Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| sugarcane (saccharum spp. hybrids). | genetic transformation of sugarcane has a tremendous potential to complement traditional breeding in crop improvement and will likely transform sugarcane into a bio-factory for value-added products. we describe here agrobacterium tumefaciens-mediated transformation of sugarcane. embryogenic callus induced from immature leaf whorls was used as target for transformation with the hypervirulent agrobacterium strain agl1 carrying a constitutive nptii expression cassette in vector ppzp200. selection w ... | 2015 | 25416267 |
| hemp (cannabis sativa l.). | hemp (cannabis sativa l.) suspension culture cells were transformed with agrobacterium tumefaciens strain eha101 carrying the binary plasmid pnov3635. the plasmid contains a phosphomannose isomerase (pmi) selectable marker gene. cells transformed with pmi are capable of metabolizing the selective agent mannose, whereas cells not expressing the gene are incapable of using the carbon source and will stop growing. callus masses proliferating on selection medium were screened for pmi expression usin ... | 2015 | 25416268 |
| poinsettia (euphorbia pulcherrima willd. ex klotzsch). | genetic engineering is an important tool for introducing desired genes into poinsettia (euphorbia pulcherrima willd. ex klotzsch). we describe in this chapter an agrobacterium tumefaciens-mediated transformation protocol for poinsettia. a detailed description of genetic transformation, antibiotic selection, subsequent regeneration via somatic embryogenesis, and rooting as well as molecular and morphological analyses is included. the methodology described here could facilitate the future engineer ... | 2015 | 25416270 |
| tall fescue (festuca arundinacea schreb.). | tall fescue (festuca arundinacea schreb.) is the predominant cool-season perennial grass in the united states. it is widely used for both forage and turf purposes. this chapter describes a protocol that allows for the generation of a large number of transgenic tall fescue plants by agrobacterium tumefaciens-mediated transformation. embryogenic calli induced from caryopsis are used as explants for inoculation with a. tumefaciens. the agrobacterium strain used is eha105. hygromycin phosphotransfer ... | 2015 | 25416272 |
| construction of infectious clones of tomato torrado virus and their delivery by agroinfiltration. | the first biologically active infectious clones of tomato torrado virus (totv) were generated and delivered into nicotiana benthamiana and solanum lycopersicum plants via agrobacterium tumefaciens. the engineered constructs consisted of pcr-amplified complementary dnas derived from the totv rna1 and rna2 components, individually inserted into an engineered pgreen binary vector between the camv 35s promoter and nopaline synthase terminator. these constructs were introduced into the plant hosts by ... | 2015 | 25416854 |
| global and phylogenetic distribution of quorum sensing signals, acyl homoserine lactones, in the family of vibrionaceae. | bacterial quorum sensing (qs) and the corresponding signals, acyl homoserine lactones (ahls), were first described for a luminescent vibrio species. since then, detailed knowledge has been gained on the functional level of qs; however, the abundance of ahls in the family of vibrionaceae in the environment has remained unclear. three hundred and one vibrionaceae strains were collected on a global research cruise and the prevalence and profile of ahl signals in this global collection were determin ... | 2014 | 25419995 |
| efficient transformation and expression of gfp gene in valsa mali var. mali. | valsa mali var. mali, the causal agent of valsa canker of apple, causes great loss of apple production in apple producing regions. the pathogenic mechanism of the pathogen has not been studied extensively, thus a suitable gene marker for pathogenic invasion analysis and a random insertion of t-dna for mutants are desirable. in this paper, we reported the construction of a binary vector pko1-hph containing a positive selective gene hygromycin phosphotransferase (hph), a reporter gene gfp conferri ... | 2015 | 25423905 |
| blocking single-stranded transferred dna conversion to double-stranded intermediates by overexpression of yeast dna replication factor a. | agrobacterium tumefaciens delivers its single-stranded transferred dna (t-strand) into the host cell nucleus, where it can be converted into double-stranded molecules. various studies have revealed that double-stranded transfer dna (t-dna) intermediates can serve as substrates by as yet uncharacterized integration machinery. nevertheless, the possibility that t-strands are themselves substrates for integration cannot be ruled out. we attempted to block the conversion of t-strands into double-str ... | 2015 | 25424309 |
| progress of cereal transformation technology mediated by agrobacterium tumefaciens. | monocotyledonous plants were believed to be not transformable by the soil bacterium agrobacterium tumefaciens until two decades ago, although convenient protocols for infection of leaf disks and subsequent regeneration of transgenic plants had been well established in a number of dicotyledonous species by then. this belief was reinforced by the fact that monocotyledons are mostly outside the host range of crown gall disease caused by the bacterium and by the failures in trials in monocotyledons ... | 2014 | 25426132 |
| novel compounds that enhance agrobacterium-mediated plant transformation by mitigating oxidative stress. | agrobacterium tumefaciens caused tissue browning leading to subsequent cell death in plant transformation and novel anti-oxidative compounds enhanced agrobacterium -mediated plant transformation by mitigating oxidative stress. browning and death of cells transformed with agrobacterium tumefaciens is a long-standing and high impact problem in plant transformation and the agricultural biotechnology industry, severely limiting the production of transgenic plants. using our tomato cv. microtom trans ... | 2015 | 25429877 |
| overexpression of bacterial mtld gene in peanut improves drought tolerance through accumulation of mannitol. | in the changing global environmental scenarios, water scarcity and recurrent drought impose huge reductions to the peanut (arachis hypogaea l.) crop yield. in plants, osmotic adjustments associated with efficient free radical scavenging ability during abiotic stress are important components of stress tolerance mechanisms. mannitol, a compatible solute, is known to scavenge hydroxyl radicals generated during various abiotic stresses, thereby conferring tolerance to water-deficit stress in many pl ... | 2014 | 25436223 |
| development and validation of a p-35s, t-nos, t-35s and p-fmv tetraplex real-time pcr screening method to detect regulatory genes of genetically modified organisms in food. | in routine analysis screening methods based on real-time pcr (polymerase chain reaction) are most commonly used for the detection of genetically modified (gm) plant material in food and feed. screening tests are based on sequences frequently used for gm development, allowing the detection of a large number of gmos (genetically modified organisms). here, we describe the development and validation of a tetraplex real-time pcr screening assay comprising detection systems for the regulatory genes ca ... | 2014 | 25437161 |
