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studies of nondefective adenovirus 2-simian virus 40 hybrid viruses. v. isolation of additional hybrids which differ in their simian virus 40-specific biological properties.four new nondefective adenovirus 2 (ad2)-simian virus 40 (sv40) hybrid viruses have been isolated. although these viruses (designated ad2(+)nd(2), ad2(+)nd(3), ad2(+)nd(4), and ad2(+)nd(5)) were clonal derivatives of the same ad2-sv40 hybrid population, they differ significantly from each other and from the previously isolated nondefective hybrid, ad2(+)nd(1), in their biological properties or in the amount of sv40-specific rna induced during lytic infection.like ad2(+)nd(1), ad2(+)nd(2), and ad ...19734350710
breeding the african green monkey, cercopithecus aethiops, in a laboratory environment. 19734351470
relationship of replication and transcription of simian virus 40 dna.rna produced by the simian virus 40 (sv40) mutant tsa30 during lytic infection of kidney cells of african green monkeys was examined by rna-dna competition-hybridization. this mutant is temperature-sensitive in a function (gene a) that regulates synthesis of viral dna. no detectable difference between mutant rna synthesized at the permissive temperature (33 degrees ) and wild-type viral rna was found. during continuous infection with the mutant at the restrictive temperature (41 degrees ) only e ...19734352963
characteristic behaviour of the cocultures of the sv40 transformed rabbit kidney cells and african green monkey kidney cells. 19724353413
the fragmentation of cellular dna and the formation of pseudovirions during sv 40 infection of african green monkey kidney cells. 19734355330
protein kinase stimulated by cyclic gmp in uninfected and simian virus 40-infected monkey kidney cells.uninfected african green monkey kidney cells contain a cyclic gmp-stimulated protein kinase. the level or specific activity of the enzyme was not significantly increased after infection of the cells with simian virus 40. this enzyme probably catalyzes the phosphorylation of the structural proteins of the virus.19744359424
susceptibility of the vero line of african green monkey kidney cells to human enteroviruses.the relative susceptibility of vero cells and primary rhesus monkey kidney cells to 47 prototype strains of human enteroviruses is described. of these strains, types 4, 14, 16, 17, 18, 21, 31 and 34 and coxsackie virus a 9 failed to cause cpe in the vero cells whilst only one, echovirus type 34, failed to cause cpe in the monkey kidney cells.a comparison is given of the efficiency of the two cell cultures for enterovirus isolation from clinical material. results show that vero cells are as usefu ...19744361500
maintenance of primary african green monkey kidney (pagmk) and vero cells at room temperature (25 degrees). a system for virus isolation in community practice. 19744362601
experimental herpesvirus infection of baboons (papio cynocephalus) and african green monkeys (cercopithecus aethiops) and recovery of virus by tissue explants. 19734362931
synthesis of simian virus 40 dna in isolated nuclei.nuclei isolated from african green monkey kidney cells infected with simian virus 40 at different times after infection maintain in vitro the same temporal sequence of host and viral dna synthesis as that seen in intact cells. the viral dna synthesized by the nuclei of cells previously infected for 32-35 hr was characterized by centrifugation through neutral and alkaline sucrose gradients, and by isopycnic banding in a propidium iodide-cesium chloride gradient. dna synthesis in this system is ma ...19744364525
conditional lethal mutants of adenovirus 2-simian virus 40 hybrids. i. host range mutants of ad2+nd1.human adenovirus type 2 (ad2) grows poorly in monkey cells, although this defect can be overcome by co-infection with simian virus 40 (sv40). the nondefective ad2-sv40 hybrid virus, ad2(+)nd1, replicates efficiently in both human and african green monkey kidney cells, presumably due to the insertion of sv40 sequences into the ad2 dna. several mutants of ad2(+)nd1 have been isolated that grow and plaque poorly in monkey cells, although they retain the ability to replicate and plaque efficiently i ...19744364898
