Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| protection of chickens after live and inactivated virus vaccination against challenge with nephropathogenic infectious bronchitis virus pa/wolgemuth/98. | protection provided by live and inactivated virus vaccination against challenge with the virulent nephropathogenic infectious bronchitis virus (nibv) strain pa/wolgemuth/98 was assessed. vaccinations with combinations of live attenuated strains massachusetts (mass) + connecticut (conn) or mass + arkansas (ark) were given by eyedrop to 2-wk-old specific-pathogen-free leghorn chickens. after live infectious bronchitis virus (ibv) vaccination, some chickens at 6 wk of age received an injection of e ... | 2002 | 12495055 |
| infectious disease survey of lesser prairie chickens in north texas. | lesser prairie chicken (tympanuchus pallidicinctus) abundance, like that of most grassland birds, has declined rangewide for decades. although habitat loss and degradation are likely ultimate causes for this decline, infectious agents, particularly microparasites, could be proximate contributors. no surveys of pathogenic bacteria or viruses have been published for this species. we surveyed 24 free-living lesser prairie chickens from hemphill county, texas (usa), for evidence of exposure to salmo ... | 2002 | 12528454 |
| epididymal lithiasis in roosters and efferent ductule and testicular damage. | epididymal stones have been reported in roosters in the usa and japan. the cause of this dysfunction, which is associated with low fertility, is not known. the hypothesis of the present study is that a potential cause is the aggressive selection of birds over many centuries based upon female egg laying traits, without concern for potential effects on the male. if this hypothesis is correct, one potential consequence would be the presence of epididymal stones only in domesticated fowl and this ob ... | 2002 | 12530920 |
| genetic diversity of avian infectious bronchitis virus california variants isolated between 1988 and 2001 based on the s1 subunit of the spike glycoprotein. | twenty-nine isolates of avian infectious bronchitis virus (ibv) recovered from commercial chicken flocks in california between 1988 and 2001 and identified as california variants by serotype and direct automated cycle sequencing of the ibv spike glycoprotein s1 subunit, were further characterized phylogenetically and by nucleotide sequence comparison. california variants were grouped according to production type of chicken, by comparison with public access sequence databases (ncbi genbank and em ... | 2003 | 12536299 |
| in vivo thymulin treatments enhance avian lung natural killer cell cytotoxicity in response to infectious bronchitis virus. | previous work has shown that in vitro thymulin treatments have the ability to enhance natural killer (nk) cell cytotoxicity. the purpose of the experiments presented here was to examine the in vivo effects of thymulin on avian nk cell activity in response to a viral infection. five and a half-week-old k-strain chickens infected with avian infectious bronchitis virus (ibv) served as the model for these experiments. daily thymulin injections began at varying time points prior to or post-infection. ... | 2003 | 12538040 |
| detection and estimation of avian infectious bronchitis virus antigen by a novel indirect liquid-phase blocking enzyme-linked immunosorbent assay using chicken and rabbit affinity purified immunoglobulins. | an indirect liquid-phase blocking (lpb) enzyme-linked immunosorbent assay (elisa) using chicken and rabbit affinity purified immunoglobulin g (igg) has been developed to detect and estimate avian infectious bronchitis virus (ibv) antigen concentration directly in infected allantoic fluid. the method is based on the principle of binding of specific igg to the test ibv antigen and the assay of unbound igg on an antigen-coated elisa plate. the immunoglobulins are chicken n-terminal s2 peplomeric pr ... | 2002 | 12593737 |
| serum levels of mannan-binding lectin in chickens prior to and during experimental infection with avian infectious bronchitis virus. | mannan-binding lectin (mbl) is a glycoprotein and a member of the c-type lectin super family, the collectin family, and the acute phase protein family. the mbl exerts its function by directly binding to microbial surfaces through its carbohydrate recognition domains, followed by direct opsonization or complement activation via mbl-associated serine proteases (masp)-1 and -2. thus, mbl plays a major role in the first-line innate defense against pathogens. we investigated the mbl concentrations in ... | 2003 | 12619800 |
| susceptibility of three genetic lines of chicks to infection with a nephropathogenic t strain of avian infectious bronchitis virus. | mortality rates were compared in three genetic lines of specific pathogen-free chicks inoculated with one of two doses of a nephropathogenic strain of avian infectious bronchitis (ib) virus. the mortality rates were influenced primarily by the chick strain, but also by age and dose of virus. chicks of the inbred s line were highly susceptible. after inoculation with a low dose of virus at 2 and 4 weeks of age, mortality was 90 and 45%, respectively. chicks of the hwl non-inbred line were also su ... | 2003 | 12634084 |
| memory t cells protect chicks from acute infectious bronchitis virus infection. | infectious bronchitis has remained one of the most difficult to control diseases in poultry since it was first described in 1931. previous studies demonstrated that primary cd8(+) t lymphocytes collected at 10 days post-infection (p.i.) are important in controlling acute infection. to further investigate the role of memory t cells in protection, t lymphocytes collected from b19/b19 chicken spleens at 2, 3, 4, and 6 weeks p.i. were transferred to six-day-old syngeneic chicks one day prior to chal ... | 2003 | 12642110 |
| detection of infectious bronchitis virus by multiplex polymerase chain reaction and sequence analysis. | a multiplex reverse transcription-polymerase chain reaction (rt-pcr) was developed to amplify the s1 and s2 genes of vaccine and recent taiwanese isolates of infectious bronchitis virus (ibv). dna fragments of 228 and 400 base pairs in length were amplified among ibv isolates in multiplex pcr, suggesting that there were no apparent deletions or insertions in these regions. no pcr products were amplified from unrelated avian viruses and negative controls. the results suggested that multiplex pcr ... | 2003 | 12668265 |