| c-terminal domain of cagx is responsible for its interaction with cagt protein of helicobacter pylori type iv secretion system. | helicobacter pylori are the well known human pathogen associated with gastric cancer and peptic ulcer. pathogenesis is mainly due to the presence of 40 kb cagpai (cag pathogenicity island) region that encodes the type iv secretion system (tfss) consisting of a cytoplasmic part, a middle part/core complex (spans from inner membrane to outer membrane), and an outer membrane associated part. cagx and cagt are two important proteins of tfss that have homology with virb9 and virb7 of agrobacterium tu ... | 2015 | 25446105 |
| dc-analyzer-facilitated combinatorial strategy for rapid directed evolution of functional enzymes with multiple mutagenesis sites. | iterative saturation mutagenesis (ism) has been shown to be a powerful method for directed evolution. in this study, the approach was modified (termed m-ism) by combining the single-site saturation mutagenesis method with a dc-analyzer-facilitated combinatorial strategy, aiming to evolve novel biocatalysts efficiently in the case where multiple sites are targeted simultaneously. initially, all target sites were explored individually by constructing single-site saturation mutagenesis libraries. n ... | 2014 | 25449543 |
| ecological dynamics and complex interactions of agrobacterium megaplasmids. | as with many pathogenic bacteria, agrobacterial plant pathogens carry most of their virulence functions on a horizontally transmissible genetic element. the tumor-inducing (ti) plasmid encodes the majority of virulence functions for the crown gall agent agrobacterium tumefaciens. this includes the vir genes which drive genetic transformation of host cells and the catabolic genes needed to utilize the opines produced by infected plants. the ti plasmid also encodes, an opine-dependent quorum sensi ... | 2014 | 25452760 |
| structure and function of a decarboxylating agrobacterium tumefaciens keto-deoxy-d-galactarate dehydratase. | agrobacterium tumefaciens (at) strain c58 contains an oxidative enzyme pathway that can function on both d-glucuronic and d-galacturonic acid. the corresponding gene coding for at keto-deoxy-d-galactarate (kdg) dehydratase is located in the same gene cluster as those coding for uronate dehydrogenase (at udh) and galactarolactone cycloisomerase (at gci) which we have previously characterized. here, we present the kinetic characterization and crystal structure of at kdg dehydratase, which catalyze ... | 2014 | 25454257 |
| phylogenetic multilocus sequence analysis of native rhizobia nodulating faba bean (vicia faba l.) in egypt. | the taxonomic diversity of forty-two rhizobium strains, isolated from nodules of faba bean grown in egypt, was studied using 16s rrna sequencing, multilocus sequence analyses (mlsa) of three chromosomal housekeeping loci and one nodulation gene (noda). based on the 16s rrna gene sequences, most of the strains were related to rhizobium leguminosarum, rhizobium etli, and rhizobium radiobacter (syn. agrobacterium tumefaciens). a maximum likelihood (ml) tree built from the concatenated sequences of ... | 2014 | 25458609 |
| characterisation and microstructure of reduced-fat chicken patties made with a novel polymer from agrobacterium radiobacter k84. | chicken patties elaborated with a novel polymer from agrobacterium radiobacter k84 (arb) were characterised during 60days of frozen storage. after cooking, formulations without arb (f0), with arb 5 g/100 g (f5) and arb 10 g/100 g (f10) presented 4.23%, 2.83% and 0.11% fat, respectively. no differences were observed to water holding capacity, cooking yield and shear force among formulations. microstructural analysis showed formation of meat emulsion for f5 and gel for f10. colour and chicken flav ... | 2015 | 25466137 |
| application of sonication in combination with vacuum infiltration enhances the agrobacterium-mediated genetic transformation in indian soybean cultivars. | soybean is a recalcitrant crop to agrobacterium-mediated genetic transformation. development of highly efficient, reproducible, and genotype-independent transformation protocol is highly desirable for soybean genetic improvement. hence, an improved agrobacterium-mediated genetic transformation protocol has been developed for cultivar pk 416 by evaluating various parameters including agrobacterium tumefaciens strains (lba4404, eha101, and eha105 harboring pcambia1304 plasmid), sonication duration ... | 2015 | 25480345 |
| overexpression of the pumpkin (cucurbita maxima) 16 kda phloem protein cmpp16 increases tolerance to water deficit. | the phloem plays an important role in the delivery of nutrients and signals between photosynthetic to heterotrophic tissues. proteins and rnas in the phloem translocation stream may have an important role in maintaining the integrity of the sieve tube system, as well as in long-distance signaling. cmpp16 is a pumpkin phloem protein, which has been shown to bind rna in a non-sequence specific manner, and move it cell-to-cell and conceivably, long-distance. the protein and rna are found in both co ... | 2014 | 25482781 |
| a rapid and stable agrobacterium-mediated transformation method of a medicinal plant chelone glabra l. | transformation approach is a useful tool for the study of gene function, the mechanism of molecular regulation, and increase usefulness of components by reverse genetic approach in plants. in this study, we developed a stable and rapid method for agrobacterium-mediated transformation of a medicinal plant chelone glabra l. using leaf explants. stable transformants were obtained using agrobacterium tumefaciens strains gv2260 and gv3101 that harbored the binary vector pbi121 and contained the neomy ... | 2015 | 25492686 |
| insights into the polerovirus-plant interactome revealed by coimmunoprecipitation and mass spectrometry. | identification of host proteins interacting with the aphidborne potato leafroll virus (plrv) from the genus polerovirus, family luteoviridae, is a critical step toward understanding how plrv and related viruses infect plants. however, the tight spatial distribution of plrv to phloem tissues poses challenges. a polyclonal antibody raised against purified plrv virions was used to coimmunoprecipitate virus-host protein complexes from nicotiana benthamiana tissue inoculated with an infectious plrv c ... | 2015 | 25496593 |
| an efficient plant regeneration and agrobacterium-mediated genetic transformation of tagetes erecta. | tagetes erecta, l. an asteraceous plant of industrial and medicinal value, contains important compounds like pyrethrins, thiophenes and lutein, possessing immense potential for insecticidal, nematicidal and nutraceutical activities. considering the importance and demand for these natural compounds, genetic manipulation of this crop for better productivity of secondary metabolites holds great significance. a rapid and reproducible direct regeneration and genetic transformation system is the prere ... | 2015 | 25504508 |
| interaction of the agrobacterium tumefaciens virulence protein vird2 with histones. | agrobacterium tumefaciens is a gram-negative soil bacterium that genetically transforms plants and, under laboratory conditions, also transforms non-plant organisms, such as fungi and yeasts. during the transformation process a piece of ssdna (t-strand) is transferred into the host cells via a type iv secretion system. the vird2 relaxase protein, which is covalently attached at the 5' end of the t-strand through tyr29, mediates nuclear entry as it contains a nuclear localization sequence. how th ... | 2015 | 25505187 |
| peritonitis in a peritoneal dialysis patient due to rhizobium radiobacter and moraxella osleonsis: case report and literature review. | 2017 | 25520491 | |