biological properties of two strains of simian virus 40 isolated from patients with progressive multifocal leukoencephalopathy.biological properties of two strains of simian virus 40 (sv40) from brains of two patients with progressive multifocal leukoencephalopathy (pml) have been compared to those of a standard laboratory strain of sv40. infectivity of both sv40-pml viruses was resistant to treatment with chloroform, low ph, and 50 c for 120 min. african green monkey kidney and bsc-1 cells were the most sensitive for viral replication, and cytopathology in these cultures was indistinguishable from that caused by sv40. ...19744367120
dna synthesis in resting african green monkey kidney cells infected with defective sv 40. 19744372297
chromosome assignment of the t-antigen gene of simian virus 40 in african green monkey cells transformed by adeno 7-sv40 hybrid.somatic cell hybrids between mouse cells deficient in thymidine kinase [atp:thymidine 5'-phosphotransferase (ec 2.7.1.75)] and two different monkey cell lines transformed by an adeno 7-sv40 hybrid have been produced using both a semiselective and a double selective procedure. concordant segregation of the expression of sv40 t antigen with a specific monkey chromosome has been observed in all the mouse-monkey hybrid clones examined. subcloning of three sv40 t antigen positive hybrid clones result ...19744372607
herpesvirus (strain 0430) lesions in cercopithecus aethiops. 19744375197
adenovirus hemagglutination of african green monkey erythrocytes. 19664380468
on the theory of clonal selection in carcinogenic transformation. 19744469197
the vervet monkey (cercopithecus aethiops) as an experimental model for pulpal studies. 19734513208
experimental schistosoma haematobium infection in vervet monkeys (ceropithecus aethiops centralis). 19724623245
experimental schistosoma mansoni infections in vervet monkeys (cercopithecus aethiops centralis). 19724623246
red cell enzymes and serum proteins of cercopithecus aethiops (south african green monkey). 19724624014
the st. kitts green monkey (cercopithecus aethiops sabaeus): ecology, population dynamics, and selected behavioral traits. 19724624017
comparative immunogenicities of chikungunya vaccines propagated in monkey kidney monolayers and chick embryo suspension cultures.a comparative study was made of formalin-inactivated chikungunya vaccines prepared from the virus propagated in african green monkey kidney monolayers and concentrated chick embryo suspension cultures. the vaccine prepared in the chick embryo suspension cultures was significantly more protective to mice against a live homologous virus challenge and stimulated the production of 4 to 5 times more circulating antibodies than the vaccine prepared with virus grown in african green monkey kidney monol ...19724624213
chromatin structure and the cell cycle.pancreatic dnase i is used to probe the structure of chromatin isolated from synchronized hela cells. the degree to which dna in chromatin is protected from dnase attack varies during the g(1), s, and g(2) phases of the cell cycle. in addition, the dnase sensitivity of chromatin from contact-inhibited african green monkey kidney cells differs from that of actively dividing, subconfluent cultures. these cell cycle-dependent chromatin changes were observed consistently at all enzyme concentrations ...19724626402
comparative features of the reproductive biology of hamadryas baboons (papio hamadryas), grivet monkeys (cercopithecus aethiops) and rhesus monkeys (macaca mulatta). 19724627306
dna replication in synchronized cultured mammalian cells. iv. replication of african green monkey component and bulk dna. 19724627587
replication of avian infectious bronchitis virus in african green monkey kidney cell line vero. 19724627930
rabies antibody determination by immunofluorescence in tissue culture.a tissue culture (tc)-fluorescent antibody (fa) technique for the measurement of rabies-neutralizing antibody was found to be reliable and comparable to the standard mouse serum neutralization test. this test was performed with bhk-21 cells infected with the era vaccine virus strain on lab-tek tc chamber slides. a flury high egg passage (hep) rabies virus strain grown on continuous line of african green monkey (vero) and on bhk-21 cells was investigated to determine its utilization in a tc-fa ne ...19724628958
a blood-aqueous barrier to small molecules in the ciliary processes of the vervet monkey (cercopithecus aethiops). 19724630657
