Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| murine sarcoma virus: the question of defectiveness. | infection of mouse and rat cells by the murine sarcoma virus (moloney isolate) showed two-hit kinetics for focus production in mouse cells but one-hit kinetics in rat cells. antiserum added to cultures after infection suppressed focus formation in mouse cells but not in rat cells. these studies suggest that, in rat cells infected with murine sarcoma virus, leukemina virus is not needed for focus formation and that these foci result from proliferation of the transformed rat cell; in mouse cells, ... | 1970 | 5435899 |
| the response of nxw-nzb f1 hybrid mice to a murine sarcoma virus. | 1970 | 5442811 | |
| virus-specified changes in the sugar-transport kinetics of rat embryo cells infected with murine sarcoma virus. | 1970 | 5449213 | |
| inhibition of oncogenicity of murine sarcoma virus (harvey) in mice by interferon. | 1970 | 5455139 | |
| effect of prolonged interferon treatment on mouse embryonic fibroblasts transformed by murine sarcoma virus. | 1970 | 5466001 | |
| radiological studies on the chronological relation between murine sarcoma virus infection and cell cycle. | 1970 | 5477330 | |
| immunological and pathological manifestations of murine sarcoma virus (moloney) infections. | 1970 | 5494580 | |
| properties of a murine sarcoma virus. | 1970 | 5538357 | |
| rescue of murine sarcoma virus genome in mixed cultures of 'non-producer' hamster tumour cells and helper virus carrier cell lines. | 1971 | 5542485 | |
| potentiation of murine sarcoma virus (harvey) (moloney) oncogenicity in lactic dehydrogenase-elevating virus-infected mice. | 1971 | 5554478 | |
| ultrastructure of giant cells in tumours induced in golden hamsters by murine sarcoma virus-harvey. | 1971 | 5559595 | |
| the age related responses of new zealand mice to a murine sarcoma virus. | 1971 | 5576664 | |
| [factors regulating the spontaneous regression of primary tumors induced by murine sarcoma virus, moloney strain, in mice (balb-c-cb-se). i. age at treatment and virus doses]. | 1967 | 5605880 | |
| some biologic, immunogenic, and morphologic effects in mice after infection with a murine sarcoma virus. i. biologic and immunogenic studies. | 1968 | 5648135 | |
| biological, pathological and physical characterization of a possible variant of a murine sarcoma virus (moloney). | 1968 | 5649158 | |
| increased oncogenicity of the murine sarcoma virus (moloney) by co-infection with murine leukemia viruses. | 1968 | 5658439 | |
| pathogenesis of a rhabdomyosarcoma (undifferentiated type) in rats induced by a murine sarcoma virus (moloney). | 1968 | 5658448 | |
| presence in murine sarcoma virus stocks of a 3d component which alone initiates cellular conversion. | 1968 | 5667074 | |
| effects of antilymphocyte serum on induction of tumours and leukemia by murine sarcoma virus. | 1968 | 5686746 | |
| some biologic, immunogenic, and morphologic effects in mice after infection with a murine sarcoma virus. ii. morphologic studies. | 1968 | 5689654 | |
| transformation by murine sarcoma virus: fixation (deoxyribonucleic acid synthesis) and development. | transformation of rat embryo cells by murine sarcoma virus (msv) was contingent upon synthesis of deoxyribonucleic acid (dna) during the first 12 hr of infection. inhibition of dna synthesis by thymidine (20 mm) or cytosine arabinoside (0.1 mm) resulted in the protection of cells from transformation by msv. transient suppression of dna synthesis prior to infection or after a 12-hr delay had little effect on subsequent transformation, emphasizing the critical time period in in which dna synthesis ... | 1968 | 5701824 |
| enhancing effect on tumour growth of humoral antibodies against tumour specific transplantation antigens in tumours induced by murine sarcoma virus(harvey). | 1968 | 5722149 | |
| biological and serological properties of viral particles from a nonproducer rat neoplasm induced by murine sarcoma virus (moloney). | the viral particles present in a nonproducer rat neoplasm induced by murine sarcoma virus (msv) moloney isolate, as detected by electron microscopy, were found to be biologically active on normal kidney cells of random-bred osborne-mendel rats. the virus is designated here as msv (0). msv (0) differs from other pseudotypes of msv in its host range, antigenicity, and interference pattern. | 1968 | 5725318 |
| further studies on the murine sarcoma virus (harvey) complex--isolation of a murine leukaemia virus. | 1968 | 5741995 | |
| quantitative dose-response relationships of murine sarcoma virus (moloney) in balb/c mice. | 1969 | 5765223 | |
| increased tumorigenesis of murine sarcoma virus (moloney) by coinfection with rauscher virus or by treatment with antilymphocyte serum. | 1969 | 5781092 | |
| requirements for rna and dna synthesis in the transformation of mouse embryo cells by murine sarcoma virus (moloney). | 1969 | 5786165 | |
| rescue in vivo in adult mice of murine sarcoma virus (msv) from a nonproducer rat neoplasm. | 1969 | 5791815 | |
| viral infection across species barriers: reversible alteration of murine sarcoma virus for growth in cat cells. | infection of cat embryo cells by a centrifugally induced aggregate of murine sarcoma virus and feline leukemia virus gave rise to a defective, focus-forming virus which propagated in cat cells, but not in mouse cells. this virus, apparently enveloped with a feline leukemia virus coat, was later subjected to aggregation with murine leukemia virus, whereupon it regained the capacity for growth in mouse cells. | 1969 | 5793978 |
| karyological analysis of mouse and rat embryo cells transformed by murine sarcoma virus (harvey). | 1969 | 5801815 | |
