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antioxidant effect of azadirachta indica on high fat diet induced diabetic charles foster rats.oxidative stress plays a major role in the pathogenesis of both types of diabetes mellitus. excessively high levels of free radicals cause damage to cellular proteins, membrane lipids and nucleic acids, and eventually cell death. the present study was designed to investigate the possible effect of azadirachta indica leaf extract in high fat diet induced diabetic charles foster rats. the increased level of lipidperoxidation and altered levels of enzymatic (superoxide dismutase, glutathione peroxi ...201222544689
hypoglycemic effect or neem bark and flower on streptozotocin - induced diabetes in mice.three to six hours after the administration of extracts of neem bark or flower, the blood glucose levels of normal and streptozotocin-induced diabetic mice dropped significantly. however, neem flower possesses stronger hypoglycemic activity than the bark. hypoglycemic action of these extracts may be due to their metabolic effect on tissue and or due to increase in insulin secretion.199122556555
effect of azadirachta indica leaf extract on oestrous cycle of rats.effect of azadirachta indica leaf extracts on duration of oestrous cycle of adult rats was studied. the extract (200 mg/kg) induced cornified phase of the cycle which persisted till the last day of treatment. possible reasons behind the results are discussed.199322556624
protective effect of fraction of azadirachta indica leaf extract on carbon tetrachloride induced hepatotoxicity.the hepatoprotective activity of a fraction of the leaf extract of a.indica against carbon tetrachloride : liquid paraffin (1:1) induced liver damage in rats at doses of 100 mg/kg and 200 mg/kg was evaluated. a significant dose dependent hepatoprotective activity was evidenced by lowering of the elevated levels of glutamate oxaloacetate transaminase (got), glutamate pyruvate transaminase (gpt), acid phosphatase (acp) and alkaline phosphatase (alp) in the serum of ccl(4) : liquid paraffin (1:1) t ...199422556679
anti-cholesterolaemic activity of a fraction of azadirachta indica leaf extract on rats.a fraction of leaf extract isolated from leaves of azadirachta indica (meliaceae) was tested for lowering of blood cholesterol level in rats. the extract at a dose of 200 mg/kg i.p. significantly lowered the blood cholesterol level in test animals.199522556692
multicentric, placebo-controlled, randomised double-blind evaluation of a new herbal cream in vagial infections.efficacyand safety of a new herbal cream containing aqueous extracts of azadirachta indica, curcuma longa, pongamia glabra, glycyrrihiza glabra and santallum album were evaluated in amulticentric, randomized, double-blind, placebo-controlled study. with active drug treatment, there was significant improvement in various signs like redness, oedema and symptoms like itching, burning, discharge and discomfort, compared to placebo treatment. microscopic examination of smear and culture showed signif ...199522556701
chromatographic studies on a herb decoction.argwadhadhi kwatham is a compound preparation of 3 single drugs viz cassia fistula l. azadirachta indica a., tinospora cordifolia l. literature survey show tat all3 ingredients are very effective for skin diseases. kahayams prepared individually with these 3 drugs was found to be more effective for skin dideases. this paper deals with a comparative study of the chemical constituents present in aragwadhadhi kwatham and the individual kwathams, all the four kwarham were chemically analysed t.l.c. ...199822556839
anti - inflammatory and antimicrobial activities of the root, bark and leaves of azadirachta indica.azadirachta indica is a plant of varied uses in ayurveda since ancient times and is highly extolled by expert physicians and as well as practitioners of folk medicines. almost every part of the tree has long been used in folklore and traditional systems of medicine for the treatment of a variety of human ailments. the 50% acetone extract of the root, bark and leaves of a. indica sowed marked anti- inflammatory activity in carrageenan induced edema in rats, antimicrobial activity was also tested.199822556865
on the antifeedant and anthellmintic potential of anacardium occidentale l.the antifeedant and anthelmintic activities of crude extracts of the shells of anacardium occidentale were studied by comparing it's activity with the activity of azadirachta indica a juss (kernel), a commercial standard. the test extracts of a. occidentale in petroleum ether, dichloromethane: methanol showed better activities compared to a. indica. to study the anthelmintic activity, the addition of piperine to the formulation significantly enhances the activity. the phytochemical investigation ...199822556881
antifertility efficacy of neem bark (azadirachta indica a.juss.) in male rats.subcutaneous treatment of 50% etoh extract of neem bark (azadirachta indica a. juss) to male rats at the dose of 10 mg for 30 days caused arrest of spermatogenesis. the diameter of serminiferous tubule, leydig cell nucleus and epithelial heights of epididymides were significantly reduced (p≤0.001). sperm motility and density were significantly reduced. reduced testicular and accessory organ protein, sialic acid and vesicular fructose content manifested arrest of spermatogenesis and depletion of ...199922556912
preparation and evaluation of tincture of azadirachta indica a. juss. leaves.different strength of alcohol 40.50,60,70,80,90 per cent (v/v) and absolute were utilized for the preparation of tincture of azadirachta indica a. juss leaves by maceration for 7, 14,21 days. 60 per cent tincture produced after 21 says of maceration showed a maximum solid content of 1.425 per cent (v/v) and sowed ph 3.15. this tincture was characterized by studying is various physico-chmicla properties along wit its thin-layer chromatographic characteristics.199922556923
pharmacognostic profiles of azadirachta indica a.juss.leaves.the microscopic and macroscopic characters of the leaves, physical constant values, behavior on treatment with different chemical reagents, fluorescence characters under ultra violet light after treatment with different chemical reagents of the powdered leaves of azadirachta indica a. juss (family-meliaceae) were studies to fix some pharmacognostical parameters.200022556932
spermicidal activity in vitro of bark extract of azadirachta indica in rats.ethanolic extract of bark of azadirachta indica showed potent spermicidal activity when tested on rat spermatozoa in vitro. when the extract was applied in 1:1 and 1:2 ratio at tree different concentration using wet drop method, cent percent mortality of spermatozoa was observed within 10 seconds. 10% concentration of the extract was considered as an effective dose.200022557001
