Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| adenylate cyclase orthologues in two filamentous entomopathogens contribute differentially to growth, conidiation, pathogenicity, and multistress responses. | adenylate cyclase (ac) is a core element of camp signalling network. here we show functional diversity and differentiation of beauveria bassiana ac (bbac) and metarhizium robertsii ac (mrac). severe growth defects occurred in δbbac and δmrac grown on nutrition-rich sday and several minimal media but were largely alleviated by adding camp to sday. conidial yield increased greatly in δbbac but decreased in δmrac. during colony growth, δbbac was highly sensitive to oxidation, high osmolarity, cell ... | 2014 | 24742837 |
| development and evaluation of a novel contamination device that targets multiple life-stages of aedes aegypti. | the increasing global threat of dengue demands new and easily applicable vector control methods. ovitraps provide a low-tech and inexpensive means to combat dengue vectors. here we describe the development and optimization process of a novel contamination device that targets multiple life-stages of the aedes aegypti mosquito. special focus is directed to the diverse array of control agents deployed in this trap, covering adulticidal, larvicidal and autodissemination impacts. | 2014 | 24766772 |
| specific diversity of the entomopathogenic fungi beauveria and metarhizium in mexican agricultural soils. | prior knowledge of the local population structure of entomopathogenic fungi is considered an important requisite when developing microbial control strategies against major pests of crops such as white grubs. an extensive survey in the estate of guanajuato, one of the main agricultural regions of mexico, was carried out to determine the abundance and diversity of entomopathogenic fungi in soil. soil collected from 11 locations was baited for entomopathogenic fungi using galleria mellonella. in ad ... | 2014 | 24769124 |
| a novel approach: beauveria bassiana granules applied to nursery soil for management of rice water weevils in paddy fields. | spraying of entomopathogenic fungi formulated as wettable powders and suspension concentrates based on oils or water has been used for the management of crop pests. however, the use of fungal granules to control paddy field pests has not been fully explored. herein, several beauveria bassiana isolates (erl1170, erl1578 and erl836) were produced as granules using a millet-based solid culture. the granules were applied to the rice nursery 3 days before transplanting, and their control efficacy aga ... | 2014 | 24771507 |
| sustainable management tactics for control of phyllotreta cruciferae (coleoptera: chrysomelidae) on canola in montana. | the crucifer flea beetle, phyllotreta cruciferae (goeze) (coleoptera: chrysomelidae), has recently emerged as a serious pest of canola (brassica napus l.) in montana. the adult beetles feed on canola leaves, causing many small holes that stunt growth and reduce yield. in 2013, damage to canola seedlings was high (approximately 80%) in many parts of montana, evidence that when flea beetles emerge in large numbers, they can quickly destroy a young canola crop. in the current study, the effectivene ... | 2014 | 24772547 |
| [identification of spore germination and virulence related biomarkers from beauveria bassiana using an lc-ms-based metabolomic technique]. | to identify biomarkers associated with germination and virulence of beauveria bassiana. | 2014 | 24783852 |
| mycosis inhibits cannibalism by melanoplus sanguinipes, m. differentialis, schistocerca americana, and anabrus simplex. | cannibalism is common among the acrididae and the mormon cricket, anabrus simplex haldeman (orthoptera: tettigoniidae). this behavior has been proposed as a mechanism for the horizontal transmission of microsporida and entomopathogenic fungi. aanecdotal observations suggested that the migratory grasshopper, melanoplus sanguinipes fabricius (acrididae), and a. simplex did not eat cadavers that had been killed by insect pathogenic fungi. the hypothesis tested was that a. simplex or m. sanguinipes ... | 2013 | 24786183 |
| differentially expressed genes in the cuticle and hemolymph of the silkworm, bombyx mori, injected with the fungus beauveria bassiana. | the most important pathogenic fungus of the silkworm, bombyx mori l. (lepidoptera: bombycidae), is beauveria bassiana (balsamo-crivelli ) vuillemin (hypocreales: clavicipitaceae), which causes significant damage to sericulture production. therefore, diagnosing fungal disease and developing new control measures are crucial to silk production. to better understand the responsive and interactive mechanisms between the host silkworm and this fungus, variations in silkworm gene expression were invest ... | 2013 | 24794288 |
| reduced survival and reproductive success generates selection pressure for the dengue mosquito aedes aegypti to evolve resistance against infection by the microsporidian parasite vavraia culicis. | the success and sustainability of control measures aimed at reducing the transmission of mosquito-borne diseases will depend on how they influence the fitness of mosquitoes in targeted populations. we investigated the effects of the microsporidian parasite vavraia culicis on the survival, blood-feeding behaviour and reproductive success of female aedes aegypti mosquitoes, the main vector of dengue. infection reduced survival to adulthood and increased adult female mosquito age-dependent mortalit ... | 2014 | 24822081 |
| expression of a toll signaling regulator serpin in a mycoinsecticide for increased virulence. | serpins are ubiquitously distributed serine protease inhibitors that covalently bind to target proteases to exert their activities. serpins regulate a wide range of activities, particularly those in which protease-mediated cascades are active. the drosophila melanogaster serpin spn43ac negatively controls the toll pathway that is activated in response to fungal infection. the entomopathogenic fungus beauveria bassiana offers an environmentally friendly alternative to chemical pesticides for inse ... | 0 | 24837378 |
| myco-synthesis of silver nanoparticles using beauveria bassiana against dengue vector, aedes aegypti (diptera: culicidae). | the efficacy of silver synthesized biolarvicide with the help of entomopathogenic fungus, beauveria bassiana, was assessed against the different larval instars of dengue vector, aedes aegypti. the silver nanoparticles were observed and characterized by a scanning electron microscope (sem) and energy-dispersive x-ray (edx). a surface plasmon resonance band was observed at 420 nm in uv-vis spectrophotometer. the characterization was confirmed by shape (spherical), size 36.88-60.93 nm, and edx spec ... | 2014 | 24861012 |
| direct plantlet inoculation with soil or insect-associated fungi may control cabbage root fly maggots. | a potential delia radicum biological control strategy involving cauliflower plantlet inoculation with various fungi was investigated in a series of laboratory and glasshouse experiments. in addition to entomopathogenic fungi, fungi with a high rhizosphere competence and fungi with the ability to survive as saprotrophs in soil were tested. the following fungal species were evaluated in the experiments: trichoderma atroviride, t. koningiopsis, t. gamsii, beauveria bassiana, metharhizium anisopliae ... | 2014 | 24907449 |
| wee1 and cdc25 control morphogenesis, virulence and multistress tolerance of beauveria bassiana by balancing cell cycle-required cyclin-dependent kinase 1 activity. | modification of cell cycle in entomopathogenic fungi is likely crucial for host infection and environmental adaptation. here we show that wee1 and cdc25 can balance cell cycle-required cyclin-dependent kinase 1 (cdk1) activity in beauveria bassiana. the cdk1 phosporylation signal was strong in δcdc25 but very weak in δwee1 and absent in δwee1δcdc25. consequently, cell cycles, septation patterns and many septation-dependent gene transcripts of these mutants were reversely changed. hyphal cells we ... | 2015 | 24910927 |