| plant-agrobacterium interaction mediated by ethylene and super-agrobacterium conferring efficient gene transfer. | agrobacterium tumefaciens has a unique ability to transfer genes into plant genomes. this ability has been utilized for plant genetic engineering. however, the efficiency is not sufficient for all plant species. several studies have shown that ethylene decreased the agrobacterium-mediated transformation frequency. thus, a. tumefaciens with an ability to suppress ethylene evolution would increase the efficiency of agrobacterium-mediated transformation. some studies showed that plant growth-promot ... | 2014 | 25520733 |
| effect of agrobacterium strain and plasmid copy number on transformation frequency, event quality and usable event quality in an elite maize cultivar. | improving agrobacterium -mediated transformation frequency and event quality by increasing binary plasmid copy number and appropriate strain selection is reported in an elite maize cultivar. agrobacterium-mediated maize transformation is a well-established method for gene testing and for introducing useful traits in a commercial biotech product pipeline. to develop a highly efficient maize transformation system, we investigated the effect of two agrobacterium tumefaciens strains and three differ ... | 2015 | 25558819 |
| cloning and expression of tnf related apoptosis inducing ligand in nicotiana tabacum. | molecular farming has been considered as a secure and economical approach for production of biopharmaceuticals. human tnf related apoptosis inducing ligand (trail) as a promising biopharmaceutical candidate has been produced in different expression hosts. however, little attention has been paid to molecular farming of the trail in spite of numerous advantages of plant expression systems. therefore, in this study the cytoplasmic production of the trail was tackled in nicotiana tabacum using agrob ... | 2015 | 25561925 |
| isolation and characterization of a secoiridoid derivative from two-phase olive waste (alperujo). | a secoiridoid derivative was isolated from the ethyl acetate extract of two-phase olive waste (alperujo). the structure of this compound was fully characterized as s-trans-(e)-3-(1-oxobut-2-en-2-yl)glutaric acid. the spectroscopic data, including one- and two-dimensional nuclear magnetic resonance, mass spectrometry, infrared analysis, and ultraviolet spectrum, were showed. the origin of this compound has not been previously studied, although it most likely results from the breakdown of the oleu ... | 2015 | 25574580 |
| arsenite oxidase also functions as an antimonite oxidase. | arsenic and antimony are toxic metalloids and are considered priority environmental pollutants by the u.s. environmental protection agency. significant advances have been made in understanding microbe-arsenic interactions and how they influence arsenic redox speciation in the environment. however, even the most basic features of how and why a microorganism detects and reacts to antimony remain poorly understood. previous work with agrobacterium tumefaciens strain 5a concluded that oxidation of a ... | 2015 | 25576601 |
| simple screening strategy with only water bath needed for the identification of insect-resistant genetically modified rice. | an informative, with simple instrument needed, rapid and easily updated strategy for the identification of insect-resistant genetically modified (gm) rice has been described. such strategy is based on a parallel series of loop-mediated isothermal amplification (lamp) reactions targeting the rice endogenous gene sucrose phosphate synthase (sps), the top two most frequently used genetic elements (agrobacterium tumefaciens nopaline synthase terminator (nos) and cauliflower mosaic virus 35s promoter ... | 2015 | 25582220 |
| triesterase and promiscuous diesterase activities of a di-co(ii)-containing organophosphate degrading enzyme reaction mechanisms. | the reaction mechanism for the hydrolysis of trimethyl phosphate and of the obtained phosphodiester by the di-co(ii) derivative of organophosphate degrading enzyme from agrobacterium radiobacter p230(opda), have been investigated at density functional level of theory in the framework of the cluster model approach. both mechanisms proceed by a multistep sequence and each catalytic cycle begins with the nucleophilic attack by a metal-bound hydroxide on the phosphorus atom of the substrate, leading ... | 2015 | 25582757 |
| chemical composition and antibacterial activity of methanolic extract and essential oil of iranian teucrium polium against some of phytobacteria. | the antibacterial activity of essential oil and methanolic extract of teucrium polium was determined against pseudomonas aeruginosa, pantoea agglomerans, brenneria nigrifluens, rhizobium radiobacter, rhizobium vitis, streptomyces scabies, ralstonia solanacearum, xanthomonas campestris and pectobacterium cartovorum by disc diffusion method. minimum inhibitory concentration and minimum bactericidal concentration were determined by using the serial dilution method. chemical composition of essential ... | 2015 | 25583240 |
| the agrobacterium ti plasmids. | agrobacterium tumefaciens is a plant pathogen with the capacity to deliver a segment of oncogenic dna carried on a large plasmid called the tumor-inducing or ti plasmid to susceptible plant cells. a. tumefaciens belongs to the class alphaproteobacteria, whose members include other plant pathogens (agrobacterium rhizogenes), plant and insect symbionts (rhizobium spp. and wolbachia spp., respectively), human pathogens (brucella spp., bartonella spp., rickettsia spp.), and nonpathogens (caulobacter ... | 2014 | 25593788 |
| genomic analyses of metal resistance genes in three plant growth promoting bacteria of legume plants in northwest mine tailings, china. | to better understand the diversity of metal resistance genetic determinant from microbes that survived at metal tailings in northwest of china, a highly elevated level of heavy metal containing region, genomic analyses was conducted using genome sequence of three native metal-resistant plant growth promoting bacteria (pgpb). it shows that: mesorhizobium amorphae ccnwgs0123 contains metal transporters from p-type atpase, cdf (cation diffusion facilitator), hupe/urej and chr (chromate ion transpor ... | 2015 | 25597676 |
| snare-rnai results in higher terpene emission from ectopically expressed caryophyllene synthase in nicotiana benthamiana. | plants produce numerous terpenes and much effort has been dedicated to the identification and characterization of the terpene biosynthetic genes. however, little is known about how terpenes are transported within the cell and from the cell into the apoplast. to investigate a putative role of vesicle fusion in this process, we used agrobacterium tumefaciens-mediated transient coexpression in nicotiana benthamiana of an mtvamp721e-rnai construct (vi) with either a caryophyllene synthase or a linal ... | 2015 | 25598143 |
| enhanced-transient expression of hepatitis c virus core protein in nicotiana tabacum, a protein with potential clinical applications. | hepatitis c virus (hcv) is major cause of liver cirrhosis in humans. hcv capsid (core) protein (hcvcp) is a highly demanded antigen for various diagnostic, immunization and pathogenesis studies. plants are considered as an expression system for producing safe and inexpensive biopharmaceutical proteins. although invention of transgenic (stable) tobacco plants expressing hcvcp with proper antigenic properties was recently reported, no data for "transient-expression" that is currently the method of ... | 2014 | 25598788 |