| avian infectious bronchitis: characterization of new isolates from italy. | the isolation of four new variants or serotypes of avian infectious bronchitis virus in italy is reported. the antigenic characteristics of these strains were investigated by cross-neutralization tests with the new isolates, fa 6881/97, az 27/98, az 20/97, and bs 216/01; two of the most common european serotypes, az 23/74 and cr 88121 (793b); and the classic massachusetts m41 serotype in association with a panel of 17 specific antisera. on the basis of the results obtained, the new isolates show ... | 2003 | 12713175 |
| in vitro and in ovo expression of chicken gamma interferon by a defective rna of avian coronavirus infectious bronchitis virus. | coronavirus defective rnas (d-rnas) have been used for site-directed mutagenesis of coronavirus genomes and for expression of heterologous genes. d-rna cd-61 derived from the avian coronavirus infectious bronchitis virus (ibv) was used as an rna vector for the expression of chicken gamma interferon (chifn-gamma). d-rnas expressing chifn-gamma were shown to be capable of rescue, replication, and packaging into virions in a helper virus-dependent system following electroporation of in vitro-derive ... | 2003 | 12719562 |
| a recombinant fowl adenovirus expressing the s1 gene of infectious bronchitis virus protects against challenge with infectious bronchitis virus. | the spike peplomer s1 subunit sequence from avian infectious bronchitis virus (ibv) vic s strain was expressed in a plasmid under the control of the fowl adenovirus (fav) major late promoter (mlp). two recombinants were constructed in fav serotype 8 (fav 8) by inserting the expression cassette between the snabi and xbai restriction enzyme sites (clone da3) or between the spei sites (clone ca6-20). expression of the s1 gene in the recombinants was confirmed by reverse transcription-polymerase cha ... | 2003 | 12798610 |
| in vitro compatibility of an anticoccidial vaccine with live infectious bronchitis and live newcastle disease vaccines. | 2003 | 12825705 | |
| emergence of a coronavirus infectious bronchitis virus mutant with a truncated 3b gene: functional characterization of the 3b protein in pathogenesis and replication. | the subgenomic rna 3 of ibv has been shown to be a tricistronic mrna, encoding three products in ibv-infected cells. to explore if the least expressed orf, orf 3b, which encodes a nonstructural protein, is evolutionarily conserved and functionally indispensable for viral propagation in cultured cells, the beaudette strain of ibv was propagated in chicken embryonated eggs for three passages and then adapted to a monkey kidney cell line, vero. the 3b gene of passage 3 in embryonated eggs and passa ... | 2003 | 12832199 |
| recombinant avian infectious bronchitis virus expressing a heterologous spike gene demonstrates that the spike protein is a determinant of cell tropism. | a recombinant infectious bronchitis virus (ibv), beaur-m41(s), was generated using our reverse genetics system (r. casais, v. thiel, s. g. siddell, d. cavanagh, and p. britton, j. virol. 75:12359-12369, 2001), in which the ectodomain region of the spike gene from ibv m41-ck replaced the corresponding region of the ibv beaudette genome. beaur-m41(s) acquired the same cell tropism phenotype as ibv m41-ck in four different cell types, demonstrating that the ibv spike glycoprotein is a determinant o ... | 2003 | 12885925 |
| expression of immunogenic s1 glycoprotein of infectious bronchitis virus in transgenic potatoes. | the expression of infectious bronchitis virus (ibv) s1 glycoprotein in potatoes and its immunogenicity in mice and chickens were investigated. potato plants were genetically transformed with a cdna construct encoding the ibv s1 glycoprotein with the agrobacterium system. genomic dna and mrna analyses of the transformed plantlets confirmed the integration of the foreign cdna into the potato genome, as well as its transcription. mice and chickens vaccinated with the expressed ibv s1 glycoprotein p ... | 2003 | 12885926 |
| protection of chickens from infectious bronchitis by in ovo and intramuscular vaccination with a dna vaccine expressing the s1 glycoprotein. | we have constructed a dna vaccine (pdkarks1-dpi) expressing the s1 glycoprotein (arkansas dpi) of infectious bronchitis virus (ibv) to examine protective immunity after in ovo and intramuscular dna immunization. birds receiving in ovo dna followed by live virus vaccination at 2 wk of age were 100% protected from clinical disease. birds receiving only live virus vaccine or only in ovo dna vaccination were < or = 80% protected. ibv was detected up to 10 days postchallenge in unvaccinated control g ... | 2003 | 12887187 |
| evaluation of the non-temperature-sensitive field clonal isolates of the mycoplasma synoviae vaccine strain ms-h. | the live attenuated temperature-sensitive (ts+) mycoplasma synovia (ms) strain, ms-h, is used as a vaccine in a number of countries to control virulent ms infection in commercial chicken flocks. nine out of 50 isolates made from flocks vaccinated with ms-h were found to have lost the ts+ phenotype of the original vaccine strain. in order to examine the influence of the ts- phenotype on virulence of the isolates, four of the ts- isolates, the ms-h vaccine, and the vaccine parent strain 86079/7ns ... | 2003 | 12887194 |
| the cytoplasmic tails of infectious bronchitis virus e and m proteins mediate their interaction. | virus-like particle (vlp) formation by the coronavirus e and m proteins suggests that interactions between these proteins play a critical role in coronavirus assembly. we studied interactions between the infectious bronchitis virus (ibv) e and m proteins using in vivo crosslinking and vlp assembly assays. we show that ibv e and m can be crosslinked to each other in ibv-infected and transfected cells, indicating that they interact. the cytoplasmic tails of both proteins are important for this int ... | 2003 | 12890618 |
