Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| [experimental herpetic infection in rabbits combined with immunodepression (author's transl)]. | the immunologic response to herpesvirus infection have been studied in rabbits receiving azathioprine before, after or simultaneously with the infection. the part of humoral depression in the complications following human kidney graft and the meaning of the viral antibodies rises in these patients are discussed. | 1975 | 1217787 |
| [development of optimal conditions for isolation of rhinoviruses]. | when 366 dispensary patients with respiratory diseases were examined in moscow during the period december 1969--may 1971, 49 strains of rhinoviruses were isolated out of which 21 strains were identified as type 48. using eagle's medium with a single set of amino acids and vitamins for isolation rhinoviruses were isolated in 4.7% of cases, empdoying eagle's medium with a double set of amino acids and vitamins in 16% of cases. virus isolation experiments were carried out in hela-bristol cell cultu ... | 1975 | 1220246 |
| efficacy of lyophilized turkey herpesvirus vaccine against marek's disease in broilers. | field trials involving approximately 1,250,000 chickens vaccinated with cell-free turkey herpesvirus (hvt) vaccine and 635,000 chickens vaccinated with cell-associated hvt vaccine howed both vaccines equally effective in reducing condemnations (attributable to marek's disease) at the poultry processing plant. vaccinated chickens were progeny of hens vaccinated at 1 day of age with cell-associated hvt vaccine. the maternal antibody did not interfere with the ability of either the cell-free or cel ... | 1975 | 1228717 |
| failure to reproduce the cytopathic effect and chorioallantoic membrane reactions of the so-called smon herpesvirus. | 1975 | 1241397 | |
| an animal model for dna-rna virus interaction based upon virological and histological findings. | the presence of endogenous oncornavirus and herpesvirus in guinea pigs has been established. the oncornavirus apparently is present in all guinea pigs but is expressed only under certain conditions. expression of the latent herpesvirus is generally age and strain dependent as is the development of spontaneous guinea pig leukemia. following special laboratory manipulation, expression of both virus types was accomplished in vitro. studies of the role played by these two virus types in the developm ... | 1976 | 1253155 |
| anti-herpes activity of adenine arabinoside monophosphate. | adenine arabinoside monophosphate (ara-amp) demonstrated antiviral activity equivalent to adenine arabinoside (ara-a) against herpesvirus types 1 and 2 in tissue culture. against established herpes virus type 1 epithelial keratitis in rabbits, ara-amp 2 per cent was comparable in efficacy to ara-a 3 per cent, and ara-amp 20 per cent was superior to ara-a 3 per cent. these results are especially significant in that ara-a's high solubility will permit (1) more concentrated formulations to be prese ... | 1976 | 1254407 |
| letter: recognition of herpesvirus antigen in biopsied skin from a patient with encephalitis. | 1976 | 1256475 | |
| proteins of herpesvirus type 2: i. virion, nonvirion, and antigenic polypeptides in infected cells. | 1976 | 1258358 | |
| cranial polyneuritis and bell palsy. | in view of the specific nature of the clinical and neurologic findings in bell palsy and other acute benign cranial neuritides, the neural component of cutaneous herpes simplex, the predilection of the herpesvirus for sensory nerves, and intrinsic behavior and immunologic interreactions of the herpesvirus within ganglion cells, it is suggested that (1) the entity that has been termed "idiopathic facial paralysis" be recognized as an acute benign cranial polyneuritis; and (2) other acute benign c ... | 1976 | 1267717 |
| letter: herpesvirus and cancer of the uterine cervix. | 1976 | 1268558 | |
| frequency of antibody to a virus-induced tumor-associated antigen (ag-4) in japanese sera from patients with cervical cancer and controls. | microquantitative complement fixation was used to detect antibody to ag-4, a tumor-associated herpesvirus type 2-induced antigen, in sera from japanese patients with cervical neoplasia. the prevalence of antibody to ag-4 in cervical cancer patients is 47% as compared to 7% in control women. that ag-4 antibody is less prevalent in japanese than in american cancer patients reflects the frequency of genital herpesvirus type 2 infections in japan. these findings confirm the association of ag-4 with ... | 1976 | 1268845 |
| antibody-dependent cell-mediated cytotoxicity in cows: comparison of effector cell activity against heterologous erthrocyte and herpesvirus-infected bovine target cells. | bovine peripheral blood leukocytes (pbl) and cells collected from the bovine mammary gland were assayed for antibody-dependent cell-mediated cytotoxicity (adcc) against chicken erythrocyte (crbc) and bovine herpesvirus-infected bovine kidney cell targets. bovine antisera were used to sensitize target cells. both pbl and mammary leukocytes expressed adcc, with the latter cell population having greater activity against both target cells. only the crbc target cells were killed by nonadherent pbl an ... | 1976 | 1270149 |
| sequence determination and genetic content of an 8.9-kb restriction fragment in the short unique region and the internal inverted repeat of marek's disease virus type 1 dna. | the dna sequence (8.9 kb) covering about 70% of the short unique region (us) and part of the short inverted repeat of the marek's disease virus type 1 ga strain was determined. computer analysis of the sequence showed the presence of nine potential open reading frames (orfs), consisting of more than 300 nucleotides in the us region. of these orfs, four were found to be homologous to us10 (minor virion protein), us3 (protein kinase), us2, and us6 (gd) in the us region of alpha-herpesvirus herpes ... | 1992 | 1282282 |
| study of bovine herpesvirus type 1 strains with monoclonal antibodies. | fourteen strains of bovine herpesvirus type 1 (bhv-1, ibrv) representing all three groups of bhv-1 (bhv-1.1, bhv-1.2, bhv-1.3) were studied by elisa using 106 monoclonal antibodies (mabs) produced against bhv-1. on the basis of the elisa, the mabs could be divided into three groups. the first group (40 mabs, 38%) reacted with all strains, the second group (43 mabs, 41%) with the respiratory and genital strains (bhv-1.1 and bhv-1.2) while the third group (23 mabs, 22%) only with the respiratory s ... | 1992 | 1284321 |
