Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| development of combined dry heat and chlorine dioxide gas treatment with mechanical mixing for inactivation of salmonella enterica serovar montevideo on mung bean seeds. | foodborne outbreaks have been associated with the consumption of fresh sprouted beans. the sprouting conditions of mung bean seeds provide optimal conditions of temperature and relative humidity for any potential pathogenic contaminant on the seeds to grow. the lack of a kill step postsprouting is a major safety concern. thus, the use of a kill step on the seeds prior to a sprouting step would enhance the safety of fresh sprouts. the objective of this work was to evaluate the effectiveness of th ... | 2015 | 25951379 |
| multiplex pcr-based serogrouping and serotyping of salmonella enterica from tonsil and jejunum with jejunal lymph nodes of slaughtered swine in metro manila, philippines. | food poisoning outbreaks and livestock mortalities caused by salmonella enterica are widespread in the philippines, with hogs being the most commonly recognized carriers of the pathogen. to prevent and control the occurrence of s. enterica infection in the country, methods were used in this study to isolate and rapidly detect, differentiate, and characterize s. enterica in tonsils and jejuna with jejunal lymph nodes of swine slaughtered in four locally registered meat establishments (lrmes) and ... | 2015 | 25951380 |
| salmonella in raw chicken meat from the romanian seaside: frequency of isolation and antibiotic resistance. | the purpose of this study was to investigate the occurrence of salmonella in raw chicken meat samples collected at the romanian seaside and to evaluate the antimicrobial susceptibility of the isolates. in 2012, 317 chicken meat samples from slaughterhouses (n = 289) and retail markets (n = 28) were evaluated. overall, 13.2% (42) of the samples contained salmonella; 12.8% (37) and 17.8% (5) from the chicken carcasses at slaughterhouses and the fresh meat from retail markets, respectively. eight s ... | 2015 | 25951397 |
| genome sequence of salmonella enterica phage det7. | det7 is a myoviridae bacteriophage that gains entry into its salmonella enterica serovar typhimurium host cells by adsorbing to o-antigen polysaccharide. we report here the complete 157,498-bp sequence of its genome. det7, together with its vi01-like relatives, are distantly related to phage t4. | 2015 | 25953168 |
| a handheld flow genetic analysis system (fgas): towards rapid, sensitive, quantitative and multiplex molecular diagnosis at the point-of-care level. | a handheld flow genetic analysis system (fgas) is proposed for rapid, sensitive, multiplex and real-time quantification of nucleic acids at the point-of-care (poc) level. the fgas includes a helical thermal-gradient microreactor and a microflow actuator, as well as control circuitry for temperature, fluid and power management, and smartphone fluorescence imaging. all of these features are integrated into a field-portable and easy-to-use molecular diagnostic platform powered by lithium batteries. ... | 2015 | 25953325 |
| parallel evolutionary pathways to antibiotic resistance selected by biocide exposure. | biocides are widely used to prevent infection. we aimed to determine whether exposure of salmonella to various biocides could act as a driver of antibiotic resistance. | 2015 | 25953808 |
| older leaves of lettuce (lactuca spp.) support higher levels of salmonella enterica ser. senftenberg attachment and show greater variation between plant accessions than do younger leaves. | salmonella can bind to the leaves of salad crops including lettuce and survive for commercially relevant periods. previous studies have shown that younger leaves are more susceptible to colonization than older leaves and that colonization levels are dependent on both the bacterial serovar and the lettuce cultivar. in this study, we investigated the ability of two lactuca sativa cultivars (saladin and iceberg) and an accession of wild lettuce (l. serriola) to support attachment of salmonella ente ... | 2015 | 25953858 |
| sexually acquired salmonella typhi urinary tract infection. | we report a case of isolated urinary salmonella enterica serotype typhi in an hiv-positive man who has sex with men. he was clinically well and blood and stool cultures were negative, indicating that this may have been a sexually acquired urinary tract infection. | 2016 | 25953964 |
| biochemical and kinetic characterization of the eukaryotic phosphotransacetylase class iia enzyme from phytophthora ramorum. | phosphotransacetylase (pta), a key enzyme in bacterial metabolism, catalyzes the reversible transfer of an acetyl group from acetyl phosphate to coenzyme a (coa) to produce acetyl-coa and pi. two classes of pta have been identified based on the absence (pta(i)) or presence (pta(ii)) of an n-terminal regulatory domain. pta(i) has been fairly well studied in bacteria and one genus of archaea; however, only the escherichia coli and salmonella enterica pta(ii) enzymes have been biochemically charact ... | 2015 | 25956919 |
| mechanisms of antimicrobial resistant salmonella enterica transmission associated with starling-livestock interactions. | bird-livestock interactions have been implicated as potential sources for bacteria within concentrated animal feeding operations (cafo). european starlings (sturnus vulgaris) in particular are known to contaminate cattle feed and water with salmonella enterica through their fecal waste. we propose that fecal waste is not the only mechanisms through which starlings introduce s. enterica to cafo. the goal of this study was to assess if starlings can mechanically move s. enterica. we define mechani ... | 2015 | 25960334 |
| phylogeographical analysis of the dominant multidrug-resistant h58 clade of salmonella typhi identifies inter- and intracontinental transmission events. | the emergence of multidrug-resistant (mdr) typhoid is a major global health threat affecting many countries where the disease is endemic. here whole-genome sequence analysis of 1,832 salmonella enterica serovar typhi (s. typhi) identifies a single dominant mdr lineage, h58, that has emerged and spread throughout asia and africa over the last 30 years. our analysis identifies numerous transmissions of h58, including multiple transfers from asia to africa and an ongoing, unrecognized mdr epidemic ... | 2015 | 25961941 |
| in salmonella enterica, ethanolamine utilization is repressed by 1,2-propanediol to prevent detrimental mixing of components of two different bacterial microcompartments. | bacterial microcompartments (mcps) are a diverse family of protein-based organelles composed of metabolic enzymes encapsulated within a protein shell. the function of bacterial mcps is to optimize metabolic pathways by confining toxic and/or volatile metabolic intermediates. about 20% of bacteria produce mcps, and there are at least seven different types. different mcps vary in their encapsulated enzymes, but all have outer shells composed of highly conserved proteins containing bacterial microc ... | 2015 | 25962913 |
