Publications

mutations abolishing the endonuclease activity of bacteriophage lambda terminase lie in two distinct regions of the a gene, one of which may encode a "leucine zipper" dna-binding domain.	bacteriophage lambda terminase is a multifunctional enzyme composed of two subunits which are the products of the phage-encoded nu1 and a genes. the enzyme catalyzes the endonucleolytic cleavage of lambda dna at a site known as cosn and mediates packaging of the phage dna into empty heads. this work describes the characterization of mutations within the a gene which lead to the loss of terminase endonuclease activity without affecting the ability of the enzyme to package monomeric mature (cut) l ...	1992	1534952
the phage lambda gene q transcription antiterminator binds dna in the late gene promoter as it modifies rna polymerase.	the bacteriophage lambda gene q transcription antiterminator modifies rna polymerase during an extended pause in elongation at nt +16 and +17 of the phage late gene promoter transcript. we show here that q binds a specific dna site between the -10 and -35 elements of the promoter as it interacts with the enzyme. we show that the pause must reflect a specialized elongation structure that is receptive to modification by q, because q does not bind to rna polymerase stopped artificially after transc ...	1992	1535556
a procedure for the construction of linear restriction fragment maps of a 50- to 100-kb dna.	a simple and effective procedure for the construction of linear restriction fragment maps was developed. using a two-enzyme digestion, two-dimensional (2-d) electrophoresis procedure, all the restriction fragments in a 50- to 100-kb dna can be individually resolved and displayed on a 2-d plane. this 2-d gel pattern, with appropriate markers, provides a fixed set of x, y coordinates for each fragment obtained from the single and double digestion as well as the relationship between the two steps. ...	1992	1535761
in vitro amplification of dna fragments greater than 10 kb.	the synthesis of a 10.9-kb dna fragment from a bacteriophage lambda template was used in the search for conditions to extend the range for the polymerase chain reaction (pcr). using the same primer sequences and conditions (denaturation at 94 degrees c, 1 min; annealing at 57 degrees c, 1 min; polymerization at 70 degrees c, 20 to 30 min) as published by w. rychlik, w. j. spencer, and r. e. rhoads [(1990) nucleic acids res. 18, 6409-6412], unsatisfactory results were obtained with amplitaq and n ...	1992	1535762
the mink gene for the lambda light immunoglobulin chain: characterization of cdna and chromosomal localization.	a cdna library from mink spleen was constructed by use of the phage lambda gt11. the library was screened using polyvalent serum raised against the mink immunoglobulin lambda chain. as a result, several clones expressing mink immunoglobulin lambda light chains were identified. sequencing of one of the clones with an 803 bp insert was performed. the insert comprised nearly the entire coding region for the mature lambda light immunoglobulin gene with the exception of the leader polypeptide and sev ...	1992	1543908
requirement for e. coli nusg protein in factor-dependent transcription termination.	the 21 kd nusg protein is essential for e. coli viability. cells depleted for nusg were defective for factor-dependent transcription termination. rho-induced polarity in the gal operon and the rho-dependent lambda tr1 and lambda tl1 terminators were suppressed in nusg-deficient cells. nusg depletion inactivated the phage hk022 nun termination factor. in contrast, the factor-independent lambda tl terminator was fully active in nusg-depleted cells and could be suppressed by phage lambda n function ...	1992	1547498
t41 mutation in lac repressor is tyr282----asp.	a monomeric mutant of the lac repressor protein, designated t41, produced originally by the in vivo mutt mutagenesis exhibited behavior similar to mutants identified as tyr282----ser or tyr282----gln [schmitz et al., j. biol. chem. 251 (1976) 3359-3366]. the t41 gene, encoded within a phage lambda prophage in the escherichia coli genome, was amplified by polymerase chain reaction and sequenced. the only mutation found in the nucleotide sequence corresponded to position 282 in the protein, and th ...	1992	1547952
the human immunoglobulin kappa locus. characterization of the duplicated a regions.	the central regions of the kappa locus, the so-called a regions, have been fully characterized on cosmid and phage lambda clones. the regions, which are parts of the c kappa-proximal and -distal copies of the locus and are, therefore, called ap and ad regions, comprise about 140 kb each and contain together 30 v kappa genes and pseudogenes. the a regions have been linked on their 5' sides to the o regions and on their 3' sides to the l regions. chromosomal walking has eliminated a previous gap i ...	1992	1551402
simultaneous gain and loss of functions caused by a single amino acid substitution in the beta subunit of escherichia coli rna polymerase: suppression of nusa and rho mutations and conditional lethality.	transcript elongation and termination in escherichia coli is modulated, in part, by the nusa gene product, an acidic protein that interacts not only with rna polymerase itself but also with ancillary factors, namely the host termination protein rho and phage lambda antitermination protein, n. the e. coli nusa1 mutant fails to support lambda development due to a specific defect in n-mediated antitermination. certain rifampicin-resistant (rifr) variants of the nusa1 host support lambda growth. we ...	1992	1551568
structure of the human ferrochelatase gene. exon/intron gene organization and location of the gene to chromosome 18.	we have determined the structure of the human ferrochelatase gene after isolation and characterization of lambda phage clones mapping discrete regions of the cdna. this gene was assigned to human chromosome 18 at region q21.3, by fluorescent in situ hybridization. the gene contains a total of 11 exons and has a minimum size of about 45 kb. the exon/intron boundary sequences conform to consensus acceptor (gtn) and donor (nag) sequences, and the exons in the gene appear to encode functional protei ...	1992	1555582
cloning and sequencing of a cdna encoding ascorbate peroxidase from arabidopsis thaliana.	a cdna clone encoding ascorbate peroxidase (ap, ec 1.11.1.11) was isolated from a phage lambda gt11 library of cdna from arabidopsis thaliana by immunoscreening with monoclonal antibodies against the enzyme, and then sequenced. the cdna insert hybridized to a 1.1 kb poly(a)+ rna from leaves of a. thaliana. genomic hybridization suggests that the cdna obtained here corresponds to a single-copy gene. the n-terminal amino acid sequence of arabidopsis ap was determined by protein sequencing of the i ...	1992	1558944
expression and characterization of human mitochondrial ferredoxin reductase in escherichia coli.	ferredoxin reductase (fd-reductase) supplies reducing equivalents obtained from nadph to mitochondrial cytochrome p450 enzymes via the small iron-sulfur protein ferredoxin. two cdnas (differing by the presence or absence of an 18-bp insert in the coding region) for the human fd-reductase were subcloned into a newly constructed general purpose expression vector, p delta blue; protein expression under control of the bacteriophage lambda pl promoter was then induced in escherichia coli. western blo ...	1992	1567230
