Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| gramene, a tool for grass genomics. | gramene (http://www.gramene.org) is a comparative genome mapping database for grasses and a community resource for rice (oryza sativa). it combines a semi-automatically generated database of cereal genomic and expressed sequence tag sequences, genetic maps, map relations, and publications, with a curated database of rice mutants (genes and alleles), molecular markers, and proteins. gramene curators read and extract detailed information from published sources, summarize that information in a stru ... | 2002 | 12481044 |
| targeted analysis of orthologous phytochrome a regions of the sorghum, maize, and rice genomes using comparative gene-island sequencing. | a "gene-island" sequencing strategy has been developed that expedites the targeted acquisition of orthologous gene sequences from related species for comparative genome analysis. a 152-kb bacterial artificial chromosome (bac) clone from sorghum (sorghum bicolor) encoding phytochrome a (phya) was fully sequenced, revealing 16 open reading frames with a gene density similar to many regions of the rice (oryza sativa) genome. the sequences of genes in the orthologous region of the maize (zea mays) a ... | 2002 | 12481045 |
| t-dna insertional mutagenesis for activation tagging in rice. | we have developed a new t-dna vector, pga2715, which can be used for promoter trapping and activation tagging of rice (oryza sativa) genes. the binary vector contains the promoterless beta-glucuronidase (gus) reporter gene next to the right border. in addition, the multimerized transcriptional enhancers from the cauliflower mosaic virus 35s promoter are located next to the left border. a total of 13,450 t-dna insertional lines have been generated using pga2715. histochemical gus assays have reve ... | 2002 | 12481047 |
| dasheng and rire2. a nonautonomous long terminal repeat element and its putative autonomous partner in the rice genome. | dasheng is one of the highest copy number long terminal repeat elements and one of the most recent elements to amplify in the rice (oryza sativa) genome. however, the absence of any significant coding capacity for retroviral proteins, including gag and pol, suggests that dasheng is a nonautonomous element. here, we have exploited the availability of 360 mb of rice genomic sequence to identify a candidate autonomous element. rire2 is a previously described gypsy-like long terminal repeat retrotra ... | 2002 | 12481052 |
| acclimation of rice photosynthesis to irradiance under field conditions. | acclimation to irradiance was measured in terms of light-saturated photosynthetic carbon assimilation rates (p(max)), rubisco, and pigment content in mature field-grown rice (oryza sativa) plants in tropical conditions. measurements were made at different positions within the canopy alongside irradiance and daylight spectra. these data were compared with a second experiment in which acclimation to irradiance was assessed in uppermost leaves within whole-plant shading regimes (10% low light [ll], ... | 2002 | 12481083 |
| a rice mutant defective in si uptake. | rice (oryza sativa) accumulates silicon (si) in the tops to levels up to 10.0% of shoot dry weight, but the mechanism responsible for high si uptake by rice roots is not understood. we isolated a rice mutant (gr1) that is defective in active si uptake by screening m(2) seeds (64,000) of rice cv oochikara that were treated with 10(-3) m sodium azide for 6 h at 25 degrees c. there were no phenotypic differences between wild type (wt) and gr1 except that the leaf blade of gr1 remained droopy when s ... | 2002 | 12481095 |
| introduction of a na+/h+ antiporter gene from atriplex gmelini confers salt tolerance to rice. | we engineered a salt-sensitive rice cultivar (oryza sativa cv. kinuhikari) to express a vacuolar-type na+/h+ antiporter gene from a halophytic plant, atriplex gmelini (agnhx1). the activity of the vacuolar-type na+/h+ antiporter in the transgenic rice plants was eight-fold higher than that in wild-type rice plants. salt tolerance assays followed by non-stress treatments showed that the transgenic plants overexpressing agnhx1 could survive under conditions of 300 mm nacl for 3 days while the wild ... | 2002 | 12482579 |
| metabolism of homocysteine-thiolactone in plants. | editing of the amino acid homocysteine (hcy) by certain aminoacyl-trna synthetases results in the formation of an intramolecular thioester, hcy-thiolactone. here we show that the plant yellow lupin, lupinus luteus, has the ability to synthesize hcy-thiolactone. the inhibition of methylation of hcy to methionine by the anitifolate drug aminopterin results in greatly enhanced synthesis of hcy-thiolactone by l. luteus plants. methionine inhibits the synthesis of hcy-thiolactone in l. luteus, sugges ... | 2003 | 12496251 |
