Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| cloning and comparison of phylogenetically related chitinases from listeria monocytogenes egd and enterococcus faecalis v583. | to compare enzymatic activities of two related chitinases, chia and ef0361, encoded by listeria monocytogenes and enterococcus faecalis, respectively. | 2009 | 19583793 |
| leakage of teeth root-filled with guttaflow and a single gp cone compared to lateral condensation and warm vertical condensation. | root canal obturation can be time consuming and complicated. guttaflow is suggested for use with a simple, single cone obturating technique. | 2009 | 19585911 |
| hygienic effects and gas production of plastic bio-digesters under tropical conditions. | plastic plug-flow bio-digesters have been promoted as a good option for improved treatment of manure and wastewater in developing countries although minimal information has been published on their hygienic status. this bench-scale study replicates bio-digester conditions to evaluate the reduction of pathogen and indicator microorganisms at three different hydraulic retention times (hrt) in the anaerobic treatment of pig manures at 30 degrees c for 50 days. results showed that physicochemical val ... | 2009 | 19590126 |
| rapid dead-end ultrafiltration concentration and biosensor detection of enterococci from beach waters of southern california. | same-day microbial water quality assessments are not possible with standard methods, which increases the possibility of public exposure to fecal pathogens. this study examined the efficacy of high-volume hollow fibre ultrafiltration coupled to biosensor detection for enterococci in marine waters to allow same-day public notification of poor water quality. fifty-six 1001 ultrafiltered samples and 100 ml grab samples were collected weekly from may to july 2007. post-ultrafiltration processing incl ... | 2009 | 19590135 |
| mathematical models for enterococcus faecalis recovery after microwave water disinfection. | microwave water disinfection is a rapid purification technique which can give billions of people access to clean drinking water. however, better understanding of bacterial recovery after microwave heating over time is necessary to determine parameters such as delayed bacterial growth rates and maximum bacterial yields. mathematical models for enterococcus faecalis recovery after microwave treatment in optimum growth conditions were developed for times up to 5 minutes using an optical absorbance ... | 2009 | 19590138 |
| characterization of salivaricin crl 1328, a two-peptide bacteriocin produced by lactobacillus salivarius crl 1328 isolated from the human vagina. | salivaricin crl 1328 is a heat-stable bacteriocin produced by lactobacillus salivarius crl 1328, a strain isolated from healthy human vagina, with potential applications for preventing urogenital infections. the objective of this study was to characterize the locus responsible for salivaricin crl 1328 production and its mechanism of action against enterococcus faecalis mp97 as the sensitive strain. oligonucleotides were designed based on sequences of antimicrobial peptides previously described i ... | 2009 | 19591924 |
| [a clinical study of bile cultures and antibiotic susceptibility test in the patients with operation on biliary tract]. | to illustrate the bacteriology and their susceptibility to antibiotics in patients with biliary tract diseases and provide information for antibiotic choices. | 2009 | 19595212 |
| validation and field testing of library-independent microbial source tracking methods in the gulf of mexico. | water quality is frequently impacted by microbial pollution from human and animal feces. microbial source tracking (mst) can identify dominant pollution sources and improve assessment of health risk compared to indicator bacteria alone. this study aims to standardize and validate mst methods across laboratories in coastal gulf of mexico states. three laboratories evaluated library-independent mst methods for human sewage detection via conventional pcr: (1) human-associated bacteroidales, (2) hum ... | 2009 | 19595426 |
| direct stimulus perception and transcription activation by a membrane-bound dna binding protein. | few membrane proteins with a role in transcriptional regulation have been studied, and none are able to perceive their respective stimuli and activate transcription of their regulons without the aid of auxiliary proteins. the bacitracin resistance regulator, bcrr, of enterococcus faecalis is a membrane-bound dna binding protein and is required for bacitracin-dependent expression of the bacitracin resistance genes, bcrabd. here, we show that bcrr interacts directly with zn2+ bacitracin (kd = 2-5 ... | 2009 | 19602149 |
| [enterococcal meningitis in adults]. | in recent years, enterococcus species have emerged as significant human pathogens capable of causing a variety of nosocomial and community-acquired infections. however, enterococcal meningitis (em) is an uncommon disease. this study describes the clinical features and outcome of em in adults seen at a tertiary hospital during 25 years. | 2009 | 19603294 |
| molecular identification of coliform bacteria from colicky breastfed infants. | to determine the presence of intestinal coliform bacteria in colicky vs healthy infants. | 2009 | 19604166 |
| protein c concentrate in preterm neonates with sepsis. | 2009 | 19604168 | |
| characterization of clinical enterococcus faecalis small-colony variants. | in this report, we present a clinical case of chronic aortic valve endocarditis caused by enterococcus faecalis small-colony variants (scvs), with ensuing characterization of the scv phenotype in comparison to the clonally related normal phenotype with respect to alterations in microscopic and ultrastructural morphology, growth behavior, and metabolic pathways. in contrast to the normal phenotype, light and electron microscopy of the enterococcus scvs demonstrated the presence of heterogeneous c ... | 2009 | 19605585 |
| the (p)ppgpp synthetase rela contributes to stress adaptation and virulence in enterococcus faecalis v583. | guanosine penta- and tetraphosphate [(p)ppgpp] are two unusual nucleotides implied in the bacterial stringent response. in many pathogenic bacteria, mutants unable to synthesize these molecules lose their virulence. in gram-positive bacteria such as enterococcus faecalis, the synthesis and degradation of (p)ppgpp mainly depend on the activity of a bifunctional enzyme, encoded by the rela gene. by analysing deltarela and deltarelq (which encodes a protein harbouring a ppgpp synthetase activity) d ... | 2009 | 19608607 |
| in vitro antimicrobial activity of 0.5%, 1%, and 2.5% sodium hypochlorite in root canals instrumented with the protaper universal system. | the purpose of this in vitro study was to evaluate the antimicrobial activity of 0.5%, 1% and 2.5% sodium hypochlorite in root canals instrumented with the protaper universal system. | 2009 | 19615646 |
