Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| acute transmural myocardial infarction associated with active coxsackie virus b infection. | out of a total of 233 patients suffering from transmural myocardial infarction, 20 patients were found to have serologic evidence of a concurrent active coxsackie virus b infection. while the infection may have been coincidental, it is also possible that the virus may have played some part in the illness. conceivably, myocarditis could be mistaken for infarction or, by some mechanism as yet unknown, the virus might precipitate infarction in susceptible subjects. | 1975 | 1167729 |
| editorial: moves towards svd eradication. | 1975 | 1168004 | |
| electrophoretic analysis of capsid and non-capsid polypeptides of echovirus 12, and selective inhibtion of the formation of virus particles by actinomycin d. | electrophoretic analysis of purified echovirus virus particles yielded four polypeptides of mol. wt. 37000, 30000, 25000 and 7600. the 75s empty capsids of echovirus 12 lack the 25000 and 7600 mol. wt. polypeptides, and possess polypeptides of 41000 mol. wt. a total of 14 virus-induced polypeptide species was found in the cytoplasm of infected cells. actinomycin d reduced the synthesis of virus, virus rna, and virus polypeptides and also reduced the proportion of virus particles to empty capsid ... | 1975 | 1168242 |
| sequelae of central-nervous-system enterovirus infections. | the long-term effects of central-nervous-system enterovirus infections were examined in a controlled follow-up study of 19 children 2 1/2 to eight years of age who had been hospitalized with documented enterovirus infection 17 to 67 months before evaluation. assessment included medical history, physical and neurologic examination, psychologic testing, and speech and hearing evaluation. three children (16 per cent) had definite neurologic impairment, five (26 per cent) had possible impairment, an ... | 1975 | 1168853 |
| [virologic and serologic findings in an epidemic of abacterial meningitis of unknown origin (author's transl)]. | in the summer of 1972 there occurred an epidemic of abacterial meningitis in bayreuth and its surroundings. we received material of 52 ill children, aged 2 months to 14 years. a virus could be isolated in 10 (5 coxsackie a4, 2 echo 6, 1 coxsackie b 5 and twice enterovirus which would not be typed). only in another 13 children could we make unequivocal diagnoses (7 coxsackie a4, 2 coxsackie b5, one each coxsackie a 2, echo 3, mumps, rrs-virus). with the other 29 patients a safe laboratory diagnos ... | 1975 | 1172156 |
| prevalence of hepatitis b antibody in prostitutes. | the possibility that hepatitis b is transmitted venereally was examined by determining the prevalence of hepatitis b antibody (anti-hb(s)) in populations with different levels of promiscuity. of 258 prostitutes, 31% were anti-hb(s) positive, compared to 10% of 258 female age-matched blood donors, 10% of 48 nuns living in a convent and 15% of 94 nuns working as teachers. prevalence of anti-hb(s) increased with increasing age. in the age group 40-49 years up to 72% of prostitutes were anti-hb(s) p ... | 1975 | 1174290 |
| propagation and isolation of group a coxsackieviruses in rd cells. | the rd cell line, derived from a human rhabdomyosarcoma, supported replication of a number of group a coxsackieviruses, including types a5 and a6 which heretofore have been propagable only in suckling mice. a number of the group a coxsackievirus types which replicated in rd cells had higher titers in this cell line than in other cell culture systems. in tests on a limited number of clinical specimens, rd cells were slightly less sensitive than suckling mice for isolation of group a coxsackieviru ... | 1975 | 1176625 |
| hand, foot and mouth disease associated with coxsackievirus group b. | this study from frankfurt, although similar to other reports with respect to clinical findings, presents further evidence of a varied etiology for hand, foot and mouth disease (hfmd). of the 6 patients with hfmd, 5 ahd virologic evidence of coxsackievirus b5 infection, and 1 had definite evidence of coxsackievirus b2 infection. our data suggest an etiologic association of coxsackievirus group b with hfmd. | 1975 | 1179172 |
| a local outbreak of paralytic rabies in surinam children. | a rapidly fatal encephalomyelitis, which was in most cases characterized by ascending paralysis, developed in seven children of the age of 3 to 10 years in a bushnegro village in the interior of surinam. rabies virus was recovered from the central nervous system of three autopsied children. although the source of infection has not been detected, there is an indication that, at least in some cases, the disease has been transmitted by rat-bite rather than by vampire bats. during the same period a ... | 1975 | 1179478 |
| epidemic pleurodynia (bornholm disease) outbreak in singapore. a clinical and virological study. | in 1974, an outbreak of bornholm disease occurred in singapore. the period 1st may to 31st july was delineated for study. from the clinical presentation 53 patients were placed into two categories "typical bornholm disease" and "atypical bornholm disease". the clinical features of only those in the "typical bornholm disease" group including those with positive coxsackie b3 virus isolation were described. the virological studies, both faecal isolation for virus and serology were correlated with c ... | 1975 | 1179480 |
| complete heart block in viral myocarditis. | 1975 | 1185359 | |
| multiplication of coxsackie b1 virus in synchronized hela cells. | a higher yield of coxsackie b(1) virus was obtained when hela cells were infected late during s phase as compared to the amount produced by random cultures. | 1975 | 1185854 |
| generalized myopathy and cerebral malformations possibly related to an enteroviral infection. | a study was made on a case of generalized muscular hypotonus manifested at birth. serological findings and epidemiological data suggested an association to a recently described enterovirus infection (enterovirus candidate 71) known to cause neurological disease in man. autopsy revealed cerebral malformations and generalized myopathy compatible with a viral etiology of the disease. | 1975 | 1189912 |