| novel rice map kinases osmsrmk3 and oswjumk1 involved in encountering diverse environmental stresses and developmental regulation. | we report isolation of two novel rice (oryza sativa l.) mitogen-activated protein kinases (mapks), osmsrmk3 (multiple stress responsive) and oswjumk1 (wound- and ja-uninducible) that most likely exist as single copy genes in its genome. osmsrmk3 and oswjumk1 encode 369 and 569 amino acid polypeptides having the mapk family signature and phosphorylation activation motifs tey and tdy, respectively. steady state mrna analyses of these mapks with constitutive expression in leaves of two-week-old see ... | 2003 | 12507518 |
| sugar modulation of alpha-amylase genes under anoxia. | tolerance to low oxygen availability is likely to be due to the interaction of several factors. sugar availability is one of the elements required to support anaerobic metabolism. in cereal grains the availability of soluble sugars is limited, while starch is stored in large amounts. degradation of starch under anoxia is therefore needed to avoid sugar starvation leading to rapid cell death. the striking difference in the ability to produce alpha-amylase when comparing the anoxia-tolerant rice ( ... | 0 | 12509335 |
| regulation of submergence-induced enhanced shoot elongation in oryza sativa l. | rice (oryza sativa l.) is the only cereal that can be cultivated in the frequently flooded river deltas of south-east and south asia. the survival strategies used by rice have been studied quite extensively and the role of several phytohormones in the elongation response has been established. deep-water rice cultivars can diminish flooding stress by rapid elongation of their submerged tissues to keep up with the rising waters. other rice cultivars may react by mechanisms of submergence tolerance ... | 2003 | 12509346 |
| responses by coleoptiles of intact rice seedlings to anoxia: k(+) net uptake from the external solution and translocation from the caryopses. | this study evaluated the effects of anoxia on k(+) uptake and translocation in 3-4-d-old, intact, rice seedlings (oryza sativa l. cv. calrose). rates of net k(+) uptake from the medium over 24 h by coleoptiles of anoxic seedlings were inhibited by 83-91 %, when compared with rates in aerated seedlings. similar uptake rates, and degree of inhibition due to anoxia, were found for rb(+) when supplied over 1.5-2 h, starting 22 h after imposing anoxia. the rb(+) uptake indicated that intact coleoptil ... | 2003 | 12509347 |
| aerenchyma and an inducible barrier to radial oxygen loss facilitate root aeration in upland, paddy and deep-water rice (oryza sativa l.). | the present study evaluated waterlogging tolerance, root porosity and radial o(2) loss (rol) from the adventitious roots, of seven upland, three paddy, and two deep-water genotypes of rice (oryza sativa l.). upland types, with the exception of one genotype, were as tolerant of 30 d soil waterlogging as the paddy and deep-water types. in all but one of the 12 genotypes, the number of adventitious roots per stem increased for plants grown in waterlogged, compared with drained, soil. when grown in ... | 2003 | 12509350 |
| polyphyletic origin of cultivated rice: based on the interspersion pattern of sines. | the wild rice species oryza rufipogon with wide intraspecific variation is thought to be the progenitor of the cultivated rice species oryza sativa with two ecotypes, japonica and indica. to determine the origin of cultivated rice, subfamily members of the rice retroposon p-sine1, which show insertion polymorphism in the o. sativa -o. rufipogon population, were identified and used to "bar code" each of 101 cultivated and wild rice strains based on the presence or absence of the p-sine1 members a ... | 2003 | 12519908 |
| mosdb: an integrated information resource for rice genomics. | the mips rice (oryza sativa) database (mosdb; http://mips.gsf.de/proj/rice) provides a comprehensive data collection dedicated to the genome information of rice. rice (o. sativa l.) is one of the most important food crops for over half the world's population and serves as a major model system in cereal genome research. mosdb integrates data from two publicly available rice genomic sequences, o. sativa l. ssp. indica and o. sativa l. ssp. japonica. besides regularly updated rice genome sequence i ... | 2003 | 12519979 |