| shelf life, dissolving action, and antibacterial activity of a neutralized 2.5% sodium hypochlorite solution. | the aim was to evaluate the shelf life and the dissolving and antibacterial properties of a neutralized 2.5% naocl solution. | 2009 | 19615648 |
| in vitro antimicrobial activity of calcium hydroxide mixed with different vehicles against enterococcus faecalis and candida albicans. | the purpose of this in vitro study was to investigate antimicrobial activity of calcium hydroxide (ch) in combination with glycerin, chlorhexidine gluconate (chx), cetrimide, or distilled water against enterococcus faecalis and candida albicans. | 2009 | 19615665 |
| synthesis, characterization and evaluation of antibacterial activity of some thiazolo[3,2-b][1,2,4]triazole incorporating diphenylsulfone moieties. | a series of thiazolo[3,2-b][1,2,4]triazole incorporating diphenylsulfone moieties were synthesized starting from 5-[4-(4-x-phenylsulfonyl)phenyl]-4h-1,2,4-triazole-3-thioles 3a-c, x=h, cl, br. thus, alkylation of 1,2,4-triazoles 3 with phenacyl bromide or 4-bromophenacyl bromide afforded s-substituted 1,2,4-triazoles 4, 5. these new intermediates 4 and 5, in the presence of h(2)so(4) (c), were cyclized to 2-[4-(4-x-phenylsulfonyl)phenyl]-6-(4-y-phenyl)[1,3]thiazolo[3,2-b]-[1,2,4]-triazoles 6, 7 ... | 2009 | 19616347 |
| enterococcus faecalis translocation in mice with severe burn injury: a pathogenic role of ccl2 and alternatively activated macrophages (m2amphi and m2cmphi). | here, we investigated a role of ccl2 on the increased susceptibility of severely burned mice to enterococcus faecalis translocation. after inoculation of mphi from mlmphi of normal mice, 80% of the scidbgmn mice orally infected with the lethal dose of e. faecalis survived, and all mice inoculated with mlmphi from thermally injured mice died. at this time, scidbgmn mice inoculated with mlmphi from thermally injured ccl2(-/-) mice were shown to be resistant (90% survival). m1mphi were not induced ... | 2009 | 19622799 |
| [vancomycin-resistant enterococci: prevalence and factors associated with intestinal colonization in oncology patients from hospital de niños de córdoba]. | vancomycin-resistant enterococci (vre) have an important impact on pediatric oncology population. the objectives of this study were: to know the prevalence of vre intestinal colonization in oncology patients, to identify the risk factors that predispose hospitalized patients to vre intestinal colonization, and to determine the vre resistance profile to different antimicrobial agents. we studied all children with oncological disease aged 1 month to 16 years that had joined the protocol and had be ... | 2009 | 19623898 |
| uropathogen interaction with the surface of urological stents using different surface properties. | ureteral stents commonly become infected or encrusted. various coatings have been developed to decrease bacterial adherence. to our knowledge there has been no in vitro testing of coating with heparin to date. we determined the effects of heparin coating on bacterial adherence of common uropathogens and physical stent properties. | 2009 | 19625060 |
| phytochemical, antibacterial, antiproliferative, and antioxidant potentials and dna damage-protecting activity of acacia salicina extracts. | the extract enriched in total oligomer flavonoids (tof), and the aqueous, methanol, and ethyl acetate extracts of acacia salicina were investigated for their polyphenolic compound content, antioxidative activity in the nitro blue tetrazolium/riboflavin assay system, antibacterial activity against gram-positive and gram-negative bacterial reference strains, antigenotoxic activity tested with the ames assay, and cytotoxic activity against the k562 human chronic myelogenous leukemia cell line and l ... | 2009 | 19627220 |
| population structure and safety aspects of enterococcus strains isolated from artisanal dry fermented sausages produced in argentina. | enterococci population from argentinean artisanal dry fermented sausage was identified and their safety aspects were evaluated. species-specific pcr was used to distinguish between enterococcus faecium (56%) and enterococcus faecalis (17%). other isolates (27%) were identified as enterococcus durans, enterococcus casseliflavus and enterococcus mundtii by using 16s rna gene sequence. rapd analyses showed different biotypes for ent. faecium and ent. faecalis species. low incidence of antibiotic re ... | 2009 | 19627479 |
| rapid qpcr-based assay for fecal bacteroides spp. as a tool for assessing fecal contamination in recreational waters. | concentrations of fecal indicator bacteria (fib; e.g. escherichia coli, and enterococcus sp.) can only be used in limited ways for determining the source of fecal contamination in recreational waters because they cannot distinguish human from non-human fecal contamination. several bacteroides spp. have been suggested as potential alternative indicators. we have developed a rapid, culture-independent method for quantifying fecal bacteroides spp. using quantitative pcr (qpcr) targeting the 16s rrn ... | 2009 | 19631958 |
| evaluation of dipstick analysis among elderly residents to detect bacteriuria: a cross-sectional study in 32 nursing homes. | few studies have evaluated dipstick urinalysis for elderly and practically none present confidence intervals. furthermore, most previous studies combine all bacteria species in a "positive culture". thus, their evaluation may be inappropriate due to yule-simpson's paradox. the aim of this study was to evaluate diagnostic accuracy of dipstick urinalysis for the elderly in nursing homes. | 2009 | 19635163 |
| in vivo antibacterial activity of vertilmicin, a new aminoglycoside antibiotic. | vertilmicin is a novel aminoglycoside antibiotic with potent activity against gram-negative and -positive bacteria in vitro. in this study, we further evaluated the efficacy of vertilmicin in vivo in systemic and local infection animal models. we demonstrated that vertilmicin had relatively high and broad-spectrum activities against mouse systemic infections caused by escherichia coli, klebsiella pneumoniae, staphylococcus aureus, and enterococcus faecalis. the 50% effective doses of subcutaneou ... | 2009 | 19635958 |
| detection of helicobacter pylori, enterococcus faecalis, and pseudomonas aeruginosa in the subgingival biofilm of hiv-infected subjects undergoing haart with chronic periodontitis. | this study compared the frequency of helicobacter pylori, enterococcus faecalis, and pseudomonas aeruginosa in the subgingival microbiota of hiv-seropositive and hiv-seronegative subjects with periodontitis or clinically healthy periodontal tissues. fifty-four subjects were distributed into two hiv-seropositive groups (chronic periodontitis [hcp = 13] and periodontal health [hh = 10]) and two hiv-seronegative groups (chronic periodontitis [cp = 17] and periodontal health [h = 14]). the detection ... | 2009 | 19639349 |