| myo-inositol influences release and inactivation of murine sarcoma virus from desiccated transformed mouse embryo cells. | 1969 | 5812421 | |
| plasmacytomas occurring in the bones of rats injected intracerebrally with murine sarcoma virus (msv), moloney' strain. preliminary report. | 1966 | 5985024 | |
| the p21 ras c-terminus is required for transformation and membrane association. | the harvey murine sarcoma virus (ha-musv) transforming gene, v-rash, encodes a 21,000 molecular weight protein (p21) that is closely related to the p21 proteins encoded by the cellular transforming genes of the ras gene family. the primary translation product (prop21), which is found in the cytosol, undergoes posttranslational modification and the mature protein subsequently becomes associated with the inner surface of the plasma membrane and binds lipid tightly. the p21 proteins have the capaci ... | 1984 | 6087162 |
| an altered dna sequence encompassing the ras gene of harvey murine sarcoma virus. | the dna fragment encompassing the ras gene of harvey murine sarcoma virus was sequenced and assigned the coding region of a transforming protein, p21, to the sequence. examination of nucleotide sequence, taken together with the result of analysis of the ras mrnas (1), has revealed that p21 is encoded from a continuous coding region starting with the 5' proximal initiation codon but not a processed protein. however, there were found several differences between the sequence published by dhar et. a ... | 1984 | 6087292 |
| murine retrovirus pr65gag forms a 130k dimer in the absence of disulfide reducing agents. | gazdar-murine sarcoma virus (gz-msv) particles, obtained from tissue culture fluids of chronically infected htg-2 hamster cells are immature in morphology and contain uncleaved pr65gag as the predominant protein (greater than 95% coomassie blue stain) (a. pinter and e. deharven, 1979, virology 99, 103-110; y. yoshinaka and r. b. luftig, 1982, virology 118, 380-388). when gz-msv particles are disrupted in 1% sodium dodecyl sulfate (sds) and then analyzed by sds-polyacrylamide gel electrophoresis ... | 1984 | 6087546 |
| [isolation and characterization of retroviruses expressed in murine osteosarcomas induced by 90sr]. | the induction of osteosarcomas with 90sr in cf1 mice is associated with the expression of ecotropic type-c rna viruses devoid of sarcomatogenic activity. in contrast, the fbr murine osteosarcoma virus complex, isolated from a 90 sr-induced osteosarcoma of a x/gf mouse [m. finkel et al. (1)], causes the rapid appearance of osteosarcomas in newborn mice and transforms fibroblasts in vitro. the transforming capacity of fbr murine sarcoma virus has been associated as an oncogene homologous to v-fos. | 1984 | 6088003 |
| homologies between a brain-specific identifier (id) sequence and regions of harvey murine sarcoma virus and rous sarcoma virus genomes. putative role of identifier sequences in the tissue specificity of malignant transformation by rna tumor viruses. | as a step toward understanding of the tissue specificity of cellular transformation by rna tumor viruses were looked for the presence of a putative brain specific regulatory (identifier) sequence (c82b) in the genome of various oncornaviruses. the genomes of harvey murine sarcoma virus and rous sarcoma virus contain sequences flanking the viral oncogenes with greater than 80% and greater than 60% homology to c82b, respectively. we suggest that identifier sequences acquired by oncoviruses may det ... | 1984 | 6088289 |
| expression of normal and transforming h-ras genes in escherichia coli and purification of their encoded p21 proteins. | the h-ras gene of the balb murine sarcoma virus (balb-msv) was placed under the transcriptional control of the tightly regulated pl promoter of bacteriophage lambda in the expression vectors pev-vrf-1 and prc23. upon derepression of the pl promoter, large amounts (10-20% of total cellular protein) of the h-ras gene product p21 are synthesized in escherichia coli. we constructed three h-ras gene expression vectors, designated pjcl-h5, pjcl-e30, and pjcl-33. pjcl-h5 directs the synthesis of p21, a ... | 1984 | 6089191 |
| sequences in the long terminal repeats of the moloney murine sarcoma virus-124 genome which control transforming gene function. | the role of long terminal repeat (ltr) sequences in the efficient expression of moloney murine sarcoma virus (msv-124) transforming gene function was investigated. recombinant plasmids containing a single ltr positioned 3' of v-mos were subjected to sequential deletions, and the relative transforming efficiency of these recombinants was analyzed in the nih/3t3 transfection assay. recombinants lacking caat, tata, and poly(a) signals within the ltr were able to transform with an efficiency compara ... | 1984 | 6089418 |
| modulation of enhancer activity by the hormone responsive regulatory element from mouse mammary tumor virus. | addition of the transcriptional enhancers present in the u3 region of the harvey murine sarcoma virus (hamusv) long terminal repeat (ltr) to recombinant chimeras in which the hamusv transforming gene (ha-v-ras) is expressed from the mouse mammary tumor virus (mmtv) promoter increases the ability of the mmtv v-ras chimeras to transform mouse fibroblasts in culture 50- to 100-fold. significant stimulation of transfection efficiency occurs only when glucocorticoids are present in the culture medium ... | 1984 | 6090130 |
