Publications

TitleAbstractYear
Filter
PMID(sorted ascending)
Filter
characterization of two compatible small plasmids from sphingobium yanoikuyae.we isolated and characterized two small cryptic indigenous plasmids, pyan-1 (4,896 bp) and pyan-2 (4,687 bp), from sphingobium yanoikuyae, and developed a versatile system that permitted genetic manipulation of the genus sphingomonas. nucleotide sequencing of both plasmids revealed that they contained moba, mobs, and repa genes, which are predicted to encode proteins associated with mobilization and replication, in common. transformation with each plasmid harboring the antibiotic resistance gene ...200818391447
optimization of gellan gum production by sphingomonas paucimobilis atcc 31461 with nonionic surfactants using central composite design.the effect of nonionic surfactants on gellan production by sphingomonas paucimobilis was studied by the addition of 0.5, 0.75, 1.0, 1.25 and 1.5 g/l surfactants to shake flask culture. the nonionic surfactants tween 80, tween 40 and triton x-100 improved gellan production by s. paucimobilis, and the maximum yield (10.44 g/l) was obtained with triton x-100 at 0.75 g/l compared with that of the control fermentation (8.63 g/l) without surfactant. the do profiles associated with gellan production in ...200818397769
multi-factors on biodegradation kinetics of polycyclic aromatic hydrocarbons (pahs) by sphingomonas sp. a bacterial strain isolated from mangrove sediment.biodegradation of polycyclic aromatic hydrocarbons (pahs) in contaminated sediment is an attractive remediation technique and its success depends on biodegradation kinetics, and the optimal condition for the pah-degrading isolates; however, information on this aspect is still scarce. the effects of multi-factors on biodegradation of phenanthrene, a 3-ring model pah, in contaminated sediment slurry by sphingomonas sp. a bacterial strain isolated from surface mangrove sediment, were investigated u ...200818433800
[isolation, characterization and fermentation condition of coenzyme q10 producing strain with solanesol as precursor].a bacterium capable of producing coenzyme q10 (coq10, was isolated from soil. based on analysis of the 16s rdna sequence, traditional physiological characteristics, and biolog-gn, the strain was belonging to the genus sphingomonas and named as sphingomonas sp. zuteo3. the optimum fermentation condition of coq10 production was as following: glucose 15 g/l, (nh4)2so4 10 g/l, original ph 8.0 and at 25 degrees c. the addition of solanesol could improve coq10 production. the optimal condition for the ...200818437995
isolation and characterization of marine bacterial strain degrading fucoidan from korean undaria pinnatifida sporophylls.in spite of an increasing interest in fucoidans as biologically active compounds, no convenient commercial sources with fucoidanase activity are yet available. a marine bacterial strain that showed confluent growth on a minimal medium containing fucoidan, prepared from korean undaria pinnatifida sporophylls, as the sole carbon source was isolated and identified based on a 16s rdna sequence analysis as a strain of sphingomonas paucimobilis, and named sphingomonas paucimobilis pf-1. the strain dep ...200818467852
molecular cloning and characterization of cytochrome p450 and ferredoxin genes involved in bisphenol a degradation in sphingomonas bisphenolicum strain ao1.to clone and characterize the genes bisda and bisdb, encoding ferredoxin(bisd) (fd(bisd)) and cytochrome p450(bisd) (p450(bisd)), respectively, from the bisphenol a (bpa) degrading sphingomonas bisphenolicum strain ao1.200818492046
modified sublimation to isolate phenanthrene-degrading bacteria of the genera sphingomonas and burkholderia from xiamen oil port.sublimation was developed by alley and brown (2000) in order to isolate bacterial strains that were capable of degrading water insoluble compounds. in this study, sublimation was modified by the use of nutritional agar plates, instead of mineral salt agar, to isolate phenanthrene-degrading bacteria from a mixed culture that had been enriched under the selective pressure of high phenanthrene content. five strains were obtained with different morphology and degradation ability. based on the 16s rd ...200818502449
occurrence, production, and applications of gellan: current state and perspectives.bacterial exopolysaccharides (eps) are products of biotechnology that are of high interest due to their rheological properties. this is the case of sphingans, a group of structurally related eps secreted by members of the genus sphingomonas. among these, gellan is a multifunctional gelling agent produced in high yields by the non-pathogenic strain sphingomonas elodea atcc 31461. in its native form, gellan is a linear anionic eps based on a tetrasaccharide repeat unit composed of two molecules of ...200818506441
substrate recognition by family 7 alginate lyase from sphingomonas sp. a1.sphingomonas sp. a1 alginate lyase a1-ii', a member of polysaccharide lyase family 7, shows a broad substrate specificity acting on poly alpha-l-guluronate (poly(g)), poly beta-d-mannuronate (poly(m)) and the heteropolymer (poly(mg)) in alginate molecules. a1-ii' with a glove-like beta-sandwich as a basic scaffold forms a cleft covered with two lid loops (l1 and l2). here, we demonstrate the loop flexibility for substrate binding and structural determinants for broad substrate recognition and ca ...200818514736
interactions among buffelgrass, phenanthrene and phenanthrene-degrading bacteria in gnotobiotic microcosms.an experiment was undertaken in gnotobiotic microcosms to determine the role of buffelgrass (cenchrus ciliaris) and a phenanthrene-degrading bacterium (strain pm600) in the degradation of phenanthrene. the gram-negative bacterium was identified as a sphingomonas sp. by 16s rrna gene sequence analysis and as s. paucimobilis by biochemical tests (api 20 ne strips). its yellow pigment corresponded to nostoxanthin and its cellular fatty acids were typical of the genus sphingomonas. moreover, it was ...200818569317
microbial diversity of soil bacteria in agricultural field contaminated with heavy metals.in this study we evaluated the bacterial diversity in a soil sample from a site next to a chemical industrial factory previously contaminated with heavy metals. analysis of 16s rdna sequences amplified from dna directly extracted from the soil revealed 17 different bacterial types (genera and/or species). they included polyangium spp., sphingomonas spp., variovorax spp., hafina spp., clostridia, acidobacteria, the enterics and some uncultured strains. microbes able to tolerate high concentration ...200818595405
factors influencing the electrokinetic dispersion of pah-degrading bacteria in a laboratory model aquifer.despite growing interest in the electro-bioremediation of contaminated soil it is still largely unknown to which degree weak electric fields influence the fate of contaminant-degrading microorganisms in the sub-surface. here we evaluate the factors influencing the electrokinetic transport and deposition of fluorene-degrading sphingomonas sp. lb126 in a laboratory model aquifer exposed to a direct current (dc) electric field (1 v cm(-1)) typically used in electro-bioremediation measures. the infl ...200818607588
