Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| antigenic relatedness between glycoproteins of human respiratory syncytial virus subgroups a and b: evaluation of the contributions of f and g glycoproteins to immunity. | the degree of antigenic relatedness between human respiratory syncytial virus (rsv) subgroups a and b was estimated from antibody responses induced in cotton rats by respiratory tract infection with rsv. glycoprotein-specific enzyme-linked immunosorbent assays of antibody responses induced by rsv infection demonstrated that the f glycoproteins of subgroups a and b were antigenically closely related (relatedness, r approximately 50%), whereas the g glycoproteins were only distantly related (r app ... | 1987 | 3305988 |
| application of pooled monoclonal antibodies for 1-hr detection of respiratory syncytial virus antigen in clinical specimens. | a fluorescein isothiocyanate-conjugated pool of monoclonal antibodies (moab) to respiratory syncytial virus (rsv) was prospectively evaluated for its utility as a direct, 1-hr test for the diagnosis of rsv infection. direct nasopharyngeal swab smears collected from 109 infants and children with acute respiratory illnesses were studied and compared with results obtained by indirect immunofluorescence using bovine polyclonal anti-rsv antibody on eluted cells derived from pooled nasopharyngeal and ... | 1987 | 3308304 |
| microplaque immunoperoxidase detection of infectious respiratory syncytial virus in the lungs of infected mice. | a rapid microplaque technique was developed for the detection of infectious respiratory syncytial virus (rsv) in the lungs of infected mice. infected lung homogenates were titrated on microwell hep-2 monolayers and incubated for 24 or 48 h. the microwells were then fixed with 4% formaldehyde in saline, or methanol containing 0.5% hydrogen peroxide. 24-h single cell infectious foci and 48-h microplaques were detected by an indirect immunoperoxidase (iip) assay using monoclonal antibodies specific ... | 1987 | 3312262 |
| comparison of nasopharyngeal washings and swab specimens for diagnosis of respiratory syncytial virus by eia, fat, and cell culture. | respiratory secretions for viral diagnosis are often collected with nasopharyngeal (np) swabs, although many laboratories recommend np aspirates or washings. we compared results using np washings and np swabs in three diagnostic rsv tests, a rapid rsv eia antigen test (abbott laboratories), an indirect fluorescent antibody test (fat) with rabbit antiserum, and virus culture (hep-2 cells). paired samples were collected from 121 children with suspected rsv bronchiolitis or pneumonia. a minitip swa ... | 1987 | 3322655 |
| comparison of two enzyme-linked immunosorbent assay (eia) kits with immunofluorescence and isolation in cell culture for detection of respiratory syncytial virus (rsv). | rapid diagnosis of respiratory syncytial virus (rsv) infections is based upon detection of viral antigen in cells obtained from the respiratory tract and usually employs immunofluorescence (if) reactions or enzyme-linked immunosorbent assays (eia). the pathfinder eia kit (kallestad diagnostics) was compared with the abbott eia kit by evaluating each against isolation of rsv in cell culture and detection of antigen by if. the pathfinder kit identified 116 of 129 culture-positive and 72 of 90 cult ... | 1987 | 3329591 |
| ribavirin aerosol treatment of serious respiratory syncytial virus infection in infants. | bronchiolitis is one of the most serious pulmonary infections commonly caused by respiratory syncytial virus (rsv). rsv disease occurs in yearly epidemics and is most severe in children 1 year of age or younger. approximately 1 in 50 to 1 in 100 infants are hospitalized after their first infection, and mortality fluctuates between 0.5 and 5.0 per cent. patients with underlying conditions such as congenital heart disease and bronchopulmonary dysplasia are at higher risk for morbidity and mortalit ... | 1987 | 3332796 |
| calcium requirement for syncytium formation in hep-2 cells by respiratory syncytial virus. | respiratory syncytial virus (rsv) grown in hep-2 cells in the absence of calcium did not induce cell fusion and syncytium formation. although the infected cells contained viral antigens, the cytopathic effect (giant cell formation) typical for rsv was not observed in calcium-free cultures. infectious virus yield was also slightly reduced (less than a one log10 reduction) in the absence of calcium. an analysis of viral proteins synthesized in both the presence and the absence of calcium revealed ... | 1988 | 3343306 |
| strain-specific serum antibody responses in infants undergoing primary infection with respiratory syncytial virus. | we sought to characterize the human respiratory syncytial virus (rsv) subgroup-specific antibody response to primary infection with rsv. rsv isolates from 43 infants and young children were typed as either subgroup a (long strain-like) or b (18537 strain-like) based on reactivity with monoclonal antibodies. acute-phase or preinfection and convalescent-phase sera were collected from the 43 subjects and tested in an enzyme-linked immunosorbent assay using purified f and g glycoproteins from rsv su ... | 1988 | 3346563 |
| prevention of nosocomial transmission of respiratory syncytial virus in a newborn nursery. | during three winter seasons prior to 1984-1985 the special care nursery at new england medical center experienced respiratory syncytial virus (rsv) epidemics that required closure of the unit. prior to and during the 1984-1985 winter season, several measures were taken to prevent recurrent nosocomial rsv transmission. in the winters of 1984-1985 and 1985-1986 there were 26 introductions of community-acquired rsv with no transmission of nosocomial cases during 1,688 patient days at risk as compar ... | 1988 | 3351266 |
| respiratory syncytial virus infection: a cause of respiratory distress syndrome and pneumonia in adults. | respiratory syncytial virus (rsv) infection, an important and sometimes lethal disease of infants and children, generally causes a milder and self-limited syndrome of cough, nasal congestion and fever in adults. while some evidence suggests that rsv may be responsible for more serious respiratory illness in the elderly and chronically ill, it has not been shown to cause life-threatening respiratory tract disease in previously healthy adults. this report describes a previously healthy woman who e ... | 1988 | 3354594 |
| modulation of systemic and mucosal immune responses to inhaled ragweed antigen in experimentally induced infection with respiratory syncytial virus implication in virally induced allergy. | groups of balb/c mice were either sham-infected or infected intranasally with respiratory syncytial virus (rsv). on the third day following intranasal inoculation, all groups of mice were exposed by inhalation to ragweed antigen for 5 consecutive days and rechallenged with ragweed on day 31. development of antibody activity to ragweed antigen was examined in serum and bronchial washings at regular intervals employing an elisa assay for igg and iga antibody activity and passive cutaneous anaphyla ... | 1988 | 3372039 |
| response of vaccinated and unvaccinated bighorn sheep (ovis canadensis canadensis) to experimental respiratory syncytial virus challenge. | five rocky mountain bighorn sheep (ovis canadensis canadensis), approximately 5 mo old and without detectable antibody titers to respiratory syncytial virus (rsv), were assigned to two groups to study the effects of rsv challenge inoculation in vaccinated (n = 3) and unvaccinated (n = 2) bighorns. the three lambs vaccinated with a modified live bovine rsv vaccine developed a detectable antibody response to the vaccine. vaccinated and unvaccinated lambs challenged with an ovine isolate of rsv dev ... | 1988 | 3373644 |