| the pdul phosphotransacylase is used to recycle coenzyme a within the pdu microcompartment. | in salmonella enterica, 1,2-propanediol (1,2-pd) utilization (pdu) is mediated by a bacterial microcompartment (mcp). the pdu mcp consists of a multiprotein shell that encapsulates enzymes and cofactors for 1,2-pd catabolism, and its role is to sequester a reactive intermediate (propionaldehyde) to minimize cellular toxicity and dna damage. for the pdu mcp to function, the enzymes encapsulated within must be provided with a steady supply of substrates and cofactors. in the present study, western ... | 2015 | 25962918 |
| outbreak of salmonella enterica serovar typhimurium phage type dt41 in danish poultry production. | salmonella enterica subspecies enterica serovar typhimurium (s. typhimurium) is one of the most prevalent serovars in europe - where both poultry and poultry related products are common sources of human salmonellosis. due to efficient control programs, the prevalence of s. typhimurium in danish poultry production is very low. despite this, during the past decades there has been a reoccurring problem with infections with s. typhimurium phage type dt41 in the danish poultry production without iden ... | 2015 | 25962983 |
| antimicrobial susceptibility of travel-related salmonella enterica serovar typhi isolates detected in switzerland (2002-2013) and molecular characterization of quinolone resistant isolates. | typhoid fever is an acute, invasive, and potentially fatal systemic infection caused by salmonella enterica subspecies enterica serotype typhi (s. typhi). drug resistance to antimicrobials such as ciprofloxacin is emerging in developing countries, threatening the efficacy of treatment of patients in endemic regions as well as of travellers returning from these countries. | 2015 | 25963025 |
| trends in antibiotic susceptibility of enteric fever isolates in east london. | the study sought evidence for changes in the proportions of antibiotic resistant strains among isolates of salmonella enterica serovar typhi (s. typhi) and salmonella enterica serovar paratyphi (s. paratyphi) between 2005 and 2012. | 2015 | 25964222 |
| interaction of salmonella enterica serovar typhimurium with intestinal organoids derived from human induced pluripotent stem cells. | the intestinal mucosa forms the first line of defense against infections mediated by enteric pathogens such as salmonellae. here we exploited intestinal "organoids" (ihos) generated from human induced pluripotent stem cells (hipscs) to explore the interaction of salmonella enterica serovar typhimurium with ihos. imaging and rna sequencing were used to analyze these interactions, and clear changes in transcriptional signatures were detected, including altered patterns of cytokine expression after ... | 2015 | 25964470 |
| a new repertoire of informations about the quorum sensing system in salmonella enterica serovar enteritidis pt4. | salmonella spp are among the main causative agents of foodborne diseases. some phenotypes associated with increased drug resistance and virulence are regulated by quorum sensing (qs). in the present study, the autoinducer (ai)-1- and -2-mediated qs mechanisms were characterized in salmonella enterica serovar enteritidis pt4 for the first time. salmonella enteritidis did not produce ai-1. phylogenetic analysis of nucleotides encoding the sdia protein, the response regulator of ai-1-mediated qs, a ... | 2015 | 25966179 |
| a novel microbial source tracking microarray for pathogen detection and fecal source identification in environmental systems. | pathogen detection and the identification of fecal contamination sources are challenging in environmental waters. factors including pathogen diversity and ubiquity of fecal indicator bacteria hamper risk assessment and remediation of contamination sources. a custom microarray targeting pathogens (viruses, bacteria, protozoa), microbial source tracking (mst) markers, and antibiotic resistance genes was tested against dna obtained from whole genome amplification (wga) of rna and dna from sewage an ... | 2015 | 25970344 |
| host cell type-dependent translocation and phop-mediated positive regulation of the effector ssek1 of salmonella enterica. | salmonella enterica expresses two virulence-related type iii secretion systems (t3sss) encoded in salmonella pathogenicity island 1 (spi1) and spi2, respectively. ssek1 is a poorly characterized substrate of the spi2-encoded t3ss. here, we show that this effector is essential to get full virulence both in oral and intraperitoneal mice infections, in spite of not having a role in invasion or intracellular proliferation in cultured mammalian cells. in vitro, expression of ssek1 was higher in media ... | 2015 | 25972862 |
| mathematical model of flagella gene expression dynamics in salmonella enterica serovar typhimurium. | flagellar assembly in salmonella is controlled by an intricate genetic and biochemical network. this network comprises of a number of inter-connected feedback loops, which control the assembly process dynamically. critical among these are the flia-flgm feedback, fliz-mediated positive feedback, and flit-mediated negative feedback. in this work, we develop a mathematical model to track the dynamics of flagellar gene expression in salmonella. analysis of our model demonstrates that the network is ... | 2015 | 25972986 |
| multidrug-resistant salmonella enterica serovar typhimurium monophasic variant 4,12:i:- isolated from asymptomatic wildlife in a catalonian wildlife rehabilitation center, spain. | wildlife can act as long-term asymptomatic reservoirs for zoonotic bacteria, such as salmonella. the prevalence and antimicrobial-susceptibility profiles of salmonella spp. were assessed in 263 cases in wildlife from 22 animal orders from a wildlife rehabilitation center in catalonia (ne spain), september 2013-may 2014. eleven of 263 tested animals were positive for salmonella spp., representing an overall prevalence of 4.2%. prevalences by taxonomic categories were 2% in mammals, 4.7% in birds, ... | 2015 | 25973627 |
| preliminary incidence and trends of infection with pathogens transmitted commonly through food - foodborne diseases active surveillance network, 10 u.s. sites, 2006-2014. | foodborne illnesses represent a substantial, yet largely preventable, health burden in the united states. in 10 u.s. geographic areas, the foodborne diseases active surveillance network (foodnet) monitors the incidence of laboratory-confirmed infections caused by nine pathogens transmitted commonly through food. this report summarizes preliminary 2014 data and describes changes in incidence compared with 2006-2008 and 2011-2013. in 2014, foodnet reported 19,542 infections, 4,445 hospitalizations ... | 2015 | 25974634 |
| genomic and experimental evidence for multiple metabolic functions in the rida/yjgf/yer057c/uk114 (rid) protein family. | it is now recognized that enzymatic or chemical side-reactions can convert normal metabolites to useless or toxic ones and that a suite of enzymes exists to mitigate such metabolite damage. examples are the reactive imine/enamine intermediates produced by threonine dehydratase, which damage the pyridoxal 5'-phosphate cofactor of various enzymes causing inactivation. this damage is pre-empted by rida proteins, which hydrolyze the imines before they do harm. rida proteins belong to the yjgf/yer057 ... | 2015 | 25975565 |
| absence of multidrug resistance in salmonella enterica serotypes typhi and paratyphi a isolates with intermediate susceptibility to ciprofloxacin. | we describe the antimicrobial susceptibility pattern of 64 blood stream isolates of salmonella enterica serotypes typhi and paratyphi a studied from january 2013 to december 2014 at a tertiary care centre in north india. | 2015 | 25979527 |