survival of animal tissue cells in primary culture in the absence of serum.the ability of cells from tissues of several species of animals to survive in primary culture without serum was tested. of the species tested, cells from the kidneys of macaca mulatta (rhesus) and cercopithecus aethiops (vervet) monkeys and chicken embryo cells not only survived under these conditions, but indeed developed into confluent monolayer cultures. the addition of either serum or its globulin or albumin fraction enhanced the development of cell monolayers and permitted those cells unabl ...19734631437
vitamin c requirements of the vervet monkey (cercopithecus aethiops) under experimental conditions. 19734633567
[some characteristics of ovarian structure and the level of urinary sex steroid excretion in the green marmoset vervet (cercopithecus aethiops pygerythrus)]. 19734634208
cell fusion by canine distemper virus-infected cells.av3 cells (continuous human amnion) infected with the onderstepoort strain of canine distemper virus produced cell fusion within 2 to 5 hr when added to av3 cell monolayers. an apparent requirement for intact, infected cells was demonstrated by showing that (i) frozen-and-thawed infected cells failed to induce fusion, (ii) infected cells frozen in the presence of glycerol retained their ability to induce fusion, (iii) infected cells subjected to swelling in hypotonic buffer and homogenization lo ...19724644630
evolutionary conservatism in arrangement of genetic material. a comparative analysis of chromosome banding between the rhesus macaque (2n equals 42, 84 arms) and the african green monkey (2n equals 60, 120 arms). 19734783711
isolation and propagation of adenoviruses in african green monkey kidney cultures. 19654954392
observations on the menstrual cycle of the grivet monkey. (cercopithecus aethiops aethiops) in the sudan. 19664955692
response of cercopithecus aethiops to cholera vibrio lipopolysaccharide and psychological stress. 19664959386
experimental schistosomiasis in primates in tanzania. i. a preliminary note on the susceptibility of cercopithecus aethiops centralis to infection with schistosoma haematobium and schistosoma mansoni. 19664960090
cytopathic and plaque assay of rubella virus in a line of african green monkey kiency cells (vero). 19674961492
replication of rubella virus in a continuous line of african green monkey kidney cells (vero). 19674961494
social structure among vervet monkeys (cercopithecus aethiops). 19674965299
isolation and characterization of simian adenoviruses isolated in association with an outbreak of pneumoenteritis in vervet monkeys (cercopithecus aethiops). 19674965334
[on an infectious disease transmitted by cercopithecus aethiops. ("green monkey disease")]. 19684966281
[on the neuropathology of the green monkey (cercopithecus aethiops) transmitted infectious diseases in marburg]. 19684966284
a metrical study on the bony palate of macaca irus and cercopithecus aethiops. 19674966387
observations on the fine structure of spermatozoa of the bush baby (galago senegalensis), the african green monkey (cercopithecus aethiops) and man. 19674966398
the relation between the ciliary muscle and the trabecular meshwork and its importance for the effect of miotics on aqueous outflow resistance. a study in two contrasting monkey species, macaca irus and cercopithecus aethiops. 19674967268
problems in the detection of rubella virus in african green monkey kidney tissue culture. 19684968540
rubella hemagglutinin prepared in a serial line african green monkey kidney cells. 19684969859
blood cells in the african green monkey. 19684970328
[studies on the animal-oriented pathogenicity of a cercopithecus aethiops transmitted human pathogenic agent]. 19684971554
[experimental infection of cercopithecus aethiops with the agent of the frankfut-marburg syndrome (fms)]. 19684973288
an infectious disease transmitted by cercopithecus aethiops. ("green monkey disease"). 19684974180
distribution of cholinesterase in the basal rhinencephalic structures of the grivet monkey (cercopithecus aethiops aethiops). 19684975689
[adaptation and selection of a highly attenuated variant of measles virus in primary kidney cultures of cercopithecus aethiops]. 19674976084
production of kidney tissue cultures from african green monkeys, experimentally infected with the causative agent of frankfurt-marburg-syndrome. 19694977816
external morphology of the brain of the givet monkey (cercopithecus aethiops aethiops). 19684978304