| the tigr rice genome annotation resource: annotating the rice genome and creating resources for plant biologists. | rice is not only a major food staple for the world's population but it also is a model species for a major group of flowering plants, the monocotyledonous plants. draft genomic sequence of two subspecies of rice, oryza sativa spp. japonica and indica ssp. are publicly available. to provide the community with a resource to data-mine the rice genome, we have constructed an annotation resource for rice (http://www.tigr.org/tdb/e2k1/osa1/). in this resource, we have annotated the rice genome for gen ... | 2003 | 12519988 |
| an active dna transposon family in rice. | the publication of draft sequences for the two subspecies of oryza sativa (rice), japonica (cv. nipponbare) and indica (cv. 93-11), provides a unique opportunity to study the dynamics of transposable elements in this important crop plant. here we report the use of these sequences in a computational approach to identify the first active dna transposons from rice and the first active miniature inverted-repeat transposable element (mite) from any organism. a sequence classified as a tourist-like mi ... | 2003 | 12520302 |
| the plant mite mping is mobilized in anther culture. | transposable elements constitute a large portion of eukaryotic genomes and contribute to their evolution and diversification. miniature inverted-repeat transposable elements (mites) constitute one of the main groups of transposable elements and are distributed ubiquitously in the genomes of plants and animals such as maize, rice, arabidopsis, human, insect and nematode. because active mites have not been identified, the transposition mechanism of mites and their accumulation in eukaryotic genome ... | 2003 | 12520303 |
| mobilization of a transposon in the rice genome. | rice (oryza sativa l.) is an important crop worldwide and, with the availability of the draft sequence, a useful model for analysing the genome structure of grasses. to practice efficient rice breeding through genetic engineering techniques, it is important to identify the economically important genes in this crop. the use of mobile transposons as gene tags in intact plants is a powerful tool for functional analysis because transposon insertions often inactivate genes. here we identify an active ... | 2003 | 12520304 |
| a host-specific bacteria-to-plant signal molecule (nod factor) enhances germination and early growth of diverse crop plants. | lipo-chitooligosaccharides (lcos), or nod factors, are host-specific bacteria-to-plant signal molecules essential for the establishment of a successful n(2)-fixing legume-rhizobia symbiosis. at submicromolar concentrations nod factors induce physiological changes in host and non-host plants. here we show that the nod factor nod bj v(c18:1,mefuc) of bradyrhizobium japonicum 532c enhances germination of a variety of economically important plants belonging to diverse botanical families: zea mays, o ... | 2003 | 12520335 |
| chalcone isomerase gene from rice (oryza sativa) and barley (hordeum vulgare): physical, genetic and mutation mapping. | the barley and rice chalcone flavonone isomerase (cfi) genes were isolated and identified by homology to the maize cfi gene. structure analysis indicated high similarity except that the barley gene lacked intron 3. the maize cfi gene has been mapped to three loci, but only a single locus was detected in barley and rice. this explains the lack of observed mutants in maize while a single locus anthocyanin-less 30 (ant30), with four alleles ant30-245, ant30-310, ant30-272 and ant30-287 has been des ... | 2003 | 12527208 |
| sequence analysis, transcriptional and posttranscriptional regulation of the rice vdac family. | the voltage-dependent anion-selective channel (vdac) is a mitochondrial outer membrane ion channel. different isoforms exist in plants but information about their specific role remains to be established. our purpose is to find out the structural features common to three rice vdac isoforms and to investigate their (post)transcriptional regulation in response to an osmotic stress. two new cdnas encoding mitochondrial vdac from rice (oryza sativa) were isolated, sequenced and characterized: a phylo ... | 2003 | 12527425 |
| putative proteins related to group ii intron reverse transcriptase/maturases are encoded by nuclear genes in higher plants. | the arabidopsis thaliana nuclear genome sequence revealed several open reading frames encoding proteins related to group ii intron-encoded reverse transcriptase/maturases. here, we show via sequence alignments that at least four such open reading frames are conserved in the nuclear genomes of a.thaliana and oryza sativa (rice) and that they encode putative proteins belonging to two different classes (nmat-1 and nmat-2), neither of which is associated with a group ii intron rna structure. the two ... | 2003 | 12527773 |