| effect of respiratory syncytial virus infection on the uptake of and immune response to other inhaled antigens. | groups of balb/c mice were sham infected or inoculated intranasally (in) with live rsv. from day 4 to 8 after infection, the animals were exposed in to ovalbumin (ova) with or without alum adjuvant. at different intervals, levels of ova concentration in serum, igg-anti-ova antibody activity in serum, and iga-anti-ova antibody activity in bronchial washings were determined, employing the elisa technique. ige-anti-ova antibody titers in serum and bronchial washings were assessed by pca. ova concen ... | 1988 | 3375265 |
| electron microscopic evidence for bridges between bovine respiratory syncytial virus particles. | electron microscopic examination of ultrathin sections of a continuous cell line of ovine kidney (ok) origin, infected by bovine respiratory syncytial virus (brsv), revealed the presence of well defined bridges between virus particles. this is the first report of this novel structure. observation of ultrathin sections of human rsv long strain also grown on ok cells did not show inter-particle bridges and therefore suggested that this structure could be specific to brsv. the biological significan ... | 1988 | 3385409 |
| risk of secondary bacterial infection in infants hospitalized with respiratory syncytial viral infection. | because infants hospitalized with respiratory syncytial virus (rsv) infection frequently receive antibiotics, our study was undertaken to determine what the actual risk of secondary bacterial infections in patients with rsv infection is and what effect antibiotic treatment might have on the course of illness. in a 9-year prospective study of 1706 children hospitalized with acute respiratory illnesses, 565 children had documented rsv infections. a subsequent bacterial infection rarely developed i ... | 1988 | 3397789 |
| selective activation of transcription by a novel ccaat binding factor. | a novel ccaat binding factor (cbf) composed of two different subunits has been extensively purified from rat liver. both subunits are needed for specific binding to dna. addition of this purified protein to nuclear extracts of nih 3t3 fibroblasts stimulates transcription from several promoters including the alpha 2(i) collagen, the alpha 1(i) collagen, the rous sarcoma virus long terminal repeat (rsv-ltr), and the adenovirus major late promoter. point mutations in the ccaat motif that show eithe ... | 1988 | 3399893 |
| [virus replication in a culture of smooth muscle cells from the human aorta]. | a new modification of the method for cultivation of cells from blood vessel explants has been proposed, and on its basis several cell lines from different vessels of human embryo and adult subjects have been derived. the sensitivity of vessel cells to viruses was studied on a model of aorta cell culture using a wide spectrum of rna and dna viruses. all the strains under study with the exception of respiratory-syncytial virus (rsv) and encephalomyocarditis (emc) virus induced a productive infecti ... | 1988 | 3414071 |
| [anti-tumor effect of hyperthermia plus hematoporphyrin derivative on malignant brain tumor]. | photoradiation therapy using the photosensitizing agent, hematoporphyrin derivative (hpd), and laser light has been currently employed as a new modality for the treatment of cancer. at present, however, the application of this therapy is limited to superficial tumors because of difficulties in achieving light penetration, whereas 13.56 mhz radiofrequency (rf) used in hyperthermia is capable to penetrate into deeper regions and elevate the temperature of brain tumors. in the present studies, the ... | 1988 | 3415865 |
| [studies on the possible association between severe neurological syndromes and compulsory vaccination in milano]. | from june 1978 throughout february 1979, 66 children with a mean age of 13 months were admitted to a pediatric intensive care unit in naples (santobono hospital) after a rapid onset of coma or deep stupor of unknown ethiology. the "mystery disease" ("il male oscuro") as somebody in naples has defined this syndrome, was suspected to be associated with tetanus and diphtheria immunization. epidemiological investigations and an international symposium of experts agreed not to consider the cases due ... | 1987 | 3435655 |
| intravenous immunoglobulin treatment of respiratory syncytial virus infections in infants and young children. | respiratory syncytial virus (rsv)-infected cotton rats (sigmadon hispidus) and owl monkeys (aotus trivirgatus) showed significant reductions in rsv shedding from their respiratory tracts following parenteral therapy with human intravenous immunoglobulin (ivig) containing high titers of rsv-neutralizing antibody. because this therapy was well tolerated and appeared safe, a double-blind, placebo-controlled ivig immunotherapy pilot study was performed on 35 hospitalized, rsv-infected infants and ch ... | 1987 | 3439796 |
| antitumour effect of interferon combined with hyperthermia against experimental brain tumour. | antiproliferative activity of recombinant murine interferon-beta (rec-muifn-beta) combined with hyperthermia against rous sarcoma virus-induced mouse malignant glioma (rsv glioma) was investigated both in vitro and in vivo. in vitro, the antiproliferative activity of rec-muifn-beta was enhanced by incubation at elevated temperatures (40 degrees and 43 degrees c). in vivo, combined therapy of rec-muifn-beta treatment and local tumour hyperthermia (43 degrees c) exerted a greater antitumour effect ... | 1987 | 3480323 |
| interleukin 1 and interleukin 1 inhibitor production by human macrophages exposed to influenza virus or respiratory syncytial virus. respiratory syncytial virus is a potent inducer of inhibitor activity. | respiratory viral infections are commonly associated with altered immune responses, such as proliferative responses to mitogens and antigens. to examine potential mechanisms, we examined production of il-1 and il-1 inhibitors by purified human peripheral blood-derived macrophages exposed to influenza virus or respiratory syncytial virus (rsv). il-1 and il-1 inhibitor activities in supernatant fluids from macrophages exposed to the viruses 24 h previously were measured using the standard mouse th ... | 1986 | 3485170 |
| cytotoxic t cell lysis of h-2-negative murine sarcoma cells. | the doh cell line was established from c3h.oh (h-2kd,dk) embryonic fibroblasts transformed with rous sarcoma virus (rsv) in vitro. when injected into syngeneic mice, doh cells were very weakly tumorigenic and induced a cytotoxic immune response. cytotoxic t lymphocytes (ctl) specifically lysed doh cells but not other rsv-induced sarcoma cells, which shared the h-2kd or h-2dk antigen, respectively, with doh. serological and immunochemical analysis of h-2 antigens subsequently showed that doh sarc ... | 1986 | 3486129 |
| passively acquired antibodies to respiratory syncytial virus impair the secondary cytotoxic t-cell response in the neonatal mouse. | passively acquired antibody has been known since the 1940s to impair the secondary antibody response to the homologous antigen. however, the effect of passive immunity on the t-cell response is largely unknown. the results presented here demonstrate that monoclonal antibodies (mabs) to respiratory syncytial virus (rsv), transferred in the mother's milk or injected directly, can reduce the generation of rsv-specific cytotoxic t-cell (tc) precursors by the neonatal mouse; the development of influe ... | 1986 | 3489669 |