| flat revertants derived from kirsten murine sarcoma virus-transformed cells produce transforming growth factors. | two flat cellular revertant cell lines, f-2 and c-11, which were originally selected from the dt line of kirsten murine sarcoma virus (ki-musv)-transformed nih/3t3 cells, were examined for the production of transforming growth factors (tgfs). the revertant cells fail to grow in semisolid medium as colonies and exhibit a markedly reduced level of tumorigenicity in nude mice, although they are known to express high levels of p21ras, the product of the kirsten sarcoma virus oncogene, ras, and they ... | 1984 | 6090475 |
| genesis of kirsten murine sarcoma virus: sequence analysis reveals recombination points and potential leukaemogenic determinant on parental leukaemia virus genome. | the genome of kirsten murine sarcoma virus was formed by recombination between kirsten murine leukaemia virus sequences, and rat sequences derived from a retrovirus-like '30s' (vl30) genetic element encompassing the kras oncogene. using cloned dnas we have determined the nucleotide sequences of the long terminal repeats and adjacent regions, extending across the points of recombination on the sarcoma and leukaemia virus genomes. our results suggest that discrete regions of homology and other cry ... | 1984 | 6091040 |
| use of the mouse mammary tumor virus long terminal repeat to promote steroid-inducible expression of v-mos. | we used the mouse mammary tumor virus long terminal repeat to promote dexamethasone-regulated expression of the moloney murine sarcoma virus (m-msv) transforming gene, v-mos. a recombinant dna vector containing the mouse mammary tumor virus long terminal repeat fused to the m-msv 124 v-mos gene was cotransfected with a plasmid containing the herpes simplex virus thymidine kinase gene (tk) into 3t3tk- cells. individual clones of cells which grew in hypoxanthine-aminopterin-thymidine medium were t ... | 1984 | 6092668 |
| metabolic turnover of human c-rash p21 protein of ej bladder carcinoma and its normal cellular and viral homologs. | the ej bladder carcinoma oncogene is activated by a point mutation in the c-rash proto-oncogene at the 12th amino acid codon. in an attempt to understand the mechanism of oncogenic activation, a comparative study was undertaken to examine the metabolic turnover and subcellular localization of the p21 protein encoded by the ej oncogene, the viral oncogene, and its normal cellular homolog. pulse-labeling experiments indicated that both c-ras p21 proteins were synthesized by a very similar pathway, ... | 1984 | 6092927 |
| biological properties of human c-ha-ras1 genes mutated at codon 12. | vertebrate genomes contain proto-oncogenes whose enhanced expression or alteration by mutation seems to be involved in the development of naturally occurring tumours. these activated genes, usually assayed by their ability to induce the malignant transformation of nih 3t3 cells, are frequently related to the ras oncogene of harvey (ha-ras) or kirsten (ki-ras) murine sarcoma viruses, or a third member of this family (n-ras). activation involves point mutation which often affect codon 12 (refs 16- ... | 1984 | 6092966 |
| expression of the onc gene of the kirsten murine sarcoma virus in differentiated rat thyroid epithelial cell lines. | a systematic study has been performed using a series of differentiated rat thyroid epithelial cell lines either uninfected or infected with kirsten murine sarcoma virus (kimsv), to determine the levels of the p21 product of the v-ras-ki oncogene in transformed and normal cell lines. the p21 levels have been assayed by sds-polyacrylamide gel electrophoresis of immunoprecipitates of 35s-labelled cell extracts and by a gdp binding assay. all cell lines analysed showed a significant increase in the ... | 1984 | 6094710 |
| the long terminal repeat of an endogenous intracisternal a-particle gene functions as a promoter when introduced into eucaryotic cells by transfection. | we describe experiments designed to determine whether an endogenous intracisternal a-particle (iap) gene randomly selected from a mouse embryo library has the potential to be transcriptionally active. assays for iap gene transcription were done with permanently transformed rat cells and transiently transfected monkey and mouse cells. the rat cells, which had integrated iap gene copies, contained iap rna. a start site within the iap 5' long terminal repeat (ltr) was localized by s1 mapping. the p ... | 1984 | 6095042 |
| introduction of a selectable gene into different animal tissue by a retrovirus recombinant vector. | the potential use of retrovirus vectors to transduce foreign genetic information into cells of different tissues of an animal was explored by introducing a recombinant genome carrying the eco gpt gene into postimplantation mouse embryos. to obviate the need for preparing concentrated virus stocks, psi 2-2-5 cells producing the replication-defective murine sarcoma virus (msv)-gpt virus were microinjected directly into embryos. the psi 2-2-5 cells were mixed with cells producing replication-compet ... | 1984 | 6095271 |
| harvey murine sarcoma virus p21 ras protein: biological and biochemical significance of the cysteine nearest the carboxy terminus. | previous studies of premature chain termination mutants and in frame deletion mutants of the p21 ras transforming protein encoded by the transforming gene of harvey murine sarcoma virus (ha-musv) have suggested that the c terminus is required for cellular transformation, lipid binding, and membrane localization. we have now further characterized the post-translational processing of these mutants and have also studied two c-terminal v-rash point mutants: one encodes serine in place of cysteine-18 ... | 1984 | 6096132 |
