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a novel lysozyme from xanthomonas oryzae phage varphixo411 active against xanthomonas and stenotrophomonas.in this study, a bacteriophage of xanthomonas oryzae pv. oryzae designated as varphixo411 was isolated. random sequencing of its genome revealed that it is closely related to another x. oryzae phage, xp10. a cloned fragment carries the lysozyme gene, lys411. the deduced protein, lys411, shares 92% identity with xp10 lysozyme, which contains an extra 46 aa at the n-terminus. lys411 shows over 40% identities to several other phage lysozymes. the his-tagged protein, lys411h, expressed in escherichi ...200616908188
assessment of pathogen frequency and resistance patterns among pediatric patient isolates: report from the 2004 sentry antimicrobial surveillance program on 3 continents.selecting empiric or directed therapy for pathogens isolated from pediatric patients can be problematic. many antimicrobial agents are not indicated for use in pediatric patients, and regional variations of resistance mechanisms have been reported. the purpose of this study was to analyze antimicrobial resistance patterns and pathogen occurrence rates in pediatric-aged patient infections on 3 continents using data from the sentry antimicrobial surveillance program. a total of 3537 clinical isola ...200616938419
[stenotrophomonas maltophilia bacteremia after endoscopic retrograde cholangiopancreatography]. 200616956545
central venous catheter-related stenotrophomonas maltophilia bacteraemia and associated relapsing bacteraemia in haematology and oncology patients.this retrospective study investigated the clinical significance and impact of stenotrophomonas maltophilia bacteraemia in 49 haematology and oncology patients at a tertiary referral medical centre in taipei between july 1999 and december 2003. sixteen patients had 24 episodes of central venous catheter (cvc)-related bacteraemia, with the main clinical characteristics being a nosocomial bacteraemia (100%), preceding antibiotic therapy (94%), bacteraemia developed in a general ward (87%), immunosu ...200616961635
cytotoxic activity of clinical stenotrophomonas maltophilia.to determine the potential virulence factors produced by culture supernatants of clinical isolates of stenotrophomonas maltophilia.200616965377
stenotrophomonas maltophilia resistant to trimethoprim-sulfamethoxazole: an increasing problem.stenotrophomonas maltophilia (s. maltophilia) has recently emerged as an important nosocomial pathogen. treatment of invasive infections caused by this organism is difficult as the bacterium is frequently resistant to a wide range of commonly used antimicrobials. trimethoprim-sulfamethoxazole (tmp - sxt) is recommended as the agent of choice for the treatment of s. maltophilia infections. however, the development of resistance to this antibiotic represents a real challenge to laboratorians and c ...200616978420
stenotrophomonas maltophilia and antibiotic use in german intensive care units: data from project sari (surveillance of antimicrobial use and antimicrobial resistance in german intensive care units).risk factors for the selection of stenotrophomonas maltophilia were analysed by correlating antimicrobial use and structure parameters (e.g. hospital type) with the incidence density of s. maltophilia and the percentage of s. maltophilia isolated from 39 intensive care units (icus). sari (surveillance of antimicrobial use and antimicrobial resistance in german intensive care units) is a prospective unit- and laboratory-based surveillance system that collects data on the 13 most important organis ...200616979794
infected vertebroplasty due to uncommon bacteria solved surgically: a rare and threatening life complication of a common procedure: report of a case and a review of the literature.case report.200616985448
streptococcal pd peritonitis--a 10-year review of one centre's experience.detailed descriptive studies focusing on streptococcal peritonitis in patients on peritoneal dialysis are lacking. most of the current information is available through isolated case reports.200617005523
frequency and predictors of colonization of the respiratory tract by vim-2-producing pseudomonas aeruginosa in patients of a newly established intensive care unit.the aim of this study was to examine the frequency and predictors of colonization of the respiratory tract by metallo-beta-lactamase (mbl)-producing gram-negative bacteria in patients admitted to a newly established intensive care unit (icu) of a tertiary care hospital. specimens of tracheobronchial aspirates for microbiological studies were obtained every day for the first 3 days of the icu stay and subsequently every third day for the rest of the icu stay. pcr analysis and nucleotide sequencin ...200617005794
ciprofloxacin-resistant gram-negative bacilli in the fecal microflora of children.the extent to which antibiotic-resistant bacteria are excreted by humans who have not been exposed to antibiotics is not known. children, who rarely receive fluoroquinolones, provide opportunities to assess the frequency of fecal excretion by fluoroquinolone-naïve hosts of fluoroquinolone-resistant gram-negative bacilli. fresh nondiarrheal stools from children were processed by screening them on agar containing ciprofloxacin to recover ciprofloxacin-resistant gram-negative bacilli. resistant iso ...200617005812
multiple-drug-resistant bacteria in patients with severe acute exacerbation of chronic obstructive pulmonary disease: prevalence, risk factors, and outcome.to determine prevalence, risk factors, and effect on outcome of multiple-drug-resistant (mdr) bacteria in patients with severe acute exacerbation of chronic obstructive pulmonary disease.200617012911
blooms of single bacterial species in a coastal lagoon of the southwestern atlantic ocean.we investigated seasonal differences in community structure and activity (leucine incorporation) of the planktonic bacterial assemblage in the freshwater and brackish-water zones of a shallow coastal lagoon of the southwestern atlantic ocean. alphaproteobacteria formed the dominant microbial group in both zones throughout the sampling period. after an intrusion of marine water, members of the sar11 lineage became abundant in the brackish-water zone. these bacteria were apparently distributed ove ...200617021206