| detection of herpesvirus dna in the large intestine of patients with ulcerative colitis and crohn's disease using the nested polymerase chain reaction. | the prevalence of herpesvirus dna was examined in inflammatory bowel disease tissue. dna was extracted from resection and biopsy specimens of the large intestine from patients with ulcerative colitis (n = 21), patients with crohn's disease (n = 29), and patients with noninflammatory bowel disease (controls) (n = 21). the nested polymerase chain reaction was used to detect viral dna using primer pairs specific for either cytomegalovirus (cmv), herpes simplex virus 1 (hsv1), human herpesvirus 6 (h ... | 1992 | 1287131 |
| isolation of chicken anaemia virus from broiler chickens. | chicken anaemia virus (cav) infection was demonstrated, by both serology and virus isolation, in 1- to 6-week-old broiler chickens originated from various parent flocks in hungary. total losses in the broiler flocks were estimated at 7 to 8% and about 25% of the chickens failed to reach target body mass by the 7th week of life. the clinical signs, postmortem lesions and histopathological changes of the affected chickens were similar to those of naturally occurring cav-induced infectious anaemia ... | 1992 | 1298167 |
| identification of a unique marek's disease virus gene which encodes a 38-kilodalton phosphoprotein and is expressed in both lytically infected cells and latently infected lymphoblastoid tumor cells. | the identification of unique marek's disease (md) virus (mdv) antigens expressed not only in lytically infected cells but also in latently infected md lymphoblastoid tumor cell lines is important in understanding the molecular mechanisms of latency and transformation by mdv, an oncogenic lymphotropic herpesvirus of chickens. through cdna and nucleotide sequence analysis, an open reading frame (designated the pp38 orf) which encodes a predicted polypeptide of 290 amino acids was identified in bam ... | 1992 | 1309266 |
| a chronic illness characterized by fatigue, neurologic and immunologic disorders, and active human herpesvirus type 6 infection. | to conduct neurologic, immunologic, and virologic studies in patients with a chronic debilitating illness of acute onset. | 1992 | 1309285 |
| serology versus clinical signs or symptoms and main laboratory findings in the diagnosis of exanthema subitum (roseola infantum). | twenty-five patients with clinical exanthema subitum (roseola infantum) were enrolled into a study, where acute-phase and convalescent sera were examined for antibodies to human herpesvirus 6 (hhv-6), several other viruses, and other microbes. in addition, an acute-phase fecal specimen was examined for viruses by electron microscopy. confirmative or suggestive serologic evidence for recent hhv-6 infection was obtained in 23 (92%) cases. conversion to hhv-6 was found in 19 (76%), a diagnostic (gr ... | 1992 | 1309393 |
| anti-idiotypic antibodies to bovine herpesvirus-1 inhibit virus infection in cell cultures. | a panel of murine monoclonal antibodies (mabs) to bovine herpesvirus-1 (bhv-1) was prepared. three of them were neutralizing mabs and reacted against 130/75/50 kda, 77 kda, or 97 kda glycoproteins (gp). a fourth non-neutralizing mab recognized the 97 kda gp. competition radioimmunoassay demonstrated that each of the four mabs reacted against a different virus epitope. anti-idiotypic antibodies (anti-id) to the four mabs were produced in rabbits and purified by sequential immunoaffinity chromatog ... | 1992 | 1309639 |
| latency and reactivation of a thymidine kinase-negative bovine herpesvirus 1 deletion mutant. | a bovine herpesvirus 1 (bhv 1) mutant variant with a deletion in the thymidine kinase (tk) gene was assessed for its ability to establish latency and be reactivated in cattle. after treatment with dexamethasone, reactivated tk- bhv 1 was isolated from one of four cattle that received virus by intravenous inoculation only, and from four of four cattle that received virus by intranasal, intravaginal, and intravenous inoculation. results prove that tk- bhv 1 will establish latency and can be reacti ... | 1992 | 1309641 |
| human herpesvirus-6: tumorigenicity and tumor infiltrating lymphocytes. | we previously reported that human herpes-virus-6 (hhv-6) genome (strain gs) and a cloned subfragment (pzvh14) transfected nih 3t3 cells, induced foci of transformation with a frequency significantly above the background level. the transformed cells produced tumors in nude mice and immunocompetent (swiss) mice. in the current study, nude mice tumors were passed into swiss mice and more aggressive tumors (g-2ts and 14-2ts derived from hhv-6 genome and pzvh14 dna, respectively) were produced. 14-2t ... | 1992 | 1309683 |
| nucleotide sequence analysis of a homologue of herpes simplex virus type 1 gene us9 found in the genome of simian herpes b virus. | the 10k gene of simian herpes b virus (shbv) has been located and the nucleotide sequence determined. its relationship to homologous genes in other herpes-viruses has been examined. the shbv 10k gene exhibits a closer relationship to its homologue in hsv-1 than to those in the other viruses studied. nucleotide sequence identity of 61% was found between the hsv-1 and shbv 10k genes, and 57% identity was found between the corresponding predicted protein sequences. a comparison of the amino acid se ... | 1992 | 1309859 |
| glycoprotein iv of bovine herpesvirus 1-expressing cell line complements and rescues a conditionally lethal viral mutant. | glycoprotein iv (giv) of bovine herpesvirus 1 (bhv-1), a homolog of herpes simplex virus glycoprotein d, represents a major component of the viral envelope and a dominant immunogen. to analyze the functional role of giv during bhv-1 replication, cell line buiv3-7, which constitutively expresses giv, was constructed and used for the isolation of giv- bhv-1 mutant 80-221, in which the giv gene was replaced by a lacz expression cassette. on complementing giv-expressing cells, the giv- bhv-1 replica ... | 1992 | 1309917 |
| characterization of the regulatory functions of the equine herpesvirus 1 immediate-early gene product. | use of the translation-inhibiting drug cycloheximide has indicated that the equine herpesvirus 1 (ehv-1) immediate-early (ie) gene, the sole ehv-1 ie gene, encodes a major viral regulatory protein since ie mrna translation is a prerequisite for all further viral gene expression (w.l. gray, r. p. baumann, a. t. robertson, g. b. caughman, d. j. o'callaghan, and j. staczek, virology 158:79-87, 1987). an ehv-1 ie gene expression vector (psvie) in combination with chimeric ehv-1 promoter-chlorampheni ... | 1992 | 1309921 |
| herpesvirus saimiri encodes homologues of g protein-coupled receptors and cyclins. | herpesvirus saimiri (hvs) is a t-lymphotropic gammaherpesvirus which establishes asymptomatic infections in its natural host the squirrel monkey (saimiri sciureus), but which causes fatal lymphoproliferative diseases in other new world primates. sequencing studies show hvs is closely related to the human b-lymphotropic gammaherpesvirus epstein-barr virus (ebv). however, despite the general colinearity between the genomes of hvs and ebv, hvs contains genes not found in ebv or in the genomes of an ... | 1992 | 1309943 |