| hematology, plasma biochemistry, and serosurvey for selected infectious agents in southern giant petrels from patagonia, argentina. | in conjunction with reproductive and feeding ecology studies on southern giant petrels (sgp, macronectes giganteus) blood samples were collected for baseline health evaluations. twenty-five adult sgp from a breeding colony in chubut, argentina, were sampled during two consecutive breeding seasons, 1999-2000 (n = 15) and 2000-01 (n = 10). values for hematology, plasma biochemistry, and minerals are described for 20 birds in apparent good physical condition. a serologic survey of exposure to selec ... | 2003 | 12910764 |
| typing of field isolates of infectious bronchitis virus based on the sequence of the hypervariable region in the s1 gene. | a universal primer set was developed that amplifies a region covering hypervariable region (hvr) 1 and hvr 2 in the s1 gene of the infectious bronchitis virus (ibv). the universality of this primer set was confirmed by testing the reference strains of different serotypes or variants of the ibv present in the united states. an approximately 450-bp region containing hvr 1 and hvr 2 of 7 untyped field isolates obtained in 1999 and 2000 was amplified. direct sequencing followed by phylogenetic analy ... | 2003 | 12918815 |
| electron microscope studies of four strains of infectious bronchitis virus. | 1952 | 12976644 | |
| transmission of the avian infectious bronchitis virus to the cynomolgus monkey. | 1954 | 13200090 | |
| stability of avian infectious bronchitis virus at 56 c. | 1956 | 13277330 | |
| propagation of infectious bronchitis virus in tissue culture. | 1956 | 13364746 | |
| symposium on immunization against infectious bronchitis virus. iii. infectious bronchitis control in chickens. | 1957 | 13416121 | |
| an electron microscope study of chorioallantoic membrane infected with the virus of avian infectious bronchitis. | 2005 | 13416637 | |
| symposium on immunization against infectious bronchitis virus. i. some basic properties of infectious bronchitis virus. | 1957 | 13444588 | |
| antigenic differences among isolates of avian infectious bronchitis virus. | 1958 | 13559632 | |
| influence of age and congenital immunity upon immunization with an avian infectious bronchitis vaccine. | 1958 | 13563894 | |
| differentiation of quail bronchitis virus and infectious bronchitis virus by heat stability. | 1960 | 13724630 | |
| hemagglutination by trypsin-modified infectious bronchitis virus. | 1959 | 13811911 | |
| a study of infectious bronchitis virus before and after resin treatment for separation from a cell-attachment-and-growth factor. | 1963 | 13932582 | |
| the structure of infectious bronchitis virus. | 1964 | 14194135 | |
| some studies of infectious bronchitis virus in cell culture. | 1965 | 14253989 | |
| direct and indirect complement-fixation tests for infectious bronchitis virus. | 1964 | 14266877 | |
| lack of correlation between infectivity, serologic response and challenge results in immunization with an avian infectious bronchitis vaccine. | 1965 | 14299027 | |
| ability of massachusetts-type infectious bronchitis virus to increase colibacillosis susceptibility in commercial broilers: a comparison between vaccine and virulent field virus. | the abilities of massachusetts-type vaccine virus and virulent infectious bronchitis (ib) field virus to increase colibacillosis susceptibility were compared. in four experiments, 29-day-old female commercial broilers housed in isolators, were infected intratracheally and oculonasally with ib vaccine strains (h120 and h52) or virulent ib field strains (d387 and m41) (4.8 or 6.8 log(10) median embryo infective dose, per broiler). five days later, escherichia coli 506 strain was given intratrachea ... | 2003 | 14522702 |
| evaluation of a nucleoprotein-based enzyme-linked immunosorbent assay for the detection of antibodies against infectious bronchitis virus. | as an immunogen of the coronavirus, the nucleoprotein (n) is a potential antigen for the serological monitoring of infectious bronchitis virus (ibv). in this report, recombinant n protein from the beaudette strain of ibv was produced and purified from escherichia coli as well as sf9 (insect) cells, and used for the coating of enzyme-linked immunosorbent assay (elisa) plates. the n protein produced in sf9 cells was phosphorylated whereas n protein from e. coli was not. our data indicated that n p ... | 2003 | 14522708 |
| establishment of persistent avian infectious bronchitis virus infection in antibody-free and antibody-positive chickens. | avian infectious bronchitis virus (ibv) causes a highly contagious and economically significant disease in chickens. establishment of a carrier state in ibv infection and the potential for the persistent virus to undergo mutations and recombination in chicken tissues have important consequences for disease management. nevertheless, whether chickens can maintain persistent ibv infection in the absence of reinfection from exogenous sources or the presence of antibody in the host can modulate virus ... | 2003 | 14562886 |
| the effects of avian leukosis virus subgroup j on broiler chicken performance and response to vaccination. | the effects of avian leukosis virus subgroup j (alv-j) infection on meat-type chickens reared in a simulated commercial setting were evaluated. each of three alv-j isolates was evaluated with both simulated horizontal transmission (sht) and simulated vertical transmission (svt). mortality, morbidity, disease condemnations, and feed conversions were increased and body weights at processing were decreased in alv-j infected birds as compared to sham inoculated hatch mates. the adverse effects of al ... | 2003 | 14562889 |
| attenuation, safety, and efficacy of an infectious bronchitis virus ga98 serotype vaccine. | in 1998, novel strains of infectious bronchitis virus (ibv) were identified in chickens from the southeastern united states and classified as a new serotype designated georgia 98 (ga98). because of the widespread nature of the ga98 virus in the southeastern united states and the lack of adequate protection with the de072 vaccine, we developed a specific vaccine for the ga98 serotype. the ga98/0470/98 isolate of ibv was passaged in embryonating chicken eggs 70 times, and attenuation of the virus ... | 2003 | 14562890 |