is the prevalence of stenotrophomonas maltophilia isolation and nosocomial infection increasing in intensive care units?the study presented here was conducted over a period of 4 years (2001-2004) to investigate changes in the number of s. maltophilia isolates detected per 1,000 patient days and to look at the incidence density of nosocomial infections caused by s. maltophilia. the analysis was based on data provided by 34 german intensive care units participating continuously in the national project "surveillance of antimicrobial use and resistance in icus"; 31 of these icus reported nosocomial infections to the ...200617021867
intensive care unit-acquired stenotrophomonas maltophilia: incidence, risk factors, and outcome.the aim of this study was to determine incidence, risk factors, and impact on outcome of intensive care unit (icu)-acquired stenotrophomonas maltophilia.200617026755
nald encodes a second repressor of the mexab-oprm multidrug efflux operon of pseudomonas aeruginosa.the pseudomonas aeruginosa nald gene encodes a tetr family repressor with homology to the smet and ttgr repressors of the smedef and ttgabc multidrug efflux systems of stenotrophomonas maltophilia and pseudomonas putida, respectively. a sequence upstream of mexab-oprm and overlapping a second promoter for this efflux system was very similar to the smet and ttgr operator sequences, and nald binding to this region was, in fact, demonstrated. moreover, increased expression from this promoter was se ...200617028276
a ctx-m extended-spectrum beta-lactamase in pseudomonas aeruginosa and stenotrophomonas maltophilia. 200617030926
[identification of a strain nj2 hydroxylating imidacloprid and the transformed product].a strain named nj2 was isolated from the soils in nanjing area, and its resting cells transformed imi into a polar product. the strain was identified as stenotrophomonas maltophilia based on biomerieux vitek system and the analysis of 16s rdna sequence. mass spectrometry of the product extracted with an organic solvent and re-crystallization gave m/z value of 272 for the molecular ion while 256 for substrate imi. it demonstrated that the transformed product was the hydroxylated derivate of imi o ...200617037054
[isolation, identification and phylogenetic analysis of a pathogenic bacterium in channel catfish].a pathogenic bacterium (ccf00024) was isolated from the kidney and liver of the diseased channel catfish with acute epidemic disease. artificial infection proved that the bacterium was the pathogen of the disease. its morphological, physiological, biochemical characteristics and 16s rdna sequence analysis were studied. the isolated strain is an aerobic, non-fermentative bacterium. the bacteria are gram negative, rods, with polar multi-flagella; oxidase-negative, methyl-red-negative, lysine decar ...200617037072
nitrogen-fixing chemo-organotrophic bacteria isolated from cyanobacteria-deprived lichens and their ability to solubilize phosphate and to release amino acids and phytohormones.cyanobacteria-deprived lichens of the species canoparmelia caroliniana, canoparmelia crozalsiana, canoparmelia texana, parmotrema sancti-angeli and parmotrema tinctorum were screened for the presence of chemo-organotrophic nitrogen-fixing bacteria.200617040231
isolation of bacteria able to grow on both polyethylene glycol (peg) and polypropylene glycol (ppg) and their peg/ppg dehydrogenases.two bacterial consortia growing on a random copolymer of ethylene glycol and propylene glycol units were obtained by enrichment cultures from various microbial samples. six major strains included in both consortia were purified and identified as sphingomonads, pseudomonas sp. and stenotrophomonas maltophilia. three of them (sphingobium sp. strain ek-1, sphingopyxis macrogoltabida strain ey-1, and pseudomonas sp. strain pe-2) utilized both peg and polypropylene glycol (ppg) as a sole carbon sourc ...200717043822
preparation of 3-ketovalidoxylamine a c-n lyase substrate: n-p-nitrophenyl-3-ketovalidamine by stenotrophomonas maltrophilia cctcc m 204024.3-ketovalidoxylamine a c-n lyase is one of three key enzymes in the production of valienamine, which is a potent glucosidase inhibitor from validamycin a. n-p-nitrophenyl-3-ketovalidamine, used as the substrate of 3-ketovalidoxylamine a c-n lyase, was prepared from n-p-nitrophenylvalidamine with free cells of stenotrophomonas maltrophilia cctcc m 204024. the yield and selectivity of n-p-nitrophenyl-3-ketovalidamine from cells were improved by treatment with 10 mm ethylenediaminetetraacetic acid. ...200717058074
the immunomodulatory effect of antibiotics on the secretion of tumour necrosis factor alpha by peripheral blood mononuclear cells in response to stenotrophomonas maltophilia stimulation.some antibiotics have been shown to modify the host immune response. infection with stenotrophomonas maltophilia, is often difficult to treat due to multiresistance to antibiotics. the authors examined the effect of four commonly used antimicrobial agents (ciprofloxacin, ceftazidime, cotrimoxazole and piperacillin-tazobactam) on tumour necrosis factor alpha (tnf alpha) production by human peripheral blood mononuclear cells (pbmc) stimulated with heat-killed s maltophilia. cotrimoxazole was the o ...200617087095
aph(3')-iic, an aminoglycoside resistance determinant from stenotrophomonas maltophilia.we report the characterization of an intrinsic, chromosomally carried aph(3')-iic gene from stenotrophomonas maltophilia clinical isolate k279a, encoding an aminoglycoside phosphotransferase enzyme that significantly increases mics of kanamycin, neomycin, butirosin, and paromomycin when expressed in escherichia coli. disruption of aph(3')-iic in k279a results in decreased mics of these drugs.200717088477
bacterial diversity in aortic aneurysms determined by 16s ribosomal rna gene analysis.aortic aneurysms are common vascular conditions that cause considerable morbidity and mortality. understanding of the mechanisms involved in the pathogenesis of the condition remains limited. recently, infection has been suggested as possible contributor in the development of the disease. the aim of the present study was to examine aortic aneurysms for the presence of bacterial dna using polymerase chain reaction (pcr) targeting the 16s ribosomal rna (rrna) gene, followed by cloning and sequenci ...200617098542
cloning of mpd gene from a chlorpyrifos-degrading bacterium and use of this strain in bioremediation of contaminated soil.an effective chlorpyrifos-degrading bacterium (named strain yc-1) was isolated from the sludge of the wastewater treating system of an organophosphorus pesticides manufacturer. based on the results of phenotypic features, phylogenetic similarity of 16s rrna gene sequences and biolog test, strain yc-1 was identified as the genus stenotrophomonas. the isolate utilized chlorpyrifos as the sole source of carbon and phosphorus for its growth and hydrolyzed chlorpyrifos to 3,5,6-trichloro-2-pyridinol. ...200617107423
