Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| trypanosoma cruzi glycoprotein 72: immunological analysis and cellular localization. | two monoclonal antibodies were used to biochemically characterize glycoprotein 72 (gp72) from trypanosoma cruzi and to localize the protein in live and fixed parasites by indirect immunofluorescence and in thin section of parasites by immunogold electron microscopy. gp72 was shown in immunoblots to be specific for the epimastigote stage; the protein could not be detected in trypomastigotes. each antibody reacted with a different epitope on the glycoprotein and deglycosylation of gp72 ablated rea ... | 1992 | 1614419 |
| identification and characterization of two repetitive non-variable antigens from african trypanosomes which are recognized early during infection. | the present paper describes two repetitive proteins representing common antigens of african trypanosomes which are non-variant and which are recognized early in infection by the host immune system. these antigens were identified by their ability to immunoreact with bovine serum taken during the early phase of a cyclic trypanosomal infection. screening of a cdna library from t. b. gambiense with such early infection serum identified a protein which contains a repetitive motif consisting of 68 ami ... | 1992 | 1614728 |
| trypanosoma cruzi glutathione-binding proteins: immunogenicity during human and experimental chagas' disease. | following purification by affinity chromatography, three glutathione-binding proteins (tcgbp) of 45, 30, and 25 kda were co-purified from trypanosoma cruzi epimastigotes. using 1-chloro-2,4 dinitrobenzene as substrate, a glutathione s-transferase activity of 70 nmol/min/mg of proteins was detected in the gsh binding fraction. an increased expression of tcgbp and total gst activity was observed upon incubation of parasites with phenobarbital, which is an inducer of gst synthesis. immunofluorescen ... | 1992 | 1614743 |
| nucleotide sequence of a cdna encoding another trypanosoma cruzi acidic ribosomal p2 type protein (tcp2b). | 1992 | 1614880 | |
| nucleotide sequence of a cdna encoding a trypanosoma cruzi acidic ribosomal po protein: a novel c-terminal domain in t. cruzi ribosomal p proteins. | 1992 | 1614881 | |
| inhibitors of the major cysteinyl proteinase (gp57/51) impair host cell invasion and arrest the intracellular development of trypanosoma cruzi in vitro. | peptidyl diazomethane (pdam) derivatives, a class of irreversible inhibitors for cysteine proteinase, were screened for the ability to impair trypanosoma cruzi invasion and intracellular development in primary cultures of heart muscle cells (hmc). t. cruzi gp57/51, a purified cysteinyl proteinase, and the substrate z-phe-arg-nhmec were used to determine inhibition rate constants (k'+2) by continuous kinetic assays. the k'+2 values ranged from 25,400 to 2,800. the best inhibitors of gp57/51 had b ... | 1992 | 1620157 |
| calcium homeostasis in trypanosoma cruzi amastigotes: presence of inositol phosphates and lack of an inositol 1,4,5-trisphosphate-sensitive calcium pool. | the permeabilization of trypanosoma cruzi amastigotes with digitonin allowed the study of ca2+ fluxes between intracellular organelles in situ. in addition, fura-2 was used to determine the cytosolic ca2+ concentration in the intact cells. when amastigotes were permeabilized in a reaction medium containing mgatp, succinate and 3.5 microm ca2+, they lowered the medium ca2+ concentration to the submicromolar level, a range which correlates favorably with that detected in the intact cells with fura ... | 1992 | 1620163 |
| the pharmacokinetics of nifurtimox in chronic renal failure. | the pharmacokinetics of nifurtimox, a drug used in the treatment of trypanosoma cruzi infections, has been studied in seven patients with chronic renal failure undergoing haemodialysis, and in seven healthy subjects. each subject took nifurtimox 15 mg.kg-1 orally and blood samples were obtained for 10 h after administration. nifurtimox in serum was analyzed by hplc. the patients with chronic renal failure had a higher cmax than the control subjects due to a change in systemic availability. an al ... | 1992 | 1623911 |
| trypanosoma cruzi 5s rrna genes: molecular cloning, structure and chromosomal organization. | to further study the ribosomal rna genetic system in trypanosoma cruzi, the 5s rrna gene family was characterized. we found that this gene family is reiterated about 1600 times per diploid nuclei and is mostly organized as a tandem repeat of 481 base pairs. these gene clusters were assigned to two chromosomes of about 1500 and 1400 kilobase pairs. we found that the 5s rrna-coding region is comprised of 120 nucleotides, and contains the well-known internal control regions of eukaryotic rna polyme ... | 1992 | 1624112 |
| [seroepidemiology of chagas disease in mexico]. | the lack of information about chagas disease in mexico, as well as the controversy concerning its importance, was the basis for the seroprevalence study of trypanosoma cruzi in the national seroepidemiology survey (nss). this information was representative of the national situation with regard to disease prevalences and other factors related to the nation's health. unfortunately the nss was not a very good information source for the study of trypanosomiasis americana, because its coverage in the ... | 1992 | 1631732 |
| transcoronary chemical ablation of ventricular tachycardia in chronic chagasic myocarditis. | a case of incessant ventricular tachycardia in a patient with chagas' disease that was successfully terminated by chemical ablation is reported. | 1992 | 1634689 |
| changes in humoral responses to trypanosoma cruzi during the course of infection in mice held at elevated temperature. | a parasite-specific, enzyme-linked immunosorbent assay and immunoblot analysis were used to examine the development of humoral immunity in trypanosoma cruzi-infected c3h mice that survive acute infection when held at elevated environmental temperature. both parasite-specific antibody levels and numbers of antigens identified increased during infection in mice held at 36 c, with the greatest reactivity measured in sera from mice that had resolved parasitemias. heat shock of culture forms of t. cr ... | 1992 | 1635028 |
| binding of complement to trypomastigotes of a brazil strain of trypanosoma cruzi: evidence for heterogeneity within the strain. | binding of the complement components c3 and c5 to epimastigote and trypomastigote stages of the brazil strain of trypanosoma cruzi was examined using radioligand binding and flow cytometric assays. fibroblast-derived trypomastigotes bound approximately 40% fewer molecules of [125i]c3 per parasite than did epimastigotes. the predominant molecular species of c3 deposited on fibroblast-derived trypomastigotes was the inactive form ic3b. addition of parasite-specific antisera failed to enhance the n ... | 1992 | 1635029 |