| viral antibody studies in pancreatic disease. | viral studies were performed on sera from 54 patients with recent acute pancreatitis, 10 with recurrent acute pancreatitis, seven with chronic pancreatitis, and 10 with pancreatic carcinoma, and on sera from 81 age- and sex-matched controls. in 29 of the acute pancreatitis patients from whom paired sera were obtained no convincing evidence of recent viral infection was found. a higher incidence of raised antibody titres against coxsackie b3 and b4 was observed in the group of acute pancreatitis ... | 1975 | 1193416 |
| virologic and serologic studies on an outbreak of echovirus type 11 infection in a hospital maternity unit. | an outbreak of echovirus type 11 (e-11) infection occurred among newborn babies in a hospital maternity unit in the summer of 1971. the results of studies are as follows: 1) forty-one of 188 infants developed febrile illness with stomatitis during one and a half months from july to september. e-11 was isolated from stool specimens of 14 infants and two throat swabs. antibody response to the virus was shown in all the 19 cases examined. some of their mothers were suffering from subclinical infect ... | 1975 | 1195517 |
| oral polio vaccination of children in the tropics. iii. intercurrent enterovirus infections, vaccine virus take and antibody response. | the effect of intercurrent enterovirus infections on host responses to oral polio vaccine (opv) was studied in groups of infants and children who were without antibodies to one, two or three serotypes of poliovirus. the prevalence of enterovirus infections as detected in fecal specimens collected at weekly intervals and inoculated in primary monkey kidney cell culture, hep 2 cells and newborn mice ranged between 60 and 70 per cent. the presence of such infections at the time of, 1 week prior to, ... | 1975 | 1200026 |
| [epidemic of lymphocytic cerebrospinal meningitis in the fall of 1974 at grudziadz]. | in 1974 an epidemic of lymphocytic cerebrospinal meningitis occurred in the province of bydgoszcz. thirty-nine cases of this disease were hospitalized at the neurological department of the hospital in grudziadz. virological investigations demonstrated presence of coxsackie a9 virus. the manifestations of the disease, diagnostic methods and treatment are described. | 1975 | 1202400 |
| serosurvey for "acute hemorrhagic conjunctivitis" virus (enterovirus 70) antibodies in the southeastern united states, with review of the literature and some epidemiologic implications. | acute hemorrhagic conjunctivitis (ahc) has been epidemic throughout much of the eastern hemisphere since its emergence in central west africa in 1969. the disease had a distinctive clinical picture and an unusual geographic epidemiology. between 1969 and 1975 ahc has occurred almost exclusively in crowded coastal areas of tropical countries during hot, rainy seasons. only a few documented outbreaks have occurred in inland cities and in subtropical or temperate climate zones. of 1014 residents of ... | 1975 | 1202955 |
| hemopericardium associated with coxsackie b 6 viral infection. | 1975 | 1206257 | |
| a comparative study of meningoencephalitis epidemics caused by echovirus type 7 and coxsackievirus type b5. clinical and virological observations during two epidemics in northern sweden. | two epidemics of meningoencephalitis caused by echovirus type 7 and coxsackievirus type b 5 in the summer and autumn of 1973 in umeå in northern sweden were compared. most patients with echovirus 7 meningoencephalitis were neck stiff and 50% had a polymorphonuclear pleocytosis in the cerebrospinal fluid (csf). the illness was usually mild. it appears to be the first time that an epidemic caused by this virus is described from scandinavia. on the other hand most patients with coxsackievirus b 5 m ... | 1975 | 1209167 |
| echovirus type 4 infections in scotland, 1971-72. | an outbreak of infection due to echovirus 4 in scotland involving 194 cases during 1971-72 is described. clinical and epidemiological information was obtained and analysed for 181 patients, of whom 149 (82%) developed aseptic meningitis. the majority of cases were older children (39%) and young adults (40%), although the highest attack rate was in infants (10.5 per 100,000 population). an unusual feature of the outbreak was its biphasic nature with cases occurring largely in the same geographic ... | 1975 | 1209168 |
| studies on some biological properties of heteroploidal cell -- pzh-1 line derived from human embryo lung. | the morphological and karyological properties as well as susceptibility to virus strain of tissue culture derived from human embryo lung that underwent a transformation becoming a continuous line defined as pzh-1 were observed. the observations cover 120 passages divided into four phases: one before and three after transformation. each of these phases was characterised by different biological strain properties. loss of pzh-1 susceptibility to echo virus was the first change of biological propert ... | 1975 | 1211221 |
| spherical aggregates of coxsackie b4 virus particles in mouse pancreas. | a high titer culture of coxsackie b4 virus was used to induce pancreatitis in newborn mice. in animals sacrificed 1 or 2 days after intraperitoneal inoculation, we observed cytonecrosis consistent with picornaviral infection as well as necrosis indicative of pancreatitis. in addition, we observed aggregates of particles which seem to be coxsackie b4 virus particles, some arranged in the typical picornaviral crystalloid lattice formation and others arranged into spherical masses approximately 102 ... | 1975 | 1212165 |
| editorial: the recent epidemic of conjunctivitis. | 1975 | 1214065 | |
| the coronary arteries in active viral cardiomyopathies. | 1975 | 1215244 | |
| [an epidemic of viral meningitis in the city of grudziadz in 1974]. | 1975 | 1215548 | |
| [epizootiology of swine vesicular disease]. | 1975 | 1216655 | |
| [peculiarities of inorganic phosphate metabolism in the coxsackie b-1 virus-kb cell system]. | 1975 | 1219327 | |
| [therapeutic trial with a single oral dose of pyrantel pamoate in infections by enterovirus vermiculosis and ascaris lumbricoides]. | 1975 | 1219940 | |