| the gag-mos hybrid protein of ts110 moloney murine sarcoma virus: variation of gene expression with temperature. | a nrk cell clone (6m2 cells) infected with ts110 moloney murine sarcoma virus (musv) produce a gag-mos protein, p85gag-mos, and a truncated gag protein of mr 58,000d termed p58gag. the gag-mos protein is produced from a 3.5-kb mrna whereas the gag protein is made from a 4.0-kb mrna. it has been proposed that the 3.5-kb rna is produced from the 4.0-kb rna by a splicing mechanism (r. p. junghans, e. c. murphy, jr., and r. b. arlinghaus (1982) j. mol. biol. 161, 229-255). the results presented here ... | 1984 | 6097031 |
| brain tumors in hamsters induced by murine sarcoma virus (moloney). | murine sarcoma virus, cs-moloney substrain, was inoculated intracranially into 2 litters of newborn syrian hamsters within 24 h of birth. seven of 12 hamsters which survived more than 30 days developed brain tumors in the cerebral cortex 104 to 153 days, 139 days on the average, after the virus inoculation. the tumors consisted of spindle-shaped, round or polygonal astrocytes which showed a positive reaction for glial fibrillary acidic protein by the immunoperoxidase method. | 1984 | 6097099 |
| efficient expression of cloned complementary dnas for secretory proteins after injection into xenopus oocytes. | cloned complementary dnas encoding chicken ovalbumin, chicken prelysozyme and calf preprochymosin, prochymosin and chymosin were inserted downstream from various viral promoters in modified recombinant "shuttle" vectors. microinjection of the ovalbumin, prelysozyme and preprochymosin constructs into the nuclei of xenopus laevis oocytes resulted in the synthesis, segregation in membranes and secretion into the extracellular medium of ovalbumin, lysozyme and prochymosin, respectively. judging from ... | 1984 | 6098686 |
| adoptive t cell immunotherapy of msv-induced tumours in nude mice. part i. biology of tumour regression and recurrence. | the induction of immunity to progressively growing murine sarcoma virus (msv) tumours in nude (nu/nu) mice by reconstitution with immune t cells from syngeneic (+/+) donors has been studied. whole spleen cell preparations served as the source of immune t cells. transfer of immune, but not of normal, spleen cells resulted in partial or apparently complete regression of primary tumours and a related moderate to considerable extension of survival time. the dose, the time in days between immunizatio ... | 1983 | 6100803 |
| adoptive t cell immunotherapy of msv-induced tumours in nude mice. part ii. sequential analysis of serum immune complexes and blocking activity in reconstituted mice in relation to tumour biology. | in seven separate experiments, nude (nu/nu) mice carrying established murine sarcoma virus (msv) tumours were reconstituted with syngeneic (+/+) immune splenic t cells. these immune protected mice were randomly divided to provide smaller groups for serial exsanguination. at various time points mice were individually bled and cic concentration and blocking activity of each individual serum was determined. control sera were obtained from nu/nu and adult +/+ mice inoculated with tumour cells only, ... | 1983 | 6100804 |
| high-level expression of oncogenes in escherichia coli. | a plasmid, pjl6, was constructed that contains a unique cla i site 12 codons beyond the bacteriophage lambda cii gene initiation codon, as well as an adjacent unique hind iii site. these sites allowed us to fuse the sequences from the avian myelocytomatosis virus (mc29) v-myc gene, the avian myeloblastosis virus (amv) v-myb gene, and the harvey murine sarcoma virus (ha-musv) v-ras gene to the amino-terminal portion of the cii gene. transcription of the hybrid genes is controlled from the lambda ... | 1983 | 6101025 |
| mouse cells contain two distinct ras gene mrna species that can be translated into a p21 onc protein. | the kirsten (ki) and harvey (ha) strains of murine sarcoma virus encode a 21,000-dalton protein (p21 ras) which is the product of the transforming gene of these viruses. normal cells express low levels of p21 ras encoded by cellular genes (ki-ras and ha-ras) homologous to the ki and ha murine sarcoma virus transformation genes. a bone marrow-derived mouse cell line, 416b, has been shown to express unusually high levels of p21 ras. in this manuscript, we investigated the molecular biology of p21 ... | 1982 | 6131379 |
| demonstration of virus particles in moloney murine sarcoma virus-induced periosteal bone in mice. | balb/c mice were inoculated intramuscularly with moloney murine sarcoma virus in one of the hind legs. this led to the rapid development of a regressive sarcoma and also to the proliferation and osteogenic differentiation of cells in the adjacent periosteum. examination of the tissues by transmission electron microscopy revealed the presence of type a and c virus particles within the sarcoma cells as well as within the cells of the newly formed bone. extracellular type c virus particles were for ... | 1984 | 6147919 |
| antigenic specificity of the cytolytic t lymphocyte (ctl) response to murine sarcoma virus-induced tumors. ii. analysis of the clonal progeny of ctl precursors stimulated in vitro with syngeneic tumor cells. | 1980 | 6154076 | |
| leukocyte adherence inhibition response to murine sarcoma virus-induced tumors. i. cell requirements, specificity, lack of h-2 restriction, and role of antibody. | 1980 | 6154099 | |
| characterization of viral polyproteins in cells transformed and producing moloney murine sarcoma virus-124. | the viral proteins of moloney murine sarcoma virus-transformed mouse cells (g8-124), that overproduce the sarcoma virus relative to helper leukemia virus, were examined by immunoprecipitation, sizing by gel electrophoresis and peptide mapping. using antisera to the viral core proteins, a p10-deficient core polyprotein of 63 000 daltons (p63gag) was detected which was found to be a product of the musv-124 genome. helper virus proteins pr67gag, gpr85gag, pr200gag-pol, and gpr83env were also detect ... | 1980 | 6156703 |
