus gasseri. assays were first performed on beef heart infusion (bhi) p ...200818236672
effect of surface roughness and stainless steel finish on listeria monocytogenes attachment and biofilm formation.the purpose of this study was to evaluate the effect of surface roughness (ra) and finish of mechanically polished stainless steel (ra = 0.26 +/- 0.05, 0.49 +/- 0.10, and 0.69 +/- 0.05 microm) and electropolished stainless steel (ra = 0.16 +/- 0.06, 0.40 +/- 0.003, and 0.67 +/- 0.02 microm) on listeria adhesion and biofilm formation. a four-strain cocktail of listeria monocytogenes was used. each strain (0.1%) was added to 200 ml of tryptic soy broth (tsb), and coupons were inserted to the mixtu ...200818236679
transfer of surface-dried listeria monocytogenes from stainless steel knife blades to roast turkey breast.listeria contamination of food contact surfaces can lead to cross-contamination of ready-to-eat foods in delicatessens. recognizing that variations in listeria biofilm-forming ability exist, the goal of this study was to determine whether these differences in biofilm formation would affect the listeria transfer rate during slicing of delicatessen turkey meat. in this study, six previously identified strong and weak biofilm-forming strains of listeria monocytogenes were grown at 22 degrees c for ...200818236680
radioresistance of salmonella species and listeria monocytogenes on minimally processed arugula (eruca sativa mill.): effect of irradiation on flavonoid content and acceptability of irradiated produce.this work studied the radiation resistance of listeria monocytogenes and salmonella species and the effect of irradiation on leaf flavonoid content and sensory acceptability of minimally processed arugula. immersion in ozone-treated water reduced the analyzed microorganisms by 1 log. l. monocytogenes and salmonella were not isolated from samples. samples of this vegetable were inoculated with a cocktail of salmonella spp. and l. monocytogenes and exposed to gamma irradiation. d10 values for salm ...200818237127
efficacy of neutral electrolyzed water (new) for reducing microbial contamination on minimally-processed vegetables.consumption of minimally-processed, or fresh-cut, fruit and vegetables has rapidly increased in recent years, but there have also been several reported outbreaks associated with the consumption of these products. sodium hypochlorite is currently the most widespread disinfectant used by fresh-cut industries. neutral electrolyzed water (new) is a novel disinfection system that could represent an alternative to sodium hypochlorite. the aim of the study was to determine whether new could replace sod ...200818237810
microbiological quality of fresh, minimally-processed fruit and vegetables, and sprouts from retail establishments.a survey of fresh and minimally-processed fruit and vegetables, and sprouts was conducted in several retail establishments in the lleida area (catalonia, spain) during 2005-2006 to determine whether microbial contamination, and in particular potentially pathogenic bacteria, was present under these commodities. a total of 300 samples--including 21 ready-to-eat fruits, 28 whole fresh vegetables, 15 sprout samples and 237 ready-to-eat salads containing from one to six vegetables--were purchased fro ...200818237811
adoptive cd8 t cell control of pathogens cannot be improved by combining protective epitope specificities.adoptive transfer of cd8 t cells has the potential to cure infectious or malignant diseases that are refractory to conventional chemotherapy. a practically important but still unanswered question is whether mixtures of protective cd8 t cells with different epitope specificities mediate more efficient effector cell functions than do the monospecific individual cd8 t cell populations. in this study, we have addressed this issue for models of viral and bacterial infection. cd8 t cell-mediated cytot ...200818240950
identification of drosophila mutants altering defense of and endurance to listeria monocytogenes infection.we extended the use of drosophila beyond being a model for signaling pathways required for pattern recognition immune signaling and show that the fly can be used to identify genes required for pathogenesis and host-pathogen interactions. we performed a forward genetic screen to identify drosophila mutations altering sensitivity to the intracellular pathogen listeria monocytogenes. we recovered 18 mutants with increased susceptibility to infection, none of which were previously shown to function ...200818245331
alkyl deoxy-arabino-hexopyranosides: synthesis, surface properties, and biological activities.octyl and dodecyl glycosides possessing 2-deoxy-arabino-hexopyranoside moieties belonging to the d- and l-series in their alpha- and beta-forms were synthesized by reaction of an acetyl protected glycal with octanol or dodecanol, catalyzed by triphenylphosphine hydrobromide, followed by deprotection. their surface properties were studied and discussed in terms of the adsorption and aggregation parameters, pc(20), cmc, and gamma(cmc). the antimicrobial activities were assessed using the paper dis ...200818249550
secreted enteric antimicrobial activity localises to the mucus surface layer.the intestinal mucosa is constantly exposed to a dense and highly dynamic microbial flora and challenged by a variety of enteropathogenic bacteria. antibacterial protection is provided in part by paneth cell-derived antibacterial peptides such as the alpha-defensins. the mechanism of peptide-mediated antibacterial control and its functional importance for gut homeostasis has recently been appreciated in patients with crohn's ileitis. in the present study, the spatial distribution of antimicrobia ...200818250125
antigen controls il-7r alpha expression levels on cd8 t cells during full activation or tolerance induction.the high-affinity chain of the il-7 receptor, il-7ralpha (cd127), is expressed by effector cd8 t cells that have the capacity to become memory cells. il-7ralpha expression is uniformly high on naive cd8 t cells, and the majority of these cells down-regulate expression upon antigenic challenge. at the peak of expansion, the fraction of effectors expressing high il-7ralpha varies depending on the response examined. the signals that a cd8 t cell receives during a response to ag that lead to altered ...200818250416