first reported infections caused by three newly described genera in the family xanthomonadaceae.members of the family of xanthomonadaceae are typically characterized as environmental organisms. with the exception of stenotrophomonas maltophilia, these organisms are infrequently implicated as human pathogens. we describe three cases of central venous catheter-associated bloodstream infections caused by dokdonella koreensis, aquimonas voraii, and a luteibacter sp., all newly named genera within the family xanthomonadaceae. the three patients all had histories of underlying hematological diso ...200717122001
characterization of small-colony-variant stenotrophomonas maltophilia isolated from the sputum specimens of five patients with cystic fibrosis.cystic fibrosis (cf) patients are predisposed to chronic respiratory infection by nonfermentative gram-negative bacilli, including stenotrophomonas maltophilia. s. maltophilia is highly resistant to most antibiotics, with the exception of sulfamethoxazole-trimethoprim (sxt). sxt-resistant s. maltophilia has been reported, but the mechanism of resistance is not well defined. repeated findings of suspected small-colony-variant (scv) s. maltophilia isolates from the sputa of five cf patients were c ...200717135443
multidrug-resistant organisms in cystic fibrosis: management and infection-control issues.chronic infection and inflammation are the hallmarks of cystic fibrosis lung disease. as cystic fibrosis patients are living longer owing to more intense treatment, multidrug-resistant organisms are being isolated increasingly from patients' respiratory tracts. while the adverse effects of pseudomonas aeruginosa and burkholderia cepacia complex are well described, less is known about the clinical significance of other emerging multidrug-resistant organisms, such as methicillin-resistant staphylo ...200617140357
bacteria associated with orchid roots and microbial production of auxin.associative bacteria of terrestrial (paphiopedilum appletonianum) and epiphytic (pholidota articulata) tropical orchids were investigated. microbial community of epiphytic plant differed from that of the terrestrial one. streptomyces, bacillus, pseudomonas, burkholderia, erwinia and nocardia strains populated paphiopedilum roots, whereas pseudomonas, flavobacterium, stenotrophomonas, pantoea, chryseobacterium, bacillus, agrobacterium, erwinia, burkholderia and paracoccus strains colonized pholid ...200717140781
meningitis caused by stenotrophomonas maltophilia: case report and review of the literature. 200217159399
capillary isoelectric focusing and fluorometric detection of proteins and microorganisms dynamically modified by poly(ethylene glycol) pyrenebutanoate.the nonionogenic pyrene-based tenside, poly(ethylene glycol) pyrenebutanoate, was prepared and applied in capillary isoelectric focusing with fluorometric detection. this dye was used here as a buffer additive in capillary isoelectric focusing for a dynamic modification of the sample of proteins and microorganisms. the values of the isoelectric points of the labeled bioanalytes were calculated with use of the fluorescent pi markers and were found comparable with pi of the native compounds. the m ...200617165837
serological cross-reactivity of environmental isolates of enterobacter, escherichia, stenotrophomonas, and aerococcus with shigella spp.-specific antisera.using protocols designed for the isolation of shigella from environmental freshwater samples from different regions of bangladesh, 11 bacterial strains giving rise to shigella-like colonies on selective agar plates and showing serological cross-reaction with shigella-specific antisera were isolated. phylogenetic analyses revealed that three of the isolates were most closely related to escherichia coli, four to enterobacter sp., two to stenotrophomonas, and two isolates belonged to the gram-posit ...200717171463
infectious keratitis caused by stenotrophomonas maltophilia and yeast simultaneously.to report a case of coinfection of the human cornea by stenotrophomonas maltophilia and yeast.200617172906
camphor-related self-inflicted keratoconjunctivitis complicating delusions of parasitosis.to report a case of camphor-related self-inflicted keratoconjunctivitis secondary to delusions of parasitosis.200617172913
influence of harvest bacterial contamination on autologous peripheral blood progenitor cells post-transplant.microbiological contamination of manipulated blood products, including hematopoietic progenitors obtained from peripheral blood, is an infrequent but persistent problem in transplant units. the relevance of such contamination in causing patient infection has been reported as insignificant, but the effect on the post-transplant course has not been well documented. we studied the incidence of bacterial contamination in autologous peripheral blood progenitor cell transplants in two of the bench pro ...200717173054
volatiles of bacterial antagonists inhibit mycelial growth of the plant pathogen rhizoctonia solani.bacterial antagonists are bacteria that negatively affect the growth of other organisms. many antagonists inhibit the growth of fungi by various mechanisms, e.g., secretion of lytic enzymes, siderophores and antibiotics. such inhibition of fungal growth may indirectly support plant growth. here, we demonstrate that small organic volatile compounds (vocs) emitted from bacterial antagonists negatively influence the mycelial growth of the soil-borne phytopathogenic fungus rhizoctonia solani kühn. s ...200717180381
the molecular epidemiology of stenotrophomonas maltophilia bacteraemia in a tertiary referral hospital in the united arab emirates 2000-2004.stenotrophomonas maltophilia is recognised as an important cause of nosocomial infection, especially in immunocompromised patients, resulting in significant morbidity and mortality. the treatment of s. maltophilia infection presents a therapeutic challenge. the precise modes of transmission of s. maltophilia in the hospital environment are not known and such knowledge is essential to target interventions to prevent spread. there are few published data on the patterns of nosocomial infection in t ...200617194302
stenotrophomonas maltophilia pneumonia in cancer patients without traditional risk factors for infection, 1997-2004.in order to elucidate the spectrum of stenotrophomonas maltophilia pneumonia in cancer patients without traditional risk factors, 44 cancer patients (cases) with s. maltophilia pneumonia in whom s. maltophilia pneumonia risk factors were not present were compared with two s. maltophilia pneumonia risk groups (controls) including 43 neutropenic non-intensive care unit (icu) and 21 non-neutropenic icu patients. the case and control patients had similar demographic and underlying clinical character ...200717200840