| rapid determination of rice seed vigour by spontaneous chemiluminescence and singlet oxygen generation during early imbibition. | using a highly sensitive single photon counter, a spontaneous chemiluminescence (cl) study on rice (oryza sativa l.) seeds stored in different years was carried out. we first observed that the degree of ageing in rice seeds was related to the intensity of spontaneous cl during early imbibition (0-30 min). rice seeds stored for a shorter time had a stronger intensity of cl in early imbibition. the germination rate of rice seeds showed an obvious positive correlation with the intensity of spontane ... | 2015 | 12536375 |
| [ultrastructural localization of atpase activity in fertile and sterile anther of rice (oryza sativa l. cv. marxie)]. | pb3 (po4)2-precipitation was used to study the atpase activities both in fertile and sterile anthers of rice (oryza sativa l. cv. marxie). at big-vacuole pollen stage, tapetum of the fertile anthers showed high atpase activity in their nuclei. in fertile pollen, atpase was localized on the outside surface of the exine and in the nucleus both at big-vacuole and at bi-nucleate pollen stage. at late bi-nucleate pollen stage, a large amount of pb3 (po4)2 precipitated in endintine of the fertile poll ... | 2001 | 12549213 |
| [effects of free-air co2 enrichment (face) on yield formation in rice (oryza sativa)]. | effect of free-air co2 enrichment (face, 200 mumol.mol-1 higher than ck) on rice yield and its components under field condition were studied with a japonica variety 99-15. results showed that face treatment had no obvious effect on plant height and number of leaves on main stem, but could quicken growth process of rice, shorten the whole growth duration. increment of nitrogen application rate could weaken the effect of face on whole growth duration. the number of tillers and panicles, and fulfil ... | 2002 | 12557661 |
| [effect of free-air co2 enrichment (face) on spikelets differentiation and retrogression in rice (oryza sativa)]. | effect of free-air co2 enrichment (face), 200 mumol.mol-1co2 elevation under field condition, on the number of differentiated, retrograded, and survived primary and secondary branches and their spikelets in rice was investigated, and the percentage of retrograded branches and spikelets under face treatment were also calculated. results showed that the number of differentiated primary and secondary branches, the number and percentage of retrograded primary branches per panicle were not significan ... | 2002 | 12557662 |
| [effect of free-air co2 enrichment (face) on nitrogen accumulation and utilization efficiency in rice (oryza sativa)]. | effect of face, 200 mumol.mol-1 co2 elevation under field condition in this study, on nitrogen accumulation and nitrogen use efficiency in rice was investigated. results showed that nitrogen content (%) in rice plant sampled at different growth stages decreased significantly under face treatment, but face had no obvious effect on nitrogen accumulation in rice plant due to the significant enhancement in dry matter production. face treatment resulted in the significant increase in nitrogen use eff ... | 2002 | 12557663 |
| [responses of rice (oryza sativa) growth and its c, n and p composition to face (free-air carbon dioxide enrichment) and n, p fertilization]. | face (free-air carbon dioxide enrichment) was used to study the effects of elevated co2 on rice (oryza sativa) growth, tissue c/n, n and p concentration and uptake at different development stages under two n and two p levels. results showed that elevated co2 increased dry matter accumulation in rice stem, ear and root. leaf dry matter was increased at tillering stage and no significant effect was found at jointing, heading and ripening stages. n concentration of stem and leaf was decreased. ear ... | 2002 | 12557664 |
| [effect of face on competition between a c3 crop (rice, oryza sativa) and a c4 weed (barnyardgrass, echinochloa crusgalli)]. | the growth and competition of a c3 crop rice and a c4 weed, barnyardgrass under face(free-air carbon dioxide enrichment) conditions were studied by field experiment. the results showed that under face condition, the biomass and yield, leaf number, tiller number, and leaf area index of rice increased, while those of barnyardgrass decreased. both of the leaf area indexed of rice and barnyardgrass were decreased, while their net assimilation rate increased. when the ratio of planting density of ric ... | 2002 | 12557665 |