novel vaccination protocol with two live mucosal vectors elicits strong cell-mediated immunity in the vagina and protects against vaginal virus challenge.most hiv infections result from heterosexual transmission to women. because cellular immunity plays a key role in the control of the infection, we sought to strengthen cellular immune responses in vaginal tissue. we explored a novel prime-boost protocol that used two live mucosal agents that trigger different pathways of innate immunity and induce strong cellular immunity. adenovirus serotype 5 (ad5) has frequently been used as a boost for dna vaccines. in this study we used attenuated, recombin ...200818250460
occurrence of campylobacter, salmonella, yersinia enterocolitica and listeria monocytogenes in some retail food products in novi sad.the official reporting system in the province of vojvodina (pv) indicates that cases of human salmonellosis were partly covered by complete epidemiological investigation including laboratory analysis of the suspected food. intestinal campylobacteriosis and yersiniosis and four cases of septicemias caused by listeria monocytogenes were not fully epidemiologically investigated. actual country legislation on food safety does not include provisions for a routine control of the above mentioned pathog ...200718251233
pseudomonas aeruginosa activates caspase 1 through ipaf.the innate immune system encodes cytosolic nod-like receptors (nlrs), several of which activate caspase 1 processing and il-1beta and il-18 secretion. macrophages respond to salmonella typhimurium infection by activating caspase 1 through the nlr ipaf. this activation is mediated by cytosolic flagellin through the activity of the virulence-associated type iii secretion system (t3ss). we demonstrate here that pseudomonas aeruginosa activates caspase 1 and induces il-1beta secretion in infected ma ...200818256184
multiple-locus variable-number tandem-repeat analysis as a tool for subtyping listeria monocytogenes strains.listeria monocytogenes, like many other food-borne bacteria, has certain strains that are commonly linked to outbreaks. due to the relatively low numbers of affected individuals, outbreaks of l. monocytogenes can be difficult to detect. the current technique of molecular subtyping in pulsenet laboratories to identify genetically similar strains is pulsed-field gel electrophoresis (pfge). while pfge is state-of-the-art, interlaboratory comparisons are difficult because the results are highly susc ...200818256218
prophages in listeria monocytogenes contain single-nucleotide polymorphisms that differentiate outbreak clones within epidemic clones.a fragment end ligation-mediated pcr strategy was used to analyze the asci pulsed-field gel electrophoresis patterns of listeria monocytogenes epidemic clone ii (ecii), which led to the identification of single-nucleotide polymorphisms (snps) in prophage regions that differentiated the two ecii outbreak clones. snps in prophages that differentiated the outbreak clones of eciii and -iv were also identified.200818256228
platelet-derived cd154 enables t-cell priming and protection against listeria monocytogenes challenge.collagen exposure in tissue activates platelets, initiates wound healing, and modulates adaptive immunity. in this report, data are presented to demonstrate a requirement for platelet-derived cd154 for both collagen-induced augmentation of t-cell immunity and induction of pro-tective immunity to listeria challenge. specifically, we demonstrate that ad5 encoding the membrane-bound form of ovalbumin (ad5-mova) delivered in collagen induces higher ovalbumin-specific cytotoxic t lymphocyte (ctl) act ...200818256321
a role for membrane-bound cd147 in nod2-mediated recognition of bacterial cytoinvasion.nod2 is an intracellular receptor for the bacterial cell wall component muramyl dipeptide. mutations in the leucine-rich repeat region of nod2, which lead to an impaired recognition of muramyl dipeptide, have been associated with chronic inflammatory diseases of barrier organs such as crohn disease, asthma and atopic eczema. in this study we identify cd147 (also known as bsg and emmprin), a membrane-bound regulator of cellular migration, differentiation and inflammatory processes, as a protein i ...200818256385
marked intra-strain variation in response of listeria monocytogenes dairy isolates to acid or salt stress and the effect of acid or salt adaptation on adherence to abiotic surfaces.during food processing, and particularly in cheese manufacturing processes, listeria monocytogenes may be exposed routinely to environments of low ph or high salt concentration. it has been suggested that these environmental conditions may contribute to bacterial adherence to abiotic surfaces and increased resistance to disinfection. in this study strains isolated from the environment of artisanal cheese-making dairies were used to investigate the behaviour of l. monocytogenes in response to aci ...200818258322
the cytosolic sensors nod1 and nod2 are critical for bacterial recognition and host defense after exposure to toll-like receptor ligands.the cytosolic sensors nod1 and nod2 and toll-like receptors (tlrs) activate defense signaling pathways in response to microbial stimuli. however, the role of nod1 and nod2 and their interplay with tlrs during systemic bacterial infection remains poorly understood. here, we report that macrophages or mice made insensitive to tlrs by previous exposure to microbial ligands remained responsive to nod1 and nod2 stimulation. furthermore, nod1- and nod2-mediated signaling and gene expression are enhanc ...200818261938
anti-listerial activity of a polymeric film coated with hybrid coatings doped with enterocin 416k1 for use as bioactive food packaging.in this study, enterocin 416k1, a bacteriocin produced by enterococcus casseliflavus im 416k1, was entrapped in an organic-inorganic hybrid coating applied to a ldpe (low-density polyethylene) film for its potential use in the active food packaging field. the antibacterial activity of the coated film was evaluated against listeria monocytogenes nctc 10888 by qualitative modified agar diffusion assay, quantitative determination in listeria saline solution suspension and direct contact with artifi ...200818262299