| cytotoxic t cell specificity for respiratory syncytial virus proteins: fusion protein is an important target antigen. | we examined the specificity of balb/c cytotoxic t (tc) cells for respiratory syncytial virus (rsv) components, using recombinant vaccinia viruses (vv) coding for several individual rsv proteins. we found that immunization with the different vvs yielded the following tc memory cell populations: high levels of rsv-specific tc cells were induced with the fusion protein vv, but low levels were induced with vv coding for the rsv nucleoprotein. tc cell recognition of attachment glycoprotein, part of t ... | 1987 | 3497222 |
| lymphocyte transformation response of calves to respiratory syncytial virus. | virus-specific cell-mediated immunity, as determined by in vitro lymphocyte transformation (lt), was demonstrated in calves following infection and vaccination with respiratory syncytial virus (rsv). after experimental infection, 4 of 6 gnotobiotic calves and 6 of 21 conventional calves developed a significant lt response to rsv. by means of a whole blood assay, the lt responses of calves were examined after vaccination with an inactivated vaccine, which consisted of glutaraldehyde-fixed bovine ... | 1987 | 3498008 |
| in vitro and in vivo stimulation of murine lymphocytes by human respiratory syncytial virus strains. | respiratory syncytial virus (rsv) strains of subtype a (a2, wv9894, and wv12138) and of subtype b (wv1293, wv4843, and wv6873) are mitogenic in vitro for unprimed balb/c spleen cells. the virus also triggered splenocytes in vitro to secrete immunoglobulins. plaque-purified and uv-irradiated materials of both rsv subtypes produced comparable levels of dna synthesis. infectious materials of both subtypes also induced pronounced responses. lymphocyte activation with uv-inactivated rsv strain a2 was ... | 1987 | 3498209 |
| clearance of persistent respiratory syncytial virus infections in immunodeficient mice following transfer of primed t cells. | little is known of the role of t-cell mediated immune responses in the clearance and pathogenesis of respiratory syncytial virus (rsv) infection. in this study, we established persistent pulmonary rsv infections in athymic nu/nu balb/c mice or immunodeficient irradiated balb/c mice, and examined the patterns of virus clearance following adoptive transfer of splenic memory t cells. primed t cells transferred between day 5 and day 8 of infection will clear lung rsv from both nu/nu mice and irradia ... | 1987 | 3498683 |
| respiratory syncytial virus infections in sr croatia, yugoslavia. | the prevalence of respiratory syncytial virus (rsv) infection was studied during three consecutive annual outbreaks (1983-1986) in sr croatia, yugoslavia. a total of 1,238 subjects were examined, using rsv isolation and immunofluorescent (dtfa) methods, with 1,042 showing the signs of respiratory infection and 207 of these having a positive rsv finding. generally, the prevalence of mild upper respiratory infection (urti) was 18%, reaching a peak of 30% at 1 year of age. the prevalence of severe ... | 1987 | 3499590 |
| total and specific ige responses in the acute and recovery phases of respiratory infections in children. | total ige and specific ige antibodies to common allergens were determined by the enzyme-linked immunosorbent assay in children with mycoplasma pneumoniae or respiratory syncytial virus (rsv) infections. the decrease of total ige from the acute to convalescent phase was observed in 105 of 134 (78.4%) children with m. pneumoniae infection and in 40 of 49 (81.6%) of those with rsv infection. similarly, many patients showed a decrease of the responses of specific ige in the convalescent phase of the ... | 1987 | 3505529 |
| relation of serum antibody to glycoproteins of respiratory syncytial virus with immunity to infection in children. | immunity in relation to passively transferred maternal and naturally-induced serum antibody to the viral proteins was determined in 34 children who were followed from birth through three years of age for respiratory syncytial virus infection (rsv). sera were tested by immunoglobulin class-specific enzyme-linked immunosorbent assay using the attachment and fusion proteins of the long strain. the basis for immunity for maternal antibody in primary infection was assessed by a comparison of the dist ... | 1987 | 3509676 |
| common bacterial pneumonitis in infants. determining the etiology and tailoring the treatment. | in infantile pneumonia, we recommend close attention to the history and physical examination. baseline studies, including cbc, esr, blood cultures, and chest film, should be performed at onset and repeated as warranted. nasopharyngeal secretions or washings should be drawn by means of gentle suction and specimens sent for gram stain, fluorescent antibody stain for respiratory syncytial virus, and culture for bacteria and for viruses if possible. acute and convalescent serum specimens should be o ... | 1986 | 3511465 |
| detection of respiratory syncytial virus in nasopharyngeal secretions by a biotin-avidin elisa more sensitive than the fluorescent antibody technique. | the presence of respiratory syncytial virus (rsv) was investigated by immunofluorescent antibody (if) technique and by a biotin/avidin (ba) elisa in 156 samples of nasopharyngeal secretions (nps) obtained from infants and small children with acute respiratory disease. of 70 rsv-if-positive nps, 68 were positive by ba-elisa. of 86 rsv-if-negative nps, eight were positive by ba-elisa. ba-elisa could detect 0.5-1.0 ng rsv-protein. | 1986 | 3512768 |
| resistance to human respiratory syncytial virus (rsv) infection induced by immunization of cotton rats with a recombinant vaccinia virus expressing the rsv g glycoprotein. | a cdna copy of the g glycoprotein gene of human respiratory syncytial virus (rsv) was placed under control of a vaccinia virus promoter and inserted into the thymidine kinase locus of the vaccinia virus genome. the recombinant vaccinia virus retained infectivity and expressed a 93-kda protein that migrated with the authentic rsv g glycoprotein upon polyacrylamide gel electrophoresis. glycosylation of the expressed protein and transport to the cell surface were demonstrated in the absence of othe ... | 1986 | 3513191 |
| rapid detection of respiratory syncytial virus in nasopharyngeal aspirates by a commercial enzyme immunoassay. | a commercial enzyme immunoassay (eia) for the rapid detection of respiratory syncytial virus (rsv) in respiratory secretions was evaluated by comparison with both virus isolation in hep-2 cells and indirect immunofluorescence (ifa) staining of exfoliated respiratory cells. initial examination of 80 nasopharyngeal aspirates collected from infants with acute respiratory illness showed that the rsv eia was positive for 21 of 24 specimens positive by virus isolation or ifa (87.5% sensitivity) and ne ... | 1986 | 3514658 |
| evaluation of clinical specimens for the presence of respiratory syncytial virus antigens using an enzyme immunoassay. | an enzyme-linked immunoassay (eia) was developed for the detection of respiratory syncytial virus (rsv) antigen in nasopharyngeal secretions. this assay, which employs goat and rabbit anti-rsv as the capture and detector antibodies respectively, was used in a retrospective evaluation of frozen clinical specimens from children. the eia results were compared with those of virus isolation in cell culture and direct fluorescent antibody staining performed at the time of specimen collection. the sens ... | 1986 | 3517226 |
| detection of respiratory syncytial virus in nasopharyngeal secretions by enzyme-linked immunosorbent assay, indirect immunofluorescence, and virus isolation: a comparative study. | an enzyme-linked immunosorbent assay (elisa) was developed for the detection of respiratory syncytial virus (rsv) antigens in nasopharyngeal secretions (nps) from children with acute respiratory disease. antisera against rsv nucleocapsids were used as immunoreagents for this test system. the results obtained by rsv antigen elisa were compared to those of indirect immunofluorescence (if) and tissue culture virus isolation (tc). of the 404 nps obtained, 278 were tested in parallel by elisa and if ... | 1986 | 3522805 |