preparation and evaluation of herbal shampoo powder.two preparations of herbal shampoo powder were formulated using some common traditional drugs used by folk and traditional people of bundelkhand region (m.p) india, for hair care. the preparations were formulated using bahera, amla, neem tulsi, shikakai henna & brahmi evaluated for organoleptic, powder charecterestics, foam test and physical evaluation. as the selected drugs being used since long time as single drug or in combination, present investigations will further help to establish a stand ...200422557149
a comparative study on the effect of plant extracts with the antibiotics on organisms of hospital origin.thirty five plants belonging to twenty families were studied for their antimicrobial activity. among the plants tested, 43 % showed antimicrobial activity. fifteen plants belonging to 10 families exhibited activity against gram positive bacteria and gram negative bacteria. four plants namely azadirachta indica, garadenia jasminoides, magnifera indica, and wrightia tinctora showed an appreciable activity against the gram positive bacteria and seven plants against gram negative organisms. leaf ext ...200622557227
some pharmacological actions of 'nimbidin'- a bitter principle of azadirachta indica-a juss (neem).nimbidin the major bitter principle from oil of seeds of azadirachta indica a. juss was investigated for various pharmacological actions in a number animal models. on central nervous system it exhibited moderate sedative effect it did not show any significant cardiovascular effects in experimental animals. autonomic pharmacological studies (in-vitro) revealed pronounced anticholinergic antihistaminic (h1-receptor), anti 5 ht and antinicotinic activities. but in-vivo tests did not show any antich ...198422557456
effect of nimbidin on gastric acid secretion.nimbidin, the crude bitter compound from neem has been investigated for its gastric anti secretory activity in rats and cats. it exhibited significant anti - secretory activity in pylorus ligated rats and cats. in lumen - perfused rats it suppressed the basal as well as histamine and carbachol stimulated gastric acid output at 40 mg/kg (i.v). however it had no effect on asa - induced back - diffusion of h+ ions. this anti secretory activity of nimbidin was found to be similar to that of h2 - rec ...198522557506
'antiandrogenic properties of neem seed oil (azadirachta indica) in male rat and rabbi'.azadirachta indica (neem) seed oil brings about a significant diminution in spermatozoan motility and density. it leads to reduction of fertility rate in rats and rabbits. the body weight of the animals remain unaffected but the weights of reproductive oranges declined. reduction of cauda epididymal protein, sialic acid, acid phosphatase and seminal vesicular fructose concentration in rats and rabbits could bedue to antiandrogenic action of the seed oil as confirmed with the help of bioassay tec ...198722557585
anti-plasmodial effects of azadirachta indica in experimental cerebral malaria: apoptosis of cerebellar purkinje cells of mice as a marker.malaria is a major public health problem in the world, but treatment of malaria is becoming more difficult due to increasing drug resistance. therefore, the need for alternative drugs is acute.201022558559
azadirachtin production by hairy root cultivation of azadirachta indica in a modified stirred tank reactor.present investigation involves hairy root cultivation of azadirachta indica in a modified stirred tank reactor under optimized culture conditions for maximum volumetric productivity of azadirachtin. the selected hairy root line (az-35) was induced via agrobacterium rhizogenes lba 920-mediated transformation of a. indica leaf explants (coimbatore variety, india). liquid culture of the hairy roots was developed in a modified murashige and skoog medium (mm2). to further enhance the productivity of ...201222580745
post-surgical scalp wounds with exposed bone treated with a plant-derived wound therapeutic.to evaluate the efficacy of a plant-derived wound dressing, a mixture of hypericum oil (hypericum perforatum) and neem oil (azadirachta indica), in scalp wounds with exposed bone.201222584740
histopathological, enzymatic, and molecular alterations induced by cigarette smoke inhalation in the pulmonary tissue of mice and its amelioration by aqueous azadirachta indica leaf extract.the modulatory and protective effects of aqueous azadirachta indica leaf extract (aaile) had been investigated against cigarette smoke-induced pulmonary oxidative stress and risk of lung cancer. male balb/c mice were divided in 4 groups: control, cigarette smoke (cs), aaile, and aaile + cs. animals in the cs group were exposed to cigarette smoke for a period of 10 weeks. aaile oral administration at a dosage of 100 mg/kg body weight was started 2 weeks prior to cs exposure and continued until th ...201222591280
an ethnobotanical survey of medicinal plants used in terai forest of western nepal.nepal himalayas have been known as a rich source for valuable medicinal plants since vedic periods. present work is the documentation of indigenous knowledge on plant utilization as natural remedy by the inhabitants of terai forest in western nepal.201222591592
mapping of fluoride endemic area and assessment of f(-1) accumulation in soil and vegetation.the prevalence of fluorosis is mainly due to the consumption of more fluoride (f(-1)) through drinking water, vegetables, and crops. the objective of the study was mapping of f(-1) endemic area of newai tehsil, tonk district, rajasthan, india. for the present study, water, soil (0-45 cm), and vegetation samples were collected from 17 villages. fluoride concentration in water samples ranged from 0.3 to 9.8 mg/l. out of 17 villages studied, the amounts of f(-1) content of eight villages were found ...201322638723
response surface modelling of cr6+ adsorption from aqueous solution by neem bark powder: box-behnken experimental approach.the main aim of this study is to investigate the combined effect of different operating parameters like adsorbent dose, initial cr(6+) concentration and ph on the removal of cr(6+) from aqueous solution using neem bark powder (nbp). a series of batch experiments were performed to find out the adsorption isotherms and kinetic behaviour of cr(6+) in the aqueous solution. the adsorption process was examined with three independent variables viz. nbp dosage, initial cr(6+) concentrations and ph. seve ...201322645009
novel anti-inflammatory activity of epoxyazadiradione against macrophage migration inhibitory factor: inhibition of tautomerase and proinflammatory activities of macrophage migration inhibitory factor.macrophage migration inhibitory factor (mif) is responsible for proinflammatory reactions in various infectious and non-infectious diseases. we have investigated the mechanism of anti-inflammatory activity of epoxyazadiradione, a limonoid purified from neem (azadirachta indica) fruits, against mif. epoxyazadiradione inhibited the tautomerase activity of mif of both human (humif) and malaria parasites (plasmodium falciparum (pfmif) and plasmodium yoelii (pymif)) non-competitively in a reversible ...201222645149