x-ray and neutron small-angle scattering analysis of the complex formed by the met receptor and the listeria monocytogenes invasion protein inlb.the listeria monocytogenes surface protein inlb binds to the extracellular domain of the human receptor tyrosine kinase met, the product of the c-met proto-oncogene. inlb binding activates the met receptor, leading to uptake of listeria into normally nonphagocytic host cells. the n-terminal half of inlb (inlb(321)) is sufficient for met binding and activation. the complex between this met-binding domain of inlb and various constructs of the met ectodomain was characterized by size exclusion chro ...200818262542
response of listeria monocytogenes to liquid smoke.to investigate the effect of liquid smoke on growth, survival, proteomic pattern and haemolytic potential of listeria monocytogenes.200818266702
stimulation of inducible nitric oxide synthase expression by beta interferon increases necrotic death of macrophages upon listeria monocytogenes infection.murine macrophage death upon infection with listeria monocytogenes was previously shown to be increased by beta interferon, produced by the infected cells. we saw that interferon-upregulated caspase activation or other interferon-inducible, death-associated proteins, including trail, protein kinase r, and p53, were not necessary for cell death. macrophage death was reduced when inducible nitric oxide synthase (inos) was inhibited during infection, and inos-deficient macrophages were less suscept ...200818268032
[molecular characteristics of inlb protein of listeria monocytogenes wild strain and it's expression and purification].internalin b of listeria monocytogenes ta wild strain was amplified by pcr method and sequenced, then subcloned into prokaryotic vector pet28a for expression. the complete length of inlb gene was 1893 bp, encoding 630 amino acids. the front 35 amino acids consisted of signal peptide. there were one cap domain with alpha-helix region, 6 leucine-rich repeat motifs, one immunoglobulin (ig)-like domain, one b repeat sequence, 3 direct repeat gw domains and 5 n-glycosylation sites. the amino acid of ...200718271273
new bioactive biomaterials based on quaternized chitosan.chitosan was chemically modified to produce quaternary ammonium salts in order to improve its antimicrobial activity and physicochemical properties. quaternization of n-alkyl chitosan derivatives was carried out using alkyl iodide to elaborate water-soluble cationic polyelectrolytes ( n, n, n-trimethylchitosan, tmc). tmc was characterized by (1)h nmr spectroscopy; the quaternization degree was determined from (1)h nmr spectra and by titration of iodide ion. the antibacterial activity of hydroxyp ...200818271546
development of a listeria monocytogenes based vaccine against prostate cancer.prostate specific antigen (psa) is a likely immunotherapeutic target antigen for prostate cancer, the second leading cause of cancer-related death in american men. previously, we demonstrated that attenuated strains of listeria monocytogenes (lm) can be used as effective vaccine vectors for delivery of tumor antigens causing regression of established tumors accompanied by strong immune responses toward these antigens in murine models of cancer. in the present study, we have developed and charact ...200818273616
listeria monocytogenes meningoencephalitis presenting with hydrocephalus and ventriculitis. 200818277040
influence of processing steps in cold-smoked salmon production on survival and growth of persistent and presumed non-persistent listeria monocytogenes.cold-smoked salmon is a ready-to-eat product in which listeria monocytogenes sometimes can grow to high numbers. the bacterium can colonize the processing environment and it is believed to survive or even grow during the processing steps. the purpose of the present study was to determine if the steps in the processing of cold-smoked salmon affect survival and subsequent growth of a persistent strain of l. monocytogenes to a lesser degree than presumed non-persistent strains. we used a sequence o ...200818279988
activity of daptomycin against listeria monocytogenes isolates from cerebrospinal fluid.we tested the activity of daptomycin against 76 listeria monocytogenes isolates from cerebrospinal fluid by broth dilution and etest methods. for the broth dilution method, the mic range was 1.0 to 8.0 and the mic at which 90% of the isolates tested were inhibited (mic(90)) was 4.0 mg/liter. for the etest method, the mic range was 1.0 to 4.0 and the mic(90) was 4.0 mg/liter. presently, daptomycin cannot be recommended for the treatment of l. monocytogenes meningitis.200818285470
cutting edge: il-27 is a potent inducer of il-10 but not foxp3 in murine t cells.the cytokine il-27 is important for restricting inflammation in response to a wide variety of immune challenges. in this study, we demonstrate that il-27 induces expression of the anti-inflammatory cytokine il-10 by cd4+ and cd8+ t cells. il-27 relied upon the th1 transcription factor stat1 to induce il-10+ifn-gamma+foxp3- th1 cells, which were recently shown to be key negative regulators during certain infections. il27ra-/- mice generated fewer il-10+ t cells during both listeria monocytogenes ...200818292493
constitutive expression of il-7 receptor alpha does not support increased expansion or prevent contraction of antigen-specific cd4 or cd8 t cells following listeria monocytogenes infection.expression of il-7ralpha (cd127) has been suggested as a major determinant in the survival of memory t cell precursors. we investigated whether constitutive expression of il-7ralpha on t cells increased expansion and/or decreased contraction of endogenous ag-specific cd4 and cd8 t cells following infection with listeria monocytogenes. the results indicate that constitutive expression of il-7ralpha alone was not enough to impart an expansion or survival advantage to cd8 t cells responding to infe ...200818292507
mutation in the fas pathway impairs cd8+ t cell memory.fas death pathway is important for lymphocyte homeostasis, but the role of fas pathway in t cell memory development is not clear. we show that whereas the expansion and contraction of cd8+ t cell response against listeria monocytogenes were similar for wild-type (wt) and fas ligand (fasl) mutant mice, the majority of memory cd8+ t cells in fasl mutant mice displayed an effector memory phenotype in the long-term in comparison with the mainly central memory phenotype displayed by memory cd8+ t cel ...200818292515