| use of egg-yolk antibody for detection of respiratory syncytial virus in nasal secretions by elisa. | egg-yolk immunoglobulins extracted from the eggs of hens immunized with respiratory syncytial virus (rsv) have been used as a reagent in double sandwich elisa for detecting rsv in nasal secretions. the sensitivity of virus detection was the same in indirect elisa, using rabbit anti chicken globulin conjugate, as when biotinylated yolk globulin and labeled avidin were used for detection. the specificity of elisa for detecting rsv using yolk antibody was similar to that achieved by indirect immuno ... | 1986 | 3524512 |
| value of rapid diagnosis of respiratory syncytial virus infection on management of small infants. | respiratory syncytial virus (rsv) is a common cause of infection in infancy and early childhood. a presumptive diagnosis of rsv infection can frequently be made on clinical grounds. confirmation can be made by viral culture, which may take 3 to 7 days. immunofluorescent assay (ifa) is a specific and sensitive test that can provide laboratory confirmation of rsv infection the same day. rapid diagnosis of rsv infection may have implications regarding prevention of nosocomial spread of rsv, early i ... | 1986 | 3524958 |
| normal and malignant cells, including neurons, deposit plasminogen activator on the growth substrata. | the results of four different assay methods showed that both normal and malignant plasminogen activator-secreting cells deposited substantial amounts of this protease on tissue-culture substrata, including collagen coatings. the cells studied were rous sarcoma virus (rsv)-transformed vole fibroblasts, a malignant neural cell line (ng108-15) capable of neurite formation, and normal mouse-regenerating sensory neurons. deposited plasminogen activator was detected by a fibrin overlay assay at sites ... | 1986 | 3527728 |
| expression of the f glycoprotein of respiratory syncytial virus by a recombinant vaccinia virus: comparison of the individual contributions of the f and g glycoproteins to host immunity. | a cdna clone representing the mrna coding sequence of the fusion glycoprotein (f) gene of human respiratory syncytial virus (rsv) was constructed and inserted into the thymidine kinase gene of vaccinia virus (wr strain) under the control of a vaccinia virus promoter. the resulting recombinant vaccinia virus, vaccinia f, expressed the f1 and f2 cleavage products (48 and 20 kda, respectively) of the f glycoprotein in cell culture. f1 and f2 were indistinguishable from their authentic rsv counterpa ... | 1986 | 3532115 |
| strategy for efficient detection of respiratory viruses in pediatric clinical specimens. | direct immunofluorescence (if) with a polyclonal respiratory syncytial virus (rsv)-specific antibody preparation was used for antigen detection during the 1982-1983 rsv season (155 specimens) and gave an overall sensitivity of 94% with 87% specificity compared with viral culture. indirect if was used in the 1983-1984 season (265 specimens) and exhibited sensitivity of 96% with specificity of 79%. during these two seasons, 42 of 224 (18.8%) specimens that were if-negative for rsv grew viruses oth ... | 1986 | 3536275 |
| comparison of two new tests for rapid diagnosis of respiratory syncytial virus infections by enzyme-linked immunosorbent assay and immunofluorescence techniques. | the sensitivity and the specificity of two new commercial reagent tests, an indirect fluorescent antibody test (fat) with a mouse monoclonal antibody (mab) against respiratory syncytial virus (rsv) and an enzyme-linked immunosorbent assay (elisa) rsv antigen detection kit, were determined by a comparison of results from these tests with those of tissue culture isolation and an indirect fat with bovine polyclonal antibody (bpa). of 251 nasal aspirates from infants with suspected rsv infection, po ... | 1986 | 3536993 |
| rapid diagnosis of respiratory syncytial virus infection by antigen immunofluorescence detection with monoclonal antibodies and immunoglobulin m immunofluorescence test. | during a respiratory syncytial virus (rsv) infection outbreak in a pediatric hospital, diagnosis was made by immunofluorescence on smears by using an anti-rsv monoclonal antibody (ifm). immunoglobulins m and g were titrated by indirect immunofluorescence on hep-2 cells infected with an rsv strain. the ifm was sensitive (89%) and specific (75%) when compared with the cell culture method. we showed that the specimens which were found positive by ifm and negative by cell culture were truly positive ... | 1986 | 3536995 |
| comparison of hep-2 cell culture and abbott respiratory syncytial virus enzyme immunoassay. | respiratory syncytial virus (rsv) is the leading cause of lower respiratory tract infection in children. rapid identification of rsv infections would allow for specific chemotherapy. we evaluated a means of rapid diagnosis, the abbott enzyme immunoassay (eia), by using 314 stored nasopharyngeal aspirates. rsv antigens were identified in 62 of 66 rsv culture-positive specimens. an additional 37 specimens from which rsv was not isolated were positive in the eia. of these, 29 were confirmed as trul ... | 1987 | 3546371 |
| critical evaluation of viral antigen data in paget's disease of bone. | this study evaluates previous viral antigen data obtained from fixed tissue sections and cells grown in culture from bone affected by paget's disease. finding antigens to both respiratory syncytial virus (rsv) and measles virus (mv) in the same osteoclasts of ten patients could not be explained on the basis of any previously known cross-reactivity. therefore, possible causes for these observations were sought. monoclonal antibodies to viral proteins of rsv and mv were used to label proteins. pol ... | 1987 | 3549088 |
| immunoglobulin administration and ribavirin therapy: efficacy in respiratory syncytial virus infection of the cotton rat. | we studied the effects of combined administration of human immunoglobulin (ivig) and ribavirin aerosol on respiratory syncytial virus (rsv) infection in cotton rats (sigmodon hispidus). cotton rats assigned to receive combined therapy were administered gamimune, a preparation of purified ivig with a high titer of anti-rsv neutralizing activity, intraperitoneally 24 h prior to intranasal rsv challenge and then treated with ribavirin aerosol 3 days after challenge. lung viral titers from these cot ... | 1987 | 3550674 |
| effectiveness of topically administered neutralizing antibodies in experimental immunotherapy of respiratory syncytial virus infection in cotton rats. | initial studies of the prophylactic effect of parenterally administered respiratory syncytial virus (rsv)-neutralizing antibodies in cotton rats indicated that virus replication in lung tissues was restricted when animals with preexisting antibody titers in serum of 1:100 or more (as measured by plaque reduction) were challenged intranasally with 10(4) pfu of virus. subsequently, a therapeutic effect of parenterally administered rsv antibodies (present in human gamma globulin) was demonstrated i ... | 1987 | 3553614 |
| aerosolized ribavirin treatment of respiratory syncytial virus infection in infants hospitalized during an epidemic. | thirty-three infants with predisposing conditions and/or severely symptomatic with respiratory syncytial virus (rsv) infection were treated with aerosolized ribavirin during a 12-week period at oklahoma children's memorial hospital. these patients were compared with 97 untreated patients with rsv infection hospitalized during the same epidemic. despite preconditions which selected for a more seriously ill treatment group, patients who received ribavirin showed prompter resolution of the illness ... | 1987 | 3562136 |