| glycoprotein-specific immune response in canine herpesvirus infection. | sera from dogs which were infected with canine herpesvirus (chv) were analyzed for their serological reactivities against virus-specific glycoproteins (gps). by sequential immunoblot analysis using sera from experimentally infected dogs, it was found that the antibody response to gp 145/112 appeared first followed by responses to gp47 and gp80. in addition, all sera from naturally infected dogs which showed neutralizing activity to chv reacted with gp 145/112, whereas 77% and 70% reacted with gp ... | 1992 | 1309997 |
| metabolism of host and viral mrnas in frog virus 3-infected cells. | treatment of purified frog virus 3 (fv3) with nonionic detergent and high salt released an endoribonucleolytic activity and confirmed earlier findings of a virion-associated endonuclease. this observation, coupled with evidence implicating host and viral message destabilization in herpesvirus and poxvirus biogenesis, raised the question of what role, if any, mrna degradation plays in fv3 replication. to answer this question, northern analyses of mock- and virus-infected cells were performed usin ... | 1992 | 1310177 |
| retrovirus insertion into herpesvirus in vitro and in vivo. | retroviruses and herpesviruses are naturally occurring pathogens of humans and animals. coinfection of the same host with both these viruses is common. we report here that a retrovirus can integrate directly into a herpesvirus genome. specifically, we demonstrate insertion of a nonacute retrovirus, reticuloendotheliosis virus (rev), into a herpesvirus, marek disease virus (mdv). both viruses are capable of inducing t lymphomas in chickens and often coexist in the same animal. rev dna integration ... | 1992 | 1310544 |
| clinical implications of herpesvirus infections in patients with aids. introduction. | 1992 | 1310568 | |
| clinical implications of herpesvirus infections in patients with aids. proceedings of a symposium. april 6, 1991, new york, new york. | 1992 | 1310569 | |
| protection against marek's disease by a fowlpox virus recombinant expressing the glycoprotein b of marek's disease virus. | fowlpox virus (fpv) recombinants expressing the glycoprotein b and the phosphorylated protein (pp38) of the ga strain of marek's disease virus (mdv) were assayed for their ability to protect chickens against challenge with virulent mdv. the recombinant fpv expressing the glycoprotein b gene elicited neutralizing antibodies against mdv, significantly reduced the level of cell-associated viremia, and, similar to the conventional herpesvirus of turkeys, protected chickens against challenge with the ... | 1992 | 1310755 |
| identification and characterization of a human herpesvirus 6 gene segment that trans activates the human immunodeficiency virus type 1 promoter. | human herpesvirus 6 (hhv-6) is a lymphotropic herpesvirus, and in vitro, hhv-6 can productively infect many of the same cell types as can human immunodeficiency virus (hiv). coinfection by both viruses in vitro can lead to both activation of the hiv promoter and acceleration of cytopathic effects. we have previously demonstrated that a large, 22.25-kb cloned hhv-6 fragment, pzvb70, can trans activate hiv promoter expression in vitro. in this study, we show that the pzvb70 fragment can trans acti ... | 1992 | 1310766 |
| human cd8+ herpes simplex virus-specific cytotoxic t-lymphocyte clones recognize diverse virion protein antigens. | the role of the hla class i-restricted, cd8+, herpes simplex virus (hsv)-specific cytotoxic t lymphocytes (ctl) in the control of human hsv infections is controversial because previous reports suggest that a substantial portion of the antigen-specific lytic response is mediated by cd4+ cells. to address this question directly, we isolated hsv-specific cd8+ ctl clones from a patient with recurrent genital herpes. these ctl were cloned by coculturing responder peripheral blood mononuclear cells (p ... | 1992 | 1310769 |
| herpesvirus saimiri oncogene stp-c488 encodes a phosphoprotein. | the stp-c488 open reading frame of herpesvirus saimiri encodes an oncoprotein that has transforming and tumor-inducing activities independent of the rest of the herpesvirus genome. stp-c488 protein has an unusual, membrane-associated, fibrous structure and is located primarily in perinuclear compartments. we now report that stp-c488 is phosphorylated in vivo. the phosphorylated form, which accounted for about 15% of stp-c488 in transformed cells, migrated slightly more slowly through sodium dode ... | 1992 | 1310780 |
| viral small nuclear ribonucleoproteins bind a protein implicated in messenger rna destabilization. | herpesvirus saimiri (hvs) is one of several primate viruses that carry genes for small rnas. the five h. saimiri-encoded u rnas (hsurs) are the most abundant viral transcripts expressed in transformed marmoset t lymphocytes. they assemble with host proteins common to spliceosomal small nuclear ribonucleoproteins (snrnps). hsurs 1, 2, and 5 exhibit sequences at their 5' ends identical to the auuua motif, which targets a number of protooncogene, cytokine, and lymphokine mrnas for rapid degradation ... | 1992 | 1311093 |
| induction of host cell protein synthesis by human herpesvirus 6. | we observed an increase in host cell protein synthesis in human cord blood lymphocytes (cbl) infected with human herpesvirus 6 relative to uninfected cultures. the magnitude of this effect could not be explained by a smaller decrease in cell number in the infected cultures. the induction of host cell protein synthesis by hhv-6 does not appear to be mediated by a stable soluble factor present in the infected cell culture supernatant. when cbl were infected with virus that had been exposed to ultr ... | 1992 | 1311135 |
| contribution of single genes within the unique short region of aujeszky's disease virus (suid herpesvirus type 1) to virulence, pathogenesis and immunogenicity. | pigs (3 and 10 weeks old) were infected intranasally with aujeszky's disease virus (adv) mutants that functionally lacked one of the non-essential genes in the unique short region of the genome (except the gene encoding the 11k protein). virus excretion in oropharyngeal fluid and disease symptoms were monitored. some pigs were killed to study pathogenesis, whereas others were challenged with virulent adv 8 weeks after the primary infection. mutants lacking protein kinase, or glycoproteins gp63 o ... | 1992 | 1311354 |