a comparative study of vamana and virechana karma in the management of sthula pramehi w.s.r. to type-2 diabetes.in the present study, vamana and virechana along with palliative treatment was given to patients of sthula prameha (diabetes mellitus or dm). an attempt was made to compare and evaluate the role of shodhana therapy (vamana and virechana). total 47 patients of sthula pramehi were registered in this study, out of which 38 completed the treatment. the patients were divided into two groups, viz. vamana with shamana (group a) and virechana with shamana (group b). eighteen patients completed the treat ...201122661850
botanicals to control soft rot bacteria of potato.extracts from eleven different plant species such as jute (corchorus capsularis l.), cheerota (swertia chiraita ham.), chatim (alstonia scholaris l.), mander (erythrina variegata), bael (aegle marmelos l.), marigold (tagetes erecta), onion (allium cepa), garlic (allium sativum l.), neem (azadiracta indica), lime (citrus aurantifolia), and turmeric (curcuma longa l.) were tested for antibacterial activity against potato soft rot bacteria, e. carotovora subsp. carotovora (ecc) p-138, under in vitr ...201222701096
inhibitory effects of essential oils of medicinal plants from growth of plant pathogenic fungi.plant cells produce a vast amount of secondary metabolites. production of some compounds is restricted to a single species. some compounds are nearly always found only in certain specific plant organs and during a specific developmental period of the plant. some secondary metabolites of plants serve as defensive compounds against invading microorganisms. nowadays, it is attempted to substitute the biological and natural agents with chemically synthesized fungicides. in the present research, the ...201122702190
a study on antioxidant and apoptotic effect of azadirachta indica (neem) in cases of cervical cancer.to study the anti-oxidant properties of neem (azadirachta indica) in inducing apoptosis of cervical cancer cells and estimation of caspase activity and tnf-α and ifn-γ levels in monocytes of cervical cancer patients and controls.201222718100
effects of neem oil (azadirachta indica a. juss) on midgut cells of predatory larvae ceraeochrysa claveri (navás, 1911) (neuroptera: chrysopidae).the effects of ingested neem oil, a botanical insecticide obtained from the seeds of the neem tree, azadirachta indica, on the midgut cells of predatory larvae ceraeochrysa claveri were analyzed. c. claveri were fed on eggs of diatraea saccharalis treated with neem oil at a concentration of 0.5%, 1% and 2% during throughout the larval period. light and electron microscopy showed severe damages in columnar cells, which had many cytoplasmic protrusions, clustering and ruptured of the microvilli, s ...201322739123
quality assessment and scavenging activity of siamese neem flower extract.young leaves and flowers of siamese neem tree (azadirachta indica a. juss. var. siamensis valeton) are commonly consumed as a bitter tonic vegetable. active antioxidant components in the flowers are rutin and quercetin flavonoids. the aqueous extracts of young flowers collected from 14 different locations in thailand were quantitatively analysed using high-performance liquid chromatography for the contents of rutin and quercetin, and were determined for the loss on drying, heavy metals and pesti ...201322788542
control of root-knot nematodes on tomato in stone wool substrate with biological nematicides.the efficacy of four biological nematicides on root-galling, root-knot nematode (meloidogyne incognita) reproduction, and shoot weight of tomato (solanum lycopersicum) grown in stone wool substrate or in pots with sandy soil was compared to an oxamyl treatment and a non-treated control. in stone wool grown tomato, avid® (a.i. abamectin) was highly effective when applied as a drench at time of nematode inoculation. it strongly reduced root-galling and nematode reproduction, and prevented a reduct ...201122791920
characterization of nimbidiol as a potent intestinal disaccharidase and glucoamylase inhibitor present in azadirachta indica (neem) useful for the treatment of diabetes.azadirachta indica, used in antidiabetic herbal drugs, was reported to contain α-glucosidase inhibitor. bioassay guided purification characterized the inhibitor as nimbidiol (a diterpenoid), present in root and stem-bark of the tree. nimbidiol inhibited intestinal (mammalian) maltase-glucoamylase, sucrase-isomaltase, lactase, trehalase and fungal α-glucosidases. nimbidiol showed a mixed competitive inhibition on intestinal carbohydrases. ic50, ki and ki' (µm) were 1.35 ± 0.12, 0.08 ± 0.01, 0.25 ...201322803678
an endophytic fungus from azadirachta indica a. juss. that produces azadirachtin.azadirachtin a and its structural analogues are a well-known class of natural insecticides having antifeedant and insect growth-regulating properties. these compounds are exclusive to the neem tree, azadirachta indica a. juss, from where they are currently sourced. here we report for the first time, the isolation and characterization of a novel endophytic fungus from a. indica, which produces azadirachtin a and b in rich mycological medium (sabouraud dextrose broth), under shake-flask fermentati ...201222805849
diversity of plant oil seed-associated fungi isolated from seven oil-bearing seeds and their potential for the production of lipolytic enzymes.commercial oil-yielding seeds (castor, coconut, neem, peanut, pongamia, rubber and sesame) were collected from different places in the state of tamil nadu (india) from which 1279 endophytic fungi were isolated. the oil-bearing seeds exhibited rich fungal diversity. high shannon-index h' was observed with pongamia seeds (2.847) while a low index occurred for coconut kernel-associated mycoflora (1.018). maximum colonization frequency (%) was observed for lasiodiplodia theobromae (176). dominance i ...201122806781
efficacy of natural products against callosobruchus chinensis (coleoptera: bruchidae) in nepal.this study was conducted to determine the efficacy of local natural products against the beetle, callosobruchus chinensis l. (coleoptera: bruchidae), in stored chickpea cicer arietinum l. (fabaceae) in chitwan, nepal. five natural products and one synthetic pesticide (malathion) and two storage regimes (aluminum sheet bin vs. jute bags with plastic lining) were tested for their effect on stored pulse with respect to damage by c. chinensis. the five natural products included xanthoylum armatum dc ...201222812152
the in vitro antiviral property of azadirachta indica polysaccharides for poliovirus.azadirachta indica a. juss, popularly known as neem, has been extensively used in ayurvedic medicine by indian population for over 2000 years. it is used traditionally for the healing of various diseases. natural products and their derivatives provide an excellent source for new anti-viral drugs.201222855945