| neutralizing activity in human milk fractions against respiratory syncytial virus. | neutralizing activity against respiratory syncytial virus (rsv) was measured in milk samples from 17 healthy women whose infants had an acute infection with respiratory syncytial virus (rsv) and from 27 women with healthy infants. all milk samples were obtained 2-8 months post partum. neutralizing activity was detected in 36 samples. no major difference in neutralizing titers was observed between the two groups, and the titers were low. rsv-specific iga was found in two samples, and rsv-specific ... | 1986 | 3564936 |
| genetic resistance to fowl cholera is linked to the major histocompatibility complex. | chickens of the iowa state s1 line have been selected for ability to regress rous sarcoma virus-induced (rsv) tumors, humoral immune response to gat (ir-gat), and erythrocyte antigen b. sublines homozygous at the major histocompatibility complex (mhc), as well as f1 heterozygotes and f2 segregants, were tested for resistance to fowl cholera by challenge with pasteurella multocida strain x73. control of the response at high doses was associated in a preliminary study with ir-gat and response to r ... | 1987 | 3570380 |
| respiratory syncytial virus (rsv) infection rate in personnel caring for children with rsv infections. routine isolation procedure vs routine procedure supplemented by use of masks and goggles. | respiratory syncytial virus (rsv) infections in hospitalized children were identified by indirect fluorescent antibody technique. patients with rsv infections were assigned to one of two isolation categories. in one category, the health care workers entering the child's room did not wear masks and goggles; in the other category, the workers did wear masks and goggles. the rsv illness rate in health care workers using masks and goggles was 5%, but the rate for those not using masks and goggles wa ... | 1987 | 3578197 |
| prevention of nosocomial respiratory syncytial virus infections through compliance with glove and gown isolation precautions. | to determine whether increased compliance with a policy of glove and gown isolation precautions could reduce the high rate of nosocomial respiratory syncytial virus (rsv) infection on an infant and toddler ward, we conducted a longitudinal intervention trial during three rsv seasons, from 1982 to 1985, with an intervention to increase compliance introduced midway through the second season. the risk of acquiring rsv infection in the hospital was adjusted for the intensity of nosocomial exposure t ... | 1987 | 3600729 |
| identification of an amino terminal domain required for the transforming activity of the rous sarcoma virus src protein. | transformation of chicken cells by rous sarcoma virus (rsv) requires the functional expression of the viral src protein, a tyrosine protein kinase, pp60src. variants of rsv containing deletions within the amino terminal one-third of the src protein have been identified that exhibit either temperature-sensitive or transformation-defective phenotype when used to infect chicken embryo cells. to define the regions within the amino terminal portion of pp60src that influence morphological transformati ... | 1987 | 3660586 |
| vaccines for respiratory disease in cattle. | respiratory disease is one of the most serious disease complexes affecting beef cattle production. for example, it is claimed to cost the uk industry about 70 million pounds per year. it is usually associated with young cattle and can occur in a variety of situations. it is a good example of multifactorial disease in that its aetiology involves both infection by a variety of microorganisms and a number of environmental factors. several distinct syndromes occur and a number of microorganisms are ... | 1987 | 3673201 |
| growth-inhibitory protein present in rabbit serum, which is more effective on tumorigenic rat liver epithelial cells than on non-tumorigenic ones: its species, and mode of existence. | we have previously reported that in culture, rabbit serum inhibits the growth of the epithelial cell line from buffalo rat liver (brl) lower than that of the tumorigenic one transformed by rous sarcoma virus (rsv-brl). here, the serum was fractionated by several different methods. the findings are: 1) the growth inhibitor present (gi) existed as large complexes with non-inhibitory proteins; 2) the complexes were dissociated by 1 m nacl plus 6 m urea; 3) the dissociated gi did not pass through me ... | 1987 | 3689392 |
| comparison of washed nasopharyngeal cells and whole nasal secretions for detection of respiratory syncytial virus antigens by enzyme-linked immunosorbent assay. | we compared washed nasal epithelial cells with unfractionated nasal secretions as sources of respiratory syncytial virus (rsv) antigens in an indirect enzyme-linked immunosorbent assay (elisa). of 28 infants positive for rsv by virus isolation or direct immunofluorescence or both, 27 (96%) were positive by elisa with whole nasal secretions, whereas only 19 (68%) were positive by elisa with the matching washed-cell fractions. furthermore, the elisa absorbances obtained with nasal secretions were ... | 1986 | 3700621 |
| genetic aspects of antibody responses in chickens to different classes of antigens. | six breeding groups of chickens, each characterized by a different haplotype of the b blood group system, were challenged with different classes of antigens, namely newcastle disease vaccine (nd), infectious bronchitis vaccine (ib), infectious bursal disease viral agent (ibd), salmonella pullorum antigen (p), and sheep red blood cells (srbc). parents were challenged at 20 weeks of age, and their offspring were challenged at 3 weeks of age. blood samples were taken from the parents at 1 week afte ... | 1986 | 3703775 |
| [respiratory syncytial virus infections in newborn infants]. | twenty-three of 23 neonates were contaminated in the course of an outbreak of respiratory syncytial virus (rsv) in a neonatal care unit. symptoms among 22 infected symptomatic infants included rhinitis (n = 21), dyspnea (n = 19), cough (n = 17), apnea (n = 5), seizures (n = 3), fever (n = 3). five patients presented with severe respiratory distress. the occurrence of non-obstructive apnea was significantly correlated with a history of respiratory disease, rsv infection during the first 15 days o ... | 1986 | 3718165 |
| culture of respiratory syncytial virus infected diploid bovine nasal mucosa cells on cytodex 3 microcarriers. | in preliminary studies, a system for the propagation of respiratory syncytial virus (rsv) using cytodex 3 microcarriers was established. optimal growth conditions were defined in culture to be a microcarrier concentration of 1.5 g/l with a cell inoculum density of 4 x 10(4) cells/ml tissue culture medium. under these conditions growth coefficients were 3-fold greater in microcarriers when compared to conventional monolayer culture in roux culture flasks. maximum yield of virus antigen was achiev ... | 1986 | 3718237 |
| respiratory syncytial viral infection in children with compromised immune function. | for 10 winters, 608 children five years old or younger who were hospitalized with respiratory syncytial virus (rsv) infection were prospectively studied to evaluate the relation between their immune status and the severity of their infection. forty-seven had been immunocompromised by chemotherapy, steroid therapy, or a primary immunodeficiency disorder. among the immunocompromised children, those receiving chemotherapy for cancer and those with immunodeficiency disease had more severe rsv diseas ... | 1986 | 3724802 |
