Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| isolation of the transfer rna genes of bacteriophage t4 and transfer rna synthesis in vitro. | non-glucosylated t4 dna was restricted with the endonuclease ecori and the mixture of dna fragments separated by gel electrophoresis and transcribed with purified escherichia coli rna polymerase. three purified fragments were shown to act as templates for trna synthesis. a smaller fragment, shown to be hybridizable to 32p-labeled t4 trna was not transcribable. it was concluded that the promoter for t4 trna synthesis had been separated from the structural genes in the smaller fragment by ecori an ... | 1979 | 420849 |
| sequential effects of thyroxine on the developing cerebellum of rats made hypothyroid by propylthiouracil. | young rats made hypothyroid by propylthiouracil (ptu) received a daily physiological dose of thyroxine (t4) from day 0, 4, 6, 8, 10, 11, 12 or 13 and their cerebella were studied on day 14. with the very low doses of t4 used and when the treatment was started at birth, cerebellar development was nearly normal in terms of the parameters studied (cell formation, migration, maturation and death). the effect of t4 on cell formation appeared after two days. with the same latency, t4 induced migration ... | 1979 | 421132 |
| error induction and correction by mutant and wild type t4 dna polymerases. kinetic error discrimination mechanisms. | the fidelity of dna synthesis as determined by the misincorporation of the base analogue 2-aminopurine in competition with adenine has been measured as a function of deoxynucleoside triphosphate substrate concentrations using purified mutator (l56), antimutator (l141), and wild type (t4d) t4 dna polymerases. although the rates of both incorporation and turnover of aminopurine and adenine decrease as substrate concentrations are decreased, the ratio of turnover/polymerase activity is increased. t ... | 1979 | 422561 |
| serum myoglobin level in altered thyroid states. | serum myoglobin (mb) levels were measured in normal, hyperthyroid, and hypothyroid subjects by ria. serum mb levels were significantly higher (p less than 0.001) in untreated hypothyroid patients and significantly lower (p less than 0.01) in untreated hyperthyroid patients than in normal subjects. there was a significant inverse correlation (p less than 0.01) between serum t4 or t3 levels and mb levels in these subjects. serum mb levels were normalized after correction of the abnormal thyroid st ... | 1979 | 422690 |
| molecular basis of thermostability in the lysozyme from bacteriophage t4. | 1979 | 423967 | |
| the iodine content of amniotic fluid and placental transfer of iodinated drugs. | determinations of total iodine content, levels of thyroxine (t4) and triiodothyronine (t3), and protein concentrations were made on amniotic fluid. a total of 218 samples obtained at various stages of pregnancy, from both normal and pathological pregnancies, were studied. normal values for the iodine and hormone concentrations are presented. several amniotic fluid samples showed greatly elevated iodine levels, thought to be the result of maternal iodine intake. this study demonstrated that such ... | 1979 | 424113 |
| a new method for 3'-labelling of polyribonucleotides by phosphorylation with rna ligase and its application to the 3'-modification for joining reactions. | p1-adenosine 5'-p2-o-nitrobenzyl pyrophosphate (nbzlppa) has been synthesized as a substrate for t4 rna ligase catalyzed 3'-phosphorylation. incubation of oligoribonucleotides and nbzlppa with rna ligase yielded oligoribonucleotides having a 3'-o-(o-nitrobenzyl) phosphate. photochemical removal of the o-nitrobenzyl group provided the free 3'-phosphate. using [p2-32p] nbzlppa, 3'-termini of oligoribonucleotides could be labelled with 32p. this reaction was applied to modify the 3'-end of donor mo ... | 1979 | 424301 |
| [problems of a stage-based therapeutic approach to surgery in breast carcinoma (author's transl)]. | a therapeutic concept dependent on staging of breast carcinoma is presented: 1. stage t0-t1 n0 m0: wide local excision or quadrant resection with axillary lymph-node excision. 2. stage t2 and t3 n0-n1: simple mastectomy (patey). 3. stage t4 or n2: radical mastectomy (rotter). 4. stage n3 or m1: tumour excision according to the t-stage, lymph-node excision, local excision of affected distant lymph nodes. with equal therapeutic results the smaller and cosmetically-preferable surgical procedure is ... | 1979 | 425526 |
| thyroxine uptake and metabolism by fetal sheep after intra-amniotic thyroxine injection. | thyroxine (t4) uptake from amniotic fluid was investigated in fetal sheep. samples of fetal and maternal blood and of amniotic fluid were obtained from indwelling catheters at specific intervals after intra-amniotic injection of t4. t4 and reverse t3 (rt3) were measured by radioimmunoassay. basal levels of t4 were 7.3 +/- 0.92, less than 2, and 6.28 +/- 0.49 microgram/dl in the fetus, amniotic fluid, and ewe, respectively. basal levels of rt3 were 3,858 +/- 214, 189 +/- 62, and 385 +/- 20 pg/ml ... | 1979 | 426016 |
| on model and data requirements for determining the bioavailability of oral therapeutic agents: application to gut absorption of thyroid hormones. | a two-pool model is proposed as a first approximation of the dynamics of thyroid hormone dissolution and absorption in the human gut. it is shown that this model, or any more complex one of the same process, must be imbedded in a larger whole-body model for the purpose of quantifying its parameters. parameter identification experiments are designed with the aid of identifiability analysis and a complete quantification is obtained, for both triiodothyronine (t3) and thyroxine (t4) absorption, usi ... | 1979 | 426090 |
| thyrotropin, free and total triiodothyronine, and thyroxine in serum during surgery. | changes in free and total thyroxine (t4), triiodothyronine (t3) and thyroid stimulating hormone (tsh) in serum were measured before, during, and for nine days after uncomplicated abdominal surgery in eight patients. the results showed a pronounced fall in total as well as in free t3 levels, amounting to 50% in both variables on the first postoperative day, and returning towards preoperative levels on the seventh and ninth day. apart from an increase during surgery, free t4 levels were constant i ... | 1979 | 428113 |