antioxidant enzyme changes in neem, pigeonpea and mulberry leaves in two stages of maturity.differential expression of antioxidant enzymes in various growth and differentiation stages has been documented in several plant species. we studied here, the difference in the levels of protein content and antioxidant enzymes activity at two stages of maturity, named young and mature in neem (azadirachta indica a. juss), pigeonpea (cajanus cajan (l.) mill sp) and mulberry (morus alba l.) leaves. the results showed that detached neem and pigeonpea mature leaves possessed higher activities of cat ...201222895104
neem oil limonoids induces p53-independent apoptosis and autophagy.azadirachta indica, commonly known as neem, has a wide range of medicinal properties. neem extracts and its purified products have been examined for induction of apoptosis in multiple cancer cell types; however, its underlying mechanisms remain undefined. we show that neem oil (i.e., neem), which contains majority of neem limonoids including azadirachtin, induced apoptotic and autophagic cell death. gene silencing demonstrated that caspase cascade was initiated by the activation of caspase-9, wh ...201222915764
treatment with azadirachta indica in diabetic pregnant rats: negative effects on maternal outcome.the role of azadirachta indica (neem) against chagas disease and its antibiotic and antidiabetic action have been demonstrated in non-pregnant animals. however, the effects of neem on lipid metabolism and oxidative stress during pregnancy remain to be investigated. the objective of this study was to evaluate the effects of azadirachta indica (neem) on maternal reproductive performance and biochemical parameters in non-diabetic and streptozotocin-induced mild diabetic rats (md).201222921950
insecticide susceptibility status in three medically important species of mosquitoes, anopheles stephensi, aedes aegypti and culex quinquefasciatus, from bruhat bengaluru mahanagara palike, karnataka, india.development of resistance among mosquito vectors of diseases is of growing concern in many countries of the subtropics, including much of india. the distributions of susceptibility/resistance to insecticides are scarcely recorded and are unknown in many parts of india. this manuscript reports the susceptibility status among strains of anopheles stephensi, aedes aegypti and culex quinquefasciatus collected from different geographical areas of bruhat bengaluru mahanagara palike (bbmp), karnataka, ...201322926921
anti-nociceptive and anti-inflammatory activities of azadirachta indica fruit skin extract and its isolated constituent azadiradione.this study was carried out to evaluate the anti-nociceptive and anti-inflammatory activities of carbon tetrachloride extract (ctce) of azadirachta indica fruit skin and its isolated constituent azadiradione at two different dose levels (50 and 100 mg kg⁻¹ body weight). anti-nociceptive screening by writhing test and hot-plate technique supported both peripheral and central mechanisms, respectively. anti-inflammatory activity was observed using carrageenan-induced paw oedema model. the results co ...201322928548
investigation of the chemopreventive potential of neem leaf subfractions in the hamster buccal pouch model and phytochemical characterization.chemoprevention by medicinal plants has evolved as a practical strategy to control the incidence of cancer. azadirachta indica (neem) containing various bioactive components is a promising candidate for chemoprevention. the present study was undertaken to evaluate the chemopreventive efficacy of the bioactive subfractions ethyl acetate chloroform insoluble fraction (ecif) and the methanol ethyl acetate insoluble fraction (meif) following activity-guided fractionation of neem leaf extract. analys ...201222939101
biodegradable polymer based encapsulation of neem oil nanoemulsion for controlled release of aza-a.azadirachtin a biological compound found in neem have medicinal and pesticidal properties. the present work reports on the encapsulation of neem oil nanoemulsion using sodium alginate (na-alg) by cross linking with glutaraldehyde. starch and polyethylene glycol (peg) were used as coating agents for smooth surface of beads. the sem images showed beads exhibited nearly spherical shape. swelling of the polymeric beads reduced with coating which in turn decreased the rate of release of aza-a. starch ...201222944443
immunological and physiological parameters of biomphalaria alexandrina snails exposed to azadirachta indica plant.plant molluscicides could be appropriate for snail control measures against schistosomiasis in endemic areas.201222957428
allergic contact dermatitis caused by neem oil. 201222957489
a draft of the genome and four transcriptomes of a medicinal and pesticidal angiosperm azadirachta indica.the azadirachta indica (neem) tree is a source of a wide number of natural products, including the potent biopesticide azadirachtin. in spite of its widespread applications in agriculture and medicine, the molecular aspects of the biosynthesis of neem terpenoids remain largely unexplored. the current report describes the draft genome and four transcriptomes of a. indica and attempts to contextualise the sequence information in terms of its molecular phylogeny, transcript expression and terpenoid ...201222958331
sensitivity of two in vitro assays for evaluating plant activity against the infective stage of haemonchus contortus strains.the sensitivity of larval paralysis assay (lpa) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide-formazan (mtt-formazan) assay was compared to evaluate the anthelmintic activity of plant extracts. in this study, the methanolic extract of azadirachta indica (neem) was evaluated for its activity against the infective-stage larvae (l(3)) of susceptible and resistant haemonchus contortus strains using the two aforementioned assays. in both in vitro assays, the same serial concentrat ...201322961237
protective effect of azadirachta indica extract against eimeria papillata-induced coccidiosis.coccidiosis in poultry is caused by protozoan parasites of the genus eimeria, which is responsible for worldwide economic losses. the methanolic extract of azadirachta indica (neem) leaves was used in vivo for its pharmacological, antioxidant, and anticoccidial properties. four groups of mice were investigated. the first group was inoculated only with sterile saline and served as the control group. the second group was treated by oral gavage with neem extract (500 mg/kg) daily for 4 days. the th ...201322972359
meliacinolin: a potent α-glucosidase and α-amylase inhibitor isolated from azadirachta indica leaves and in vivo antidiabetic property in streptozotocin-nicotinamide-induced type 2 diabetes in mice.in india, azadirachta indica is typically known as 'neem tree' and its leaves has long been used in the ayurvedic medical tradition as a treatment for diabetes mellitus. in-depth chromatographic investigation on chloroform extract resulted in identification of one new tetranortriterpenoid. structural elucidation was established on the basis of spectral data as 24,25,26,27-tetranor-apotirucalla-(apoeupha)-1α-senecioyloxy-3α,7α-dihydroxy-14,20,22-trien-21,23-epoxy named by us as meliacinolin (1). ...201222975503