| respiratory syncytial virus: a report of a 5-year study at a children's hospital. | a study over a 5-year period (1979-1983) of rsv infections in children in sydney, australia is reported. in common with findings made elsewhere in the world, annual epidemics of rsv infection commencing in autumn and lasting 4-6 months, with peak activity in mid-winter, were observed in 1979, 1980, and 1983. however, in 1981 and 1982 virus activity was first detected in midsummer, peaked in autumn, and was present throughout most months of the year. the alteration in virus activity in 1981 and 1 ... | 1986 | 3746259 |
| serum and nasal-wash immunoglobulin g and a antibody response of infants and children to respiratory syncytial virus f and g glycoproteins following primary infection. | an enzyme-linked immunosorbent assay (elisa) with immunoaffinity-purified fusion (f) or attachment (g) glycoprotein was used to measure the serum and secretory immune responses of 18 infants and children, 4 to 21 months of age, who underwent primary infection with respiratory syncytial virus (rsv). most of the 10 older individuals (9 to 21 months of age) developed moderate levels of serum and nasal-wash immunoglobin a (iga) and igg f and g antibodies. these individuals developed a moderate level ... | 1986 | 3754878 |
| dissociation between serum neutralizing and glycoprotein antibody responses of infants and children who received inactivated respiratory syncytial virus vaccine. | the serum antibody response of infants and children immunized with formalin-inactivated respiratory syncytial virus (rsv) vaccine 20 years ago was determined by using an enzyme-linked immunosorbent assay specific for the rsv fusion (f) and large (g) glycoproteins and a neutralization assay. twenty-one young infants (2 to 6 months of age) developed a high titer of antibodies to the f glycoprotein but had a poor response to the g glycoprotein. fifteen older individuals (7 to 40 months of age) deve ... | 1986 | 3755730 |
| simplified enzyme-linked immunosorbent assay for specific antibodies to respiratory syncytial virus. | a simplified and reliable enzyme-linked immunosorbent assay (elisa) was applied to the detection of serum antibodies against respiratory syncytial virus (rsv). rsv-infected cells were fixed and dried on 96-well microtiter plates and kept at 4 degrees c. the titers of reference sera were determined by endpoint dilution. a linear relation was found between the titers and the logarithm of absorbance values of sera diluted to 1:1,000 (r = 0.93, p less than 0.001). measurement of rsv antibodies was d ... | 1986 | 3771748 |
| effect of age and preexisting antibody on serum antibody response of infants and children to the f and g glycoproteins during respiratory syncytial virus infection. | the serum antibody response of 50 infants and children infected with respiratory syncytial virus (rsv) was determined by a glycoprotein-specific enzyme-linked immunosorbent assay, and the effects of age and preexisting antibody titer at the time of rsv infection on response to the g and f glycoproteins of rsv were examined. the immune response to the g and f glycoproteins was assessed with anti-human immunoglobulin a to permit measurement of the response of young infants in the presence of mater ... | 1986 | 3771779 |
| predictive value of respiratory syncytial virus-specific ige responses for recurrent wheezing following bronchiolitis. | to determine whether the magnitude of the respiratory syncytial virus (rsv)-specific ige response at the time of an episode of rsv bronchiolitis in infancy accurately predicts the development of subsequent wheezing episodes, we observed 38 infants prospectively from the time of an episode of infantile bronchiolitis through 48 months of age. peak rsv-ige titers were measured at the time of the bronchiolitis episode using an elisa procedure. notation was made of both the number of subsequent wheez ... | 1986 | 3772657 |
| gianotti-crosti syndrome associated with infections other than hepatitis b. | although the gianotti-crosti syndrome (gcs) is regularly associated with hepatitis b infection elsewhere, in north america that association is rarely made. accordingly, we studied nine children with acral, symmetrical eruptions typical of gcs for evidence of other infections. all were negative for hepatitis b surface antigen. viral cultures were done in nine patients, and viruses isolated in two. one patient with a respiratory prodrome had respiratory syncytial virus (rsv) isolated, and a second ... | 1986 | 3773143 |
| the use of eye-nose goggles to control nosocomial respiratory syncytial virus infection. | we evaluated an eye-nose goggle to determine its usefulness in reducing nosocomial rsv infection in patients and staff members on our infant ward. during a community outbreak of rsv in 1984, infection was assessed by biweekly routine viral cultures on all ward personnel and patients and also by seroconversion in personnel. for three weeks staff members wore the goggles; two (5%) adults and one (6%) child acquired nosocomial infection. during the subsequent three-week study period, goggles were n ... | 1986 | 3773177 |
| age related igg subclass response to respiratory syncytial virus fusion protein in infected infants. | respiratory syncytial virus (rsv) fusion protein was purified by immunoaffinity chromatography using a mouse monoclonal antibody coupled to affi-gel 10. the fusion protein was homogeneous by sodium dodecyl sulfate polyacrylamide gel electrophoresis (sds-page) and free of other detectable viral or cellular protein. the purified fusion protein was used in a quantitative enzyme-linked immunosorbent assay (elisa) to measure the age-related antibody response to this protein in infected infants. the f ... | 1986 | 3791688 |
| effect of breast feeding on responses of systemic interferon and virus-specific lymphocyte transformation in infants with respiratory syncytial virus infection. | in order to elucidate the mechanisms of breast-feeding-induced resistance to respiratory syncytial virus (rsv) infection, groups of breast-fed and bottle-fed infants with this infection were tested at the onset of illness and then again 1 and 2 weeks later for the presence of interferon (ifn) as well as of virus-specific lymphocyte transformation (ltf) activity. alpha-ifn was detected in nine out of ten breast-fed infants, while it was found in only 11 out of 21 bottle-fed subjects. the rate of ... | 1987 | 3794673 |
| fatal pneumonia in an adult due to respiratory syncytial virus. | respiratory syncytial virus (rsv) infections in adults are generally mild, and no fatalities in uninstitutionalized adults have been reported. to our knowledge, we document herein the first case of fatal rsv pneumonia in a previously healthy elderly woman living at home, in whom complement fixation titers against rsv rose from less than 1:8 to reach 1:256 at death. cytoplasmic inclusions characteristic of rsv were detected at autopsy. | 1987 | 3827469 |
| [bronchiolitis. our clinical experience in the 4 years from 1981 to 1984]. | the clinical experience of 661 children with bronchiolitis is reported in four-years period to gain a better understanding of diagnosis and pathogenesis of bronchiolitis. upper airways infections, expiratory dyspnea, clear sound by chest percussion, vesicular rales and whistling by chest auscultation, air trapping on the chest radiography were considered as essential data of diagnosis. it was found in 595 patients: expiratory dyspnea, air trapping, vesicular r. and whistling in 85% and whistling ... | 1985 | 3837240 |
| respiratory syncytial virus and heart block--cause and effect? | a child aged 3 years who developed a respiratory syncytial virus infection is described. his admission was complicated by the development of an irregular pulse. he initially had a variable first or second degree heart block with transient electrocardiographic evidence suggestive of pericarditis and/or myocarditis and later developed complete heart block. he has remained asymptomatic for the past four years. a search for other causes of the heart block proved negative. it is tempting to suggest a ... | 1985 | 3859266 |