treatment of bromoamine acid wastewater using combined process of micro-electrolysis and biological aerobic filter.the wastewater originated from the production of bromoamine acid was treated in a sequential system of micro-electrolysis (me) and biological aerobic filter (baf). decolorization and cod(cr) removal rate of the proposed system was investigated with full consideration of the influence of two major controlling factors such as organic loading rate (olr) and hydraulic retention time (hrt). the removal rate of cod(cr) was 81.2% and that of chrominance could be up to 96.6% at an olr of 0.56 kg m(-3)d( ...200918621476
molecular characterization of the bacterial communities in the different compartments of a full-scale reverse-osmosis water purification plant.the origin, structure, and composition of biofilms in various compartments of an industrial full-scale reverse-osmosis (ro) membrane water purification plant were analyzed by molecular biological methods. samples were taken when the ro installation suffered from a substantial pressure drop and decreased production. the bacterial community of the ro membrane biofilm was clearly different from the bacterial community present at other locations in the ro plant, indicating the development of a speci ...200818621875
kinetics of chromate reduction during naphthalene degradation in a mixed culture.a mixed culture of bacillus sp. k1 and sphingomonas paucimobilis epa 505 was exposed to chromate and naphthalene. batch experiments showed that chromate was reduced and naphthalene was degraded by the mixed culture. chromate reduction occurred initially at a high rate followed by a decrease in rate until chromate reduction ceased. chromate reduction decreased in the mixed culture when a lower ratio of s. paucimobilis epa 505 to bacillus sp. k1 was utilized. a kinetic model incoporating a term fo ...199618629905
a glycan shield for bacterial sphingolipids.in this issue of chemistry & biology, kinjo et al. (2008) propose that the addition of oligosaccharides to the core outer membrane glycosphingolipid in sphingomonas spp. may be an adaptation that allows bacteria to evade recognition by natural killer t cells, thus suggesting a remarkable process of host/pathogen coevolution.200818634999
natural sphingomonas glycolipids vary greatly in their ability to activate natural killer t cells.mouse natural killer t (nkt) cells expressing an invariant t cell antigen receptor (tcr) recognize glycosphingolipids (gsls) from sphingomonas bacteria. the synthetic antigens previously tested, however, were designed to closely resemble the potent synthetic agonist alpha-galactosyl ceramide (alphagalcer), which contains a monosaccharide and a c18:0 sphingosine lipid. some sphingomonas bacteria, however, also have oligosaccharide-containing gsls, and they normally synthesize several gsls with di ...200818635002
[recognition and analysis on primary metabolites of nonylphenol].in order to discuss the nonylphenol (np) biodegradability effect in the wastewater treatment process, the primary metabolites and route of np had been analyzed in the molecular level by the interpretation of gas chromatography mass spectrometry (gc-ms) and the two experimental methods. a method was that activated sludge degraded two types of simulating sewage comparatively. type-1 wastewater was made up of water, glucose, peptone and inorganic salts, and type-2 sewage was mixed up of np and type ...200818637365
cloning and overexpression of alkaline phosphatase phok from sphingomonas sp. strain bsar-1 for bioprecipitation of uranium from alkaline solutions.cells of sphingomonas sp. strain bsar-1 constitutively expressed an alkaline phosphatase, which was also secreted in the extracellular medium. a null mutant lacking this alkaline phosphatase activity was isolated by tn5 random mutagenesis. the corresponding gene, designated phok, was cloned and overexpressed in escherichia coli strain bl21(de3). the resultant e. coli strain ek4 overexpressed cellular activity 55 times higher and secreted extracellular phok activity 13 times higher than did bsar- ...200818641147
characterization of the gut bacterial community in manduca sexta and effect of antibiotics on bacterial diversity and nematode reproduction.the tobacco hornworm, manduca sexta, is a model lepidopteran insect used to study the pathogenic and mutualistic phases of entomopathogenic nematodes (epns) and their bacterial symbionts. while intestinal microbial communities could potentially compete with the epn and its bacterial partner for nutrient resources of the insect, the microbial gut community had not been characterized previously. here, we show that the midgut of m. sexta raised on an artificial diet contained mostly gram-positive c ...200818647359
population dynamics in bioaugmented membrane bioreactor for treatment of bromoamine acid wastewater.the performances and microbial population changes in laboratory-scale membrane bioreactor (mbr) augmented with sphingomonas xenophaga qyy were investigated in the present study. it was demonstrated that after 30 days acclimation, the non-augmented mbr system were able to degrade bromoamine acid (baa) well. however, the efficiency of the system decreased with baa concentration increasing. while the augmented mbr showed higher capability, in which the color and cod removal were more than 90% and 5 ...200918650081
cloning and characterization of phosphoglucomutase and phosphomannomutase derived from sphingomonas chungbukensis dj77.the enzymes phosphoglucomutase (pgm) and phosphomannomutase (pmm) play an important role in the synthesis of extracellular polysaccharide. by colony hybridization of the fosmid library of sphingomonas chungbukensis dj77, an open reading frame (orf-1) of 1,626 nucleotides, whose predicted product is highly homologous with other pgm proteins from several bacterial species, was identified. an additional open reading frame (orf-2) of 1,437 nucleotides was identified, and its encoded protein shows a ...200818664520
assessment of inhibitory effects of fluoride-coated tubes on biofilm formation by using the in vitro dental unit waterline biofilm model.this study aimed to establish an in vitro model to simulate biofilms formed in dental unit waterlines (duwls) and to investigate the ability of polyvinylidene fluoride (pvdf)-coated tubes to inhibit biofilm formation using this model. the water and biofilm samples were obtained from duwls which had been clinically used for 2.5 years, and the predominant bacteria were identified. a conventional polyurethane tube was incubated for 24 to 96 h in the mixed flora of isolated bacteria, and the optimal ...200818676694