| [development of optimal conditions for isolation of rhinoviruses]. | when 366 dispensary patients with respiratory diseases were examined in moscow during the period december 1969--may 1971, 49 strains of rhinoviruses were isolated out of which 21 strains were identified as type 48. using eagle's medium with a single set of amino acids and vitamins for isolation rhinoviruses were isolated in 4.7% of cases, empdoying eagle's medium with a double set of amino acids and vitamins in 16% of cases. virus isolation experiments were carried out in hela-bristol cell cultu ... | 1975 | 1220246 |
| variations in coxsackie virus pathogenicity in the course of routine isolations in suckling mice and cell cultures. | from 1961 to 1973 416 coxsackie viruses were isolated, most of the isolations (303) being done in the last 5 years. most of the isolates belong to a group a, with a predominance of types a6 and a8. many variations were observed in strains belonging to the same type, as concerns their isolation in human embryo cell cultures and suckling mice; many coxsackie a5, a6 and a8 strains showed a low pathogenicity for these animals. | 1975 | 1224536 |
| [letter: combination of repeated vaccinations with oral vaccination]. | 1975 | 1225067 | |
| [effect of immunodepression on virus multiplication and interferon and antibody formation in animals]. | administration of high doses of imuran had no significant effect on the multiplication of influenza a/pr8 and coxsackie a6 viruses. however, the serum levels of interferon and antibody were completely suppressed. peripheral leukocytes of mice given imuran produced no interferon in vitro. these data suggest that the impairment of formation of the antiviral immunity factors resulting from the administration of high doses of immunosuppressants, such as used during the graft rejection crises, could ... | 1975 | 1225400 |
| [the value of the coxsackie b virus complement fixation test (author's transl)]. | enteroviruses were isolated from 27 patients during 1973 and 1974, 21 of these were coxsackie b, 4 were echo and 2 were polio viruses, 5 further viruses could not be identified. sera from 14 of the coxsackie patients were investigated twice by means of the complement fixation test (cft). 11 of them showed a significant rise in antibody titre against one of the 6 different coxsackie b viruses. no strong correlation was established between the result of the cft and the isolated type of virus. neve ... | 1975 | 1226771 |
| sporadic cases associated with non polio enteroviruses studied in lombardy between 1965 and 1972. | the results of virological investigations on 492 sporadic cases with a clinical diagnosis compatible with an enterovirus etiology studied in lombardy between 1965 and 1972 are reported. a recent infection with non polio enteroviruses was established in 54 patients, mostly children under 4 years of age. coxsackie viruses group a were prevalent being associated with 38 cases; echoviruses were involved in 13 cases, coxsackie b viruses only in 3 instances. the frequency of isolation was highest from ... | 1975 | 1230061 |
| summarized report of research works by the members of the virology subsection, the microbiology section. | 1975 | 1234599 | |
| counterelectrophoresis on human serum in coxsackie virus infections. | 1975 | 1236634 | |
| [isolation of coxsackie a type 16 and other types of coxsackie a group viruses from hand, foot and mouth disease patients in hokkaido in summer, 1970 (author's transl)]. | 1975 | 1241630 | |
| familial hepatic venoocclusive disease with probable immune deficiency. | five infants from three families died between the ages of 2 and 7 months with venocclusive disease of the liver. no dietary, toxic, or other extrinsic cause was uncovered. in one family the first infant was breast-fed; the second one received no breast milk. in two of the families the parents were cousins. all infants had some evidence of immune deficiency, including hypogammaglobulinemia in at least three, multiple infections especially pnumocystis carnii and enteroviruses, and lymphoid tissues ... | 1976 | 1249685 |
| interferon and neutralizing antibody in sera of exercised mice with coxsackievirus b-3 myocarditis. | weanling icr albino swiss mice were inoculated ip with 1.9 x 10(4) pfu of coxsackievirus b-3 (nancy) and subsequently forced to swim vigorously daily in a preheated pool (33 degrees). viremias and virus in hearts of exercised mice were respectively 75 x 1000 x greater than in infected, but not exercised mice. at 24 hr after inoculation, pooled serum from mice that had been swum had no circulating interferon, while infected but not swum mice had interferon activity at a dilution of 1:10. at 72 hr ... | 1976 | 1250870 |
| oyster-associated hepatitis: lessons from the louisiana experience. | an outbreak of oyster-associated hepatitis occurring in louisiana during the months of october and november 1973 has been shown to be related directly to two similar outbreaks occurring at the same time in houston, texas and calhoun, georgia. oysters incriminated in all three outbreaks were traced to approved oyster growing areas along the east louisiana coast. although no entirely satisfactory explanation could be found for the mode of contamination of these oysters, the epidemiologic and envio ... | 1976 | 1251833 |
| autoimmunity and viral infection in diabetes mellitus. | forty-three newly diagnosed insulin-dependent diabetics and thirty control subjects have been examined for cellular hypersensitivity to different pancreatic preparations, rat liver mitochondria and coxsackie b4 virus. evidence for cellular hypersensitivity to the pancreatic preparations was shown in the diabetic group, in whom the hypersensitivity to the pancreatic preparations was found to correlate with hypersensitivity to rat liver mitochondria. hypersensitivity to coxsackie b4 virus was not ... | 1976 | 1261089 |
| application of systolic time intervals to acute cardiomyopathy with echovirous 2. | the occurrence of echovirus type 2 myocarditis in an adult is reported. myocarditis was substantiated by abnormal electrocardiograms. more important, a prolonged course and severe functional impairment was quantitatively assessed by serial systolic time intervals, even after the ecgs became normal. the patient also had meningitis, hemiparesis, and convulsions. viral isolation was from the spinal fluid. | 1976 | 1261332 |