| thyroid hormone levels during glucose tolerance test in euthyroid subjects. | twelve normal, healthy, clinically and biochemically proven euthyroid volunteer subjects (age 19-35) were administered a standard glucose tolerance test (100 g glucose orally) and thyroxine (t4), triiodothyronine (t3) and reverse triiodothyronine (rt3) levels were determined by specific radioimmunoassays to determine the acute effect of glucose and insulin on peripheral monodeiodination of thyroxine. the fasting levels of t4, t3, rt3 and free thyroxine were 82.4 nmol/l, 1.7 nmol/l, 0.52 nmol/l, ... | 1979 | 428118 |
| [serum thyroxine determination with solid phase gamma coat t4 ria kit--experimental and clinical studies]. | 1979 | 428134 | |
| comments on t4 level in patients treated with d--thyroxine. | 1979 | 428344 | |
| a new site-specific endonuclease from streptomyces lavendulae (slai). | a new restriction-like endonuclease, slai, was found and partially purified from streptomyces lavendulae atcc8664. this endonuclease cleaved bacteriophage lambda dna at only one site, and cytosine-substituted bacteriophage t4 dna at 16 sites. the recognition sequence was determined by using slai fragments of cytosine-substituted bacteriophage t4 dna. the hexanucleotide recognized by slai endonuclease was (formula: see text), with the sites of cleavage as indicated by the arrows. therefore, slai ... | 1979 | 428725 |
| serum t4, t3 and reverse t3 during treatment with propranolol in hyperthyroidism, l-t4 treated myxedema and in normal man. | serum concentrations of t4, t3 and reverse t3 were studied in two hyperthyroid groups (n = 13 and 11), in a group of normals (n = 9) and in a group of l-t4 substituted patients (n = 7) with severe pretreatment hypothyroidism. serum t4 did not change except in one of the hyperthyroid groups change to in which a slight decrease was found. in all groups a significant fall in serum t3 and a significant rise in serum reverse t3 were found. an expected increase in serum tsh in the normal and in the l- ... | 1979 | 428906 |
| peptide mapping of heterogeneous protein samples. | a simple two-dimensional electrophoretic method for peptide mapping of heterogeneous protein samples is presented. the reduced and denatured proteins of the mixture are separated in a first dimension by sodium dodecyl sulfate-polyacrylamide slab gel electrophoresis. after completion of the electrophoresis, the whole gel lane is equilibrated in stacking gel buffer and is transferred at right angles onto a second slab gel. a protease solution is overlayed on the gel lane and a partial proteolysis ... | 1979 | 429300 |
| oscillations in reverse triiodothyronine levels in serum of healthy infants aged 0 to 130 hours. | rt3 was measured by ria in sera from 273 normal healthy infants between 0--130 h of age. the curve for the mean rt3 level plotted against age was polyexponential in shape and showed superimposed oscillations with a period of about 16 h, similar to the curves for t4, t3, and tsh previously found for the same group of subjects (j clin endocrinol metab 47: 61, 1978). the ratio of concentrations of rt3 to t4 remained approximately constant, with a mean value of 0.016 over the age range of 3--130 h. ... | 1979 | 429523 |
| comparison of thyroid stimulators and thyroid hormone concentrations in the sera of pregnant women. | to clarify the role of various thyroid stimulators in normal human pregnancy, we measured serum tsh, chorionic tsh (hct), hcg, bioassayable thyroid-stimulating activity, t4, t3, t3 uptake, free t4 and free t3 indexes, free t4, and free t3 by dialysis in 339 serum samples from pregnant women at various intervals of pregnancy and in 40 normal female controls. serum t4 and t3 and free t4 and free t3 indexes were significantly elevated throughout pregnancy in comparison with controls. free t4 concen ... | 1979 | 429524 |
| [the effect of a beta blocker on various manifestations of hypermetabolism and hypersympathicotonia]. | the possible mechanism and the therapeutic activity of a beta-blocking drug (propranolol) in 50 patients with slight "hyperthyroidism" or "dysthyroidism" are examined. in most cases, the laboratory findings were :1) increase (sometimes considerable) of basal metabolism (up to 70% or more); 2) normal, or nearly normal, thyroid scan, iodoproteinaemia, t3 and t4; 3) therapeutic iodine improvement. prior to the introduction of scanning, these cases would have been classed as "hyperthyroidism" or "dy ... | 1979 | 431848 |
| [clinical and hormonal evaluation of recurrence of goiter after thyroidectomy]. | the incidence and features of goitre recurrences were investigated in a sample population of patients operated at the university of turin endocrine surgery centre. the following parameters were examined: sex, age, familial history of goitre, number of pregnancies, t3 and t4 levels, and tsh levels. attention is drawn to the finding of significantly higher tsh values in the patients operated for recurrent goitre than in a series of persons free of recurrences after they had been operated on for go ... | 1979 | 431874 |
| functions of baseplate components in bacteriophage t4 infection. iii. the functional organization of the baseplate. | 1979 | 433148 | |
| selective isolation of a thymine-requiring mutant of phage t4. | 1979 | 433155 | |
| the influence of endogenous cortisol on the peripheral conversion of thyroxine in patients with acute myocardial infarction. | a study was performed to elucidate whether endogenous cortisol, as previously suggested, could be responsible for the decreased t3 levels seen in euthyroid patients with acute myocardial infarction. levels of these hormones as well as levels of t4 and reverse-t3 were monitored in 31 consecutive patients admitted to the coronary care unit with symptoms of precordial pain or with acute arrhythmias. sixteen of the patients had proven myocardial infarction, the remaining 15 were used as a control gr ... | 1979 | 433662 |