| effect of fungal colonization of wheat grains with fusarium spp. on food choice, weight gain and mortality of meal beetle larvae (tenebrio molitor). | species of fusarium have significant agro-economical and human health-related impact by infecting diverse crop plants and synthesizing diverse mycotoxins. here, we investigated interactions of grain-feeding tenebrio molitor larvae with four grain-colonizing fusarium species on wheat kernels. since numerous metabolites produced by fusarium spp. are toxic to insects, we tested the hypothesis that the insect senses and avoids fusarium-colonized grains. we found that only kernels colonized with f. a ... | 2014 | 24932485 |
| three types of geranylgeranyl diphosphate synthases from the medicinal caterpillar fungus, cordyceps militaris (ascomycetes). | geranylgeranyl diphosphate synthase (ggpps) is a key enzyme in the carotenoid biosynthetic pathway, catalyzing the synthesis of its c20 precursor. in the present study, three types of ggpps genes were cloned and analyzed from the caterpillar medicinal fungus cordyceps militaris, a valued carotenoid-producing species. the sequences were named as ggpps727, ggpps191, and ggpps595. the open reading frame codes for predicted polypeptides of 464, 550, and 431 aa. three predicted ggppss had a high simi ... | 2014 | 24941033 |
| evaluation of the effectiveness of entomopathogens for the management of wireworms (coleoptera: elateridae) on spring wheat. | wireworms, the larval stage of click beetles (coleoptera: elateridae), are serious soil dwelling pests of small grains, corn, sugar beets, and potatoes. limonius californicus and hypnoidus bicolor are the predominant wireworm species infesting wheat in montana, particularly in the 'golden triangle' area of north-central montana. wireworm populations in field crops are increasing, but currently available insecticides provide only partial control, and no alternative management tools exist. in our ... | 2014 | 24944009 |
| screening of liquid media and fermentation of an endophytic beauveria bassiana strain in a bioreactor. | a novel approach for biological control of insect pests could be the use of the endophytic entomopathogenic beauveria bassiana isolate atp-02. for the utilization of the endophyte as a commercial biocontrol agent, the fungus has to be mass-produced. b. bassiana was raised in shake flask cultures to produce high concentrations of total spores (ts), which include blastospores (bs) and submerged conidiospores (scs). the highest concentration of 1.33×10(9) ts/ml and the highest yield of 5.32×10(10) ... | 2014 | 24949278 |
| immune priming and pathogen resistance in ant queens. | growing empirical evidence indicates that invertebrates become more resistant to a pathogen following initial exposure to a nonlethal dose; yet the generality, mechanisms, and adaptive value of such immune priming are still under debate. because life-history theory predicts that immune priming and large investment in immunity should be more frequent in long-lived species, we here tested for immune priming and pathogen resistance in ant queens, which have extraordinarily long life span. we expose ... | 2014 | 24963375 |
| bbmsn2 acts as a ph-dependent negative regulator of secondary metabolite production in the entomopathogenic fungus beauveria bassiana. | fungal secondary metabolites are chemical compounds important for development, environmental adaptation and for potential biotechnological and pharmaceutical applications. oosporein, a red-pigmented benzoquinone, produced by many fungal insect pathogenic beauveria spp., shows remarkable functional diversity, displaying antimicrobial, antiviral and even anti-proliferative activities. a homologue of the msn2/seb1 transcription factor was identified in a beauveria bassiana random t-dna insertion li ... | 2015 | 24965521 |
| three α-1,2-mannosyltransferases contribute differentially to conidiation, cell wall integrity, multistress tolerance and virulence of beauveria bassiana. | members of α-1,2-mannosyltransferase (ktr) family are required for protein o-mannosylation for the elongation of ser/thr mannose residues in yeasts but functionally unknown in most filamentous fungi. here we characterized the functions of the ktr orthologues ktr1, ktr4 and kre2/mnt1 in beauveria bassiana, a filamentous enotmopathogen, and found that they were positive, but differential, mediators of many biological traits. inactivation of ktr4 and kre2 resulted in 92% reduction of conidial yield ... | 2014 | 24981201 |
| pathogenicity bioassays of isolates of beauveria bassiana on rhynchophorus ferrugineus. | the control of rhynchophorus ferrugineus (olivier), the main palm pest in the mediterranean basin, is problematic because of its biology and the current restrictions in many european countries on the use of chemical insecticides in urban areas. entomopathogenic fungi have been studied as potential biological control agents, but information on their natural incidence is limited. strains of beauveria bassiana (balsamo) vuillemin were isolated from symptomatic insects collected on dead palms, and t ... | 2015 | 24990249 |
| a serine protease homolog negatively regulates tep1 consumption in systemic infections of the malaria vector anopheles gambiae. | clip domain serine protease homologs are widely distributed in insect genomes and play important roles in regulating insect immune responses, yet their exact functions remain poorly understood. here, we show that clipa2, a clip domain serine protease homolog of anopheles gambiae, regulates the consumption of the mosquito complement-like protein tep1 during systemic bacterial infections. we provide evidence that clipa2 localizes to microbial surfaces in a tep1-dependent manner whereby it negative ... | 2014 | 25012124 |
| bbsnf1 contributes to cell differentiation, extracellular acidification, and virulence in beauveria bassiana, a filamentous entomopathogenic fungus. | snf1/ampk protein kinase plays important roles in fungal development and activation of catabolite-repressed genes. in this study, we characterized the role of the beauveria bassiana snf1 ortholog. the vegetative growth of the δbbsnf1 mutant was reduced by 16 to 50 % on diverse carbon/nitrogen sources. transcriptional analysis revealed a collection of proteases and chitinases that were not induced when the mutant was grown on complex carbon/nitrogen sources. bbsnf1 also contributes to extracellul ... | 2014 | 25016341 |
| interactions between the entomopathogenic fungi beauveria bassiana (ascomycota: hypocreales) and the aphid parasitoid diaeretiella rapae (hymenoptera: braconidae) on myzus persicae (hemiptera: aphididae). | the interactions between the entomopathogenic fungus beauveria bassiana (balsamo-crivelli) vuillemin (ascomycota: hypocreales) and the aphid parasitoid diaeretiella rapae mcintoch (hymenoptera: braconidae) were evaluated under laboratory conditions. nymphs of myzus persicae sulzer (hemiptera: aphididae) were first exposed to parasitoid females for 24 h and then 0, 24, and 48 h afterwards sprayed with a solution of b. bassiana. likewise, aphids were also sprayed with b. bassiana and then exposed ... | 2014 | 25026650 |
| s-(-)-10,11-dihydroxyfarnesoic acid methyl ester inhibits melanin synthesis in murine melanocyte cells. | the development of antimelanogenic agents is important for the prevention of serious aesthetic problems such as melasmas, freckles, age spots, and chloasmas. in the course of screening for melanin synthesis inhibitors, we found that the culture broth from an insect morphopathogenic fungus, beauveria bassiana cs1029, exhibits potent antimelanogenic activity. we isolated and purified an active metabolite and identified it as s-(-)-10,11-dihydroxyfarnesoic acid methyl ester (dhfame), an insect juve ... | 2014 | 25046747 |