| high-throughput crispr typing of mycobacterium tuberculosis complex and salmonella enterica serotype typhimurium. | spoligotyping was developed almost 18 years ago and still remains a popular first-lane genotyping technique to identify and subtype mycobacterium tuberculosis complex (mtc) clinical isolates at a phylogeographic level. for other pathogens, such as salmonella enterica, recent studies suggest that specifically designed spoligotyping techniques could be interesting for public health purposes. spoligotyping was in its original format a reverse line-blot hybridization method using capture probes desi ... | 2015 | 25981468 |
| catecholate siderophore esterases fes, irod and iroe are required for salmochelins secretion following utilization, but only irod contributes to virulence of extra-intestinal pathogenic escherichia coli. | salmochelins are glucosylated forms of enterobactin (enterochelin) and contribute to the virulence of salmonella enterica and some extra-intestinal pathogenic escherichia coli (expec). fes, irod and iroe esterases degrade salmochelins and enterobactin to release iron. we investigated the apparently redundant role of these esterases in virulence and in salmochelin production and utilization of the expec strain χ7122. the δirod, δfesδirod and δfesδirodδiroe mutants displayed attenuated virulence p ... | 2015 | 25982934 |
| editorial on plants as alternative hosts for human and animal pathogens. | 2015 | 25983729 | |
| importance of sigma factor mutations in increased triclosan resistance in salmonella typhimurium. | salmonella enterica is the second most common foodborne pathogen. the use of biocides is crucial to prevent spread of foodborne pathogens, and it would be devastating for food safety if salmonella would become resistant to the disinfectants used. another concern is that exposure to disinfectants might lead to decreased susceptibility to antibiotics. the current study aimed to identify genetic changes causing high level triclosan resistance in s. enterica serovar typhimurium and evaluate how thes ... | 2015 | 25986727 |
| salmonella enterica serotype enteritidis in french polynesia, south pacific, 2008-2013. | outbreaks of salmonella enterica serotype enteritidis infections associated with eggs occurred in french polynesia during 2008-2013. molecular analysis of isolates by using clustered regularly interspaced short palindromic repeat polymorphisms and multilocus variable-number tandem-repeat analysis was performed. this subtyping made defining the epidemic strain, finding the source, and decontaminating affected poultry flocks possible. | 2015 | 25988406 |
| salmonella enterica paratyphi a infections in travelers returning from cambodia, united states. | 2015 | 25988984 | |
| pharmacokinetics of enrofloxacin and ceftiofur in plasma, interstitial fluid, and gastrointestinal tract of calves after subcutaneous injection, and bactericidal impacts on representative enteric bacteria. | this study's objectives were to determine intestinal antimicrobial concentrations in calves administered enrofloxacin or ceftiofur sodium subcutaneously, and their impact on representative enteric bacteria. ultrafiltration devices were implanted in the ileum and colon of 12 steers, which received either enrofloxacin or ceftiofur sodium. samples were collected over 48 h after drug administration for pharmacokinetic/pharmacodynamic analysis. enterococcus faecalis or salmonella enterica (5 × 10(5) ... | 2016 | 25989138 |
| virulence gene regulation by l-arabinose in salmonella enterica. | invasion of the intestinal epithelium is a critical step in salmonella enterica infection and requires functions encoded in the gene cluster known as salmonella pathogenicity island 1 (spi-1). expression of spi-1 genes is repressed by l-arabinose, and not by other pentoses. transport of l-arabinose is necessary to repress spi-1; however, repression is independent of l-arabinose metabolism and of the l-arabinose-responsive regulator arac. spi-1 repression by l-arabinose is exerted at a single tar ... | 2015 | 25991823 |
| a multistate investigation of antibiotic-resistant salmonella enterica serotype i 4,[5],12:i:- infections as part of an international outbreak associated with frozen feeder rodents. | while most human salmonella infections result from exposure to contaminated foods, an estimated 11% of all salmonella infections are attributed to animal exposures, including both direct animal handling and indirect exposures such as cleaning cages and handling contaminated pet food. this report describes the epidemiologic, environmental and laboratory investigations conducted in the united states as part of the response to an international outbreak of tetracycline-resistant salmonella enterica ... | 2016 | 25996458 |
| inactivation of escherichia coli o157:h7 and salmonella enterica on blueberries in water using ultraviolet light. | ultraviolet light (uv) has antimicrobial effects, but the shadowing effect has limited its application. in this study, a novel setup using uv processing in agitated water was developed to inactivate escherichia coli o157:h7 and salmonella on blueberries. blueberries were dip- or spot-inoculated with e. coli or salmonella. blueberries inoculated with e. coli were treated for 2 to 10 min with uv directly (dry uv) or immersed in agitated water during uv treatment (wet uv). e. coli was most easily k ... | 2015 | 25998253 |
| inactivation dynamics of salmonella enterica, listeria monocytogenes, and escherichia coli o157:h7 in wash water during simulated chlorine depletion and replenishment processes. | maintaining effective sanitizer concentration is of critical importance for preventing pathogen survival and transference during fresh-cut produce wash operation and for ensuring the safety of finished products. however, maintaining an adequate level of sanitizer in wash water can be challenging for processors due to the large organic load in the wash system. in this study, we investigated how the survival of human pathogens was affected by the dynamic changes in water quality during chlorine de ... | 2015 | 25998820 |
| salmonella infection inhibits intestinal biotin transport: cellular and molecular mechanisms. | infection with the nontyphoidal salmonella is a common cause of food-borne disease that leads to acute gastroenteritis/diarrhea. severe/prolonged cases of salmonella infection could also impact host nutritional status, but little is known about its effect on intestinal absorption of vitamins, including biotin. we examined the effect of salmonella enterica serovar typhimurium (s. typhimurium) infection on intestinal biotin uptake using in vivo (streptomycin-pretreated mice) and in vitro [mouse (y ... | 2015 | 25999427 |
| effectiveness of levulinic acid and sodium dodecyl sulfate employed as a sanitizer during harvest or packing of cantaloupes contaminated with salmonella poona. | freshly harvested eastern variety cantaloupes (cucumis melo l. var. reticulatus cv. athena) were subjected to three different harvest and wash treatments to examine conditions under which the efficacy of the sanitizer, levulinic acid (lv) plus sodium dodecyl sulfate (sds), could be enhanced to reduce salmonella contamination. in treatment set one, cantaloupes were spot inoculated with salmonella enterica serovar poona (prepared from solid or liquid media cultures) before or after a 1-min dip tre ... | 2015 | 26001062 |