| vectorial transmission of trypanosoma cruzi: an experimental field study with susceptible and immunized hosts. | the dynamics of vectorial transmission of trypanosoma cruzi and the level of host (guinea pigs) protection after immunization with attenuated parasites (tcc strain) was studied under natural climatic conditions in an endemic region of northern argentina. the experimental design included two guinea pig corrals isolated by mosquito netting. one (controls) had 17 healthy and susceptible adult guinea pigs. the other had 19 guinea pigs immunized with attenuated t. cruzi tcc strain. each corral was co ... | 1992 | 1636879 |
| serologic screening for trypanosoma cruzi among blood donors in central brazil. | the study reported here compares results obtained by blood banks screening sera for chagasic (trypanosoma cruzi) infection with results obtained by the chagas' disease reference laboratory of the federal university of goiás in goiânia, brazil. it also evaluates results obtained using the elisa technique to screen the study sera. the survey used data from six of eight blood banks serving the city of goiânia, an urban region of central brazil where chagas' disease is highly endemic. the survey pop ... | 1992 | 1638280 |
| cardiac autonomic dysfunction and neuroganglionitis in a rat model of chronic chagas' disease. | the aim was to evaluate whether the cardiac parasympathetic function in a rat model of chronic chagas' disease is impaired as in the human disease, and to correlate the functional state to histopathology of the intrinsic autonomic innervation of heart. | 1992 | 1638561 |
| trypanosoma cruzi: involvement of igg isotypes in the parasitemia control of mice immunized with parasite exoantigens of isoelectric point 4.5. | in a previous work we demonstrated that trypanosoma cruzi exoantigens of pi 4.5 (ea 4.5), whose most important epitopes are glucidic, are able to induce a partially protective immune response in mice. to ascertain the involvement of antibody isotypes in this protection, we immunized mice with ea 4.5 plus bordetella pertussis as adjuvant. the analysis of immune response by skin test revealed the occurrence of specific immediate type hypersensitivity on day 15 after the last immunization. by elisa ... | 1992 | 1639159 |
| purification of a trypanosoma cruzi trypomastigote 60-kilodalton surface glycoprotein that primes and activates murine lymphocytes. | we have purified a glycoprotein with a relative molecular mass of 60 kda and present on the surface of trypanosoma cruzi trypomastigotes and studied its ability to prime and stimulate the proliferation of murine spleen cells. t. cruzi trypomastigote membrane proteins were separated by preparative isoelectrofocusing. a trypomastigote 60-kda surface protein with an isoelectric point of 4.2 was enriched by chromatofocusing and was readily purified in native form to homogeneity by gel filtration on ... | 1992 | 1639469 |
| ventricular tachycardia in chagas' disease. | this study examined the usefulness of the electrophysiologic approach for selecting antiarrhythmic drug therapy to improve survival in patients with ventricular tachycardia (vt) and chagas' disease. a total of 71 consecutive chagasic patients undergoing treatment and evaluation of vt were analyzed. programmed electrical stimulation (pes) was performed in 45 patients, sustained vt was induced in 18 of these 45 (40%); nonsustained vt was induced in 17 (38%), and in 10 patients (22%) vt was not ind ... | 1992 | 1642183 |
| [serological screening for trypanosoma cruzi among blood donors in central brazil]. | the present study compares the results of serological screening for trypanosoma cruzi infection done at blood banks with results obtained in chagas' disease studies undertaken by the reference laboratory of the federal university of goiás (ufg) and evaluates the use of the enzyme-linked immunosorbent assay (elisa) for this purpose. the study was conducted with data from six of the eight blood banks in the city of goiânia in central brazil, an urban area in which this infection is highly endemic. ... | 1992 | 1642781 |
| chagas cardiomyopathy and captopril. | chagas disease is a leading cause of heart failure in latin america. sudden death occurs in approximately 40% of patients with heart failure due to chagas disease. we report a single blind, cross-over trial of prolonged treatment with captopril and placebo in 18 chagas disease patients with class iv nyha heart failure. ventricular dimensions, neurohormones, electrolytes and ventricular arrhythmias were analysed in 11 men and seven women receiving stable doses of digoxin and frusemide who were ra ... | 1992 | 1644089 |
| extremely divergent histone h4 sequence from trypanosoma cruzi: evolutionary implications. | trypanosoma cruzi presents six histones electrophoretically resolved in three gel systems. indirect evidence shows that one of these histones, named e, corresponds to h4 in other species. we present evidence that histone e is h4 by sequencing its amino terminal end. the amino terminal of t. cruzi histone h4, unlike that of other h4s examined thus far is not blocked. moreover, this protein presents two variants. this partial amino acid sequence of t. cruzi histone h4 differs greatly from homologo ... | 1992 | 1644863 |
| myocardial beta-adrenergic adenylate cyclase complex in a canine model of chagasic cardiomyopathy. | infection of beagles with an opossum-derived strain of trypanosoma cruzi (tc-o) results in features of early and chronic chagasic cardiomyopathy, that is, increases in pr interval, atrioventricular block, and frequent ventricular premature contractions, ventricular tachycardia, and decreased left ventricular ejection fraction. these signs are not observed in animals infected with a canine strain of t. cruzi (tc-d). to understand the biochemical basis for these early cardiac effects, we examined ... | 1991 | 1647278 |
| trypanosoma cruzi attachment to lymphocyte muscarinic cholinergic and beta adrenergic receptors modulates intracellular signal transduction. | plasma membrane vesicles of trypanosoma cruzi (pmvs) formed saturation binding isotherms with naive murine t lymphocytes. parasite membrane attachment to the muscarinic cholinergic receptors of lyt 2.2+t cells (suppressor cells) resulted in the synthesis of cgmp, attenuation of camp levels and in the secretion of prostaglandin e2, an immunoregulator effector substance. these t suppressor cell signals were blunted by atropine and by monospecific antibody against t. cruzi surface epitopes. the int ... | 1991 | 1649970 |
| trypanosoma cruzi but not trypanosoma brucei fails to induce a chemiluminescent signal in a macrophage hybridoma cell line. | macrophage-trypanosoma cruzi interactions were studied by using a newly generated macrophage hybridoma cell line (2c11-12) that was selected for its capacity to produce high levels of reactive oxygen intermediates. this cell line was found to be a suitable host cell for t. cruzi, and intracellular parasitic development could be inhibited by activation with gamma interferon. when exposed to opsonized trypanosoma brucei, micrococcus lysodeikticus, or legionella pneumophila, the activated macrophag ... | 1991 | 1652563 |