| the phylogenetically-related pattern recognition receptors efr and xa21 recruit similar immune signaling components in monocots and dicots. | during plant immunity, surface-localized pattern recognition receptors (prrs) recognize pathogen-associated molecular patterns (pamps). the transfer of prrs between plant species is a promising strategy for engineering broad-spectrum disease resistance. thus, there is a great interest in understanding the mechanisms of prr-mediated resistance across different plant species. two well-characterized plant prrs are the leucine-rich repeat receptor kinases (lrr-rks) efr and xa21 from arabidopsis thal ... | 2015 | 25607985 |
| regulation of oncogene expression in t-dna-transformed host plant cells. | virulent agrobacterium tumefaciens strains integrate their t-dna into the plant genome where the encoded agrobacterial oncogenes are expressed and cause crown gall disease. essential for crown gall development are iaah (indole-3-acetamide hydrolase), iaam (tryptophan monooxygenase) and ipt (isopentenyl transferase), which encode enzymes for the biosynthesis of auxin (iaah, iaam) and cytokinin (ipt). although these oncogenes are well studied as the tumor-inducing principle, nothing is known about ... | 2015 | 25615824 |
| progress in genetic engineering of peanut (arachis hypogaea l.)--a review. | peanut (arachis hypogaea l.) is a major species of the family, leguminosae, and economically important not only for vegetable oil but as a source of proteins, minerals and vitamins. it is widely grown in the semi-arid tropics and plays a role in the world agricultural economy. peanut production and productivity is constrained by several biotic (insect pests and diseases) and abiotic (drought, salinity, water logging and temperature aberrations) stresses, as a result of which crop experiences ser ... | 2015 | 25626474 |
| identification of pathogenicity-related genes in the rice pathogen ustilaginoidea virens through random insertional mutagenesis. | agrobacterium tumefaciens-mediated transformation (atmt) is becoming a popular effective system as an insertional mutagenesis tool in filamentous fungi. to gain more insight into the cellular and molecular mechanisms in the pathogenesis of ustilaginoidea virens, the causal agent of rice false smut disease, a t-dna insertion mutant library of u. virens was established using atmt. we optimized a range of conditions to improve the transformation efficiency. transformants were most effectively obtai ... | 2015 | 25636735 |
| comparative whole-genome analyses of selection marker-free rice-based cholera toxin b-subunit vaccine lines and wild-type lines. | we have developed a rice-based oral cholera vaccine named mucorice-ctb (cholera toxin b-subunit) by using an agrobacterium tumefaciens-mediated co-transformation system. to assess the genome-wide effects of this system on the rice genome, we compared the genomes of three selection marker-free mucorice-ctb lines with those of two wild-type rice lines (oryza sativa l. cv. nipponbare). mutation profiles of the transgenic and wild-type genomes were examined by next-generation sequencing (ngs). | 2015 | 25653106 |
| a simple and efficient genetic transformation method of ganoderma weberianum. | in this study, the agrobacterium tumefaciens-mediated transformation method for ganderma weberianum has been established. driven by the cauliflower mosaic virus (camv) 35s promoter, the hygromycin phosphotransferase (hpt), β-glucuronidase (uida), and enhanced green fluorescent protein (egfp) genes have been efficiently expressed in transgenic mycelia and spores. the transformation system was composed of the growing mycelia, a. tumefaciens strain gv3101, and the expression vector pbi-h1, harborin ... | 2015 | 25655754 |
| a single point mutation enhances hydroxynitrile synthesis by halohydrin dehalogenase. | the cyanide-mediated ring opening of epoxides catalyzed by halohydrin dehalogenases yields β-hydroxynitriles that are of high interest for synthetic chemistry. the best studied halohydrin dehalogenase to date is the enzyme from agrobacterium radiobacter, but this enzyme (hhec) exhibits only low cyanolysis activities. sequence comparison between a pair of related halohydrin dehalogenases from corynebacterium and mycobacterium suggested that substitution of a threonine that interacts with the acti ... | 2015 | 25659632 |
| rhizobium pusense is the main human pathogen in the genus agrobacterium/rhizobium. | rhizobium pusense was recently described after isolation from the rhizosphere of chickpea. multilocus sequence-based analysis of clinical isolates identified as agrobacterium (rhizobium) radiobacter demonstrated that r. pusense is the main human pathogen within agrobacterium (rhizobium) spp. clinical microbiology of agrobacterium (rhizobium) should be considered in the light of recent taxonomic changes. | 2015 | 25669878 |
| aboveground insect infestation attenuates belowground agrobacterium-mediated genetic transformation. | agrobacterium tumefaciens causes crown gall disease. although agrobacterium can be popularly used for genetic engineering, the influence of aboveground insect infestation on agrobacterium induced gall formation has not been investigated. nicotiana benthamiana leaves were exposed to a sucking insect (whitefly) infestation and benzothiadiazole (bth) for 7 d, and these exposed plants were inoculated with a tumorigenic agrobacterium strain. we evaluated, both in planta and in vitro, how whitefly inf ... | 2015 | 25676198 |
| exploring the enantioselective mechanism of halohydrin dehalogenase from agrobacterium radiobacter ad1 by iterative saturation mutagenesis. | halohydrin dehalogenase from agrobacterium radiobacter ad1 (hhec) shows great potential in producing valuable chiral epoxides and β-substituted alcohols. the wild-type (wt) enzyme displays a high r-enantiopreference toward most aromatic substrates, whereas no s-selective hhec has been reported to date. to obtain more enantioselective enzymes, seven noncatalytic active-site residues were subjected to iterative saturation mutagenesis (ism). after two rounds of screening aspects of both activity an ... | 2015 | 25681194 |
| optimization of chlorella vulgaris and bioflocculant-producing bacteria co-culture: enhancing microalgae harvesting and lipid content. | microalgae are a sustainable bioresource, and the biofuel they produce is widely considered to be an alternative to limited natural fuel resources. however, microalgae harvesting is a bottleneck in the development of technology. axenic chlorella vulgaris microalgae exhibit poor harvesting, as expressed by a flocculation efficiency of 0·2%. this work optimized the co-culture conditions of c. vulgaris and bioflocculant-producing bacteria in synthetic wastewater using response surface methodology ( ... | 2015 | 25693426 |
| a novel sterol regulatory element-binding protein gene (srea) identified in penicillium digitatum is required for prochloraz resistance, full virulence and erg11 (cyp51) regulation. | penicillium digitatum is the most destructive postharvest pathogen of citrus fruits, causing fruit decay and economic loss. additionally, control of the disease is further complicated by the emergence of drug-resistant strains due to the extensive use of triazole antifungal drugs. in this work, an orthologus gene encoding a putative sterol regulatory element-binding protein (srebp) was identified in the genome of p. digitatum and named srea. the putative srea protein contains a conserved domain ... | 2015 | 25699519 |