| characterisation of a small erythromycin resistance plasmid plfe1 from the food-isolate lactobacillus plantarum m345. | this paper reports the complete 4031 bp nucleotide sequence of the small erythromycin resistance plasmid plfe1 isolated from the raw-milk cheese isolate lactobacillus plantarum m345. analysis of the sequence revealed the coding regions for the erythromycin resistance determinant erm(b). a replication initiation protein repb was identified belonging to the repb proteins of the pmv158 family of rolling-circle replicating plasmids. the transcriptional repressor protein copg and a small counter tran ... | 2009 | 19640422 |
| innate and adaptive immunity cooperate flexibly to maintain host-microbiota mutualism. | commensal bacteria in the lower intestine of mammals are 10 times as numerous as the body's cells. we investigated the relative importance of different immune mechanisms in limiting the spread of the intestinal microbiota. here, we reveal a flexible continuum between innate and adaptive immune function in containing commensal microbes. mice deficient in critical innate immune functions such as toll-like receptor signaling or oxidative burst production spontaneously produce high-titer serum antib ... | 2009 | 19644121 |
| antibacterial compounds from mushrooms i: a lanostane-type triterpene and prenylphenol derivatives from jahnoporus hirtus and albatrellus flettii and their activities against bacillus cereus and enterococcus faecalis. | antibacterial bioassay-guided fractionation of two american mushroom species, jahnoporus hirtus and albatrellus flettii, led to the isolation and identification of their major antibacterial constituents: 3,11-dioxolanosta-8,24( z)-diene-26-oic acid (1) from j. hirtus and confluentin (2), grifolin (3), and neogrifolin (4) from a. flettii. compound 1 is a new lanostane-type triterpene. all purified compounds were evaluated for their ability to inhibit the growth of bacillus cereus and enterococcus ... | 2010 | 19644795 |
| survival of listeria monocytogenes, listeria innocua, and lactic acid bacteria in chill brines. | listeria monocytogenes is the pathogen of concern in ready-to-eat (rte) meat products. salt brines are used to chill processed meats. l. monocytogenes and lactic acid bacteria (lab) can grow under saline conditions, and may compete with each other for nutrients. the objective of this study was to determine the effect of lactic acid bacteria (enterococcus faecalis, carnobacterium gallinarum, and lactobacillus plantarum) on the survival of l. monocytogenes and listeria innocua in brines stored und ... | 2009 | 19646051 |
| aryl rhodanines specifically inhibit staphylococcal and enterococcal biofilm formation. | staphylococcus epidermidis and staphylococcus aureus are the leading causative agents of indwelling medical device infections because of their ability to form biofilms on artificial surfaces. here we describe the antibiofilm activity of a class of small molecules, the aryl rhodanines, which specifically inhibit biofilm formation of s. aureus, s. epidermidis, enterococcus faecalis, e. faecium, and e. gallinarum but not the gram-negative species pseudomonas aeruginosa or escherichia coli. the aryl ... | 2009 | 19651903 |
| trends in udder health and emerging mastitogenic pathogens in south african dairy herds. | the aim of this study was to retrospectively analyse the results of milk samples obtained from south african dairy herds during the period 1996 to april 2007 in order to identify possible trends in isolates of microorganisms and their pathogenicity under field conditions. milk samples were obtained from 7 of the 9 provinces in south africa where there are low numbers of dairy cows. although there is scientific limitation to a country wide survey, such as the variation in herd size, management sk ... | 2009 | 19653514 |
| evidence-based prescription of antibiotics in urology: a 5-year review of microbiology. | to analyse the results of positive urine cultures over a 5-year period in a large hospital and urology department (amongst both inpatients and outpatients), assess the prevalence of different organisms and the resistance profiles of a range of antibiotics, and thus provide information on which organisms are likely to cause urosepsis. | 2009 | 19659464 |
| bacterial attachment to iron-impregnated granular activated carbon. | bacterial attachment to iron-impregnated granular activated carbon (fe-gac) was investigated in this study using enterococcus faecalis atcc 10100 and charcoal-based gac. two sets of column experiments were performed under different ionic strengths and ph conditions. breakthrough curves of bacteria were obtained by monitoring effluent. mass recoveries and attachment rate coefficients were quantified from these curves. in addition, characteristics of fe-gac were analyzed using field emission scann ... | 2009 | 19660921 |
| [investigation of enterococcus faecalis antimicrobial resistance]. | we performed an antibiotic resistance study on enterococcus faecalis isolated from intrahospitalary and extrahospitalary samples between january 2004 and january 2008. three different samples were studied; urine, blood and wound swabs, considering a strain per patient. we included in the study a global amount of 3,641 enterococcus faecalis isolations from clinical samples received at hospital universitario reina sofía microbiology service in córdoba (spain). we employed semiautomatic system wide ... | 2009 | 19662543 |
| research of urinary tract infections in family medicine physicians' offices--empiric antimicrobial therapy of urinary tract infections--croatian experience. | in the period between october 1st and november 30th, 2006, we investigated a total of 3188 episodes of uti (802 among males; 2386 among females) recorded in 108 family medicine offices in 20 cities in croatia. the most common utis in women were acute uncomplicated cystitis (62%), complicated utis - cystitis and pyelonephritis (14%), urethritis (9%), acute uncomplicated pyelonephritis (6%), recurrent cystitis (5%), asymptomatic bacteriuria (3%) and recurrent pyelonephritis. the most common utis i ... | 2009 | 19662789 |
| evidence for significantly enhancing reduction of azo dyes in escherichia coli by expressed cytoplasmic azoreductase (azoa) of enterococcus faecalis. | although cytoplasmic azoreductases have been purified and characterized from various bacteria, little evidence demonstrating that these azoreductases are directly involved in azo dye reduction in vivo is known. in order to evaluate the contribution of the enzyme to azo dye reduction in vivo, experiments were conducted to determine the effect of a recombinant cytoplasmic azoreductase (azoa) from enterococcus faecalis expressed in escherichia coli on the rate of metabolism of methyl red, ponceau b ... | 2010 | 19663804 |