| a novel t. cruzi heparin-binding protein promotes fibroblast adhesion and penetration of engineered bacteria and trypanosomes into mammalian cells. | t. cruzi invades mammalian cells in various organs after migrating through the ecm. these activities appear to be mediated by a unique 60 kd protein exposed on the t. cruzi surface, which promotes selective adhesion of trypomastigotes to three ecm components: heparin, heparan sulfate, and collagen. the affinity-purified protein binds to host fibroblasts in a saturable and glycosaminoglycan- and collagen-inhibitable manner. when adsorbed to plastic, it promotes adhesion and spreading of fibroblas ... | 1991 | 1655283 |
| endocytosis of gold-labeled proteins and ldl by trypanosoma cruzi. | endocytosis was studied at the ultrastructural level in different developmental forms of trypanosoma cruzi after incubation of the parasites in the presence of gold-labeled proteins (albumin-au, peroxidase-au and transferrin-au) and low-density lipoprotein (ldl-au). epimastigote (culture) forms actively ingested ldl and proteins. initially, gold particles were seen adhering only to the cytostome and inside the flagellar pocket. in parasites incubated at 4 degrees c with transferrin-au or peroxid ... | 1991 | 1656428 |
| chagasic igg binding with cardiac muscarinic cholinergic receptors modifies cholinergic-mediated cellular transmembrane signals. | the interaction of the igg from trypanosoma cruzi-infected mice (chagasic igg) with cardiac cholinergic receptors by means of specific radioligand binding and by production of cholinergic-mediated cellular transmembrane signals was characterized. chagasic igg inhibited, in a noncompetitive manner, the binding of [3h]quinuclidinyl benzilate to the cardiac membrane. moreover, chagasic igg could modify all of the muscarinic cholinergic effects mediated by a g regulatory protein, i.e., decrement of ... | 1991 | 1657467 |
| octadecyl silica: a solid phase for protein purification by immunoadsorption. | immunoaffinity chromatography involves binding of an antigen or antibody to a solid matrix, usually agarose, frequently using the cyanogen bromide method. these methods are laborious, rather expensive, and their use has been mostly restricted to immunopurifications on the microscale. we propose here the use of octadecyl silica (sicl8) beads, a matrix for hplc, as an alternative solid phase for protein immunopurification and immunoadsorption. antibodies or antigens are strongly bound to sicl8 by ... | 1991 | 1659249 |
| polymerase chain reaction amplification of trypanosoma cruzi kinetoplast minicircle dna isolated from whole blood lysates: diagnosis of chronic chagas' disease. | a 6 m guanidine-hcl/0.2 m edta solution was used to lyse and store whole blood specimens. dna stored in guanidine-edta-blood (geb) lysate was found to be undegraded after incubation at 37 degrees c for 1 month, suggesting that this represents an appropriate reagent for transport of blood samples from the field to a laboratory for analysis. trypanosoma cruzi kinetoplast dna in geb lysate can be cleaved using the chemical nuclease, 1,10-phenanthroline-copper ion (op-cu2+). this procedure liberates ... | 1991 | 1662334 |
| evidence of absence of trypanosoma cruzi kinetoplast dna methylation by restriction endonuclease analysis. | eight kinetoplast dna samples from very different t. cruzi zymodemes were digested with the isoschizomer group of enzymes (mspi-hpaii) and (mboi-sau 3ai), able to detect dna methylation on cytidine and adenine for the ccgg and gatc sequences, respectively. restriction digestion analysis of each kdna with both isoschizomer groups of enzymes did not display a different profile suggesting that maxicircles and minicircles on this trypanosomatid are not methylated. | 1991 | 1663492 |
| [effect of lipophilic ortho-naphthoquinones on the growth of and production of peroxides by leptomonas seymouri and crithidia fasciculata]. | the lipophilic o-naphthoquinones cg 8-935, cg 9-442, cg 10-248 and mansonones a, c, e and f inhibit growth of the trypanosomatids leptomonas seymouri (ls) and crithidia fasciculata (cf). the most active mansonones were e and f (i50, 0.1-0.4 microm with ls; 0.3-1.2 microm with cf) with cytotoxic activities equal to or higher than quinones cg, as reported previously. incubation of ls or cf with the cg-quinones and mansonones e and f caused the release of h2o2 and o2-. from the whole cells to the s ... | 1991 | 1667695 |
| [trypanosoma cruzi: induction of changes in the peripheral nervous system in different strains of mice]. | this study has been designed to find an easy method to evaluate the motor unit alterations induced during experimental t. cruzi infections. different mouse strains infected with three strains of t. cruzi were used to perform conventional needle electromyography, in one of the lower limb hamstring muscles; amplitude, duration and number of phases of single motor unit potentials were measured. the following parasite strain to mouse strain relationship was investigated, in mice inoculated intraperi ... | 1991 | 1667698 |
| [insertion of trypanosoma cruzi dna in the genome of mammal host cell through infection]. | trypanosoma cruzi chromatin was observed in macrophage chromosome spreads obtained at different periods of infections in balb/c mice. immunofluorescent studies showed that genetic materials associated with the chromosomes were t. cruzi products. in situ hybridization showed the protozoon dna insertion in the host cell genome. in addition, selective 3h-dna insertion in chromosomes 3, 6 and 11 was observed, which suggested that transxenogene rearrangement may take place in t. cruzi infections of m ... | 1991 | 1667709 |
| detection and classification of trypanosoma cruzi by dna hybridization with nonradioactive probes. | total or kinetoplast dna (kdna) from 72 isolates and clones of trypanosoma cruzi as well as from nine related trypanosomatids were analyzed by dot hybridization using nonradioactive kdna or cloned minicircle fragments as probes. biotinylated-kdna probes generated by nick-translation proved reliable for distinguishing zymodeme 1 and zymodeme 2bol of t. cruzi parasites. in contrast, digoxigenin-labeled kdna obtained by random-priming did not distinguish among t. cruzi isolates but did distinguish ... | 1991 | 1667933 |
| peripheral motor nerve conduction studies in patients with chronic chagas' disease. | a comprehensive investigation of nerve motor conduction velocity was carried out in the ulnar nerve of 70 patients in the chronic stage of chagas disease. it was found that 2.6% of them, had slowness of the large motor axons at distal segments and 14% at proximal segments. twenty seven percent of them had also slowness of conduction at the smallest motor fibers. these observations signal that some patients in the chronic stage of the infection may develop a motor neuropathy. | 1991 | 1668781 |