| characterization of enveloped tegument structures (l particles) produced by alphaherpesviruses: integrity of the tegument does not depend on the presence of capsid or envelope. | recent studies have shown that infection with herpes simplex virus type 1 (hsv-1) strain 17 generates in addition to virions a novel type of non-infectious particle. these particles, termed l particles, lack capsids and viral dna, and consist predominantly of tegument and envelope proteins. we show that l particle production is not restricted to one strain of hsv-1, and that pseudorabies virus and equine herpesvirus type 1 also release particles which are similar in composition to and morphologi ... | 1992 | 1311356 |
| analysis of human herpesvirus 6 glycoproteins recognized by monoclonal antibody ohv1. | a virus-specific glycoprotein (gp) from human herpes-virus 6 (hhv-6) was studied using the anti-hhv-6 monoclonal antibody ohv1. immunoprecipitation with extracts from infected cells revealed that the antibody recognized four glycosylated proteins (gps) with mrs of 106k, 102k, 65k and 63k under reducing conditions. however, only two gps, of 106k (gp106) and 102k, were detected under non-reducing conditions. pulse-chase experiments revealed that gp65 and gp63 were cleavage products of gp106 and gp ... | 1992 | 1311366 |
| herpesvirus infection of the male urethra identified by cytology. | 1992 | 1311891 | |
| herpesvirus antibody levels in the etiologic diagnosis of the acute retinal necrosis syndrome. | quantitative antibody levels to three herpesviruses in acute and chronic sera from six patients with clinical signs of the acute retinal necrosis syndrome were consistent with a specific etiologic diagnosis only in the two cases associated with cutaneous herpes zoster. available data on acute and convalescent antibody titers to herpes group viruses from these six patients in addition to data from 27 acute retinal necrosis cases from the literature disclosed that only 13 of the 33 patients (39%) ... | 1992 | 1311902 |
| [adenovirus infections in an emergency department]. | we have reviewed 108 emergency ward reports of patients with adenoviral positive cultures in "doce de octubre" hospital in madrid. our aim was to know the most frequent symptoms of the infection so that identification and treatment could be managed more efficiently in our department. cellular cultures were negative for other viruses such as cmv, enterovirus and herpesvirus. no bacteria were found in the throat cultures. of the children who were evaluated, 78% were younger than 3 years old. the m ... | 1992 | 1311905 |
| [infection by human herpesvirus type 6: epidemiology, immunopathology and clinical implications]. | the human herpesvirus type 6 has been discovered recently and is the object of numerous investigations. even though, its morphology is very close to the cytomegalovirus, its epidemiologic, immunopathologic and clinic characteristics are similar to the epstein-barr virus. like the latter, hhv-6 persists latent in the host during all his live, frequently relapsed and is ubiquitous. exanthema subitum in children and mononucleosis-like syndrome in adults have been attributed to acute hhv-6 infection ... | 1992 | 1312248 |
| release of tumor necrosis factor-alpha from bovine alveolar macrophages stimulated with bovine respiratory viruses and bacterial endotoxins. | the release of tumor necrosis factor-alpha (tnf-alpha) from cultured bovine alveolar macrophages (bam) was evaluated following stimulation of bam with bovine herpesvirus-1 (bhv-1), parainfluenza-3 (pi-3) virus, bovine respiratory syncytial virus (brsv), escherichia coli 0111:b4 endotoxin, pasteurella haemolytica type 1 endotoxin, pasteurella multocida endotoxin, and virus/endotoxin combinations. a cytotoxic assay system using georgia bovine kidney cells as targets was used to measure tnf-alpha a ... | 1992 | 1312264 |
| activation of bovine immunodeficiency-like virus expression by bovine herpesvirus type 1. | bovine immunodeficiency-like virus (biv) is a recently identified lentivirus that infects cattle. the virus has structural and genetic similarities to human hiv. the present study demonstrates that biv can be activated by bovine herpesvirus type 1 (bhv-1), a pathogen frequently associated with cattle diseases. activation of biv expression can be detected as increased biv reverse transcriptase activity, increased in the number of syncytia induced by biv, and increased in the steady state level of ... | 1992 | 1312280 |
| characterization of dexamethasone-induced reactivation of latent bovine herpesvirus 1. | synchronous reactivation of bovine herpesvirus type 1 in all latently infected rabbits was achieved following a single intravenous dose of dexamethasone. reactivated latent virus was first present in ocular secretions between 48 and 72 h post-dexamethasone treatment (pt). cell-free infectious virus, viral-antigen-containing neurons, and pathologic changes were detectable in trigeminal ganglia (tg) by 48 h pt. a shift from the viral transcriptional pattern characteristic of the latent state (late ... | 1992 | 1312639 |
| epstein-barr virus infection and replication in a human epithelial cell system. | epstein-barr virus, a human herpesvirus with oncogenic potential, infects two target tissues in vivo: b lymphocytes, where the infection is largely non-productive, and stratified squamous epithelium in which virus replication occurs. the interaction with b cells, initiated through virus binding to the b-cell surface molecule cr2 (ref. 4), has been studied in vitro and the virus 'latent' genes associated with b-cell growth transformation defined. by comparison, viral infection of epithelium remai ... | 1992 | 1312681 |
| biology of b virus in macaque and human hosts: a review. | b virus is a zoonotic alpha-herpesvirus enzootic in asian monkeys of the genus macaca. at least 25 cases of human disease caused by b virus have occurred to date, leading to death in 16 instances. advances in the technology available for the diagnosis of b virus infection and in the agents for its treatment are improving the prognosis for cases in human beings. efforts are under way at several institutions in the united states to establish b virus-free colonies of rhesus macaques for use in biom ... | 1992 | 1313312 |
| an immunoperoxidase method applied to the diagnosis of equine herpesvirus abortion, using conventional and rapid microwave techniques. | an indirect immunoperoxidase (imp) technique was applied to cryostat and paraffin sections of liver from ten aborted equine foetuses. equid herpesvirus type 1 (ehv-1) had been isolated from seven of them and ehv-4 from one: the remaining two were virologically negative and were not used as controls. in the eight virus-infected cases the immunostaining revealed foci of cells exhibiting a distinct brown cytoplasmic and inclusion body pigmentation. no specific signal was present in the non-infected ... | 1992 | 1313357 |