| altered host immune responses to membrane vesicles from salmonella and gram-negative pathogens. | membrane vesicles (mvs), discrete nano-structures produced from the outer membrane of gram-negative bacteria such as salmonella enterica typhimurium (s. typhimurium), strongly activate dendritic cells (dcs), contain major antigens (ags) recognized by salmonella-specific b-cells and cd4+ t-cells, and provide protection against s. typhimurium challenge in a mouse model. with this in mind, we hypothesized that alterations to the gene expression profile of bacteria will be reflected in the immunolog ... | 2015 | 26001432 |
| acidic ph and divalent cation sensing by phoq are dispensable for systemic salmonellae virulence. | salmonella phoq is a histidine kinase with a periplasmic sensor domain (pd) that promotes virulence by detecting the macrophage phagosome. phoq activity is repressed by divalent cations and induced in environments of acidic ph, limited divalent cations, and cationic antimicrobial peptides (camp). previously, it was unclear which signals are sensed by salmonellae to promote phoq-mediated virulence. we defined conformational changes produced in the phoq pd on exposure to acidic ph that indicate st ... | 2015 | 26002083 |
| detection of cell surface hydrophobicity, biofilm and fimbirae genes in salmonella isolated from tunisian clinical and poultry meat. | the aim of this study was to evaluate the ability of 15 serotypes of salmonella to form biofilm on polystyrene, polyvinyl chloride (pvc) and glass surfaces. . | 2014 | 26005652 |
| efficacy of fumigation with trans-cinnamaldehyde and eugenol in reducing salmonella enterica serovar enteritidis on embryonated egg shells. | this study investigated the efficacy of two gras (generally regarded as safe)-status, plant-derived antimicrobials (pdas), namely trans-cinnamaldehyde (tc) and eugenol (eug) applied as a fumigation treatment in reducing se on embryonated egg shells. egg shells of day-old embryonated eggs were spot inoculated with a 4-strain mixture of se (∼6.5 log cfu/egg) and subjected to fumigation with the aforementioned pdas (0 or 1% concentration) for 20 minutes in a hatching incubator. se on the shell and ... | 2015 | 26009758 |
| [salmonella meningitis in an infant due to a pet turtle]. | in humans, salmonella most often causes self-limiting gastroenteritis, but more severe symptoms such as sepsis and meningitis can also occur and can sometimes have a fatal outcome. even if the meningitis is not fatal, sequelae such as epilepsy, cranial nerve palsies, and hydrocephalus can occur. in the united states, it has been estimated that approximately 6% of the human cases of salmonellosis can be attributed to contact with reptiles or amphibians. the infection may take place by direct cont ... | 2015 | 26014646 |
| dissemination of inci2 plasmids that harbor the blactx-m element among clinical salmonella isolates. | the extended-spectrum-β-lactamase (esbl) determinant ctx-m-55 is increasingly prevalent in escherichia coli but remains extremely rare in salmonella. this study reports the isolation of a plasmid harboring the blactx-m-55 element in a clinical salmonella enterica serotype typhimurium strain resistant to multiple antibiotics. this plasmid is genetically identical to several known inci2-type elements harbored by e. coli strains recovered from animals. this finding indicates that inci2 plasmids har ... | 2015 | 26014934 |
| hamp domain rotation and tilting movements associated with signal transduction in the phoq sensor kinase. | hamp domains are α-helical coiled coils that often transduce signals from extracytoplasmic sensing domains to cytoplasmic domains. limited structural information has resulted in hypotheses that specific hamp helix movement changes downstream enzymatic activity. these hypotheses were tested by mutagenesis and cysteine cross-linking analysis of the phoq histidine kinase, essential for resistance to antimicrobial peptides in a variety of enteric pathogens. these results support a mechanistic model ... | 2015 | 26015499 |
| massive lineage replacements and cryptic outbreaks of salmonella typhi in eastern and southern africa. | a new large-scale study reports the whole-genome sequences of nearly 2,000 salmonella enterica serovar typhi (s. typhi) strains collected from 63 countries. a recent and dominant multidrug-resistant (mdr) lineage originating from south asia, which is supplanting a bulk of ancestral drug-sensitive strains, is identified; the success of this lineage is likely driven by plasmid acquisitions and the chromosomal integration of resistance-conferring genes. | 2015 | 26018894 |
| defining the core genome of salmonella enterica serovar typhimurium for genomic surveillance and epidemiological typing. | salmonella enterica serovar typhimurium is the most common salmonella serovar causing foodborne infections in australia and many other countries. twenty-one s. typhimurium strains from salmonella reference collection a (sara) were analyzed using illumina high-throughput genome sequencing. single nucleotide polymorphisms (snps) in 21 sara strains ranged from 46 to 11,916 snps, with an average of 1,577 snps per strain. together with 47 strains selected from publicly available s. typhimurium genome ... | 2015 | 26019201 |
| salmonella enterica biofilm-mediated dispersal by nitric oxide donors in association with cellulose nanocrystal hydrogels. | protected by extracellular polymers, microbes within biofilms are significantly more resistant to disinfectants. current research has been instrumental in identifying nitric oxide donors and hydrogels as potential disinfectant additives. nitric oxide (no) donors are considered a very promising molecule as biofilm dispersal agents and hydrogels have recently attracted a lot of interest due to their biocompatible properties and ability to form stable thin films. when the no donor mahma nonoate was ... | 2015 | 26020015 |
| a series of medium and high copy number arabinose-inducible escherichia coli expression vectors compatible with pbr322 and pacyc184. | the original pbad24 plasmid and the derived lower copy number (the pbad322 series) expression vectors have been widely used in escherichia coli, salmonella enterica, and related bacteria. however, a flexible pbad expression system has been available only in pmb1 (cole1) vectors. we report a series of pbad vectors that replicate using the origin of plasmid rsf1030 that are compatible with pmb1 (cole1) and p15a (pacyc) vectors. both high (≥pbad24) and medium (~pbad322) copy number plasmids encodin ... | 2015 | 26021570 |
| efficient suppression of biofilm formation by a nucleic acid aptamer. | biofilms are microbial communities that are attached to a solid surface using extracellular polymeric substances. motility and initial attachment mediated by flagella are required for biofilm formation. therefore, blocking the motility of flagella is a potential strategy to inhibit biofilm formation. in this study, single-stranded dna aptamers specific to the salmonella choleraesuis were selected after 14 cycles of the systematic evolution of ligands by exponential enrichment. among the selected ... | 2015 | 26025307 |