| stability of isoenzyme and kinetoplast dna (k-dna) patterns in successively cloned trypanosoma cruzi populations. | four trypanosoma cruzi strains from zymodemes a, b, c and d were successively cloned on bhi-lit-agar-blood (blab). twenty clones from the first generation (f1), 10 from the second (f2) and 4 from the third (f3) from the strains a138, b147 and c231 were isolated. the d150 strain provided 29 f1 and 23 f2 clones. the strains and clones had their isoenzyme and k-dna patterns determined. the clones from a138, b147 and c231 strains presented isoenzyme and k-dna patterns identical between themselves an ... | 1991 | 1668791 |
| biological false positive test for syphilis in lepromatous leprosy patients with concomitant hepatitis b virus infection. | within the wide range of immunological abnormalities occurring in lepromatous leprosy (ll), antiphospholipid antibodies unrelated to treponemal infection, the so-called biological false positive test for syphilis (bfp), have long been recognized. considering that bfp may also be encountered in different clinical situations, the fact that whether bfp in ll occurs solely or is associated with other concomitant infections or serological abnormalities was investigated. for this purpose, two groups o ... | 1991 | 1669564 |
| neuroendocrine design of the gut. | the enteric nervous system (ens) can be thought of as the third component of the autonomic nervous system. it is a vast network of neurons widely dispersed throughout the gut. the ens is a dominant regulator of gut function through the action of peptide and non-peptide neurotransmitters. the most intensively studied roles of the ens have been the regulation of secretory processes, such as gastric acid secretion, and motility. it is clear, however, that the ens plays a broader role in the regulat ... | 1991 | 1671322 |
| studies on the thymus in chagas' disease. ii. thymocyte subset fluctuations in trypanosoma cruzi-infected mice: relationship to stress. | changes in thymic t-cell subsets in mice acutely infected with trypanosoma cruzi have been studied in both c3h/hej and c57bl/6 mice. the significant decrease in thymocyte number, observed in both mouse strains on day 14 post-infection correlated with a drastic decrease in cd4+cd8+ cell number, whereas the number of cd4-cd8-, cd4+cd8- and cd4-cd8+ cells remained essentially unchanged. the important increase in cd3hi cell frequency confirmed that resistant thymocytes during chagas' disease develop ... | 1991 | 1672774 |
| morphometric investigations of the colon mucosa in chronic trypanosoma cruzi infected rats. | the objective of this investigation was to study the morphometry of the epithelial mucosa in the chronic phase of t. cruzi infection. nine young female wistar rats were inoculated with t. cruzi. ten months after inoculation the animals were sacrificed and the proximal colon was collected for morphometric measurements of the thickness of the muscle layers, the number of neurons in the myenteric plexus, the crypt cell population (ccp), crypt cell production per crypt (ccpc) and turnover time (tt) ... | 1991 | 1675508 |
| are eukaryotic microorganisms clonal or sexual? a population genetics vantage. | we argue that the mode of reproduction of microorganisms in nature can only be decided by population genetic information. the evidence available indicates that many parasitic protozoa and unicellular fungi have clonal rather than sexual population structures, which has major consequences for medical research and practice. plasmodium falciparum, the agent of malaria, is a special case: the scarce evidence available is contradictory, some suggesting that uniparental lineages may exist in nature. t ... | 1991 | 1675793 |
| the role of t-cell subpopulations in experimental chagas' disease. | 1991 | 1678199 | |
| murine trypanosoma cruzi infection: a role for th2 cells in the immunopathology of chronic infection. | 1991 | 1678201 | |
| acute trypanosoma cruzi infection differentially affects cd3 and thy-1 mediated t cell activation. | monoclonal antibodies directed against the thy-1 molecule or the cd3 complex were used to analyze the activation of t cells from mice acutely infected with trypanosoma cruzi. when stimulated with g7, a mitogenic anti-thy-1 monoclonal antibody, spleen cells from infected mice showed a markedly reduced or absent response that could not be restored by varying the culture time or the antibody concentration. however, cells from acutely infected animals proliferated to 145-2c11, an anti-cd3 monoclonal ... | 1991 | 1679416 |
| trypanosoma cruzi: flow cytometric analysis of lymphocyte subsets in susceptible and protected c3h/he mice. | inbred strains of mice vary widely in their ability to survive infection with trypanosoma cruzi. c3h/he mice are highly susceptible to infection with the brazil strain t. cruzi, but can be protected by immunization with avirulent corpus christi strain parasites. we have examined, during the course of infection, the changes in lymphocyte populations in c3h/he mice that were infected but protected by immunization, infected but not immunized, immunized but not infected, and normal age-matched contr ... | 1991 | 1679718 |
| comparative studies of nifurtimox uptake and metabolism by drug-resistant and susceptible strains of trypanosoma cruzi. | 1. nifurtimox uptake and metabolism by epimastigote forms of three strains of trypanosoma cruzi (basileu, y, yuyu) with different drug responsiveness in mice experimental infections were compared. 2. statistical analysis of the results demonstrated no correlation between the ability of the strains to catalyze nifurtimox redox-cycling (basileu = y = yuyu) nor nifurtimox multiple electron reduction (basileu = y greater than y) and drug susceptibility (basileu greater than y greater than yuyu). 3. ... | 1991 | 1685402 |
| characterization of trypanosoma cruzi from argentina by electrophoretic zymograms. | polyacrylamide gel electrophoretic patterns for six enzymes in 73 isolates and 38 clones of trypanosoma cruzi from different areas of argentina were classified into 12 zymodemes. the isolates were obtained from human patients with acute, chronic or congenital chagas' disease, vector insects, domestic and sylvatic animals. two out of 8 isolates cloned were shown to be heterogeneous. zymodemes 1 and 12 exhibit widespread geographic distribution; isolates belonging to both zymodemes account for 55% ... | 1991 | 1685868 |
| a glass wool-based method for purifying trypanosoma cruzi trypomastigotes and identification of an epimastigote-specific glass-adherent surface peptide. | glass wool, hydrophilic cotton wool, non-electrically charged bio-gel p2 and common tissue paper columns were used to purify trypomastigotes from a mixed trypanosoma cruzi population grown in axenic culture medium. with all these columns, highly purified (up to 98%) trypomastigote preparations were obtained. trypomastigote yields from cotton wool, bio-gel p2 and common tissue paper columns were not as high as from glass wool columns, from which yields varied from 69 to 80%. purification on glass ... | 1991 | 1686142 |