cloning and nucleotide sequence of the escherichia coli cytidine deaminase (ccd) gene.	the structural gene that encodes cytidine deaminase (cdd) in escherichia coli was cloned from kohara phage lambda 365 (7f1), and its nucleotide sequence was determined. plasmids harboring the gene complemented chromosomal cdd mutations, enhanced cytidine deaminase activity in cell extracts, and directed the synthesis of a protein identical in mass and n-terminal amino acid sequence with cytidine deaminase purified from wild-type bacteria. metal analysis of the purified, plasmid-encoded deaminase ...	1992	1567863
metacyclic form-specific variable surface glycoprotein-encoding genes of trypanosoma (nannomonas) congolense.	a complementary dna expression library in phage lambda gt11 was synthesized using mrna from in vitro-produced metacyclic forms of a clone of trypanosoma (nannomonas) congolense. the unamplified library was screened with antiserum from a goat immune to infection with metacyclic (m)-forms of t. congolense ilrad nannomonas antigen repertoire 2(ilnar2). of the 100 antiserum-reactive phage clones identified, 22 were analyzed further: 21 of the clones contained overlapping portions of a single transcr ...	1992	1572537
somatic cell hybrid deletion map of human chromosome 18.	the creation of a physical map of chromosome 18 will be useful for the eventual identification of specific chromosomal regions that are critical in the occurrence of edwards syndrome, the 18q- syndrome, and the 18p- syndrome. to begin the investigation of these syndromes, a physical map has been constructed to order random dna fragments to specific portions of chromosome 18. a set of somatic cell hybrids that retain deletions or translocations involving chromosome 18 has been isolated and charac ...	1992	1577474
organization, polymorphism, and molecular cytogenetics of chromosome-specific alpha-satellite dna from the centromere of chromosome 2.	the general usefulness of alpha-satellite dna probes for the molecular, genetic, and cytogenetic analysis of the human genome is enhanced by their being chromosome specific. here, we describe the isolation and characterization of an alpha-satellite subset specific for human chromosome 2. three clones, p2-7, p2-8, and p2-11, obtained from an ecori-digested lambda phage library from flow-sorted chromosome 2, are specific for the centromere of chromosome 2 by somatic cell hybrid mapping and chromos ...	1992	1577477
sequence of the bacteriophage sp01 gene 30.	the bacteriophage sp01 gene 30, whose function is essential for dna synthesis, has been analyzed for its primary structural features. conditionally lethal mutations in the gene 30 locus have been mapped and sequenced, and the wild-type amino acid (aa) sequence has been deduced along with that of a co-transcribed and possibly co-translated upstream unidentified reading frame (urf). the aa sequence deduced for gene 30 shares partial similarity with protein p of bacteriophage lambda, which particip ...	1992	1587473
compilation of dna sequences of escherichia coli (update 1992).	we have compiled the dna sequence data for e. coli available from the genbank and embl data libraries and over a period of several years independently from the literature. this is the fourth listing replacing and increasing the former listings substantially. however, in order to save space this printed version contains dna sequence information only, if they are publically available in electronic form. the complete compilation including a full set of genetic map data and the e. coli protein index ...	1992	1598239
characterization of a rabbit gene encoding a clofibrate-inducible fatty acid omega-hydroxylase: cyp4a6.	cyp4a6 mrnas are induced in the rabbit liver and kidney following treatment with the antihyperlipidemic drug clofibrate. as a first step toward the elucidation of the mechanism controlling the induction of this and other cyp4a genes by clofibrate and other peroxisome proliferators, we have cloned and characterized the cyp4a6 gene. genomic dna containing the first 12 exons encoding cyp4a6 was isolated as three recombinant lambda phage, two of which were overlapping. the sequence of more than 1000 ...	1992	1605646
cloning and overexpression of the lactobacillus bulgaricus nad(+)-dependent d-lactate dehydrogenase gene in escherichia coli:purification and characterization of the recombinant enzyme.	the lactobacillus bulgaricus nad(+)-dependent d-lactate dehydrogenase gene was amplified by the polymerase chain reaction and cloned into an escherichia coli expression plasmid pkk223.3. attempts to clone the full-length chromosomal dna encoding d-lactate dehydrogenase from a partial sau3ai lambda phage library or an enriched clone bank in e. coli were unsuccessful. the recombinant plasmid pkbuldh containing the amplified gene overexpressed d-lactate dehydrogenase (greater than 30% of total solu ...	1992	1610363
cloning of a human autoimmune response: preparation and sequencing of a human anti-thyroglobulin autoantibody using a combinatorial approach.	thyroid lymphocyte rna from a hashimoto patient exhibiting high titre serum igg autoantibodies against thyroglobulin (tg) has been used to construct a fab library in phage lambda. screening of this library with radioiodinated tg has permitted the cloning of an anti-tg antibody (mh52) with an affinity of 4.5 x 10(9) molar-1 as determined by inhibition elisa. sequence analysis showed mh52 to be an authentic antibody of the igg1/k isotype with variable region genes from the vhi and vkiii families i ...	1992	1617110
expression cloning of neurotrophic factors using xenopus oocytes.	we have explored the potential for cloning novel neurotrophic factor cdnas via assay of neurotrophic activities following expression in xenopus oocytes. in this report, we describe the successful application of the method to tract rat ciliary neurotrophic factor (cntf) activity from mrna purified from cultured cells and from mrna synthesized by in vitro transcription of a cdna library. rat c6 glioma cells, which had been previously shown to have cntf-like activity (westermann et al., 1988), were ...	1992	1629943
characterization of the promoter region of the porcine opn (osteopontin, secreted phosphoprotein 1) gene. identification of positive and negative regulatory elements and a 'silent' second promoter.	osteopontin (secreted phosphoprotein-1, opn) is a phosphorylated glycoprotein expressed by transformed cells, macrophages, activated t-lymphocytes, specialized epithelial cells and bone cells that is characteristically enriched in milk and in the mineralized matrix of bone. the synthesis of opn by bone cells is regulated by glucocorticoids and growth factors, which promote bone formation, and by the osteotropic hormone calcitriol (1,25-dihydroxycholecalciferol) and retinoic acid, which mediate b ...	1992	1633816
chromosomal localization of human o6-methylguanine-dna methyltransferase (mgmt) gene by in situ hybridization.	recombinant lambda phage dna containing segments of human o6-methylguanine-dna transferase gene was employed to localize this gene among the human chromosomes using non-radioactive in situ hybridization technique. this gene was found to be present at the telomeric end, 26q, of the long arm of the chromosome 10.	1992	1635460
genomic organization, sequence analysis, and chromosomal localization of the human carboxyl ester lipase (cel) gene and a cel-like (cell) gene.	the gene encoding human carboxyl ester lipase (cel), including 1628 bp of the 5'-flanking region, has been isolated and characterized from two overlapping lambda phage clones. the gene spans 9832 bp and contains 11 exons interrupted by 10 introns. the exons range in size from 88 to 204 bp, except for the last exon, which is 841 bp. a major and a minor transcription initiation site were determined 13 and 7 bp, respectively, upstream of the initiator methionine. the nucleotide sequence is identica ...	1992	1639390