| crystal structures of bacterial fabh suggest a molecular basis for the substrate specificity of the enzyme. | fabh (beta-ketoacyl-acyl carrier protein synthase iii) is unique in that it initiates fatty acid biosynthesis, is inhibited by long-chain fatty acids providing means for feedback control of the process, and dictates the fatty acid profile of the organism by virtue of its substrate specificity. we report the crystal structures of bacterial fabh enzymes from four different pathogenic species: enterococcus faecalis, haemophilus influenzae, staphylococcus aureus and escherichia coli. structural data ... | 2009 | 19665020 |
| how common is mrsa in adult septic arthritis? | we determine the proportion of methicillin-resistant staphylococcus aureus (mrsa) in adult septic arthritis patients presenting to the emergency department (ed). | 2009 | 19665261 |
| intra- and interspecies conjugal transfer of tn916-like elements from lactococcus lactis in vitro and in vivo. | tetracycline-resistant lactococcus lactis strains originally isolated from polish raw milk were analyzed for the ability to transfer their antibiotic resistance genes in vitro, using filter mating experiments, and in vivo, using germfree rats. four of six analyzed l. lactis isolates were able to transfer tetracycline resistance determinants in vitro to l. lactis bu2-60, at frequencies ranging from 10(-5) to 10(-7) transconjugants per recipient. three of these four strains could also transfer res ... | 2009 | 19666731 |
| pilot study of ampicillin-ceftriaxone combination for treatment of orthopedic infections due to enterococcus faecalis. | serious enterococcus faecalis infections usually require combination therapy to achieve a bactericidal effect. in orthopedic infections, the prognosis of enterococcal etiology is considered poor, and the use of aminoglycosides is questioned. the ampicillin-ceftriaxone combination has recently been accepted as alternative therapy for enterococcal endocarditis. after one of our patients with endocarditis and vertebral osteomyelitis was cured with ampicillin-ceftriaxone, we started a pilot study of ... | 2009 | 19667290 |
| polyphasic taxonomic studies of lactic acid bacteria associated with tunisian fermented meat based on the heterogeneity of the 16s-23s rrna gene intergenic spacer region. | the objective of this work was to investigate the structure and diversity of lactic acid bacteria (lab) communities in traditionally fermented meat collected from different areas of tunisia. a polyphasic study, which involves phenotypic tests and ribosomal dna-based techniques, was used to identify gram-positive and catalase-negative isolates. pcr amplification of the 16s-23s rdna isr of 102 isolates and other reference lab strains gave (1) one type of rrn operon (m-isr) for lactococci, (2) two ... | 2009 | 19669730 |
| molecular epidemiology of vancomycin-resistant enterococci in singapore. | to characterise the mechanism of glycopeptide resistance, genetic relatedness, and pathogenicity factors in isolates of vancomycin-resistant enterococci (vre) in singapore. | 2009 | 19670074 |
| antibacterial activity of ethanol extracts of seaweeds against fish bacterial pathogens. | crude extracts were prepared using the solvent ethanol from the seaweeds viz., gracilaria edulis, calorpha peltada and hydroclothres sp. and screening for their antibacterial activity against six bacterial pathogens were also carried out. the test bacterial strains were escherichia coli, enterobacter aerogenes, staphylococcus aureus, pseudomonas aeruginosa, streptococcus faecalis and bacillus cereus. ethanol extract of gracilaria edulis inhibited growth of all the test organisms except bacillus ... | 2009 | 19673167 |
| [antimicrobial activity of tebipenem against various clinical isolates from various specimen, mainly urinary tract]. | tebipenem is the active metabolite of me1211, tebipenem pivoxil, a novel oral carbapenem that possesses potent activity against almost pathogens except for pseudomonas aeruginosa. in this study, we compared the susceptibility of tebipenem with current antibiotics against various organisms isolated from various specimen, mainly urinary tract. tebipenem had a potent activity against neisseria gonorrhoeae; its activity was comparable to it of cefixime that has most potent activity among oral antibi ... | 2009 | 19673353 |
| structure of the enterococcus faecalis eiia(gnt) pts component. | in eubacteria, the utilization of a number of extracellular carbohydrates is mediated by sugar specific phosphoenolepyruvate (pep) dependent sugar phosphotransferase systems (ptss), which simultaneously import und phosphorylate their target sugars. here, we report the crystal structure of the eiia(gnt) component of the so far little investigated enterococcus faecalis gluconate specific pts. the crystal structure shows a tightly interacting dimer of eiia(gnt) which is structurally similar to the ... | 2009 | 19682976 |
| kinetic characterization of human phosphopantothenoylcysteine synthetase. | phosphopantothenoylcysteine synthetase (ppcs) catalyzes the formation of phosphopantothenoylcysteine from (r)-phosphopantothenate and l-cysteine with the concomitant consumption of a nucleotide triphosphate. herein, the human coab gene encoding ppcs is cloned into pet23a and overexpressed in e. coli bl21(de3), to yield 10mg of purified enzyme per liter of culture. detailed kinetic studies found that this ppcs follows a similar bi uni uni bi ping pong mechanism as previously described for the e. ... | 2009 | 19683078 |
| detection of colon flora in peritoneal drain fluid after colorectal surgery: can rt-pcr play a role in diagnosing anastomotic leakage? | a semi-quantitative real-time pcr strategy was developed to identify potential indicator organisms for anastomotic leakage in peritoneal drainage fluid, escherichia coli and enterococcus faecalis. the analytical performance of the amplification method was validated with 10 culture-positive and 7 culture-negative peritoneal drain fluid samples, obtained from 9 different patients with a colorectal anastomosis. real-time pcr results were fully concordant with the microbiological culture results. ho ... | 2009 | 19683548 |
| frequency and characterisation of spontaneous lipiarmycin-resistant enterococcus faecalis mutants selected in vitro. | 2009 | 19683897 | |
| capsular polysaccharide production in enterococcus faecalis and contribution of cpsf to capsule serospecificity. | many bacterial species produce capsular polysaccharides that contribute to pathogenesis through evasion of the host innate immune system. the gram-positive pathogen enterococcus faecalis was previously reported to produce one of four capsule serotypes (a, b, c, or d). previous studies describing the four capsule serotypes of e. faecalis were based on immunodetection methods; however, the underlying genetics of capsule production did not fully support these findings. previously, it was shown that ... | 2009 | 19684130 |