biotransformation of oral contraceptive ethynodiol diacetate with microbial and plant cell cultures. 201223021311
endophytic fungal flora from roots and fruits of an indian neem plant azadirachta indica a. juss., and impact of culture media on their isolation.azadirachta indica a. juss. (neem), native to india, is well known worldwide for its insecticidal and ethanopharmacological properties. although endophytic microbes are known from this plant as only leaves and stems were the subjects of past reports. now, a variety of procedures and a number of different media were used to isolate the maximum number of endophytic fungi from unripe fruits and roots. a total of 272 isolates of 29 filamentous fungal taxa were isolated at rate of 68.0% from 400 samp ...201123024409
artificial neural network and multiple regression model for nickel(ii) adsorption on powdered activated carbons.nickel removal efficiency of powered activated carbons of coconut oilcake, neem oilcake and commercial carbon was investigated by using artificial neural network. the effective parameters for the removal of nickel (%r) by adsorption process, which included the ph, contact time (t), distinctiveness of activated carbon (cn), amount of activated carbon (cw) and initial concentration of nickel (co) were investigated. levenberg-marquardt (lm) back-propagation algorithm is used to train the network. t ...201123029923
azadirachta indica acts as a pro-oxidant and modulates cell cycle associated proteins during dmba/tpa induced skin carcinogenesis in mice.the present study was designed to determine the modulatory effect of aqueous azadirachta indica leaf extract (aaile) on cell cycle-associated proteins during two-stage skin carcinogenesis in mice. considering the dual role of reactive oxygen species in cancer and its chemoprevention, the levels of lipid peroxidation (index of peroxidative damage) were also determined. skin tumours were induced by topical application of 7,12-dimethylbenz(a)anthracene (dmba) as a carcinogen followed by the repetit ...201323055378
ethnopharmacological survey of medicinal plants used by indigenous and tribal people in rangamati, bangladesh.there is very limited information regarding plants used by traditional healers in rangamati, bangladesh, for treating general ailments. current study provides significant ethnopharmacological information, both qualitative and quantitative on medical plants in rangamati.201223064284
evaluation of anti-plaque microbial activity of azadirachta indica (neem oil) in vitro: a pilot study.probably microbial plaque is the main etiology for periodontal tissue inflammation. various chemical agents have been evaluated over the years with respect to their antimicrobial effects in the oral cavity. however, all are associated with side effects that prohibit regular long-term use. therefore, the effectiveness of azadirachta indica (neem) against plaque formation is considered to be vital, with lesser side effects. the aim of the present study is to evaluate and prove the antimicrobial ac ...201223066297
medicinal plants potential and use by pastoral and agro-pastoral communities in erer valley of babile wereda, eastern ethiopia.ethiopian plants have shown remarkably effective medicinal values for many human and livestock ailments. some research results are found on medicinal plants of the south, south west, central, north and north western parts of ethiopia. however, there is lack of data that quantitatively assesses the resource potential and the indigenous knowledge on use and management of medicinal plants in eastern ethiopia. the main thrust of the present ethnobotanical study centres around the potential and use o ...201223082858
ethnobotanical survey for potential anti-malarial plants in south-western nigeria.the ethnobotanical study surveyed the different types of medicinal plants used for the treatment of malaria in the south-western region of nigeria.201223085021
rhipicephalus sanguineus (acari: ixodidae) female ticks exposed to castor oil (ricinus communis): an ultrastructural overview.tick control has been accomplished through the use of synthetic acaricides, which has created resistant individuals, as well as contaminating the environment and nontarget organisms. substances of plant origin, such as oils and extracts of eucalyptus and neem leaves, have been researched as an alternative to replace the synthetic acaricides. ricinoleic acid esters from castor oil have recently been shown as a promising alternative in eliminating bacterial contamination during ethanol fermentatio ...201323086445
induction of apoptosis in human breast cancer cells by nimbolide through extrinsic and intrinsic pathway.we aimed to investigate the cytotoxic effects of nimbolide, a limonoid present in leaves and flowers of the neem tree (azadirachta indica) on human breast cancer cells. the molecular mechanisms involved in the apoptotic activity exerted by nimbolide were studied on the estrogen dependent (mcf-7) and estrogen independent (mda-mb-231) human breast cancer cell lines. the growth inhibitory effect of nimbolide was assessed by mtt assay. apoptosis induction by nimbolide treatment was determined by jc- ...201223089555
antiviral activity and mode of action of extracts from neem seed kernel against duck plague virus in vitro1.four fractions obtained from alcohol extracts of neem (azadirachta indica) seed kernel by column chromatography were investigated for antivirus activity against the duck plague virus (dpv) in vitro. duck embryo fibroblasts (def) infected with dpv were treated with the neem seed kernel extracts, and the effect of antivirus was judged by 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di-phenytetrazoliumromide colorimetric method assay and direct immunofluorescence assay. the mode of action was tested by th ...201223091135
the cytotoxic and antileishmanial activity of extracts and fractions of leaves and fruits of azadirachta indica (a juss.).the leishmaniases are severe parasitic diseases that occur worldwide, caused by protozoa of the genus leishmania. studies with medicinal plants can lead to a range of possibilities for treating and improving the patients' quality of life. research on azadirachta indica fractions and extracts has shown that they have excellent anti-leishmanial activity based on bioactivity-guided fractionation of ethanolic extracts of leaves and seeds and in vitro activity against promastigotes. in this research ...201223096354
reversal of diabetic retinopathy in streptozotocin induced diabetic rats using traditional indian anti-diabetic plant,azadirachta indica (l.).this study was carried out to investigate the effect of oral feeding of water extract of fresh leaves ofazadirachta indica (fam:meliaceae) in streptozotocin induced diabetes and its associated retinopathy in rats. treatment of the diabetic rats with aqueous extract of leaves ofa. indica at a dose of 250 mg/kg body weight for 16 weeks resulted in gradual but significant fall in blood glucose and improvement in serum total, ldl and hdl cholesterol and triacylglycerol which increased in diabetic ra ...200223105362