| rapid draft sequencing and real-time nanopore sequencing in a hospital outbreak of salmonella. | foodborne outbreaks of salmonella remain a pressing public health concern. we recently detected a large outbreak of salmonella enterica serovar enteritidis phage type 14b affecting more than 30 patients in our hospital. this outbreak was linked to community, national and european-wide cases. hospital patients with salmonella are at high risk, and require a rapid response. we initially investigated this outbreak by whole-genome sequencing using a novel rapid protocol on the illumina miseq; we the ... | 2015 | 26025440 |
| sufc may promote the survival of salmonella enterica serovar typhi in macrophages. | the sufc gene of escherichia coli (e. coli) is required for the biogenesis of iron-sulfur (fe-s) cluster under oxidative stress conditions. in order to investigate the roles of sufc in salmonella enterica serovar typhi (s. typhi), isogenic s. typhi strain gifu10007 harboring a non-polar mutation of sufc (δsufc) was constructed and the results showed that the sufc deleted mutant grew more slowly than the wild type strain when encounter oxidative stresses. moreover, the deletion of sufc gene decre ... | 2015 | 26026834 |
| microbiological survey of birds of prey pellets. | a microbiological survey of 73 pellets collected from different birds of prey species housed at the wildlife rescue and rehabilitation center of napoli (southern italy) was performed. pellets were analyzed by culture and biochemical methods as well as by serotyping and polymerase chain reaction. we isolated a wide range of bacteria some of them also pathogens for humans (i.e. salmonella enterica serotype typhimurium, campylobacter coli, escherichia coli o serogroups). this study highlights the p ... | 2015 | 26026881 |
| salmonella manipulation of host signaling pathways provokes cellular transformation associated with gallbladder carcinoma. | cancer is fueled by deregulation of signaling pathways in control of cellular growth and proliferation. these pathways are also targeted by infectious pathogens en route to establishing infection. gallbladder carcinoma (gbc) is frequent in the indian subcontinent, with chronic salmonella enterica serovar typhi infection reported as a significant risk factor. however, direct association and causal mechanisms between salmonella typhi infection and gbc have not been established. deconstructing the ... | 2015 | 26028364 |
| genetic and phenotypic evidence of the salmonella enterica serotype enteritidis human-animal interface in chile. | salmonella enterica serotype enteritidis is a worldwide zoonotic agent that has been recognized as a very important food-borne bacterial pathogen, mainly associated with consumption of poultry products. the aim of this work was to determine genotypic and phenotypic evidence of s. enteritidis transmission among seabirds, poultry and humans in chile. genotyping was performed using pcr-based virulotyping, pulse-field gel electrophoresis (pfge) and multi-locus sequence typing (mlst). pathogenicity-a ... | 2015 | 26029196 |
| burkholderia cenocepacia and salmonella enterica arnt proteins that transfer 4-amino-4-deoxy-l-arabinose to lipopolysaccharide share membrane topology and functional amino acids. | we recently demonstrated that incorporation of 4-amino-4-deoxy-l-arabinose (l-ara4n) to the lipid a moiety of lipopolysaccharide (lps) is required for transport of lps to the outer membrane and viability of the gram-negative bacterium burkholderia cenocepacia. arnt is a membrane protein catalyzing the transfer of l-ara4n to the lps molecule at the periplasmic face of the inner membrane, but its topology and mechanism of action are not well characterized. here, we elucidate the topology of arnt a ... | 2015 | 26030265 |
| synergistic effect of slightly acidic electrolyzed water and ultrasound at mild heat temperature in microbial reduction and shelf-life extension of fresh-cut bell pepper. | the objectives of this study were to evaluate the effect of combined treatments (slightly acidic electrolyzed water (saew), ultrasound (us), or mild heat (60°c)) on the growth of listeria monocytogenes and salmonella enterica serovar typhimurium in fresh-cut bell pepper, and the shelf-life and sensory quality (color and texture) were followed during storage at 4°c and 25°c. an additional 0.65, 1.72, and 2.70 log cfu/g reduction was achieved by heat treatments at 60°c for 1 min for dw, saew, and ... | 2015 | 26032362 |
| salmonella type iii effector sopb modulates host cell exocytosis. | salmonella enterica pathogenesis is dependent on its ability to enter and replicate inside host cells. replication occurs inside the salmonella-containing vacuole (scv), a vacuolar compartment that is modified by bacterial effectors secreted through the two type iii secretion systems (t3ss-1 and t3ss-2). type iii effectors interact with the host cell endocytic pathway to aid replication. we investigated whether salmonella effector proteins may also interact with the host's exocytic pathway. a se ... | 2013 | 26038467 |
| growth of salmonella enterica and listeria monocytogenes on fresh-cut cantaloupe under different temperature abuse scenarios. | effective cold chain management is a critical component of food safety practice. in this study, we examined the impact of commonly encountered temperature abuse scenarios on the proliferation of salmonella enterica and listeria monocytogenes on fresh-cut cantaloupe. inoculated fresh-cut cantaloupe cubes were subjected to various temperature abuse conditions, and the growth of s. enterica and l. monocytogenes was determined. during 1 week of storage, salmonella cell counts on fresh-cut cantaloupe ... | 2015 | 26038902 |
| improvements to a pcr-based serogrouping scheme for salmonella enterica from dairy farm samples. | molecular serotyping through the use of pcr is a simple and useful technique for characterizing isolates of salmonella enterica subsp. enterica belonging to serogroups b, c1, c2, d1, and e1, which are the majority of the isolates associated with human disease outbreaks. however, many of the salmonella strains currently isolated from dairy farms in the northeastern united states are serovar cerro, a group k strain not detected by this assay. primers from a well-known pcr assay for the identificat ... | 2015 | 26038910 |
| comparative effects of ohmic and conventional heating for inactivation of escherichia coli o157:h7, salmonella enterica serovar typhimurium, and listeria monocytogenes in skim milk and cream. | ohmic heating has proven advantages over conventional thermal processing and novel thermal alternative technologies. in this study, the effect of ohmic and conventional heating for pasteurizing skim milk and cream was examined. all treatment conditions for ohmic and conventional heating were identical except for composition of the heating chamber. in most cases, the reduction of three pathogens did not differ significantly between ohmic heating and conventional heating at fixed treatment tempera ... | 2015 | 26038915 |