| comparison of in vitro and in vivo phototoxicity tests with s-(-)-10,11-dihydroxyfarnesic acid methyl ester produced by beauveria bassiana kacc46831. | beauveria bassiana is a fungi that is well-known for demonstrating a resistance to environmental change. to confirm whether s-(-)-10,11-dihydroxyfarnesic acid methyl ester (dhfame) produced by beauveria bassiana kacc46831 causes phototoxicity when used for cosmetic purposes due to its anti-tyrosinase activity, we conducted in vitro and in vivo phototoxicity tests. there were no significant changes or damage observed in the compound-treated group with regards to skin phototoxicity, while 8-methox ... | 2014 | 25054007 |
| antifungal activity of stenotrophomonas maltophilia in stomoxys calcitrans larvae. | the microbiota present in stomoxys calcitrans larvae may assist their survival in contaminated environments through production of inhibitory substances. bacteriological identification methods, the polymerase chain reaction (pcr) and scanning electron microscopy (sem) were used to detect a bacterium naturally present in mucus and macerated s. calcitrans larvae. the antifungal activity was determined based on the results from disk diffusion tests on an artificial solid medium. the bacterium was id ... | 2014 | 25054498 |
| enhanced conidial thermotolerance and field efficacy of beauveria bassiana by saccharides. | insecticide efficacy of beauveria bassiana conidia was improved by optimizing the concentrations of conidial heat-protective saccharides (glucose, sucrose, maltose, trehalose, α-lactose, and mannitol) using response surface methodology. two field trials in tea gardens were carried out to control leafhopper (empoasca vitis) by spraying b. bassiana conidia together with the optimized saccharides (0.26 g glucose, 0.28 g lactose, 0.24 g mannitol per ml). in the field studies, b. bassiana conidia wer ... | 2014 | 25062642 |
| prevalence of beauveria pseudobassiana among entomopathogenic fungi isolated from the hard tick, ixodes ricinus. | human and animal disease-transmitting hard ticks (acari: ixodidae) are of great concern for public health and animal farming. alternatives to tick control by chemical acaricides are urgently needed, and one intensively evaluated biocontrol strategy is based on the use of tick-pathogenic filamentous fungi. an indispensable prerequisite for the development of tick-derived fungal isolates into registered myco-acaricides is their sound taxonomic characterisation. a set of fungal strains isolated fro ... | 2014 | 25065606 |
| microbial metabolism of danazol: a contribution to doping analysis. | microbial metabolism of danazol (17alpha-pregna-2,4-dien-20-yno[2,3-d]isoxazol-17beta-ol) by beauveria bassiana atcc 7159 and glyocladium viride atcc 10097 afforded four metabolites. the isolated metabolites were identified by different spectroscopic techniques as 6beta-hydroxy danazol, which is a not yet reported danazol metabolite, 17beta-hydroxy-17alpha-pregn-4-en-20-yn-3-one (ethisterone) and 17beta-hydroxy-2alpha-(hydroxymethyl)-17alpha-pregn-4-en-20-yn-3-one (2alpha-hydroxymethyl ethistero ... | 2014 | 25069163 |
| occurrence of entomopathogenic fungi in tejocote (crataegus mexicana) orchard soils and their pathogenicity against rhagoletis pomonella. | to determine the abundance and diversity of entomopathogenic fungi in tejocote orchard soils and evaluate their ability to infect rhagoletis pomonella walsh., the main pest of tejocote. | 2014 | 25081747 |
| the entomopathogenic fungal endophytes purpureocillium lilacinum (formerly paecilomyces lilacinus) and beauveria bassiana negatively affect cotton aphid reproduction under both greenhouse and field conditions. | the effects of two entomopathogenic fungal endophytes, beauveria bassiana and purpureocillium lilacinum (formerly paecilomyces lilacinus), were assessed on the reproduction of cotton aphid, aphis gossypii glover (homoptera:aphididae), through in planta feeding trials. in replicate greenhouse and field trials, cotton plants (gossypium hirsutum) were inoculated as seed treatments with two concentrations of b. bassiana or p. lilacinum conidia. positive colonization of cotton by the endophytes was c ... | 2014 | 25093505 |
| dual function of a bee (apis cerana) inhibitor cysteine knot peptide that acts as an antifungal peptide and insecticidal venom toxin. | inhibitor cysteine knot (ick) peptides exhibit ion channel blocking, insecticidal, and antimicrobial activities, but currently, no functional roles for bee-derived ick peptides have been identified. in this study, a bee (apis cerana) ick peptide (acick) that acts as an antifungal peptide and as an insecticidal venom toxin was identified. acick contains an ick fold that is expressed in the epidermis, fat body, or venom gland and is present as a 6.6-kda peptide in bee venom. recombinant acick pept ... | 2014 | 25106915 |
| a molecular tool for detection and tracking of a potential indigenous beauveria bassiana strain for managing emerald ash borer populations in canada. | emerald ash borer is an invasive species from asia. beauveria bassiana strain l49-1aa is being tested for the control of emerald ash borer in canada, using an autocontamination trapping system. we have developed a simplified allele discrimination polymerase chain reaction (pcr) assay to screen b. bassiana strain, l49-1aa from other beauveria species by targeting the inter-strain genetic differences in 5' end of ef1-α gene of the genus beauveria. a single nucleotide polymorphism (snp) site, t→c w ... | 2014 | 25110340 |
| laboratory and field efficacy of entomopathogenic fungi for the management of the sweetpotato weevil, cylas formicarius (coleoptera: brentidae). | the sweetpotato weevil, cylas formicarius (f.) (coleoptera: brentidae), is one of the most important pests of sweet potatoes in the world. with free trade between the united states and the u.s.-controlled mariana islands, c. formicarius has spread along with this commodity. because of the cryptic nature of the larvae and nocturnal activity of the adults, and the cancellation of long-residual pesticides, this pest has become increasingly difficult to control. therefore, the present study sought t ... | 2014 | 25111763 |
| toxicity of naturally occurring bio-fly and chitosan compounds to control the mediterranean fruit fly ceratitis capitata (wiedemann). | the efficacy of five compounds of a biopolymer chitosan and bio-fly (beauveria bassiana fungus) as biopesticide was evaluated on ceratitis capitata under laboratory conditions. the inhibitory effects on acetylcholinesterase (ache) and adenosinetriphosphatase (atpase) as biochemical indicators were also determined in vivo. the results indicated that b. bassiana based bio-fly exhibited significant toxicity against c. capitata (lc50 = 3008 and 3126 mg/l after 48 h in females and males, respectively ... | 2015 | 25117027 |
| foster carers influence brood pathogen resistance in ants. | social organisms face a high risk of epidemics, and respond to this threat by combining efficient individual and collective defences against pathogens. an intriguing and little studied feature of social animals is that individual pathogen resistance may depend not only on genetic or maternal factors, but also on the social environment during development. here, we used a cross-fostering experiment to investigate whether the pathogen resistance of individual ant workers was shaped by their own col ... | 2014 | 25143036 |