| results of a regional cord blood screening programme for detecting neonatal hypothyroidism. | our regional cord blood screening programme for detecting neonatal hypothyroidism using initial cord blood thyroxine (t4) determinations, with supplemental thyrotropin (tsh), and triiodothyronine resin uptake (t3u) measurements, gave an incidence of thyroid abnormalities of 1/3000 births, with 1/5000 infants having severe primary hypothyroidism. no hypothyroid infant detected in the programme had been suspected clinically before the screening and, in retrospect, only a few babies had any signs o ... | 1979 | 434901 |
| l-thyroxine treatment of diffuse non-toxic goitre evaluated by ultrasonic determination of thyroid volume. | the effect of a daily dose of 150 micrograms l-thyroxine for one year was studied in forty-five patients with diffuse non-toxic goitre. thyroid volume was evaluated by ultrasonic scanning. a decrease in the median value of the thyroid volume of about 20% was found after 3 months of therapy and no further change in the median value was observed in the following 9 months of treatment. about 50% of the patients showed a response to therapy and about 30% obtained normal size of the thyroid gland. me ... | 1979 | 436302 |
| [t4 ria by a solid phase method--studies of spac t4 ria kit]. | 1979 | 436324 | |
| the effect of hypothyroidism on growth, serum growth hormone, the growth hormone-dependent somatomedin, insulin-like growth factor, and its carrier protein in rats. | to study the possible mechanisms involved in growth retardation associated with hypothyroidism, serum t4, gh, the gh-dependent somatomedin, insulin-like growth factor (igf), and its carrier protein (cp) were measured in hypothyroid rats and their age-matched controls. three groups of rats were studied: infant, immature, and adult. marked hypothyroidism (serum t4, less than 1 microgram/dl) was produced in experimental animals by providing them with drinking water containing 0.05% propylthiouracil ... | 1979 | 436752 |
| immunoreactive somatostatin in rat hypophysial portal blood. | somatostatin levels have been determined by ria in hypophysial portal blood of pentobarbital-anesthetized male rats. in most animals, immunoreactive somatostatin (srif) levels were higher in hypophysial portal blood than in systemic blood. in euthyroid rats, the mean level was 158 +/- 27 pg/ml (n = 8); srif was undetectable (less than 30 pg/ml) in systemic blood of these rats. it is suggested that endogenous srif was not degraded during the collection of stalk blood, since synthetic srif is stab ... | 1979 | 436785 |
| a search for hapten-binding mouse plasmacytoma proteins. | six hundred and twelve mouse plasmacytomas were screened for hapten binding by using eleven different bacteriophage-hapten conjugates (phage t4 conjugated with haptens np, nip, dip, dnp,boc-aba-tyr, aba-np, aba-mip, abs-hop, pab-hop, penicillin g, cloxacillin). fifteen ascites fluids (2.4%) inactivated at least one of the phage conjugates at a high dilution indicating binding. the specificity of these reactions was studied by titrating one ascites fluid with phage conjugates carring unrelated ha ... | 1979 | 436927 |
| [studies on the measurement of serum thyroxine with "konsul t4 ria" kit (author's transl)]. | 1979 | 439492 | |
| [solid-phase radioimmunoassay of serum thyroxine by using spac t4 ria kit (author's transl)]. | 1979 | 439494 | |
| [thyroid function test by ria kit (solid phase) of spac t4 and spac t3 (author's transl)]. | 1979 | 439495 | |
| proteolytic cleavage and structural transformation: their relationship in bacteriophage t4 capsid maturation. | giant t4 phage capsoids formed in canavanine-treated cultures infected by phage mutants in genes 21 and 17, respectively, differ with regard to cleavage of the major capsid protein, gp23, and in the fine structure of their hexagonal surface lattices. quantitative computer processing of electron micrographs shows that the significant differences in capsomer morphology amount to six symmetrically placed features present in the uncleaved hexamer but absent after cleavage. these features may be rela ... | 1979 | 439892 |
| [reaction between the protein of gene 32 of phage t4 with dna. comparison of the properties of native protein and the product of its limited hydrolysis]. | interaction between dna and limited hydrolysate of t4 gene 32 protein has been studied. this limited hydrolysate is obtained when 8000 dalton segment of native 32 protein is removed proteolitically, its molecular weight being 26,000 dalton. it is shown that the melting temperatures of dna as well as that of the synthetic homopolymer poly[d(at)] complexed with this modified protein are more than 60 degrees lower than that of pure dna and poly[d(at]. the secondary structure defects in dna promote ... | 1979 | 440308 |
| mutator effects of alleles of phage t4 genes 32, 41, 44, and 45 in the presence of an antimutator polymerase. | 1979 | 442526 | |
| expression of bacteriophage t4 genes 45, 44, and 62. i. discoordinate synthesis of the t4 45- and 44-proteins. | 1979 | 442532 | |
| expression of bacteriophage t4 genes 45, 44, and 62. ii. a possible regulatory site between genes 45 and 44. | 1979 | 442533 | |
| effect of long-term gh administration on pituitary-thyroid function in idiopathic hypopituitarism. | twenty-four euthyroid children with idiopathic pituitary dwarfism were studied. the euthyroid state for seven of these patients was determined by negative physical examinations, normal plasma t4 assays and normal 131i uptakes. for the other children, thyroid function was evaluated with t3 and t4 assays and on the basis of the trh test. each of the children was treated with hgh in one of three different ways. the first group (five cases) was given a hgh dose, ranging from 12.4 to 17.2 iu/m2//week ... | 1979 | 443041 |
| misleadingly low free thyroxine index and usefulness of reverse triiodothyronine measurement in nonthyroidal illnesses. | nonthyroidal illness is frequently associated with subnormal serum thyroxine (t4) and free t4 index. to unravel the resultant diagnostic problems, we have studied several variables of thyroid function in the sera of 47 patients hospitalized with nonthyroidal illnesses and seven hypothyroid patients encountered during the same period. of the 47 euthyroid sick patients, 18 had low t4. among these 18, free t4 index was normal in only five, whereas free t4 concentration measured by equilibrium dialy ... | 1979 | 443685 |