| pathological findings in horses dying during an outbreak of the paralytic form of equid herpesvirus type 1 (ehv-1) infection. | in 1988 an outbreak of the paralytic form of equid herpesvirus type 1 (ehv-1) infection occurred on a stud farm and several animals died. this provided an opportunity to perform detailed pathological investigations to gain insights into the pathogenesis of this spontaneous disease. two paretic mares, three foals, an aborted foetus and its non-paretic dam were examined. the endotheliotropism of the virus was clearly demonstrated by the use of an indirect immunoperoxidase (ip) stain. at autopsy, e ... | 1992 | 1313358 |
| igg antibodies to human herpesvirus-6 (hhv-6) in italian people. | sera from a sample of healthy italian people were tested in an indirect immunofluorescence assay (ifa) for reactivity to a human herpesvirus-6 (hhv-6) strain called cv, isolated from a baby with exanthem subitum (portolani et al., 1990). seropositivity values of 83.78%, 92.68% and 63.64% were found in subjects aged 3 months-6 years, 6-18 years and over 18 years respectively. sera from cordal blood, sera from subjects with evidence of active infection by cytomegalovirus (cmv) or by epstein-barr v ... | 1992 | 1313531 |
| stable growth transformation of human t lymphocytes by herpesvirus saimiri. | herpesvirus saimiri induces t-cell lymphomas in various species of new world monkeys and in rabbits, and it is able to immortalize monkey t lymphocytes in vitro. sequences responsible for these effects have been localized to a region of the genome that varies significantly among the virus subgroups a, b, and c. we now report that infection of human blood lymphocytes and thymocytes with strains of subgroup c, in contrast to viruses of the other subgroups, yields continuously proliferating t-cell ... | 1992 | 1313581 |
| [the polymerase chain reaction (pcr) for the detection of dna of equine herpesviruses 1 and 4]. | formalin-fixed and paraplast-embedded tissue samples of 42 aborted equine fetuses were examined by polymerase chain reaction for the presence of equine herpesvirus dna. the used set of primers was located in the glycoprotein 13 open reading frame and allowed the amplification of both ehv 1 und ehv 4. by cleaving pattern analysis after hinf i digestion ehv 1 could be distinguished from ehv 4. in 9 of the cases investigated ehv 1-dna was detected. this finding is in absolute context with the resul ... | 1992 | 1313672 |
| establishment of a genomic bank of bovine herpesvirus 1 using a novel positive selection plasmid vector. | a small positive selection cloning vector, designated psiig1, suitable for the construction of genomic banks in e. coli is described and used for the establishment of a bank of the bovine herpesvirus 1 genome. hybrid transformants are directly selected on agar plates containing ampicillin. the vector is based on the replicon of r1 and has a lambda pr promotor inserted upstream of the replication control genes. the vector has an uncontrolled (runaway) replication and is lethal to the host cell un ... | 1992 | 1313826 |
| human herpesvirus-6. | 1992 | 1313880 | |
| stable rescue of a glycoprotein gii deletion mutant of pseudorabies virus by glycoprotein gi of bovine herpesvirus 1. | glycoproteins homologous to glycoprotein b (gb) of herpes simplex virus constitute the most highly conserved group of herpesvirus glycoproteins. this strong conservation of amino acid sequences might be indicative of a common functional role. indeed, gb homologs have been implicated in the processes of viral entry and virus-mediated cell-cell fusion. recently, we showed that pseudorabies virus (prv) lacking the essential gb-homologous glycoprotein gii could be propagated on a cell line expressin ... | 1992 | 1313900 |
| immediate-early rna 2.9 and early rna 2.6 of bovine herpesvirus 1 are 3' coterminal and encode a putative zinc finger transactivator protein. | bovine herpesvirus 1 (bhv-1) contains three major immediate-early (ie) genes involved in regulation of the productive cycle of replication. two spliced ie rnas, ier4.2 (4.2 kb) and ier2.9 (2.9 kb), are under the control of a single promoter; ier1.7 (1.7 kb) is transcribed from a different promoter in the opposite direction. examining the kinetics of transcription, we found that the ier4.2/2.9 promoter was turned off at the end of the ie period. an alternative promoter became active, directing sy ... | 1992 | 1313901 |
| monoclonal antibodies for the identification of herpesvirus simiae (b virus). | to differentiate between b virus and hsv isolates from monkeys and man monoclonal antibodies (mabs) were produced to herpesvirus simiae (b virus) and herpes simplex type 1 and 2 (hsv-1 and hsv-2). mabs were tested by indirect immunofluorescence (ifat) for reactivity against herpesviruses from asiatic monkeys (b virus), african monkeys (sa 8 virus), and man (hsv-1, hsv-2, varicella-zoster virus, cytomegalovirus, and epstein-barr virus). mabs could be divided into groups a-e displaying specific re ... | 1992 | 1314049 |
| pathogenesis of equine herpesvirus-1 in specific pathogen-free foals: primary and secondary infections and reactivation. | six specific pathogen-free foals shown to be free of equine herpesvirus-1 and 4 (ehv-1 and -4) and lacking in maternally-derived antibodies were used to investigate the pathogenesis of ehv-1 in horses. following primary intranasal inoculation with ehv-1 all foals showed signs of a mild, self-limiting upper respiratory tract infection. a leucopenia was observed, comprising both a lymphopenia and neutropenia. virus was isolated from nasal mucus and buffy coat cells over several days during the cli ... | 1992 | 1314051 |
| comparison of herpesviruses isolated from reindeer, goats, and cattle by restriction endonuclease analysis. | a genomic comparison of bovine herpesvirus 1 (bhv-1), caprine herpesvirus (chv-2) and reindeer herpesvirus (rhv), was performed using 5 restriction endonucleases. cross neutralization of these three herpesviruses showed that bhv-1 and chv-2 had a relatively low degree of cross reaction with heterologous viruses. rhv showed a higher degree of such cross reactivity. the restriction endonuclease analyses showed that the migration patterns of the dna segments were different for the three groups of h ... | 1992 | 1314052 |
| reinfection and reactivation of equine herpesvirus-1 in the mouse. | balb/c mice were inoculated with equine herpesvirus-1 (ehv-1) by the intranasal (i.n.) route. mice developed respiratory signs; virus replication occurred in the respiratory tract and viraemia was detected; some mice died. recovered mice were given a second inoculation with the same strain 5 months later. following the second infection no mice died, however, virus replication was again observed in the respiratory tract and viraemia was detected once more. administration of an antiviral agent dur ... | 1992 | 1314054 |