| maturation of intestinal defenses against peroral infection with group b coxsackievirus in mice. | the intestinal tract of adult mice provides effective protection against peroral infection with group b coxsackievirus. this protective function consists of at least two separate components. one is a barrier effect that prevents virus from passing through the mucosal side of the gut into the circulation. it becomes clearly evident at 18 days of life and is present thereafter. the other is a clearance mechanism that acts to eliminate virus from the enteric tract after infection has occurred. this ... | 1976 | 1270146 |
| successful treatment of enterovirus-infected mice by 2-(alpha-hydroxybenzyl)-benzimidazole and guanidine. | echo virus 9- or coxsackie a 9-infected newborn mice are protected from paralysis and death by combined treatment with nontoxic concentrations of hbb plus guanidine. hbb alone also protects coxsackie a 9, but not echo virus 9-infected animals, whereas guanidine alone is ineffective in either case. protection is due to inhibition of virus multiplication via the antiviral activity of these selective inhibitors. treatment must be begun at the latest 48 h after virus inoculation. 3 days of treatment ... | 1976 | 1271013 |
| letter: antibody titers to coxsackieviruses in amyotrophic lateral sclerosis. | 1976 | 1272306 | |
| [basis of the differentiation of the symptoms of viral enteritis of geese]. | 1976 | 1274163 | |
| detection of norwalk virus in stool specimens by reverse transcriptase-polymerase chain reaction and nonradioactive oligoprobes. | a reverse transcriptase (rt)-polymerase chain reaction (pcr)-oligoprobe (op), or rt-pcr-op, method was developed for the detection of the norwalk virus, which causes acute, epidemic gastroenteritis, in stool specimens. the norwalk virus genome regions encoding the following two proteins were amplified by rt-pcr: the rna polymerase (260-bp product) and a putative immunogenic protein (224-bp product). the resulting dna fragments (amplicons) were hybridized to a digoxigenin-labeled internal op spec ... | 1992 | 1280649 |
| [the inhibitory effects of catechin derivatives on the activities of human immunodeficiency virus reverse transcriptase and dna polymerases]. | catechin derivatives including (-)-epicatechin gallate (ecg), (-)-epigallocatechin gallate (egcg), (-)-epigallocatechin (egc) and green tea extract (gte) were found to inhibit the activities of cloned human immunodeficiency virus type 1 reverse transcriptase (hiv-1 rt), duck hepatitis b virus replication complexes reverse transcriptase (dhbv rcs rt), herpes simplex virus 1 dna polymerase (hsv-1 dnap) and cow thymus dna polymerase alpha (ct dnap alpha). egcg and ecg were shown to be very potent i ... | 1992 | 1284389 |
| [the standardization of a "dot-blot" method for the molecular identification of enterovirus in environmental samples using cdna probes]. | 1992 | 1284585 | |
| general principles of laboratory methods for virus detection in environmental samples. | 1992 | 1284905 | |
| food as enteric viruses vehicle. | 1992 | 1284906 | |
| echovirus surveillance. | 1992 | 1285234 | |
| complement fixing antibodies against selected viruses in diabetic patients and non-diabetic control subjects in ibadan, nigeria. | sera of forty insulin-treated nigerian diabetics attending the outpatients clinic of the university college hospital (u.c.h.), ibadan, and an equal number of non-diabetic control subjects matched for age, sex and social status were tested for presence of complement fixing (cf) antibodies against five viruses: coxsackie a and b1, mumps rubella and enterovirus group antigen. antibody levels to coxsackie b1, mumps, rubella and enterovirus did not show any significant difference between the two grou ... | 1992 | 1288637 |
| genetic factors in demyelinating diseases: genes that control demyelination due to experimental allergic encephalomyelitis (eae) and theiler's murine encephalitis virus. | 1992 | 1290751 | |
| vacuolar neuronal degeneration in the ventral horns of scid mice in naturally occurring theiler's encephalomyelitis. | during a spontaneous outbreak of theiler's encephalomyelitis severe combined immunodeficient mice developed high morbidity and high mortality. histological lesions were localized in the ventral horns of the spinal cord and brain stem. the salient features were the severe vacuolar degeneration of neurones and glial cells and the absence of inflammatory cellular infiltrates. the clinical and pathological features of this outbreak indicate that the scid mouse would be a much improved model for stud ... | 1992 | 1291587 |
| protective effect of a monoclonal antibody specific for an echovirus cellular receptor in human fibroblast and simian kidney cell lines. | we previously reported that infection of kb cells by echoviruses (ev) was inhibited by a kb-derived ev receptor murine monoclonal antibody (mab 143). this antibody enabled the identification of a cellular receptor common to all echoviruses (with the exception of ev-22 and -23) and coxsackievirus (cv) a9, but different from the receptor of other picornaviruses. we now present results of cell protection assays conducted with human and simian cell lines different from the kb cell line used for prod ... | 1992 | 1297174 |
| [the humoral immunity indices of patients with malignant skin melanoma using the viral immunomodulator rigvir]. | the effect of a viral immunomodulator rigvir on humoral immunity was studied in patients with skin malignant melanoma. peripheral blood levels of b-lymphocytes, iga, g and m and circulating immune complexes were assayed and immunoglobulin/b-cell ratio (ig/b) calculated. preoperative treatment with rigvir brought the indexes of humoral immunity to normal. response of melanoma patients to rigvir treatment was different from that seen in healthy subjects and was determined by the course of disease. | 1992 | 1300751 |