| [calcitonin-cells in the thyroid of the human fetus. immunocytochemical study (author's transl)]. | calcitonin (ct)-cells were detected, by using an anti-human calcitonin serum (hct), in the thyroid of the normal and anencephalic human fetuses. the first ct-cells were observed at 14 weeks of gestation. the ct-cells were at first isolated afterwards were appeared in parafollicular localization. the ct-cells were only observed in the middle of the upper or medium third or the lateral lobes, along the central axis of the lobes. no ct-cells were detected in the isthmic region or in the inferior th ... | 1979 | 443736 |
| [quantitative aspects of iodine metabolism in one case of congenital iodotyrosine deiodinase defect (author's transl)]. | in a 27 years old patient an iodotyrosine deiodinase defect was responsible for a profound hypothyroidism (t4-ria: indetectable -- tsh: 190 microu/ml) associated with a large goiter (about 300 g). mit and dit secretions were measured from the urinary cumulative specific activities, and the molar mit/dit ratio was 2.2. the thyroidal iodine exchangeable pool was as low as 177 micrograms. in two comparable patients rendered euthyroid by lipodol injection, total thyroidal 127i pool was around 40 mg ... | 1979 | 443737 |
| [multiple effects of resorcinol on thyroid function (author's transl)]. | in rats, i.v. injected resorcinol led to a decrease in thyroid radioiodine uptake and labelled iodothyronine/iodotyrosine ratio with no changes in the percent of thyroidal labelled iodide or radioiodine release. when thyroid lobes were incubated in vitro with radioiodine, resorcinol concentrations from 10(-3)m to 10(-6)m gave an increase in the percent of thyroidal labelled iodide and iodotyrosine/iodothyronine ratio. in animals fed 5% resorcinol for 2 weeks we observed an increase in thyroid we ... | 1979 | 443741 |
| plasma triiodothyronine concentrations in breast cancer. | plasma triiodothyronine (t3), thyroxine (t4) and thyroid-stimulating hormone (tsh) were measured in 44 early breast cancer patients, 48 with advanced breast cancer, 20 with cancer of the colon, and 56 healthy women of similar age. the plasma t3 concentrations were reduced significantly in both early and advanced breast cancer, but only in those colonic cancer patients with metastatic disease. the mean plasma tsh levels were higher in the breast cancer patients than in the other groups, but the i ... | 1979 | 445341 |
| circulating thyroid hormones in a patient with hyperviscosity syndrome. | the elevated t4 levels by one ria in the patient with hyperviscosity syndrome were probably caused by the interference of the increased protein concentration in the t4 assay rather than by hyperthyroidism or binding of t4 by immunoglobulins. | 1979 | 445846 |
| [the influence of primidone on thyroid function (author's transl)]. | the influence of primidone on thyroxine level, t3 index, ft4 index, triiodothyronine level, t3 (ria)/t4 (ria) quotient as well as on the tsh basal and stimulated values was investigated in 30 children on long-term treatment. the values obtained were compared statistically with those of a normal group. during primidone treatment a drop in t4 level and in ft4 index as well as an increase in t3 (ria)/t4 (ria) quotient was observed. on the other hand the triiodothyronine level and the t3 index were ... | 1979 | 446322 |
| presence and influence of cholinergic nerves in the mouse thyroid. | the presence and influence of cholinergic nerves in the mouse thyroid was studied by histochemistry and measurements of changes in blood radioiodine (bri) levels. numerous nerve fibers displaying specific acetyl choline esterase activity were found, not only as a dense network around vessesl but also as single fibers running around and between thyroid follicles. in stress-adapted normal mice, injection of carbamyl choline (cch) reduced the bri levels. in mice whose tsh secretion was suppressed b ... | 1979 | 446418 |
| depletion of l-3,5,3'-triiodothyronine and l-thyroxine in euthyroid calf serum for use in cell culture studies of the action of thyroid hormone. | gh1 cells are a clonal strain of rat pituitary tumor cells which synthesize gh and prl. we have previously demonstrated that these cells respond to physiological concentrations of l-t3 and l-t4 when cultured with medium supplemented with thyroidectomized calf serum to achieve a thyroid hormone-depleted state under cell culture conditions. in this study, we describe a method to deplete euthyroid calf serum of l-t3 and l-t4 using an anion exchange resin. we demonstrate that the procedure only mini ... | 1979 | 446419 |
| postnatal maturation patterns of serum corticosterone and growth hormone in rats: effect of chronic thyroxine administration. | the effects of chronic neonatal hyperthyroidism in rats on the ontogenic pattern of serum corticosterone and growth hormone (gh) were studied. thyroxine (t4) treated and saline injected rat pups were sacrificed under basal and stress conditions. in comparison to saline control animals, daily t4 administration (0.4 micrograms/gram body weight) produced a sustained elevation in basal corticosterone levels by day 12 and a significant elevation of serum corticosterone in response to stress by day 4. ... | 1979 | 447204 |
| polynucleotide block polymers consisting of a dna.rna hybrid joined to a dna.dna duplex. synthesis and characterization of dgn.rcidck duplexes. | the synthesis of several nucleic acid block polymers of the general type dgn.rcidck is described. the key steps in this procedure were the joining of dck oligomers, protected at the 3'-oh with an acetyl group, to rci oligomers by t4 dna ligase and the purification of the products by rpc-5 column chromatography. the block polymers were characterized by 20% polyacrylamide gel electrophoresis, uv and cd spectra, analytical cs2so4 buoyant density analyses, helix-coil transitions and s1 nuclease stud ... | 1979 | 447659 |
| temperature and compositional dependence of the structure of hydrated dimyristoyl lecithin. | differential scanning calorimetry and x-ray diffraction techniques have been used to investigate the structure and phase behavior of hydrated dimyristoyl lecithin (dml) in the hydration range 7.5 to 60 weight % water and the temperature range -10 to +60 degrees c. four different calorimetric transitions have been observed: t1, a low enthalpy transition (deltah approximately equal to 1 kcal/mol of dml) at 0 degrees c between lamellar phases (l leads to lbeta); t2, the low enthalpy "pretransition" ... | 1979 | 447695 |