| genomic comparison of the closely-related salmonella enterica serovars enteritidis, dublin and gallinarum. | the salmonella enterica serovars enteritidis, dublin, and gallinarum are closely related but differ in virulence and host range. to identify the genetic elements responsible for these differences and to better understand how these serovars are evolving, we sequenced the genomes of enteritidis strain lk5 and dublin strain sarb12 and compared these genomes to the publicly available enteritidis p125109, dublin ct 02021853 and dublin sd3246 genome sequences. we also compared the publicly available g ... | 2015 | 26039056 |
| transcriptional and post-transcriptional modulation of spi1 and spi2 expression by ppgpp, rpos and dksa in salmonella enterica sv typhimurium. | the expression of genes within salmonella pathogenicity islands 1 and 2 (spi1, spi2) is required to facilitate invasion and intracellular replication respectively of s. typhimurium in host cell lines. control of their expression is complex and occurs via a variety of factors operating at transcriptional and post-transcriptional levels in response to the environmental stimuli found within the host. several of the factors that modulate spi1 and spi2 expression are involved in the redistribution or ... | 2015 | 26039089 |
| proanthocyanidins in an astringent persimmon inhibit salmonella pathogenicity island 1 (spi1) secretion. | astringent compounds contained in persimmon fruits have been widely used in japan as food preservatives and thus as anti-bacterial and anti-fungi reagents. however, the molecular mechanism of the anti-microbial activity has been unclear. one of the virulence secretion systems in salmonella enterica was used to test the anti-microbial activity of extracts from a persimmon (diospyros kaki thunb 'saijo'). | 2016 | 26041125 |
| application of bacteriophages in post-harvest control of human pathogenic and food spoiling bacteria. | bacteriophages have attracted great attention for application in food biopreservation. lytic bacteriophages specific for human pathogenic bacteria can be isolated from natural sources such as animal feces or industrial wastes where the target bacteria inhabit. lytic bacteriophages have been tested in different food systems for inactivation of main food-borne pathogens including listeria monocytogenes, staphylococcus aureus, escherichia coli o157:h7, salmonella enterica, shigella spp., campylobac ... | 2016 | 26042353 |
| effect of milk fat content on the performance of ohmic heating for inactivation of escherichia coli o157:h7, salmonella enterica serovar typhimurium and listeria monocytogenes. | the effect of milk fat content on ohmic heating compared to conventional heating for inactivation of food-borne pathogens was investigated. | 2015 | 26043029 |
| low dietary iron intake restrains the intestinal inflammatory response and pathology of enteric infection by food-borne bacterial pathogens. | orally administrated iron is suspected to increase susceptibility to enteric infections among children in infection endemic regions. here we investigated the effect of dietary iron on the pathology and local immune responses in intestinal infection models. mice were held on iron-deficient, normal iron, or high iron diets and after 2 weeks they were orally challenged with the pathogen citrobacter rodentium. microbiome analysis by pyrosequencing revealed profound iron- and infection-induced shifts ... | 2015 | 26046550 |
| antimicrobial susceptibility and oxymino-β-lactam resistance mechanisms in salmonella enterica and escherichia coli isolates from different animal sources. | the impact of extended-spectrum β-lactamases (esbls) and plasmid-mediated ampc β-lactamases (pmaβs) of animal origin has been a public health concern. in this study, 562 salmonella enterica and 598 escherichia coli isolates recovered from different animal species and food products were tested for antimicrobial resistance. detection of esbl-, pmaβ-, plasmid-mediated quinolone resistance (pmqr)-encoding genes and integrons was performed in isolates showing non-wild-type phenotypes. susceptibility ... | 2015 | 26054292 |
| close genetic relationship between salmonella enterica serovar enteritidis isolated from patients with diarrhoea and poultry in the republic of korea. | 2015 | 26055417 | |
| flagellin is required for host cell invasion and normal salmonella pathogenicity island 1 expression by salmonella enterica serovar paratyphi a. | salmonella enterica serovar paratyphi a is a human-specific serovar that, together with salmonella enterica serovar typhi and salmonella enterica serovar sendai, causes enteric fever. unlike the nontyphoidal salmonella enterica serovar typhimurium, the genomes of s. typhi and s. paratyphi a are characterized by inactivation of multiple genes, including in the flagellum-chemotaxis pathway. here, we explored the motility phenotype of s. paratyphi a and the role of flagellin in key virulence-associ ... | 2015 | 26056383 |
| comparison of fresh and frozen fecal samples for detection of enteric salmonella from captive indian star tortoises (geochelone elegans). | the use of frozen fecal samples in enrichment media to detect salmonella spp. strains was evaluated in indian star tortoises (geochelone elegans) to determine the utility of this test method for field collection. fresh fecal samples were collected from 10 captive adult indian star tortoises. each sample was split, with one portion in enrichment media at room temperature for 1 or 2 days before submission to a reference laboratory for standard enteric culture. the other was placed in the same enri ... | 2015 | 26056867 |
| prevalence of salmonella in captive reptiles from croatia. | salmonellosis transmitted by pet reptiles is an increasing public health issue worldwide. the aim of this study was to investigate the prevalence of salmonella strains from captive reptiles in croatia. from november 2009 to november 2011 a total of 292 skin, pharyngeal, cloacal, and fecal samples from 200 apparently healthy reptiles were tested for salmonella excretions by bacteriologic culture and serotyping. these 200 individual reptiles included 31 lizards, 79 chelonians, and 90 snakes belong ... | 2015 | 26056873 |
| 5'-utr of mals increases the invasive capacity of salmonella enterica serovar typhi by influencing the expression of bax. | an rna-seq analysis recently identified a 236-nucleotide transcript upstream from mals in salmonella enterica serovar typhi. here, we investigated its molecular characteristics and function. | 2015 | 26059618 |
| cyclic rhamnosylated elongation factor p establishes antibiotic resistance in pseudomonas aeruginosa. | elongation factor p (ef-p) is a ubiquitous bacterial protein that is required for the synthesis of poly-proline motifs during translation. in escherichia coli and salmonella enterica, the posttranslational β-lysylation of lys34 by the poxa protein is critical for ef-p activity. poxa is absent from many bacterial species such as pseudomonas aeruginosa, prompting a search for alternative ef-p posttranslation modification pathways. structural analyses of p. aeruginosa ef-p revealed the attachment o ... | 2015 | 26060278 |
| small molecules with big effects: cyclic di-gmp-mediated stimulation of cellulose production by the amino acid ʟ-arginine. | in this issue of science signaling, mills et al. show that the amino acid ʟ-arginine increases the concentration of the second messenger c-di-gmp in salmonella enterica serovar typhimurium through a specific diguanylate cyclase, leading to increased production of the exopolysaccharide cellulose, which is an extracellular matrix component of environmental and host-associated biofilms. | 2015 | 26060328 |