stimulation of enhanced cd8 t cell responses following immunization with a hyper-antigen secreting intracytosolic bacterial pathogen.the intracytosolic niche for replication of listeria monocytogenes (lm) facilitates delivery of bacteria-derived ags into the mhc class i pathway for subsequent stimulation of cd8 effector t cells. using lm strains that are equivalent for in vivo virulence yet express marked differences in the level of secretion of a protective target ag, we have evaluated how these specific differences in secretion levels influences the magnitude and effector function of ag-specific cd8 t cell responses followi ...200818292567
[synthesis and antimicrobial activity of some new (sulfon-amidophenyl)-amide derivatives of n-(m-nitrobenzoyl)-d,l-methionine].in order to obtain new compounds with potential antimicrobial activity some new (sulfon-amidophenyl)-amide derivatives of n-(m-nitrobenzoil)-d,l-methionine have been synthesized. the compounds have been obtained by decyclization reaction of 2-(m-nitrophenyl)-4-(beta-p-methylthioethyl)- delta2-5-oxazolinone with various sulfonamides. the chemical structure of the new sulfonamides was confirmed by elemental analysis data and ir and 1nmr spectral measurements. the compounds were also investigated f ...200718293716
periaortic endograft infection due to listeria monocytogenes treated with graft preservation.a 67-year-old man presented to our hospital with general malaise, fever and diffuse abdominal and lower back pain 7 weeks after endovascular aneurysm repair. blood samples showed a leukocyte count of 10.9 x 10(9)/l and a c-reactive protein of 239 mg/l. the computed tomography (ct)-scan showed fluid collections behind the proximal part of the endovascular graft and dorsal to the aorta. ct-guided translumbar needle aspiration of these collections yielded growth with listeria monocytogenes. prosthe ...200818295117
a "retrocidal" plasmid in enterococcus faecalis: passage and protection.enterococcus faecalis mc4 harbors a 130 kb conjugative, pheromone (ccf10)-responding plasmid, pams1, conferring chloramphenicol, streptomycin and tetracycline resistances. a plasmid-borne class iia bacteriocin (mc4-1) determinant and cognate immunity gene were present, but not expressed in mc4. however, pams1 transfer to e. faecalis jh2-2 (but not to the non-isogenic og1ss) generated the surprising ability to express bacteriocin activity against the plasmid donor, mc4. the bacteriocin target spe ...200818295881
restricted expression of c-type lectin-like natural killer receptors by cd8 t cells in the murine small intestine.the intestinal mucosa represents a challenging environment for cd8+ t cells, which must tolerate nutrient antigens and commensal microorganisms while responding efficiently to pathogens. consequently, specific regulatory mechanisms apply for cd8+ t cells in the intestinal environment, which should also be reflected in a tissue-specific gene expression profile of these cells. this study investigates whether such tissue-specific gene expression can be observed in cd8+ t cells primed during bacteri ...200818298548
detection of sublethal thermal injury in salmonella enterica serotype typhimurium and listeria monocytogenes using fourier transform infrared (ft-ir) spectroscopy (4000 to 600 cm(-1)).fourier transform infrared (ft-ir) spectroscopy (4000 to 600 cm(-1)) was utilized to detect sublethally heat-injured microorganisms: salmonella enterica serotype typhimurium atcc 14028, a gram-negative bacterium, and listeria monocytogenes atcc 19113, a gram-positive bacterium. a range of heat treatments (n= 2) at 60 degrees c were evaluated: 0d (control), 2d, 4d, 6d, and 8d using a d(60 degrees c) (s. enterica serotype typhimurium atcc 14028 = 0.30 min, l. monocytogenes atcc 19113 = 0.43 min). ...200818298736
control of listeria monocytogenes and salmonella anatum on the surface of smoked salmon coated with calcium alginate coating containing oyster lysozyme and nisin.this study investigated the antimicrobial effect of oyster lysozyme with or without nisin added to calcium alginate (caalg) coated on the surface of smoked salmon against listeria monocytogenes and salmonella anatum. l. monocytogenes or s. anatum inoculated smoked salmon samples (1 g) were dipped into caalg with either oyster lysozyme (oysl) or hen egg white lysozyme (hewl), with or without added nisin (n), then stored at 4 degrees c for 35 d. our results indicated that the effectiveness of oyst ...200818298738
inactivation of listeria innocua on frankfurters that contain potassium lactate and sodium diacetate by flash pasteurization.listeria monocytogenes, a psychrotrophic foodborne pathogen, is a recurring postprocess contaminant on ready-to-eat meat (rte) products, including frankfurters. potassium lactate (pl) and sodium diacetate (sda) are fda-approved antimicrobials that inhibit the growth of l. monocytogenes when incorporated into the formulation of fine emulsion sausage. flash (steam) pasteurization (fp) has been shown to reduce levels of l. monocytogenes, and its surrogate l. innocua, on frankfurter surfaces. the ab ...200818298739
comparison of the in vitro efficacies of moxifloxacin and amoxicillin against listeria monocytogenes.listeria monocytogenes is a facultative intracellular bacterium that causes severe infections associated with a high mortality rate. moxifloxacin presents extended activity against gram-positive bacteria and has recently been suggested to be a potential alternative in the treatment of listeriosis. we evaluated the in vitro efficacy of moxifloxacin against l. monocytogenes using a combination of epidemiological and experimental approaches. the median mic of moxifloxacin for a large collection of ...200818299415
quantitative evaluation of interleukin-12 p40 gene expression in peripheral blood mononuclear cells.the heterodimeric cytokine il-12 (composed of a p35 and a p40 subunit) is produced primarily by monocytes, macrophages and b cells. in vitro and in vivo experiments have demonstrated the crucial role of il-12 in initiating and establishing both innate immunity and t cell-mediated resistance to intracellular pathogens, including leishmania donovani, toxoplasma gondii, listeria monocytogenes, and mycobacterium tuberculosis. assessment of cytokine expression has thus become crucial to understand ho ...200818300039
monocyte-mediated defense against microbial pathogens.circulating blood monocytes supply peripheral tissues with macrophage and dendritic cell (dc) precursors and, in the setting of infection, also contribute directly to immune defense against microbial pathogens. in humans and mice, monocytes are divided into two major subsets that either specifically traffic into inflamed tissues or, in the absence of overt inflammation, constitutively maintain tissue macrophage/dc populations. inflammatory monocytes respond rapidly to microbial stimuli by secret ...200818303997