| comparative effectiveness of dextrothyroxine and levothyroxine in correcting hypothyroidism and lowering blood lipid levels in hypothyroid patients. | data reported here establish that treatment regimens of 4 mg dextrothyroxine and 0.15 mg levothyroxine in hypothyroid subjects produce similar degrees of lowering of serum tsh, cholesterol, triglycerides, and phospholipid levels and equal stimulation of metabolic rate. the murphy-pattee total t4 determination applied to blood samples drawn 24 h after the last dose of dextrothyroxine can be used to assess adequacy of treatment. correction of hypothyroidism requires high serum levels of dextrothyr ... | 1979 | 447807 |
| inhibition of intrapituitary thyroxine to 3.5.3'-triiodothyronine conversion prevents the acute suppression of thyrotropin release by thyroxine in hypothyroid rats. | iopanoic acid has been shown to block thyroxine (t4)-5'-monodeiodination in rat anterior pituitary in vitro. to test the hypothesis that the acute decrease in thyrotropin (tsh) after infusion of t4 into hypothyroid rats requires intrapituitary t4 to 3,5,3'-triiodothyroxine (t3) conversion, the effect of iopanoic acid treatment on the generation of nuclear t3 from intrapituitary conversion and the response to tsh were compared in control and iopanoic acid-treated animals. 5 mg/100 g body weight i ... | 1979 | 447848 |
| total and free thyroid hormone concentrations after elective surgery. | changes in thyroid hormone concentration and distribution and plasma cortisol levels have been followed in 11 patients undergoing elective cholocystectomy. a significant rise in total and free thyroxine (t4) and fall in total and free triiodothyronine (t3) were noted after surgery. reverse t3 concentrations rose substantially, suggesting that peripheral conversion of t4 to t3 is diminished and that there is preferential formation of reverse t3. serum thyroid stimulating hormone concentrations di ... | 1979 | 447866 |
| studies on the effects of rapeseed meal on thyroid status of cattle, glucosinolate and iodine content of milk and other parameters. | the effects of feeding rapeseed meals (rsm) containing low (tower) or high (target/turret) levels of glucosinolates on thyroid status, iodine and glucosinolate content of milk and other parameters were studied in dairy cows and young calves. rsm (tower and turret) fed to dairy cows at 25% of the grain mixture reduced iodine content of milk. diets containing tower and turret rsm tended to reduce plasma thyroxine (t4) in cows and increase the size of thyroids in rats. calf diets containing target ... | 1979 | 448457 |
| acute suppurative thyroiditis in children. | ten cases of acute suppurative thyroiditis were studied. all patients remained euthyroid clinically during the course of this disease and during the follow-up. of seven cases in which bacterial cultures were performed, an aerobic bacterium alone was isolated in one patient and anaerobic bacteria alone in four patients. mixed aerobic and anaerobic microorganisms were recovered from one patient and cultures were negative in one case. plasma tsh, t4, t3, and pbi levels were normal except in one cas ... | 1979 | 448533 |
| the aging thyroid. increased prevalence of elevated serum thyrotropin levels in the elderly. | of 344 relatively healthy persons older than 60 years, 22 (5.9%) had a clearly elevated level of serum thyrotropin (tsh) (greater than 10 muu/ml), a finding more common in women than in men. ten of the 22 had low values for serum thyroxine (t4) and free t4 (ft4) index, but only one had a low value for serum triiodothyronine (t3) or free t3 (ft3) index. a further 14.4% had a slightly elevated level of serum tsh (greater than 5 less than or equal to 10 muu/ml), but none had low values for serum t4 ... | 1979 | 448912 |
| the diagnostic dilemma of isolated hyperthyroxinemia in acute illness. | eighteen patients with a variety of illnesses but no history of thyroid disease were shown to have elevated serum total and free thyroxine (t4) concentrations but low serum total triiodothyronine (t3) levels. thyroid function tests were requested initially to excluded the possibility of hyperthyroidism in a setting of hypermetabolism. clinical and laboratory follow-up investigations demonstrated that 15 patients were euthyroid and that hormonal alterations were transient features of the intercur ... | 1979 | 448913 |
| changes in extrathyroidal conversion of thyroxine (t4) to 3,3',5-triiodothyronine (t3) in vitro during development and aging of the rat. | changes with age in the rate of in vitro conversion of thyroxine (t4) to triiodothyronine (t3) by organ homogenates were determined in female wistar rats from 6 days to 18 months old. the conversion rates in liver and kidney homogenates of pups before day 13 are very low, and then rise to reach the maximum value at around day 23. during the period of sexual maturation the conversion activity suddenly decreases, and shows afterwards a gradual decline with age. the activity in both liver and kidne ... | 1979 | 449421 |
| [serum levels of t3 and t4 in diabetic and non-diabetic obese subjects]. | 1979 | 450307 | |
| the effect of antibiotics on the t4 polynucleotide ligase catalyzed template dependent polymerization of oligodeoxythymidylates. | the poly(da) dependent t4 polynucleotide ligase catalyzed polymerization of oligodeoxythymidylates is dependent upon duplex stability. the antibiotics ethidium bromide, netropsin and hoechst 33258 stabilize the duplex poly(da) . p(dt)n (n = 6-10) to thermal denaturation. ethidium bromide to dna ratio of 1.25 and netropsin or hoechst 33258 to dna ratio of 0.1 the tm of d(pt) 10 . poly (da) was increased by 10 degrees and 25 degrees c respectively. the t4 polynucleotide ligase activity was not inh ... | 1979 | 450717 |