geometric differences allow differential enzymatic inactivation of pcr product and genomic targets.	the value of the polymerase chain reaction (pcr) is markedly limited by the ease of carry-over contamination. we predicted that the location of the target sequence which spans the linear center of the molecule in pcr products, but not in genomic molecules, would allow digestion by certain exonucleases (exo). this would eliminate amplifiable targets specifically from pcr products and do so without the need to control either the size of the genomic molecules or the extent of the digestion reaction ...	1992	1644300
the essential escherichia coli msgb gene, a multicopy suppressor of a temperature-sensitive allele of the heat shock gene grpe, is identical to dape.	the grpe gene product is one of three escherichia coli heat shock proteins (dnak, dnaj, and grpe) that are essential for both bacteriophage lambda dna replication and bacterial growth at all temperatures. in an effort to determine the role of grpe and to identify other factors that it may interact with, we isolated multicopy suppressors of the grpe280 point mutation, as judged by their ability to reverse the temperature-sensitive phenotype of grpe280. here we report the characterization of one o ...	1992	1644751
cyclic amp inhibits and putrescine represses expression of the spea gene encoding biosynthetic arginine decarboxylase in escherichia coli.	the spea gene of escherichia coli encodes biosynthetic arginine decarboxylase (adc), the first of two enzymes in a putrescine biosynthetic pathway. the activity of adc is negatively regulated by mechanisms requiring cyclic amp (camp) and camp receptor protein (crp) or putrescine. a 2.1-kb bamhi fragment containing the spea-metk intergenic region, spea promoter, and 1,389 bp of the 5' end of the spea coding sequence was used to construct transcriptional and translational spea-lacz fusion plasmids ...	1991	1646785
direct and general selection for lysogens of escherichia coli by phage lambda recombinant clones.	we report a simple in vivo technique for introducing an antibiotic resistance marker into phage lambda. this technique could be used for direct selection of lysogens harboring recombinant phages from the kohara lambda bank (a collection of ordered lambda clones carrying escherichia coli dna segments). the two-step method uses homologous recombination and lambda dna packaging to replace the nonessential lambda dna lying between the lysis genes and the right cohesive (cos) end with the neomycin ph ...	1991	1646787
the gene encoding cytochrome c oxidase subunit ii from rhodobacter sphaeroides; comparison of the deduced amino acid sequence with sequences of corresponding peptides from other species.	the gene (coxii) encoding subunit ii of rhodobacter sphaeroides cytochrome c oxidase (cytochrome aa3) has been isolated by screening a genomic dna library in phage lambda with a probe derived from coxii of paracoccus denitrificans. a 2-kb fragment containing coxii dna was subcloned into the phage m13mp18 and the sequence determined. the 2-kb insert contains the entire coding region for coxii gene, including the atg start codon and a tga stop codon. the deduced amino acid (aa) sequence of subunit ...	1991	1648008
densitometric determination of human papillomavirus dna quantities by chromato-scanning in the fluorescence mode.	the quantitation of human papillomavirus dna isolated from warts by chromato-scanning (fluorescence mode) photographs of ethidium bromide-stained agarose gels is described. excitation at 200 nm (with a cutoff filter at 400 nm) generates fluorescence from the white portion of the printing paper. the fluorescent intensity correlated with the quantities of dna in the band of interest. the amounts of dna were determined using calibration curves of approximately the same size as lambda phage dna frag ...	1991	1648568
biochemical and genetic analysis of streptococcus mutans alpha-galactosidase.	the aga gene coding for alpha-galactosidase in streptococcus mutans was detected in a recombinant gene library constructed in phage lambda. the gene was subcloned into plasmid vectors and shown to specify a novel protein of mr 80,000. characterization of alpha-galactosidase from s. mutans and from recombinant escherichia coli expressing aga indicated that the enzyme functions as a tetramer. the amino acid composition of the alpha-galactosidase, deduced from nucleotide sequencing of aga, gave a p ...	1991	1649890
a combination of rnase h (rnh) and recbcd or sbcb mutations in escherichia coli k12 adversely affects growth.	colony forming ability of escherichia coli strains carrying the rnh-339::cat mutant allele is strongly dependent on the recbcd and sbcb genes. a mutation inactivating either the recbcd nuclease or exonuclease i (sbcb) is sufficient to restrict severely the efficiency of plating of strains carrying the rnh-339::cat mutation. combining a non-lethal temperature-sensitive mutation in the recbcd nuclease, recb270 (ts) or recc271 (ts), with rnh-339::cat renders strains temperature sensitive for growth ...	1991	1650908
the location of mrna in the ribosomal 30s initiation complex; site-directed cross-linking of mrna analogues carrying several photo-reactive labels simultaneously on either side of the aug start codon.	messenger rna molecules 30-35 bases long, with sequences related to the 5'-region of cro-mrna from lambda-phage, were prepared by t7 transcription from synthetic dna templates. each mrna contained five or six internal uridine residues, which were transcribed using a mixture of utp and thio-utp. initiation complexes were formed with escherichia coli 30s ribosomes in the presence or absence of trna(fmet), and cross-linking of the thio-u residues was induced by uv irradiation at wavelengths greater ...	1991	1651232
characterization of the dna polymerase gene of human herpesvirus 6.	the construction of a recombinant bacteriophage lambda library containing overlapping clones covering 155 kbp of the 161-kbp genome of the ugandan u1102 isolate of human herpesvirus 6 (hhv-6) is described. the use of degenerate-primer polymerase chain reaction allowed the isolation of a dna probe for the dna polymerase gene of hhv-6, which was subsequently used to isolate and position the pol gene on the physical map of the viral genome. a 4.4-kbp ecori dna restriction fragment containing the po ...	1991	1651403
curved dna fragments display retarded elution upon anion exchange hplc.	restriction fragments containing established curved regions, such as the pbr322 tn3 region, the phage lambda attp region and the sv40 t-antigen and terminus of replication regions, exhibit systematically retarded elution upon anion exchange based hplc. using this method, we were able to detect readily other sv40 curved regions, exhibiting also the polyacrylamide gel electrophoresis retardation anomaly, including several rna polymerase initiation sites. unlike gel retardation, hplc retardation ex ...	1991	1651480
enhanced recombination associated with the presence of insertion and deletion mutations in poxvirus-infected cells.	genetic crosses were performed in cells infected by shope fibroma virus in order to determine whether poxviral recombination is influenced by the types of mutations participating in a cross. infected cells were cotransfected with marked bacteriophage lambda dnas and, after dna recovery, the substrates were packaged into infectious particles using in vitro packaging extracts. this made it possible to analyze the products of poxviral recombination systems using lambda mutants plated on escherichia ...	1991	1651592