| rapid isolation of metacyclic trypomastigotes of trypanosoma cruzi from feces and urine of the vector. | metacyclic trypomastigotes of trypanosoma cruzi from infected triatoma infestans were isolated and purified by an improved procedure. by using feces and urine of bugs the duration and the number of steps for purification were greatly reduced, thereby minimizing any possible effects on the flagellates. after deae-sephacel column chromatography, a buffer supplemented with bovine serum albumin protected against the slight surface alterations of the trypomastigotes otherwise observed. about 10(7) me ... | 1991 | 1686144 |
| phloretin and citrate promote the differentiation rate from epimastigote to metacyclic forms of trypanosoma cruzi. | we have investigated the effects of the metabolic inhibitors, phloretin, 2-deoxy-d-glucose, maleate and trans-aconitate, as well as two intermediates of the tricarboxylic-acid cycle, acetate and citrate, on the growth and metacyclogenesis of trypanosoma cruzi. 0.1 mm phloretin increased the percentage of metacyclic forms about 2.7-fold without affecting growth rate, whereas the other inhibitors had no apparent effect on either growth or differentiation rates. the addition of 5 mm citrate stimula ... | 1991 | 1686147 |
| preventing transfusion-transmitted infections: issues related to migrating populations and increasing world travel. | blood donations by emigrants or other travelers from distant lands require special attention for the prevention of transfusion-transmitted infections. the human immunodeficiency virus, type 1 (hiv-1) is only one of several bloodborne viruses that may be introduced into non-epidemic areas by transfusion and spread further by sexual intercourse, needles shared by drug users or mother-child transmission. although transfusion-transmitted parasites, such as trypanosoma cruzi, do not spread further in ... | 1991 | 1688227 |
| expression and polymorphism of a trypanosoma cruzi gene encoding a cytoplasmic repetitive antigen. | the study of the expression of a trypanosoma cruzi gene encoding a cytoplasmic repetitive antigen (cra) during the metacyclogenesis process shows that this gene is not expressed in metacyclic trypomastigote forms of the parasite. however, a slight increase in cra expression was observed following the nutritional stress of epimastigotes which precedes t. cruzi metacyclogenesis in vitro. the comparison of the expression of cra in different t. cruzi strains shows that this gene is highly polymorphi ... | 1990 | 1690143 |
| expression in escherichia coli of a dominant immunogen of trypanosoma cruzi recognized by human chagasic sera. | a genomic clone expressing a trypanosoma cruzi antigen in escherichia coli was identified using human chagasic sera. chagasic antibodies affinity purified on extracts of this clone recognized a high-molecular-weight protein expressed in all developmental stages of the parasite life cycle, as well as in various t. cruzi strains. the antigen is associated with the cytoskeleton of the parasite and localizes along the attachment region between the flagellum and the cell body. antibodies to the recom ... | 1990 | 1691209 |
| cloning and characterization of a gene coding for a protein (kap) associated with the kinetoplast of epimastigotes and amastigotes of trypanosoma cruzi. | we have cloned and characterized a gene of trypanosoma cruzi which encodes a protein, kap (kinetoplasts-associated protein), expressed in the kinetoplasts of epimastigotes and amastigotes, the replicative stages of the parasite, but not in kinetoplasts of trypomastigotes. the single-copy gene is transcribed into a 3900-nt polyadenylated mrna. its trans-splicing acceptor site is preceded by a run of 15 adenosine residues. an open reading frame of 1052 codons is followed by a 3' untranslated regio ... | 1990 | 1694571 |
| trypanosoma cruzi: differential expression and distribution of an 85-kda polypeptide epitope by in vitro developmental stages. | the expression by trypanosoma cruzi developmental stages of an 85-kda polypeptide epitope defined by the 155d3 monoclonal antibody (mab) has been investigated. immunoprecipitation revealed the presence of an 85-kda antigen in the np-40 soluble extract of parasites freshly released from infected fibroblasts; this antigen was not found in epimastigote and leishmania infantum promastigote. indirect immunofluorescence revealed that the mab 155d3 failed to react with trypomastigotes, whereas extracel ... | 1990 | 1695578 |
| the cloned c-terminal region of a trypanosoma cruzi p ribosomal protein harbors two antigenic determinants. | a dna strategy was designed to characterize the antigenic site(s) within a lambda gt11 cloned 35-amino-acid antigenic peptide, identified with antibodies from patients with chronic chagas' heart disease (cchhd) and systemic lupus erythematosus (sle) as the c-terminal portion of a trypanosoma cruzi p ribosomal protein. the 198-bp cdna insert was digested with alui, resulting in two dna segments that were recloned in lambda gt11. to identify specific antigenic determinants, the recombinant phage a ... | 1990 | 1695612 |
| the major 85-kd surface antigen of the mammalian form of trypanosoma cruzi is encoded by a large heterogeneous family of simultaneously expressed genes. | trypanosoma cruzi is an obligate intracellular protozoan parasite. the parasite mammalian stage surface antigens exhibit extensive antigenic diversity. we have characterized a family of t. cruzi genes that code for a polymorphic set of 85-kd surface antigens, the sa85-1 antigens. the family contains greater than 100 genes and pseudogenes, of which a minimum of nine are transcribed. the gene family is expressed in the mammalian stage only. a subset of the gene family is present in two telomere-li ... | 1990 | 1695668 |
| major trypanosoma cruzi antigenic determinant in chagas' heart disease shares homology with the systemic lupus erythematosus ribosomal p protein epitope. | a trypanosoma cruzi lambda gt11 cdna clone, jl5, expressed a recombinant protein which was found to react predominantly with chronic chagas' heart disease sera. the cloned 35-residue-long peptide was identified as the carboxyl-terminal portion of a t. cruzi ribosomal p protein. the jl5 13 carboxyl-terminal residues shared a high degree of homology with the systemic lupus erythematosus (sle) ribosomal p protein epitope. synthetic peptides comprising the 13 (r-13), 10 (r-10), and 7 (r-7) carboxyl- ... | 1990 | 1696282 |
| a galactosyl(alpha 1-3)mannose epitope on phospholipids of leishmania mexicana and l. braziliensis is recognized by trypanosomatid-infected human sera. | an immunoglobulin m antibody reactive with galactosyl(alpha 1-3)mannose [gal(alpha 1-3)man] residues present on phospholipids extracted from leishmania mexicana and l. braziliensis was found to be present in high titer in the serum of every normal individual studied. periodate oxidation, acid hydrolysis, or acetylation suppressed immunoreactivity, suggesting that an oligosaccharide chain was responsible for antibody binding. interaction occurs only with alpha-gal terminal residues, since treatme ... | 1990 | 1696285 |