effect of herbal hypoglycemic agents on oxidative stress and antioxidant status in diabetic rats.in the present study the antioxidative potential ofmomordica charantia, azadirachta indica, allium sativum andocimum sanctum was assessed in streptozotocin induced diabetic rats. lipid peroxide levels were also measured in normal, diabetic and treated animals. malondialdehyde (mda) levels were significantly higher and antioxidant activity was found low in diabetic groups as compared to the control groups, and significant alteration in both the mda levels and antioxidant activity was also observe ...200323105386
tissue engineered plant extracts as nanofibrous wound dressing.use of plant extracts for treatment of burns and wound is a common practice followed over the decades and it is an important aspect of health management. many medicinal plants have a long history of curative properties in wound healing. electrospun nanofibers provide high porosity with large surface area-to-volume ratio and are more appropriate for cell accommodation, nutrition infiltration, gas exchange and waste excretion. electrospinning makes it possible to combine the advantages of utilizin ...201323111334
strengthening of antioxidant defense by azadirachta indica in alloxan-diabetic rat tissues.azadirachta indica has been reported to correct altered glycaemia in diabetes.201223125509
in-vivo and in-vitro activities of medicinal plants on ecto, endo and haemoparasitic infections: a review.crude methanol, pet. ether, ethyl acetate, n-hexane, dichloromethane and aqueous extracts of various species of medicinal plants have shown significant in-vivo and in-vitro pharmacological activities against ecto, endo and haemoparasites. the scientific evaluations of the use of the plants as antiparasitic agents were based on the claims of folklore drawn from traditional healers from various communities across the world. the pharmacological activities of these plants were associated with the pr ...201423173971
azadirachta indica (neem) leaf dietary effects on the immunity response and disease resistance of asian seabass, lates calcarifer challenged with vibrio harveyi.the present study was aimed to address the possible evaluation of azadirachta indica (neem) leaf-supplemented diets on innate immune response in asian seabass, lates calcarifer fingerlings against vibrio harveyi infection. fish were fed for two weeks diets containing six graded levels of neem leaf at 0 g, 1 g, 2 g, 3 g, 4 g and 5 g per kg feed. fish fed neem leaf-supplemented diet displayed significant differences (p < 0.05) in weight gain, specific growth rate (sgr) and feed conversion ratio (f ...201323178500
neem leaf glycoprotein is nontoxic to physiological functions of swiss mice and sprague dawley rats: histological, biochemical and immunological perspectives.we have evaluated the toxicity profile of a unique immunomodulator, neem leaf glycoprotein (nlgp) on different physiological systems of swiss mice and sprague dawley rats. nlgp injection, even in higher doses than effective concentration caused no behavioral changes in animals and no death. nlgp injection increased the body weights of mice slightly without any change in organ weights. nlgp showed no adverse effect on the hematological system. moreover, little hematostimulation was noticed, as ev ...201323178577
azadirachta indica mitigates behavioral impairments, oxidative damage, histological alterations and apoptosis in focal cerebral ischemia-reperfusion model of rats.azadirachta indica linn. (meliaceae) has been used from ancient times as a remedy for various ailments. the present study was designed to investigate the antioxidant and anti-apoptotic properties of a. indica seed extract (ase) in transient middle cerebral artery occlusion (mcao) rat model. antioxidant potential of ase was determined in vitro. further, ase was evaluated against neurological deficits, histological alterations (ttc, cv and h&e) and oxidative damage (tbars, gsh and nitrite) in mcao ...201323187787
is a combine therapy of aqueous extract of azadirachta indica leaf (neem leaf) and chloroquine sulphate toxic to the histology of the rabbit cerebellum?herbal medication is commonly employed in treatment of diseases. aqueous extract of azadirachta indica leaf (a. indica) is commonly used in treatment of malaria by nigerians. most often, aqueous extract of a. indica leaf is taken in combination with chloroquine in order to cure malaria infection without knowledge of the side effect especially by the rural dwellers in nigeria.201123209976
potential use of neem leaf slurry as a sustainable dry season management strategy to control the malaria vector anopheles gambiae (diptera: culicidae) in west african villages.larval management of the malaria vector, anopheles gambiae giles s.s., has been successful in reducing disease transmission. however, pesticides are not affordable to farmers in remote villages in mali, and in other material resource poor countries. insect resistance to insecticides and nontarget toxicity pose additional problems. neem (azadirachta indica a. juss) is a tree with many beneficial, insect bioactive compounds, such as azadirachtin. we tested the hypothesis that neem leaf slurry is a ...201223270164
extracellular xylanase production by pleurotus species on lignocellulosic wastes under in vivo condition using novel pretreatment.the production of extracellular xylanase by three species of pleurotus species i.e. p. florida, p. flabellatus and p. sajor caju was studied under in vivo condition during their cultivation on pretreated lignocellulosic wastes. neem (azadirachta indica) oil and ashoka (saraca indica) leaves extract were used for pretreatment of paddy straw and wheat straw. between these two wastes, paddy straw pretreated with neem oil, supported better xylanase production than wheat straw. initially, xylanase pr ...201223273208
evaluating eco-friendly botanicals (natural plant extracts) as alternatives to synthetic fungicides.fungicides are widely used in conventional agriculture to control plant diseases. prolonged usage often poses health problems as modern society is becoming more health-conscious. penicillium digitatum, the cause of citrus green mould, is an important postharvest pathogen which causes serious losses annually. the disease is currently managed with synthetic fungicides. there is, however, a growing concern globally about the continuous use of synthetic chemicals on food crops because of their poten ...201223311787
neem leaf glycoprotein activates cd8(+) t cells to promote therapeutic anti-tumor immunity inhibiting the growth of mouse sarcoma.in spite of sufficient data on neem leaf glycoprotein (nlgp) as a prophylactic vaccine, little knowledge currently exists to support the use of nlgp as a therapeutic vaccine. treatment of mice bearing established sarcomas with nlgp (25 µg/mice/week subcutaneously for 4 weeks) resulted in tumor regression or dormancy (tumor free/regressor, 13/24 (nlgp), 4/24 (pbs)). evaluation of cd8(+) t cell status in blood, spleen, tdln, vdln and tumor revealed increase in cellular number. elevated expression ...201323326300