[short communication: prevalence of extended spectrum beta-lactamases in gram negative rods: data of 2001-2004 period].this study was undertaken to determine the prevalence of extended spectrum beta-lactamase (esbl) production by gram negative rods (gnrs) isolated from various clinical specimens (3.240 urine, 336 pus, 277 feces, 83 blood, 38 sputum) sent to our hospital laboratory, between 2001-2004. of isolated bacteria 71.4% were identified as escherichia coli, 11.1% were klebsiella spp, 4.2% were salmonella spp, 3.7% were pseudomonas spp, 3.5% were proteus spp, 2.9% were shigella spp, 2.3% were enterobacter s ...200617205693
in vitro activity of tigecycline against clinical isolates of acinetobacter baumannii and stenotrophomonas maltophilia. 200717213264
microbial communities in the larval midgut of laboratory and field populations of cotton bollworm (helicoverpa armigera).we compared the bacterial communities in the larval midgut of field and laboratory populations of a polyphagous pest, the cotton bollworm (helicoverpa armigera), using denaturing gradient gel electrophoresis (dgge) of amplified 16s rdna sequences and 16s library sequence analysis. dgge profiles and 16s rdna library sequence analysis indicated similar patterns of midgut microbial community structure and diversity: specific bacterial types existed in both populations, and a more diverse microbial ...200617215900
enhanced hydroxylation of imidacloprid by stenotrophomonas maltophilia upon addition of sucrose.sucrose's ability to promote the hydroxylation of imidacloprid (imi) by bacterium stenotrophomonas maltophilia strain cgmcc 1.1788 was examined. both growing culture and resting cells could transform imi into 5-hydroxy imi. adding 2% sucrose to the growing culture transformation broth and 5% sucrose to the resting cell transformation broth resulted in biotransformation yields, respectively, 2.5 and 9 times greater than without sucrose. in the growing culture transformation, sucrose increased bio ...200717216456
skin manifestation of stenotrophomonas maltophilia infection--a case report and review article.stenotrophomonas maltophilia is an aerobic gram-negative bacillus that is a frequent coloniser of fluids used in the hospital setting. it causes infection in immunosuppressed hosts, especially those who are neutropaenic, on chemotherapy and broad spectrum antibiotics. skin and soft tissue manifestations of stenotrophomonas maltophilia infection are becoming an increasingly recognised entity; the clinical spectrum ranges from mucocutaneous, skin to soft tissue infections.200617219003
immunostimulatory properties of the emerging pathogen stenotrophomonas maltophilia.stenotrophomonas maltophilia is a multiple-antibiotic-resistant opportunistic pathogen that is being isolated with increasing frequency from patients with health-care-associated infections and especially from patients with cystic fibrosis (cf). while clinicians feel compelled to treat infections involving this organism, its potential for virulence is not well established. we evaluated the immunostimulatory properties and overall virulence of clinical isolates of s. maltophilia using the well-cha ...200717220304
infectious complications of percutaneous central venous catheterization in pediatric patients: a spanish multicenter study.analysis of infectious complications and risk factors in percutaneous central venous catheters.200717235512
monitoring bacterial communities in raw milk and cheese by culture-dependent and -independent 16s rrna gene-based analyses.the diversity and dynamics of bacterial populations in saint-nectaire, a raw-milk, semihard cheese, were investigated using a dual culture-dependent and direct molecular approach combining single-strand conformation polymorphism (sscp) fingerprinting and sequencing of 16s rrna genes. the dominant clones, among 125 16s rrna genes isolated from milk, belonged to members of the firmicutes (58% of the total clones) affiliated mainly with the orders clostridiales and the lactobacillales, followed by ...200717259356
stability of a promiscuous plasmid in different hosts: no guarantee for a long-term relationship.broad-host-range (bhr) incp-1 plasmids have the ability to transfer between and replicate in nearly all species of the alpha-, beta- and gammaproteobacteria, but surprisingly few data are available on the stability of these plasmids in strains within their host range. moreover, even though molecular interactions between the bacterial host and its plasmid(s) exist, no systematic study to date has compared the stability of the same plasmid among different hosts. the goal of this study was to exami ...200717259616
munchausen syndrome by proxy and recurrent polymicrobial bacteremia. 200717259892
an acquired efflux system is responsible for copper resistance in xanthomonas strain ig-8 isolated from china.the genus xanthomonas contains plant pathogens exhibiting innate resistance to a range of antimicrobial agents. in other genera, multidrug resistance is mediated by a synergy between a low-permeability outer membrane and expression of a number of multidrug efflux systems. this report describes the isolation of a novel gene cluster xmersa from xanthomonas strain ig-8 that mediates copper chloride resistance. subsequent analysis of these genes showed that they were responsible for the high level o ...200717263848
identification of diazotrophs in the culturable bacterial community associated with roots of lasiurus sindicus, a perennial grass of thar desert, india.lasiurus sindicus is a highly nutritive, drought-tolerant, perennial grass that is endemic to the thar desert of rajasthan, india. analysis of 16s rrna coding genes of the bacterial isolates enriched in nitrogen-free semisolid medium, from the surface-sterilized roots of l. sindicus, showed predominance of gram-negative over gram-positive bacteria. according to comparative sequence analysis of 16s rdna sequence data, gram-positive bacteria with low gc content (staphylococcus warneri and bacillus ...200717264993
stenotrophomonas maltophilia pneumonia after bone marrow transplantation: case report with emphasis on the high-resolution ct findings.a 6-year-old female patient who underwent bone marrow transplantation because of fanconi anaemia presented with fever, dyspnoea and cough 17 days after the procedure. the physical examination revealed diffuse crackles. chest radiographs demonstrated diffuse alveolar opacities in both lungs. high-resolution ct showed a diffuse and bilateral lung lesion characterized by multifocal areas of air-space consolidation associated with ground-glass attenuation and small centrilobular nodules. the culture ...200717267464