| antigenic specificity of the cytolytic t lymphocyte response to murine sarcoma virus-induced tumors. iii. characterization of cytolytic t lymphocyte clones specific for moloney leukemia virus-associated cell surface antigens. | cytolytic t lymphocyte (ctl) clones specific for moloney leukemia virus (molv)-derived tumor cells were generated by placing limiting numbers of c57bl/6 responder cells into mixed leukocyte-tumor cell microcultures. under appropriate conditions (presence of stimulating tumor cells, accessory cells, and t cell growth factor), such cloned ctl could readily be expanded to provide large numbers of homogeneous, highly cytolytic ctl populations for further characterization. using four target-cell type ... | 1980 | 6159445 |
| interferon inhibits transformation by murine sarcoma viruses before integration of provirus. | neoplastic transformation by c-type retroviruses requires synthesis of a dna copy (the provirus) of the rna genome and its integration into the host cell dna. we have previously shown that interferon (ifn) can stably prevent transformation of murine fibroblasts by the kirsten strain of murine sarcoma virus (kimsv), a murine leukaemia virus (mlv). a series of cell clones (ifn clones), isolated in the presence of ifn (10(4) u ml-1) from cultures of nih-3t3 cells which had been treated with ifn, an ... | 1980 | 6159546 |
| detection of a recombinant murine leukemia virus-related glycoprotein on virus-negative thymoma cells. | x-irradiation of outbred swiss mice resulted in the development of virus-free thymomas. when put in culture, a lymphoblastic cell line (nixt) expressed neither particles nor infectious virus but supported the growth of pure ecotropic murine leukemia viruses (mulvs) without generating any envelope recombinant (rm) mulv in more than 20 months of culture. these cells did not support the growth of rm-mulvs and completely excluded the entry of all rm-mulv pseudotypes of murine sarcoma virus, suggesti ... | 1981 | 6165021 |
| regulation of cell-mediated immunologic reactivity to moloney murine sarcoma virus-induced tumors. ii. nature of blocking and unblocking factors in serum. | serum blocking factors, detected in sera of inbred cba and balb/c mice with progressing virus-induced tumors by their interference with leukocyte-adherence inhibition (lai) with the use of syngeneic leukocytes, were found to be strain related: no blocking occurred with allogeneic leukocytes. furthermore, the unblocking factors of regressor serum were also strain related: no unblocking of allogeneic progressor serum was observed. there was no requirement for h-2 compatibility between leukocytes a ... | 1981 | 6165853 |
| interferon effect on collagen and fibronectin distribution in the extracellular matrix of murine sarcoma virus-transformed cells. | the transformation of murine balb/c embryonic fibroblasts by murine sarcoma virus, moloney strain, followed by prolonged treatment with murine interferon, resulted in the appearance of a new cell population (msv-if+). these msv-if+ cells are characterized by the recovery of a normal phenotype, contact inhibition, and lack of colony formation in agar. this phenotypic change of the msv-if+ cells is associated to the neosynthesis of a dense fibrous matrix beyond the cell periphery. ultrastructural ... | 1981 | 6167350 |
| purified low-molecular-weight protein kinase from murine sarcoma virus particles catalyzes tyrosine phosphorylation endogenously but phosphorylates cellular proteins at serine. | the low-molecular-weight (lmw) protein kinase associated with high-titer murine sarcoma virions have been extensively purified by ammonium sulfate fractionation. bio-gel p-100 gel filtration, deae-cellulose and carboxymethyl cellulose chromatography. the purified enzyme migrates as a 16k polypeptide in polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. the enzyme catalyzes phosphotransfer with atp as a phosphate donor to various exogenously added proteins as acceptors; ... | 1981 | 6168778 |
| further studies on anti-tumor activity of adenovirus fibre protein on murine sarcoma tumours. | the present study demonstrates that subcutaneous injection of purified adenovirus 12 fibre protein (fp) to 4-week-old balb/c mice, 4, 2 and 1 day(s) before intramuscular injection of murine sarcoma virus (msv-m) or 2 and 1 day before and 1 day after msv-m injection causes significant inhibition of tumour growth (p less than 0.001). the evidence suggests that treatment with fp leading to tumour inhibition is due to neither an interferon mechanism nor to inhibition of virus multiplication of fp in ... | 1981 | 6169787 |
| in vitro proteolytic cleavage of gazdar murine sarcoma virus p65gag. | moloney murine leukemia virus, disrupted in concentrations of 0.1 to 0.5% nonidet p-40, catalyzed the cleavage of p65, the gag gene polyprotein of the gazdar strain of murine sarcoma virus, into polypeptides with sizes and antigenic determinants of murine leukemia virus-specified p30, p15, pp12, and p10. cleavage performed in the presence of 0.15% nonidet p-40 in water yielded polypeptides of approximately 40,000 (p40) and 25,000 (p25) mr. in vitro cleavage performed in a buffered solution conta ... | 1981 | 6169847 |