| effects of elevated atmospheric co2 on nematode trophic groups in a chinese paddy-field ecosystem. | soil fauna plays significant roles in the detritus food webs of agroecosystems, they are the essential contributors to the decomposition of soil organic matter, mineralization of plant nutrients and nutrient cycling. some evidences indicate that soil fauna of the detritus food webs appears to show positive, neutral or negative responses to global change, especially atmospheric co2 enrichment across different studies. soil nematodes are representative of a large portion of this fauna, since they ... | 2002 | 12557673 |
| [effects of enhanced ultraviolet-b radiation on growth development and yield formation in rice (oryza sativa l.)]. | effect of enhanced ultraviolet-b radiation (280-320 nm) on growth, development and yield formation in the three rice cultivars (oryza sativa l.) under pot conditions were investigated. the results showed that enhanced ultraviolet-b radiation decreased rice height, tiller number, total leaf area and biomass, but the inhibitory rate varied in different cultivars and developmental stages. plant height was decreased by 9.4%-12.2% in seedling stage, plant shoot and root of dry matter were decreased b ... | 2002 | 12557675 |
| [responses of agricultural crops of free-air co2 enrichment]. | over the past decade, free-air co2 enrichment (face) experiments have been conducted on several agricultural crops: wheat(triticum aestivum l.), perennial ryegrass (lolium perenne), and rice(oryza sativa l.) which are c3 grasses; sorghum (sorghum bicolor (l.) möench), a c4 grass; white clover (trifolium repens), a c3 legume; potato (solanum tuberosum l.), a c3 forb with tuber storage; and cotton (gossypium hirsutum l.) and grape (vitis vinifera l.) which are c3 woody perennials. using reports fr ... | 2002 | 12557686 |
| sucrose-phosphatase gene families in plants. | sucrose-phosphatase (spp; ec 3.1.3.24) catalyzes the final step in the pathway of sucrose biosynthesis and higher plants contain multiple isoforms of the enzyme encoded by different genes. the genome of the dicotyledonous plant arabidopsis thaliana (thale cress) contains four spp-like genes on chromosomes 1 (atspp1), 2 (atspp2) and 3 (atspp3a and atspp3b), all of which are expressed. the genome of the monocotyledonous plant rice (oryza sativa) also contains four spp-like genes, which have very s ... | 2003 | 12559580 |
| molecular cloning and mrna expression analysis of a novel rice (oryzasativa l.) mapk kinase kinase, osedr1, an ortholog of arabidopsis atedr1, reveal its role in defense/stress signalling pathways and development. | mitogen-activated protein kinase (mapk) cascade(s) is important for plant defense/stress responses. though mapks have been identified and characterized in rice (oryza sativa l.), a monocot cereal crop research model, the first upstream component of the kinase cascade, namely mapk kinase kinase (mapkkk) has not yet been identified. here we report the cloning of a novel rice gene encoding a mapkkk, osedr1, designated based on its homology with the arabidopsis mapkkk, atedr1. osedr1, a single copy ... | 2003 | 12559953 |
| arsenic contamination of bangladesh paddy field soils: implications for rice contribution to arsenic consumption. | arsenic contaminated groundwater is used extensively in bangladesh to irrigate the staple food of the region, paddy rice (oryza sativa l.). to determine if this irrigation has led to a buildup of arsenic levels in paddy fields, and the consequences for arsenic exposure through rice ingestion, a survey of arsenic levels in paddy soils and rice grain was undertaken. survey of paddy soils throughout bangladesh showed that arsenic levels were elevated in zones where arsenic in groundwater used for i ... | 2003 | 12564892 |
| regulation of biosynthesis and intracellular localization of rice and tobacco homologues of nucleosome assembly protein 1. | the nucleosome assembly protein 1 (nap1) is considered to be a conserved histone chaperone, facilitating the assembly of nucleosomes in all eukaryotes. however, studies in yeast and animal cells also indicated that nap1 proteins have diverse functions likely independent of nucleosome-assembly activity. here, we describe the isolation and characterization of cdnas encoding nap1-like proteins from the monocotyledon rice ( oryza sativa l.) and the dicotyledon tobacco ( nicotiana tabacum l.). northe ... | 2003 | 12569397 |