| analyses of surface membrane carbohydrates in parasitic flagellates of the order kinetoplastida using lectins. | crithidia fasciculata, leishmania donovani, leishmania major, leishmania mexicana amazonensis, leishmania tropica, leishmania tarentolae, trypanosoma sp. from formosan bats (tb), trypanosoma lewisi, trypanosoma musculi, and different strains of trypanosoma cruzi (tc) were cultivated at 27 degrees c in a liquid culture medium. flagellates harvested from log phase culture were analyzed for their lectin agglutinating characteristics with concanavalin a (con a), peanut agglutinin, ricinus communis a ... | 1990 | 1696387 |
| characterization of a cdna encoding a cysteine-rich cell surface protein located in the flagellar pocket of the protozoan trypanosoma brucei. | we have characterized a cdna encoding a cysteine-rich, acidic integral membrane protein (cram) of the parasitic protozoa trypanosoma brucei and trypanosoma equiperdum. unlike other membrane proteins of t. brucei, which are distributed throughout the cell surface, cram is concentrated in the flagellar pocket, an invagination of the cell surface of the trypanosome where endocytosis has been documented. accordingly, cram also locates to vesicles located underneath the pocket, providing evidence of ... | 1990 | 1697030 |
| permeability alteration of the sarcolemmal membrane, particularly at the site of macrophage contact, in experimental chronic trypanosoma cruzi myocarditis in mice. | sarcolemmal membrane permeability characteristics have been investigated, particularly at the site of macrophage contact, in experimental chronic trypanosoma cruzi myocarditis in balb/c mice, employing ruthenium red (rr) as an electron tracer. the ultrastructural features of the myocardium from infected animals were similar to those previously described. briefly, focal myocarditis was detected, with areas of myocytolytic necrosis, atrophic myofibres, an inflammatory response composed of mononucl ... | 1990 | 1698094 |
| antigen-specific t-helper cells abrogate suppression in trypanosoma cruzi-infected mice. | the ability of antigen-specific t-helper (th) cells to enhance direct plaque-forming cell responses in spleen cells from trypanosoma cruzi-infected c57bl/6 mice was investigated at various times during the course of infection from day 7 to day 230. the injection of antigen-specific th cells in vivo or the addition of antigen-specific th cells in vitro was effective in enhancing direct plaque-forming cell responses, except at the time of the most intense suppression during the acute phase of infe ... | 1990 | 1698177 |
| trypanosoma cruzi: cloning and expression of an antigen recognized by acute and chronic human chagasic sera. | here we describe the characterization of a trypanosoma cruzi dna sequence (clone a13) that codes for a polypeptide recognized by igm and igg antibodies from sera of acute and congenital chagasic patients. antibodies to a13 antigen are also detected in the sera of chronic patients with different clinical forms of chagas' disease, but not in sera of patients with leishmaniasis or other parasitic diseases. the antigenic determinants encoded by clone a13 are found in amastigotes and trypomastigotes ... | 1990 | 1698656 |
| presence of anti-trypanosoma cruzi antibodies in the sera of mice with experimental autoimmune myocarditis. | the existence of antigens shared in common by t. cruzi and heart muscle cells is suggested by the presence of antibodies binding to the parasite surface in the serum of mice with autoimmune myocarditis induced by immunization with syngenic heart antigens. | 1990 | 1698659 |
| analysis of antibody cross-reactivity in experimental american trypanosomiasis. | susceptible c3h/he mice were immunized with the avirulent corpus christi strain of trypanosoma cruzi and subsequently infected with virulent brazil stain organisms. seventy days after infection sera were isolated and analyzed on western blots of electrophoretically separated t. cruzi antigens prepared from culture-form parasites (primarily epimastigotes). more than 25 bands were identified. the antibodies were fractionated by elution from various regions of western blots corresponding to average ... | 1990 | 1698959 |
| trypanosoma cruzi: killing and enhanced uptake by resident peritoneal macrophages treated with alpha-2-macroglobulin. | we report that alpha-2-macroglobulin (a2m), the physiologically important plasma protease inhibitor and suspected immunomodulator, alters the functional ability of murine resident peritoneal macrophages (rm) to ingest and kill the infective trypomastigote stage of trypanosoma cruzi, the aetiological agent of chagas' disease. treatment of rm with 500 micrograms/ml a2m for 30 min enhanced the uptake of trypomastigotes, epimastigotes, and amastigotes by 125%, 46%, and 300%, respectively. the same t ... | 1990 | 1699221 |
| myocardial damage in trypanosoma cruzi myocarditis: a role for macrophages. | sarcolemmal membrane permeability characteristics, particularly at the site of macrophage contact, were investigated in experimental chronic trypanosoma cruzi myocarditis in balb/c mice, employing ruthenium red as an electron tracer. the ultrastructural features of the myocardium from infected animals were similar to those previously described. briefly, focal myocarditis was detected, with areas of myocytolytic necrosis, atrophic myofibres, an inflammatory response composed of mononuclear cells, ... | 1990 | 1699645 |
| extracellular matrix derived from trypanosoma cruzi infected endothelial cells directs phenotypic expression. | infection of confluent human umbilical vein endothelial cells by the parasite trypanosoma cruzi results in the appearance of an altered heparan sulfate proteoglycan (hspg) isolated from the extracellular matrix of infected endothelial cells (ecmi). hspg from ecmi differed from hspg obtained from the extracellular matrix of uninfected endothelial cells (ecmu) by virtue of an 8-10-fold increase in sulfation and a different elution pattern using deae sepharose chromatography. analysis of the hspg t ... | 1990 | 1700983 |
| trypanosoma cruzi: plasma levels of alpha-2-macroglobulin during experimental murine infections with reticulotropic and myotropic strains. | 1990 | 1701252 | |
| isolation and immunological analysis of trypanosoma cruzi glycolipids. | a water-soluble glycolipidic fraction from trypanosoma cruzi was isolated using a mixture of hexane and isopropanol. analysis by sds-polyacrylamide gel electrophoresis, after staining by silver and sudan black b, showed that the fraction contained one band with a relatively high mobility. its reactivity and specificity with human chagasic sera and t. cruzi infected mouse sera or with sera from patients with several other pathologies was determined by an immunoradiometric assay. the glycolipid-ba ... | 1991 | 1704684 |