isomer-specific biodegradation of methylphenanthrenes by soil bacteria.it is assumed that bacteria generally degrade 2-methylphenanthrene (2mphe) in preference to 1-methylphenanthrene (1mphe), and that environmental biodegradation of methylated pahs therefore can be described qualitatively by changes in relative concentrations of these isomers. our objective was to investigate whether microbial phenanthrene degraders (sphingomonas and mycobacterium) show such isomer-specific pah degradation. eleven out of twenty-nine phenanthrene degraders could grow on methylphena ...200818678007
novel methylotrophic bacteria isolated from the river thames (london, uk).enrichment and elective culture for methylotrophs from sediment of the river thames in central london yielded a diversity of pure cultures representing several genera of gram-negative and gram-positive bacteria, which were mainly of organisms not generally regarded as typically methylotrophic. substrates leading to successful isolations included methanol, monomethylamine, dimethylamine, trimethylamine, methanesulfonate and dimethylsulfone. several isolates were studied in detail and shown by the ...200818681896
the screening, characterization, and use of omega-laurolactam hydrolase: a new enzymatic synthesis of 12-aminolauric acid.several omega-laurolactam degrading microorganisms were isolated from soil samples. these strains were capable of growing in a medium containing omega-laurolactam as sole source of carbon and nitrogen. among them, five strains (t7, t31, u124, u224, and u238) were identified as cupriavidus sp. t7, acidovorax sp. t31, cupriavidus sp. u124, rhodococcus sp. u224, and sphingomonas sp. u238, respectively. the omega-laurolactam hydrolyzing enzyme from rhodococcus sp. u224 was purified to homogeneity, a ...200818685217
enrichment and characterization of chlorinated organophosphate ester-degrading mixed bacterial cultures.chlorinated organophosphate ester (ope)-degrading enrichment cultures were obtained using tris(2-chloroethyl) phosphate (tcep) or tris(1,3-dichloro-2-propyl) phosphate (tdcpp) as the sole phosphorus source. in cultures with 46 environmental samples, significant tcep and tdcpp degradation was observed in 10 and 3 cultures, respectively, and successive subcultivation markedly increased their degradation rates. 67e and 45d stable enrichment cultures obtained with tcep and tdcpp, respectively, compl ...200818691527
sphingomonas paucimobilis peritonitis: a case report and literature review. 200818708553
biodegradation of dimethyl phthalate by sphingomonas sp. isolated from phthalic-acid-degrading aerobic granules.phthalic acid esters (paes) contamination in water, air, and soil is one of the major environmental concerns in many countries. besides the pae biodegradation process, the pae degrading bacteria have become one of the focuses of study. this study reports the successful isolation of one kind of indigenous bacterium pa-02 from phthalic acid (pa)-degrading aerobic granules. based on its 16s ribosomal dna sequence, isolate pa-02 was identified as sphingomonas genus with 100% similarity to sphingomon ...200818751698
cloning of poly(aspartic acid) (paa) hydrolase-1 gene from pedobacter sp. kp-2 and hydrolysis of thermally synthesized paa by its gene product.pedobacter sp. kp-2 can degrade and metabolize thermally synthesized alpha,beta-poly(d,l-aspartic acid) (tpaa), which contains 70% of unnatural beta-amide units, with high-molecular-weight. in this study, gene cloning and molecular characterization of paa hydrolase-1 from kp-2 was carried out. gene analysis reveals that deduced amino acid sequence of the enzyme shows a similarity to only that of paa hydrolase-1 from sphingomonas sp. kt-1. gpc and nmr analyses of the hydrolyzed products of tpaa b ...200918756460
metabolism of the explosive hexahydro-1,3,5-trinitro-1,3,5-triazine (rdx) in a contaminated vadose zone.the aim of this study was to explore biodegradation potential of hexahydro-1,3,5-trinitro-1,3,5-triazine (rdx) in a deep contaminated unsaturated zone over israel's coastal aquifer. while anaerobic biodegradation potential was observed throughout the profile down to the water table at a depth of 45m, aerobic biodegradation was limited to the surface of the unsaturated zone. traces of nitroso-rdx intermediates were detected in the soil samples, indicating possible in situ activity. polymerase cha ...200818774159
metabolism of nitrodiphenyl ether herbicides by dioxin-degrading bacterium sphingomonas wittichii rw1.nitrodiphenyl ether herbicides, including chlomethoxyfen, nitrofen, and oxyfluorfen are potent herbicides. some metabolites and parent compounds are considered as possible mutagens and endocrine disruptors. both properties pose serious hygienic and environmental risks. sphingomonas wittichii rw1 is a well-known degrader of polychlorinated dibenzo- p-dioxins, dibenzofurans, and diphenyl ethers. however, no detailed research of its metabolic activity has been performed against pesticides with a di ...200818778066
sphingomonas paucimobilis associated with localised calf myositis. 200818796597
a putative lipoprotein of sphingomonas sp. strain a1 binds alginate rather than a lipid moiety.gram-negative sphingomonas sp. strain a1 accumulates alginate in the cell surface pit and directly incorporates the polysaccharide into its cytoplasm through a 'superchannel'. a cell surface protein algp7 (27 kda) is inducibly expressed in the presence of alginate. although the protein algp7 was initially classified as a lipoprotein based on its primary structure, algp7 purified from strain a1 cells did not possess a lipid moiety. algp7 bound alginate efficiently at a neutral ph with a k(d) of 3 ...200818803672
[microbial biosynthesis and biotransformation of indigo and indigo-like pigments].indigo and indigo-like pigments are widely used in the industry of textile, food and medicine. now people pays more and more attention to developing an alternative method of indigo production which is "environment-friendy", especially microbial biosynthesis of indigo. many microorganisms involved in the biosynthesis of indigo have been isolated and characterized, and monooxygenase and dioxygenase have been identified to catalyze indigo biosynthesis. some genes encoding for these enzymes have bee ...200818807970
clinical features and treatment outcomes of infections caused by sphingomonas paucimobilis. 200818808348
treatment of pahs in contaminated soil by extraction with aqueous dna followed by biodegradation with a pure culture of sphingomonas sp.the biodegradation of polycyclic aromatic hydrocarbons (pahs) in aqueous deoxyribonucleic acid (dna) solution from contaminated soil washing was investigated. initial data with a model effluent consisting of anthracene, phenanthrene, pyrene and benzo[a]pyrene that were individually dissolved in 1% aqueous dna solution confirmed their positive degradation by sphingomonas sp. at around 10(8)cfuml(-1) initial cell loading. for anthracene and phenanthrene, complete removal was achieved within 1h tre ...200818814900