tn5cos: a transposon for restriction mapping of large plasmids using phage lambda terminase.	a method for the rapid restriction mapping of large plasmids has been developed. a 400-bp fragment of phage lambda dna containing the cos region has been inserted into tn5. after in vivo transposition of this tn5cos element into the plasmid of choice, the plasmid is isolated and linearized at its cos site with phage lambda terminase (ter). such ter linearization was about 70% efficient. after partial digestion of the linear molecules with the appropriate restriction enzyme, the products are sele ...	1991	1652543
the rat interleukin-5 gene: characterization and expression by retroviral gene transfer and polymerase chain reaction.	the rat interleukin-5 (il-5) gene was isolated from a genomic lambda phage library and a fragment containing all four exons was inserted into the retroviral vector pxt1, resulting in pxtril5. upon retroviral gene transfer into two il-5-dependent mouse cell lines, b13 and t88m, autonomously growing cells were established and b-cell growth factor activity was detected in the supernatants of the infected cells. "cdna" versions of the rat il-5 gene were rescued by the polymerase chain reaction (pcr) ...	1991	1653053
the functional bpv-1 e2 trans-activating protein can act as a repressor by preventing formation of the initiation complex.	the products encoded by the e2 open reading frame of the papillomaviruses are dna-binding transcription factors involved in the positive or negative regulation of multiple viral promoters. to further understand the mechanisms by which the same transcription factor may act differentially, the full-length bpv-1 e2 protein was expressed and purified from yeast and assayed in vitro for its capacity to modulate transcription. e2 stimulated transcription of the hsv thymidine kinase (tk) promoter when ...	1991	1653173
seroreactive recombinant herpes simplex virus type 2-specific glycoprotein g.	the herpes simplex virus type 2 (hsv-2) genome codes for an envelope protein, glycoprotein g (gg), which contains predominantly type 2-specific epitopes. a portion of this gg gene has been expressed as a fusion protein in escherichia coli. expression was regulated by a lambda phage pl promoter. the 60,000-molecular-weight recombinant protein was purified by ion-exchange chromatography. amino acid sequence analysis confirmed the n terminus of the purified protein. mice immunized with recombinant ...	1991	1653787
different mechanisms for sos induced alleviation of dna restriction in escherichia coli.	the alleviation of dna restriction during the sos response in escherichia coli has been further investigated. with the ecok dna restriction system uv irradiated wild-type cells show a 10(4)-fold increase in ability to plate non-modified lambda phage and a 3-4 fold increase in transformation by non-modified plasmid dna. a role for the umudc genes of e coli in the process of sos-induced restriction alleviation was identified by showing that a umuc122::tn5 mutant could alleviate ecok restriction to ...	1991	1655051
effect of rfah (sfrb) and temperature on expression of rfa genes of escherichia coli k-12.	in order to study the regulation of a large block of contiguous genes at the rfa locus of escherichia coli k-12 which are involved in synthesis and modification of the lipopolysaccharide core, the transposon tnlacz was used to generate in-frame lacz fusions to the coding regions of five genes (rfaq, -g, -p, -b and -j) within this block. the beta-galactosidase activity of strains in which these fusions had been crossed into the chromosomal rfa locus was significantly decreased when the rfah11 (sf ...	1991	1655711
identification of a gene, closely linked to dnak, which is required for high-temperature growth of escherichia coli.	we have constructed four deletion derivatives of the cloned dnak gene. plasmid pdd1, in which the last 10 amino acids of the dnak protein have been replaced by three different amino acids derived from the pbr322 vector, was as effective as plasmid pkp31, from which it was derived, in restoring the ability of a dnak null mutant, escherichia coli bb1553, to plate lambda phage and to grow at high temperatures. the other three mutations, involving much larger deletions of the dnak gene, did not rest ...	1991	1655950
alkaline phosphatase fusions in the study of cell division genes.	alkaline phosphatase fusions have been used to analyse plasmid- or phage-carried genes from the two-minute region of the escherichia coli chromosome. these studies have revealed the following: 1) bacteriophage lambda carries two genes for cell envelope proteins, lom and bor, that are expressed in lysogens and probably contribute to the pathogenicity of its e. coli host. 2) the ftsq and ftsl gene products are integral proteins of the cytoplasmic membrane with small cytoplasmic domains and large p ...	1991	1656499
immunoanalysis of ultraviolet radiation induced dna damage and repair within specific gene segments of plasmid dna.	the region-specific heterogeneity of repairing dna damage has been established in several biological systems. a flexible and sensitive approach, based upon dna damage specific antibodies, is described to monitor the repair of specific lesions within discrete genomic segments. membrane transblotted dna restriction fragments are immunoanalyzed for the initial formation and repair of 254 nm radiation induced pyrimidine dimers. sensitivity of dimer immunodetection increases proportional to fragment ...	1991	1657185
heteroduplex dna formation is associated with replication and recombination in poxvirus-infected cells.	poxviruses are large dna viruses that replicate in the cytoplasm of infected cells and recombine at high frequencies. calcium phosphate precipitates were used to cotransfect shope fibroma virus-infected cells with different dna substrates and the recombinant products assayed by genetic and biochemical methods. we have shown previously that bacteriophage lambda dnas can be used as substrates in these experiments and recombinants assayed on escherichia coli following dna recovery and in vitro pack ...	1991	1657705
structure and transcriptional status of bovine syncytial virus in cytopathic infections.	the genomic structure of bovine syncytial virus (bsv), a virus commonly infecting cattle, was examined in order to gain insights into the nature of viral dna (vdna) intermediates and the transcriptional status of the virus in cytopathic infections. in dog cf2th cells, the dna intermediate of bsv was found to exist predominantly as linear unintegrated vdna (uvd) molecules. the uvd molecules were cloned directly from total cellular dna by addition of ecori linkers and subsequent ligation into the ...	1991	1657718
direct and crossover pcr amplification to facilitate tn5supf-based sequencing of lambda phage clones.	the 264 bp mini-transposon tn5supf was constructed to sequence dnas cloned in phage lambda without extensive shotgun subcloning or primer walking. unique sequences near each transposon end serve as primer binding sites, and a supf gene is used to select transposition to lambda. we describe here pcr methods that facilitate tn5supf-based sequencing. in a first pass, insertions are mapped relative to the ends of the cloned fragment using pairs of primers specific for vector dna next to the cloning ...	1991	1659687
a versatile method for integration of genes and gene fusions into the lambda attachment site of escherichia coli.	we have developed a versatile method for integration of modified genes and gene fusions into the bacteriophage lambda attachment site (attb) of the escherichia coli chromosome. the method relies on two components: (1) a dna integration cassette, flanked by multiple restriction enzyme sites, which contains the lambda attp site and, as a selectable marker, the tn5 apha gene conferring kanamycin resistance (kmr); and (2) a plasmid with the lambda int gene transcribed from the tet promoter. a fragme ...	1991	1660428