| detection of infectious bronchitis virus by real-time reverse transcriptase-polymerase chain reaction and identification of a quasispecies in the beaudette strain. | in this report, we describe a real-time reverse transcriptase-polymerase chain reaction (rrt-pcr) diagnostic test for infectious bronchitis virus (ibv) with the use of fluorescence resonance energy transfer (fret) technology. two primers that amplify a 383-base pair product between nucleotide positions 703 and 1086 relative to the start codon for the s1 gene of the massachusetts 41 virus were designed and used to amplify the beaudette, massachusetts 41, florida 18288, connecticut, iowa 97, arkan ... | 2003 | 14562902 |
| characterization of infectious bronchitis virus isolates by slot blot hybridization. | we used slot blot hybridization of the hypervariable regions of the s1 subunit of spike peplomer gene to identify and characterize infectious bronchitis virus (ibv) strains. template dna was created from six reference strain ibvs of different serotypes and immobilized on a nitrocellulose membrane. we synthesized digoxigenin-labeled probes from reference and unknown field viruses and hybridized them to template dna. all reference strains could be distinguished and isolates identified by serotype ... | 2003 | 14562903 |
| evaluation of pathogenic potential of avian influenza virus serotype h9n2 in chickens. | recently seven isolates of avian influenza virus (aiv) serotype h9n2 recovered from an outbreak of ai were analyzed on the basis of their biological and molecular characteristics. all the isolates belonged to the low-pathogenicity group of aiv. to further evaluate their pathogenic potential in association with other organisms, an isolate was inoculated experimentally in chickens using different routes and subsequently challenged with infectious bronchitis virus, ornithobacterium rhinotracheale o ... | 2003 | 14575070 |
| avian influenza (h9n2) outbreak in iran. | an epidemic of avian influenza (ai) (h9n2) occurred in broiler chicken farms in iran during 1998-01. mortality between 20% and 60% was commonly observed on the affected farms. mixed infections of the influenza virus with other respiratory pathogens, particularly infectious bronchitis virus and mycoplasma gallisepticum, were thought to be responsible for such high mortality, which resulted in great economic losses. clinical signs included swelling of the periorbital tissues and sinuses, typical r ... | 2003 | 14575072 |
| hematology, plasma chemistry, serology, and chlamydophila status of the waved albatross (phoebastria irrorata) on the galapagos islands. | venipuncture was performed on 50 adult, free-ranging waved albatrosses (phoebastria irrorata) on española, galapagos islands, ecuador, to establish hematologic and plasma biochemistry reference ranges and to determine the prevalence of exposure to important domestic avian pathogens. weights and plasma creatine phosphokinase activities differed significantly between males and females. serum was tested for evidence of exposure to avian influenza, avian paramyxoviruses 1, 2, and 3, avian cholera, a ... | 2003 | 14582791 |
| [transgenic potato containing immunogenic gene of avian coronavirus and its immunogenicity in mice]. | to check the feasibility of expression of the immunogenic gene of avian coronavirus infectious bronchitis virus (ibv) in plants, the transformation of s1 gene of ibv into potato and the immunogenicity of its expression product was studied. the s1 gene of ibv-zj971 strain was inserted into plasmid pbi121 under the control of 35 s promoter. agrobacterium fumefaciens eha105 with the recombinant vector pbi121 was obtained by tri-parental mating method. so, an efficient potato transformation system m ... | 2003 | 14614539 |
| a reverse transcriptase-polymerase chain reaction assay for the diagnosis of turkey coronavirus infection. | this study reports on the development of a reverse transcriptase-polymerase chain reaction (rt-pcr) for the specific detection of turkey coronavirus (tcov). of the several sets of primers tested, 1 set of primers derived from the p gene and 2 sets derived from the n gene of tcov could amplify the tcov genome in the infected samples. the rt-pcr was sensitive and specific for tcov and did not amplify other avian rna and dna viruses tested except the infectious bronchitis virus (ibv). to overcome t ... | 2003 | 14667027 |
| severe acute respiratory syndrome vaccine development: experiences of vaccination against avian infectious bronchitis coronavirus. | vaccines against infectious bronchitis of chickens (gallus gallus domesticus) have arguably been the most successful, and certainly the most widely used, of vaccines for diseases caused by coronaviruses, the others being against bovine, canine, feline and porcine coronaviruses. infectious bronchitis virus (ibv), together with the genetically related coronaviruses of turkey (meleagris gallopovo) and ring-necked pheasant (phasianus colchicus), is a group 3 coronavirus, severe acute respiratory syn ... | 2003 | 14676007 |
| a recombination event, induced in ovo, between a low passage infectious bronchitis virus field isolate and a highly embryo adaptedvaccine strain. | the possibility of genomic recombination among different strains of infectious bronchitis virus (ibv) was examined in eve by coinfecting specific pathogen free embryonating chicken eggs with commonly used, embryo-adapted vaccine strains of ibv (arkansas, massachusetts, and connecticut), and a delaware-072-like field virus isolated from a layer farm in minnesota. recombination was observed between th e massachusetts and the delaware-072-like strains of the virus. the recombination event was asses ... | 2003 | 14708973 |
| evaluation of the protection conferred by commercial vaccines against the california 99 isolate of infectious bronchitis virus. | an infectious bronchitis virus (ibv) was isolated from commercial broilers from the state of california exhibiting respiratory distress, inflamed tracheas, airsaculitis, and edematous lungs. after reverse transcriptase-polymerase chain reaction (rt-pcr), the california isolate exhibited an identical restriction fragment length polymorphism (rflp) pattern to some isolates obtained from california, known as california 99 isolates. commercial mass-conn and mass-ark vaccines were used to vaccinate c ... | 2003 | 14708975 |
| studies on the diagnosis of newcastle disease and infectious bronchitis. iv. the use of the serum neutralization test in the diagnosis of infectious bronchitis. | 1951 | 14812846 | |
| infectious bronchitis and newcastle disease. | 1951 | 14821863 | |
| newcastle disease and infectious bronchitis neutralizing antibody indexes of normal chicken serum. | 1951 | 14829765 | |