| [the cellular immunity indices of patients with malignant melanoma using the viral immunomodulator rigvir]. | the effect of rigvir, an immunomodulator of the viral origin, on cell-mediated immunity was studied in patients with skin malignant melanoma. rosette formation and monoclonal antibody techniques were used to measure blood immunocompetent cell levels in patients with the above pathology, cases of benign skin tumors and healthy subjects. rigvir was shown to influence natural resistance by raising blood monocyte and large granule-containing lymphocyte levels. it potentiated recruitment of pre-t-lym ... | 1992 | 1300752 |
| [the reaction of the t-immunity system in patients with malignant skin melanoma and stomach cancer to active nonspecific immunotherapy]. | changes in e-receptor-bearing t-lymphocyte level (total and that of active t-lymphocytes) were studied in peripheral blood and resected material obtained from skin malignant melanoma and gastric cancer patients treated with rigvir, an original immunomodulator of the viral origin. injection of rigvir into peripheral blood was followed by an increase in active t-lymphocyte level and stimulated their migration into tumor. the latter was determined by stage and rate of tumor advancement. | 1992 | 1300766 |
| acute hemorrhagic conjunctivitis. | acute hemorrhagic conjunctivitis, an infection caused by enterovirus 70 and a variant of coxsackievirus a24, is characterized by the rapid onset of severely painful conjunctivitis and subconjunctival hemorrhage. the condition is usually benign and resolves in five to seven days; however, a polio-like paralysis (radiculomyelitis) develops in approximately one in 10,000 patients infected with enterovirus 70. no treatment is available. information about acute hemorrhagic conjunctivitis should be pr ... | 1992 | 1309404 |
| ongoing enterovirus-induced myocarditis is associated with persistent heart muscle infection: quantitative analysis of virus replication, tissue damage, and inflammation. | coxsackievirus b3-induced myocarditis in different immunocompetent mouse strains was used as a model to investigate interrelationships between virus replication and development of chronic enteroviral heart disease. using in situ hybridization to detect enteroviral rna, we show that heart muscle infection is not only detected in acute myocarditis but is also detected during the chronic phase of the disease. coxsackievirus b3 could evade immunological surveillance in a host-dependent fashion, thus ... | 1992 | 1309611 |
| role of t cell receptor v beta genes in theiler's virus-induced demyelination of mice. | intracerebral infection of certain strains of mice with theiler's virus results in chronic immune-mediated demyelination in spinal cord. we used mouse mutants with deletion of the v beta class of tcr genes to examine the role of tcr genes in this demyelinating disease which is similar to multiple sclerosis. quantitative analysis of spinal cord lesions demonstrated a markedly increased number and extent of demyelinated lesions in persistently infected riii s/j mice which have a massive deletion o ... | 1992 | 1309845 |
| viral myocarditis--a tale of two diseases. | 1992 | 1309925 | |
| enteroviral infection of mice with severe combined immunodeficiency. evidence for direct viral pathogenesis of myocardial injury. | inbred mice with genetically determined severe combined immunodeficiency (scid) lack mature t and b lymphocyte functions. to distinguish direct viral effects in the pathogenesis of myocarditis from those mediated by antigen-specific and histocompatibility-complex-restricted host immunity, we inoculated coxsackievirus b3 into homozygous young adult scid mice. we found that infected scid mice invariably developed extensive myocarditis between 7 and 14 days postinoculation with high subsequent mort ... | 1992 | 1309927 |
| coxsackievirus b3 from an infectious cdna copy of the genome is cardiovirulent in mice. | a coxsackievirus b3 (cvb3) cdna clone, upon transfection of hela cells, produces cvb3 capable of induction of cardiac inflammation in c3h/he mice by day 8 post inoculation (p.i.). liver and serum are cleared of cvb3 by day 8 p.i., but cvb3 persists in the heart through day 14. the nucleotide sequence and the predicted amino acid sequence of this clone have sites of divergence from 2 other completely sequenced cvb3 genomes although overall identity of the three cvb3 genomes is 99%. | 1992 | 1310000 |
| characterization of the roles of conserved cysteine and histidine residues in poliovirus 2a protease. | the primary processing of the poliovirus polyprotein is catalyzed by 2a protease (2apro) which cleaves at the 1d/2a junction in a very rapid cotranslational reaction. in addition, 2apro also indirectly induces cleavage of the p220 component of eif-4f, which results in selective inhibition of host protein synthesis. earlier studies have indicated that 2apro is related to 3c protease (3cpro) and is structurally similar to trypsin-like serine proteases with the substitution of cys109 as the nucleop ... | 1992 | 1310193 |
| ultrastructural alterations of the conduction system in mice exhibiting sinus arrest or heart block during coxsackievirus b3 acute myocarditis. | by light and electron microscopy we studied the sinus nodes and atrioventricular (av) conducting tissue of six c3h/he mice having coxsackievirus b3 acute myocarditis. sinus arrest was documented in all six mice, and second- or third-degree av block was documented in three of the six mice. although myocarditic changes in the conduction system, especially in the sinus node, were less than those in atrial and ventricular working myocardium, there were distinct abnormalities within both the sinus no ... | 1992 | 1310565 |
| association of heat shock protein 70 with enterovirus capsid precursor p1 in infected human cells. | members of the human heat shock (hsp) family of related proteins are involved in the intracellular folding, transport, and assembly of proteins and protein complexes. we have observed that human heat shock protein 70 (hsp70) is associated with the capsid precursor p1 of poliovirus and coxsackievirus b1 in infected hela cells. antiserum generated against hsp70 coimmunoprecipitated the poliovirus protein p1, an intermediate in capsid assembly. similarly, alpha-virion serum coimmunoprecipitated hsp ... | 1992 | 1310763 |