| a putative methyltransferase, mtra, contributes to development, spore viability, protein secretion and virulence in the entomopathogenic fungus beauveria bassiana. | the filamentous fungus, beauveria bassiana, is a ubiquitously distributed insect pathogen, currently used as an alternative to chemical pesticides for pest control. conidiospores are the means by which the fungus disseminates in the environment, and these cells also represent the infectious agent most commonly used in field applications. little, however, is known concerning the molecular basis for maintenance of spore viability, a critical feature for survival and persistence. here, we report on ... | 2014 | 25194143 |
| evaluation of insect associated and plant growth promoting fungi in the control of cabbage root flies. | delia radicum l. or cabbage maggot is an important pest for brassicaceous crops. there are currently no registered chemical control agents for its control in slovenia. fungal control agents for cabbage maggot were therefore sought among nine rhizosphere-compatible and plant growth-promoting, soil-adapted, and entomopathogenic species to cabbage maggots and were assayed in in vitro and soil laboratory bioassays. in the in vitro tests, the conidial suspensions were applied directly to cabbage magg ... | 2014 | 25195421 |
| diversity of soil fungi in north 24 parganas and their antagonistic potential against leucinodes orbonalis guen. (shoot and fruit borer of brinjal). | soil samples were collected from agricultural fields and gardens in north 24 parganas, west bengal, and fungi species were isolated from them. thirty-one fungal species were isolated with 19 found in agricultural soil and 28 in garden soil. twenty-eight out of 31 were identified using cultural and microscopic characters, and three were unidentified. the diversity of isolated fungi was calculated by simpson's diversity index. the garden soil possessed more fungal colonies (750) than agricultural ... | 2014 | 25213561 |
| lethal effects of a mexican beauveria bassiana (balsamo) strain against meccus pallidipennis (stal). | the entomopathogenic fungus beauveria bassiana (balsamo 1835) vuillemin is an effective alternative control agent against some agricultural pests and biological vectors of important diseases such as chagas disease. in this work we studied an isolate of beauveria bassiana from of the town of san antonio rayón, puebla, mexico and its entomopathogenic effects on meccus pallidipennis (stal 1872). phylogenetic analysis using molecular comparison of the its and ef1α genes, showed that the resulting cl ... | 2014 | 25242941 |
| [antagonism against beauveria bassiana by lipopeptide metabolites produced by entophyte bacillus amyloliquefaciens strain swb16]. | we screened bacterial strains that have strong antagonism against beauveria bassiana, an important pathogen of silkworm industry, and detected the antagonistic activity of lipopeptide metabolites. | 2014 | 25252459 |
| five vacuolar ca(2+) exchangers play different roles in calcineurin-dependent ca(2+)/mn(2+) tolerance, multistress responses and virulence of a filamentous entomopathogen. | multiple vcx1 (vacuolar calcium exchanger) paralogues exist in many filamentous fungi but are functionally unexplored unlike a single vcx1 ortholog well characterized in yeasts. here we show that five vcx1 paralogues (vcx1a-e) in beauveria bassiana are conditionally functional for intracellular ca(2+) homeostasis and contribute differentially to multistress tolerance and virulence in the filamentous entomopathogen. each vcx1 deletion drastically upregulated transcriptional expressions of four ot ... | 2014 | 25256588 |
| fungal keratitis caused by beauveria bassiana: drug and temperature sensitivity profiles: a case report. | beauveria bassiana is an entomopathogenic fungus and is a rare cause of keratitis. we present a case of fungal keratitis caused by b. bassiana that was diagnosed by in vivo confocal microscopy and in vitro corneal cultures. in addition, we determined the temperature- and drug-sensitivities of the isolated strain of b. bassiana. | 2014 | 25260975 |
| distinct contributions of one fe- and two cu/zn-cofactored superoxide dismutases to antioxidation, uv tolerance and virulence of beauveria bassiana. | beauveria bassiana, a filamentous entomopathogen, has five distinct superoxide dismutases (sods), including cytosolic and mitochondrial mnsods (sod2/3) which have proved contributing primarily to intracelluar sod activity and additively to antioxidation and virulence. here we characterized cytosolic cu/znsod (sod1), mitochondrial fesod (sod4) and cell wall-anchored cu/znsod (sod5). the latter two are unexplored despite existence in many filamentous fungi, and their subcellular localization was w ... | 2015 | 25263710 |
| oxidative stress in entomopathogenic fungi grown on insect-like hydrocarbons. | entomopathogenic fungi mostly attack their insect hosts by penetration through the cuticle. the outermost insect surface is covered by a lipid-rich layer, usually composed of very long chain hydrocarbons. these fungi are apt to grow on straight chain hydrocarbons (alkanes) as the sole carbon source. insect-like hydrocarbons are first hydroxylated by a microsomal p450 monooxygenase system, and then fully catabolized by peroxisomal β-oxidation reactions in beauveria bassiana. in this review, we wi ... | 2015 | 25274493 |
| unveiling equal importance of two 14-3-3 proteins for morphogenesis, conidiation, stress tolerance and virulence of an insect pathogen. | two conserved 14-3-3 proteins orthologous to saccharomyces cerevisiae bmh1/2 are poorly understood in filamentous fungi. here we show that bmh1 and bmh2 contribute equally to the fundamental biology and physiology of beauveria bassiana by targeting many sets of proteins/enzymes. single bmh deletion caused similar upregulation of another. excellent knockdown (∼91%) expressions of bmh1 in δbmh2 and bmh2 in δbmh1 resulted in equally more severe multiphenotypic defects than the single deletions, inc ... | 2015 | 25315061 |
| the role of three calcineurin subunits and a related transcription factor (crz1) in conidiation, multistress tolerance and virulence in beauveria bassiana. | the eukaryotic calcineurin (cn) pathway comprising catalytic a (cna) and regulatory b subunits (cnb) is crucial for many biological processes but functionally unexplored in entomopathogenic fungi. here, we characterise three cn subunits (cna1, cna2 and cnb) and a downstream cn-responsive zinc finger transcription factor (crz1) in beauveria bassiana. cn-mediated phosphatase activity decreased by 16-38 % in all deletion mutants compared with wild type. growth and conidiation were most defective in ... | 2015 | 25324131 |
| effects of endophyte colonization of vicia faba (fabaceae) plants on the life-history of leafminer parasitoids phaedrotoma scabriventris (hymenoptera: braconidae) and diglyphus isaea (hymenoptera: eulophidae). | effects of the fungal endophytes beauveria bassiana (isolates icipe 279, g1lu3, s4su1) and hypocrea lixii (isolate f3st1) on the life-history of phaedrotoma scabriventris and diglyphus isaea, parasitoids of the pea leafminer liriomyza huidobrensis, were studied in the laboratory. parasitoids were allowed to parasitize 2(nd) and 3(rd) instar l. huidobrensis larvae reared on endophytically-inoculated faba bean, vicia faba. in the control, parasitoids were reared on non-inoculated host plants. para ... | 2014 | 25338084 |
| physiological changes in rhipicephalus microplus (acari: ixodidae) experimentally infected with entomopathogenic fungi. | carbohydrate metabolism plays an important role in the physiology and maintenance of energy stores within living organisms. however, when organisms are exposed to adverse physiological conditions, such as during pathogenic infection, these organisms begin to use alternative substrates (proteins and lipids) for energy production. this paper studied the carbohydrate metabolism of rhipicephalus microplus after infection with beauveria bassiana and metarhizium anisopliae. the parameters evaluated we ... | 2015 | 25346195 |