| [the relationship between haptoglobin type and serum agglutination titers against group g streptococci with the t4-antigen in pregnant women and newborn infants]. | the relationship between haptoglobin type and titer of agglutination against streptococci carrying the t4-antigen was examined in 100 pairs of sera (mother/child). in healthy pregnant women high titers were predominant in individuals of haptoglobin type 2--2. lower titers were found in sera of women with diseases during pregnancy. in 4% of the newborns only the haptoglobin type could be recognized. in comparison of maternal and neonate titers of agglutination pointed out the antibody-like activi ... | 1979 | 452758 |
| gestational choriocarcinoma and thyrotoxicosis. | a case of a 26-year-old woman with metastatic choriocarcinoma and clinical and biochemical thyrotoxicosis is described. this represents the eighth reported case of the association of choriocarcinoma and thyrotoxicosis. serial monitoring of serum thyroxine (t4) and thyroid stimulating hormone (tsh) levels correlated precisely with the beta sub-unit human chorionic gonadotropin (hcg) level and the quantitation of host tumor burden. the development of a hypermetabolic syndrome in patients with chor ... | 1979 | 455255 |
| exchange of triiodothyronine derived from thyroxine with circulating triiodothyronine as studied in the rat. | at present it is widely assumed that t3 derived from t4 is rapidly and totally exchangeable within the volume of distribution of t3 secreted by the thyroid into the bloodstream. this concept is implied when conclusions are drawn from comparisons between a biological effect in a responsive tissue and circulating t3 and t4 levels. such conclusions are often in conflict with those derived by comparing the biological effect with the concentrations of t3 and t4 in the responsive tissue itself. thus, ... | 1979 | 455743 |
| acute effects of hypothalamic ablation on plasma thyrotropin and prolactin concentrations in the suckling rat: evidence that early postnatal pituitary-thyroid regulation is independent of hypothalamic control. | hypothalamic ablation was performed at various periods postnatally in animals previously administered propylthiouracil to raise plasma tsh concentrations. there was no significant change in plasma tsh up to 8 h after hypothalamic ablation in pups 1--4 days old, whereas hypophysectomy of such pups produced a 60% fall in plasma tsh within 4 h. by the 5th postnatal day, hypothalamic ablation produced a 30% fall in plasma tsh within 4 h (p less than 0.05). by the 12th postnatal day and thereafter, t ... | 1979 | 456325 |
| inhibition of the growth of morris hepatoma no. 44 in rats after induction of hypothyroidism: evidence that morris hepatomas are thyroid dependent. | the growth rate of morris hepatoma no. 44 (generation time, 6 mo) was inhibited after the induction of hypothyroidism by propylthiouracil (ptu) (0.1% in purina chow), i131 (1 mci/100 g body wt i.p.), or surgical thyroidectomy. after 11 wk of treatment, hepatoma weight was 66%, 87%, and 75% (after correction for total body wt) relative to controls in ptu-fed, i131 injected, and thyroidectomized rats, respectively. in each case, exogenous thyroxine (t4) (8 microgram/kg body wt i.p.) reversed these ... | 1979 | 456848 |
| changes in serum concentrations of 3,3',5'-triiodothyronine and 3,5,3'-triiodothyronine during prolonged moderate exercise. | the effect of moderate bicycle exercise (3.5 h) on peripheral thyroid hormone metabolism was studied under two conditions (with and without glucose infusion) in four normal males. serum t3, rt3, total protein, plasma glucose, and ffa were determined. exercise induced an increase in rt3 from 29 to 40 ng/dl (p less than 0.01), a decrease in t3 from 154 to 147 ng/dl (p less than 0.01), and an increase in t4 from 7.1 to 7.5 micrograms/dl (p less than 0.05). when glucose was infused during exercise, ... | 1979 | 457843 |
| [diagnostic procedures in diencephalo-hypophyseal insufficiency (author's transl)]. | a functional diagnosis of the diencephalohypophyseal system was carried out in patients with sheehan syndrome, chromophobic adenoma, craniopharyngioma, prolactin-producing pituitary tumours, acromegaly, hypothalamo-pituitary dwarfism and constitutional retardation. a combined insulin hypoglycaemia/lh-rh/tsh test was performed to define frequency and extent of anterior pituitary insufficiency. with these illnesses, almost generally, a somatotropic insufficiency (except in acromegaly) was found. ... | 1979 | 459366 |
| non-thyrotropic pituitary cells in culture convert t4 to t3. | 1979 | 459693 | |
| [normal values of t3-index and of thyroxine (t4) (author's transl)]. | the normal values of t3-index and of thyroxine (t4) have been evaluated comparing a binomial distribution to frequential curve of all the values obtained. the findings are: t3-index = 89 divided by 112; t4 = 3.7 divided by 15.3 microgram/100 cm3 of serum. | 1979 | 461849 |
| serum endogenous thyroxine (t4) and plasma thyroid stimulating hormone (tsh) levels during anaesthesia and surface surgery in children--a preliminary report. | 1979 | 463338 | |
| active and inactive thyroid hormone levels in elective and acute surgery. | the changes in the plasma or serum concentration of thyrotrophin (tsh), thyroxine (t4), 3,5,3'-triiodothyronine (t3), 3,3',5-triiodothyronine (reverse t3, rt3) and cortisol were examined in patients undergoing elective cholecystectomy (n = 10) or acute laparotomy due to peritonitis (n = 11). tsh and t4 showed no essential changes in either group. t3 was reduced already during initiation of anaesthesia and continued to fall during and after surgery in both groups. in the peritonitis group, t3 was ... | 1979 | 463442 |
| serum triiodothyronine and other clinical and laboratory indices of alcoholic liver disease. | admission serum triiodothyronine (t3) values in 124 patients hospitalized for alcoholic liver disease were correlated with clinical and laboratory indices of liver function and commonly used determinants of thyroid function. patients with low admission serum t3 levels had significant alterations in serum albumin, bilirubin, prothrombin time, and alkaline phosphatase associated with clinical signs of portal hypertension and collateral circulation, with little difference in serum glutamic-oxaloace ... | 1979 | 464436 |
| thermal analysis of bacteriophage t4. | 1979 | 465046 | |