| cytotoxic and viral neutralizing antibodies crossreact with streptococcal m protein, enteroviruses, and human cardiac myosin. | the development of autoimmunity in certain instances is related to infectious agents. in this report, cytotoxic monoclonal antibodies (mabs) that recognize epitopes on both enteroviruses and the bacterium streptococcus pyogenes are described. murine anti-streptococcal mabs that were crossreactive with streptococcal m protein, human cardiac myosin, and other alpha-helical coiled-coil molecules were found to neutralize coxsackieviruses b3 and b4 or poliovirus type 1. the viral-neutralizing anti-st ... | 1992 | 1311095 |
| search for coxsackievirus b3 rna in idiopathic dilated cardiomyopathy using gene amplification by polymerase chain reaction. | a polymerase chain reaction (pcr) amplification assay was developed to detect coxsackievirus b3 ribonucleic acid (rna) in blood and myocardial tissue of explanted hearts from 40 patients who underwent cardiac transplantation and in 1 normal heart. twenty-one patients were affected by idiopathic dilated cardiomyopathy of different duration and 19 by coronary artery disease. coxsackievirus b3 in vitro infected vero cells and cells infected by related human enteroviruses (coxsackievirus b2, b4, and ... | 1992 | 1311139 |
| crystallization and preliminary x-ray diffraction studies of coxsackievirus b1. | preparations of coxsackievirus b1 (cvb1) derived from an infectious cdna clone have been crystallized in multiple crystal forms. using high intensity synchrotron radiation, an orthorhombic form of the crystals was shown to diffract x-rays to at least 2.9 a resolution. the unit cell has a primitive lattice with dimensions a = 323 a, b = 450 a, and c = 522 a. a crystallographic asymmetric unit of these cvb1 crystals probably contains an entire virus particle, implying the presence of 60-fold non-c ... | 1992 | 1311388 |
| focal ventricular thinning caused by indomethacin in the late phase of coxsackievirus b4 murine myocarditis. | indomethacin has been shown to increase virus titers and to worsen cardiac injury in the acute phase of coxsackievirus b4 murine myocarditis. the authors evaluated the effects of indomethacin on the histopathologic changes in a later phase of this disease after virus clearance. two-day old cd1 mice were infected with coxsackievirus b4. ten days later, surviving animals were randomized to receive indomethacin or saline intraperitoneally for 10 days. they were then euthanatized, and their hearts w ... | 1992 | 1311498 |
| transplacental infection of porcine fetuses following experimental challenge inoculation with encephalomyocarditis virus. | ten multiparous sows were inoculated between 46 and 50 days of gestation with a fetal swine isolate of encephalomyocarditis virus (emcv) to investigate the ability of the virus to cause transplacental infection and fetal death. four sows (group 1) were inoculated im with emcv mn-25 that had been passaged 4 times on baby hamster kidney-21 line cell monolayers. two sows were euthanatized at postinoculation (pi) day 23, and the other 2 sows at pi day 44. an additional 6 sows (group 2) were inoculat ... | 1992 | 1311538 |
| mumps virus infects beta cells in human fetal islet cell cultures upregulating the expression of hla class i molecules. | the ability of mumps virus to infect pancreatic beta cells and cause alterations in their hla expression was evaluated in cultured human fetal islet cell clusters. mumps virus could be isolated during the whole culture period (6-8 days) and 60% of cells, including beta cells, contained viral nucleocapsid protein at the end of the culturing. a minor decrease in insulin secretion was observed in some of the infected cultures. the infection was invariably associated with an increase in the expressi ... | 1992 | 1311693 |
| [adenovirus infections in an emergency department]. | we have reviewed 108 emergency ward reports of patients with adenoviral positive cultures in "doce de octubre" hospital in madrid. our aim was to know the most frequent symptoms of the infection so that identification and treatment could be managed more efficiently in our department. cellular cultures were negative for other viruses such as cmv, enterovirus and herpesvirus. no bacteria were found in the throat cultures. of the children who were evaluated, 78% were younger than 3 years old. the m ... | 1992 | 1311905 |
| detection of coxsackie b virus myocarditis on tc-99m pyp myocardial imaging. | 1992 | 1312000 | |
| an experimental model of acute and subacute viral myocarditis in the pig. | twenty-six young pigs were infected with encephalomyocarditis virus, observed clinically, studied at intervals by noninvasive and invasive methods to assess cardiac function and eventually examined pathologically. all infected animals appeared ill, usually manifesting diminished appetite, lethargy and fever. spontaneous mortality occurred either 1 to 4 or 20 to 21 days after infection. electrocardiographic abnormalities, seen in the majority of animals, comprised st-t wave changes, conduction di ... | 1992 | 1312100 |
| coxsackievirus b3-induced production of tumor necrosis factor-alpha, il-1 beta, and il-6 in human monocytes. | infections by coxsackievirus b3 (cvb3) have previously been shown to cause acute and chronic myocarditis characterized by a heavy mononuclear leukocyte infiltration and myocyte necrosis. because clinical and experimental evidence suggested that cardiac damage may result from immunologic rather than viral mechanisms, we examined in this study the in vitro interaction of cvb3 with human monocytes. cvb3 was capable of infecting freshly harvested monocytes as revealed by immunofluorescence and relea ... | 1992 | 1312105 |
| the nucleotide sequences of wild-type coxsackievirus a9 strains imply that an rgd motif in vp1 is functionally significant. | we have shown previously that, compared to other enteroviruses, the coxsackievirus a9 (cav-9) prototype strain, griggs, contains a c-terminal extension to the capsid protein vp1 and that within this extension there is an rgd (arginine-glycine-aspartic acid) motif. to determine whether these features are found in other cav-9 strains and therefore analyse whether they are likely to be functionally important, we have determined the nucleotide sequence of the appropriate region from five strains, is ... | 1992 | 1312121 |