| human herpesvirus type 6: review. | human herpesvirus type 6 (hhv-6), a newly recognized human herpesvirus first described in 1986, is morphologically similar to other herpesviruses but is distinguishable from all of them by some unique in vitro biological effects, specific antigenic analysis, and patterns of endonuclease restriction digests of dna. in vitro hhv-6 exhibits tropism mainly for t lymphocytes, but it also infects other cells, including b lymphocytes, monocytes-macrophages, glial cells, and fibroblasts. because hhv-6 c ... | 1992 | 1314102 |
| vaccination of mares against equine herpesvirus-1. | 1992 | 1314446 | |
| relationship of bovine herpesvirus 1 immediate-early, early, and late gene expression to host cellular gene transcription. | bovine herpesvirus 1 (bhv-1) gene expression was examined by rna blot hybridization using clones representing immediate-early, early, and late genes. an immediate-early protein gene probe hybridized with two transcripts, 3.4 and 5.8 kb, expressed by infected cells in the presence of cycloheximide (ch). during infection of cells without metabolic inhibitors these transcripts were detected as early as 2 hr postinfection (p.i.) and accumulated to 8 hr p.i. the early gene probe, thymidine kinase, hy ... | 1992 | 1314450 |
| induction of a mucosal barrier to bovine herpesvirus 1 replication in cattle. | current vaccines for human and animal herpesviruses engender an immunity that may ameliorate disease but generally fails to prevent infection, latency, reactivation from latency, or spread through a population. by administering intranasally to cattle bovine herpesvirus type 1 virion envelope proteins combined with the potent mucosal immune system adjuvant, cholera toxin b subunit, we engendered a local antibody response that acted as a barrier to infection of mucosal epithelial cells and thereby ... | 1992 | 1314454 |
| analysis of nucleotide sequence of the rightmost 43 kbp of herpesvirus saimiri (hvs) l-dna: general conservation of genetic organization between hvs and epstein-barr virus. | we present an analysis of 43,658 bp of contiguous nucleotide sequence comprising the right terminal region (conventional orientation) of the unique protein-coding component (l-dna) of the herpesvirus saimiri (hvs) genome. within this region lie the genes encoding the 160-kda virion protein, which is homologous to the 140-kda membrane antigen of epstein-barr virus (ebv), thymidylate synthase (ts), and the immediate-early (ie) 52-kda protein which is homologous to the ebv bmlf1 product. the 160-kd ... | 1992 | 1314457 |
| epithelial intracytoplasmic herpes viral inclusions associated with an outbreak of duck virus enteritis. | several muscovy ducks from a free-roaming flock of 65 muscovy and mallard ducks died over a 3-week period. three muscovy ducks were necropsied. gross and microscopic changes were compatible with duck virus enteritis, and the virus was isolated. in addition to intranuclear viral inclusion bodies in several tissues, intracytoplasmic inclusion bodies were present in esophageal and cloacal epithelium. by electron microscopy, the membrane-bound intracytoplasmic inclusions were found to contain envelo ... | 1992 | 1314555 |
| in vitro suppression of bone marrow progenitor cell differentiation by human herpesvirus 6 infection. | suppression of marrow function may be one of the most serious effects of human herpesvirus 6 (hhv-6) infection in marrow transplant patients. in this study, normal bone marrow mononuclear cells were infected in vitro with hhv-6, and a methylcellulose-based colony formation assay was used to evaluate the impact of the infection on marrow cell differentiation and proliferation. results demonstrated that the outgrowth of colony-forming units of granulocyte and macrophage lineages (cfu-gm) was decre ... | 1992 | 1314872 |
| marek disease virus encodes a basic-leucine zipper gene resembling the fos/jun oncogenes that is highly expressed in lymphoblastoid tumors. | marek disease virus (mdv) is a herpesvirus of chickens that induces t lymphomas within 3 weeks of infection. the short latency and polyclonal nature of mdv-induced tumors have suggested that the virus may encode one or more direct-acting oncogenes. to date, however, no mdv-specific tumor antigens or candidate transforming genes have been demonstrated. in this paper, we report the identification of a mdv gene encoding a protein with homology to the leucine-zipper class of nuclear oncogenes. it al ... | 1992 | 1315048 |
| expression of porcine pseudorabies virus genes by a bovine herpesvirus-1 (infectious bovine rhinotracheitis virus) vector. | recombinant dna techniques were used to insert foreign genes into bovine herpesvirus-1 [infectious bovine rhinotracheitis virus (ibrv)] vectors which were attenuated by deletion and/or insertion mutations in the ibrv thymidine kinase (tk) gene. in one recombinant, the regulatory and coding sequences of the late pseudorabies virus (prv) glycoprotein giii gene, were inserted into the early ibrv tk gene. this recombinant efficiently expressed the prv giii gene indicating that immediate early ibrv p ... | 1992 | 1315133 |
| gordon memorial lecture. chicken neoplasia--a model for cancer research. | 1. the use of animal models has been immensely important for the advancement of our knowledge of the aetiology and pathogenesis of human diseases, including neoplasia. 2. viruses, as oncogenic agents, were first described in the early 1900s when cell-free filtrates were used experimentally to transmit leukemias and sarcomas in chickens. in more recent years, studies with avian leukosis/sarcoma viruses have led the field in attempts to establish the genetic and molecular basis of viral oncogenesi ... | 1992 | 1315185 |
| primary human herpesvirus 6 infection in young children. | human herpesvirus 6 (hhv-6) is a recently discovered virus that, on the basis of serologic evidence, appears to infect most children by the age of three years. however, the clinical manifestations of primary hhv-6 infection have not been well defined. | 1992 | 1315416 |
| human herpesvirus 6: the virus and the search for its role as a human pathogen. | 1992 | 1315478 | |
| herpesviruses--immune escape artists? | viral persistence depends on the successful avoidance of the host's immunologic surveillance system. this review, which focuses specifically on the herpesviruses, delineates several possible strategies for evading or delaying the immune response. one strategy common to all herpesviruses is the establishment of latency, a state in which the virus may be partially or even completely hidden from the immune system. other proposed mechanisms of immune evasion include interaction of the virus with com ... | 1992 | 1315587 |
| axonal and transsynaptic (transneuronal) spread of herpesvirus simiae (b virus) in experimentally infected mice. | in order to study the pathogenesis of b virus infection of the nervous system, newborn and young mice were inoculated by four different routes: 1. intramuscular (i.m.) in the forelimb; 2. i.m. in the hindlimb; 3. subcutaneous (s.c.) in the abdominal wall; 4. intraperitoneal (i.p.). spread of virus was followed by immunohistochemical demonstration of viral antigen in tissue sections of the peripheral and central nervous system. three distinct patterns emerged: 1. after i.m. limb inoculations, vir ... | 1992 | 1315601 |