potential antimicrobials to control listeria monocytogenes in vacuum-packaged cold-smoked salmon pâté and fillets.in the wake of recent outbreaks associated with listeria monocytogenes in ready-to-eat foods and an increasing desire for minimally processed foods, there has been a burgeoning interest in the use of natural antimicrobials by the food industry to control this pathogen. the minimum inhibitory concentrations (mics) of nisin and salts of organic acids (sodium lactate (sl), sodium diacetate (sd), sodium benzoate (sb), and potassium sorbate (ps)) against twelve strains of l. monocytogenes in a tsbye ...200818308410
multiple point mutations in virulence genes explain the low virulence of listeria monocytogenes field strains.in order to understand the causes of the low virulence of listeria monocytogenes field strains, five low-virulence strains were analysed. these five strains showed changes in relation to invasion, phosphatidyl-inositol phospholipase c (pi-plc) activity, plaque formation and in vivo virulence. molecular analyses revealed the same mutations in the plca, inla and inlb genes in all five strains. the thr262ala substitution in the pi-plc protein was responsible for the absence of pi-plc activity. this ...200818310040
interactions between food-borne pathogens and protozoa isolated from lettuce and spinach.the survival of salmonella enterica was recently shown to increase when the bacteria were sequestered in expelled food vacuoles (vesicles) of tetrahymena. because fresh produce is increasingly linked to outbreaks of enteric illness, the present investigation aimed to determine the prevalence of protozoa on spinach and lettuce and to examine their interactions with s. enterica, escherichia coli o157:h7, and listeria monocytogenes. glaucoma sp., colpoda steinii, and acanthamoeba palestinensis were ...200818310421
optical tweezers cause physiological damage to escherichia coli and listeria bacteria.we investigated the degree of physiological damage to bacterial cells caused by optical trapping using a 1,064-nm laser. the physiological condition of the cells was determined by their ability to maintain a ph gradient across the cell wall; healthy cells are able to maintain a ph gradient over the cell wall, whereas compromised cells are less efficient, thus giving rise to a diminished ph gradient. the ph gradient was measured by fluorescence ratio imaging microscopy by incorporating a ph-sensi ...200818310432
analysis of time-resolved scattering from macroscale bacterial colonies.we investigate the relationship of incubation time and forward-scattering signature for bacterial colonies grown on solid nutrient surfaces. the aim of this research is to understand the colony growth characteristics and the corresponding evolution of the scattering patterns for a variety of pathogenic bacteria relevant to food safety. in particular, we characterized time-varying macroscopic and microscopic morphological properties of the growing colonies and modeled their optical properties in ...200818315368
listeria monocytogenes activated dendritic cell based vaccine for prevention of experimental tumor in mice.the use of dendritic cells (dcs) as a cellular adjuvant provides a promising approach in immunotherapy of cancer. it has been demonstrated that listeria monocytogenes activated dcs pulsed ex vivo with tumor antigens trigger a systemic th1-biased specific immune response and a single dose of this vaccine will cause a considerable anti tumor immunity.200818319523
application of pcr-sscp for molecular epidemiological studies on the exposure of farm children to bacteria in environmental dust.the environmental exposure of farm children to microorganisms in dust has become a focus of interest, since microbial exposure on farms has been related to a reduced prevalence of asthma and atopic diseases in children. previous studies almost exclusively focused on the determination of microbial counts using conventional culturing or the determination of microbial compounds i.e. endotoxins. in this study pcr-sscp (single-strand conformation polymorphism) was modified for characterising bacteria ...200818321601
role of interleukin-12 in determining differential kinetics of invariant natural killer t cells in response to differential burden of listeria monocytogenes.invariant (i) natural killer (nk) t cells are unique t lymphocytes expressing nkr-p1b/c (nk1.1), which recognize glycolipids, notably alpha-galactosylceramide (alpha-galcer) presented by cd1d. the characteristic phenotype of these inkt cells undergoes dramatic changes following listeria monocytogenes infection, and interleukin (il)-12 is involved in these alterations. here we show that liver inkt cells in mice are differentially influenced by the load of infection. liver alpha-galcer/cd1d tetram ...200818321748
deviation from a strong th1-dominated to a modest th17-dominated cd4 t cell response in the absence of il-12p40 and type i ifns sustains protective cd8 t cells.the differentiation of naive cd4 t cells into specific effector subsets is controlled in large part by the milieu of cytokines present during their initial encounter with ag. cytokines that drive differentiation of the newly described th17 lineage have been characterized in vitro, but the cytokines that prime commitment to this lineage in response to infection in vivo are less clear. listeria monocytogenes (lm) induces a strong th1 response in wild-type mice. by contrast, we demonstrate that in ...200818322221
ifn-beta increases listeriolysin o-induced membrane permeabilization and death of macrophages.type i ifn (ifn-i) signaling is detrimental to cells and mice infected with listeria monocytogenes. in this study, we investigate the impact of ifn-i on the activity of listeriolysin o (llo), a pore-forming toxin and virulence protein released by l. monocytogenes. treatment of macrophages with ifn-beta increased the ability of sublytic llo concentrations to cause transient permeability of the plasma membrane. at higher llo concentrations, ifn-beta enhanced the complete breakdown of membrane inte ...200818322222