| infectious bronchitis. | 1951 | 14829767 | |
| the persistence of infectious bronchitis virus in eggs and tracheal exudates of infected chickens. | 1951 | 14849148 | |
| interference between influenza virus and infectious bronchitis virus of chickens. | 1952 | 14938312 | |
| presence of an encephalomyocarditis virus internal ribosome entry site sequence in avian infectious bronchitis virus defective rnas abolishes rescue by helper virus. | avian infectious bronchitis virus (ibv) defective rnas (d-rnas) have been used for the expression of heterologous genes in a helper-virus-dependent expression system. the heterologous genes were expressed under the control of an ibv transcription-associated sequence (tas) derived from gene 5 of ibv beaudette. however, coronavirus d-rna expression vectors display an inherent instability following serial passage with helper virus, resulting in the eventual loss of the heterologous genes. the use o ... | 2004 | 14990691 |
| s1 glycoprotein gene analysis of infectious bronchitis viruses isolated in korea. | fifteen isolates of infectious bronchitis virus (ibv) were obtained from the kidney, trachea, and cecal tonsil of ib suspected chickens between 2001 and 2002 years in korea. the s1 glycoprotein gene of ibv isolates were amplified by reverse transcriptase - polymerase chain reaction (rt-pcr) and analyzed by restriction fragment length polymorphism (rflp) analysis. fifteen korean ibv isolates were classified into 4 groups by their rflp patterns using restriction enzymes, haeiii, bstyi, and xcmi. t ... | 2004 | 14991438 |
| comparisons of envelope through 5b sequences of infectious bronchitis coronaviruses indicates recombination occurs in the envelope and membrane genes. | a 1.78 kb sequence, including the e, m, 5a and 5b genes, and the intergenic region between the m and 5a genes, of six us strains of infectious bronchitis (corona)virus (ibv) were sequenced and compared to the published sequences for two additional strains. the overall identities as determined through pairwise analyses of nucleotide sequences of the entire 1.78kb region ranged from 90 to 99%, with the 5b open reading frame (orf) having the greatest identity (94-99%) while the identities of the e, ... | 2004 | 15019237 |
| host-dependent type 1 cytokine responses driven by inactivated viruses may fail to default in the absence of il-12 or ifn-alpha/beta. | replicating viruses generally induce type 1 immune responses, with high interferon (ifn)-gamma levels and antibodies of the igg2a isotype. in the present study we demonstrate the intrinsic ability of non-replicating virions to induce comparable immune responses in the notable absence of any adjuvant. injection of inactivated pseudorabies virus, an alphaherpesvirus, by various routes into mice resulted in the generation of t helper (th) 1 type immune response. co-delivery of inactivated pseudorab ... | 2004 | 15039522 |
| history of biological control of poultry diseases in the usa. | 2004 | 15077792 | |
| pathology and ultrastructure of an intranuclear bacilliform virus (ibv) infecting brown shrimp crangon crangon (decapoda: crangonidae). | the brown shrimp crangon crangon supports an important fishery in europe (over 25000 t, valued at 80 million euros in 2000). through the course of histopathological screening of crustaceans from the clyde estuary, western scotland, for the biological effect of contaminants, we have discovered a highly prevalent (up to 100%) non-occluded intranuclear bacilliform virus (ibv) infection in the hepatopancreatic tubule epithelia and midgut epithelia of wild c. crangon. this is the first report of an i ... | 2004 | 15109130 |
| intracellular targeting signals contribute to localization of coronavirus spike proteins near the virus assembly site. | coronavirus budding at the endoplasmic reticulum-golgi intermediate compartment (ergic) requires accumulation of the viral envelope proteins at this point in the secretory pathway. here we demonstrate that the spike (s) protein from the group 3 coronavirus infectious bronchitis virus (ibv) contains a canonical dilysine endoplasmic reticulum retrieval signal (-kkxx-cooh) in its cytoplasmic tail. this signal can retain a chimeric reporter protein in the ergic and when mutated allows transport of t ... | 2004 | 15140989 |
| detection of three avian respiratory viruses by single-tube multiplex reverse transcription-polymerase chain reaction assay. | acute respiratory tract infections are leading causes of morbidity in poultry farms throughout the world. avian pneumovirus (apv), avian influenza virus (aiv), and newcastle disease virus (ndv) have been recognized as the most important pathogens of both chicken and turkeys. single-virus reverse transcription-polymerase chain reaction (srt-pcr) assays are used extensively to detect these viruses in clinical samples. this study reports the development and evaluation of a single-tube multiplex rt- ... | 2004 | 15152843 |
| a survey of the prevalence of infectious bronchitis virus type 4/91 in iran. | to evaluate the prevalence of infectious bronchitis virus (ibv) type 4/91 in iran, tracheal swabs from 77 broiler flocks in 16 provinces were collected at the slaughterhouse. swabs were subjected to rna extraction and tested by rt-pcr, followed by a type-specific nested pcr. the viral rna was detected in 33 samples (42.8%) from different provinces. the results indicate a relatively high prevalence of ibv type 4/91 in iran and necessitate revising the vaccination programme against this disease. | 2004 | 15168747 |
| comparison of four regions in the replicase gene of heterologous infectious bronchitis virus strains. | infectious bronchitis virus (ibv) produces six subgenomic (sg) mrnas, each containing a 64 nucleotide (nt) leader sequence, derived from the 5' end of the genome by a discontinuous process. several putative functional domains such as a papain-like proteinase (pl(pro)), main protease (m(pro)), rna-dependent rna polymerase (rdrp), and rna helicase encoded by the replicase gene are important for virus replication. we have sequenced four regions of the replicase genes corresponding to the 5'-termina ... | 2004 | 15183070 |