| complete nucleotide sequence and organization of the moloney murine sarcoma virus genome. | the complete nucleotide sequence of a mammalian transforming retrovirus. moloney murine sarcoma virus, has been determined. msv, recombinant virus derived of helper viral and cellular sequences, possesses termini resembling prokaryotic transposable elements. the viral genome has the coding capacity for the moloney murine leukemia virus gag gene product and contains large deletions in pol and env genes. a large open reading frame encompassing its cell-derived sequences codes for its putative tran ... | 1981 | 6170110 |
| partial reversion of the transformed phenotype of murine sarcoma virus-transformed cells in the presence of interferon: a possible mechanism for the anti-tumour effect of interferon. | the effect of interferon (ifn) on a series of cellular properties which characterize the transformed state was studied, using normal mouse fibroblasts (both established lines and embryo cells) and cloned derivatives transformed by murine sarcoma virus. it was found that the transformed cells behaved in a more normal fashion in the presence of ifn. this was indicated by reduction in saturation density, decreased dna synthesis in crowded cultures, decreased formation of foci on monolayers of norma ... | 1981 | 6171576 |
| nucleotide sequence of the genome of a murine sarcoma virus. | 1981 | 6173134 | |
| rapid syncytium formation induced by moloney murine sarcoma virus in 3t3/nih cells and its delay by mouse interferon. | moloney murine sarcoma virus (msv) clone 124 was found to induce rapid syncytium formation upon infection of 3t3/nih at a high multiplicity of infection. this effect became apparent, by light microscopy, within 1 to 2 hours, whereas by scanning electron microscopy, clusters of 4 to 25 cells were seen in their initial steps of syncytium formation within 20 to 30 minutes after virus addition. it appeared that the cell fusion was initiated by connection between the cells through virus bound to thei ... | 1981 | 6174107 |
| use of hybridoma-resident variant cell lines to study h-2db/fmr-specific cytotoxic t cells. | an h-2db heterozygous tumor cell line and a variant subclone bearing a mutant gene product were used to analyze the h-2db specificity of cytotoxic t lymphocytes (ctl) generated during a moloney murine sarcoma virus (msv) infection. when the mutant cells were used as targets for msv-specific ctl, the amount of cell lysis, compared with that seen with the nonmutant parental cells, was drastically decreased. however, cells of the mutant clones remained susceptible to allogeneic ctl specific for the ... | 1981 | 6174439 |
| diverse effects: augmentation, inhibition, and non-efficacy of 12-o-tetradecanoylphorbol-13-acetate (tpa) on retrovirus genome expression in vivo and in vitro. | the action of 12-o-tetradecanoylphorbol-13-acetate (tpa) on several retrovirus-related functions was investigated in four virus-host cell systems. the following effects were recorded: (i) in stu-mice, infected with the friend virus complex (friend) murine leukaemia virus/friend spleen focus forming virus) and treated with tpa (50 ng/g) for one week prior to infection, the number of spleen foci increased 5-fold over the control. (ii) addition of tpa (0.04 to 40 ng/ml) to virus-producing cell syst ... | 1982 | 6177439 |
| carcinogen- and radiation-transformed c3h 10t1/2 cells contain rnas homologous to the long terminal repeat sequence of a murine leukemia virus. | carcinogen- or radiation-transformed c3h 10t1/2 murine fibroblasts transcribe a set of poly(a)+rnas that contain sequences homologous to the long terminal repeat (ltr) sequence of moloney murine sarcoma virus. these ltr-containing rnas consist of a series of discrete bands ranging in size from about 38 to 18 s. the higher molecular weight molecules (30-38 s) in this set of rnas also contain sequences homologous to the gag, pol, and env genes of a murine leukemia virus. a 24s rna contains sequenc ... | 1982 | 6178111 |
| p65 of gazdar murine sarcoma viruses contains antigenic determinants from all four of the murine leukemia virus (mulv) gag polypeptides (p15, p12, p30, and p10) and can be cleaved in vitro by the mulv proteolytic activity. | 1982 | 6178211 | |
| reorganization of the cytoskeleton by interferon in msv-transformed cells. | beta and gamma interferons were found to enhance the organization of microfilaments, intermediate filaments, and fibronectin in the murine sarcoma virus (msv)-transformed cell line. furthermore, both interferons increased the number of intermediate-type junctions. sodium butyrate was also found to act in a similar way and, when associated with interferon, an additive effect was observed. in the cell line, cultured for many passages in the presence of beta interferon (msv-if+), the cytoskeletal n ... | 1981 | 6180063 |
| identification of proteins encoded by the gazdar murine sarcoma virus genome by in vitro translation and comparison with moloney murine sarcoma virus 124. | the gene products of gazdar murine sarcoma virus (gz-musv) were identified by in vitro translation of gz-musv virion rna. an overlapping set of proteins with approximate molecular weights of 37,000 (37k), 33k, 24k, and 18k were synthesized from the transforming gene of gz-musv, v-mosgz. in addition, gz-musv-specific rna directed the in vitro synthesis of a 62k gag gene protein and a 37.5k env gene-related product. the gz-musv-specific in vitro translation products were compared with the in vitro ... | 1982 | 6180181 |
| viral-versus non-viral-induced yolk sac tumors in the rat. | yolk sac tumors were induced in fetectomized rats by displacement of the visceral yolk sac outside the uterus with or without injection of murine sarcoma virus (msv) into the placenta. some of the animals were immunized with msv prior to surgery. the biological, morphological and immunological characteristics of the tumors which develop in animals either injected or not with virus are compared. the results indicate that msv inoculation into the placenta increases the tumor incidence, shortens th ... | 1982 | 6181488 |