antibacterial activity of tannin constituents from phaseolus vulgaris, fagoypyrum esculentum, corylus avellana and juglans nigra.tannins were fractionated from acetonic extract preparations of phenolic compounds from phaseolus vulgaris, fagoypyrum esculentum, corylus avellana and juglans nigra. wide variations in antibacterial activities ranging from mics of 62.5 to 500 microg/ml were apparent. of particular note was a relatively high level of activity (62.5 to 125 microg/ml) for all extracts against listeria monocytogenes.200818325686
microbiological safety of sandwiches from hospitals and other health care establishments in the united kingdom with a focus on listeria monocytogenes and other listeria species.in the united kingdom between 1999 and 2004, there were four outbreaks of listeria monocytogenes infection associated with sandwiches purchased from or provided in hospitals. elderly or immunocompromised individuals and pregnant women are particularly vulnerable to infection; therefore, the focus of this study was on sandwiches served in health care establishments. of 3,249 sandwich samples collected between april 2005 and march 2006, 3.3% were of unsatisfactory microbiological quality because o ...200818326180
antimicrobial activity of lactoferrin against foodborne pathogenic bacteria incorporated into edible chitosan film.the objectives of this research were to develop and characterize edible chitosan film containing lactoferrin as a natural antimicrobial agent, and to investigate the combination effects of lactoferrin with lysozyme in chitosan film against the growth of escherichia coli o157:h7 and listeria monocytogenes. chitosan films containing lactoferrin, lysozyme, or nisin were fabricated, and the antimicrobial concentrations were 0.5, 1, or 2 mg in a circular disc of chitosan film. three concentrations of ...200818326181
growth inhibitory effects of kimchi (korean traditional fermented vegetable product) against bacillus cereus, listeria monocytogenes, and staphylococcus aureus.kimchi is a unique korean traditional vegetable product that is fermented by lactic acid bacteria (lab) and is mainly consumed as a side dish with boiled rice. its main ingredients are brined chinese cabbage, red pepper powder, and fermented fish sauce, and these are combined with many spices such as garlic, green onion, ginger, and some seaweed. the relationship between the concentration of lab or the ph and the growth of three gram-positive foodborne pathogens (bacillus cereus, listeria monocy ...200818326182
foodnet survey of food use and practices in long-term care facilities.foodborne illness is an important problem among the elderly. one risk factor for foodborne illness and diarrhea-associated mortality among the elderly is residence in a long-term care facility (ltcf); thus, these facilities must implement measures to ensure safe food. to assess safe food practices, knowledge, and policies, we used a mailed, self-administered questionnaire to survey food service directors at ltcfs that were certified to receive medicare or medicaid at eight foodborne diseases act ...200818326188
contributions of two-component regulatory systems, alternative sigma factors, and negative regulators to listeria monocytogenes cold adaptation and cold growth.the ability of listeria monocytogenes to grow at refrigeration temperatures is critical for transmission of this foodborne pathogen. we evaluated the contributions of different transcriptional regulators and two-component regulatory systems to l. monocytogenes cold adaptation and cold growth. l. monocytogenes parent strain 10403s and selected isogenic null mutants in genes encoding four alternative sigma factors (sigb, sigh, sigc, and sigl), two regulators of sigmab (rsbt and rsbv), two negative ...200818326199
heat shock induces barotolerance in listeria monocytogenes.the aim of this study was to investigate the effect of heat shock on the resistance of listeria monocytogenes to high pressure processing (hpp). l. monocytogenes atcc 19115 was grown to stationary phase at 15 degrees c and inoculated into whole ultrahigh-temperature milk at approximately 10(7) cfu/ml. milk samples (5 ml) were placed into plastic transfer pipettes, which were heat sealed and then heated in a water bath at 48 degrees c for 10 min. immediately after heat shock, the milk was cooled ...200818326200
listeria monocytogenes internalins bind to the human intestinal mucin muc2.listeria monocytogenes cross the intestinal barrier causing systemic infections with high mortality rates. intestinal infection triggers release of intestinal mucus. we show that three l. monocytogenes internalins, inlb, inlc and inlj all bound to muc2 (the major component of intestinal mucus), but not to the cell surface mucin muc1. binding was strongest to inlb>inlc>inlj (p < 0.001). listerial internalins are characterized by their internalin domain, composed by leucine rich repeats (lrr) foll ...200818327567
[perparation and characterization of the monoclonal antibodies against listeriolysin o].to prepare the monoclonal antibodies (mabs) against listeriolysin o (llo), which is the major virulence factor of listeria monocytogenes.200818328183
inhibition of rock activity allows inlf-mediated invasion and increased virulence of listeria monocytogenes.listeria monocytogenes is an intracellular bacterial pathogen that causes life-threatening disease. the mechanisms used by l. monocytogenes to invade non-professional phagocytic cells are not fully understood. in addition to the requirement of bacterial determinants, host cell conditions profoundly influence infection. here, we have shown that inhibition of the rhoa/rock pathway by pharmacological inhibitors or rna interference results in increased l. monocytogenes invasion of murine fibroblasts ...200818331468
keratinocyte growth factor and androgen blockade work in concert to protect against conditioning regimen-induced thymic epithelial damage and enhance t-cell reconstitution after murine bone marrow transplantation.myeloablative conditioning results in thymic epithelial cell (tec) injury, slow t-cell reconstitution, and a high risk of opportunistic infections. keratinocyte growth factor (kgf) stimulates tec proliferation and, when given preconditioning, reduces tec injury. thymocytes and tecs express androgen receptors, and exposure to androgen inhibits thymopoiesis. in this study, we have investigated whether tec stimulation via preconditioning treatment with kgf and leuprolide acetate (lupron), 2 clinica ...200818334670