eemian interglacial reconstructed from a greenland folded ice core.efforts to extract a greenland ice core with a complete record of the eemian interglacial (130,000 to 115,000 years ago) have until now been unsuccessful. the response of the greenland ice sheet to the warmer-than-present climate of the eemian has thus remained unclear. here we present the new north greenland eemian ice drilling ('neem') ice core and show only a modest ice-sheet response to the strong warming in the early eemian. we reconstructed the eemian record from folded ice using globally ...201323344358
toxicological evaluation of neem (azadirachta indica) oil: acute and subacute toxicity.neem (azadirachta indica), popularly known as traditional medicine is a native plant in india. neem oil is a vegetable oil derived from seeds or fruits of the neem tree through pressing or solvent extraction, and largely used in popular medicine to have antifungal, antibacterial, antimalarial, antiparasitic, anti-inflammatory, as well as immunemodulatory properties in different animal species. in the present study, acute and 28-day subacute toxicity tests were carried out. in the acute toxicity ...201323353547
development of novel biodegradable au nanocomposite hydrogels based on wheat: for inactivation of bacteria.the design and fabrication of novel biodegradable gold nanocomposites hydrogels were developed as antibacterial agent. biodegradable gold nanocomposite hydrogels were developed by using acrylamide (am) and wheat protein isolate (wpi). the gold nanoparticles were prepared as a gold colloid by reducing haucl(4)·xh(2)o with leaf extracts of azadirachta indica (neem leaf) that formed hydrogel network. the characterization of developed biodegradable hydrogels were studied using fourier transforms inf ...201323399276
the efficacy of neem extract on four microorganisms responsible for causing dental caries viz streptococcus mutans, streptococcus salivarius, streptococcus mitis and streptococcus sanguis: an in vitro study.history and objectives: from the ancient time, neem used to be the traditional medicine for many diseases and was mainly used for cleaning the oral cavity. the incidence of dental caries was less a few decades ago but now the incidence of caries is very aggressive. this might be due to change in dietary habits, life style and more tendency toward processed food. the objective of this study is to find out the truth that if the neem is really efficacious against caries-inducing microorganisms, mai ...201223404001
green synthesis of small silver nanoparticles using geraniol and its cytotoxicity against fibrosarcoma-wehi 164.many reports have been published about the biogenesis of silver nanoparticles using several plant extracts such as pelargonium graveolens (p.graveolens- geranium) and azadirachta indica (neem) but the capacity of their natural reducing constituents to form silver nanoparticles has not yet been studied. in this research the synthesis of silver nanoparticles using geraniol has been investigated. we successfully synthesized uniformly dispersed silver nanoparticles with a uniform size and shape in t ...200923407598
the use of an extract of hypericum perforatum and azadirachta indica in advanced diabetic foot: an unexpected outcome.this is the first case reporting the results of using an extract of hypericum flowers (hypericum perforatum) and neem oil (azadirachta indica) in foot wounds with exposed bone in a patient with bilateral advanced diabetic ulcers. the effective use of this cheap treatment in patients with diabetic lesions on the feet, if confirmed in a wide controlled study, might allow the caregivers to take care of patients at home.201323413284
insecticidal and biting deterrent activity of rose-scented geranium (pelargonium spp.) essential oils and individual compounds against stephanitis pyrioides and aedes aegypti.rose-scented geranium, pelargonium spp., essential oils from the cultivars 'bourbon', 'china', 'egypt', 'rober's lemon rose' and 'frensham' were analyzed by gc-fid and gc-ms. a total of 136 compounds were identified from five essential oils, constituting 85.5-99.7% of the oils. essential oils and pure compounds were evaluated for their insecticidal activity against stephanitis pyrioides and larvicidal and biting deterrent activity against aedes aegypti.201323423995
ethnobotanical survey of malaria prophylactic remedies in odisha, india.to investigate the plants traditionally used for prevention of malaria in cuttack, gajapati and koraput districts of odisha state, eastern india.201323434608
a 90-day subchronic toxicity study of neem oil, a azadirachta indica oil, in mice.to determine the no-observed-adverse-effect level (noael) of exposure and target organs of neem oil for establishing safety criteria for human exposure, the subchronic toxicity study with neem oil in mice was evaluated. the mice (10 per sex for each dose) was orally administered with neem oil with the doses of 0 (to serve as a control), 177, 533 and 1600 mg/kg/day for 90 days. after the treatment period, observation of reversibility or persistence of any toxic effects, mice were continuously fed ...201323444337
molecular basis of 'hypoxic' breast cancer cell radio-sensitization: phytochemicals converge on radiation induced rel signaling.heterogeneously distributed hypoxic areas are a characteristic property of locally advanced breast cancers (bca) and generally associated with therapeutic resistance, metastases, and poor patient survival. about 50% of locally advanced bca, where radiotherapy is less effective are suggested to be due to hypoxic regions. in this study, we investigated the potential of bioactive phytochemicals in radio-sensitizing hypoxic bca cells.201323452621
removal of cadmium(ii) from wastewater using activated carbon prepared from agro industrial by-products.removal of cadmium from wastewater using activated carbons prepared from cocos nucifera (coconut) and azadirachta indica (neem) oilcakes-an agricultural solid by-product was investigated. batch experiments were performed to evaluate the effect of ph, agitation time, initial metal ion concentration and adsorbent dose on the cadmium sorption in coconut oil cake activated carbon (cocac) and neem oil cake activated carbon (nocac). the experiments demonstrated that the adsorption process corresponds ...201123505814
extract of azadirachta indica (neem) leaf induces apoptosis in 4t1 breast cancer balb/c mice.azadirachta indica (neem) has been used traditionally for many centuries. some impressive therapeutic qualities have been discovered. however, the therapeutic effect of neem leaf extract in 4t1 breast cancer has not been documented. the purpose of the present study is to investigate the therapeutic effect of ethanolic neem leaf extract in an in vivo 4t1 breast cancer model in mice.201123507990