| a serological survey for pathogens in old fancy chicken breeds in central and eastern part of the netherlands. | to get an impression of the presence of pathogens in multi-aged flocks of old fancy chicken breeds in the netherlands, plasma samples originating from 24 flocks were examined for antibodies against 17 chicken pathogens. these flocks were housed mainly in the centre and east of the netherlands, regions with a high poultry density. the owners of the tested flocks showed their chicken at national and international poultry exhibitions. antibodies against avian influenza, egg drop syndrome '76 virus, ... | 2004 | 15185615 |
| complete nucleotide sequences of s1 and n genes of infectious bronchitis virus isolated in japan and taiwan. | the complete nucleotide sequences of the s1 and n genes of three japanese and one taiwanese field strains of ibv are reported. these japanese strains were found to have s1 sequences most similar to those of australian strains and n sequences most similar to those of north american strains. this result suggested that these japanese strains might all be recombinant viruses derived from recombination of australia- and north america-related viruses. moreover, the s1 proteins of all these japanese an ... | 2004 | 15187369 |
| generation of the transgenic potato expressing full-length spike protein of infectious bronchitis virus. | to seek a new delivery system of vaccine for infectious bronchitis virus (ibv), transgenic potato expressing full-length spike (s) protein of ibv was produced and its immunogenicity in chickens was investigated. one to three copies of s gene of ibv were randomly and stably inserted into potato (solanum tuberrosum cv. dongnong 303) genome by agrobacterium tumefaciens-mediated transformation. transcription and translation of s gene for ibv were confirmed by northern blot and western blot analyses ... | 2004 | 15219399 |
| rapid serological profiling by an immunocomb-based dot-enzyme-linked immunosorbent test for three major poultry diseases. | an immunocomb-based dot-elisa, employing specially designed apparatus, was used to measure the antibody status for the three major poultry diseases--newcastle disease, infectious bursal disease and infectious bronchitis--in single test sera. positive samples could be classified into strong, moderate and weak positives by comparison with the colour reaction given by known strong and weak positive serum controls. the simultaneous dot-immunobinding assay gave reproducible results and allowed consid ... | 2004 | 15222738 |
| significance of interactions between escherichia coli and respiratory pathogens in layer hen flocks suffering from colibacillosis-associated mortality. | this study aimed to examine the significance of interactions between escherichia coli and various respiratory pathogens during outbreaks of colibacillosis-associated mortality in layer hen flocks under field conditions. for this purpose, a case-control study involving 20 control flocks with baseline mortality and 20 flocks with increased mortality due to e. coli septicaemia and polyserositis, was conducted. in each colibacillosis flock, blood samples were taken from 20 hens at the onset of clini ... | 2004 | 15223556 |
| microwave or autoclave treatments destroy the infectivity of infectious bronchitis virus and avian pneumovirus but allow detection by reverse transcriptase-polymerase chain reaction. | a method is described for enabling safe transit of denatured virus samples for polymerase chain reaction (pcr) identification without the risk of unwanted viable viruses. cotton swabs dipped in avian infectious bronchitis virus (ibv) or avian pneumovirus (apv) were allowed to dry. newcastle disease virus and avian influenza viruses were used as controls. autoclaving and microwave treatment for as little as 20 sec destroyed the infectivity of all four viruses. however, both ibv and apv could be d ... | 2004 | 15223557 |
| egg:embryo weight ratio as an indicator of dwarfism induced by infectious bronchitis virus. | a simple objective method to quantify embryo dwarfism induced by infectious bronchitis virus in embryonated chicken eggs has been used to determine endpoints in virus titration and neutralization assays. the eggs and the respective embryos were weighed and embryo:egg weight (ee) ratios were calculated. the ee ratios were compared with the uninoculated control eggs and endpoints could be calculated objectively. ee indices were also calculated by dividing the ee ratios of inoculated embryonated ch ... | 2004 | 15223558 |
| a new genotype of nephropathogenic infectious bronchitis virus circulating in vaccinated and non-vaccinated flocks in china. | five strains of infectious bronchitis virus (ibv) were isolated from five layer flocks that had nephropathogenic infection in four provinces in china. among them, three of the five flocks had been vaccinated against infectious bronchitis. virulence studies indicated that the five chinese ibv isolates caused 10 to 30% mortality in 15-day-old specific pathogen free chickens and gross lesions were mainly confined to the kidneys in all of the dead chickens. two oligonucleotide pairs, s1uni2 and s1ol ... | 2004 | 15223561 |
| characterization of an avian infectious bronchitis virus isolated in china from chickens with nephritis. | one ibv isolate, sc021202, was isolated from the kidneys of the infected young chickens by inoculating embryonated eggs, and its morphology, physiochemical and haemagglutonating properties were detected. virulence of the isolate sc021202 was determined with specific pathogen-free (spf) chicken inoculation. nucleotide acid sequence of s1 gene of the isolate sc021202 was further sequenced and analysed. the physiochemical and morphological properties of the isolate sc021202 were in accordance to th ... | 2004 | 15228547 |
| construction, characterization, and evaluation of the vaccine potential of three genetically defined mutants of avian pathogenic escherichia coli. | the delta gale, delta pura, and delta aroa derivatives of avian septicemic escherichia coli ec99 strain (o78 serogroup) were constructed with a suicide vector containing the pir-dependent r6k replicon and the sacb gene of bacillus subtilis. the resultant isogenic mutants were stable and lacked approximately 45%, 36%, and 52% of the genes for gale, pura, and aroa, respectively. the delta pura and delta aroa mutants did not grow on minimal medium, whereas the delta gale mutant grew on minimal medi ... | 2004 | 15283416 |