| a herpesvirus regulatory protein appears to act post-transcriptionally by affecting mrna processing. | post-transcriptional control mechanisms play an important role in regulating gene expression for a number of viruses, especially in the regulation of late gene products. in this study we have investigated the mode of action of icp27, an immediate-early regulatory protein of herpes simplex virus 1 (hsv-1) required for late gene expression. transfection experiments have demonstrated that icp27 can activate or repress expression depending on the target gene. here, we show that the regulatory activi ... | 1992 | 1315705 |
| human herpesvirus-6 dna in cerebrospinal fluid of a child with exanthem subitum and meningoencephalitis. | the involvement of human herpesvirus-6 (hhv-6) and herpes simplex virus infections was evaluated virologically and serologically in a 13-month-old girl with meningoencephalitic illness occurring in the pre-eruptive stage of exanthem subitum. an isolation of hhv-6 from blood and seroconversion to the virus confirmed the primary infection with the virus. hhv-6 gene sequences were detected in cerebrospinal fluid of acute stage of the disease by polymerase chain reaction. there was no evidence of he ... | 1992 | 1315948 |
| nucleotide sequence of hsur 6 and hsur 7, two small rnas of herpesvirus saimiri. | 1992 | 1315960 | |
| primary human herpesvirus 6 infections in children: a prospective serologic study. | 1992 | 1316414 | |
| new member of the multigene family of complement control proteins in herpesvirus saimiri. | a number of glycoproteins are regulators of the complement cascade and prevent damage to cells by inappropriate activation of complement. in humans, all of them are encoded by a multigene family on chromosome i and share a characteristic structural feature, the short consensus repeats of about 61 amino acids with a constant framework of cysteine, proline, and tryptophan. we found the gene for glycoproteins of analogous structure in herpesvirus saimiri, a t-lymphotropic tumor virus of new world p ... | 1992 | 1316492 |
| geographic/ethnic differences in human herpesvirus-6 antibody patterns. | serum samples from healthy adults in four geographic/ethnic groups (ghanaian blacks, malaysian chinese, malaysian indians and united states caucasians) were tested under code for antibodies to human herpesvirus-6 (hhv-6). the prevalence and titer of hhv-6 antibody in the healthy ghanaians were significantly higher than in the malaysian chinese; united states caucasians and malaysian indians had intermediate prevalence and titer of antibodies. thus far, no specific differences in hhv-6-associated ... | 1992 | 1316534 |
| characterization of the glycoprotein d gene products of equine herpesvirus 1 using a prokaryotic cell expression vector. | the gene encoding equine herpesvirus 1 (equine abortion virus; ehv-1) glycoprotein d was engineered into the prokaryotic vector pex, and expressed as a beta-galactosidase fusion product, which was recognized by pooled equine sera and anti-ehv-1 rabbit sera. antibodies raised against the ehv-1 gd fusion product identified strong bands in infected cells at 66 and 68 k and at 138 k in purified virus, thus characterizing the several forms of this major envelope glycoprotein which is an important can ... | 1992 | 1316667 |
| open reading frames encoding a protein kinase, homolog of glycoprotein gx of pseudorabies virus, and a novel glycoprotein map within the unique short segment of equine herpesvirus type 1. | dna sequence analysis of the unique short (us) segment of the genome of equine herpesvirus type 1 kentucky a strain (ehv-1) by our laboratory and strains kentucky d and ab1 by other workers identifies a total of nine open reading frames (orf). in this report, we present the dna sequence of three of these newly identified orfs, designated eus 2, eus 3, and eus 4. the eus 2 orf is 1146 nucleotides (nt) in length and encodes a potential protein of 382 amino acids. cis-regulatory sequences upstream ... | 1992 | 1316673 |
| identification and characterization of an equine herpesvirus 1 late gene encoding a potential zinc finger. | in this report, we present the dna sequence and transcriptional characterization of a gene (ir5) that maps within each of the inverted repeat (ir) segments of the equine herpesvirus type 1 (ehv-1) genome. the ir5 open reading frame (orf) is located within both ir sequences (nucleotides 9932-10,642 of the ir). dna sequence analyses of the ir5 gene region revealed an orf of 236 amino acids (24,793 da) that showed significant homology to orf64 of varicella-zoster virus and orf3 of ehv-4 both of whi ... | 1992 | 1316680 |
| detection of infectious laryngotracheitis virus infected cells with cloned dna probes. | a genomic library of infectious laryngotracheitis virus (iltv) dna bamh1 fragments was prepared and two cloned fragments were evaluated for their potential as probes for the detection of iltv infected cells. the virus was purified by a modified sucrose density gradient procedure for the isolation of pure iltv dna. a genomic library was constructed using bamh1-digested iltv dna and pgem7 as a vector. a 1.1 kb cloned bamh1 fragment of iltv dna was tested in a slot or dot blot assay for the detecti ... | 1992 | 1316798 |
| simian varicella virus: characterization of virion and infected cell polypeptides and the antigenic cross-reactivity with varicella-zoster virus. | simian varicella virus (svv) causes a varicella-like disease in non-human primates. in this study, svv virions were purified from svv-infected bsc-1 cells by zonal and differential gradient centrifugation and the virion polypeptide composition was analysed by sds-page. svv virions had a buoyant density of 1.21 g/ml, identical to the value obtained for varicella-zoster virus (vzv) virions purified by the same method. electron microscopy of the concentrated svv virions revealed characteristic herp ... | 1992 | 1316940 |
| identification of the equine herpesvirus type 1 glycoprotein 17/18 as a homologue of herpes simplex virus glycoprotein d. | the dna sequence of the equine herpesvirus type 1 (ehv-1) gd gene homologue has been determined for the strain ab1 and compared with previously published sequences. a portion of the gene has been located to a region of the genome which also encodes homologues of the herpes simplex virus type 1 genes for ge and gi and is known to encode an epitope of the virion protein gp17/18. analysis of the ehv-1 strain kentucky a (kya) by dna hybridization showed the presence of a gd gene homologue and establ ... | 1992 | 1316942 |