sequence determination of a novel tripeptide isolated from the young leaves of azadirachta indica a. juss.the neem tree has long been recognized for its unique properties, both against insects and in improving human health. every part of the tree has been used as a traditional medicine for household remedy against various human ailments, from antiquity. although the occurrence of various phytochemicals in neem has been studied, we have identified the presence of a novel tripeptide in the young leaves of neem using a simple and inexpensive paper chromatographic method, detected by cu(ii)-ninhydrin re ...201323509470
genotoxicity assessment of nim-76 and its formulation (pessary) in an in vitro ames salmonella/microsome assay and in vivo mouse bone marrow micronucleus test.the possible genotoxic potential of nim-76, a volatile fraction obtained from neem oil, having promising contraceptive activity, as well as its formulation product, called pessary (7.5% nim-76 in polyethylene glycol), were evaluated in the ames assay and mouse bone marrow micronucleus (mn) assay. genotoxicity of nim-76 (0.1-1000 µg/plate) and pessary (0.1-10,000 µg/plate) were studied using the liquid preincubation protocol of the ames assay both in the presence and absence of s9. likewise, the ...201323527474
activated charcoal-mediated rna extraction method for azadirachta indica and plants highly rich in polyphenolics, polysaccharides and other complex secondary compounds.high quality rna is a primary requisite for numerous molecular biological applications but is difficult to isolate from several plants rich in polysaccharides, polyphenolics and other secondary metabolites. these compounds either bind with nucleic acids or often co-precipitate at the final step and many times cannot be removed by conventional methods and kits. addition of vinyl-pyrollidone polymers in extraction buffer efficiently removes polyphenolics to some extent, but, it failed in case of a ...201323537338
urushiol-induced contact dermatitis caused during shodhana (purificatory measures) of bhallataka (semecarpus anacardium linn.) fruit.bhallataka (semecarpus anacardium linn.; ancardiaceae) is mentioned under upavisha group in ayurvedic classics and it is described as a poisonous medicinal plant in drugs and cosmetics act (india), 1940. fruit of bhallataka is used either as a single drug or as an ingredient in many compound formulations of indian systems of medicine to cure many diseases. tarry oil present in the pericarp of the fruit causes blisters on contact. the major constituent of the tarry oil is anacardic acid and bhila ...201223559802
acaricidal activity of cymbopogon citratus and azadirachta indica against house dust mites.to examine the acaricidal effects of the essential oil of cymbopogon citratus leaf extract (lemongrass) and ethanolic azadirachta indica leaf extract (neem) against house dust mites dermatophagoides farinae (d. farinae) and dermatophagoides pteronyssinus (d. pteronyssinus).201123569794
toxicity profile of ethanolic extract of azadirachta indica stem bark in male wistar rats.to investigate the toxic implications of ethanolic stem bark extract of azadirachta indica (a. indica) at 50, 100, 200 and 300 mg/kg body weight in wistar rats.201223569852
biological activities and medicinal properties of gokhru (pedalium murex l.).bada gokhru (pedalium murex l.) is perhaps the most useful traditional medicinal plant in india. each part of the neem tree has some medicinal property and is thus commercially exploitable. during the last five decades, apart from the chemistry of the pedalium murex compounds, considerable progress has been achieved regarding the biological activity and medicinal applications of this plant. it is now considered as a valuable source of unique natural products for development of medicines against ...201223569975
the acaricidal efficacy of aqueous neem extract and ivermectin against sarcoptes scabiei var. cuniculi in experimentally infested rabbits.sarcoptes scabiei var. cuniculi is one of the most important veterinary ectoparasites in rabbits and results in considerable loss of weight, productivity, and wool quality. the acaricidal activity of aqueous leaf extract of neem (can) and ivermectin (ivr) were evaluated in vitro and in vivo against s. scabiei var. cuniculi. rabbits were classified into four groups (ten rabbits each). the first group (group 1) was designated as the negative control group. each rabbit of the other groups was exper ...201323572045
reclamation of tannery polluted soil through phytoremediation.the huge volume of sludge emanating from the tannery effluent treatment plants poses a serious environmental problem. phytoremediation is an emerging technology in which the plants are employed to reclamate the contaminated soil strewn with heavy metals (metalloids) and toxic compounds. this work focuses the impact of application of tannery sludge on biochemical properties of 6 months old tree saplings of azadirachta indica a. juss. (neem), melia azedarach linn. (wild neem) and leucaena leucocep ...200923572927
a comprehensive study on characterization of elite neem chemotypes through mycofloral, tissue-cultural, ecomorphological and molecular analyses using azadirachtin-a as a biomarker.azadirachtin-a (aza-a), a tetranortriterpenoid, found in minuscule amounts in the neem seed-kernels, has proved to be a potent biopesticide. given the vast biodiversity of azadirachta indica (neem) in india, this study is an overview of four main aspects that corroborate with each other in identifying elite neem chemotypes based on their aza-a content. these biomarkers included mycofloral, tissue-cultural, ecomorphometrical and molecular analyses on accessions from five ecogeographically differe ...201123572995
extraction and parametrization of grain boundary networks in glacier ice, using a dedicated method of automatic image analysis.microstructure analysis of polar ice cores is vital to understand the processes controlling the flow of polar ice on the microscale. this paper presents an automatic image processing framework for extraction and parametrization of grain boundary networks from images of the neem deep ice core. as cross-section images are acquired using controlled surface sublimation, grain boundaries and air inclusions appear dark, whereas the inside of grains appears grey. the initial segmentation step of the so ...201323573838
a comparative study of economical separation and aggregation properties of biologically capped and thiol functionalized gold nanoparticles: selecting the eco-friendly trojan horses for biological applications.we are presenting facile bio-fabrication of extremely stable gold nanoparticles (gnps) using medicinal plant azadirachta indica (commonly called neem) and its comparison with most commonly used glutathione (gsh) protected gnps in terms of stability under physiological conditions, seperation using density gradient centrifugation and aggregation properties in the solution. there was dual peak at 536 and 662 nm indicating the presence of non-spherical gnps including triangles, rods and hexagons in ...201323603039
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