| ph modulation of efflux pump activity of multi-drug resistant escherichia coli: protection during its passage and eventual colonization of the colon. | resistance nodulation division (rnd) efflux pumps of escherichia coli extrude antibiotics and toxic substances before they reach their intended targets. whereas these pumps obtain their energy directly from the proton motive force (pmf), atp-binding cassette (abc) transporters, which can also extrude antibiotics, obtain energy from the hydrolysis of atp. because e. coli must pass through two ph distinct environments of the gastrointestinal system of the host, it must be able to extrude toxic age ... | 2009 | 19684858 |
| impact of peptidoglycan o-acetylation on autolytic activities of the enterococcus faecalis n-acetylglucosaminidase atla and n-acetylmuramidase atlb. | autolysins are potentially lethal enzymes that partially hydrolyze peptidoglycan for incorporation of new precursors and septum cleavage after cell division. here, we explored the impact of peptidoglycan o-acetylation on the enzymatic activities of enterococcus faecalis major autolysins, the n-acetylglucosaminidase atla and the n-acetylmuramidase atlb. we constructed isogenic strains with various o-acetylation levels and used them as substrates to assay e. faecalis autolysin activities. peptidog ... | 2009 | 19686739 |
| ce-duox1/bli-3 generates reactive oxygen species as a protective innate immune mechanism in caenorhabditis elegans. | caenorhabditis elegans was recently developed as a model system to study both pathogen virulence mechanisms and host defense responses. we previously demonstrated that c. elegans produces reactive oxygen species (ros) in response to exposure to the important gram-positive nosocomial pathogen enterococcus faecalis. we also presented evidence of oxidative stress and upregulation of stress responses after exposure to the pathogen. as in mammalian systems, this new work shows that production of ros ... | 2009 | 19687201 |
| vana in enterococcus faecium, enterococcus faecalis, and enterococcus casseliflavus detected in french cattle. | the goal of this study was to assess the presence of enterococci species presenting van-mediated glycopeptide resistance in french cattle. fecal samples were collected from healthy and sick animals, and enterococci were screened for vancomycin resistance. vancomycin resistance was principally encountered in enterococcus gallinarum and enterococcus casseliflavus strains. however, glycopeptide resistance was detected in three different species of enterococci (e. faecalis, e. faecium, and e. cassel ... | 2009 | 19694552 |
| virulence of enterococcus faecalis dairy strains in an insect model: the role of fsrb and gele. | despite the existence of various virulence factors in the enterococcus genus, enterococcal virulence is still a debated issue. a main consideration is the detection of the same virulence genes in strains isolated from nosocomial or community-acquired infections, and from food products. the goal of this study was to evaluate the roles of two well-characterized enterococcal virulence factors, fsr and gelatinase, in the potential virulence of enterococcus faecalis food strains. virulence of unrelat ... | 2009 | 19696101 |
| comparative study of anthocyanin composition, antimicrobial and antioxidant activity in bilberry (vaccinium myrtillus l.) and blueberry (vaccinium corymbosum l.) fruits. | simultaneous comparison of bilberry (vaccinium myrtillus l.) and blueberry (vaccinium corymbosum l) fruits for their anthocyanin composition, antimicrobial and antioxidant activity is reported. the aim of this study was to investigate and to compare anthocyanin composition, antimicrobial and antioxidant activity in bilberry and blueberry fruits and their skins. the investigations revealed that the highest amount of total anthocyanins was observed in fruits skins of blueberry cultivars. the resul ... | 2009 | 19702172 |
| synthesis and antibacterial activity of a series of alpha-substituted acetylpiperazinyl oxazolidinones. | a series of oxazolidinone derivatives with alpha-substituted acetylpiperazinyl groups were prepared. their in vitro antibacterial activities were studied against gram-positive pathogens, including methicillin-resistant staphylococcus aureus, staphylococcus aureus, staphylococcus epidermidis and enterococcus faecalis. the compounds with chloroacetyl-piperazinyl or dichloroacetyl-piperazinyl group were found to have superior antibacterial activities to linezolid against most of tested gram-positiv ... | 2009 | 19703029 |
| effectiveness of propolis and calcium hydroxide as a short-term intracanal medicament against enterococcus faecalis: a laboratory study. | the aim of this study was to investigate the antimicrobial activity of propolis-based intracanal medicament against enterococcus faecalis using infected dentine models, and to compare its antimicrobial efficacy with that of the non-setting calcium hydroxide paste when used as a short-term medication for 1 and 2 days. a total of 50 dentine discs of 7-mm length was obtained from extracted human teeth. five dentine discs were kept sterile to serve as a negative control. the remaining 45 were contam ... | 2009 | 19703075 |
| activity of the enterococcus faecalis eiia(gnt) pts component and its strong interaction with eiib(gnt). | eubacteria can import and simultaneously phosphorylate a range of different carbohydrates by means of sugar specific phosphoenolpyruvate (pep) dependent sugar phosphotransferase systems (ptss). here, we report the biochemical characterization of the gluconate specific pts component eiia(gnt) from enterococcus faecalis and its unexpectedly strong complex with eiib(gnt). we analyze the activity of the complex regarding phosphoryl transfer using kinetic measurements and demonstrate by mutagenesis t ... | 2009 | 19703414 |
| effect of mixed antimicrobial agents and flavors in active packaging films. | active packaging is an emerging food technology to improve the quality and safety of food products. many works have been developed to study the antimicrobial activity of essential oils. essential oils have been traditionally used as flavorings in food, so they have an important odor impact but they have as well antimicrobial properties that could be used to protect the food. recent developments in antimicrobial active packaging showed the efficiency of essential oils versus bread and bakery prod ... | 2009 | 19711918 |