| rhizobium metallidurans sp. nov., a symbiotic heavy metal resistant bacterium isolated from the anthyllis vulneraria zn-hyperaccumulator. | a gram-stain-negative, aerobic, rod-shaped, non-spore-forming bacterium (chimec512(t)) was isolated from 56 host seedlings of the hyperaccumulating anthyllis vulneraria legume, which was on an old zinc mining site at les avinières, saint-laurent-le-minier, gard, south of france. on the basis of 16s rrna gene sequence similarities, strain chimec512(t) was shown to belong to the genus rhizobium and to be most closely related to rhizobium endophyticum ccge 2052(t) (98.4%), rhizobium tibeticum ccbau ... | 2015 | 25701848 |
| engineering isoprenoid biosynthesis in artemisia annua l. for the production of taxadiene: a key intermediate of taxol. | taxadiene is the first committed precursor to paclitaxel, marketed as taxol, arguably the most important anticancer agent against ovarian and breast cancer. in taxus, taxadiene is directly synthesized from geranylgeranyl diphosphate (ggpp) that is the common precursor for diterpenoids and is found in most plants and microbes. in this study, artemisia annua l., a chinese medicinal herb that grows fast and is rich in terpenoids, was used as a genetic engineering host to produce taxadiene. the txs ... | 2015 | 25705665 |
| novel pseudotaxis mechanisms improve migration of straight-swimming bacterial mutants through a porous environment. | bacterial locomotion driven by flagella is given directionality by the chemotaxis signal transduction network. in the classic plate assays of migration in porous motility agar, efficient motility is compromised in chemotaxis mutants of diverse bacteria. nonchemotactic mutants become trapped within the agar matrix. suppressor mutations that prevent this entanglement but do not restore chemotaxis, a phenomenon designated pseudotaxis, were first reported to arise for escherichia coli. in this study ... | 2015 | 25714707 |
| plant cell death caused by fungal, bacterial, and viral elicitors: protective effect of mitochondria-targeted quinones. | chitosan (partially deacetylated chitin), a component of fungal cell walls, caused epidermal cell (ec) death in the leaves of pea (pisum sativum l.) and tobacco nicotiana tabacum or nicotiana benthamiana detected by destruction of cell nuclei. the mitochondria-targeted quinone skq1 prevented the destruction of ec nuclei induced by chitosan. chitosan increased and skq1 suppressed the activity of protein kinases in n. benthamiana and p. sativum and eliminated the effect of chitosan. chitosan induc ... | 2014 | 25716725 |
| against friend and foe: type 6 effectors in plant-associated bacteria. | bacterial secretion systems play critical roles in communication with neighboring bacteria and in the modulation of host immune responses via the secretion of small proteins called effectors. several secretion systems have been identified and these are denoted types i-vii. of these, the type vi secretion system (t6ss) and its effectors were only recently elucidated. most studies on the role and significance of the t6ss and its effectors have focused on human pathogens. in this review, type 6 eff ... | 2015 | 25732741 |
| transgene-induced gene silencing in plants. | rnai is the most important reverse genetics tool to trigger transgenic gene silencing, which is now applied widely to investigate gene function and also practically applied to enhance resistance to biotic and abiotic stress. recently, the most effective way to induce transgenic gene silencing is to introduce inverted repeat (ir) double-stranded rna (dsrna) or artificial microrna (amirna) instead of a transgenic sense or antisense strand of genes. the stable transgenic plants can be acquired thro ... | 2015 | 25740359 |
| a high-throughput rna interference (rnai)-based approach using hairy roots for the study of plant-rhizobia interactions. | legumes are major contributors to sustainable agriculture; their key feature is their ability to fix atmospheric nitrogen through symbiotic nitrogen fixation. legumes are often recalcitrant to regeneration and transformation by agrobacterium tumefaciens; however, a. rhizogenes-mediated root transformation and composite plant generation are rapid and convenient alternatives to study root biology, including root nodule symbiosis. rna interference (rnai), coupled with a. rhizogenes-mediated root tr ... | 2015 | 25740364 |
| a functional genomics method for assaying gene function in phytopathogenic fungi through host-induced gene silencing mediated by agroinfiltration. | with the rapid growth of genomic information, there is an increasing demand for efficient analysis tools to study the function of predicted genes coded in genomes. agroinfiltration, the delivery of gene constructs into plant cells by agrobacterium tumefaciens infiltrated into leaves, is one such versatile, simple, and rapid technique that is increasingly used for transient gene expression assay in plants. in this chapter, we focus on the use of agroinfiltration as a functional genomics research ... | 2015 | 25740365 |
| characterization of the highly active fragment of glyceraldehyde-3-phosphate dehydrogenase gene promoter for recombinant protein expression in pleurotus ostreatus. | developing efficient native promoters is important for improving recombinant protein expression by fungal genetic engineering. the promoter region of glyceraldehyde-3-phosphate dehydrogenase gene in pleurotus ostreatus (pogpd) was isolated and optimized by upstream truncation. the activities of these promoters with different lengths were further confirmed by fluorescence, quantitative real-time pcr and western blot analysis. a truncated pogpd-p2 fragment (795 bp) drove enhanced green fluorescenc ... | 2015 | 25743073 |
| molecular and phenotypic characterization of agrobacterium species from vineyards allows identification of typical agrobacterium vitis and atypical biovar 1 strains. | to molecularly and phenotypically characterize a selection of agrobacterium-like isolates from grapevine canes, crowns, soil and tumours in plants grown under cold conditions. | 2015 | 25752332 |
| effects of tung oilseed fad2 and dgat2 genes on unsaturated fatty acid accumulation in rhodotorula glutinis and arabidopsis thaliana. | genetic engineering to produce valuable lipids containing unsaturated fatty acids (ufas) holds great promise for food and industrial applications. efforts to genetically modify plants to produce desirable ufas with single enzymes, however, have had modest success. the key enzymes fatty acid desaturase (fad) and diacylglycerol acyltransferase (dgat) are responsible for ufa biosynthesis (a push process) and assembling fatty acids into lipids (a pull process) in plants, respectively. to examine the ... | 2015 | 25754996 |
| quantitating plant microrna-mediated target repression using a dual-luciferase transient expression system. | microrna (mirna) mediated repression of target genes plays essential roles in a variety of functions in plants. an easy-to-use method that can effectively validate functional mirna-target interactions in plants thus is of particular interest. here, we describe an agrobacterium tumefaciens-mediated in vivo assay utilizing a dual-luciferase reporter system. with this method, the strength of mirna-mediated target repression can be quantified at both the mrna (via qrt-pcr) and protein (via dual-luci ... | 2015 | 25757778 |