comparative sensitivity of tissue cultures to rubella virus: use of guinea pig cells for virus titration.a series of 19 different primary and serial tissue cultures were investigated for their sensitivity to virulent or attenuated rubella virus (rv). primary guinea pig tissues, a serial passage of baby hamster kidney, and primary human amnion were comparable to african green monkey kidney tissue cultures in their sensitivity. in general, primary human tissues were relatively insusceptible to the gilchrist strain of rv. rv interfered with the growth of vesicular stomatitis virus. based on this findi ...19694979943
[the sleep electroencephalogram in cercopithecinae: erythrocerbus patas and cercopithecus aethiops sabaeus]. 19694980057
two classes of spermatogonial stem cells in the monkey (cercopithecus aethiops). 19694982042
biological characteristics and viral susceptibility of an african green monkey kidney cell line (vero). 19694982209
[steroid metabolism in primates. iv. isolation of aldosterone from adrenal venous blood of macacus rhesus, papio hamadryas, cercopithecus aethiops and erythrocebus patas]. 19694982968
sparganosis and "proliferative" spargana in vervets (cercopithecus aethiops) and baboons (papio sp.) from east africa. 19704984083
[electron microscopical findings in a communicable disease in monkeys (cercopithecus aethiops)]. 19684985421
amygdalectomy in the free-ranging vervet (cercopithecus aethiops). 19704985911
[the influence of methoxychlor on tissue culture of the kidney of the monkey cercopithecus aethiops and of the lung of the human embryo]. 19704987182
salivary amylase. ii. alpha-amylase in salivary glands of the macaca irus monkey, the cercopithecus aethiops monkey, and man. 19704988027
contamination of simian viruses in african green monkeys (cercopithecus aethiops). 19694992619
studies of rubella virus. 1. a study of growh of rubella viru in kidney culture cells of african green monkey. 19694993211
characteristics of the bgm line of cells from african green monkey kidney. brief report. 19704993582
an epithelial blood-aqueous barrier to horseradish peroxidase in the ciliary processes of the vervet monkey (cercopithecus aethiops). 19714994081
dna strand reassociation and polyribonucleotide binding in the african green monkey, cercopithecus aethiops. 19714995480
[preliminary observations on the articular receptors of cercopithecus aethiops]. 19714999858
an electron microscopic study of the permeability of iris capillaries to horseradish peroxidase in the vervet monkey (cercopithecus aethiops). 19715000137
subhuman primate diploid cells: possible substrates for production of virus vaccines.results of a program for development of new cell lines suggest that it it possible to establish cell lines from both rhesus and african green monkeys which are comparable to diploid lines of human origin, and that these monkey lines should be candidates for use in the production of virus vaccines.19715000950
play-mothering: the relations between juvenile females and young infants among free-ranging vervet monkeys (cercopithecus aethiops). 19715001014
proposal for classifying human strain navel and related simian mycoplasmas as mycoplasma primatum sp. n.mycoplasmas isolated from simian (cercopithecus aethiops) tissues were shown to have biological, biochemical, and serological properties and electrophoretic cell protein patterns similar to strain navel isolated from man 15 years ago. the simian and human navel strains comprised a single serogroup, distinct from the established mycoplasma and acholeplasma species of the class mollicutes. it is proposed that strains with the properties described be named mycoplasma primatum.19715001200
heterochromatin location on chromosomes of normal and transformed cells from african green monkey (cercopithecus aethiops). dna denaturation-renaturation method. 19715001376
[cultivability in vitro of renal cells of cercopithecus aethiops. attempted obtainment of cell lines]. 19715001764
cultivability in vitro of cercopithecus aethiops renal cells. attempts at obtaining cell lines. 19715005007
paracrystal formation in cell cultures infected with adenovirus type 2.large rod-shaped structures corresponding to paracrystals were seen in the nucleus, cytoplasm, or both of adenovirus type 2 (ad2)-infected cells by immunofluorescence staining with antibody prepared against purified ad2. in exception to this, ad2-induced crystals did not stain with either hexon or fiber antibody. the crystalline structures were first observed in ad2-infected vero cells at 28 hr with a maximum number at 70 hr postinoculation. the kinetics of paracrystalline formation closely para ...19715006035