| phylogenetic analysis of avian infectious bronchitis virus strains isolated in japan. | to define the origin and evolution of recent avian infectious bronchitis virus (ibv) in japan, a genetic analysis was performed. by phylogenetic analysis based on the s1 gene including the sequence of the hypervariable regions, ibv isolates in japan were classified into five genetic groups, which included two already-known groups (mass and gray). among them, three major genetic groups were associated with the recent outbreaks of ib in japan. one group is indigenous to japan and could not be plac ... | 2004 | 15290359 |
| nephropathogenesis of chickens experimentally infected with various strains of infectious bronchitis virus. | four-day-old specific-pathogen-free chickens were inoculated by eyedrop with four different strains (gray, jmk, cv56b, and wolgemuth) of infectious bronchitis virus (ibv). birds were monitored clinically and euthanatized at 1, 4, 7, and 14 days postinfection and tissues were collected for virus isolation, histopathologic examination, in situ hybridization (ish), and immunohistochemistry (ihc). clinical disease was severe in chickens infected with wolgemuth, but no overt disease was observed with ... | 2004 | 15297756 |
| a single amino acid mutation in the spike protein of coronavirus infectious bronchitis virus hampers its maturation and incorporation into virions at the nonpermissive temperature. | the spike (s) glycoprotein of coronavirus is responsible for receptor binding and membrane fusion. a number of variants with deletions and mutations in the s protein have been isolated from naturally and persistently infected animals and tissue cultures. here, we report the emergence and isolation of two temperature sensitive (ts) mutants and a revertant in the process of cold-adaptation of coronavirus infectious bronchitis virus (ibv) to a monkey kidney cell line. the complete sequences of wild ... | 2004 | 15302214 |
| pathology of the chicken embryo infected with infectious bronchitis virus. | 1950 | 15425747 | |
| specific antibody secreting cells from chickens can be detected by three days and memory b cells by three weeks post-infection with the avian respiratory coronavirus. | infectious bronchitis virus (ibv), the first coronavirus described, has been a continuing problem in poultry for more than 70 years. ibv, causing a highly contagious respiratory disease in chickens, resembles the recently described severe acute respiratory syndrome virus in pathogenesis and genome organization. while previous studies demonstrated that effector and memory cd8(+) t lymphocytes are critical in controlling acute ibv infection and disease in chickens, here chicken anti-ibv antibody ( ... | 2005 | 15450755 |
| methods to enhance the intensity of intranuclear bacilliform virus infection in cherax quadricarinatus. | many studies have examined the morphology, pathology and epizootiology of the intranuclear bacilliform virus (ibv) of cherax quadricarinatus, but little research has been conducted to acquire specific knowledge of the virus. this is partly due to difficulties in detecting the virus and in obtaining sufficient material for viral isolation and purification. as quantified by light microscopy, we significantly (p < 0.01) enhanced ibv intensities from 10.56 to 16.67% in c. quadricarinatus by using sa ... | 2004 | 15460862 |
| interaction between genomes of infectious bronchitis and newcastle disease viruses studied by reverse transcription-polymerase chain reaction. | reverse transcription-polymerase chain reaction (rt-pcr) with specific primers for the s1 gene of ibv and for the fusion protein cleavage site of ndv was used for detection of infectious bronchitis virus (ibv, the family coronaviridae) and newcastle disease virus (ndv) genomes. the sensitivity of ibv and ndv rt-pcr was 10(3.7) and 10(3.0) eid50, respectively. although a multiplex rt-pcr could detect and differentiate ndv and ibv genomes present in the same sample, there was a slight inhibition o ... | 2004 | 15462288 |
| testing the hypothesis of a recombinant origin of the sars-associated coronavirus. | the origin of severe acute respiratory syndrome-associated corona-virus (sars-cov) is still a matter of speculation, although more than one year has passed since the onset of the sars outbreak. in this study, we implemented a 3-step strategy to test the intriguing hypothesis that sars-cov might have been derived from a recombinant virus. first, we blasted the whole sars-cov genome against a virus database to search viruses of interest. second, we employed 7 recombination detection techniques wel ... | 2005 | 15480857 |
| complete sequences of 3' end coding region for structural protein genes of turkey coronavirus. | overlapping fragments of genomic rna spanning 6963 nucleotides from 5' end of spike (s) protein gene to 3' end of nucleocapsid (n) protein gene of turkey coronavirus (tcov) were amplified by reverse-transcription-polymerase chain reaction (rt-pcr). the primers were derived from the corresponding sequences of infectious bronchitis virus (ibv). the pcr products were cloned and sequenced and their nucleic acid structure and similarity to published sequences of other coronaviruses were analyzed. seq ... | 2004 | 15522448 |
| s1 and n gene analysis of avian infectious bronchitis viruses in taiwan. | the disease caused by infectious bronchitis virus (ibv) produces great economic for the poultry industry. the purpose of this study is to investigate the molecular epidemiology of ibv in taiwan. an old ibv strain isolated in 1964 and another 31 strains isolated from 1991 to 2003 were selected for n-terminal s1 gene analysis. based on their phylogenetic tree, 13 strains were selected for sequencing the entire s1 and partial nucleocapsid (n) genes. the results indicated that taiwanese ibv strains ... | 2004 | 15529980 |
| identification of a novel nephropathogenic infectious bronchitis virus in israel. | a novel infectious bronchitis variant, designated as is/885/00, associated with nephritis, was isolated from outbreaks in 23 broiler farms in israel. the virus was first identified by reverse transcriptase-polymerase chain reaction and showed a distinct restriction fragment length polymorphism pattern from previously described israeli isolates. sequence analysis of the s1 gene and the deduced amino acid sequence revealed 97.2% protein similarity to genotype is/ 720/99 and 71.6% similarity to the ... | 2004 | 15529987 |