on the nature of antimicrobial activity: a model for protegrin-1 pores.we conducted over 150 ns of simulation of a protegrin-1 octamer pore in a lipid bilayer composed of palmitoyloleoyl-phosphatidylethanolamine (pope) and palmitoyloleoyl-phosphatidylglycerol (popg) lipids mimicking the inner membrane of a bacterial cell. the simulations improve on a model of a pore proposed from recent nmr experiments and provide a coherent understanding of the molecular mechanism of antimicrobial activity. although lipids tilt somewhat toward the peptides, the simulated protegrin ...200818335931
crystal structure and standardized geometric analysis of inlj, a listerial virulence factor and leucine-rich repeat protein with a novel cysteine ladder.we report on the crystal structure of the internalin domain of inlj, a virulence-associated surface protein of listeria monocytogenes, at 2.7-a resolution. inlj is a member of the internalin family of listerial cell surface proteins characterized by a common n-terminal domain. inlj bears 15 leucine-rich repeats (lrrs), the same number as in inla, the prototypical internalin family member. the lrrs of inlj differ from those of other internalins by having 21, rather than 22, residues and by replac ...200818343406
qnr-like pentapeptide repeat proteins in gram-positive bacteria.to study the role of qnr-like pentapeptide repeat proteins (prps) from several gram-positive species with quinolone resistance in vitro.200818343805
a comparison between polymeric microsphere and bacterial vectors for macrophage p388d1 gene delivery.the purpose of this study was to compare bacterial and polymeric gene delivery devices for the ability to deliver plasmid dna to a murine macrophage p388d1 cell line.200818343983
survival and growth of foodborne microorganisms in processed and individually wrapped cheese slices.the objectives of the research reported here were to determine the growth, survival, or inactivation of selected microorganisms on individually wrapped processed cheese (iwc) slices stored at 5 degrees c and 22 degrees c, and to compare quality indices. iwc slices were spot-inoculated with foodborne pathogenic bacteria (listeria monocytogenes, staphylococcus aureus, and salmonella spp.), spoilage bacteria (pseudomonas spp. and lactobacillus spp.), and spoilage molds (penicillium spp. and cladosp ...200818348389
virulence of listeria monocytogenes isolated from the cheese dairy environment, other foods and clinical cases.the virulence potential of 51 listeria monocytogenes isolates, including strains from cheese, cheese production environments and from human cases of listeriosis, was evaluated in this study. the isolates were used to infect ht-29 cell monolayers in an in vitro test of virulence, based on a plaque-forming assay (pfa). fifteen selected isolates were used for subcutaneous footpad inoculation in mice and subsequent recovery of the bacterium from the spleen 3 days after inoculation. in the pfa, two i ...200818349357
[neonatal septicemia caused by listeria monocytogenes: report of 6 cases].neonatal listeriosis is a relatively rare but serious disease with a high mortality rate. this study was conducted to analyze the clinical features, treatment, and outcome of 6 cases with listeria monocytogenes septicemia confirmed by positive blood cultures.200818353233
trail deficiency does not rescue impaired cd8+ t cell memory generated in the absence of cd4+ t cell help.ag-specific cd8(+) t cells immunized in the absence of cd4(+) t cell help, so-called "unhelped" cd8(+) t cells, are defective in function and survival. we investigated the role of the proapoptotic molecule trail in this defect. we first demonstrate that trail does not contribute to the cd8(+) t cell response to listeria monocytogenes strain expressing ova (lmova) in the presence of cd4(+) t cells. secondly, we generated mice doubly deficient in cd4(+) t cells and trail and analyzed their cd8(+) ...200818354179
the inflammasome: a key player in the inflammation triggered in response to bacterial pathogens. 200818354318
comparison of primary predictive models to study the growth of listeria monocytogenes at low temperatures in liquid cultures and selection of fastest growing ribotypes in meat and turkey product slurries.this study compared the performance of four primary mathematical models to study the growth kinetics of listeria monocytogenes ribotypes grown at low temperature so as to identify the best predictive model. the parameters of the best-fitting model were used to select the fastest growing strains with the shortest lag time and greatest growth rate. nineteen food, human and animal l. monocytogenes isolates with distinct ribotype were grown at 4, 8, and 12 degrees c in tryptic soy broth and slurries ...200818355671
potential of a nisin-containing bacterial cellulose film to inhibit listeria monocytogenes on processed meats.a bacterially produced cellulose film containing nisin was developed and used in a proof-of-concept study to control listeria monocytogenes and total aerobic bacteria on the surface of vacuum-packaged frankfurters. bacterial cellulose pellicles were produced by gluconacetobacter xylinus k3 in corn steep liquor-mannitol medium and were subsequently purified before nisin was incorporated into them. investigations into the effect of nisin concentrations and contact times on incorporation of nisin i ...200818355672
combination of high-intensity pulsed electric fields with natural antimicrobials to inactivate pathogenic microorganisms and extend the shelf-life of melon and watermelon juices.the effect of high-intensity pulsed electric field (hipef) combined with citric acid (0.5-2.0%, w/v) or cinnamon bark oil (0.05-0.30%, w/v) against populations of escherichia coli o157:h7, salmonella enteritidis and listeria monocytogenes in melon and watermelon juices were evaluated. microbiological shelf-life and sensory attributes were also determined. populations of e. coli o157:h7, s. enteritidis and l. monocytogenes were reduced by more than 5.0log(10)cfu/ml in hipef-processed melon (35kv/ ...200818355673
edible chitosan films on ready-to-eat roast beef for the control of listeria monocytogenes.the use of chitosan as an edible film was evaluated for its antimicrobial activity against listeria monocytogenes (lm) on the surface of ready-to-eat (rte) roast beef. l. monocytogenes, decimally diluted to give an initial inoculation of >6.50logcfu/g, was inoculated onto the surface of rte roast beef cubes, and air-dried. the samples were dipped into chitosan (high or low molecular weights) solutions dissolved with acetic or lactic acid at 0.5% (w/v) or 1% (w/v) then bagged and refrigerated at ...200818355679