| seroprevalence of respiratory syncytial virus in free-ranging bighorn sheep. | sera from 187 (42%) of 447 free-ranging bighorn sheep (ovis canadensis) in 9 western states had antibody titers to respiratory syncytial virus (rsv). titers were from 1:5 to 1:640, as determined by the standard virus neutralization test. results of the survey indicated that rsv is widespread in free-ranging bighorn sheep populations and that rsv may be an important factor in the bighorn sheep pneumonia-complex. | 1985 | 3878355 |
| management of bronchiolitis. | the incidence, etiology, epidemiology, clinical presentation, treatment, prognosis, and prevention of bronchiolitis are discussed with a critical evaluation of the available studies on various therapeutic approaches. bronchiolitis is a lower respiratory-tract viral infection that affects 6-10% of all children below two years of age. respiratory syncytial virus (rsv) is the usual pathogen. the symptoms range from mild wheezing to severe respiratory distress. an infected child usually has a fever, ... | 1985 | 3891201 |
| properties of a respiratory syncytial virus isolated from a sheep with rhinitis. | a virus isolated from a yearling cross-bred ewe was identified as respiratory syncytial virus (rsv) by indirect immunofluorescence and by virus neutralization with bovine rsv antisera. the virus caused a mild conjunctivitis in 3-month-old lambs when inoculated alone. although clinical signs of pneumonia were not observed, there was gross and microscopic evidence of pulmonary inflammation in the lungs of lambs inoculated with either the sheep rsv isolate alone or in conjunction with pasteurella h ... | 1985 | 3893243 |
| quantification of respiratory syncytial virus polypeptides in nasal secretions by monoclonal antibodies. | an indirect enzyme-linked immunosorbent assay (elisa) which uses monoclonal antibody as solid-phase immunosorbent was developed to measure specific polypeptides of respiratory syncytial virus (rsv). the assay was used to examine 43 nasopharyngeal (np) aspirates from rsv-positive infants that had been examined previously for rsv by culture, direct immunofluorescence, and polyclonal antibody elisa. frozen np aspirates were serially diluted and examined for the 66k mol. wt. fusion glycoprotein (f), ... | 1985 | 3894575 |
| practical recommendations for the detection of pediatric respiratory syncytial virus infections. | in our private clinic-hospital setting, respiratory syncytial virus (rsv) was isolated from infants more frequently and sooner from nasal washes (84%; 4.2 days) than from throat swabs (45%; 5.5 days) or nasopharyngeal swabs (39%; 5.7 days). immunofluorescence of nasal wash cells identified 72% of the infants with virus isolations from nasal washes in less than one day. we therefore recommend the combination of isolation and immunofluorescence on nasal wash specimens for optimal detection of rsv- ... | 1985 | 3897268 |
| development of cell-mediated cytotoxic activity in the respiratory tract after experimental infection with respiratory syncytial virus. | the development of natural killer cell and other antibody-independent cellular cytotoxic response to rsv were studied in splenic and pulmonary mononuclear effector cells obtained from groups of 6-week-old cotton rats after subcutaneous (sc) or intranasal (in) immunization with live virulent or ultra-violet (uv) inactivated respiratory syncytial virus (rsv). no virus-induced cytotoxic activity was observed after sc immunization with live virus or in inoculation of inactivated non-infectious virus ... | 1985 | 3899431 |
| the production of monoclonal antibodies against respiratory syncytial virus and its clinical applications. | the availability of monoclonal antibodies against various viral components, either internal nucleoproteins or membrane proteins, has greatly advanced our understanding of the biochemistry of respiratory syncytial virus (rsv). the components responsible for serum neutralization have been identified by monoclonal antibodies, thus facilitating the development of synthetic rsv vaccine. a number of clinical tests have been devised using monoclonal antibodies for either qualitative or quantitative det ... | 1985 | 3899483 |
| immunoprophylaxis and immunotherapy of respiratory syncytial virus infection in the cotton rat. | human convalescent antiserum to respiratory syncytial virus (rsv) administered intraperitoneally to cotton rats prior to rsv challenge provided near-complete protection from pulmonary infection. antiserum given subsequent to viral challenge reduced pulmonary viral titers 100-fold or greater within 24 h. sandoglobulin, a preparation of purified human igg with high titer of anti-rsv neutralizing activity, produced the same effects as convalescent antiserum. sandoglobulin was absorbed rapidly and p ... | 1985 | 3907188 |
| calf pneumonia. | infectious calf pneumonia is a high-mortality pneumonia of housed dairy-type calves. viruses are important etiologic agents and infection with bovine respiratory syncytial virus (rsv) and parainfluenza type 3 virus (pi-3 virus) may result in extensive, and sometimes fatal, lung damage. respiratory viral infections are frequently followed by mycoplasmal and secondary bacterial invasion of the lower respiratory tract, which increases the extent and severity of lung damage. bad housing, particularl ... | 1985 | 3907774 |
| respiratory syncytial virus infection in immunosuppressed animals: implications in human infection. | neonatal cotton rats were treated with cyclophosphamide parenterally for three weeks before intranasal inoculation of live respiratory syncytial virus (rsv). immunosuppressive therapy resulted in severe depletion of lymphocytes from the peripheral circulation, the spleen, and the thymus. in contrast to normal rats, immunosuppressed animals developed severe pulmonary pathology with marked infiltration of foamy macrophages. persistent degeneration and regeneration of bronchial epithelial cells wer ... | 1985 | 3908616 |
| immunoprecipitation of insulin receptors from cultured human lymphocytes (im-9 cells) by antibodies to pp60src. | the family of tyrosine-specific protein kinases includes proteins encoded by retroviral oncogenes as well as receptors for insulin and several growth factors. antibodies to pp60src, the protein encoded by the src oncogene of rous sarcoma virus (rsv), can specifically immunoprecipitate affinity-labeled insulin receptors from cultured human lymphocytes (im-9 cells). this precipitation is specifically inhibited by the src gene product purified from rsv-transformed rat cells. these observations prov ... | 1985 | 3918346 |
| an n-terminal peptide from p60src can direct myristylation and plasma membrane localization when fused to heterologous proteins. | the src gene product, p60src, of rous sarcoma virus (rsv) is a tyrosine-specific protein kinase which is associated with the plasma membrane of infected cells. myristic acid is bound in an amide linkage to glycine 2 of p60src. of the n-terminal 30 kilodaltons of p60src, only amino acids 1-14 are required for myristylation, and myristylation of p60src may be required for its membrane association, and for cell transformation. to test the hypothesis that the first 14 amino acids of p60src contain a ... | 1985 | 3920530 |
| radiological findings in children with respiratory syncytial virus infection: relationship to clinical and bacteriological findings. | respiratory syncytial virus (rsv) is a frequent cause of bronchiolitis leading to acute admission to hospital in the winter months. a wide range of findings accompanies this disease and the appearances are seldom completely diagnostic. associated bacterial co-infections are common and we have shown an association with atelectasis among patients with pathogenic bacteria in the nasopharynx. | 1986 | 3951895 |