methods for viral isolation and dna extraction for a penaeid shrimp baculovirus.	procedures for the purification of virions and nucleocapsids of baculovirus penaei (bp) of penaeid shrimp and subsequent extraction of the viral nucleic acid are described. bp-infected hepatopancrata, from two species of shrimp from different geographical locations in the americas, were removed and homogenized in a solution of tn buffer (0.01 m tris-hcl, 0.10 m nacl, ph 8.0). the homogenized mixture was strained through a 100-mesh screen to remove large pieces of tissue and centrifuged to concen ...	1991	1660489
molecular analysis of the recombination junctions of lambda bio transducing phages.	to examine the mechanism of recombination involved in the formation of specialized transducing phage during the induction of bacteriophage lambda, we have determined the nucleotide sequences of the recombination junctions of lambda bio phages. the results indicate that abnormal excision takes place at many sites on both bacterial and phage genomes and that the recombination sites have short regions of homology (5-14 bp). some of the sequences of the recombination sites were similar to the consen ...	1991	1660569
characterization of the structure and transcription of an ubiquitin fusion gene from maize.	we have identified a maize ubiquitin (ubi) fusion gene (ubf9) by screening a maize w22 genomic phage lambda library with a short (16-nucleotide) oligodeoxyribonucleotide probe derived from the sequence for the extension sequence of a yeast ub13 fusion gene. ubf9 consists of an ub monomer sequence (228 bp long) joined to an extension sequence (237 bp long). the extension sequence encodes a protein of 79 amino acids which shares extensive identity with similar extension aa sequences found in yeast ...	1991	1660830
factors affecting the digestion of restriction endonucleases in situ.	the influence of incubation buffers and glycerol on the enzyme activity of naked dna of lambda phage and mouse, and of mouse chromosomal dna was investigated. the results obtained varied in part from previously known data, but confirmed the importance of these factors in determining the patterns of in situ restriction enzyme digestion so far attributed exclusively to endonuclease activity.	1991	1663864
cloning and direct sequencing from lambda cdna libraries using the polymerase chain reaction: suppressin and the vasopressin receptor as models.	a strategy using the polymerase chain reaction (pcr) to screen a lambda gt11 pituitary cdna library for cdnas encoding suppressin, a putative anti-proliferative protein, and a putative vasopressin receptor is described. the use of this technique will facilitate the demonstration of e.g. the presence of "neuropeptide receptors" on cells of the lymphoid system, confirming the concept of "shared ligands and receptors" by the neuroendocrine and the immune system. neither of the genes encoding the pr ...	1991	1665209
efficient production of biologically active human prolactin in escherichia coli.	to obtain an adequate amount of human prolactin (hprl) for elucidation of the structure-function relationship, we have expressed the hprl cdna in escherichia coli (e. coli) by using a high-expression vector. the vector contained a chimeric gene encoding a fusion of protein a, a peptide sensitive to collagenase digestion and hprl, which was inserted downstream of the right direction promotor of lambda phage. the resulting protein fusion was purified through three column chromatographies of immuno ...	1991	1665826
[amplification of the phage lambda dna sequence by polymerase chain reaction using thermostable dna polymerase].	the efficiency of phage dna amplification by the method of polymerase chain reaction (pcr) with tth dna-polymerase was studied for optimization of pcr conditions. the effect on amplification efficiency of medium ionic strength and ph, the presence of univalent cations, detergents, gelatin, atp, pyrophosphate, sh-reagents and ratio of concentrations of mg and dntps, primers and template was studied. it has been found that a ph optimum for pcr with tth dna-polymerase varies from 8.5 to 9.0. an ion ...	1991	1667541
complete nucleotide sequence of the gene for human heparin cofactor ii and mapping to chromosomal band 22q11.	heparin cofactor ii (hcii) is a 66-kda plasma glycoprotein that inhibits thrombin rapidly in the presence of dermatan sulfate or heparin. clones comprising the entire hcii gene were isolated from a human leukocyte genomic library in embl-3 lambda phage. the sequence of the gene was determined on both strands of dna (15,849 bp) and included 1749 bp of 5'-flanking sequence, five exons, four introns, and 476 bp of dna 3' to the polyadenylation site. ten complete and one partial alu repeats were ide ...	1991	1671335
isolation and regional localization of a large collection (2,000) of single-copy dna fragments on human chromosome 3 for mapping and cloning tumor suppressor genes.	a collection of 2,000 lambda phage-carrying human single-copy inserts (greater than 700 bp) were isolated from two chromosome-3 flow-sorted libraries. the single-copy dna fragments were first sorted into 3p and 3q locations and about 700 3p fragments were regionally mapped using a deletion mapping panel comprised of two human-hamster and two-human-mouse cell hybrids, each containing a chromosome 3 with different deletions in the short arm. the hybrids were extensively mapped with a set of standa ...	1991	1673958
cloning of the clostridium acetobutylicum atcc 824 acetyl coenzyme a acetyltransferase (thiolase; ec 2.3.1.9) gene.	thiolase (acetyl coenzyme a acetyltransferase; ec 2.3.1.9) from clostridium acetobutylicum is a key enzyme in the production of acids and solvents in this organism. the purification and properties of the enzyme have already been described (d. p. wiesenborn, f. b. rudolph, and e.t. papoutsakis, appl. environ. microbiol. 54:2717-2722, 1988). the thl gene encoding the thiolase has been cloned by using primary antibodies raised to the purified enzyme. a bacteriophage lambda embl3 library of c. aceto ...	1991	1685080
effect of in vitro cleavage of apurinic/apyrimidinic sites on bleomycin-induced mutagenesis of repackaged lambda phage.	previous studies have revealed bleomycin to be a potent base-substitution mutagen in repackaged phage lambda. in order to assess the role of apurinic/apyrimidinic (ap) sites in bleomycin-induced mutagenesis, bleomycin-damaged lambda dna was treated with putrescine or endonuclease iv to effect cleavage of bleomycin-induced ap sites. the dna was then packaged, the phage grown in sos-induced e. coli, and the frequency of clear-plaque mutants in the progeny was determined. bleomycin-induced mutagene ...	1990	1689007
immunological activity of covalently linked t-cell epitopes.	immune responses to proteins necessarily involve the recognition by t lymphocytes of a peptide or peptides derived from a protein complexed with a major histocompatibility antigen. the t-cell response of balb/c mice to the bacteriophage lambda ci repressor protein (residues 1-102) is directed predominantly towards the epitope contained within a single peptide encompassing residues 12-26. similar phenomena of immunodominance of a particular peptide have also been observed in other protein systems ...	1990	1689012
participation of rec genes of escherichia coli k 12 in w-reactivation of uv-irradiated phage lambda.	the effect of the recombinational deficiency on w-reactivation of uv-damaged phage lambda was explored. in this paper we show that w-reactivation is reduced by the recb21 and recf143 mutations after bleomycin (bm) and uv treatment. combination of these mutations in the recb21recf143 double mutant blocks w-reactivation completely after bm induction, but leaves residual w-reactivation ability after uv-irradiation, which is abolished by the introduction of uvrb deficiency (delta(uvrb-chla]. w-react ...	1990	1689458