acclimated biomass that degrades sulfonated naphthalene formaldehyde condensate.a number of aerobic species were isolated from textile industry activated sludge wastewater. the bacterial consortium was acclimated during seven days before testing its capacity of sulfonated naphthalene-formaldehyde condensate (snfc) recalcitrant compound degradation. snfc's degradation was evaluated by using different techniques including: vapour pressure osmometry, spectroscopy uv-visible and chemical oxygen demand (cod). the degradation of snfc by acclimated bacterial consortium was determi ...200818819646
protein engineering of improved prolyl endopeptidases for celiac sprue therapy.due to their unique ability to cleave immunotoxic gluten peptides endoproteolytically, prolyl endopeptidases (peps) are attractive oral therapeutic candidates for protecting celiac sprue patients from the toxic effects of dietary gluten. enhancing the activity and stability of peps under gastric conditions (low ph, high pepsin concentration) is a challenge for protein engineers. using a combination of sequence- and structure-based approaches together with machine learning algorithms, we have ide ...200818836204
[relationship between soil fungistasis and bacterial community structure].as a natural attribute of clean and healthy soil, fungistasis is an important indicator of soil quality, and has positive ecological significance to the inhibition of plant disease eruption caused by soil-borne fungal pathogens. in this study, soil samples (0-15 cm) were collected from the abandoned land at shenyang experimental station of ecology, chinese academy of sciences, to which pesticides and fertilizers have never been applied for nearly 10 years. a series of soil samples with gradient ...200818839921
liposomal glycosphingolipids activate natural killer t cell-mediated immune responses through the endosomal pathway.natural killer t (nkt) cells recognize lipid antigens, such as glycosphingolipids (gsls), via cd1d and contribute to host defense against various pathogens. here, we demonstrate that gsls isolated from sphingomonas bacteria and inserted into liposomes (gsl-liposomes) enhance the activation of nkt cells and dendritic cells (dcs). gsl-liposomes remarkably enhanced the production of ifn-gamma from splenocytes in vitro and this enhancement depended on the content of the ph-sensitive lipid dioleoyl-p ...200918930085
evaluation of the airborne bacterial population in the periodically confined antarctic base concordia.the environmental airborne bacterial population in relation to human confinement was investigated over a period of 1 year in the concordia research station, which is located on the eastern antarctic plateau. the unique location of the station makes it suitable for different research domains such as glaciology, atmospheric sciences, astronomy, etc. furthermore, it is used as a test bed for long-duration spaceflights to study the physiologic and psychological adaptation to isolated environments. a ...200918972149
regulatory roles for nkt cell ligands in environmentally induced autoimmunity.the development of autoimmune diseases is frequently linked to exposure to environmental factors such as chemicals, drugs, or infections. in the experimental model of metal-induced autoimmunity, administration of subtoxic doses of mercury (a common environmental pollutant) to genetically susceptible mice induces an autoimmune syndrome with rapid anti-nucleolar ab production and immune system activation. regulatory components of the innate immune system such as nkt cells and tlrs can also modulat ...200818981095
chemoenzymatic synthesis of chiral 4,4'-bipyridyls and their metal-organic frameworks.the first enantiopure 4,4'-bipyridyls, , , and have been prepared in four or five steps via bacterial dioxygenase-catalysed cis-dihydroxylation of 4-chloroquinoline and c-c coupling; ligands and are found to be effective building blocks for the preparation of chiral metal-organic frameworks as demonstrated with the rational synthesis of two pillared-grid structures [zn(2)(fumarate)(2)(l)], which exhibit interesting structural and dynamic aspects.200818997944
molecular characteristics of xenobiotic-degrading sphingomonads.the genus sphingomonas (sensu latu) belongs to the alpha-proteobacteria and comprises strictly aerobic chemoheterotrophic bacteria that are widespread in various aquatic and terrestrial environments. the members of this genus are often isolated and studied because of their ability to degrade recalcitrant natural and anthropogenic compounds, such as (substituted) biphenyl(s) and naphthalene(s), fluorene, (substituted) phenanthrene(s), pyrene, (chlorinated) diphenylether(s), (chlorinated) furan(s) ...200919002456
high diversity of cultivable heterotrophic bacteria in association with cyanobacterial water blooms.cyanobacterial mass occurrences (water blooms) cause ecological, economic and health problems worldwide. still, little is known about heterotrophic bacteria associated with cyanobacteria and the interactions between those organisms. we isolated 460 bacterial strains from more than 40 lakes and rivers (151 samples), baltic sea (32 samples) and treated drinking water of seven treatment plants (29 samples). the water bodies and the raw water of the treatment plants were frequently dominated by high ...200919020559
complementary cooperation between two syntrophic bacteria in pesticide degradation.interactions between microbial species, including competition and mutualism, influence the abundance and distribution of the related species. for example, metabolic cooperation among multiple bacteria plays a major role in the maintenance of consortia. this study aims to clarify how two bacterial species coexist in a syntrophic association involving the degradation of the pesticide fenitrothion. to elucidate essential mechanisms for maintaining a syntrophic association, we employed a mathematica ...200919038271
microbial transformation of benzothiophenes, with carbazole as the auxiliary substrate, by sphingomonas sp. strain xldn2-5.benzothiophenes are a toxic and relatively recalcitrant fraction of coal-tar creosote. we investigated the co-metabolic transformation of benzothiophene (bt) and its derivatives by the carbazole (ca) degrader sphingomonas sp. xldn2-5, which is not able to grow on benzothiophenes as the sole carbon source. among the benzothiophenes tested, bt, 2-methylbenzothiophene (2-mbt) and 5-methylbenzothiophene (5-mbt) were co-metabolically converted. for 3-methylbenzothiophene, there was complete inhibitio ...200819047748
[degradation characteristics of bromoamine acid by sphingomonas sp. fl].a bacterial strain that could degrade bromoamine acid (baa) as the sole carbon source was isolated. it was identified as sphingomonas sp. based on 16s rrna gene sequence analysis and physio-biochemical characteristics. under the optimal growth conditions, with temperature of 30 degrees c, ph of 7.0, rotating rate of 100 r/min and (nh4)2so4 as the nitrogen source, the decolorization percentage of baa (100 mg/l) could reach 99% within 14 h. nacl of low concentration ( < 2%) facilitated the decolor ...200819068653