| role of accessory cells in the induction of a secondary cytotoxic response to moloney murine sarcoma virus-induced tumors. | the role of ia-positive accessory cells in the generation of a secondary cytotoxic response to tumor-associated antigens induced by moloney murine sarcoma virus (m-msv) was evaluated. spleen cells from m-msv-immune a.tl mice, depleted of accessory cells by anti-iak serum plus c treatment and stimulated in secondary mixed leukocyte tumor cell culture (mltc) with syngeneic ia-negative a6atl moloney leukemic cells, failed to generate virus-specific cytotoxic t lymphocytes (ctl). ctl generation in i ... | 1983 | 6185589 |
| specific antigrowth effect of interferon on mouse cells transformed by murine sarcoma virus. | the growth rate of nih/3t3 mouse fibroblasts transformed by the moloney strain of murine sarcoma virus was investigated following interferon (ifn) treatment. these cells were found to be sensitive to the antigrowth effect of ifn as indicated by a slower growth rate in its presence. the effect was most efficiently expressed when cells were grown at low serum concentrations, e.g., 2.5%. likewise, ifn treatment caused a reduction in the rate of dna synthesis as measured by [3h]thymidine incorporati ... | 1983 | 6186372 |
| [role of interferon in the phenotypic reversion of transformed cells: loss of malignancy]. | transformation of cells by oncogenic rna viruses is related to a number of significant changes in cell morphology and functions. the most common features of transformed cells are essentially the decrease of cell adhesiveness and the loss of contact inhibition. these changes are probably associated with an alteration of the cytoskeleton together with a marked modification in the number and distribution of membrane and extracellular matrix components. balb/c embryonic fibroblasts transformed by mu ... | 1983 | 6190122 |
| the mechanism of interferon effect on cell transformation by murine sarcoma virus. | mouse interferon (ifn) was found to inhibit murine sarcoma virus (msv)-induced neoplastic transformation of normal rat kidney (nrk) cells. this effect was observed upon examining the formation of foci of morphologically altered cells and colonies of anchorage-independent cells. ifn had no cytotoxic effect on msv-transformed nrk cells, nor on their focus or colony-forming ability. it was therefore apparent that its inhibitory effect was directed against the viral role in cell transformation. in a ... | 1983 | 6190765 |
| inhibition of retrovirus dna supercoiling in interferon-treated cells. | the effect of interferon (ifn) on the cytoplasmic synthesis of murine sarcoma virus dna, its transport to the host nucleus, and its integration into the cellular genome were investigated. for this purpose, at various intervals after infection. dna was extracted from the cytoplasmic fraction, nuclear hirt supernatant, and chromosomal dna pellet. the relative amount of viral dna was estimated by c0t kinetics analysis of hybridization to murine sarcoma virus-specific [3h]cdna. ifn was found to dela ... | 1983 | 6193285 |
| presence of a kirsten murine sarcoma virus ras oncogene in cells transformed by 3-methylcholanthrene. | oncogenes have previously been reported in the dnas of mouse fibroblast lines which had become transformed after in vitro exposure to the carcinogen 3-methylcholanthrene. these oncogenes are now shown to be versions of the cellular kirsten ras gene and are therefore homologous to oncogenes detected in a variety of human tumor dnas. | 1983 | 6197628 |
| fbr murine osteosarcoma virus. i. molecular analysis and characterization of a 75,000-da gag-fos fusion product. | the fbr murine osteosarcoma virus complex induces bone tumors with a similar latency and pathology to those induced by the fbj virus complex. fbr murine sarcoma virus ( fbr -msv) has been isolated from its helper virus(es) by the establishment of transformed nonproducer cells. these cells were found to express a 75,000-da protein (p75) which was antigenically related to the p55 oncogene product of the fbj murine osteosarcoma virus ( fbj -msv). p75 also contained antigenic determinants of murine ... | 1984 | 6203214 |
| tyrosyl kinases acquired from anchorage-independent cells by a membrane-enveloped virus. | tyrosyl kinase activity in vesicular stomatitis virus (vsv) acquired from host cells that differ in morphology was investigated. vsv grown in baby hamster kidney (bhk) cells with rounded morphology and a high efficiency of colony formation in soft agar (rous sarcoma virus [rsv]-transformed and suspension bhk cells) was compared with vsv grown in bhk cells with a flattened morphology and lower efficiency of colony formation in soft agar (rsv-infected revertant and control bhk cells). tyrosyl kina ... | 1984 | 6206078 |
| transformation by murine sarcoma virus alters the sensitivity of clonal cells derived from nih/3t3 mouse fibroblasts to interferon. | in contrast to normal clonal cells (a5) derived from nih/3t3 mouse fibroblasts, another clone (a10) derived from the same source was found to be resistant to the anti-lytic-virus activity of ifn and to be deficient in the induction of (2'-5') oligoadenylate synthetase (2-5a synthetase) by ifn. following infection of either a5 or a10 cells with moloney murine sarcoma virus (msv), a few transformed colonies were isolated, expanded, and tested for their sensitivity to ifn. it is clearly demonstrate ... | 1984 | 6206644 |