| a case of beauveria bassiana keratitis confirmed by internal transcribed spacer and lsu rdna d1-d2 sequencing. | we describe a case of fungal keratitis due to beauveria bassiana in a farmer with fuchs' dystrophy, treated with amphotericin b. surgery with penetrating keratoplasty was necessary to resolve the lesions. susceptibility testing and molecular sequencing permitted the identification and treatment of this rare aetiological agent of invasive fungal disease. | 2014 | 25356350 |
| a novel bioassay to evaluate the potential of beauveria bassiana strain ni8 and the insect growth regulator novaluron against lygus lineolaris on a non-autoclaved solid artificial diet. | a non-autoclaved solid diet was used to evaluate the entomopathogenic fungus beauveria bassiana (balsamo) vuillemin (hypocreales: clavicipitaceae) strain ni8 and the insect growth regulator novaluron (diamond® 0.83ec insecticide) for control of the tarnished plant bug, lygus lineolaris (palisot de beauvois) (hemiptera: miridae). the diet was composed of toasted wheat germ, ground lima bean meal, soy flour, yolk of chicken eggs, inhibitor, and agar. it was prepared in one step by blending the ing ... | 2014 | 25368059 |
| susceptibility of adults of the cerambycid beetle hedypathes betulinus to the entomopathogenic fungi beauveria bassiana, metarhizium anisopliae, and purpureocillium lilacinum. | the cerambycid beetle hedypathes betulinus (klug) (coleoptera: cerambycidae) causes severe damage to yerba mate plants (ilex paraguariensis (st. hilaire) (aquifoliales: aquifoliaceae)), which results in large losses of production. in this study, the pathogenicity of entomopathogenic fungi of the species beauveria bassiana (balsamo-crivelli) vuillemin (hypocreales: cordycipitaceae), metarhizium anisopliae sensu lato (metschnikoff) sorokin (hypocreales: clavicipitaceae), and purpureocillium lilaci ... | 2014 | 25368071 |
| functional analysis of insect molting fluid proteins on the protection and regulation of ecdysis. | molting fluid accumulates between the old and new cuticles during periodical ecdysis in ecdysozoa. natural defects in insect ecdysis are frequently associated with melanization (an immunity response) occurring primarily in molting fluids, suggesting that molting fluid may impact immunity as well as affect ecdysis. to address this hypothesis, proteomic analysis of molting fluids from bombyx mori during three different types of ecdysis was performed. many proteins were newly identified, including ... | 2014 | 25368323 |
| selection of beauveria isolates pathogenic to adults of nilaparvata lugens. | the brown planthopper, nilaparvata lugens stål (hemiptera: delphacidae), is a destructive invasive pest and has become one of the most economically-important rice pests in china. effective control measures are desperately needed. entomopathogenic fungi, such as beauveria bassiana (balsamo-crivelli) vuillemin (hypocreales: clavicipitaceae) and b. brongniartii (saccardo), have shown great potential for the management of some sucking pest species. in this study, to explore alternative strategies fo ... | 2014 | 25373179 |
| occurrence of fruiting structures allows determination of purpureocillium lilacinum as an inciting agent of pleuritis and pneumonia in a loggerhead sea turtle (caretta caretta) by histopathologic correlation to culture. | purpureocillium lilacinum and beauveria bassiana were isolated from lung sampled at necropsy of a 12 year-old female loggerhead sea turtle (caretta caretta) that had displayed abnormal buoyancy. histopathologic evaluation revealed pleuritis and pneumonia with non-melanized, septate hyphae and fruiting structures identical to those of p. lilacinum. this case emphasizes the importance of a histological correlate to fungal culture when environmental fungi are isolated and demonstrates the infrequen ... | 2014 | 25379399 |
| selectivity of metarhizium anisopliae and beauveria bassiana (hypocreales: clavicipitaceae) on adults of cotesia flavipes (hymenoptera: braconidae). | understanding mortality patterns and interactions between entomopathogenic fungi and parasitoids is important to improve insect biological control programs. the aim of this study was to evaluate the effect of metarhizium anisopliae (metschnikoff, 1879) sorokin, 1833 and beauveria bassiana (balsamo) vuillemin, 1912 (hypocreales: clavicipitaceae) on adults of cotesia flavipes (cameron, 1891) (hymenoptera: braconidae) with biological insecticides biometha wp plus (m. anisopliae), biovéria g (b. bas ... | 2014 | 25403080 |
| a novel mitochondrial membrane protein, ohmm, limits fungal oxidative stress resistance and virulence in the insect fungal pathogen beauveria bassiana. | the hog1 mitogen-activated protein (map) kinase regulates environmental stress responses and virulence in the entomopathogenic fungus beauveria bassiana. to further characterize this pathway, we constructed a subtraction library enriched for genes regulated by hog1. one targeted gene, encoding a novel membrane protein, ohmm (oxidative homeostasis membrane-protein-mitochondria), was uniquely identified as being downregulated in the δhog1 background during growth under non-stress and osmotic stres ... | 2015 | 25403093 |
| complete dsrna sequence of a novel victorivirus isolated from the pear stem wart fungus botryosphaeria dothidea. | botryosphaeria dothidea is a pathogen that causes many important diseases of deciduous fruit trees worldwide. in this study, a double-stranded (ds) rna of 5322 base pairs (bp) in length from strain gy25 of b. dothidea was isolated and sequenced. the dsrna potentially contained two overlapping open reading frames (orfs). the predicted gene product of orf1 had the highest amino acid sequence similarity, 37 % (e-value, 8e(-101)), to the capsid protein of rosellinia necatrix victorivirus 1 (rnv1), a ... | 2015 | 25416855 |
| rna sequencing analysis identifies the metabolic and developmental genes regulated by bbsnf1 during conidiation of the entomopathogenic fungus beauveria bassiana. | conidiation promotes fungal dispersal and survival in the environment, and is a determinant for the biocontrol potential of beauveria bassiana. the snf1/ampk protein kinases function as an important regulator of fungal development and energy metabolism, and play a crucial role in conidiation of the filamentous fungi. in previous study, it has been established that the b. bassiana homolog (bbsnf1) controls conidial production. this study showed that the δbbsnf1 mutants displayed a delayed develop ... | 2015 | 25417093 |
| production of antibacterial bombyx mori cecropin a in mealworm-pathogenic beauveria bassiana erl1170. | efforts are underway to produce antimicrobial peptides in yellow mealworms (tenebrio molitor), which can be developed as more effective and safer animal feed additives. in this work, we expressed bombyx mori (bm) cecropin-a in mealworms by the infection of transformed entomopathogenic beauveria bassiana erl1170. the active domain of bm cecropin a gene was tagged with a signal sequence of b. bassiana for extracellular secretion, and the fragment was inserted into erl1170 by the restriction enzyme ... | 2015 | 25429897 |
| mortality risk from entomopathogenic fungi affects oviposition behavior in the parasitoid wasp trybliographa rapae. | biological control of pests in agroecosystems could be enhanced by combining multiple natural enemies. however, this approach might also compromise the control efficacy through intraguild predation (igp) among the natural enemies. parasitoids may be able to avoid the risk of unidirectional igp posed by entomopathogenic fungi through selective oviposition behavior during host foraging. trybliographa rapae is a larval parasitoid of the cabbage root fly, delia radicum. here we evaluated the suscept ... | 2015 | 25446037 |