organization of three rrna (rrn) operons from sphingobium chungbukense dj77.the nucleotide sequences of all three rrna operons (rrna, rrnb, and rrnc) of sphingobium chungbukense dj77 were determined. the three rrn operons have the same gene order (16s rrna-trnaile-trnaala-23s rrna-5s rrna-trnafmet). the nucleotide sequences were identical over a 5,468 bp region spanning the 16s rrna gene to the 5s rrna gene. variability was observed in the 5s rrna-trnafmet spacer sequence of rrnb. the trnafmet gene sequences were identical except for two bases (t5794 and a5871 in rrnb, ...200819107400
sphingomonas paucimobilis bloodstream infections associated with contaminated intravenous fentanyl.nationally distributed medications from compounding pharmacies, which typically adhere to less stringent quality-control standards than pharmaceutical manufacturers, can lead to multistate outbreaks. we investigated a cluster of 6 patients in a maryland hospital who had sphingomonas paucimobilis bloodstream infections in november 2007. of the 6 case-patients, 5 (83%) had received intravenous fentanyl within 48 hours before bacteremia developed. cultures of unopened samples of fentanyl grew s. pa ...200919116043
sphingomonas mucosissima bacteremia in patient with sickle cell disease. 200919116079
biodegradation and detoxication of phenol by using free and immobilized cells of acinetobacter sp. xa05 and sphingomonas sp. fg03.strain xa05 and fg03 with high biodegradation activity of phenol were isolated from the activated sludge and phenol-contaminated soils in northwest of china, respectively. dna sequencing and homologous analysis of 16s rrna gene identified that xa05 belonged to an acinetobacter sp. and fg03 was closely related to the sphingomonas sp. cells of strain xa05 and fg03 were mixed at the ratio of 1:1, and polyvinyl alcohol (pva) was used as a gel matrix to immobilize mixed cells by repeated freezing and ...200919123092
degradation behaviors of nonylphenol ethoxylates by isolated bacteria using improved isolation method.nonylphenol ethoxylate (npeo)-degrading bacteria were isolated from activated sludge using an improved isolation method, and the corresponding degradation behaviours were investigated. eight npeo-degrading strains distributed in genera pseudomonas, sphingomonas, sphingobium, cupriavidus, ralstonia, achromobacter, and staphylococcus were acquired. the latter five genera have never been reported for the degradation of npeos. four degradation patterns were observed for the eight pure strains. in pa ...200819143306
[variation of bacterial community composition in the outbreak and decline of microcystis spp. bloom in lake xuanwu].water samples were collected in the outbreak and decline of microcystis spp. bloom in three different lake areas of lake xuanwu for studying the bacterial community composition during the bloom to provide clew for understanding the dynamics of water bloom. traditional microbiological methods were used for culturable bacterial analysis, while the amplified products of variable v3 region of bacterial 16s rdna were used for bacterial community dna finger print by using denaturing gradient gel elect ...200819143401
[a rare cause of nosocomial bacteremia: sphingomonas paucimobilis].sphingomonas paucimobilis, is a yellow-pigmented, aerobic, non-fermentative, non-spore-forming, gram-negative bacillus. infections by s. paucimobilis which is widely found in nature and hospital environments are rarely serious or life threatening. in this report we present a case of hospital acquired bloodstream infection due to s. paucimobilis. the patient had a history of hydrocephalus diagnosed at sixth months of his birth and had experienced two ventriculoperitoneal shunt surgery. he was hos ...200819149092
[risk assessment for environmental release of genetically engineered microorganism m-cds-1].to assess the environmental release risk of genetically engineered microorganism sphingomonas sp. m-cds-1.200819149163
glionitrin a, an antibiotic-antitumor metabolite derived from competitive interaction between abandoned mine microbes.the nutrient conditions present in abandoned coal mine drainages create an extreme environment where defensive and offensive microbial interactions could be critical for survival and fitness. coculture of a mine drainage-derived sphingomonas bacterial strain, kmk-001, and a mine drainage-derived aspergillus fumigatus fungal strain, kmc-901, resulted in isolation of a new diketopiperazine disulfide, glionitrin a (1). compound 1 was not detected in monoculture broths of kmk-001 or kmc-901. the str ...200919159274
characterization of a ring-hydroxylating dioxygenase from phenanthrene-degrading sphingomonas sp. strain lh128 able to oxidize benz[a]anthracene.sphingomonas sp. strain lh128 was isolated from a polycyclic aromatic hydrocarbon (pah)-contaminated soil using phenanthrene as the sole source of carbon and energy. a dioxygenase complex, phna1fa2f, encoding the alpha and beta subunit of a terminal dioxygenase responsible for the initial attack on pahs, was identified and isolated from this strain. phna1f showed 98%, 78%, and 78% identity to the alpha subunit of pah dioxygenase from novosphingobium aromaticivorans strain f199, sphingomonas sp. ...200919172265
alpha-glycosphingolipids via chelation-induced anomerization of o- and s-glucuronic and galacturonic acid derivatives.bacterial glycolipids containing either alpha-glucuronic acid or alpha-galacturonic acid residues have an important role in the innate-type immune response to gram-negative bacteria. synthesis of closely related compounds, including a novel alpha-so(2) glycolipid mimetic, is described from carbohydrate precursors where anomerization is a key step. very high stereoselectivites (>97:3 in favor of alpha) were observed from o-glycoside precursors.200919178158
an improved method to determine serine palmitoyltransferase activity.serine palmitoyltransferase (spt) catalyzes the condensation of l-serine and palmitoyl-coa, which is the rate-limiting step in the de novo synthesis of sphingolipids. spt activity is commonly measured by monitoring the incorporation of radiolabeled l-serine into 3-ketodihydrosphingosine. in this article, we introduce several adaptations of the established protocol to improve sensitivity, reproducibility, and practicability of the assay. a significant improvement of this new protocol is the possi ...200919181628
novel marine carbazole-degrading bacteria.eleven carbazole (car)-degrading bacterial strains were isolated from seawater collected off the coast of japan using two different media. seven isolates were shown to be most closely related to the genera erythrobacter, hyphomonas, sphingosinicella, caulobacter, and lysobacter. meanwhile, strains oc3, oc6s, oc9, and oc11s showed low similarity to known bacteria, the closest relative being kordiimonas gwangyangensis gw14-5 (90% similarity). southern hybridization analysis revealed that only five ...200919187209