| induction of gp70-specific suppressor t-cells in mice inoculated with virus-producing tumor cells. | inoculation of balb/c mice with syngeneic, murine leukemia virus (mulv)-producing (murine sarcoma virus-transformed) balb/3t3 tumor cells resulted in diminution of alloreactivity as measured in the mixed leukocyte culture (mlc) reaction. cells that suppressed this response were identified in the spleens of tumor-bearing mice 10--14 days after inoculation. the suppressor cells were adherent, radiosensitive t-lymphocytes of the lyt 1-, 2, 3+ phenotype. mice inoculated with gross mulv (g-mulv)-prod ... | 1982 | 6214655 |
| dihydrocytochalasin b disorganizes actin cytoarchitecture and inhibits initiation of dna synthesis in 3t3 cells. | dihydrocytochalasin b (h2cb) disrupts the actin structure of swiss/3t3 mouse fibroblasts and inhibits the ability of serum growth factors to stimulate dna synthesis in quiescent cultures. low doses of h2cb (2-10 x 10(-7) m) added to serum-arrested cells reversibly block initiation of dna synthesis by serum; by epidermal growth factor and insulin; or by epidermal growth factor, fibroblast growth factor and insulin. h2cb is effective only when added to cells within 8-10 hr after stimulation. low d ... | 1982 | 6215122 |
| genetic control of sensitivity to moloney leukemia virus in mice. vi. involvement of virus-specific t helper cells collaborating with b cells. | t cell responses to moloney virus involve cytolytic and helper lymphocytes. in contrast to specific cytolytic t lymphocytes, few studies have been devoted to the characteristics of helper t cells for antibody production. the present experiments describe an assay for moloney virus-specific help for b cells using dinitrophenylated virus. this method, using the moloney virus as a carrier in a hapten-carrier system, allows to definition of the specific helper function of antibody responses. t helper ... | 1982 | 6216112 |
| clonal analysis of the t lymphocyte response to murine sarcoma virus-induced tumors. | 1982 | 6217605 | |
| t cell-mediated immunity to oncornavirus-induced tumors. i. ly phenotype of precursor and effector cytolytic t lymphocytes. | regression of tumor induced by murine sarcoma virus (msv) is accompanied by the formation of specific cytolytic t lymphocytes (ctl). selection of t-cells sets by ly phenotype determination allows separation of t sets involved in the cytolytic reaction. after msv inoculation we demonstrate that a) ly123+ cells contain precursors of ctl, b) direct cytolysis is mainly mediated by ly23 cells, 3) cytolytic memory is divisible into "early" memory, carried by ly23 cells, and "late" memory, which revert ... | 1980 | 6243332 |
| moloney murine sarcoma virus oncogenesis in t-lymphocyte-deprived mice: biologic and immunologic studies. | 1980 | 6243378 | |
| envelope properties of murine sarcoma virus-zero (msv-z). | 1980 | 6243828 | |
| biological and molecular biological characterization of the virus progeny from transformed clones musv-124 and musv-349: evidence for mulv-specific nucleotide sequences in the momusv size class of rna from momusv-124. | the genetic information of momusv-349 and momusv-124, two clones of productively transformed tb cells, was distributed between two size classes of rna (mol. wt. 2.9 x 10(6)) in the proportions of 5:1. some preparations of momusv-124 lacked the large rna. the virions produced by both clones also contained all the nucleotide sequences of moloney leukaemia virus and the ratio of musv:mulv produced by the two clones differed markedly. the distribution of the sequences specific for moloney murine leu ... | 1980 | 6245178 |
| structure of murine sarcoma virus dna replicative intermediates synthesized in vitro. | moloney murine sarcoma virions synthesize discrete dna products in vitro which closely resemble those found in vivo shortly after infection. these in vitro products have been isolated by electrophoresis and mapped with restriction endonucleases. in addition to the full-genome-length 6-kilobase pair linear dna, a 5.4-kilobase pair circular dna molecule, an incomplete linear dna molecule, and a 600-base pair molecule were detected. the 6-kilobase pair dna contained a 600-base pair direct terminal ... | 1980 | 6245239 |
| characterization of tumorigenic and metastatic properties of murine sarcoma virus-transformed nonproducer balb/3t3 cell lines. | 1980 | 6245299 | |
| in vitro production and titration assays of b-tropic retroviruses isolated from c57bl mouse tumors induced by radiation leukemia virus (radlv-rs): effect of dexamethasone. | the effect of dexamethasone (dxm) on the replication and titration assays of b-ecotropic radiation leukemia virus (radlv-rs) isolates was examined. the drug was shown to enhance in vitro virus release by otherwise low producer permissive cells. dxm also stimulated infectivity and focus formation in murine s+l--permissive cells and shortened the time-appearance of virus-induced foci. in contrast to foci observed in nontreated cultures, which are generally abortive and composed of only a few morph ... | 1980 | 6246028 |
| sarcoma growth factor (sgf): specific binding to epidermal growth factor (egf) membrane receptors. | cells transformed by murine sarcoma viruses (msv) produce and release into their tissue culture media several polypeptide growth stimulating factors. one of these has been partially purified using bio-gel p-60 column chromatography followed by deae-cellulose chromatography. this growth factor was assigned the name sarcoma growth factor (sgf), and is here shown to require the epidermal growth factor (egf) receptor in order to function as a growth factor. deae-cellulose chromatography yielded a pr ... | 1980 | 6246130 |