| til-type protease inhibitors may be used as targeted resistance factors to enhance silkworm defenses against invasive fungi. | entomopathogenic fungi penetrate the insect cuticle using their abundant hydrolases. these hydrolases, which include cuticle-degrading proteases and chitinases, are important virulence factors. our recent findings suggest that many serine protease inhibitors, especially til-type protease inhibitors, are involved in insect resistance to pathogenic microorganisms. to clarify the molecular mechanism underlying this resistance to entomopathogenic fungi and identify novel genes to improve the silkwor ... | 2015 | 25453359 |
| responsiveness of entomopathogenic fungi to menadione-induced oxidative stress. | entomopathogenic fungi are predisposed to ros induced by heat and uv-a radiation when outside the insect host. when inside the host, they are subject to phagocytic cells that generate ros to eliminate invading pathogens. the oxidative stress tolerance of the entomopathogenic fungi aschersonia aleyrodis (arsef 430 and 10276), aschersonia placenta (arsef 7637), beauveria bassiana (arsef 252), isaria fumosorosea (arsef 3889), lecanicillium aphanocladii (arsef 6433), metarhizium acridum (arsef 324), ... | 2014 | 25457946 |
| natural variation in virulence of the entomopathogenic fungus beauveria bassiana against malaria mosquitoes. | insecticide resistance is greatly hampering current efforts to control malaria and therefore alternative methods are needed. entomopathogenic fungi have been proposed as an alternative with a special focus on the cosmopolitan species beauveria bassiana. however, few studies have analysed the effects of natural variation within fungal isolates on mosquito survival, and the implications and possible exploitation for malaria control. | 2014 | 25480526 |
| naturally occurring entomopathogenic fungi infecting stored grain insect species in punjab, pakistan. | the occurrence of entomopathogenic fungi isolated from stored grain insect pests sampled from various geographical regions of punjab, pakistan, was investigated. in total, 25,720 insects from six different species were evaluated, and 195 isolates from 24 different fungal species were recovered. these included the ascomycetes beauveria bassiana sensu lato (balsamo) vuillemin (hypocreales: clavicipitaceae), metarhizium anisopliae sensu lato (metschnikoff) sorokin (hypocreales: clavicipitaceae), pu ... | 2014 | 25480970 |
| apolipophorin iii from honeybees (apis cerana) exhibits antibacterial activity. | apolipophorin iii (apolp-iii) is involved in lipid transport and innate immunity in insects. in this study, an apolp-iii protein that exhibits antibacterial activity was identified in honeybees (apis cerana). a. cerana apolp-iii cdna encodes a 193 amino acid sequence that shares high identity with other members of the hymenopteran insect apolp-iii family. a. cerana apolp-iii is expressed constitutively in the fat body, epidermis, and venom gland and is detected as a 23-kda protein. a. cerana apo ... | 2015 | 25483322 |
| liquid culture fermentation for rapid production of desiccation tolerant blastospores of beauveria bassiana and isaria fumosorosea strains. | a major constraint to the commercial use of fungal biocontrol agents is the availability of low-cost production media and processes. previous attempts in producing beauveria blastospores using liquid culture fermentation processes required long fermentation times (6-8days) and produced cells that had poor survival after desiccation and storage. in this study, isolates of beauveria bassiana and isaria fumosorosea were evaluated for blastospore yield, desiccation tolerance, storage stability, and ... | 2015 | 25497914 |
| laboratory bioassay of beauveria bassiana against tetranychus urticae (acari: tetranychidae) on leaf discs and potted bean plants. | use of the mycopathogen beauveria bassiana (strain gha), marketed as botanigard(®) es, was evaluated as a plant protection strategy against the spider mite tetranychus urticae koch, which is considered one of the most economically important and cosmopolitan pests of many crops. tetranychus urticae were treated with four concentrations of conidia (1 × 10(5), 1 × 10(6), 1 × 10(7), or 1 × 10(8) conidia/ml), and virulence was assessed on mites held at four relative humidity levels (35, 55, 75, and 9 ... | 2015 | 25500970 |
| improving mycoinsecticides for insect biological control. | the desire for decreased reliance on chemical pesticides continues to fuel interest in alternative means for pest control including the use of naturally occurring microbial insect pathogens. insects, as vectors of disease causing agents or as agricultural pests, are responsible for millions of deaths and significant economic losses worldwide, placing stresses on productivity (gdp) and human health and welfare. in addition, alterations in climate change are likely to affect insect ranges, expandi ... | 2015 | 25503318 |
| an insect pupal cell with antimicrobial properties that suppress an entomopathogenic fungus. | soil-dwelling insects have developed various mechanisms to defend against pathogen infection. the pecan weevil, curculio caryae, spends two to three years in the soil inside an earthen cell. we hypothesized that the cell may possess antimicrobial properties. in a laboratory study, we tested the hypothesis using the fungus beauveria bassiana as a model. b. bassiana is a common endemic pathogen of c. caryae. we compared the number of colony-forming-units on selective media when b. bassiana was exp ... | 2015 | 25510575 |
| evaluating the efficacy of biological and conventional insecticides with the new 'mcd bottle' bioassay. | control of mosquitoes requires the ability to evaluate new insecticides and to monitor resistance to existing insecticides. monitoring tools should be flexible and low cost so that they can be deployed in remote, resource poor areas. ideally, a bioassay should be able to simulate transient contact between mosquitoes and insecticides, and it should allow for excito-repellency and avoidance behaviour in mosquitoes. presented here is a new bioassay, which has been designed to meet these criteria. t ... | 2014 | 25515850 |
| alate susceptibility in ants. | pathogens are predicted to pose a particular threat to eusocial insects because infections can spread rapidly in colonies with high densities of closely related individuals. in ants, there are two major castes: workers and reproductives. sterile workers receive no direct benefit from investing in immunity, but can gain indirect fitness benefits if their immunity aids the survival of their fertile siblings. virgin reproductives (alates), on the other hand, may be able to increase their investment ... | 2014 | 25540683 |
| molecular tracing of white muscardine in the silkworm, bombyx mori (linn.) ii. silkworm white muscardine is not caused by artificial release or natural epizootic of beauveria bassiana in china. | the fungal pathogen beauveria bassiana causes serious economic losses in sericulture. its origin is usually attributed to the release of b. bassiana insecticides against pine caterpillars (dendrolimus punctuatus). in the present study, 488 b. bassiana isolates obtained from silkworm (bombyx mori) collected from 13 chinese provinces, and 327 b. bassiana isolates obtained from d. punctatus collected from 9 provinces, were analyzed for population genetic structure using the issr technique based on ... | 2015 | 25541121 |
| conidial vigor vs. viability as predictors of virulence of entomopathogenic fungi. | we tested the hypothesis that debilitated conidia exhibiting slow-germination (requiring>16h to germinate) are less virulent than vigorous conidia exhibiting fast germination (requiring⩽16h to germinate). preparations of beauveria bassiana s.l. strain cg 1027 with variable ratios of vigorous to debilitated conidia were assayed against third-instar larvae of spodoptera frugiperda. as the proportion of debilitated conidia in test preparations increased, lc50 expressed in terms of total viable coni ... | 2015 | 25573792 |