cloning and sequencing of protochlorophyllide reductase.	putative protochlorophyllide reductase cdna clones (252 and 113) were isolated from an etiolated-oat (avena sativa) cdna library. these were used to indirectly characterize a further clone, p127, isolated from a lambda-phage gt11 cdna library. the latter (1.15 kb in length) was sequenced, and the derived amino acid sequence was shown to be remarkably similar to that derived from chemical analysis of a cnbr-cleavage fragment of the purified reductase, p127 codes for more than 95% of the reductase ...	1990	1689568
analysis of the structure-function relationship of tumour necrosis factor. human/mouse chimeric tnf proteins: general properties and epitope analysis.	to analyse the structure-function relationship of tumour necrosis factor (tnf), a set of in-frame chimeric genes was constructed by coupling appropriate segments of the human and mouse tnf coding regions. under control of the bacteriophage lambda inducible pl promoter high level expression of these chimeric genes was obtained in escherichia coli. although both human and mouse tnf were produced in e. coli as soluble proteins, a reduction of solubility was observed in some of the chimeric proteins ...	1990	1689779
organization and sequence of the human gene encoding cytokeratin 8.	among the various intermediate filament (if) proteins, cytokeratin 8 (ck8) is especially remarkable as it is produced early in embryogenesis, is the only type-ii ck occurring in many simple epithelial cells, and can also be synthesized in certain non-epithelial cells. using a cdna probe specific for human ck8 we have isolated an approx. 14-kb genomic clone (in phage lambda embl3) which contains the gene encoding human ck8. the gene comprising a total of 7766 nucleotides (nt) from the transcripti ...	1990	1691124
development of a short-term, in vivo mutagenesis assay: the effects of methylation on the recovery of a lambda phage shuttle vector from transgenic mice.	transgenic mice suitable for the in vivo assay of suspected mutagens at the chromosome level have been constructed by stable integration of a lambda phage shuttle vector. the shuttle vector, which contains a beta-galactosidase (beta-gal) target gene, can be rescued from genomic dna with in vitro packaging extracts. mutations in the target gene are detected by a change in lambda phage plaque color on indicator agar plates. initial rescue efficiencies of less than 1 plaque forming unit (pfu)/100 m ...	1990	1693420
marek's disease virus gene clones encoding virus-specific phosphorylated polypeptides and serological characterization of fusion proteins.	marek's disease virus (mdv) gene clones, ra2 and ga8, constructed in e. coli bacteriophage lambda-gt11 (gt11) were identified by a monoclonal antibody (mab), h19.47, against a putative transformation-related viral antigen consisting of a complex of three phosphorylated polypeptides, pp41, pp38, and pp24. both recombinants have a mdv-dna insert of about 0.5 kb and are mapped to the region of bamhi-h or ecori-x fragments of the mdv genome by southern blot hybridization. immunoblot and immunoprecip ...	1990	1693456
regulation of the mouse alpha a-crystallin gene: isolation of a cdna encoding a protein that binds to a cis sequence motif shared with the major histocompatibility complex class i gene and other genes.	we have shown by site-directed mutagenesis that the sequence between positions -69 and -40 of the mouse alpha a-crystallin gene is crucial for tissue-specific gene expression in a transfected mouse lens epithelial cell line transformed with the early region of simian virus 40. gel retardation experiments with synthetic oligodeoxynucleotides revealed a mouse lens nuclear protein which bound specifically to the palindromic sequence 5'-gggaaatccc-3' at positions -66 to -57 in the alpha a-crystallin ...	1990	1694016
the use of lambda phage shuttle vectors in transgenic mice for development of a short term mutagenicity assay.		1990	1697073
temperature-inducible gene expression in bacillus subtilis mediated by the ci857-encoded repressor of bacteriophage lambda.	an efficient system to control the expression of cloned genes in bacillus subtilis was established by introducing the escherichia coli bacteriophage lambda ci857 repressor-pr promoter system into this host. a staphylokinase reporter gene (sak42d), which was fused to the lambda pr promoter was constitutively expressed in b. subtilis even when the ci857 gene was present on the same plasmid. s1 nuclease mapping of the transcription start point confirmed that the pr promoter was active in b. subtili ...	1990	1699846
cloning and expression of n-acetylglucosaminyltransferase i, the medial golgi transferase that initiates complex n-linked carbohydrate formation.	this laboratory has previously identified a human gene encoding n-acetylglucosaminyltransferase i (glcnac-ti; ec 2.4.1.101) by complementation of the glycosylation defect in the lec1 chinese hamster ovary (cho) cell mutant. a phage lambda library prepared from genomic dna of a tertiary lec1 transfectant (3 degrees t) has now been used to obtain clones encoding an active glcnac-ti enzyme. a small genomic dna fragment [approximately 4.6 kilobases (kb)], isolated from an alupositive lambda clone, c ...	1990	1702225
biological characterization of infectious molecular clones derived from a human immunodeficiency virus type-1 isolate with rapid/high replicative capacity.	in order to molecularly characterize rapidly and slowly replicating hiv-1 variants, molecular clones were obtained from a rapid/high virus isolate. this isolate, 4803, had only been passaged in peripheral blood mononuclear cells (pbmc) prior to cloning. molecular cloning was done in bacteriophage lambda-dash using high molecular weight dna of isolate 4803 infected pbmc. seven recombinant phages were identified. the clones were found to be related to each other and differed only at 1 or 2 restric ...	1991	1704660
characterization of int-5, a locus associated with early events in mammary carcinogenesis.	three chemically-induced precancerous mammary hyperplasias, independently isolated in balb/c mice, all contained mouse mammary tumor virus (mmtv) proviral dna integrated into a common region in chromosomal dna, designated int-5 (formerly int-h, gray et al., 1986). this site was cloned from a hyperplastic outgrowth (d2) into lambda phage. a 1.7 kb hind iii dna fragment, which flanks the 5' end of the mmtv insert, was generated from the cloned int-5 region. this fragment was used as probe (ih-2) t ...	1991	1705320
construction of a uniform-abundance (normalized) cdna library.	we have used a kinetic approach to construct cdna libraries containing approximately equal representations of all sequences in a preparation of poly(a)+ rna. randomly primed cdna fragments of a selected size range were cloned in lambda phage vector. inserts were amplified by the polymerase chain reaction (pcr), denatured, and self-annealed under optimized conditions. after extensive but incomplete reannealing, the single-stranded fraction was relatively depleted of more abundant species of cdna. ...	1991	1705712
structure of the gorilla alpha-fetoprotein gene and the divergence of primates.	the sequence of the gorilla alpha-fetoprotein gene, including 869 base pairs of the 5' flanking region and 4892 base pairs of the 3' flanking region (24,607 in total), was determined from two overlapping lambda phage clones. the sequence extends 18,846 base pairs from the cap site to the polyadenylation site, and it reveals that the gene is composed of 15 exons, which are symmetrically placed within three domains of alpha-fetoprotein. the deduced polypeptide chain is composed of a 19-amino-acid ...	1991	1706310