| genome of a virulent bacteriophage lb338-1 that lyses the probiotic lactobacillus paracasei cheese strain. | there is a lack of fundamental knowledge about the influence of bacteriophage on probiotic bacteria and other commensals in the gut. here, we present the isolation and morphological and genetic characterization of a virulent narrow-host-range bacteriophage, philb338-1. this phage was isolated from fresh sewage and was shown to infect the probiotic cheese strain lactobacillus paracasei nfbc 338. electron microscopy studies revealed that philb338-1 is a member of the myoviridae family, with an iso ... | 2009 | 19712732 |
| transepithelial activation of human leukocytes by probiotics and commensal bacteria: role of enterobacteriaceae-type endotoxin. | the goal of the current study was to clarify whether commercially available probiotics induce greater trans-epithelial activation of human leukocytes than do commensal, food-derived and pathogenic bacteria and to identify the compounds responsible for this activation. eleven different bacterial strains, and some of their pathogen-associated molecular patterns, were incubated apically on a confluent layer of intestinal epithelial cells (caco-2), which were basolaterally co-cultured with human mon ... | 2009 | 19714861 |
| comparison of the sealing ability of three filling techniques in canals shaped with two different rotary systems: a bacterial leakage study. | this study compared the sealing ability of 3 current filling techniques in root canals shaped with 2 different rotary systems. | 2009 | 19716483 |
| nosocomial outbreak of linezolid-resistant enterococcus faecalis infection in a tertiary care hospital. | we describe 12 cases of linezolid-resistant enterococcus faecalis. the present study was done in 2 wards of hospital universitario la paz in madrid, spain. the 2 wards involved were the intensive care unit (icu) and reanimation unit. twelve clinical strains of e. faecalis reported by the clinical laboratory as linezolid resistant based on mics determined by e-test (ab biodisk, solna, sweden) were collected between september 2005 and october 2006. the mic of linezolid for all the resistant isolat ... | 2009 | 19717261 |
| the cellulolytic microflora of the human colon: evidence of microcrystalline cellulose-degrading bacteria in methane-excreting subjects. | abstract in humans, plant cell wall polysaccharides (mainly cellulose and hemicelluloses) represent an important source of dietary fibres that are digested by the gut microflora. however, the fibrolytic micro-organisms involved in the breakdown of these substrates remain largely unknown. our objective was to quantify the microcrystalline-cellulose-degrading and methanogenic microbial communities in faecal samples (n=34) from both methane- and non-methane-excreting individuals and to identify the ... | 2003 | 19719585 |
| contemporary root canal irrigants are able to disrupt and eradicate single- and dual-species biofilms. | clinical/microbiological studies have consistently revealed the persistence of some bacteria after conventional root canal debridement. although this was originally attributed to the complexity of the root canal anatomy and the difficulty of delivering antibacterial agents effectively, it has emerged that the biofilm encasement of bacterial cells may confer a further mechanism of resistance. the purpose of this study was to investigate the relative disruption and bactericidal effects of root can ... | 2009 | 19720223 |
| influence of streptococcus mutans on enterococcus faecalis biofilm formation. | an important virulence factor of enterococcus faecalis is its ability to form biofilms. most studies on biofilm formation have been carried out by using e. faecalis monocultures. given the polymicrobial nature of root canal infections, it is important to understand biofilm formation of e. faecalis in the presence of other microorganisms. | 2009 | 19720224 |
| antibacterial effect of experimental chlorhexidine-releasing polymethyl methacrylate-based root canal sealers. | this study evaluated the extent of chlorhexidine release from experimental chlorhexidine-containing polymethyl methacrylate (pmma)-based root canal sealers prepared from a methacrylate-based sealer (superbond sealer, sun medical co ltd, shiga, japan) and to examine its antimicrobial effect against enterococcus faecalis using the direct contact test. | 2009 | 19720226 |
| [highly conjugative plasmids in enterococci]. | 2009 | 19721337 | |
| assay of enterocin as-48 for inhibition of foodborne pathogens in desserts. | enterocin as-48 was tested against staphylococcus aureus, bacillus cereus, and listeria monocytogenes in different kinds of desserts. the highest activity against s. aureus was detected in baker cream. however, in yogurt-type soy-based desserts and in gelatin pudding, as-48 (175 arbitrary units [au]/g) reduced viable cell counts of s. aureus by only 1.5 to 1.8 log units at most. the efficacy of as-48 in puddings greatly depended on inoculum size, and viable s. aureus counts decreased below detec ... | 2009 | 19722396 |
| expression, purification, characteristics and homology modeling of the hmgs from streptococcus pneumoniae. | to understand the molecular basis for a potential reaction mechanism and develop novel antibiotics with homology modeling for 3-hydroxy-3-methylglutaryl coenzyme a (hmg-coa) synthase (hmgs). | 2009 | 19725466 |
| evaluation of toxic side effects of clinically used skin antiseptics in vitro. | skin antiseptics are widely used in health-care worldwide. however, there is a need to determine cytotoxicity of these medications on wounds. the aim of this study was to evaluate cytotoxic effects of five clinically used antiseptics on human skin cells. | 2010 | 19726054 |
| lpxtg surface proteins of enterococci. | enterococci have become an important cause of nosocomial infections since the late 1980s. several surface proteins have been implicated in contributing to infections caused by enterococcus faecalis and enterococcus faecium. understanding the in vivo function of enterococcal surface proteins, particularly their role in directing interactions with the host during infection, is essential to explain the success of enterococci as nosocomial pathogens. here we review current knowledge of enterococcal ... | 2009 | 19726195 |
| genetic diversity of enterococci harboring the high-level gentamicin resistance gene aac(6')-ie-aph(2'')-ia or aph(2'')-ie in a japanese hospital. | prevalence of high-level gentamicin resistance genes aac(6')-ie-aph(2'')-ia and aph(2'')-ie, which encode distinct aminoglycoside-modifying enzymes, was analyzed for a total of 1128 clinical isolates of enterococci obtained in a japanese hospital during a period between 1997 and 2007. the aac(6')-ie-aph(2'')-ia was detected in 40.1%, 12.9%, and 3.6% of enterococcus faecalis, e. faecium, and other enterococcal species, respectively, and aph(2'')-ie was detected in 3.3% of e. faecium. during the s ... | 2009 | 19728776 |