| new insights into the amphibious life of biomphalaria glabrata and susceptibility of its egg masses to fungal infection. | the air-breathing snail biomphalaria glabrata proliferates in stagnant freshwater, and nothing is known about the survival of eggs in intermittently (rather than perpetually) wet habitats. in the present study their egg masses matured, and juveniles subsequently eclosed and were mobile in a stable water film of transitory habitats simulated by two different simple test devices described here. the viability of eggs maintained in an unstable film however, was diminished. the maturation of egg mass ... | 2015 | 25576771 |
| identification and sequence determination of a novel double-stranded rna mycovirus from the entomopathogenic fungus beauveria bassiana. | an isolate of the entomopathogenic fungus beauveria bassiana was found to contain five double-stranded (ds) rna elements ranging from 1.5 to more than 3 kbp. the complete sequence of the largest dsrna element is described here. analysis of the rdrp nucleotide sequence reveals its similarity to unclassified dsrna elements, such as alternaria longipes dsrna virus 1, and its distant relationship to the rna-dependent rna polymerases of members of the family partitiviridae. | 2015 | 25577168 |
| differential metabolic responses of beauveria bassiana cultured in pupae extracts, root exudates and its interactions with insect and plant. | beauveria bassiana is a kind of world-wide entomopathogenic fungus and can also colonize plant rhizosphere. previous researches showed differential expression of genes when entomopathogenic fungi are cultured in insect or plant materials. however, so far there is no report on metabolic alterations of b. bassiana in the environments of insect or plant. the purpose of this paper is to address this problem. herein, we first provide the metabolomic analysis of b. bassiana cultured in insect pupae ex ... | 2015 | 25584432 |
| entomopathogenicity of simplicillium lanosoniveum isolated in korea. | fruiting bodies similar to those of the ascomycete fungi podostroma cornu-damae and cordyceps militaris were collected from mt. seunghak in busan, korea on august 21, 2012. the fruiting bodies were cylindrical, with tapered ends and golden red in color. the fruiting bodies contained abundant conidiophores bearing single-celled conidia, but no perithecia or asci. pure culture of the fungal isolates was obtained through single-spore isolation. analyses of morphological characteristics, including c ... | 2014 | 25606002 |
| antiangiogenic-like properties of fermented extracts of ayurvedic medicinal plants. | the three ayurvedic medicinal plants, withania somnifera, emblica officinalis, and bacopa monnieri, were extracted by high-pressure static extraction using the zippertex(®) technology. the extracts were mixed to reach quantifiable amounts of active compounds identified by high-pressure liquid chromatography-mass spectrometry (hplc-ms) analysis. the mixture of extracts was incubated with resting cells of the fungus beauveria bassiana atcc 7159. the fermentation promoted the fluidization of the st ... | 2015 | 25608021 |
| interactions between a fungal entomopathogen and malaria parasites within a mosquito vector. | mosquitoes are becoming increasingly resistant to the chemical insecticides currently available for malaria vector control, spurring interest in alternative management tools. one promising technology is the use of fungal entomopathogens. fungi have been shown to impact the potential for mosquitoes to transmit malaria by reducing mosquito longevity and altering behaviour associated with flight and host location. additionally, fungi could impact the development of malaria parasites within the mosq ... | 2015 | 25626485 |
| potential for entomopathogenic fungi to control triatoma dimidiata (hemiptera: reduviidae), a vector of chagas disease in mexico. | the use of entomopathogenic fungi to control disease vectors has become relevant because traditional chemical control methods have caused damage to the environment and led to the development of resistance among vectors. thus, this study assessed the pathogenicity of entomopathogenic fungi in triatoma dimidiata. | 2015 | 25626650 |
| subcellular localization of six thioredoxins and their antioxidant activity and contributions to biological control potential in beauveria bassiana. | thioredoxins (trx) can detoxify sulfide or act as electron donors in the reduction of disulfide and dithiol to protect yeast cells from ros damage but remain poorly explored in filamentous fungi. here we show more trx homologs in beauveria bassiana than in many other fungi and examine their functions. this filamentous entomopathogen has six trx homologs, including four (txr1-4) evidently localized in cytoplasm, one (trx5) in nuclear membrane and another (trx6) in mitochondria. deletion of each t ... | 2015 | 25636736 |
| native strains of beauveria bassiana for the control of rhipicephalus sanguineus sensu lato. | rhipicephalus sanguineus sensu lato ticks are widespread worldwide due to their adaptability to survive under different environmental conditions. they may act as vectors of a wide range of pathogens to humans and animals and their control is based on the use of chemical products on dogs and in the environment. alternative control strategies, such as the use of entomopathogenic fungi as bio-control agents have also been investigated. the ability of native strains of beauveria bassiana sensu lato ... | 2015 | 25651851 |
| biosynthesis of human diazepam and clonazepam metabolites. | a screening of fungal and microbial strains allowed to select the best microorganisms to produce in high yields some of the human metabolites of two benzodiazepine drugs, diazepam and clonazepam, in order to study new pharmacological activities and for chemical standard proposes. among the microorganisms tested, cunninghamella echinulata atcc 9244 and rhizopus arrhizus atcc 11145 strains, were the most active producers of the mains metabolites of diazepam which included demethylated, hydroxylate ... | 2015 | 25655722 |
| tenellin acts as an iron chelator to prevent iron-generated reactive oxygen species toxicity in the entomopathogenic fungus beauveria bassiana. | iron is an essential element for life. however, the iron overload can be toxic. here, we investigated the significant increase of tenellin and iron-tenellin complex production in ferricrocin-deficient mutants of beauveria bassiana. our chemical analysis indicated that the ferricrocin-deficient mutants t1, t3 and t5 nearly abolished ferricrocin production. in turn, these mutants had significant accumulation of iron-tenellin complex in their mycelia at 247-289 mg g(-1) cell dry weight under iron-r ... | 2015 | 25670702 |
| a method of multiplex pcr for detection of field released beauveria bassiana, a fungal entomopathogen applied for pest management in jute (corchorus olitorius). | the fungal entomopathogen beauveria bassiana is a promising biocontrol agent for many pests. some b. bassiana strains have been found effective against jute pests. to monitor the survival of field released b. bassiana a rapid and efficient detection technique is essential. conventional methods such as plating method or direct culture method which are based on cultivation on selective media followed by microscopy are time consuming and not so sensitive. pcr based methods are rapid, sensitive and ... | 2015 | 25680421 |
| application of two-stage cultivation for exploring the nutritional requirements for sporulation of three biocontrol fungi. | fungicide was an important part in mycopesticides, which play an important role in pest management, while their mass production and commercialization faced problem. we found that the nutrition for mycelia growth and sporulation differences a lot. so, we developed "two-step method" to define the nutrition for sporulation in this paper. the results indicated that the novel method led to a great increase of spore yields for beauveria bassiana (ibc1201), lecanicillium lecanii (ca-1-g), and pochonia ... | 2015 | 25709841 |