| expression and localization of human lysozyme in the endosperm of transgenic rice. | in order to understand the characteristics of recombinant protein expression and sublocalization in rice ( oryza sativa l.) endosperm, we examined the expression level of human lysozyme protein and its subcellular location in transgenic rice seeds driven by rice glutelin and globulin promoters and signal peptides. a time course of human lysozyme expression during endosperm development was analyzed. the results showed that the expression profile of recombinant protein accumulation in endosperm pa ... | 2003 | 12569401 |
| seed yield and plant biomass increases in rice are conferred by deregulation of endosperm adp-glucose pyrophosphorylase. | in this work we test the hypothesis that yield of rice ( oryza sativa l.) can be enhanced by increasing endosperm activity of adp-glucose pyrophosphorylase (agp), a key enzyme in starch biosynthesis. the potential for increases in yield exist because rice initiates more seeds than are taken to maturity and possesses excess photosynthetic capacity that could be utilized if there were more demand for assimilate. following an approach already shown to be successful in wheat, experiments were design ... | 2003 | 12569408 |
| characterization of proteins responsive to gibberellin in the leaf-sheath of rice (oryza sativa l.) seedling using proteome analysis. | in plants gibberellins (gas) are responsible for triggering stem or internodal elongation. to comprehend the molecular basis of internodal elongation in rice, a proteomics approach using differentially displayed proteins on two-dimensional polyacrylamide gel electrophoresis (2-d page) was carried out to identify the proteins expressed during the ga controlled leaf-sheath elongation response. out of 352 protein spots detected on 2-d page, 32 proteins showed modulation in the expression levels in ... | 2003 | 12576669 |
| oxygen stress and adaptation of a semi-aquatic plant: rice ( oryza sativa). | one of the major abiotic stresses that affects plant growth and development is anoxia or hypoxia. rice is a semi-aquatic plant bestowed with the capability of overcoming oxygen limitation for a considerable period of time. for instance, it can withstand submergence stress either by inherent metabolic adaptations (resistant type), or by keeping its leaves above the water surface by continuously elongating the stem (avoiding type). in the former case, an interplay of several metabolic pathways eng ... | 2002 | 12579355 |
| accelerated shoot overgrowth of rice mutant ao-1 is epistatic to gibberellin-sensitive and -insensitive dwarf mutants. | a shoot overgrowth mutant of rice ( oryza sativa l.), accelerated internode overgrowth-1 ( ao-1), is marked by accelerated longitudinal elongation of aerial parts and overgrowth of internodes at the vegetative stage. the physiological properties of ao-1 were similar to those of wild plants treated with a saturating level of exogenous gibberellins (gas), except for the internode-overgrowth phenotype, which was not mimicked by ga-treated wild plants. the ao-1 mutant was less sensitive to a ga bios ... | 2002 | 12579369 |
| overexpression of bax inhibitor suppresses the fungal elicitor-induced cell death in rice (oryza sativa l) cells. | treatment of suspension-cultured cells of rice (oryza sativa l.) with cell wall extract of rice blast fungus (magnaporthe grisea) elicits a rapid generation of h2o2, alkalinization of culture medium, and eventual cell death. to elucidate genes involved in these processes, we exploited sage (serial analysis of gene expression) technique for the molecular analysis of cell death in suspension-cultured cells treated with the elicitor. among the downregulated genes in the elicitor-treated cells, a bi ... | 2003 | 12581301 |
| a genetic and physical map of the region containing plastochron1, a heterochronic gene, in rice ( oryza sativa l.). | the rice heterochronic gene plastochron1, pla1, shows shorter plastochron and ectopic expression of the vegetative program during the rice reproductive phase resulting in aberrant panicle formation. a genetic and physical map was constructed to isolate the causal gene for the pla1 syndrome. small-scale mapping was carried out to determine the approximate map position of the pla1 locus, and then a high-resolution genetic map was made for pla1-1, one of the pla1 alleles, using an f(2) population c ... | 2002 | 12582477 |
| rflp mapping of a hordeum bulbosum gene highly expressed in pistils and its relationship to homoeologous loci in other gramineae species. | a cdna sequence (hbc8-2) isolated from pistils of the self-incompatible species hordeum bulbosum was analysed for expression pattern and genetic map location. hbc8-2 was expressed just prior to anthesis in mature pistils, and expression was maintained at a high level throughout anthesis. the same expression pattern was found in self-incompatible rye ( secale cereale), but no expression was detected in the self-compatible cereals wheat ( triticum aestivum) or barley ( hordeum vulgare) at comparab ... | 2002 | 12582529 |