| rapid, high-throughput tracking of bacterial motility in 3d via phase-contrast holographic video microscopy. | tracking fast-swimming bacteria in three dimensions can be extremely challenging with current optical techniques and a microscopic approach that can rapidly acquire volumetric information is required. here, we introduce phase-contrast holographic video microscopy as a solution for the simultaneous tracking of multiple fast moving cells in three dimensions. this technique uses interference patterns formed between the scattered and the incident field to infer the three-dimensional (3d) position an ... | 2015 | 25762336 |
| bacillus amyloliquefaciens strain 32a as a source of lipopeptides for biocontrol of agrobacterium tumefaciens strains. | a bacillus amyloliquefaciens strain, designated 32a, was used to identify new compounds active against agrobacterium tumefaciens and to evaluate their efficiency to control crown gall on carrot discs. | 2015 | 25764969 |
| the class iii cyclobutane pyrimidine dimer photolyase structure reveals a new antenna chromophore binding site and alternative photoreduction pathways. | photolyases are proteins with an fad chromophore that repair uv-induced pyrimidine dimers on the dna in a light-dependent manner. the cyclobutane pyrimidine dimer class iii photolyases are structurally unknown but closely related to plant cryptochromes, which serve as blue-light photoreceptors. here we present the crystal structure of a class iii photolyase termed photolyase-related protein a (phra) of agrobacterium tumefaciens at 1.67-å resolution. phra contains 5,10-methenyltetrahydrofolate (m ... | 2015 | 25784552 |
| expression of anti-tumor necrosis factor alpha (tnfα) single-chain variable fragment (scfv) in spirodela punctata plants transformed with agrobacterium tumefaciens. | therapeutic antibodies against tumor necrosis factor alpha (tnfα) have been considered effective for some of the autoimmune diseases such as rheumatoid arthritis, crohn's diseases, and so on. but associated limitations of the current therapeutics in terms of cost, availability, and immunogenicity have necessitated the need for alternative candidates. single-chain variable fragment (scfv) can negate the limitations tagged with the anti-tnfα therapeutics to a greater extent. in the present study, ... | 2016 | 25786575 |
| nicotine-degrading microorganisms and their potential applications. | nicotine-degrading microorganisms (ndms) are a special microbial group which can use nicotine as the sole carbon and nitrogen source for growth. since the 1950s, the bioconversion of nicotine by microbes has received increasing attention, and several ndms have been identified, such as arthrobacter nicotinovorans, microsporum gypseum, pellicularia filamentosa jts-208, and pseudomonas sp. 41. in recent years, increasing numbers of ndms have been isolated and identified from tobacco plantation soil ... | 2015 | 25805341 |
| a fungal transcription factor gene is expressed in plants from its own promoter and improves drought tolerance. | a fungal gene encoding a transcription factor is expressed from its own promoter in arabidopsis phloem and improves drought tolerance by reducing transpiration and increasing osmotic potential. horizontal gene transfer from unrelated organisms has occurred in the course of plant evolution, suggesting that some foreign genes may be useful to plants. the cthsr1 gene, previously isolated from the halophytic yeast candida tropicalis, encodes a heat-shock transcription factor-related protein. cthsr1, ... | 2015 | 25809153 |
| transient plant transformation mediated by agrobacterium tumefaciens: principles, methods and applications. | agrobacterium tumefaciens is widely used as a versatile tool for development of stably transformed model plants and crops. however, the development of agrobacterium based transient plant transformation methods attracted substantial attention in recent years. transient transformation methods offer several applications advancing stable transformations such as rapid and scalable recombinant protein production and in planta functional genomics studies. herein, we highlight agrobacterium and plant ge ... | 2015 | 25819757 |
| comparison and evaluation of two diagnostic methods for detection of npt ii and gus genes in nicotiana tabacum. | to diminish the time required for some diagnostic assays including polymerase chain reaction (pcr), loop-mediated isothermal amplification (lamp) and also a visual detection protocol on the basis of npt ii and gus genes in transgenic tobacco plants were used. agrobacterium tumefaciens-mediated transformation of nicotiana tabacum leaf discs was performed with plant transformation vector of pbi 121. from kanamycin-resistant plants selected by their antibiotic resistance, four plants were selected ... | 2015 | 25820356 |
| effect of leaf incubation temperature profiles on agrobacterium tumefaciens-mediated transient expression. | agrobacterium tumefaciens-mediated transient expression is known to be highly dependent on incubation temperature. compared with early studies that were conducted at constant temperature, we examined the effect of variable leaf incubation temperature on transient expression. as a model system, synthetic endoglucanase (e1) and endoxylanase (xyn10a) genes were transiently expressed in detached whole sunflower leaves via vacuum infiltration for biofuel applications. we found that the kinetics of tr ... | 2015 | 25829353 |
| in vitro regeneration and agrobacterium tumefaciens-mediated genetic transformation in asakura-sanshoo (zanthoxylum piperitum (l.) dc. f. inerme makino) an important medicinal plant. | asakura-sanshoo (zanthoxylum piperitum [l.] dc. f. inerme makino) is an important medicinal plant in east asia. transgenic technique could be applied to improve plant traits and analyze gene function. however, there is no report on regeneration and genetic transformation in asakura-sanshoo. | 2017 | 25829778 |
| [meristematic characteristics of tumors initiated by agrobacterium tumefaciens in pea plants]. | it is known that two key groups of plant hormones--auxins and cytokinins--play an important role in plant tumor development. the formation of agrobacterium-induced tumors results from the horizontal transfer of bacterial oncogenes involved in the biosynthesis of these hormones in the plant genome. the role of transcriptional factors in plant tumor development is poorly investigated. it can be assumed that tumor development associated with abnormal cell proliferation can be controlled by the same ... | 2015 | 25857193 |
| spondylodiscitis due to rhizobium radiobacter. | 2015 | 25868625 | |
| unmasking host and microbial strategies in the agrobacterium-plant defense tango. | coevolutionary forces drive adaptation of both plant-associated microbes and their hosts. eloquently captured in the red queen hypothesis, the complexity of each plant-pathogen relationship reflects escalating adversarial strategies, but also external biotic and abiotic pressures on both partners. innate immune responses are triggered by highly conserved pathogen-associated molecular patterns, or pamps, that are harbingers of microbial presence. upon cell surface receptor-mediated recognition of ... | 2015 | 25873923 |
| [overexpression of laea enhances mevastatin production and reduces sporulation of penicillium citrinum]. | to study the regulation of laea overexpression on mevastatin production and sporulation in penicillium citrinum. | 2014 | 25876329 |
| characterization of uronate dehydrogenases catalysing the initial step in an oxidative pathway. | uronate dehydrogenases catalyse the oxidation of uronic acids to aldaric acids, which represent 'top value-added chemicals' that have the potential to substitute petroleum-derived chemicals. the identification and annotation of three uronate dehydrogenases derived from fulvimarina pelagi htcc2506, streptomyces viridochromogenes dsm 40736 and oceanicola granulosus dsm 15982 via sequence analysis is described. characterization and comparison with two known uronate dehydrogenases in regard to subst ... | 2015 | 25884328 |