| genome sequence, comparative analysis, and evolutionary insights into chitinases of entomopathogenic fungus hirsutella thompsonii. | hirsutella thompsonii (ht) is a fungal pathogen of acarines and the primary cause of epizootics among mites. the draft genomes of two isolates of ht (mtcc 3556: ht3, 34.6 mb and mtcc 6686: ht6, 34.7 mb) are presented and compared with the genomes of beauveria bassiana (bb) arsef 2860 and ophiocordyceps sinensis (os) co18. comparative analysis of carbohydrate active enzymes, pathogen-host interaction genes, metabolism-associated genes, and genes involved in biosynthesis of secondary metabolites i ... | 2015 | 25716828 |
| serpin-15 from bombyx mori inhibits prophenoloxidase activation and expression of antimicrobial peptides. | serine protease inhibitors (spis) play a key role in physiological responses by controlling protease activities. in this study, we studied the biochemical functions of serpin-15, an spi, from bombyx mori (bmserpin-15). recombinant bmserpin-15 was expressed in escherichia coli cells and used to raise rabbit anti-bmserpin-15 polyclonal antibodies. bmserpin-15 mrna and protein expression was detected in all tested tissues, particularly in the fat body and silk gland. after challenge with four diffe ... | 2015 | 25720980 |
| biologically active metabolites produced by the basidiomycete quambalaria cyanescens. | four strains of the fungus quambalaria cyanescens (basidiomycota: microstromatales), were used for the determination of secondary metabolites production and their antimicrobial and biological activities. a new naphthoquinone named quambalarine a, (s)-(+)-3-(5-ethyl-tetrahydrofuran-2-yliden)-5,7,8-trihydroxy-2-oxo-1,4-naphthoquinone (1), together with two known naphthoquinones, 3-hexanoyl-2,5,7,8-tetrahydroxy-1,4-naphthoquinone (named here as quambalarine b, 2) and mompain, 2,5,7,8-tetrahydroxy-1 ... | 2015 | 25723150 |
| [insecticidal and immunosuppressive effect of ascomycete cordyceps militaris on the larvae of the colorado potato beetle leptinotarsa decemlineata]. | the immunosuppressive and insecticidal activity of cultures of the entomopathogenic fungus cordyceps militaris on the larvae of the colorado potato beetle leptinotarsa decemlineata has been established for the first time. it was found that the peroral effect of the fungal culture resulted in dose-dependent decrease in survival, delayed in development time and molting, decreases in the total hemocyt counts, increased activity of phenoloxidases in the hemolymph, and reduced activity of the enzyme ... | 2016 | 25731041 |
| molecular cloning and expression analysis of a hemolin-like molecule from antheraea pernyi. | hemolin belongs to the immunoglobulin superfamily and plays an important role in innate immune response of insects. in this study, a hemolin-like cdna of 1418bp was obtained from antheraea pernyi (ap-hemolin-like). sequence analysis revealed ap-hemolin-like was homologous to those hemolins from other insect species. recombinant ap-hemolin-like protein was expressed in escherichia coli cells, and polyclonal antibodies were produced against the recombinant proteins. real-time pcr and western blot ... | 2015 | 25812765 |
| variations in oxygen concentration cause differential antioxidant response and expression of related genes in beauveria bassiana. | the entomopathogenic fungus beauveria bassiana is widely used in pest biocontrol strategies. we evaluated both the antioxidant response mediated by compatible solutes, trehalose or mannitol, and the expression of related genes using oxygen pulses at three oxygen concentrations in solid state culture (ssc): normal atmosphere (21% o2), low oxygen (16% o2) and enriched oxygen (26% o2). trehalose concentration decreased 75% after atmospheric modifications in the cultures, whereas mannitol synthesis ... | 2015 | 25813512 |
| influence of different types of phyllostachys pubescens (poales: poaceae) leaves on population parameters of pantana phyllostachysae (lepidoptera: lymantriidae) and parasitic effects of beauveria bassiana (moniliales: moniliaceae). | we found that pantana phyllostachysae, a dangerous pest of moso bamboo (phyllostachys pubescens), showed differences in growth and development after feeding on diverse types of moso bamboo leaves. the mortality rate of pa. phyllostachysae due to beauveria bassiana, an entomopathogenic fungus, was also affected by the varied larval diet. larval and pupal developmental duration of pa. phyllostachysae was longer when feeding on "off-year" bamboo leaves. pupal weight and adult fertility were higher ... | 2015 | 25843592 |
| inhibitors of eicosanoid biosynthesis influencing the transcripts level of shsp21.4 gene induced by pathogen infections, in antheraea pernyi. | small heat shock proteins (shsps) can regulate protein folding and protect cells from stress. to investigate the role of shsps in the silk-producing insect antheraea pernyi response to microorganisms, a shsp gene termed as ap-shsp21.4, was identified. this gene encoded a 21.4 kda protein which shares the conserved structure of insect shsps and belongs to shsp21.4 family. ap-shsp21.4 was highly expressed in fat body and up-regulated in midgut and fat body of a. pernyi challenged with escherichia ... | 2015 | 25844646 |
| molecular characterization of a novel amalgavirus from the entomopathogenic fungus beauveria bassiana. | beauveria bassiana is a ubiquitous entomopathogen infecting hundreds of insect species. we have determined the genomic organization and the complete nucleotide sequence of a novel virus isolated from the isolate a24 of b. bassiana. phylogenetic analysis of the polymerase gene reveals that the virus, tentatively named beauveria bassiana virus 1, belongs to the family amalgaviridae and represents a distinct lineage of amalgaviruses infecting fungi. | 2015 | 25854690 |
| potential of two entomopathogenic fungi, beauveria bassiana and metarhizium anisopliae (coleoptera: scarabaeidae), as biological control agents against the june beetle. | the aim of this study was to evaluate the effectiveness of the entomopathogenic fungi (epf), beauveria bassiana (bals.) vuill. (deuteromycotina: hyphomycetes) strain ppri 5339 [broadband, an emulsifiable spore concentrate (ec) formulation] and metarhizium anisopliae (metsch.) sorokin (hypocreales: clavicipitaceae) strain f52 [met52, both ec and granular (gr) formulations] against the larvae of polyphylla fullo (l.) (coleoptera: scarabaeidae). larvicidal bioassays were performed in foam boxes (10 ... | 2015 | 25881632 |
| metabolomics reveals insect metabolic responses associated with fungal infection. | the interactions between insects and pathogenic fungi are complex. we employed metabolomic techniques to profile insect metabolic dynamics upon infection by the pathogenic fungus beauveria bassiana. silkworm larvae were infected with fungal spores and microscopic observations demonstrated that the exhaustion of insect hemocytes was coupled with fungal propagation in the insect body cavity. metabolomic analyses revealed that fungal infection could significantly alter insect energy and nutrient me ... | 2015 | 25895944 |
| [species diversity and temporal niche of entomopathogenic fungi in the extensively managed tea plantation soil]. | the species diversity and temporal niche of entomopathogenic fungi community in the rhizosphere soil collected from the extensively managed huangshan fuzz tip tea plantation were investigated. a total of 140 soil samples were collected at the location of tangkou town, huangshan of anhui province during august, 2012 to june, 2013, and totally 1041 fungal isolates were obtained on selective medium with soil dilution plating. the results showed that the entomopathogenic fungi community in the tea p ... | 2014 | 25898633 |