| phylogenetic analysis of a coxsackievirus a24 variant: the most recent worldwide pandemic was caused by progenies of a virus prevalent around 1981. | nucleotide substitutions in the viral-encoded proteinase 3c (3cpro) region (549 nucleotides) of the rna genome of a coxsackievirus a24 variant (ca24v), one of the agents causing acute hemorrhagic conjunctivitis (ahc), were studied using 32 isolates collected from the eastern hemisphere in 1970-1989. based on regression analysis of nucleotide differences among isolates, the nucleotide substitution rate of ca24v 3cpro was estimated to be 3.7 x 10(-3)/nucleotide/year. a phylogenetic tree constructe ... | 1992 | 1312276 |
| a 2-year survey of the prevalence of enteric viral infections in children compared with contamination in locally-harvested oysters. | we studied, for two years, the prevalence of indigenous human enteric viruses in wild oysters gathered each month from the bottom of mikawa bay, aichi prefecture, japan. viruses were detected periodically in 9 out of 54 oyster pools prepared by the acid or polyethylene glycol precipitation method although all these 9 pools met current national bacteriological safety standards. since most of the serotypes of the enteric viruses detected in the oysters were identical with those of viruses isolated ... | 1992 | 1312478 |
| the possible role of coxsackie a and echo viruses in the pathogenesis of type i diabetes mellitus studied by igm analysis. | igm antibodies to coxsackie b virus (cbv) have recently been observed in the serum of a relatively high proportion of children with newly diagnosed insulin-dependent diabetes mellitus (iddm). in the present study, 108 iddm patients below the age of 15 years, diagnosed during the period 1976 to 1985, were investigated at the onset of their disease by mu-antibody-capture radioimmunoassay (ria) of igm against seven different enterovirus antigens, namely virions of cbv serotypes 1-5 (cbv 1-5) and pr ... | 1992 | 1312561 |
| viral genomic detection in the hearts of c3h/he mice with experimental coxsackievirus b3 myocarditis by gene amplification using the polymerase chain reaction. | on the basis of the detection of the enteroviral rna in the hearts of patients with healed myocarditis and dilated cardiomyopathy, we investigated cardiac viral persistence in experimental myocarditis. weanling c3h/he mice were given myocarditis by inoculation with coxsackievirus b3 (nancy strain), and their hearts were examined by genomic studies and viral isolation up to the 180th day after inoculation. the virus was isolated from the heart until the 9th day. by slot-blot hybridization, viral ... | 1992 | 1312649 |
| [diagnosis of bacterial infections on vascular prosthesis. histological and microbiological analysis]. | a retrospective study of 212 vascular graft failures, using different criteria of infection evidenced a relatively high incidence of infectious complications. vascular graft infection was assessed on one of the following criteria: clinical infection, positive bacteriological and/or virological examination of the graft, presence of characterized micro-organisms and of microstructures 0.1 to 0.5 micron in size at the blood/prosthesis surface at scanning electron microscopy, and presence of foci of ... | 1992 | 1313557 |
| [coxsackie b4 and cytomegalovirus in patients with insulin-dependent diabetes]. | the purpose of this work is to analyze the potential relationship between infection by coxsackie and cytomegalovirus and the appearance of insulin-dependent diabetes (idd). we have assessed complement fixing (cf) antibodies against both viruses in 22 samples of serum from other individuals, among which 11 were patients with a recent diagnosis of idd--less than 3 months of evolution--(gp) and the remainder 11, supposed healthy individuals with similar age, sex and socioeconomic level (gc). in add ... | 1992 | 1313709 |
| a mouse model of coxsackievirus myocarditis. | to develop a mouse model of coxsackievirus b3 (cvb3) myocarditis. | 1992 | 1313735 |
| correlation between coxsackie b1 virus replication and enhanced invasiveness of shigella flexneri. | coxsackie b1 virus infection enhances the susceptibility of cultured hep-2 cells to shigella flexneri invasiveness. this can be reproduced partially with uv-inactivated virus, particularly the effect observed shortly after viral inoculation. the following phases of viral multiplication were correlated with the enhancing effect: uncoating of viral particles, synthesis of viral rna and proteins, and assembly of newly produced virus particles. uncoating of virus particles was completed within 60 mi ... | 1992 | 1314061 |
| coxsackie b virus igm in children at onset of type 1 (insulin-dependent) diabetes mellitus: evidence for igm induction by a recent or current infection. | thirty-five children with newly-diagnosed type 1 (insulin-dependent) diabetes mellitus and their 47 siblings were investigated for the presence of igm antibodies to coxsackie b virus serotypes 1-5 (cbv 1-5) with the aid of mu-antibody-capture radioimmunoassays. when a high cut-off value was used, 16 (46%) diabetic children and 16 (34%) siblings showed cbv-igm. of the siblings of diabetic patients positive for cbv-igm, 11 (44%) were cbv-igm-positive; the corresponding figure for the siblings of n ... | 1992 | 1314203 |
| persistent rubella-specific igm reactivity in the absence of recent primary rubella and rubella reinfection. | between two and seven sera from cases of persistent detection of rubella-specific igm for periods in excess of 2.5 months, but in the absence of recent primary rubella or rubella reinfection, were examined for rheumatoid factor, heterophile antibody, and igm reactivity against toxoplasma and a number of viruses. the relative avidity of the rubella-specific igg1 has been assessed in all the sera by two methods. none of the sera contained rheumatoid factor or heterophile antibody, nor did any cont ... | 1992 | 1314284 |