| recombinant infectious bronchitis coronavirus beaudette with the spike protein gene of the pathogenic m41 strain remains attenuated but induces protective immunity. | we have replaced the ectodomain of the spike (s) protein of the beaudette strain (beau-r; apathogenic for gallus domesticus chickens) of avian infectious bronchitis coronavirus (ibv) with that from the pathogenic m41 strain to produce recombinant ibv beaur-m41(s). we have previously shown that this changed the tropism of the virus in vitro (r. casais, b. dove, d. cavanagh, and p. britton, j. virol. 77:9084-9089, 2003). herein we have assessed the pathogenicity and immunogenicity of beaur-m41(s). ... | 2004 | 15564488 |
| coronavirus genome structure and replication. | in addition to the sars coronavirus (treated separately elsewhere in this volume), the complete genome sequences of six species in the coronavirus genus of the coronavirus family [avian infectious bronchitis virus-beaudette strain (ibv-beaudette), bovine coronavirus-ent strain (bcov-ent), human coronavirus-229e strain (hcov-229e), murine hepatitis virus-a59 strain (mhv-a59), porcine transmissible gastroenteritis-purdue 115 strain (tgev-purdue 115), and porcine epidemic diarrhea virus-cv777 strai ... | 2005 | 15609507 |
| reverse genetics of coronaviruses using vaccinia virus vectors. | in this article, we describe the reverse genetic system that is based on the use of vaccinia virus cloning vectors. this system represents a generic approach to coronavirus reverse genetics and was first described for the generation of recombinant human coronavirus 229e representing a group i coronavirus. subsequently, the same approach has been used to generate recombinant avian infectious bronchitis coronavirus and, recently, recombinant mouse hepatitis virus, representing group iii and group ... | 2005 | 15609513 |
| mass spectroscopic characterization of the coronavirus infectious bronchitis virus nucleoprotein and elucidation of the role of phosphorylation in rna binding by using surface plasmon resonance. | phosphorylation of the coronavirus nucleoprotein (n protein) has been predicted to play a role in rna binding. to investigate this hypothesis, we examined the kinetics of rna binding between nonphosphorylated and phosphorylated infectious bronchitis virus n protein with nonviral and viral rna by surface plasmon resonance (biacore). mass spectroscopic analysis of n protein identified phosphorylation sites that were proximal to rna binding domains. kinetic analysis, by surface plasmon resonance, i ... | 2005 | 15613344 |
| generation of a recombinant avian coronavirus infectious bronchitis virus using transient dominant selection. | a reverse genetics system for the avian coronavirus infectious bronchitis virus (ibv) has been described in which a full-length cdna, corresponding to the ibv (beaudette-ck) genome, was inserted into the vaccinia virus genome following in vitro assembly of three contiguous cdnas [casais, r., thiel, v., siddell, s.g., cavanagh, d., britton, p., 2001. reverse genetics system for the avian coronavirus infectious bronchitis virus. j. virol. 75, 12359-12369]. the method has subsequently been used to ... | 2005 | 15620403 |
| structure-activity relations of water-in-oil vaccine formulations and induced antigen-specific antibody responses. | water-in-oil (w/o) emulsions are known as most effective adjuvants to generate high and durable antibody responses to vaccine antigens following a single immunization. however, their structural requirements remain poorly understood. here we addressed the significance of certain pharmaceutical characteristics including water/oil ratios--ranging from 60/40 to 30/70 (w/w(%))--droplet size and type of oil, i.e. non-metabolizable (mineral oil) versus metabolizable (miglyol 840). stability of emulsion ... | 2005 | 15620479 |
| in vitro assembled, recombinant infectious bronchitis viruses demonstrate that the 5a open reading frame is not essential for replication. | molecular clones of infectious bronchitis virus (ibv), derived from the vero cell adapted beaudette strain, were constructed, using an in vitro assembly method. in vitro transcribed rna from a cdna template that had been constructed from seven cdna fragments, encompassing the entire genome of ibv, was electroporated into bhk-21 cells. the cells were overlaid onto the susceptible vero cells and viable virus was recovered from the molecular clone. the molecularly cloned ibv (mibv) demonstrated gro ... | 2005 | 15661153 |
| rapid differentiation of avian infectious bronchitis virus isolates by sample to residual ratio quantitation using real-time reverse transcriptase-polymerase chain reaction. | a rapid diagnostic assay for differentiating avian infectious bronchitis virus (ibv) isolates was developed. the basis of the assay is the cleavage of target rna by rnase h mediated by sequence-specific chimeric oligonucleotides followed by sample to residual ratio quantitation (srrq) using rrt-pcr. four serotype-specific chimeric oligonucleotides were designed, one each for the massachusetts, connecticut, arkansas, and delaware/georgia 98 serotypes, and tested for their ability to mediate speci ... | 2005 | 15664067 |
| avian infectious bronchitis virus: a possible cause of reduced fertility in the rooster. | the formation of epididymal stones in the rooster epididymis is a widespread problem that has detrimental effects on sperm production and fertility. the cause of epididymal stones is unknown, but an infectious agent, the avian infectious bronchitis virus (aibv), has been implicated. the goal of this study was to determine if administering the live attenuated aibv vaccine to male chicks increases the incidence of stones in the epididymal region of the adult rooster. specific pathogen free (spf) l ... | 2004 | 15666874 |
| oie white spot syndrome virus pcr gives false-positive results in cherax quadricarinatus. | white spot syndrome virus (wssv) is an intranuclear bacilliform virus (ibv) that is a serious, notifiable crustacean pathogen. the office international des epizooties (oie) pcr protocol for wssv uses primer sets initially developed by lo et al. (1996). it yields a first-step pcr amplicon of 1441 bp and a nested pcr amplicon of 941 bp. an amplicon (941 bp) purported to specifically detect wssv was obtained when using template dna extracted from cherax quadricarinatus in a wssv pcr detection proto ... | 2004 | 15672884 |