| complement activation by respiratory syncytial virus-infected cells. | respiratory syncytial virus (rsv), a major respiratory pathogen of children, has been speculated to cause disease by immunologic mechanisms. although circulating levels of complement (c) are normal during rsv infections, the role of c in respiratory tract secretion is unclear. since epithelial cells of the respiratory tract of children infected with rsv express viral surface antigens, the ability of rsv infected human cells to activate c was studied. rsv infected human cells (hela) were found to ... | 1986 | 3954599 |
| antibody response to respiratory syncytial virus structural proteins in children with acute respiratory syncytial virus infection. | the purified respiratory syncytial virus (rsv), randall strain contained 10 polypeptides (72,000 molecular weight [72k], 66k, 48k, 42k, 40k, 36k, 30k, 23k, 18k, and 15k), 8 of which proved to be virus specific, and polypeptides 48k and 23k were glycosylated. in addition, a high-molecular-weight (150k), virus-specific glycopolypeptide was immunoprecipitated from rsv-infected cell lysate. the antibody response in human sera serially collected from children with primary rsv infection was mainly dir ... | 1985 | 3973971 |
| monoclonal capture antibody elisa for respiratory syncytial virus: detection of individual viral antigens and determination of monoclonal antibody specificities. | an enzyme-linked immunosorbent assay (elisa) for respiratory syncytial virus (rsv) that employs solid-phase monoclonal antibodies was developed. rsv antigens bound by these monoclonal capture antibodies were detected by addition of a polyclonal bovine antiserum, followed anti-bovine enzyme conjugate and enzyme substrate. the sensitivity and specific of the assay were determined by titrations of the solid-phase antibodies and by antigen titrations with both unpurified rsv-infected cell culture ma ... | 1985 | 3981003 |
| [experimental infection of lambs with strains of the bovine respiratory syncytial virus]. | lambs were successfully infected with bovine strains of a respiratory syncytial virus. when the nomi reference strain and the local antonovo strain of the rsv were used i/v and via the trachea the animals reacted on the 3rd to 5th day with dizziness, dyspnea, conjunctivitis, nasal discharges, etc., more strongly manifested with the use of the antonovo strain. the temperature reaction started on the 3rd day and receded on the 7th day, with a second peak on the 8h day. serologically, rsv-antibodie ... | 1985 | 3992914 |
| lobar collapse with respiratory syncytial virus pneumonitis. | in a study of 30 children with uncomplicated respiratory syncytial virus (rsv) pneumonias, a high incidence of lobar collapse (8/30-26%) was noted. this involved the right upper lobe in seven patients and the left upper lobe in one patient. it is probably attributable to anatomical predispositions, sloughing of necrotic epithelium, and stimulation of mucus production. lobar collapse should be considered part of the spectrum of rsv pneumonitis. | 1985 | 4000751 |
| respiratory syncytial virus-specific ige responses following infection: evidence for a predominantly mucosal response. | in order to determine whether ige production occurs predominantly at mucosal or systemic sites, we studied the production of respiratory syncytial virus (rsv)-specific antibody in serum and nasopharyngeal secretions (nps) from 41 patients with rsv infection using an enzyme-linked immunosorbent assay. rsv-ige was found in higher titer in samples of nps than in simultaneously obtained serum specimens at all phases of illness. despite the excess dilution incurred in the collection process, rsv-ige ... | 1985 | 4000767 |
| severe respiratory syncytial virus infection: clinical features, nosocomial acquisition and outcome. | respiratory syncytial virus (rsv) frequently causes bronchiolitis in young infants. rsv infection was established by culture, serologic response, immunofluorescence of secretions or lung tissue or typical histology on pathologic examination of the lungs in 10 patients who were hospitalized at children's medical center, dallas. eight were infants 4 days to 3 months old, one was 9 months and one was 16 months old. seven patients were afebrile but had rapidly progressive diffuse infiltrates on ches ... | 1985 | 4000987 |
| quantitative aspects of passive immunity to respiratory syncytial virus infection in infant cotton rats. | the amount of passively acquired serum respiratory syncytial virus (rsv)-neutralizing antibodies required to protect the respiratory tract of cotton rats against infection was studied. infant cotton rats were inoculated intraperitoneally with various dilutions of a single pool of sera derived from cotton rats convalescent from rsv infection. after 24 h, these animals were inoculated with rsv intranasally. virus replication in the respiratory tract was suppressed in cotton rats which had a serum ... | 1985 | 4020957 |
| severe rsv bronchiolitis in an immunocompromised child. | a 9-year-old with immunodeficiency developed a severe, diffuse respiratory illness that necessitated mechanical ventilation. open lung biopsy revealed respiratory syncytial virus (rsv) as the sole pathogen. rsv detection should be included in the differential diagnosis of diffuse lung disease in an immunocompromised child. | 1985 | 4064461 |
| reverse transcriptase-containing particles induced in rous sarcoma virus-transformed rat cells by arginine deprivation. | incubation of rat cells transformed by rous sarcoma virus (rsv) in an arginine-deficient medium resulted in accumulation of particles in the culture medium. such particles did not appear when the transformed rat cells were incubated in a complete medium nor in the medium of primary rat cells which were incubated either in arginine-deficient or complete media. the particles which were released from the arginine-deprived transformed rat cells resemble c-type particles in their properties. these pa ... | 1972 | 4116137 |
| transcription of dna from the 70s rna of rous sarcoma virus. i. identification of a specific 4s rna which serves as primer. | the enzymatic transcription of dna from the 70s rna of rous sarcoma virus (rsv) is initiated on the 3' terminus of a molecule of 4s rna which is hydrogen bonded to the viral genome. we labeled this primer with radioactive deoxynucleotides, and demonstrated that its release from 70s rna by thermal denaturation was accompanied by a reduction in the template activity of the viral rna. two-dimensional electrophoresis in polyacrylamide gels separated the 4s rnas associated with the 70s rna of rsv int ... | 1974 | 4132919 |
| ultrastructure of the surfaces of cells infected with avian leukosis-sarcoma viruses. | when stained with ruthenium red (rr), chick embryo cells infected with various strains of rous sarcoma virus (rsv) and with avian leukosis viruses rav-1 and rav-3 showed an increase in the layer of acid mucopolysaccharides (amps) at their surfaces as compared with uninfected cells. this increase was most prominent in cells infected with the fujinami strain of rsv. the layer was resistant to digestion with neuraminidase or trypsin but was readily removed by exposure to hyaluronidase. the thicknes ... | 1968 | 4177656 |
| specific transplantation immunity in relation to rous sarcoma virus tumorigenesis in mice. | tests for transplantation immunity and for the occurrence of virus-neutralizing serum antibodies were performed on mice, inoculated when newborn with the schmidt-ruppin strain of rous sarcoma virus (rsv-sr). mice developing no palpable primary sarcomas showed a clear-cut resistance against the isografting of established specifically antigenic rous tumors. transplantation tests performed on primary tumor hosts after extirpation of the tumors revealed neither any clear-cut immunity nor tolerance t ... | 1966 | 4287060 |