novel modified version of nonphosphorylated sugar metabolism--an alternative l-rhamnose pathway of sphingomonas sp.several bacteria, including azotobacter vinelandii, possess an alternative pathway of l-rhamnose metabolism, which is different from the known bacterial pathway. in a previous article, a gene cluster related to this pathway was identified, consisting of the genes encoding the four metabolic enzymes l-rhamnose-1-dehydrogenase (lra1), l-rhamnono-gamma-lactonase (lra2), l-rhamnonate dehydratase (lra3) and l-2-keto-3-deoxyrhamnonate (l-kdr) aldolase (lra4), by which l-rhamnose is converted into pyru ...200919187228
isolation, phylogenetic analysis and screening of marine mollusc-associated bacteria for antimicrobial, hemolytic and surface activities.this study was undertaken to survey culturable heterotrophic bacteria associated with the marine ark shell anadara broughtoni inhabiting in the sea of japan, and to test isolates for their antimicrobial, hemolytic and surface activities with an emphasis on low-molecular-weight metabolites search. a total of 149 strains were isolated and identified phenotypically. a total of 27 strains were selected to be investigated phylogenetically by 165 rrna gene sequence analysis. the most bacteria were aff ...200819216104
brenneria salicis, the bacterium causing watermark disease in willow, resides as an endophyte in wood.brenneria salicis has been studied in willow wood only in relation to watermark disease. in this pathogenic condition, the bacterium occurs at high concentrations. pathogenicity of b. salicis is still uncontrollable and the disease unpredictable because the plant-bacteria interaction is not understood. thanks to molecular techniques b. salicis can be detected at low concentrations, which are found in most non-pathogenic interactions. brenneria salicis was identified and traced with a new specifi ...200919220404
enhanced production of coq10 by newly isolated sphingomonas sp. zuteo3 with a coupled fermentation-extraction process.the use of coenzyme q(10) (coq(10)) as a complementary therapy in heart failure will increase in proportion to the growth of the ageing population and the expansion of statins consumption. economical production of coq(10) by microbes will become more important due to the growing demands of the pharmaceutical industry. process simplification and integration might be one desirable pathway for economic production of coq(10) by microbial fermentation. in this report, the effect of a coupled fermenta ...200919221819
occurrence and diversity of nitrogen-fixing sphingomonas bacteria associated with rice plants grown in brazil.so far, the occurrence of nitrogen-fixing sphingomonas bacteria has been restricted to three strains of sphingomonas azotifigens. in this work, a group of 46 sphingomonas-like isolates, which originated from two rice varieties grown in two soils in brazil, were characterized based on morphological, physiological and genetic analyses. the pcr genus specifically applied indicated that all 46 isolates belonged to the sphingomonas genus and confirmed the results based on the yellow pigment of the co ...200919222576
water-borne sphingomonas paucimobilis epidemic in an intensive care unit. 200919232740
biodegradation of pharmaceutical and personal care products.medical treatments and personal hygiene lead to the steady release of pharmaceutical and personal care products (ppcps) into the environment. some of these ppcps have been shown to have detrimental environmental effects and could potentially impact human health. understanding the biological transformation of ppcps is essential for accurately determining their ultimate environmental fate, conducting accurate risk assessments, and improving ppcp removal. we summarize the current literature concern ...200919245937
bioremediation of cyanotoxins.cyanobacteria are a diverse group of mainly aquatic microorganisms which occur globally. eutrophication (nutrient enrichment) of water bodies, often as a result of human activities, results in prolific grow of cyanobacteria that develop into a thick scum or bloom. many of these blooms are toxic due to the production of hepatotoxins (microcystins and cylindrospermopsin) and/or neurotoxins (saxitoxins and anatoxins) posing a serious health hazard to humans and animals. the presence of these cyanot ...200919245938
arbuscular mycorrhiza and petroleum-degrading microorganisms enhance phytoremediation of petroleum-contaminated soil.while plants can phytoremediate soils that are contaminated with petroleum hydrocarbons, adding microbes to remediate contaminated sites with petroleum-degrading microorganisms and arbuscular mycorrhizal fungi (amf) is not well understood. the phytoremediation of arabian medium crude oil (aco) was done with a lolium multiflorum system inoculated with an amf (glomus intraradices) and a mixture of petroleum-degrading microorganisms--the bacterium, sphingomonas paucimobilis (sp) and the filamentous ...200819260211
the microorganism community of pentachlorophenol (pcp)-degrading coupled granules.coupled granules are self-immobilized aggregates of microorganisms under micro aerobic conditions, which have the dissolved oxygen (do) level of 0.6 mg.l(-1). the effects of do concentration on pentachlorophenol (pcp) reduction and its microbial community were investigated in a coupled anaerobic and aerobic reactor. both the diversity and the dynamicity of the eubacteria and archaea community, which were responsible for pcp degradation, were evaluated by means of amplification by polymerase chai ...200919273898
top-down systems biology modeling of host metabotype-microbiome associations in obese rodents.covariation in the structural composition of the gut microbiome and the spectroscopically derived metabolic phenotype (metabotype) of a rodent model for obesity were investigated using a range of multivariate statistical tools. urine and plasma samples from three strains of 10-week-old male zucker rats (obese (fa/fa, n=8), lean (fa/-, n=8) and lean (-/-, n=8)) were characterized via high-resolution 1h nmr spectroscopy, and in parallel, the fecal microbial composition was investigated using fluor ...200919275195
asymmetric dihydroxylation of aryl olefins by sequential enantioselective epoxidation and regioselective hydrolysis with tandem biocatalysts.chiral aryl vicinal diols were obtained in high ee and yield by asymmetric dihydroxylation of aryl olefins with tandem biocatalysts: one contains an enantioselective styrene monooxygenase, and the other contains a regioselective epoxide hydrolase.200919277363
microbial conversion of glucose to a novel chemical building block, 2-pyrone-4,6-dicarboxylic acid.2-pyrone-4,6-dicarboxylic acid (pdc) is a catabolic intermediate in sphingobium sp. syk-6 (previously characterized as sphingomonas paucimobilis syk-6), which is a degrader of lignin-derived aromatic compounds. recently, pdc has been also characterized as a novel starting material for several potentially useful synthetic polymers. in a previous study, we constructed a biosynthetic system in which pdc was generated efficiently from a chemically synthesized compound, protocatechuate. in order to d ...200919281857