| overexpression of a gmcnx1 gene enhanced activity of nitrate reductase and aldehyde oxidase, and boosted mosaic virus resistance in soybean. | molybdenum cofactor (moco) is required for the activities of moco-dependant enzymes. cofactor for nitrate reductase and xanthine dehydrogenase (cnx1) is known to be involved in the biosynthesis of moco in plants. in this work, a soybean (glycine max l.) cnx1 gene (gmcnx1) was transferred into soybean using agrobacterium tumefaciens-mediated transformation method. twenty seven positive transgenic soybean plants were identified by coating leaves with phosphinothricin, bar protein quick dip stick a ... | 2015 | 25886067 |
| current technologies and related issues for mushroom transformation. | mushroom transformation requires a series of experimental steps, including generation of host strains with a desirable selective marker, design of vector dna, removal of host cell wall, introduction of foreign dna across the cell membrane, and integration into host genomic dna or maintenance of an autonomous vector dna inside the host cell. this review introduces limitations and obstacles related to transformation technologies along with possible solutions. current methods for cell wall removal ... | 2015 | 25892908 |
| identification of the genes involved in the fruiting body production and cordycepin formation of cordyceps militaris fungus. | a mutant library of cordyceps militaris was constructed by improved agrobacterium tumefaciens-mediated transformation and screened for degradation features. six mutants with altered characters in in vitro and in vivo fruiting body production, and cordycepin formation were found to contain a single copy t-dna. t-dna flanking sequences of these mutants were identified by thermal asymmetric interlaced-pcr approach. atp-dependent helicase, cytochrome oxidase subunit i and ubiquitin-like activating e ... | 2015 | 25892913 |
| mdc-analyzer-facilitated combinatorial strategy for improving the activity and stability of halohydrin dehalogenase from agrobacterium radiobacter ad1. | halohydrin dehalogenase from agrobacterium radiobacter ad1 (hhec) displays a broad substrate range with high regio- and enantioselectivity of both ring-closure and ring-opening reactions, making the enzyme a useful catalyst for the production of optically pure epoxides and β-substituted alcohols. in this study, we report a novel method using an mdc-analyzer-facilitated combinatorial strategy to improve the activity and stability of hhec by simultaneously randomizing multiple contiguous residues. ... | 2015 | 25896949 |
| the genome of cultivated sweet potato contains agrobacterium t-dnas with expressed genes: an example of a naturally transgenic food crop. | agrobacterium rhizogenes and agrobacterium tumefaciens are plant pathogenic bacteria capable of transferring dna fragments [transfer dna (t-dna)] bearing functional genes into the host plant genome. this naturally occurring mechanism has been adapted by plant biotechnologists to develop genetically modified crops that today are grown on more than 10% of the world's arable land, although their use can result in considerable controversy. while assembling small interfering rnas, or sirnas, of sweet ... | 2015 | 25902487 |
| proteomics and genetics for identification of a bacterial antimonite oxidase in agrobacterium tumefaciens. | antimony (sb) and its compounds are listed by the united states environmental protection agency (usepa, 1979) and the european union (cec, 1976) as a priority pollutant. microbial redox transformations are presumed to be an important part of antimony cycling in nature; however, regulation of these processes and the enzymology involved are unknown. in this study, comparative proteomics and reverse transcriptase-pcr analysis of sb(iii)-oxidizing bacterium agrobacterium tumefaciens gw4 revealed an ... | 2015 | 25909855 |
| new polyphenols from a deep sea spiromastix sp. fungus, and their antibacterial activities. | eleven new polyphenols namely spiromastols a-k (1-11) were isolated from the fermentation broth of a deep sea-derived fungus spiromastix sp. mccc 3a00308. their structures were determined by extensive nmr data and mass spectroscopic analysis in association with chemical conversion. the structures are classified as diphenyl ethers, diphenyl esters and isocoumarin derivatives, while the n-propyl group in the analogues is rarely found in natural products. compounds 1-3 exhibited potent inhibitory e ... | 2015 | 25913707 |
| root-associated bacterial endophytes from ralstonia solanacearum resistant and susceptible tomato cultivars and their pathogen antagonistic effects. | this study was undertaken to assess if the root-associated native bacterial endophytes in tomato have any bearing in governing the host resistance to the wilt pathogen ralstonia solanacearum. internal colonization of roots by bacterial endophytes was confirmed through confocal imaging after syto-9 staining. endophytes were isolated from surface-sterilized roots of 4-weeks-old seedlings of known wilt resistant (r) tomato cultivar arka abha and susceptible (s) cv. arka vikas on nutrient agar after ... | 2015 | 25926818 |
| sonication, vacuum infiltration and thiol compounds enhance the agrobacterium-mediated transformation frequency of withania somnifera (l.) dunal. | in the present study, we have established a stable transformation protocol via agrobacterium tumafacines for the pharmaceutically important withania somnifera. six day-old nodal explants were used for 3 day co-cultivation with agrobacterium tumefaciens strain lba4404 harbouring the vector pcamiba2301. among the different injury treatments, sonication, vacuum infiltration and their combination treatments tested, a vacuum infiltration for 10 min followed by sonication for 10 sec with a. tumefacien ... | 2015 | 25927703 |
| overexpression of 3-deoxy-7-phosphoheptulonate synthase gene from gossypium hirsutum enhances arabidopsis resistance to verticillium wilt. | expression of dhs1 in cotton is induced upon infection by verticillium dahliae , and overexpression of ghdhs1 endows transgenic arabidopsis plants excellent verticillium resistance. verticillium wilt is caused by a soil-borne fungus verticillium dahliae. resistance in most cotton cultivars is either scarce or unavailable, making verticillium wilt a major obstacle in cotton production. here, we identified a 3-deoxy-7-phosphoheptulonate synthase (dhs, ec 4.1.2.15) gene from gossypium hirsutum, nam ... | 2015 | 25929795 |
| genetic transformation of extremophilic fungi acidea extrema and acidothrix acidophila. | intact, growing cells of strongly acidophilic fungi acidea extrema and acidothrix acidophila have been successfully transformed by introduction of heterologous dna fragment (composed of the glyceraldehyde-phosphate-dehydrogenase gene promoter from emericella nidulans, a metallothionein-coding gene asmt1 from amanita strobiliformis and glyceraldehyde-phosphate-dehydrogenase gene terminator from colletotrichum gloeosporioides) with the length of 1690 bp. the transformation procedure was based on t ... | 2015 | 25934267 |