| effect of allyl isothiocyanate on plasma and urinary concentrations of some biochemical entities in the rat. | allyl isothiocyanate (aitc) is a constituent of several plants of the family cruciferae that are commonly used as food. this study investigated the effect of feeding aitc to male sprague-dawley rats on their plasma glucose and uric acid levels as well as on the urinary concentrations of glucose, 17-ketosteroids (17-ks), creatinine, and uric acid. other test compounds included were thyroxine (t4) and thiouracil (tu). aitc caused a highly significant (p smaller than or equal to 0.01) depression in ... | 1979 | 466577 |
| [fundamental and clinical studies on solid-phase radioassay kits "spac t3 uptake and t4 ria" (author's transl)]. | 1979 | 466833 | |
| the hypothalamic-pituitary-gonadal axis during hyperthyroidism in the rat. | the rat, an animal without testosterone-estrogen-binding-globulin, was treated with l-t4 to the point of hyperthyroidism in order to study the hypothalamic-pituitary-testicular axis during this condition. hyperthyroidism led to a significant decline in serum fsh, a fall in serum lh which was not satistically significant, and no change in serum levels of testosterone or estradiol. testes of hyperthyroid rats produced significantly more testosterone during in vitro incubations than did the testes ... | 1979 | 467326 |
| commentary: the cut-off point for tsh measurement or recalls in a screening program for congenital hypothyroidism using primary t4 screening. | 1979 | 469665 | |
| crystalline aggregation of a proteolytic fragment of the major head protein of bacteriophage t4. | 1979 | 469944 | |
| [immunological and sedimentation analysis of uv-irradiated t4 phage dna containing 5-bromouracil]. | conversions resulting from uv-irradiation of 5-bromouracil dna of t4 phage were studied. the use of immunological and sedimentation techniques revealed that uv dose ranging from 1.4.10(2) to 1.4.10(3) erg/mm2 induced unwinding and single-stranded breaks in 5-bromouracil dna which are not accompanied by debromination. beginning with 4.10(3) erg/mm2 debromination but not degradation of dna is detected. doses higher than 4.10(3) erg/mm2 lead to extensive degradation of 5-bromouracil t4 dna. | 1979 | 470946 |
| [effects of venous compression for blood sampling on the results of in vitro tests of thyroid function (author's transl)]. | in a group of subjects with a normal as well as pathological thyroid function there was a significant increase in rt3u, serum-thyroxine-concentrations and tsh-levels but there were no significant changes in the t4-rt3u-indices and etr-tests after 5 min of venous compression. the discordant results were obtained in 21 cases (26.9%) with the rt3u-test and in 4 cases (5,1%) with the t4-serum-level-determination, which may cause diagnostic errors. the non-standardized venous compression time causes ... | 1979 | 471777 |
| t4 gene 40 mutants. i. isolation of new mutants. | 1979 | 473595 | |
| t4 gene 40 mutants. ii. phenotypic properties. | 1979 | 473596 | |
| effect of iodine intake and food consumption. | rats were exposed to cold (4 degrees c) for 1 and 4 weeks. the t4 plasma concentrations initially declined (24.5 +/- 7.7 nmol/l) after 1 week but returned to normal levels after 4 weeks (52.9 +/- 14.2 nmol/l). the t3 concentrations were elevated after both 1 and 4 weeks at 4 degrees c (1.31 +/- 0.21 and 1.38 +/- 0.12 nmol/l, respectively). control values (23 degrees c) for t4 were 42.6 +/- 10.3 and for t3 1.11 +/- 0.13 nmol/l. addition of 0.015 g ki/l to the drinking water prevented the t4 decre ... | 1979 | 474039 |
| studies on the origin of altered thyroid hormone levels in the blood of rats during cold exposure. ii. effect of propranolol and chemical sympathectomy. | the effect of sympathetic activity on t4 and t3 levels in cold-exposed rats was investigated. administration of the highest dose of propranolol (2 mg/100 g b.w.) twice daily during 4 days decreased t4 and t3 concentrations in plasma of rats living at 23 degrees c (t4 from 46.4 +/- 2.6 to 25.8 +/- 5.3 nmol/l and t3 from 1.08 +/- 0.6 to 0.82 +/- 0.12 nmol/l). no significant effect on t4 and t3 levels (49.0 +/- 11.6 and 1.48 +/- 0.16 n/mol, respectively) after the administration of the same dose re ... | 1979 | 474040 |
| changes in pituitary and thyroid function with increasing age in young male rats. | as the age of young adult male rats increased from 30 to 150 days, the serum thyroxine (t4) decreased by 50% and the serum thyroid-stimulating hormone (tsh) increased by 250%. there was no change in the serum triiodothyronine (t3). the increment in serum tsh after injection of thyrotropin-releasing hormone (trh) was not significantly different at any of the ages studied, but the old animals had significantly lower increments in serum t4 and t3 after subcutaneous administration of bovine tsh. des ... | 1979 | 474748 |
| effect of winter sleep on pituitary-thyroid axis in american black bear. | during winter sleep the black bear has decreased levels of serum total and free thyroxine (t4) and triiodothyronine (t3) and a prolonged, delayed response of serum thyrotropin (tsh) (bioassay) to thyrotropin-releasing hormone (trh). four weeks after the end of winter sleep, levels of serum thyroid hormones increase, and tsh response to trh is short and brisk. serum t4 and t3 rise after trh administration both during and after winter sleep; however, the maximum increment in serum t3 is greater du ... | 1979 | 474749 |
| complementary transcribed t4 rna. association with the polysomes. | 1979 | 475778 | |
| rare transfer ribonucleic acid essential for phage growth. nucleotide sequence comparison of normal and mutant t4 isoleucine-accepting transfer ribonucleic acid. | one of the eight trna species coded by bacteriophage t4 is unique in that (1) it is found in a yield lower by three- to fourfold than that of any other trna and (2) while dispensable for growth in standard laboratory hosts, it is essential for phage propagation in a natural isolate of escherichia coli (strain ct439). we report here the nucleotide sequence of this trna and of several mutationally altered forms. the molecule is 77 nucleotides in length and has the anticodon n-a-u. depending on the ... | 1979 | 476088 |