stenotrophomonas maltophilia as a part of normal oral bacterial flora in captive snakes and its susceptibility to antibiotics.only little is known about normal oral bacterial flora in captive snakes containing stenotrophomonas maltophilia. this microbe has been reported as a causative agent of numerous infections in reptiles. therefore, the goal of the study was to detect its presence in the mouths of a significant number of healthy captive snakes and determining its susceptibility to antibiotics at 30 and 37 degrees c. the isolates were obtained in 1999-2005 from mouth swabs of 115 snakes of 12 genera and 22 species-m ...200717276020
enrichment and isolation of endosulfan degrading and detoxifying bacteria.in the present study, degradation of endosulfan by a mixed culture isolated from a pesticide-contaminated soil was studied in batch experiments. after two weeks of incubation, the mixed culture was able to degrade 73% and 81% of alpha and beta endosulfan respectively. endodiol was identified by gc/ms as degradation intermediate. the toxicity studies of endosulfan before and after degradation were carried out using micronucleus assay on human polymorphonuclear cells. the findings suggested that t ...200717289112
application of molecular methods for analysing the distribution and diversity of acetic acid bacteria in chilean vineyards.the presence of acetic acid bacteria populations on grape surfaces from several chilean valleys is reported. the bacteria were analysed at both the species and the strain level by molecular methods such as rflp-pcr 16s rrna gene, rflp-pcr its 16s-23s rrna gene regions and arbitrary primed (ap) pcr. our results show that there are limited numbers of species of acetic acid bacteria in the grapes and that there is a need for an enrichment medium before plating to recover the individual colonies. in ...200717289199
cystic fibrosis in adults.the aim of this cross-sectional retrospective study was to describe clinical and functional characteristics and the microbiological profile of an adult population with cystic fibrosis. the study was performed at the pulmonary diseases service of the hospital of the medical school of the state university of campinas. the charts of 54 adults (27 males, 27 females) with cystic fibrosis were reviewed. demographic, clinical, and microbiological data were collected. clinical and functional characteris ...200717294339
the impact of multidrug resistance on the outcomes of critically ill patients with gram-negative bacterial pneumonia.multidrug resistance (mdr) in gram-negative bacteria is on the rise, but its effect on patient outcomes is not well established. the outcomes of 129 adult surgical intensive care unit (sicu) patients treated for gram-negative pneumonia were evaluated in relation to demographics, bacterial etiology, and infections due to mdr bacteria (defined as resistant to all agents except for aminoglycosides and polymyxins). the mean (sd) age and acute physiology and chronic health evaluation (apache) ii scor ...200717300905
benzoic acid-degrading bacteria from the intestinal tract of macrotermes michaelseni sjöstedt.the intestinal tracts of termites host a wide variety of microbial symbionts, which have been implicated in degradative processes. in this study, a fungus-cultivating termite, macrotermes michaelseni was found to harbor 2.2 x 10(6) bacterial cells per ml of gut homogenates capable of degrading benzoic acid. two benzoic acid degrading bacteria were isolated from the highest dilution of gut homogenates in oxic media with benzoic acid as the sole carbon source. isolate cbc was related to stenotroph ...200717304624
isolation and identification of a novel valienamine-producing bacterium.to isolate, identify and assess valienamine production by a soil bacterial isolate from a wheat field in hangzhou, china.200717309634
emerging cystic fibrosis pathogens: incidence and antimicrobial resistance.we examined the frequency of isolation and the antimicrobial resistance of burkholderia cepacia complex, stenotrophomonas maltophilia and achromobacter xylosoxidans in cystic fibrosis patients from 2000 to 2004. strains susceptibility to tobramycin, piperacillin/tazobactam, imipenem, gentamicin, ciprofloxacin and ceftazidime was determined by disc diffusion assay. b. cepacia complex showed a very high resistance also to ciprofloxacin reaching 100% in 2004. s. maltophilia and a. xvylosoxidans sho ...200717319602
detection of dna from a range of bacterial species in the knee joints of dogs with inflammatory knee arthritis and associated degenerative anterior cruciate ligament rupture.mixtures of bacterial nucleic acids can often be detected in synovial joints affected with arthritis. we investigated the potential role of such mixtures of bacterial nucleic acids in the pathogenesis of arthritis in a naturally occurring canine model. dogs with a common inflammatory knee arthritis in which associated pathological degenerative anterior cruciate ligament (acl) rupture often develops were studied. synovial biopsies were obtained from 43 dogs with the naturally occurring acl ruptur ...200717320342
screening of the antibacterial effects of a variety of essential oils on microorganisms responsible for respiratory infections.the aim of this study was to examine the cytotoxicity and the antibacterial effects of a variety of essential oils on major respiratory tract pathogens. the cytotoxicity of 13 essential oils was evaluated on vero cells. the antibacterial activity was evaluated by the kirby bauer paper method, minimum inhibitory and bactericidal concentration against streptococcus pyogenes, agalactiae, pneumoniae and klebsiella pneumoniae, haemophilus influenzae, staphylococcus aureus and stenotrophomonas maltoph ...200717326042
increased incidence of class 1 integrons in trimethoprim/sulfamethoxazole-resistant clinical isolates of stenotrophomonas maltophilia. 200717329265
antimicrobial therapy for stenotrophomonas maltophilia infections.stenotrophomonas maltophilia has emerged as an important nosocomial pathogen capable of causing respiratory, bloodstream, and urinary infections. the treatment of nosocomial infections by s. maltophilia is difficult, as this pathogen shows high levels of intrinsic or acquired resistance to different antimicrobial agents, drastically reducing the antibiotic options available for treatment. intrinsic resistance may be due to reduced outer membrane permeability or to the multidrug efflux pumps. how ...200717334747