exopolysaccharide production by lactose-hydrolyzing bacteria isolated from traditionally fermented milk.with increasing consumer demands for safer, healthier and more natural products, bacterially produced exopolysaccharides (epss) are becoming a viable option as an additive in designer-type foods. fresh milk samples from cattle and sheep were collected from informal settlements in south africa. after a three day incubation period at 25 degrees c, 550 bacterial strains were isolated and evaluated for eps production from lactose as sole carbon source. strains producing eps on lactose were identifie ...200919282044
detection of uvbr-sensitive and -tolerant bacteria in surface waters of the western north pacific.in order to evaluate the effects of solar ultraviolet radiation (uvr) on eubacterial community composition, we examined the tolerance of eubacterial phylotypes to solar uv radiation in surface waters of the western north pacific during september 2005. bromodeoxyuridine (brdu), a halogenated thymine analogue, was used for labeling newly synthesized dna in proliferating cells. thymine dimers (td), which are specifically formed in dna by biologically harmful ultraviolet b radiation (uvbr; 280-315nm ...200919285879
indigenous bacteria may interfere with the biocontrol of plant diseases.prodigiosin is a reddish antibiotic pigment that plays an important role in the biocontrol of plant diseases by the bacterium serratia marcescens. however, its activity is unstable under agricultural conditions; further, it can be degraded by various environmental factors. to examine the effect of epiphytic microbes on the stability of prodigiosin used for biological control processes, we collected a total of 1,280 bacterial isolates from the phylloplane of cyclamen and tomato plants. approximat ...200919288072
genetic and phenotypic diversity of fenitrothion-degrading bacteria isolated from soils.twenty-seven fenitrothion-degrading bacteria were isolated from different soils, and their genetic and phenotypic characteristics were investigated. analysis of the 16s rdna sequence showed that the isolates were related to members of the genera burkholderia, pseudomonas, sphingomonas, cupriavidus, corynebacterium, and arthrobacter. among the 27 isolates, 12 different chromosomal dna fingerprinting patterns were obtained by polymerase chain reaction (pcr) amplification of repetitive extragenic p ...200919307758
the most-probable-number enumeration of dichlobenil and 2,6-dichlorobenzamide (bam) degrading microbes in finnish aquifers.in groundwater subsurface deposits and a topsoil from five aquifers having 2,6-dichlorobenzamide (bam) in water, we determined the most-probable-number (mpn) of 2,6-dichlorobenzonitrile (dichlobenil) and metabolite bam degrading microorganisms. dichlobenil and bam were combined nitrogen sources in the mpn tubes, which were scored positive at concentrations <75% after 1 month incubation. aerobic and anaerobic microbes degrading dichlobenil and bam were common in samples in low numbers of 3.6-210 ...200919326247
sphingomonas sanxanigenens sp. nov., isolated from soil.strain nx02(t), a gram-negative, non-spore-forming, rod-shaped bacterium, was isolated from soil, and its taxonomic position was investigated using a polyphasic approach. chemotaxonomic analysis revealed that strain nx02(t) possessed q-10 as the predominant ubiquinone, sym-homospermidine as the major polyamine and c(18 : 1)omega7c, c(16 : 0) and c(14 : 0) 2-oh as the major fatty acids. the main polar lipids were sphingoglycolipid, phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol ...200919329595
cloning and characterization of phosphoglucose isomerase from sphingomonas chungbukensis dj77.phosphoglucose isomerase (pgi) is involved in synthesizing extracellular polysaccharide (eps). the gene encoding pgi in sphingomonas chungbukensis dj77 was cloned and expressed in e. coli, and the protein was characterized. the pgi gene from dj77 is 1,503 nucleotides long with 62% gc content and the deduced amino acid sequence shows strong homology with pgis from other sources. the molecular masses of pgi subunit and native form were estimated to be 50 kda and 97 kda, respectively. four potentia ...200919336005
vertical partitioning and expression of primary metabolic genes in a thermophilic microbial mat.a thermophilic microbial mat with a relatively simple morphological composition was used to study the expression of key metabolic genes between mat layers. mats comprised roseiflexus castenholzii, synechococcus sp., a sphingomonas-like proteobacterial taxon and an unidentified member of the thermotogae as determined by 16s rrna phylotypes. the diversity of expressed loci for key genes involved in oxygenic photosynthesis (cbbl), anoxygenic photosynthesis (pufm) and nitrogen fixation (nifh) was as ...200919347567
crystal structure of the glycosidase family 73 peptidoglycan hydrolase flgj.glycoside hydrolase (gh) categorized into family 73 plays an important role in degrading bacterial cell wall peptidoglycan. the flagellar protein flgj contains n- and c-terminal domains responsible for flagellar rod assembly and peptidoglycan hydrolysis, respectively. a member of family gh-73, the c-terminal domain (sph1045-c) of flgj from sphingomonas sp. strain a1 was expressed in escherichia coli, purified, and characterized. sph1045-c exhibited bacterial cell lytic activity most efficiently ...200919351587
static and dynamic sorption of phenanthrene in mangrove sediment slurry.the static and dynamic sorption of phenanthrene (phe) in three types of mangrove sediment slurries (sandy, silty and muddy) were described by three models, namely linear model, freundlich adsorption isotherm model and langmuir adsorption isotherm model. the freundlich adsorption isotherm was the best model to describe the static sorption behavior of phe in mangrove sediment slurry with the regression coefficients ranging from 0.96 to 0.99. in static sorption, the sorption capacity and sorption i ...200919356852
the external aldimine form of serine palmitoyltransferase: structural, kinetic, and spectroscopic analysis of the wild-type enzyme and hsan1 mutant mimics.sphingolipid biosynthesis begins with the condensation of l-serine and palmitoyl-coa catalyzed by the plp-dependent enzyme serine palmitoyltransferase (spt). mutations in human spt cause hereditary sensory autonomic neuropathy type 1, a disease characterized by loss of feeling in extremities and severe pain. the human enzyme is a membrane-bound hetereodimer, and the most common mutations are located in the enzymatically incompetent monomer, suggesting a "dominant" or regulatory effect. the molec ...200919376777
coaggregation by the freshwater bacterium sphingomonas natatoria alters dual-species biofilm formation.coaggregation is hypothesized to enhance freshwater biofilm development. to investigate this hypothesis, the ability of the coaggregating bacterium sphingomonas natatoria to form single- and dual-species biofilms was studied and compared to that of a naturally occurring spontaneous coaggregation-deficient variant. attachment assays using metabolically inactive cells were performed using epifluorescence and confocal laser scanning microscopy. under static and flowing conditions, coaggregating s. ...200919376917