structure of cdnas encoding the triple-helical domain of murine alpha 2 (vi) collagen chain and comparison to human and chick homologues. use of polymerase chain reaction and partially degenerate oligonucleotide for generation of novel cdna clones.	type vi collagen cdnas of human and avian origin were recently obtained and characterized by screening cdna libraries in lambda phage. based on the published sequences of these cdnas, we constructed partially degenerate oligonucleotide primers that we used in polymerase chain reactions for the generation of alpha 2(vi) collagen clones of murine origin. as template, we used cdna derived from murine total rna. we amplified, cloned and sequenced a 1043-bp fragment that contains the coding sequence ...	1991	1709252
the manipulation of dna with restriction enzymes in low water systems.	the cleavage of phage lambda (lambda) dna by the restriction enzyme hindiii in low water systems has been investigated. two types of low water systems have been studied--those which contain a surfactant in a reverse micelle environment and a surfactant-free system in which a solid support (celite) is used. the effect of the surfactants themselves in a normal aqueous environment has also been studied. charged surfactants were found to greatly inhibit hindiii activity in aqueous buffer, while non- ...	1991	1710498
genetic studies of cleavage-initiated mrna decay and processing of ribosomal 9s rna show that the escherichia coli ams and rne loci are the same.	we show in the present paper that the cleavages initiating decay of the ompa mrna are suppressed both in the escherichia coli ams(ts) strain (originally defined by a prolonged bulk mrna half-life) and in the me(ts) strain (originally defined by aberrant 9s rna processing). the temperature-sensitive defects of both these strains are complemented by a recombinant lambda phage containing a genomic segment that carries the putative ams locus. a 5.8 kb fragment from this genomic dna segment was clone ...	1991	1713283
identification of a cloned sequence activated during multi-stage carcinogenesis in mouse skin.	differential screening of cdna libraries made from chemically induced malignant mouse skin squamous cell carcinomas (scc) identified three sequences, including one called mal2, that were upregulated in their expression at both the benign papilloma and malignant scc stages. the mal2 plasmid cdna clone (containing a 350 bp insert) was used to screen lambda phage cdna libraries made from chemically induced sccs. two of the largest mal2-related cdna inserts obtained from the phage libraries were seq ...	1991	1713533
overexpression of the phage lambda lysozyme cloned in escherichia coli: use of a degenerative mixture of synthetic ribosome binding sites and increase of the protein stability in vivo.	the r gene of the phage lambda coding for a lysozyme expressed at the end of an infection cycle in escherichia coli has been cloned in a series of vector plasmids. two methods for improving the efficiency of translation have been tested. first, the use of a bicistronic construction in which the ribosome binding site (rbs) of the first cistron is that of a highly expressed gene or the use of a degenerate mixture of synthetic oligonucleotides for the optimization of a rbs. the second strategy is m ...	1991	1715562
development-specific genes and their expression at the rna level.	a total of seven clones, which are specific for the development of carrot somatic embryo cells, were constructed and screened from the cdna expression library of phage lambda gt11. the purification of plaques, isolation of dna from the phages, digestion of the clone's dna with ecori restriction enzyme, identification of the inserts on the agarose gel by electrophoresis and subcloning of cdna inserts from phage lambda gt11 vector into plasmid pibi or puc18 were performed respectively. the data of ...	1990	1717019
efficient templates for q beta replicase are formed by recombination from heterologous sequences.	a very efficient replicase template has been isolated from the products of spontaneous rna synthesis in an in vitro q beta replicase reaction that was incubated in the absence of added rna. this template was named rq135 rna because it is 135 nucleotides in length. its sequence consists entirely of segments that are homologous to ribosomal 23 s rna and the phage lambda origin of replication. the sequence segments are unrelated to the sequence of q beta bacteriophage genomic rna. nonetheless, this ...	1991	1717699
specificity of antitermination mechanisms. suppression of the terminator cluster t1-t2 of escherichia coli ribosomal rna operon, rrnb, by phage lambda antiterminators.	transcription of the ribosomal rna operons (rrn) in escherichia coli is subject to an antitermination mechanism whereby rna polymerase is modified to a termination-resistant form during transit through the rrn leader region. this antitermination mechanism is unable to overcome the t1-t2 terminator cluster located at the end of an rrn operon, such as rrnb. we have tested the specificity with which the t1-t2 terminators override an antitermination mechanism, by placing the terminator cluster downs ...	1991	1719220
rho-dependent transcription termination. characterization of the requirement for cytidine in the nascent transcript.	by substituting template segments encoding au-rich, gu-rich, and ca-rich transcripts for natural sequences upstream of the phage lambda rho-dependent tr1 termination site, we demonstrate that cytidines are required in the upstream rna for rho-dependent termination to occur. these results are extended through in vitro mutagenesis of a template encoding an inactive au-rich upstream sequence: certain mutant templates encoding new cytidines are able to activate rho-dependent termination. cytidines m ...	1991	1721066
tagging the genome of the murine leukemia retrovirus sl3-3 by a bacterial lac operator sequence.	the bacterial lactose operator (laco) was introduced into the psti site of the long terminal repeat of the sl3-3 murine leukemia virus, generating a virus, sl3-3laco, that can replicate in nih3t3 cell cultures. dna sequences harboring the laco sequence might be recovered by molecular cloning in escherichia coli lac+ lacz+ using bacteriophage lambda or plasmid vectors. the high copy numbers of the laco sequence titrate out the lac repressor, leading to the induction of the lac operon in the host. ...	1991	1722473
genetic mapping of starch- and lambda-receptor sites in maltoporin: identification of substitutions causing direct and indirect effects on binding sites by cysteine mutagenesis.	cysteine mutagenesis was used to test the proximity of 16 residues to protein-ligand interaction sites in maltoporin (lamb protein). lamb protein with additional cysteines was incorporated into the outer membrane of escherichia coli except with a ser-30----cys substitution. phage lambda and starch binding was assayed before and after incubation of mutants with six thiol-specific reagents. four categories of mutation were recognized on the basis of phenotype and modification for each of the lambd ...	1991	1722561
a novel approach to nonradioactive hybridization assay of nucleic acids using stained latex particles.	the paper describes a sensitive latex hybridization assay (lha) method applied for indirect detection of biotinylated nucleic acid hybrids immobilized on a synthetic membrane. the biotinylated hybrids were visualized by means of latex particles containing the fluorescent dye pyronine g and coated with streptavidin; 1.6 and 0.3 pg of lambda-phage dna was detected by dot blot hybridizations on nylon membrane and polyethyleneimine-cellophane, respectively. the assay sensitivity was increased by thr ...	1991	1724721