empirical antimicrobial treatment for chemotherapy-induced febrile neutropenia.febrile neutropenia in immunocompromised hosts is associated with a high mortality. empirical treatment in such cases is instituted to cover the common pathogens. generally, combination antibiotic treatment is used early in the febrile neutropenia phase. recent studies demonstrate that monotherapy with certain beta-lactam antibiotics can be equally effective. glycopeptide antibiotics are used in the absence of an adequate response to the initial antibiotics. empirical antifungal therapy may be g ...200717346939
comparison of etest, vitek and agar dilution for susceptibility testing of colistin.in total, 172 isolates of enterobacteriaceae, acinetobacter spp., pseudomonas aeruginosa and stenotrophomonas maltophilia were tested for susceptibility to colistin by agar dilution, etest and the vitek 2 system. isolates with a colistin mic < or =2 mg/l were considered to be susceptible. fifty-four (31%) gram-negative isolates were resistant to colistin. categorical agreement between agar dilution and etest was 87%, and between agar dilution and vitek 2 was 82%. based on the data obtained, the ...200717371537
pathogens in early-onset and late-onset intensive care unit-acquired pneumonia.to compare the type of pathogens isolated from patients with early-onset intensive care unit (icu)-acquired pneumonia with those isolated from patients with late-onset icu-acquired pneumonia and to study risk factors for the isolation of pathogens that are potentially resistant to multiple drugs.200717385143
physiological and phylogenetic characterization of a stable benzene-degrading, chlorate-reducing microbial community.a stable anoxic enrichment culture was obtained that degraded benzene with chlorate as an electron acceptor. the benzene degradation rate was 1.65 mm benzene per day, which is similar to reported aerobic benzene degradation rates but 20-1650 times higher than reported for anaerobic benzene degradation. denaturing gradient gel electrophoresis of part of the 16s rrna gene, cloning and sequencing showed that the culture had a stable composition after the seventh transfer. five bacterial clones were ...200717386037
[investigation of the structure of biofilms formed by sulfur cycle bacteria on metal matrices].the structure of biofilm formed by thiobacillus thioparus, stenotrophomonas maltophilia on the metal matrix was investigated. use of electron microscope-microanalysis showed that these bacteria can form biofilms 34-94 microm thick on metal different in their structure and elemental sulfur composition. a biofilm formed by mixed culture of thiobacillus thioparus and stenotrophomonas maltophilia had a developed interior structure and biofilm porosity. the use of the confocal laser scanning microsco ...200617388123
antibiotic binding to dizinc beta-lactamase l1 from stenotrophomonas maltophilia: scc-dftb/charmm and dft studies.a dizinc beta-lactamase (l1 from stenotrophomonas maltophilia) complexed with an antibiotic compound (moxalactam) has been studied using a hybrid quantum mechanical/molecular mechanical (qm/mm) approach. the qm region is described by the self-consistent charge-density functional tight binding (scc-dftb) model while the mm by charmm. the michaelis complex, which is constructed from a recent x-ray structure of the l1 enzyme with the hydrolyzed moxalactam, is simulated by molecular dynamics. the si ...200717388313
presumed pseudobacteremia outbreak resulting from contamination of proportional disinfectant dispenser.reported here are the microbiological and epidemiological details of a presumed outbreak of aerobic gram-negative bacilli infections affecting 19 hematological patients, which was traced to contaminated disinfectant. over a 5-month period, the following organisms were isolated from the blood cultures of 19 neutropenic patients: pseudomonas fluorescens (n = 13), achromobacter xylosoxidans (n = 12), comamonas testosteroni (n = 2) or stenotrophomonas maltophilia (n = 1). the affected patients were ...200717393202
faropenem: review of a new oral penem.faropenem medoxomil is a new orally administered penem antibiotic. its chiral tetrahydrofuran substituent at position c2 is responsible for its improved chemical stability and reduced cns effects, compared with imipenem. faropenem demonstrates broad-spectrum in vitro antimicrobial activity against many gram-positive and -negative aerobes and anaerobes, and is resistant to hydrolysis by nearly all beta-lactamases, including extended-spectrum beta-lactamases and ampc beta-lactamases. however, faro ...200717402834
bacterial reduction of selenium in coal mine tailings pond sediment.sediment from a storage facility for coal tailings solids was assessed for its capacity to reduce selenium (se) by native bacterial community. one se(6+)-reducing bacterium enterobacter hormaechei (tar11) and four se(4+)-reducing bacteria, klebsiella pneumoniae (tar1), pseudomonas fluorescens (tar3), stenotrophomonas maltophilia (tar6), and enterobacter amnigenus (tar8) were isolated from the sediment. enterobacter hormaechei removed 96% of the added se(6+) (0.92 mg l(-1)) from the effluents whe ...200717412898
relationship between immunosuppression and intensive care unit-acquired multidrug-resistant bacteria: a case-control study.to determine the relationship between immunosuppression and intensive care unit (icu)-acquired multidrug-resistant (mdr) bacteria.200717414081
[study on the structure and function of a stable methane-oxidizing mixed microbial consortium].from an agricultural sample taken in chongqing, a stable methane-oxidizing mixed microbial consortium was established by enrichment culture with methane as a sole source of carbon and energy. the mixed consortium showed high capability of phenol degradation and 1,2-epoxypropane production from propene. more than 99% of phenol at an initial concentration of 600mg/l could be degraded by the mixed microbial consortium after 11 h of cultivation. the productivity of 1, 2-epoxypropane could be increas ...200717436634
characterization of a novel t4-type stenotrophomonas maltophilia virulent phage smp14.stenotrophomonas maltophilia (sm), with most of the isolates being resistant to multidrugs, is an opportunistic bacterium causing nosocomial infections. in this study, a novel virulent sm phage, smp14, was characterized. electron microscopy showed that smp14 resembled members of myoviridae and adsorbed to poles of the host cells during infection. it lysed 37 of 87 clinical sm isolates in spot test, displayed a latent period of ca. 20 min, and had a burst size of ca. 150. its genome (estimated to ...200717440710