| chemical composition, antifungal and antibacterial activity of the essential oil of chamaecyparis nootkatensis from spain. | the chemical composition of the oil of chamaecyparis nootkatensis (d. don) spach. has been analysed by gas chromatography (gc) and gas chromatography coupled to mass spectrometry (gc-ms). a total of 34 compounds were identified representing more than 90% of the total oil. the oil was richer in monoterpenes than in sesquiterpenes, the major constituents being limonene (53.2%), 6-3-carene (21.0%) and a-pinene (12.2%). the antibacterial and antifungal activities of the oil were also tested against ... | 2009 | 19731613 |
| bathing waters: new directive, new standards, new quality approach. | bathing water quality is an important public health issue, mainly because of fecal contamination. in 2006, the european commission (ec) adopted a new directive with respect to recreational bathing waters that calls for stricter standards and reduces the number of laboratory tests done in routine beach monitoring from nineteen to two bacterial indicators, namely, escherichia coli and intestinal enterococci, replacing policies of the ec bathing water directive that have existed since 1976. our pra ... | 2009 | 19732913 |
| in vitro activity of tigecycline and comparator agents against a global collection of gram-negative and gram-positive organisms: tigecycline evaluation and surveillance trial 2004 to 2007. | the tigecycline evaluation and surveillance trial began in 2004 to monitor the in vitro activity of tigecycline and comparator agents against a global collection of gram-negative and gram-positive pathogens. against gram negatives (n = 63 699), tigecycline mic(90)'s ranged from 0.25 to 2 mg/l for escherichia coli, haemophilus influenzae, acinetobacter baumannii, klebsiella oxytoca, enterobacter cloacae, klebsiella pneumoniae, and serratia marcescens (but was > or =32 for pseudomonas aeruginosa). ... | 2009 | 19733459 |
| review of virulence factors of enterococcus: an emerging nosocomial pathogen. | enterococcus, considered a normal commensal of intestinal tract, is fast emerging as a pathogen causing serious and life threatening hospital borne infections. this is attributed to acquisition of multi drug resistance and virulence factors of the organisms. the sequencing of enterococcus faecalis has given a lot of insight into its genetic makeup. the e. faecalis strain v583, which has been sequenced, contains a total of 3182 open reading frames (orfs) with 1760 of these showing similarity to k ... | 2009 | 19736397 |
| ecology of enterococcus faecalis and niche-adapted or non-niche-adapted enterococcus faecium in continuous-flow anaerobic cultures. | an anaerobic continuous-flow culture of chicken gastrointestinal microflora (ccf) and pure cultures of enterococcus faecalis i.2 were used for survivability studies of niche-adapted and non-niche-adapted enterococcus faecium isolates. ccf eliminated non-ccf niche-adapted glycopeptide-resistant e. faecium 47 (gre47) at a rate of 1.01 log(10) cfu/ml/day, whereas ccf niche-adapted e. faecium i.3(rif) survived in ccf at 4.5-6.5 log(10) cfu/ml. in continuous-flow monocultures of gre47 (8.93 log(10) c ... | 2009 | 19737066 |
| in vitro activity of nemonoxacin, a novel nonfluorinated quinolone, against 2,440 clinical isolates. | the in vitro activity of nemonoxacin (tg-873870), a novel nonfluorinated quinolone, was tested against 2,440 clinical isolates. nemonoxacin was at least fourfold more active than levofloxacin and moxifloxacin against most gram-positive cocci tested (shown by the following mic(90)/range [microg/ml] values; community-associated methicillin [meticillin]-resistant staphylococcus aureus, 0.5/0.015 to 2; staphylococcus epidermidis, 0.5/0.015 to 4 for methicillin-susceptible staphylococci and 2/0.12 to ... | 2009 | 19738018 |
| comparative in vitro activity profile of oritavancin against recent gram-positive clinical isolates. | oritavancin activity was tested against 15,764 gram-positive isolates collected from 246 hospital centers in 25 countries between 2005 and 2008. organisms were staphylococcus aureus (n = 9,075), coagulase-negative staphylococci (n = 1,664), enterococcus faecalis (n = 1,738), enterococcus faecium (n = 819), streptococcus pyogenes (n = 959), streptococcus agalactiae (n = 415), group c, g, and f streptococci (n = 84), and streptococcus pneumoniae (n = 1,010). among the evaluated staphylococci, 56.7 ... | 2009 | 19738026 |
| in vitro antimicrobial activity of acroseal, polifil and epiphany against enterococcus faecalis. | using the agar diffusion method, this study evaluated the in vitro antimicrobial activity of the commercial endodontic sealers acroseal and epiphany, a castor-oil based experimental sealer, polifil, and a primer agent (epiphany self-etching primer), against enterococcus faecalis. zinc oxide and eugenol cement (zoe) served as control. five wells per dish were made at equidistant points and immediately filled with the test and control materials. after incubation of the dishes at 37 degrees c for 2 ... | 2009 | 19738941 |
| identification and isolation of an azoreductase from enterococcus faecium. | azo dyes are commonly used in many commercial industries. some of the azo dyes can produce carcinogenic compounds after being metabolized by azoreductase. several human intestinal microbiota possess azoreductase activity which plays an important role in the toxicity and mutagenicity of these azo dye compounds. the acpd gene product (azoef1) responsible for the azoreductase activity of enterococcus faecium, an intestinal bacterium, was heterologously expressed, purified and characterized. the pro ... | 2010 | 19741272 |
| concerning the survival of saliva-borne oral bacteria on patient notes. | 2009 | 19748156 | |
| antimicrobial susceptibility of gram-positive bacteria isolated from us medical centers: results of the daptomycin surveillance program (2007-2008). | gram-positive bacterial strains (12 443) consecutively collected during 2007 to 2008 in hospitals located in the united states were tested by reference broth microdilution methods against daptomycin and comparison agents. methicillin (oxacillin) resistance rates were 55.9% and 74.0% for staphylococcus aureus and coagulase-negative staphylococci, respectively, and the vancomycin resistance rate among enterococcus faecalis and enterococcus faecium were 5.4% and 75.4%, respectively. daptomycin was ... | 2009 | 19748426 |
| in vivo activity of a novel anti-methicillin-resistant staphylococcus aureus cephalosporin, ceftaroline, against vancomycin-susceptible and -resistant enterococcus faecalis strains in a rabbit endocarditis model: a comparative study with linezolid and vancomycin. | we assessed the in vitro and in vivo efficacy of the novel parenteral broad-spectrum cephalosporin ceftaroline against enterococcus faecalis in time-kill experiments and in a rabbit endocarditis model with simulated human dosing. ceftaroline was more active than either vancomycin or linezolid against vancomycin-sensitive and -resistant isolates of e. faecalis. | 2009 | 19752276 |