| identification and characterization of glycoprotein gp1 of bovine herpesvirus type 4. | three major bovine herpesvirus type 4 (bhv-4) glycoproteins have been described previously. by using monoclonal antibodies produced against bhv-4 envelope proteins from which the three major antigens had been removed by immunoaffinity, a fourth glycoprotein was identified. this protein (gp1) has a high mr (greater than 300k), is detected about 8 h post-inoculation of infected cells and is strictly expressed as a gamma protein. moreover, gp1 was identified by a polyclonal antiserum from an infect ... | 1992 | 1316948 |
| isolation of feline herpesvirus 1 from the trigeminal ganglia of acutely and chronically infected cats. | 1992 | 1316965 | |
| evolution of thymidine and thymidylate kinases: the possibility of independent capture of tk genes by different groups of viruses. | phylogenetic analysis of viral and cellular thymidine and thymidylate kinases was performed using computer-assisted methods. multiple alignments and tentative phylogenetic trees were generated for the two families of these enzymes, which include a) thymidine kinases (tk) of mammals, poxviruses, african swine fever virus, e. coli, and bacteriophage t4; and b) thymidylate kinases (thyk) of yeast and poxviruses and distantly related herpesvirus proteins with both enzymatic activities. analysis of t ... | 1992 | 1317076 |
| anterograde transport of hsv-1 and hsv-2 in the visual system. | the anterograde spread of herpesvirus in the visual system subsequent to retinitis has been observed clinically. we compared the ability of two well-studied herpes simplex virus (hsv) strains to be transported in the anterograde direction in the hamster visual system: strain mcintyre, representing hsv-1, and strain 186, representing hsv-2. intravitreal injection of hsv-2 labeled more retinorecipient neurons than did hsv-1, suggesting important type differences in the ability of hsv to infect ret ... | 1992 | 1317240 |
| the effects of equine rhinovirus, influenza virus and herpesvirus infection on tracheal clearance rate in horses. | the response of horses exposed to three common respiratory viruses was studied by measuring tracheal mucociliary clearance rates in the trachea. tracheal clearance rates (tcr) were determined before, during illness and following recovery in horses exposed to equine rhinovirus (erhv-2), equine influenza virus (eiv) and equine herpesvirus (ehv-4) by means of lateral scintigraphs made following an injection of technetium-99m sulphide colloid into the tracheal lumen. in six horses exposed to erhv-2, ... | 1992 | 1317244 |
| fatal disseminated infection with human herpesvirus-6. | a 13-month-old immunocompetent girl had fever, rash, and multisystem disease, and she eventually died of cardiac failure. autopsy revealed intracellular viral inclusions of the herpesvirus group, with results of in situ hybridization positive for human herpesvirus-6. this is apparently the first case of fatal disseminated herpesvirus-6 infection. | 1992 | 1317420 |
| transformation of large granular lymphocytic leukemia during the course of a reactivated human herpesvirus-6 infection. | a patient with cd3+ large granular lymphocytic (lgl) leukemia developed transformation (tf). the phenotype of the leukemic cells was cd3+, cd4+ and cd8-. the leukemic cell count increased rapidly; the cells became large and the nuclear outline, which had been reniform, became lobulated. anti-htlv-1 and anti-hiv antibodies were negative in the serum of the patient and no htlv-1 specific sequences were detected in the cdna of the leukemic cells by polymerase chain reaction (pcr). comparison of the ... | 1992 | 1317489 |
| rejection, herpesvirus infection, and ki-67 expression in endomyocardial biopsy specimens from heart transplant recipients. | we examined 164 endomyocardial biopsy specimens from 29 patients who received an orthotopic heart transplant since 1984. rejection was graded according to the hannover classification. non-isotopic in situ dna hybridization was conducted on cryostat sections with biotinylated probes for herpes simplex virus, epstein-barr virus, and cytomegalovirus. serial sections of 126 biopsies were investigated immunohistochemically for the presence of activated t lymphocytes and proliferating cells, with mono ... | 1992 | 1317559 |
| in vitro responses of cheetah mononuclear cells to feline herpesvirus-1 and cryptococcus neoformans. | in vitro t cell function by domestic cats and cheetahs to two common pathogens, feline herpesvirus-1 (fhv-1) and cryptococcus neoformans, was assessed. peripheral blood mononuclear cells (pbm) were stimulated with two strains of uv-inactivated fhv-1, whole heat-killed organisms or capsular antigen of cryptococcus neoformans, and proliferative responses measured. as a group, cheetah pbm responded significantly poorer than domestic cat pbm when cultured with fhv-1. however, individual cheetah resp ... | 1992 | 1317618 |
| expression of the marek's disease virus homolog of herpes simplex virus glycoprotein b in escherichia coli and its identification as b antigen. | marek's disease (md) is an oncogenic disease of chickens caused by md virus (mdv). among the major glycoproteins found in mdv-infected cells are gp100, gp60, and gp49, detected by immunoprecipitation and sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis with antisera previously shown to be reactive with b antigen in immunodiffusion analysis. following treatment with tunicamycin (tm), an inhibitor of n-linked glycosylation, the same sera were reported to detect two molecules call ... | 1992 | 1318409 |
| a serologic survey of the island fox (urocyon littoralis) on the channel islands, california. | the island fox is listed as a threatened species in california. a serologic survey of 194 island foxes (urocyon littoralis) was conducted over the entire range of the species on the channel islands (california, usa). antibody prevalence against canine adenovirus and canine parvovirus reached 97% and 59%, respectively, in some populations sampled. antibody prevalence of canine herpesvirus, canine coronavirus, leptospirosis and toxoplasmosis were low. antibodies against canine distemper virus were ... | 1992 | 1318424 |
| characterization of the dna of rodent herpesviruses by restriction endonuclease analysis and hybridization. | restriction endonuclease analysis, dot-blot hybridization, and dried gel hybridization were used to differentiate mouse cytomegalovirus, rat cytomegalovirus, and mouse herpesvirus strain 76. viral dna was obtained directly from virus-infected mouse or rat cells. restriction endonuclease digestion was performed by standard methods with bamhi, ecori, hindiii, and psti, and dna was analyzed by electrophoresis on agarose gels. cross-hybridization was used to determine the degree of genetic homology ... | 1992 | 1318445 |