sphingomonas japonica sp. nov., isolated from the marine crustacean paralithodes camtschatica.a sphingomonas-like bacterium, strain kc7(t), was isolated from a marine crustacean specimen obtained from the sea of japan and subjected to a polyphasic study. comparative 16s rrna gene sequence analysis positioned the novel strain in the genus sphingomonas as an independent lineage adjacent to a subclade containing sphingomonas trueperi lmg 2142(t), sphingomonas pituitosa ediv(t) and sphingomonas azotifigens nbrc 15497(t). strain kc7(t) shared highest 16s rrna gene sequence similarity (96.1 %) ...200919406815
crystallization and preliminary crystallographic analysis of the cell-surface alginate-binding protein algp7 isolated from sphingomonas sp. a1.sphingomonas sp. a1, a gram-negative bacterium, directly internalizes alginate macromolecules through a mouth-like pit that is present on its cell surface. the alginate-binding protein algp7, which was found to be localized on the cell surface, contributes to the accumulation of alginate in the pit. algp7 was crystallized at 293 k by means of the sitting-drop vapour-diffusion method with polyethylene glycol 3350 as a crystallizing agent. preliminary x-ray analysis showed that the algp7 crystal b ...200919407391
high yield synthesis of 12-aminolauric acid by "enzymatic transcrystallization" of omega-laurolactam using omega-laurolactam hydrolase from acidovorax sp. t31.the genes encoding omega-laurolactam hydrolases from cupriavidus sp. t7, acidovorax sp. t31, cupriavidus sp. u124, and sphingomonas sp. u238 were cloned and sequenced. nucleotide and amino acid sequence analysis of the four genes indicated that the primary structures of these omega-laurolactam hydrolases are significantly similar to the 6-aminohexanoate-cyclic-dimer hydrolase (ec 3.5.2.12). these genes were expressed in escherichia coli, and the omega-laurolactam hydrolysing activity of the reco ...200919420719
[cellulose-utilising bacterial association]. 200919449742
viability and potential for immigration of airborne bacteria from africa that reach high mountain lakes in europe.we have analysed the diversity of the bacteria, which grow after addition of concentrated airborne particles and desert dust in different microcosms combinations with water samples from oligotrophic alpine lakes. we used, on the one hand, airborne bacteria transported by an african dust plume and collected in a high mountain area in the central pyrenees (spain). on the other hand, we collected desert dust in mauritania (c. 3000 km distance, and a few days estimated airborne journey), a known sou ...200919453609
a novel aminopeptidase from burkholderia cepacia specific for acidic amino acids.an aminopeptidase specific for the n-terminal acidic residue (bcepap) was purified from the cell extract of burkholderia cepacia svr as a homotrimeric (subunit mass 66 kda) molecule. it was identified as an unassigned peptidase of family m61. the only other member characterized so far from this family is a broad-specificity aminopeptidase of sphingomonas capsulata (scapap) with preference for gly or ala residues. however, bcepap exhibited narrow specificity and the preferred substrate was a pept ...200919456865
polaromonas and hydrogenophaga species are the predominant bacteria cultured from granular activated carbon filters in water treatment.identification of the predominating cultivable bacteria in granular activated carbon (gac) filters used in a variety of water treatment plants for selecting representative strains to study the role of bacteria in the removal of dissolved organic matter.200919457026
azo dye decolorization by a new fungal isolate, penicillium sp. qq and fungal-bacterial cocultures.a new azo dyes-decolorizing fungi strain qq was isolated from activated sludge. it was identified as penicillium sp. based on 26s rrna gene sequence analysis. the study indicated that strain qq could aerobically decolorize reactive brilliant red x-3b by the way of bioadsorption, and nutrient-poor medium was more beneficial for adsorption. decolorization rate was inversely proportional to the size of mycelial pellets. the optimum ph was observed at 4 or 5 for x-3b decolorization. there was still ...200919473759
impact of bio-augmentation with sphingomonas sp. strain ttnp3 in membrane bioreactors degrading nonylphenol.this study evaluates the potential of bio-augmentation to improve the degradation of recalcitrant nonylphenol during the wastewater treatment in membrane bioreactors (mbr). one mbr containing activated sludge was bio-augmented using multistep inoculation with freeze dried sphingomonas sp. strain ttnp3, whereas a second control reactor contained activated sludge solely. the (14)c-labeled-nonylphenol isomer (4-[1-ethyl-1,3-dimethylpentyl]phenol) was applied as a single pulse. bio-augmentation resu ...200919495744
sphingomonas aestuarii sp. nov., isolated from tidal flat sediment.a novel bacterium (strain k4(t)) belonging to the genus sphingomonas was isolated from tidal flat sediment in korea. its morphology, physiology, biochemical features and 16s rrna gene sequence were characterized. colonies of this strain are yellow in colour and the cells are rod-shaped, exhibiting negative gram staining. the strain grows at 0-5 % (w/v) nacl and 20-35 degrees c, with optimal growth occurring at 0 % (w/v) nacl and 30 degrees c. phylogenetic analysis based on 16s rrna gene sequence ...200919502316
sphingomonas paucimobilis bacteraemia and septic arthritis in a diabetic patient presenting with septic pulmonary emboli.sphingomonas paucimobilis, a yellow-pigmented, aerobic, glucose non-fermenting, gram-negative bacillus, is a rare cause of human infection normally associated with immunocompromised hosts. we report a case of bacteraemia and septic arthritis in a 47-year-old diabetic man who presented with septic pulmonary emboli due to s. paucimobilis. the patient had an initial presentation of fever, right knee pain, coughing, dyspnoea and chest pain. the infection was treated successfully by surgical debridem ...200919528166
genetic characterization of microbial communities living at the surface of building stones.the aim of the present study was to reveal the microbial genetic diversity of epilithic biofilms using a dna-based procedure.200919552772
potentiation of murine innate immunity by alpha-galacturonosyl-type glycosphingolipids isolated from sphingomonas yanoikuyae and s. terrae.glycosphingolipids (gsls) are components of the outer membrane of sphingomonas species, commonly classified into two types, alpha-glucuronosyl ceramide (alpha-glcacer) and alpha-galacturonosyl ceramide (alpha-galacer), respectively. gsl-7 from s. yanoikuyae and gsl-13 from s. terrae, with alpha-galacer-type structure, possess dihydrosphingosine but with a different ratio of c21cyclopropane to c20:1, while other parts remain similar. we therefore examined if this difference in the ratio of c21cyc ...200919555191
Refresh
firstprev
12345678910...
nextlast
Displaying items 901 - 1000 of 1234