rapid culling of the cd4+ t cell repertoire in the transition from effector to memory.requirements for cd4+ t cell memory differentiation were analyzed with adoptively transferred smarta t cell receptor (tcr) transgenic cells specific for alymphocytic choriomeningitis virus (lcmv) epitope. lcmv-induced effector and memory differentiation of smarta cells mimicked the endogenous cd4+ t cell response. in contrast, infection with a recombinant listeria expressing the lcmv epitope, although resulting initially in massive smarta expansion, led to loss of effector function and rapid cel ...200818356084
modeling transfer of listeria monocytogenes from slicer to deli meat during mechanical slicing.listeria monocytogenes has been implicated in several listeriosis outbreaks linked to the consumption of presliced ready-to-eat (rte) deli meats. the possible contamination of sliced rte meats by l. monocytogenes during the slicing process has become a public health concern. the objectives of this study were to investigate the transfer phenomena of l. monocytogenes between a meat slicer and ham slices, and to develop empirical models to describe the transfer during slicing. a six-strain cocktail ...200818361687
[relations of the causative agents of plague and listeriosis during their simultaneous stay in the flea citellophilus tesquorum at different environmental temperatures].experiments were carried out to determine the persistence of a plague microbe with varying praline labeling (isolated in the central caucasian mountain focus of plague in the fleas citellophilus tesquorum of a mountain subspecies) in the contamination of the mixed culture of the plague microbe and listeria. inoculation of fleas with a suspension of a plague microbe and listeriae demonstrates the manifest signs of antagonism between both species of microorganisms. this is evidenced by the reducti ...200818368716
evolution, expression and effectiveness in a cluster of novel bovine beta-defensins.the anti-microbial peptides beta-defensins constitute a large family of innate immune effector molecules, conserved across a wide species range. in this paper, we describe a systematic search of the sequenced bovine genome to characterise this extensive gene family in bos taurus, providing an insight into the pattern of conservation of beta-defensin genes between species. we have sequenced a sub-set of these newly discovered bovine beta-defensin genes and also report expression data for these ge ...200818369613
polyclonal and antigen-specific responses of t cells and t cell subsets.evaluation of the functional responses of t cells is of importance in determining the mechanism(s) of immunodeficiency resulting from chronic alcohol abuse and other conditions that lead to immune dysfunction. mice that are chronically exposed to 20% (w/v) ethanol in water develop immunodeficiency and have t cells with abnormal activation profiles, reduced total numbers, increased cd4/cd8 ratios, and an increased memory/naïve phenotype ratio. these cells also have abnormal antigen-specific respo ...200818369925
bioluminescence imaging to evaluate infections and host response in vivo.the continued prospect of emerging pathogens and recent events including the acceptance of widespread drug resistance and threats of bioterrorism have introduced the necessity be creative in our development of therapies for bacterial infections. many pathogens have both acute and persistent phases. there is a need to understand these pathogens throughout their entire life cycle within the host and determine the role that the host response including innate immunity plays in the establishment and ...200818370150
[identification of proteins determining the virulence of listeria monocytogenes using the bioinformation analysis].the postgenomic stage of biotechnology allows the bioinformation approaches to be used for revealing previously unknown factors involved in different processes. in this work, bioinformation approaches were applied to analysis of factors involved in the l. monocytogenes virulence. several open reading frames (orfs) were identified in l. monocytogenes genome, which encode proteins with high level homology to bacterial peptidases and which meets the developed criteria. the influence of the orfs on ...200818376476
the antimicrobial efficacy of plant essential oil combinations and interactions with food ingredients.the objective of this study was to evaluate the efficacy of plant essential oils (eos) in combination and to investigate the effect of food ingredients on their efficacy. the eos assessed in combination included basil, lemon balm, marjoram, oregano, rosemary, sage and thyme. combinations of eos were initially screened against bacillus cereus, escherichia coli, listeria monocytogenes and pseudomonas aeruginosa using the spot-on-agar test. the influence of varying concentrations of eo combinations ...200818378032
assessing genetic heterogeneity within bacterial species isolated from gastrointestinal and environmental samples: how many isolates does it take?strain typing of bacterial isolates is increasingly used to identify sources of infection or product contamination and to elucidate routes of transmission of pathogens or spoilage organisms. usually, the number of bacterial isolates belonging to the same species that is analyzed per sample is determined by convention, convenience, laboratory capacity, or financial resources. statistical considerations and knowledge of the heterogeneity of bacterial populations in various sources can be used to d ...200818378649
effect of antimicrobial peptides divergicin m35 and nisin a on listeria monocytogenes lsd530 potassium channels.the aim of this work was to study the effect of antimicrobial peptides: divergicin m35 and nisin a on listeria monocytogenes lsd 530 potassium (k+) channels: atp-sensitive (k atp), calcium-activated (bk ca), and depolarization-activated (kv) types. increase on k+ efflux and inhibition of cellular growth were observed after adding k+ channel activators pinacidil, ns1619, and cromakalim to divergicin m35. increase in k+ efflux from log-phase cells was about 18 +/- 1.1, 11 +/- 0.63, and nmol mg(-1) ...200818379845
Refresh
firstprev
...101102103104105106107108109110...
nextlast
Displaying items 10201 - 10300 of 16792