ribosomal rna and peptidyl-trna hydrolase: a peptide chain termination model for lambda bar rna inhibition.	we propose here a model to explain the inhibition of bacteriophage lambda (lambda) vegetative growth and the killing of e coli cells defective in peptidyl-trna hydrolase (pth) by lambda bar rna. the model suggests that bar rna, which contains a characteristic uga triplet, base-pairs in an anti-parallel fashion with the 1199-1205 region of e coli 16s rrna. in doing so, it prevents the required functioning of that region of 16s rrna in uga-specific peptide chain termination. pth is implicated in p ...	1991	1725266
regulation of the salmonella typhimurium meta gene by the metr protein and homocysteine.	the dna sequence of the salmonella typhimurium meta control region is presented. s1 nuclease mapping was used to determine the transcription initiation site. by measuring beta-galactosidase levels in escherichia coli strains lysogenized with lambda phage carrying a meta-lacz gene fusion, the metr protein was shown to activate the meta gene. homocysteine, an intermediate in methionine biosynthesis, plays a negative role in the metr-mediated activation mechanism. gel mobility shift assays and dnas ...	1992	1729233
renaturation of cobra venom phospholipase a2 expressed from a synthetic gene in escherichia coli.	cobra venom (naja naja naja) phospholipase a2 (pla2) contains 14 cysteines in the form of 7 disulfide bonds amongst its 119 amino acids. a gene encoding the pla2 was synthesized and inserted into a bacterial expression vector containing the phage lambda pl promoter. in order to obtain protein without the initiating methionine at the n-terminus, a factor xa site was engineered upstream from the pla2 gene. upon heat-induction of the cells transformed with the expression plasmid, the protein is pro ...	1992	1730025
saccharomyces cerevisiae elongation factor 2. genetic cloning, characterization of expression, and g-domain modeling.	the elongation factor 2 (ef-2) genes of the yeast saccharomyces cerevisiae have been cloned and characterized with the ultimate goal of gaining a better understanding of the mechanism and control of protein synthesis. two genes (eft1 and eft2) were isolated by screening a bacteriophage lambda yeast genomic dna library with an oligonucleotide probe complementary to the domain of ef-2 that contains diphthamide, the unique posttranslationally modified histidine that is specifically adp-ribosylated ...	1992	1730643
direct interaction between two escherichia coli transcription antitermination factors, nusb and ribosomal protein s10.	the escherichia coli proteins nusb and ribosomal protein s10 are important for transcription antitermination by the bacteriophage lambda n protein. we have used sucrose gradient co-sedimentation and affinity chromatography with immobilized ribosomal protein s10, a glutathione s-transferase-s10 fusion protein, and nusb to show that nusb binds directly and very selectively to s10. the interaction is non-ionic and has an estimated kd value of 10(-7) m. we hypothesize that nusb binds to n-modified t ...	1992	1731086
cloning of drosophila transcription factor adf-1 reveals homology to myb oncoproteins.	the drosophila sequence-specific dna binding protein, adf-1, is capable of activating transcription of the alcohol dehydrogenase gene, adh, and is implicated in the transcriptional control of other developmentally regulated genes. we have cloned the cdna encoding adf-1 by generating specific dna probes deduced from partial amino acid sequence of the protein. several cdna clones encoding an extended open reading frame were isolated from a phage lambda library. the complete amino acid sequence of ...	1992	1731341
structure and expression of the rat epididymal secretory protein i gene. an androgen-regulated member of the lipocalin superfamily with a rare splice donor site.	the complete rat epididymal secretory protein i (esp i) gene was isolated from a genomic library constructed in bacteriophage lambda charon 4a. the complete nucleotide sequence of the gene and its immediate 5' and 3' flanking sequences were determined. interesting features include the presence of a rare, but functional, splice donor site (...gc) and the presence of a putative androgen-receptor-binding element. a detailed analysis of esp i regulation was carried out after castration and subsequen ...	1992	1731756
molecular cloning of the cdna for the major hemoglobin component from paramecium caudatum.	nucleotide sequence of the cdna for the major hemoglobin component of paramecium caudatum was determined. an oligonucleotide was synthesized on the basis of the amino acid sequence, and the paramecium cdna library constructed in phage lambda gt11 was screened with it. three positive clones, of which insert sizes were 0.4, 0.6, and 0.9 kbp, were obtained. sequence analysis made clear that the 0.4-kbp cdna retains a full length of the nucleotides encoding 116 amino acid residues, and that in the c ...	1992	1731779
identification of the template binding polypeptide in the pea chloroplast transcriptional complex.	we have identified the template-binding polypeptide in the pea chloroplast transcriptional complex by photoaffinity labelling. this polypeptide has an apparent molecular weight of about 150 kda and binds to both, chloroplast ribosomal (16s rrna) and messenger (psba) promoters. the 16s rrna and psba promoters were amplified from chloroplast dna by the polymerase chain reaction and labelled with a photoactive analogue of ttp, 5-bromodeoxy utp, as well as with alpha-32p-dctp. using the filter-bindi ...	1992	1738606
a polymorphic (ca)n repeat element maps the human glucokinase gene (gck) to chromosome 7p.	a compound imperfect dinucleotide repeat element, [ca]4tttgt[ct]7[ca]9aa[ca]4ccacata[ca]3, was found approximately 10 kb 3' to the human glucokinase gene (gck) from analysis of contiguous genomic dna obtained from a bacteriophage lambda chromosome walk. direct human genomic sequencing revealed the source of polymorphism to be variable numbers of ct and ca repeats. altogether six alleles that range in length from +10 to -15 nucleotides compared to the most common (z) allele have been identified. ...	1992	1740341
multiple origins of the human glycophorin sta gene. identification of hot spots for independent unequal homologous recombinations.	human glycophorin sta (hgpsta), one of the structural variants of erythrocyte membrane sialoglycoproteins, is encoded by a delta-alpha hybrid gene that arose from a single unequal crossover between the parent hgpb(delta) and hgpa(alpha) genes. we report here the identification of two new hgpsta genes (type a and type b) in four unrelated sta heterozygotes from two ethnic groups. these sta genes represent distinct genetic isoforms that differ from the previously reported sta gene (type c) in the ...	1991	1744126
a species-specific oligonucleotide dna probe for the identification of meloidogyne incognita.	a genomic library of meloidogyne incognita race 1 has been prepared in the bacteriophage lambda gt10 and screened for specific dna sequences by hybridization with radio-isotope labelled total genomic dna from a number of meloidogyne species. one clone isolated (mr1 #15), although not totally species specific, clearly showed preferential hybridization to m. incognita. following subcloning and sequencing of the 255 bp insert, four stretches of the sequence corresponding to oligonucleotides of appr ...	1991	1745557