| structural and functional identification of gp57/51 antigen of trypanosoma cruzi as a cysteine proteinase. | purified gp57/51, a trypanosoma cruzi glycoprotein earlier identified as a major antigen in infected humans, was subjected to n-terminal sequence analysis. alignment of the first 30 amino acids revealed that its n-terminal region is virtually identical to that reported for a cysteine-proteinase isolated from the tulahuen strain, including the presence of active site cysteine at position 25. the finding of serine at position 24 of gp57/51 (y strain) has further increased the homology between this ... | 1990 | 1705310 |
| complement-mediated lysis of trypanosoma cruzi trypomastigotes by human anti-alpha-galactosyl antibodies. | antibodies that lyse trypomastigotes in a complement-mediated reaction are believed to be the main participants in the protection against virulent trypanosoma cruzi. antibodies with a specificity for alpha-galactosyl-containing determinants--generally called antigal--were studied to determine their role in the lysis of trypomastigote forms. the titers of antigal markedly increase in chagas's disease. in the present study we demonstrate binding of this antibody to t. cruzi and the complement-medi ... | 1991 | 1706399 |
| the effect of iron deficiency and iron overload on the evolution of chagas disease produced by three strains of trypanosoma cruzi in cfw mice. | 1. cfw mice were fed either on control diet or on iron-deficient diet. 2. after 5 months the mice were infected with cl, y or yuyu strain of trypanosoma cruzi. 3. on the fifth day after the infection, the mice on control diet were divided in three groups: one group remained as controls, two groups were injected either with desferrioxamine or iron-dextran. 4. the severity of the disease was evaluated by parasitemia and mortality. 5. the experimental groups were compared with the infected group fe ... | 1990 | 1710956 |
| the trypanosoma cruzi neuraminidase contains sequences similar to bacterial neuraminidases, ywtd repeats of the low density lipoprotein receptor, and type iii modules of fibronectin. | trypanosoma cruzi expresses a developmentally regulated neuraminidase (tcna) implicated in parasite invasion of cells. we isolated full-length dna clones encoding tcna. sequence analysis demonstrated an open reading frame coding for a polypeptide of 1,162 amino acids. in the n-terminus there is a cysteine-rich domain containing a stretch of 332 amino acids nearly 30% identical to the clostridium perfringens neuraminidase, three repeat motifs highly conserved in bacterial and viral neuraminidases ... | 1991 | 1711561 |
| a novel cell surface trans-sialidase of trypanosoma cruzi generates a stage-specific epitope required for invasion of mammalian cells. | when trypomastigotes of t. cruzi emerge from cells of the mammalian host, they contain little or no sialic acids on their surfaces. however, rapidly upon entering the circulation, they express a unique cell surface trans-sialidase activity. this enzyme specifically transfers alpha (2-3)-linked sialic acid from extrinsic host-derived macromolecules to parasite surface molecules, leading to the assembly of ssp-3, a trypomastigote-specific epitope. the t. cruzi trans-sialidase does not utilize cyti ... | 1991 | 1712251 |
| molecular mimicry by trypanosoma cruzi: the f1-160 epitope that mimics mammalian nerve can be mapped to a 12-amino acid peptide. | antigenic mimicry by trypanosoma cruzi antigens that share epitopes with mammalian tissues may drive autoreactive b- or t-cell clones to expand and cause autoimmune pathogenesis. we have been studying one of these antigens, f1-160, a 160-kda protein on the surface of t. cruzi that antigenically mimics a 48-kda protein found in mammalian axonal and myenteric plexus cells. the f1-160 antigen has been characterized by cloning and expression of t. cruzi dna encoding f1-160 in escherichia coli. recom ... | 1991 | 1712477 |
| chagas' disease: cd5 b-cell-dependent th2 pathology? | 1991 | 1714086 | |
| immunogenicity of trypanosoma cruzi cysteine proteinase. | 1991 | 1714087 | |
| antigens of trypanosoma cruzi that mimic mammalian nervous tissues: investigations of their role in the autoimmune pathophysiology of chronic chagas' disease. | 1991 | 1714088 | |
| molecular mimicry and chagas' heart disease: high anti-r-13 autoantibody levels are markers of severe chagas heart complaint. | 1991 | 1714089 | |
| origin and significance of anti-heart and anti-skeletal muscle autoantibodies in chagas' disease. | 1991 | 1714090 | |
| trypanosoma cruzi: a carbohydrate epitope defined by a monoclonal antibody as a possible marker of the acute phase of human chagas' disease. | an igm monoclonal antibody (mab) against a carbohydrate epitope present in trypanosoma cruzi trypomastigote excretory-secretory antigens and expressed by different developmental stages of the parasite (epimastigote, trypomastigote and intracellular amastigote) was linked to a solid phase matrix and used as an antigen-capture antibody. human serum complexes containing the epitope were then detected by using specific secondary antibodies against human immunoglobulin isotypes. results of detection ... | 1991 | 1715133 |
| characterization of a trypanosoma cruzi c3 binding protein with functional and genetic similarities to the human complement regulatory protein, decay-accelerating factor. | evasion of the complement system by microorganisms is an essential event in the establishment of infection. in the case of trypanosoma cruzi, the causative agent of chagas disease, resistance to complement-mediated lysis is a developmentally regulated characteristic. infectious trypomastigotes are resistant to complement-mediated lysis in the absence of immune antibodies, whereas the insect forms (epimastigotes) are sensitive to lysis via the alternative complement pathway. we have purified a de ... | 1991 | 1717552 |
| carbohydrate epitopes are responsible for antibody cross-reactivity in trypanosoma cruzi-infected mice. | anti-trypanosoma cruzi antibodies can be eluted from western blots of t. cruzi antigens and thereby are fractionated on the basis of the electrophoretic mobility of the antigens to which they bind. antibodies fractionated by these methods can bind antigens with electrophoretic mobility different from those antigens from which they are eluted. such antibodies thus are considered cross-reactive. studies in which the target antigens are reacted with sodium periodate to destroy carbohydrate epitopes ... | 1991 | 1717672 |
| nucleotide sequence and transcription of a trypomastigote surface antigen gene of trypanosoma cruzi. | in previous studies we identified a 500-bp segment of the gene, tsa-1, which encodes an 85-kda trypomastigote-specific surface antigen of the peru strain of trypanosoma cruzi. tsa-1 was shown to be located at a telomeric site and to contain a 27-bp tandem repeat unit within the coding region. this repeat unit defines a discrete subset of a multigene family and places the tsa-1 gene within this subset. in this study, we present the complete nucleotide sequence of the tsa-1 gene from the peru stra ... | 1991 | 1717846 |