uptake of heterologous genome by mammalian spermatozoa and its transfer to ova through fertilization.simian virus 40 (sv40) adsorbs on rabbit spermatozoa but does not penetrate the cells, as indicated by the absence of radioactive material seen on autoradiography of spermatozoa exposed to [(3)h]thymidine-labeled sv40. in contrast, after exposure of spermatozoa to labeled sv40 dna, radioactive material was found in the postacrosomal area of the spermatozoa. furthermore, when spermatozoa exposed to sv40 dna were fused with cells of the cv-1 line of african green monkey kidney cells, infectious sv ...19715277085
the neuropathology of an infectious disease transmitted by african green monkeys (cercopithecus aethiops). 19695389368
effect of ultraviolet irradiation on the survival of simian virus 40 functions in human and mouse cells.the relative sensitivities to ultraviolet light of various simian virus 40 (sv40) functions were studied in human and mouse cells. transformation appeared to be less ultraviolet (uv)-sensitive than either t or v antigen when all functions were compared in the same cell. however, the time course of both t- and v-antigen appearance was delayed with uv-irradiated virus, so that the survival curves of these functions changed with time. mouse and human cells which were transformed by uv-sv40 all cont ...19705497884
preliminary communication on gross pathological lesions in the genito-urinary systems of vervet monkeys (cercopithecus aethiops) experimentally infected with schistosoma haematobium. 19705500113
deoxyribonucleic acid replication in simian virus 40-infected cells. iv. two different requirements for protein synthesis during simian virus 40 deoxyribonucleic acid replication.the replication of simian virus 40 (sv40) deoxyribonucleic acid (dna) was inhibited by 99% 2 hr after the addition of cycloheximide to sv40-infected primary african green monkey kidney cells. the levels of 25s (replicating) and 21s (mature) sv40 dna synthesized after cycloheximide treatment were always lower than those observed in an infected untreated control culture. this is consistent with a requirement for a protein(s) or for protein synthesis at the initiation step in sv40 dna replication. ...19715543424
superhelix density heterogeneity of intracellular simian virus 40 deoxyribonucleic acid.covalently closed intracellular and viral simian virus 40 (sv40) deoxyribonucleic acid (dna) were separately isolated from infected african green monkey cells (bsc-1) grown in culture. the two dna species form overlapping bands centered at different positions in a propidium di-iodide-cesium chloride (pdi-cscl) buoyant density gradient capable of separating closed dna species with different superhelix densities. when the dense side of a (32)p-labeled intracellular dna band was mixed with the ligh ...19715543431
a zoonosis associated with african green monkeys. 19685692925
changes in blood glucose levels in african green monkeys following administration of sodium pentobarbital. 19685718755
[an infectious disease transmitted by cercopithecus aethiops ("marbury disease") with glial nodule encephalitis]. 19685748997
characterization of the tacaribe group of arboviruses. i. propagation and plaque assay of tacaribe virus in a line of african green monkey kidney cells (vero). 19676027511
characterization of african green monkey b-cell lines releasing an adult t-cell leukemia-virus-related agent.eight lymphoblastoid cell lines were established from the peripheral blood of individual african green monkeys (agm). the agm-2206 line grew out spontaneously. the others - agm-6, 7, 8, 10, 12, 13, and 16 - were obtained after infection of peripheral agm lymphocytes with cell-free culture supernatant of agm-2206. all lines contained, and were probably transformed by, agm-ebv. moreover, they expressed immunoglobulins but lacked the leu l t-cell marker. thus they were b cells. since a high percent ...19846086534
primary isolation and serial passage of hepatitis a virus strains in primate cell cultures.although several primate cell types have been reported to support replication of hepatitis a virus, optimal conditions for the isolation and production of quantities of virus have not been defined. we therefore examined seven different primate cell types for their ability to support replication of primate-passaged and wild-type virus as reflected by intracytoplasmic accumulation of viral antigen (direct immunofluorescence and radioimmunoassay) and propagation of cell culture-adapted virus. of th ...19846086708