| early diagnosis of enteroviral meningitis by detection of specific igm antibodies with a solid-phase reverse immunosorbent test (sprist) and mu-capture eia. | a solid-phase reverse immunosorbent test (sprist) and a mu-capture enzyme immunosorbent assay (eia) for detection of enterovirus-specific igm antibodies were evaluated for enterovirus diagnosis of aseptic meningitis in 160 consecutive patients from whom enterovirus (11 different serotypes) were isolated in 64. in patients with an enterovirus isolate and/or four-fold titre rise in the complement fixation test (cft) for enterovirus, specific enterovirus igm antibodies were detected on the day of a ... | 1992 | 1314285 |
| lipopolysaccharide suppresses cytokine release from coxsackie virus-infected human monocytes. | infections by coxsackie virus b3 (cvb3) have been reported to be associated with an enhanced influx of mononuclear leukocytes into afflicted tissue. current evidence indicates that monocytes/macrophages are specifically involved in cvb3-induced myocarditis by maintaining a chronic inflammatory response. to examine susceptibility and reactivity to cvb3, freshly isolated human monocytes were exposed to various virus doses (0.1-10 moi) in the presence or absence of macrophage-activating lipopolysac ... | 1992 | 1314406 |
| virus infections complicating bone marrow transplantation. | virus infections account for considerable morbidity in bone marrow transplant (bmt) recipients. in all ages, members of the herpes virus group are the predominant pathogens. of these, cytomegalovirus is pre-eminent in being the most frequent cause of death due to infection associated with the transplant procedure. considerable effort is being invested in the development of preventative and therapeutic strategies to control this virus. other potentially life-threatening virus infections may be ac ... | 1992 | 1314724 |
| epstein-barr virus serology in the chronic fatigue syndrome. | the antibody profiles against epstein-barr virus were studied in 136 patients presenting with chronic fatigue syndromes. these profiles were compared with a panel of sera from blood donors. the patients exhibited higher titres in a combined assay for antibodies to the restricted (r) and diffuse (d) components of the early antigen complex than controls (p less than 0.001) but titres against these antigens were not useful on an individual patient basis. the patients who displayed elevated titres o ... | 1992 | 1314860 |
| increased frequency of coxsackie b virus igm in women with spontaneous abortion. | igm antibodies to coxsackie b virus serotypes 1-5 (cbv 1-5) were studied by radioimmunoassay (ria) of blood samples from women who had undergone a spontaneous abortion or given birth to a stillborn child. results were compared with those of controls comprising healthy pregnant women who had not suffered such experiences. among 48 women with abortions, 16 (33%) had cbv-igm, while among the controls only three of 37 (8%) had these antibodies, a statistically significant difference (p less than 0.0 ... | 1992 | 1314861 |
| [bacteriologic and virologic hygienic studies on waste water ponds]. | many problems arise with the overflow of wastewater after heavy rainfalls. therefore a research project was started to investigate the possibilities of biological treatment for such sporadically occurring sewage. in this paper a helophyte bed is compared with a simple wastewater pond regarding virological and bacteriological hygiene parameters. the number of coliphages is an evident indicator for enteroviruses and its enumeration can be done easily and safely. it is suggested that the kind of se ... | 1992 | 1315553 |
| towards identification of cis-acting elements involved in the replication of enterovirus and rhinovirus rnas: a proposal for the existence of trna-like terminal structures. | on the basis of a comparative analysis of published sequences, models for the secondary structure of the 3'-terminal [poly(a)-preceding] untranslated region of the entero- and rhinovirus rnas were worked out. the models for all these viruses share a common core element, but there are an extra enterovirus-specific element and still an additional element characteristic of a subset of enterovirus rnas. the two latter models were verified for poliovirus and coxsackievirus b genomes by testing with s ... | 1992 | 1315956 |
| [a preliminary epidemiological survey of acute hemorrhagic conjunctivitis in guangzhou during july to september in 1988]. | an epidemic of acute hemorrhagic conjunctivitis (ahc) occurred in guangzhou from july to september in 1988. the main clinical symptoms were feeling of foreign, smart pain, discharging ege, facial edema and conjunctival congestion. in some cases there were headache and fever. the patients recovered rapidly. epidemiologic feature were that the incubation period of ahc is short, rapid spread, wide prevalence and high attack rate. approximately 490000 cases of ahc are estimated to occur in guangzhou ... | 1992 | 1316235 |
| [antibody levels against coxsackie b viruses in patients with type 1 diabetes mellitus]. | in order to detect if the presence of interrelationship between coxsackie b viruses (cbv) and etiology of type 1 diabetes mellitus, antibodies to cbv by microneutralization test serotypes 1, 2, 3, 4, 5 and 6 were investigated in 37 sera obtained from patients with type 1 diabetes mellitus and 100 sera from healthy individuals as control group. no significant difference could found that neutralizing antibody levels to cbv serotypes between in sera obtained from patient with type 1 diabetes mellit ... | 1992 | 1316989 |
| the complete nucleotide sequence of a variant of coxsackievirus a24, an agent causing acute hemorrhagic conjunctivitis. | the complete nucleotide sequence was determined for the cdnas that represent the rna genome of the standard strain of a variant of coxsackievirus a24, the eh24/70, one of the agents causing acute hemorrhagic conjunctivitis. the genome is 7461 nucleotide long and is polyadenylated at the 3'-end terminus. following a 750-nucleotide 5'-noncoding region, there was a long open reading frame of 6642 nucleotides, which serve to encode a viral polyprotein consisting of 2214 amino acids. comparison of th ... | 1992 | 1317075 |