| diffusion and persistence of multidrug resistant salmonella typhimurium strains phage type dt120 in southern italy. | sixty-two multidrug resistant salmonella enterica serovar typhimurium strains isolated from 255 clinical strains collected in southern italy in 2006-2008 were characterised for antimicrobial resistance genes, pulsotype, and phage type. most strains (83.9%) were resistant to ampicillin, chloramphenicol, streptomycin, sulfamethoxazole, and tetracycline (acssut) encoded in 88.5% by the salmonella genomic island (sgi1) and in 11.5% by the inh-like integron (bla oxa-30-aada1) and cata1, sul1, and tet ... | 2015 | 26060815 |
| infection with salmonella enterica serovar typhimurium leads to increased proportions of f4/80+ red pulp macrophages and decreased proportions of b and t lymphocytes in the spleen. | infection of mice with salmonella enterica serovar typhimurium (salmonella) causes systemic inflammatory disease and enlargement of the spleen (splenomegaly). splenomegaly has been attributed to a general increase in the numbers of phagocytes, lymphocytes, as well as to the expansion of immature cd71+ter119+ reticulocytes. the spleen is important for recycling senescent red blood cells (rbcs) and for the capture and eradication of blood-borne pathogens. conservation of splenic tissue architectur ... | 2015 | 26068006 |
| optical imaging of paramagnetic bead-dna aggregation inhibition allows for low copy number detection of infectious pathogens. | dna-paramagnetic silica bead aggregation in a rotating magnetic field facilitates the quantification of dna with femtogram sensitivity, but yields no sequence-specific information. here we provide an original description of aggregation inhibition for the detection of dna and rna in a sequence-specific manner following loop-mediated isothermal amplification (lamp). the fragments generated via lamp fail to induce chaotrope-mediated bead aggregation; however, due to their ability to passivate the b ... | 2015 | 26068926 |
| protozoan cysts act as a survival niche and protective shelter for foodborne pathogenic bacteria. | the production of cysts, an integral part of the life cycle of many free-living protozoa, allows these organisms to survive adverse environmental conditions. given the prevalence of free-living protozoa in food-related environments, it is hypothesized that these organisms play an important yet currently underinvestigated role in the epidemiology of foodborne pathogenic bacteria. intracystic bacterial survival is highly relevant, as this would allow bacteria to survive the stringent cleaning and ... | 2015 | 26070667 |
| acetylation regulates survival of salmonella enterica serovar typhimurium under acid stress. | the ability to acetylate lysine residues is conserved across organisms, and acetylation of lysine residues plays important roles in various cellular functions. maintaining intracellular ph homeostasis is crucial for the survival of enteric bacteria in the acidic gastric tract. it has been shown that eukaryotes can stabilize the intracellular ph by histone deacetylation. however, it remains unknown whether bacteria can utilize a reversible protein acetylation system to adapt to an acidic environm ... | 2015 | 26070677 |
| an enterobacterial common antigen mutant of salmonella enterica serovar typhimurium as a vaccine candidate. | due to increasing rates of invasive salmonella enterica serovar typhimurium infection, there is a need for an effective vaccine to prevent this disease. previous studies showed that a mutation in the first gene of the enterobacterial common antigen biosynthetic pathway, weca, resulted in attenuation of s. typhimurium in a murine model of salmonellosis. furthermore, immunization with a weca(-) strain protected against lethal challenge with the parental wild type s. typhimurium strain. herein, we ... | 2015 | 26070977 |
| erratum for parry et al., clinically and microbiologically derived azithromycin susceptibility breakpoints for salmonella enterica serovars typhi and paratyphi a. | 2015 | 26071570 | |
| characterization of salmonella enterica serovar typhimurium and salmonella enterica serovar 4,[5],12:i:- isolates from pigs presenting with diarrhea in korea. | between 2011 and 2012, a total of 896 pig fecal samples were collected from nine provinces in korea, and 50 salmonella enterica susp. enterica serovar typhimurium (s. typhimurium) was isolated. the characteristics of the 50 strains were analyzed, and 4 strains were identified as salmonella enterica subsp. enterica serovar 4,[5],12:i:-. salmonella 4,[5],12:i:- could not be distinguished from s. typhimurium through phage typing, antimicrobial resistance testing or multiple-locus variable-number ta ... | 2015 | 26074410 |
| differential induction of total ige by two salmonella enterica serotypes. | the main goal of this study was to establish how the inflammation caused by infection with two different salmonella enterica serotypes, s. typhimurium and s. enteritidis, may lead to the predisposition to allergy as measured by total ige level in the blood. infection by s. typhimurium did not affect the systemic ige concentration while in s. enteritidis-infected patients there was a significant 3.5-fold increase. this effect was especially profound in patients >4 years old, with up to the 8-fold ... | 2015 | 26075186 |
| fluoroquinolone induction of phage-mediated gene transfer in multidrug-resistant salmonella. | fluoroquinolones are broad-spectrum antibiotics that inhibit bacterial dna gyrase and topoisomerase activity, which can cause dna damage and result in bacterial cell death. in response to dna damage, bacteria induce an sos response to stimulate dna repair. however, the sos response may also induce prophage with production of infectious virions. salmonella strains typically contain multiple prophages, and certain strains including phage types dt120 and dt104 contain prophage that upon induction a ... | 2015 | 26078016 |
| immunomagnetic separation of salmonella with tailored magnetic micro and nanocarriers. a comparative study. | this paper addresses a comparative study of immunomagnetic separation of salmonella using micro and nano-sized magnetic carriers. in this approach, nano (300 nm) and micro (2.8 μm) sized magnetic particles were modified with anti-salmonella antibody to pre-concentrate the bacteria from the samples throughout an immunological reaction. the performance of the immunomagnetic separation on the different magnetic carriers was evaluated using classical culturing, confocal and scanning electron microsc ... | 2015 | 26078149 |
| a macrophage subversion factor is shared by intracellular and extracellular pathogens. | pathogenic bacteria have developed strategies to adapt to host environment and resist host immune response. several intracellular bacterial pathogens, including salmonella enterica and mycobacterium tuberculosis, share the horizontally-acquired mgtc virulence factor that is important for multiplication inside macrophages. mgtc is also found in pathogenic pseudomonas species. here we investigate for the first time the role of mgtc in the virulence of an extracellular pathogen, pseudomonas aerugin ... | 2015 | 26080006 |