rapid differentiation of herpes simplex virus types 1 and 2 by cytopathic effect in bs-c-1 cells.conditions are defined under which herpes simplex virus (hsv) type 2 produces a characteristic cytopathic effect (c.p.e.) in african green monkey kidney (bs-c-1) cells. the bs-c-1 microplate c.p.e. test was simultaneously evaluated against immunological and biological marker procedures on 359 hsv strains. a bs-c-1 cell suspension was added to serial dilutions of hsv strains in falcon micro test ii plates and incubated at 36.5 degrees c in a 4% co2 atmosphere. when the infected cultures were exam ...19846086814
interruption of a human nuclear sequence homologous to mitochondrial dna by a member of the kpni 1.8 kb family.we reported that several dna sequences homologous to mitochondrial dna (mtdna) are present in the human nuclear genome (tsuzuki et al. (1983) gene 25, 223-229). detailed southern blot analyses revealed that one of such sequences is interrupted by a repetitive sequence about 1.8 kb long, and that the insert is one member of the dispersed repeated dna sequences of the kpni 1.8 kb family. nucleotide sequence analysis showed that the kpni 1.8 kb dna is flanked with imperfect 15-base pair (bp) direct ...19846087277
transmission and scanning electron microscopy of experimental pulmonary simian varicella (delta herpesvirus) infection in african green monkeys (cercopithecus aethiops).the lungs of african green monkeys infected with simian varicella virus (delta herpesvirus) were examined by scanning and transmission electron microscopy. endothelial cell shrinkage, oedema formation and necrosis characterized the scanning electron microscopy of the affected vasculature. oedema of the interalveolar septa with swelling of the capillary endothelial cells was observed by transmission electron microscopy. numerous viral particles were associated with a generalized area of necrosis ...19846088596
nucleosome arrangement in alpha-satellite chromatin of african green monkey cells.by analyzing the accessibility of restriction endonuclease sites in african green monkey alpha-satellite chromatin, we demonstrate the absence of a unique phase relationship between nucleosomes and alpha-satellite dna. the data indicate a minimum of three different positions for nucleosome cores relative to the alpha-satellite sequence and suggest a random distribution in at least some regions. in addition, while we confirm published reports that staphylococcal nuclease cuts the alpha-satellite ...19846089117
analysis of two potential shuttle vectors containing herpes simplex virus defective dna.two potential shuttle vectors which contained the identical herpes simplex virus type 1 (hsv-1) defective particle dna (ddna), but prokaryotic dna of different origins, were examined for their stability when propagated in eukaryotic cells, and for their efficiency as shuttle vectors. each chimeric molecule contained a 9.5 kilobase-pair (kb) ecori fragment (hsv12-7) representing a single unit of a class i hsv-1 ddna. this ddna was cloned into the bacteriophage lambda (lambda) vector lambda gtwes ...19846090565
continuous reorganization leads to extensive polymorphism in a monkey centromeric satellite.previously we reported the existence of a highly polymorphic satellite, deca-satellite, in the african green monkey genome; deca-satellite probe anneals to complex sets of repeated restriction endonuclease fragments that differ from individual to individual in the monkey population. here we present experiments aimed at clarifying the structure and organization of deca-satellite sequences and investigating the mechanisms that generate the polymorphisms. deca-satellite represents less than 1% of t ...19846094831
conservation of the human col1a1-tk-gaa synteny and homoeologous assignment in the african green monkey and the baboon (cercopithecoidae).the pro alpha 1 (i) collagen structural gene (col1a1), the acid alpha-glucosidase (gaa), and the thymidine kinase (tk) genes, known to be closely linked in man (hsa) and mapped to hsa17, were found syntenic in two cercopithecoidae species, the baboon (papio papio, ppa) and the african green monkey (cercopithecus aethiops, cae) and assigned to homoeologous chromosomes, ppa16 and cae19, respectively. the assignment of col1a1 was obtained using two human cdna probes. hind iii restriction sites foun ...19846096258
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