| microbiology of drugs for treating multiply drug-resistant gram-positive bacteria. | several new antimicrobials demonstrate in vitro activity against methicillin-resistant staphylococcus aureus (mrsa), vancomycin-resistant enterococci (vre), and other gram-positive bacteria. data from large surveys indicate that linezolid, daptomycin, and tigecycline are almost universally active against mrsa. linezolid and tigecycline inhibit both enterococcus faecium and enterococcus faecalis at low concentrations; daptomycin is somewhat more potent against the latter. the investigational agen ... | 2009 | 19766885 |
| chemical composition and antibacterial activity of the essential oil of cordia verbenacea from the venezuelan andes. | the essential oil of cordia verbenacea d.c. (boraginaceae) that grows in mérida-venezuela was obtained by hydrodistillation from the aerial parts of the plant, yielding 0.21%. the oil was analyzed by gc-fid and gc-ms. thirty one components which made up 94.3% of the oil were identified. the most abundant constituents found were: tricyclene (23.9%), bicyclogermacrene (11.7%), germacrene d (9.9%) and beta-caryophyllene (8.2%). antibacterial activity determination was carried out according to the d ... | 2009 | 19768996 |
| [effect of enterococcus faecalis on apoptosis rate and the release of matrix metalloproteinase-8 of polymorphonuclear leukocytes]. | to evaluate the release of matrix metalloproteinase-8 (mmp-8) and apoptosis rate of polymorphonuclear leukocytes (pmns) after pmns was triggered by enterococcus faecalis (e. faecalis) in vitro. | 2009 | 19769269 |
| antimicrobial and antioxidant activities of plants from northeast of mexico. | traditional medicine has a key role in health care worldwide. obtaining scientific information about the efficacy and safety of the plants from our region is one of the goals of our research group. in this report, 17 plants were selected and collected in different localities from northeast mexico. the dried plants were separated into leaves, flowers, fruit, stems, roots and bark. each part was extracted with methanol, and 39 crude extracts were prepared. the extracts were tested for their antimi ... | 2009 | 19770266 |
| is there an association between nosocomial infection rates and bacterial cross transmissions? | surveillance data of nosocomial infection rates are increasingly used for public reporting and interhospital comparisons. approximately 15% of nosocomial infections on intensive care units are the result of patient-to-patient transmissions of the causative organisms. these exogenous infections could be prevented by adherence to basic infection control measures. the association between bacterial cross transmissions and nosocomial infection rates was analyzed. | 2010 | 19770743 |
| freezing inactivation of escherichia coli and enterococcus faecalis in water: response of different strains. | the effect of freezing temperature (-7, -15, -30 and -80 degrees c), number of freeze/thaw cycles (1 to 5 cycles) and sample volume (100 ml and 100 microl) on the viability of a pathogenic and an opportunistically pathogenic escherichia coli, a vancomycin-resistant and a vancomycin-sensitive enterococcus faecalis were examined. about 3.3 to 4.3 and 1.5 to 2.4 log reduction in cell density were observed in e. coli and e. faecalis, respectively, in the 100 ml samples frozen at -30 degrees c or war ... | 2009 | 19774859 |
| comparative evaluation of antimicrobial activity of three cements: new endodontic cement (nec), mineral trioxide aggregate (mta) and portland. | using the agar diffusion method, we conducted an in vitro study to evaluate the antimicrobial activity of mineral trioxide aggregate (mta), new endodontic cement (nec) and portland cement at different concentrations against five different microorganisms. a base layer was made using muller-hinton agar for escherichia coli (atcc 10538) and candida (atcc 10231). for actinomyces viscosus (atcc 15987), enterococcus faecalis (atcc 10541) and streptococcus mutans (atcc 25175) blood agar medium was used ... | 2009 | 19776512 |
| total synthesis and biological evaluation of (+)- and (-)-bisanthraquinone antibiotic be-43472b and related compounds. | the bisanthraquinone antibiotic be-43472b [(+)-1] was isolated by rowley and co-workers from a streptomycete strain found in a blue-green algae associated with the ascidian ecteinascidia turbinata and has shown promising antibacterial activity against clinically derived isolates of methicillin-susceptible, methicillin-resistant, and tetracyclin-resistant staphylococcus aureus (mssa, mrsa, and trsa, respectively) and vancomycin-resistant enterococcus faecalis (vre). described herein is the first ... | 2009 | 19778008 |
| characterization of enterococci populations collected from a subsurface flow constructed wetland. | the aim of this study was to identify and characterize the population of enterococcus sp. in domestic wastewater as it flows through a constructed wetland. | 2010 | 19778344 |
| influence of oral hygiene in patients with fixed appliances in the oral carriage of antimicrobial-resistant escherichia coli and enterococcus isolates. | the aim was to study the oral carriage of enterococcus and escherichia coli isolates and their content in antimicrobial-resistance and virulence genes in patients with fixed appliances and in healthy volunteers. | 2009 | 19778742 |
| [occurrence of virulence genes among enterococcus faecalis strains isolated from patients and hospital environment]. | the aim of this study was to evaluate occurrence of virulence genes among enterococcus faecalis strains isolated from two hospitals in łódź, during 2005-2006. the second goal was to determine possible relationship between presence of those genes, resistance to ampicillin and sources of isolation. enterococcal strains were identified to the species by pcr with ddl primers. all 161 isolates were tested for the presence of aggregation substance gene (agg), cytolisine genes (cyl-l, cyl-s), esp prote ... | 2009 | 19780490 |
| bacteriology of external ocular infections in aba, south eastern nigeria. | bacteria are microbial agents that frequently cause infections of the eye and possible loss of vision. | 2009 | 19780761 |
| effect of dosing interval on the efficacy of topical ophthalmic gatifloxacin against enterococcus faecalis in an in vitro pharmacokinetic model simulating the local eye compartment. | improved dosing regimens have been proposed for various antimicrobial agents by application of pharmacokinetic/pharmacodynamic (pk/pd) principles. however, for topical ophthalmic use there are several limitations to changing the dosing regimen, such as drug formulation and bioavailability. in this study, we investigated the relationship between dosing interval and antibacterial efficacy in an in vitro pk model mimicking post-operative endophthalmitis. the in vitro pk model simulated the aqueous ... | 2009 | 19782539 |