| plasma thyroid hormones and cholesterol in the newborn of genetically different types of cattle in a tropical environment. | the patterns of plasma concentrations of thyroxine (t4), triiodothyronine (t3) and cholesterol were determined in tropically adapted (zx) and temperate (sh) breeds of cattle in the first 6 days of their lives in a tropical environment. t4 concentrations were high at birth and fell to within normal adult values at 6 days of age. in contrast, plasma cholesterol levels were low at birth and increased daily to the highest values on day 6. t3 levels increased from birth to maximum values at 12 h afte ... | 1979 | 476214 |
| amniotic fluid concentrations of 3,3',5'-tri-iodothyronine (reverse t3), 3,3'-di-iodothyronine, 3,5,3'-tri-iodothyronine (t3) and thyroxine (t4) in normal and complicated pregnancy. | amniotic fluid concentrations of 3,3',5'-tri-iodothyronine (rt3), 3,3'-di-iodothyronine (3,3'-t2), 3,5,3'-tri-iodothyronine (t3) and t4 were studied in 384 women during normal and complicated pregnancy. an inverse correlation was observed between decreasing rt3 and increasing 3,3'-t2 concentrations in amniotic fluid with gestational age. the mean rt3 level in normal pregnancy was 2.81 nmol/1 at 12-20 weeks and decreased significantly to 1.06 nmol/1 at 36-42 weeks of gestation. the mean 3,3'-t2 c ... | 1979 | 476970 |
| disturbed mineral metabolism in hyperthyroidism: good correlation with tri-iodothyronine. | mineral metabolism is frequently disturbed in hyperthyroidism. in a group of seventy-two patients with hyperthyroidism, we observed an increase in serum diffusible calcium in 50% of the cases, elevated inorganic phosphorus in 30% and elevated alkaline phosphatase in 44% of the cases. correlations existed between the values of diffusible calcium, inorganic phosphorus, alkaline phosphatase and certain indices of thyroid function (t4, ft41, t3, ft3i), of which that with t3 was the best (p less than ... | 1979 | 476975 |
| serum t3 and t4 levels in patients with anorexia nervosa showing transient hyperthyroidism during weight gain. | serum thyroxine (t4) and triiodothyronine (t3) levels were measured in a group of thirty-three patients with anorexia nervosa (an) and compared with twenty-five control women presenting with hirsutism and twenty-one patients with primary myxoedema. t3 levels in the an patients were significantly lower than in the control subjects and in the patients with myxoedema while t4 levels were significantly higher than in the patients with hypothyroidism but significantly lower than in the control group. ... | 1979 | 476976 |
| screening for neonatal hypothyroidism by thyroxine and thyrotrophin radioimmunoassays using dried blood samples on filter paper. | a routine and automatized methodology for thyroxine (t4) and thyrotrophin (tsh) radioimmunoassay (ria) using dried blood samples on filter paper is described. t4-ria was performed on one single dot (5 mm diameter equivalent to 4 microliters of serum) while two dots were necessary for tsh-ria. reference filter papers were introduced in each assay for quality control. in a preliminary study on 4,155 neonates, samples generally obtained between the 5th--7th day gave a mean 'dot-t4' of 97.95 +/- 36. ... | 1979 | 476983 |
| [functional role of the basal lamina proteins of bacteriophage t4]. | 1979 | 477508 | |
| [effect of a 2 mg thyroxine bolus on the hypophyseal-thyroid axis (author's transl)]. | the effect of a single dose of 2 mg l-thyroxine on central and peripheral parameters of the hypophyseal-thyroid axis was studied on ten euthyroid and ten hypothyroid patients, and ten with euthyroid goitre. without the occurrence of hyperthyroid symptoms, the t4 serum level was raised for five days in the euthyroid group, for 11 days in the hypothyroid ones. during this time the t3 serum level did not alter. suppression of iodine uptake by the thyroid and tsh secretion of the hypophysis persiste ... | 1979 | 477562 |
| propranolol inhibits the in vitro conversion of thyroxine into triiodothyronine by isolated rat liver parenchymal cells. | a model for the in vitro study of the conversion of thyroxine into triiodothyronine using isolated rat liver parenchymal cells is described. isolated liver cells (mean protein content 18 mg/ml) convert approximately 0.8% of 1.3 microm exogenously added t4 into t3 during thirty minutes incubation. carbimazole (50 microm) has no effect on the conversion process, whereas propylthiouracil (50 microm) inhibits the conversion. the beta-adrenoceptor blocking agent propranolol lowers the conversion rati ... | 1979 | 478432 |
| the role of thyronine in thyroid hormone metabolism. | thryonine (t0) has been identified in human urine using gas chromatography-mass fragmentography (g.c.m.f.). in 22 normal individuals urinary t0 concentration was found to range between 8--25 nmol/24h. assuming the mean normal thyroxine (t4) production rate to be approximately 100 nmol/24h, our findings indicate that less than 20% of this could be accounted for as urinary t0 excretion, thus supporting earlier findings that the peripheral metabolism of t4 is not limited solely to deiodination. | 1979 | 479355 |
| stimulation of recombination in phage t4 by nitrous acid-induced lesions. | recombination of phage t4 can be stimulated more than sixfold above the spontaneous level by treatment with nitrous acid, indicating that the lesions induced by this agent are strongly recombinogenic. two temperature sensitive mutants defective in exonuclease functions showed less than the wild-type spontaneous level of recombination even after nitrous acid treatment and a ligase mutant showed a highly elevated frequency of recombination after treatment. since these same mutants have analogous e ... | 1979 | 479852 |
| gene 24-controlled osmotic shock resistance in bacteriophage t4: probable multiple gene functions. | by use of mixed infections with conditional lethal mutations in the head genes and an osmotic shock-resistant mutant we have demonstrated that osmotic shock resistance is controlled by gene 24. using acrylamide gel electrophoresis combined with the "immune replicate" technique, we confirmed the positions of gene products 24 and 24* (p24 and p24*). in this paper we have still used the notation "p24," etc., for designating the product of gene 23, etc., although we prefer and use in general the des ... | 1979 | 480456 |