potential of the adhesion of bacteria isolated from drinking water to materials.heterotrophic bacteria (11 genera, 14 species, 25 putative strains) were isolated from drinking water, identified either biochemically or by partial 16s rdna gene sequencing and their adherence characteristics were determined by two methods: i. thermodynamic prediction of adhesion potential by measuring hydrophobicity (contact angle measurements) and ii. by measuring adherence to eight different substrata (asi 304 and 316 stainless steel, copper, polyvinyl chloride, polypropylene, polyethylene, ...200717440920
the efficacy of nonsurgical treatment of infected pancreatic necrosis.we conducted this study to evaluate the efficacy of nonsurgical treatment for patients with infected pancreatic necrosis (ipn).200717446837
removal of cadmium ions during stationary growth phase by an extremely cadmium-resistant strain of stenotrophomonas sp.stenotrophomonas sp. cd02 was isolated from a site that previously had been contaminated with high concentrations of the heavy metals cadmium (3 mg kg(-1)) and chromium (115 mg kg(-1)). this strain was able to grow on complex (luria bertani) medium containing high concentrations of cadmium ion (up to 4 mm). additionally, it could remove up to 80% of the dissolved ions but only after reaching stationary growth phase. strain cd02 also tolerated high concentrations of other heavy metals such as chr ...200717447550
surface charge influences enterococcal prevalence in mixed-species biofilms.to study the influence of 15 microbial isolates on the prevalence of charge-heterogeneous and charge-homogeneous enterococcus faecalis strains, all isolated from biliary stents, in mixed-species biofilms.200717448160
isolation and characterization of diuron-degrading bacteria from lotic surface water.the bacterial community structure of a diuron-degrading enrichment culture from lotic surface water samples was analyzed and the diuron-degrading strains were selected using a series of techniques combining temporal temperature gradient gel electrophoresis (ttge) of 16 s rdna gene v1-v3 variable regions, isolation of strains on agar plates, colony hybridization methods, and biodegradation assays. the ttge fingerprints revealed that diuron had a strong impact on bacterial community structure and ...200717450392
stenotrophomonas maltophilia infection of the central venous catheter and lysis of an old thrombosis in a post-bone marrow transplantation child: implications in the fibrinolytic process.in this report, we describe an 11-y-old girl who developed jugular venous thrombosis after allogeneic bone marrow transplantation for lymphoma and then experienced dissolution of the thrombosis following catheter-related stenothrophomonas maltophilia bactearemia. the lysis of the old thrombosis around the central venous catheter suggested a local fibrinolytic activity of s. maltophilia. the global fibrinolytic capacity (gfc) was also tested in vitro by using s. maltophilia cultures obtained from ...200717454909
successfully treated nosocomial stenotrophomonas maltophilia bacteremia following desensitization to trimethoprim-sulfamethoxazole.stenotrophomonas maltophilia has emerged as an important cause of morbidity and mortality in hospitalized patients. because trimethoprim-sulfamethoxazole (tmp-smx) remains the most effective drug for the treatment of s. maltophilia infections, desensitization should be considered in patients with hypersensitivity to tmp-smx.200717458682
selection for biocontrol bacteria antagonistic toward rosellinia necatrix by enrichment of competitive avocado root tip colonizers.biological control of soil-borne pathogens is frequently based on the application of antagonistic microorganisms selected solely for their ability to produce in vitro antifungal factors. the aim of this work was to select bacteria that efficiently colonize the roots of avocado plants and display antagonism towards rosellinia necatrix, the causal agent of avocado white root rot. a high frequency of antagonistic strains (ten isolates, 24.4%) was obtained using a novel procedure based on the select ...200717467245
diffusible signal factor-dependent cell-cell signaling and virulence in the nosocomial pathogen stenotrophomonas maltophilia.the genome of stenotrophomonas maltophilia encodes a cell-cell signaling system that is highly related to the diffusible signal factor (dsf)-dependent system of the phytopathogen xanthomonas campestris. here we show that in s. maltophilia, dsf signaling controls factors contributing to the virulence and antibiotic resistance of this important nosocomial pathogen.200717468254
disseminated cutaneous fusarium moniliforme infections in a leukemic child.a 5-year-old boy had a 10-month remission of acute lymphocytic leukemia (all) after chemotherapy. re-induction chemotherapy was performed for relapse of all. thereafter, he suffered from an episode of neutropenic fever with pneumonia. one week following control of the condition with antibiotics, a 1 x 1-cm, red, painful nodule appeared on the left thigh, which was initially suspected to be pseudomonas infection. parenteral ceftazidime and amikacin were administered, but persistent high fever, mi ...200717472677
clinical problem-solving. the drenched doctor - a 55-year-old male physician was seen in august because of a 1-week history of fever and night sweats. 200717476014
conjugative transfer of preferential utilization of aromatic compounds from pseudomonas putida csv86.pseudomonas putida csv86 utilizes naphthalene (nap), salicylate (sal), benzyl alcohol (balc), and methylnaphthalene (mn) preferentially over glucose. methylnaphthalene is metabolized by ring-hydroxylation as well as side-chain hydroxylation pathway. although the degradation property was found to be stable, the frequency of obtaining nap(-)sal(-)mn(-)balc(-) phenotype increased to 11% in the presence of curing agents. this property was transferred by conjugation to stenotrophomonas maltophilia cs ...200817487554
risk factors for infections with multidrug-resistant stenotrophomonas maltophilia in patients with cancer.stenotrophomonas maltophilia is responsible for an increasing number of infections, especially in hospitalized patients. therapy options are limited and trimethoprim/sulfamethoxazole (tmp/smx) is often the main treatment option for this infection. in the current study, the risk factors were determined for the emergence of multidrug-resistant (mdr) s. maltophilia.200717487860