| crystal structure of dsba from corynebacterium diphtheriae and its functional implications for cuep in gram-positive bacteria. | in gram-negative bacteria in the periplasmic space, the dimeric thioredoxin-fold protein dsbc isomerizes and reduces incorrect disulfide bonds of unfolded proteins, while the monomeric thioredoxin-fold protein dsba introduces disulfide bonds in folding proteins. in the gram-negative bacteria salmonella enterica serovar typhimurium, the reduced form of cuep scavenges the production of hydroxyl radicals in the copper-mediated fenton reaction, and dsbc is responsible for keeping cuep in the reduced ... | 2015 | 26082031 |
| one health and food-borne disease: salmonella transmission between humans, animals, and plants. | there are >2,600 recognized serovars of salmonella enterica. many of these salmonella serovars have a broad host range and can infect a wide variety of animals, including mammals, birds, reptiles, amphibians, fish, and insects. in addition, salmonella can grow in plants and can survive in protozoa, soil, and water. hence, broad-host-range salmonella can be transmitted via feces from wild animals, farm animals, and pets or by consumption of a wide variety of common foods: poultry, beef, pork, egg ... | 2014 | 26082128 |
| understanding paratyphoid infection: study protocol for the development of a human model of salmonella enterica serovar paratyphi a challenge in healthy adult volunteers. | this study will develop the first human challenge model of paratyphoid infection which may then be taken forward to evaluate paratyphoid vaccine candidates. salmonella paratyphi a is believed to cause a quarter of the estimated 20 million cases of enteric fever annually. epidemiological evidence also suggests that an increasing proportion of the enteric fever burden is attributable to s. paratyphi infection meriting further attention and interest in vaccine development. assessment of paratyphoid ... | 2015 | 26082464 |
| genomic comparison of non-typhoidal salmonella enterica serovars typhimurium, enteritidis, heidelberg, hadar and kentucky isolates from broiler chickens. | non-typhoidal salmonella enterica serovars, associated with different foods including poultry products, are important causes of bacterial gastroenteritis worldwide. the colonization of the chicken gut by s. enterica could result in the contamination of the environment and food chain. the aim of this study was to compare the genomes of 25 s. enterica serovars isolated from broiler chicken farms to assess their intra- and inter-genetic variability, with a focus on virulence and antibiotic resistan ... | 2015 | 26083489 |
| results of salmonella enterica subsp. enterica serotype identification by salmonella check&trace microarray in international external quality assurance systems. | traditionally salmonella enterica subsp. enterica serotypes are identified by slide agglutination with specific antisera for somatic, flagellar and sometimes capsular antigens. an alternative way is genoserotyping using for example a microarray, eg. commercially available test check&trace salmonella. the goal of this study was to evaluate the check&trace salmonella microarray for salmonella enterica subsp. enterica serotype identification, using salmonella strains provided by reference laborator ... | 2015 | 26084071 |
| construction of an attenuated salmonella enterica serovar paratyphi a vaccine strain harboring defined mutations in htra and yncd. | the global epidemic features of enteric fever have changed greatly in recent years. the incidence of enteric fever caused by salmonella enterica serovar paratyphi a has progressively increased. in some areas of asia, infections with s. paratyphi a have exceeded those with s. typhi, resulting in s. paratyphi a becoming the main causative agent of enteric fever. however, two currently licensed typhoid vaccines do not confer adequate cross-protection against s. paratyphi a infection. therefore, dev ... | 2015 | 26084199 |
| occurrence and antimicrobial resistance of salmonella strains from food of animal origin in southern italy. | six hundred fourteen strains of salmonella enterica were isolated from 16,926 samples of food of animal origin collected in southern italy from 2003 to 2012. the isolates were identified, serotyped, and challenged against 15 antibiotics according to the protocol defined at national level for veterinary isolates of salmonella (entervet surveillance network). salmonella serotypes typhimurium, hadar, enteritidis, derby, and 4,[5],12:i:- were those most frequently isolated. the widest resistances we ... | 2016 | 26084745 |
| the anti-infective activity of punicalagin against salmonella enterica subsp. enterica serovar typhimurium in mice. | punicalagin, a major bioactive component of pomegranate peel, has been proven to have antioxidant, antiviral, anti-apoptosis, and hepatoprotective properties. the aim of this study was to investigate the anti-infective activity of punicalagin in a mouse model. c57bl/6 mice were initially challenged with salmonella enterica subsp. enterica serovar typhimurium (s. typhimurium) and then treated with punicalagin. food and water consumption and body weight were recorded daily. on day 8 post infection ... | 2015 | 26084785 |
| ovariectomy as treatment for ovarian bacterial granulomas in a duvaucel's gecko (hoplodactylus duvaucelii). | an adult female duvaucel's gecko (hoplodactylus duvaucelii) from a threatened species breeding programme presented due to a prolonged gestation period and distended abdomen. | 2015 | 26085121 |
| fast-sl: an efficient algorithm to identify synthetic lethal sets in metabolic networks. | synthetic lethal sets are sets of reactions/genes where only the simultaneous removal of all reactions/genes in the set abolishes growth of an organism. previous approaches to identify synthetic lethal genes in genome-scale metabolic networks have built on the framework of flux balance analysis (fba), extending it either to exhaustively analyze all possible combinations of genes or formulate the problem as a bi-level mixed integer linear programming (milp) problem. we here propose an algorithm, ... | 2015 | 26085504 |
| first report of anaerobic isolation of salmonella enterica from liver abscesses of feedlot cattle. | 2015 | 26085617 | |
| complete genome sequencing of a multidrug-resistant and human-invasive salmonella enterica serovar typhimurium strain of the emerging sequence type 213 genotype. | salmonella enterica subsp. enterica serovar typhimurium strain yu39 was isolated in 2005 in the state of yucatán, mexico, from a human systemic infection. the yu39 strain is representative of the multidrug-resistant emergent sequence type 213 (st213) genotype. the yu39 complete genome is composed of a chromosome and seven plasmids. | 2015 | 26089426 |
| characterization of the invasive, multidrug resistant non-typhoidal salmonella strain d23580 in a murine model of infection. | a distinct pathovar of salmonella enterica serovar typhimurium, st313, has emerged in sub-saharan africa as a major cause of fatal bacteremia in young children and hiv-infected adults. d23580, a multidrug resistant clinical isolate of st313, was previously shown to have undergone genome reduction in a manner that resembles that of the more human-restricted pathogen, salmonella enterica serovar typhi. it has since been shown through tissue distribution studies that d23580 is able to establish an ... | 2015 | 26091096 |