| direct lysis of trypanosoma cruzi: a novel effector mechanism of protection mediated by human anti-gal antibodies. | anti-gal antibodies directed against a carbohydrate epitope present in mouse laminin (galactosyl alpha 1-3 galactose) and detected in high levels in sera from patients in the acute phase of chagas disease are responsible for the direct lysis (dl) of trypanosoma cruzi blood forms independent of either the classic or alternative complement pathways. furthermore, the lectins euonymus europaeus (ee) specific for the carbohydrates gal alpha 1-3 gal present a similar lytic activity against t. cruzi at ... | 1991 | 1717927 |
| a new member of a family of site-specific retrotransposons is present in the spliced leader rna genes of trypanosoma cruzi. | a new member of a family of site-specific retrotransposons is described in the new world trypanosome trypanosoma cruzi. this element, czar (cruzi-associated retrotransposon), resembles two previously described retrotransposons found in the african trypanosome t. brucei gambiense and the mosquito trypanosomatid crithidia fasciculata in specifically inserting between nucleotides 11 and 12 of the highly conserved 39-mer of the spliced leader rna (sl-rna) gene. czar is similar in overall organizatio ... | 1991 | 1719380 |
| antibodies to mouse laminin in patients with systemic sclerosis (scleroderma) recognize galactosyl (alpha 1-3)-galactose epitopes. | employing radioimmunoinhibition assays with distinct oligosaccharides as inhibitors, this study demonstrates that the epitope recognized on mouse laminin by sera from patients with systemic sclerosis (scleroderma) is a terminal galactosyl (alpha 1-3)-galactose disaccharide. the reaction with this alpha-digalactose was further confirmed when the sera were tested in radioimmunoassay (ria) binding assay and in elisa with synthetic galactose alpha 1-3 galactose coupled to human serum albumin. the ci ... | 1991 | 1721011 |
| igg isotype profiles induced in mice by two trypanosoma cruzi electronegative antigens. | in this work we studied the igg isotypes induced in mice immunized with two trypanosoma cruzi acidic antigenic fractions (f iv and eas 4.5) and the level of protection to a later infection with parasites. f iv is a cytosolic antigen from epimastigotes, and eas 4.5 is an exoantigen released by trypomastigotes. the most relevant epitopes of eas 4.5 are carbohydrates. a high prevalence of igg1, low levels of igg3 and no igg2 antibodies against f iv and eas 4.5 were found in sera obtained 2 weeks af ... | 1991 | 1721610 |
| relationship between dna methylation and cell proliferation in trypanosoma cruzi. | 5-azacytidine treatment of t. cruzi epimastigotes in culture induces active cell proliferation. this effect was detected as an increase in the cell number and [3h-methyl]thymidine incorporation into dna. 5-azacytidine does not alter other metabolic parameters. we have previously demonstrated that 5-azacytidine induces dna hypomethylation in t. cruzi. accordingly, we suggest that this chemical modification may be related to the control of t. cruzi cell division. | 1991 | 1722465 |
| recovery of triatoma infestans populations after insecticide application: an experimental field study. | the capacity of populations of triatoma infestans (klug) (hemiptera: reduviidae) to survive and recover was assessed after application of insecticide (gamma-hch at a rate of 0.5 g a.i./m2) at different seasons. t. infestans populations were maintained in experimental chicken houses under natural climatic conditions in a region of argentina endemic for chagas disease transmitted by these bugs. based on previous studies of t. infestans populations in these habitats, each experimental group was set ... | 1991 | 1722730 |
| assembly of macromolecular pores by immune defense systems. | immune defence systems (complement, cytolytic lymphocytes) make use of transmembrane pores assembled from up to 20 soluble monomers in a highly regulated process to induce cell death. inhibitors of pore formation have been found which protect blood, endothelial and epithelial cells from the destructive effect of complement lesions. recently, a pore-forming protein showing immunological crossreactivity to complement c9 has been found in the protozoan parasite trypanosoma cruzi, thereby extending ... | 1991 | 1722985 |
| patients infected with leishmania donovani chagasi can have antibodies that recognize heat shock and acidic ribosomal proteins of trypanosoma cruzi. | 1991 | 1723150 | |
| african trypanosomes express an immunogenic protein with a repeating epitope of 24 amino acids. | infection with intracellular protozoan parasites such as plasmodium, leishmania and trypanosoma cruzi induces a strong antibody response against proteins containing tandem repeats, suggesting that these repetitive epitopes may camouflage vulnerable parasite antigens from a 'protective' immune response. we tested this theory by immunoscreening a cdna expression library of african trypanosomes, extracellular parasites that evade their hosts' immune response by antigenic variation, and found that t ... | 1991 | 1723507 |
| immunocytochemical identification of leishmania and trypanosoma cruzi amastigotes in situ with homologous and heterologous polyclonal antibodies. | the unlabelled antibody peroxidase-antiperoxidase method was used to study the immunocytochemical properties of leishmania and trypanosoma cruzi amastigotes in situ after tissues had been submitted to different fixation procedures. antisera were obtained from rabbits chronically infected with different strains of t. cruzi or immunized with l. mexicana amazonensis and l. braziliensis guyanensis, and were applied on 5 microns thick sections. t. cruzi antigens were well stained by the three anti-t. ... | 1991 | 1726128 |
| [spontaneous variability of ventricular extrasystole in chronic chagas cardiopathy]. | to study the spontaneous variability of single (vpcs) and coupled (cvpcs) in patients with chronic chagas' disease (ccd). | 1991 | 1726676 |
| antibodies to t. cruzi cytosol acidic antigens (fiv) in chagas' disease recognize parasite cell surface and human heart epitopes. | the expression of t. cruzi electronegative antigens (fiv) on the parasite surface and their cross-reactivity with heart tissue antigens was studied. for the former purpose epimastigotes (epi) treated with glutaraldehyde were used to absorb antibodies against surface antigens. glutaraldehyde fixed heart tissue was used for absorption of antibodies in sera from two groups of chagasic patients with normal and altered electrocardiogram. the absorption of sera from normal electrocardiogram group with ... | 1990 | 1726727 |
| the influence of humidity on the residual action of benzene hexachloride (bhc). | in controlled humidity chambers in the laboratory differences in the absorption velocity of bhc were observed depending on the substrate sprayed. while bhc is no longer used in chagas' disease control this data could have relevance to spraying houses in a control programme with other insecticides. | 2013 | 1726759 |