| enhancing t-dna transfer efficiency in barley (hordeum vulgare l.) cells using extracellular cellulose and lectin. | a major limitation of transforming barley tissues by agrobacterium tumefaciens is the low frequency of t-dna transfer due to recalcitrance of barley as a host. the effect of extracellular cellulose and lectin on agrobacterium transformation efficiency was investigated in this study. barley callus cultures were transformed with the agl1 strain containing the vector pbi121 in the presence of 10 mg ml(-1) cellulose or 0.001, 0.05 and 0.1 mg ml(-1) lectin. addition of cellulose significantly (p ≤ 0. ... | 2015 | 25935223 |
| the all-alpha domains of coupling proteins from the agrobacterium tumefaciens virb/vird4 and enterococcus faecalis pcf10-encoded type iv secretion systems confer specificity to binding of cognate dna substrates. | bacterial type iv coupling proteins (t4cps) bind and mediate the delivery of dna substrates through associated type iv secretion systems (t4sss). t4cps consist of a transmembrane domain, a conserved nucleotide-binding domain (nbd), and a sequence-variable helical bundle called the all-alpha domain (aad). in the t4cp structural prototype, plasmid r388-encoded trwb, the nbd assembles as a homohexamer resembling reca and dna ring helicases, and the aad, which sits at the channel entrance of the hom ... | 2015 | 25939830 |
| agrobacterium tumefaciens and interkingdom genetic exchange. | 1989 | 25945402 | |
| cyclic di-gmp acts as a cell cycle oscillator to drive chromosome replication. | fundamental to all living organisms is the capacity to coordinate cell division and cell differentiation to generate appropriate numbers of specialized cells. whereas eukaryotes use cyclins and cyclin-dependent kinases to balance division with cell fate decisions, equivalent regulatory systems have not been described in bacteria. moreover, the mechanisms used by bacteria to tune division in line with developmental programs are poorly understood. here we show that caulobacter crescentus, a bacter ... | 2015 | 25945741 |
| transgenic alfalfa plants expressing the sweetpotato orange gene exhibit enhanced abiotic stress tolerance. | alfalfa (medicago sativa l.), a perennial forage crop with high nutritional content, is widely distributed in various environments worldwide. we recently demonstrated that the sweetpotato orange gene (ibor) is involved in increasing carotenoid accumulation and enhancing resistance to multiple abiotic stresses. in this study, in an effort to improve the nutritional quality and environmental stress tolerance of alfalfa, we transferred the ibor gene into alfalfa (cv. xinjiang daye) under the contro ... | 2015 | 25946429 |
| characterization and comparative analysis of promoters from three plant pararetroviruses associated with dahlia (dahlia variabilis). | two distinct caulimoviruses, dahlia mosaic virus (dmv) and dahlia common mosaic virus (dcmv), and an endogenous plant pararetroviral sequence (dveprs, formerly known as dmv-d10) were reported from dahlia (dahlia spp). promoter elements from these dahlia-associated pararetroviruses were identified and characterized. the tata box, the caat box, the transcription start site, the polyadenylation signal, and regulation factors, characteristic of caulimovirus promoters, were present in each of these p ... | 2015 | 25947569 |
| gene overexpression and rna silencing tools for the genetic manipulation of the s-(+)-abscisic acid producing ascomycete botrytis cinerea. | the phytopathogenic ascomycete botrytis cinerea produces several secondary metabolites that have biotechnical significance and has been particularly used for s-(+)-abscisic acid production at the industrial scale. to manipulate the expression levels of specific secondary metabolite biosynthetic genes of b. cinerea with agrobacterium tumefaciens-mediated transformation system, two expression vectors (pcbh1 and pcbg1 with different selection markers) and one rna silencing vector, pcbsilent1, were ... | 2015 | 25955649 |
| diversity and n-acyl-homoserine lactone production by gammaproteobacteria associated with avicennia marina rhizosphere of south indian mangroves. | the diversity of n-acyl-homoserine lactone (ahl)-producing rhizosphere bacterial community associated with avicennia marina in the mangrove ecosystems of south india was investigated. approximately 800 rhizobacteria were isolated from a. marina, and they were screened for the production of ahl using two biosensors, chromobacterium violaceum cv026 and agrobacterium tumefaciens ntl4 (pzlr4). among the total isolates screened, 7% of the rhizobacteria showed positive induction for ahl signals. the b ... | 2015 | 25956585 |
| expression of enterovirus 71 virus-like particles in transgenic enoki (flammulina velutipes). | no commercial vaccines are currently available for enterovirus 71 (ev71) infection. oral virus-like particle (vlp) vaccines are regarded as a better choice for prevention from food-borne diseases compared with injected whole virus vaccines. unfortunately, the application of oral vlp vaccines produced from transgenic plants was limited due to the concerns of gene contamination. alternatively, using transgenic mushrooms retains the advantages of transgenic plants and tremendously reduce risks of g ... | 2015 | 25957149 |
| efficient shoots regeneration and genetic transformation of bacopa monniera. | bacopa monniera is an important source of metabolites with pharmaceutical value. it has been regarded as a valuable medicinal plant and its entire commercial requirement is met from wild natural population. recently, metabolic engineering has emerged as an important solution for sustained supply of assured and quality raw material for the production of active principles. present report describes efficient in vitro multiplication and transformation method for genetic manipulation of this species. ... | 2015 | 25964718 |
| optimization of translation profiles enhances protein expression and solubility. | mrna is translated with a non-uniform speed that actively coordinates co-translational folding of protein domains. using structure-based homology we identified the structural domains in epoxide hydrolases (ehs) and introduced slow-translating codons to delineate the translation of single domains. these changes in translation speed dramatically improved the solubility of two ehs of metagenomic origin in escherichia coli. conversely, the importance of transient attenuation for the folding, and con ... | 2015 | 25965266 |
| [opine biosynthesis and catabolism genes of agrobacterium tumefaciens and agrobacterium rhizogenes]. | agrobacterium is a genus of soil bacteria with the ability to transform plant cells by a t-dna-sequence located on the pti/pri- plasmid containing a set of genes expressed in plant cells. expression of these genes leads to a proliferation of transformed cells, with the subsequent formation of tumors or growths of roots and the synthesis of opines--products of the condensation of amino acids with ketoacids or sugars used by agrobacteria as a source of carbon and nitrogen. in this review, we syste ... | 2015 | 25966579 |
| better rooting procedure to enhance survival rate of field grown malaysian eksotika papaya transformed with 1-aminocyclopropane-1-carboxylic acid oxidase gene. | a high survival rate for transformed papaya plants when transferred to the field is useful in the quest for improving the commercial quality traits. we report in this paper an improved rooting method for the production of transformed malaysian eksotika papaya with high survival rate when transferred to the field. shoots were regenerated from embryogenic calli transformed with antisense and rnai constructs of 1-aminocyclopropane-1-carboxylic acid oxidase (aco) genes using the agrobacterium tumefa ... | 2013 | 25969786 |