thirteen-lined ground squirrels (spermophilus tridecemlineatus) harbor multiantibiotic-resistant bacteria.whether wild-caught animals used for biomedical research carry antibiotic-resistant bacteria is not well studied. thirteen- lined ground squirrels (spermophilus tridecemlineatus) are small mammals used to study hibernation. these animals are captured from the wild or are born in laboratory animal facilities to wild-caught mothers. because microorganisms harbored by 13-lined ground squirrels may be pathogenic to their caretakers and other laboratory animals, learning more about antibiotic resista ...200717487947
comparative review of the carbapenems.the carbapenems are beta-lactam antimicrobial agents with an exceptionally broad spectrum of activity. older carbapenems, such as imipenem, were often susceptible to degradation by the enzyme dehydropeptidase-1 (dhp-1) located in renal tubules and required co-administration with a dhp-1 inhibitor such as cilastatin. later additions to the class such as meropenem, ertapenem and doripenem demonstrated increased stability to dhp-1 and are administered without a dhp-1 inhibitor. like all beta-lactam ...200717488146
[primary intracerebral hemorrhage due to probable cerebral amyloid angiopathy complicated by brain abscess: case report].cerebral amyloid angiopathy (caa), in which deposition of amyloid within the arterial media and adventitia is the remarkable feature, causes spontaneous lober cerebral hemorrhage in elderly person, and some reports show the quite high occurrence rate of this entity among intracerebral hemorrhage in patents above 70 years old. brain abscess resulting from intracerebral hemorrhage is rare. to our knowledge, no report of such hemorrhage which is caused by caa has been published so far. we report a ...200717491345
[mesenteric vein thrombosis and protein c and s deficiency in a patient with chronic hepatitis c on treatment with interferon and ribavirin].we describe the case of a 67 year old male with chronic hepatitis c on treatment with pegylated interferon and ribavirin who, after two and a half months of combined treatment, presented with a picture of acute mesenteric vein thrombosis that required urgent surgery. it re-occurred several days later and was his cause of death. in the thrombophilia study carried out immediately after surgery a decrease in protein c and s was considered as a cause of hypercoagulability. protein c and s deficiency ...200717493436
evaluation of the colorimetric vitek 2 card for identification of gram-negative nonfermentative rods: comparison to 16s rrna gene sequencing.ninety strains of a collection of well-identified clinical isolates of gram-negative nonfermentative rods collected over a period of 5 years were evaluated using the new colorimetric vitek 2 card. the vitek 2 colorimetric system identified 53 (59%) of the isolates to the species level and 9 (10%) to the genus level; 28 (31%) isolates were misidentified. an algorithm combining the colorimetric vitek 2 card and 16s rrna gene sequencing for adequate identification of gram-negative nonfermentative r ...200717507509
composting and vermicomposting experiences in the treatment and bioconversion of asphaltens from the prestige oil spill.this work illustrates the effectiveness of composting and vermicomposting in degrading fuel-in-water emulsions from oil spills (chapapote), and the isolation of potentially useful microorganisms for its biodegradation. firstly, an alternative to the biodegradation of asphaltens from the prestige oil spill (still present in some chapapote rafts in the cantabrian coast) by means of the application of composting techniques to a microbial partnership acclimated to fuel-oil is offered. our aim is tha ...200817512195
genotype and antibiotic susceptibility patterns of pseudomonas aeruginosa and stenotrophomonas maltophilia isolated from cystic fibrosis patients.the purpose of this study was to type the pseudomonas aeruginosa and stenotrophomonas maltophilia isolates recovered from cystic fibrosis (cf) patients by random amplified polymorphic dna (rapd)-pcr and to determine the antibiotic susceptibility of these strains. p. aeruginosa (n=49), and s. maltophilia (n=11) isolates which had been recovered from 16 and 8 patients, respectively, during a 1-year period were investigated. three primers were used for rapd-pcr typing. antibiotic susceptibility tes ...200717515637
bloodstream infection caused by stenotrophomonas maltophilia in children.stenotrophomonas maltophilia, a multidrug resistant gram-negative pathogen, has become a more frequent cause of bloodstream infections (bsi). little is known about development of s. maltophilia bacteremia in children. the objective of this study was to define risk factors and outcomes associated with s. maltophilia bsi in children.200717529868
characterization of symbiotic and endophytic bacteria isolated from root nodules of herbaceous legumes grown in qinghai-tibet plateau and in other zones of china.qinghai-tibet plateau is the highest place in the world and the environment in that plateau is hard for animals and plants, with low temperature, low concentration of oxygen and high solar radiation. in this study, 61 root nodule isolates from vicia, oxytropis, medicago, melilotus and onobrychis species grown in qinghai-tibet plateau and in loess plateau were comparatively characterized. based upon the results of numerical taxonomy, ardra, aflp, dna-dna hybridization and 16s rdna sequencing, the ...200717541555
global emergence of trimethoprim/sulfamethoxazole resistance in stenotrophomonas maltophilia mediated by acquisition of sul genes.trimethoprim/sulfamethoxazole (tmp/smx) resistance remains a serious threat in the treatment of stenotrophomonas maltophilia infections. we analyzed an international collection of 55 s. maltophilia tmp/smx-sensitive (s) (n=30) and -resistant (r) (n=25) strains for integrons; sul1, sul2 and dhfr genes; and insertion element common region (iscr) elements. sul1, as part of a class 1 integron, was detected in 17 of 25 tmp/smx-r. nine tmp/smx-r strains carried sul2; 7 were on large plasmids. five tmp ...200717553270
emerging and unusual gram-negative infections in cystic fibrosis.people with cystic fibrosis (cf) have chronic airway infection and frequent exposure to antibiotics, which often leads to the emergence of resistant organisms. in addition to the development of multiresistance in common cf pathogens such as pseudomonas aeruginosa, several newer, inherently resistant gram-negative species are becoming more common, including burkholderia cepacia complex, stenotrophomonas maltophilia, achromobacter (alcaligenes) xylosoxidans, certain ralstonia species, and those wi ...200717562501
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