| fluorescent-labeled microsatellite panels useful for detecting allelic diversity in cultivated rice ( oryza sativa l.). | four multiplex panels of fluorescent-labeled rice microsatellite markers were designed to survey allelic diversity at a total of 27 simple sequence repeat loci. automated fragment detection, size calling and binning were used to identify the microsatellite alleles. the panels were tested on 72 accessions representing the diversity of ecotypes and isozyme groups in cultivated rice ( oryza sativa l.). genetic diversity was evaluated by estimating the distribution and range of allele sizes, as well ... | 2002 | 12582550 |
| molecular cloning, characterization, expression and chromosomal location of osgapdh, a submergence responsive gene in rice ( oryza sativa l.). | differential clones from submergence stress and control treatment from rice seedlings were isolated by the differential screening method. one of the clones, osgapdh, represented a gene that was expressed at high level during 12-h submergence. a homology search of genbank databases showed that osgapdh had significant sequence homology with maize non-reversible glyceraldehyde-3-phosphate dehydrogenase. the osgapdh sequence consists of 1,772 bp with the longest open reading frame encoding 499 amino ... | 2002 | 12582559 |
| qtl clusters reflect character associations in wild and cultivated rice. | the genetic basis of character association related to differentiation found in the primary gene pool of rice was investigated based on the genomic distribution of quantitative trait loci (qtls). major evolutionary trends in cultivated rice of asiatic origin ( oryza sativa) and its wild progenitor ( o. rufipogon) are: (1) differentiation from wild to domesticated types (domestication), (2) ecotype differentiation between the perennial and annual types in wild races, and (3) the indica versus japo ... | 2002 | 12582574 |
| genetic differentiation for nuclear, mitochondrial and chloroplast genomes in common wild rice ( oryza rufipogon griff.) and cultivated rice ( oryza sativa l.). | the genetic differentiation of nuclear, mitochondrial (mt) and chloroplast (cp) genomes was investigated by southern and pcr analysis using 75 varieties of cultivated rice ( oryza sativa l.) and 118 strains of common wild rice (cwr, oryza rufipogon griff.) from ten countries of asia. the distinguishing differences between the indica and japonica cultivars were detected both in the nuclear genome and the cytoplasmic genome, confirming that the indica-japonica differentiation is of major importanc ... | 2002 | 12582589 |
| mapping quantitative trait loci controlling seed longevity in rice ( oryza sativa l.). | quantitative trait loci (qtls) controlling seed longevity in rice were identified using 98 backcross inbred lines (bils) derived from a cross between a japonica variety nipponbare and an indica variety kasalath. seeds of each bil were kept for 12 months at 30 degrees c in dry conditions to promote loss of viability. to measure seed longevity, we performed an additional aging-processing treatment for 2 months at 30 degrees c maintaining seeds at 15% moisture content. we measured the germination p ... | 2002 | 12582603 |
| qtl mapping and introgression of yield-related traits from oryza glumaepatula to cultivated rice ( oryza sativa) using microsatellite markers. | rice ( oryza sativa) cultivar development currently faces the task of overcoming yield plateaus, which is difficult due to the narrow genetic base of breeding programs. oryza glumaepatula is a diploid wild relative of cultivated rice, native to central and south america, and is therefore a potential source of alleles of agronomic importance to rice breeding programs. we studied 11 agronomic traits in bc(2)f(2) families of the interspecific cross oryza sativa x o. glumaepatula. transgressive line ... | 2002 | 12582630 |
| mapping qtls for root morphology of a rice population adapted to rainfed lowland conditions. | in the rainfed lowlands, rice ( oryza sativa l.) develops roots under anaerobic soil conditions with ponded water, prior to exposure to water stress and aerobic soil conditions that arise later in the season. constitutive root system development in anaerobic soil conditions has been reported to have a positive effect on subsequent expression of adaptive root traits and water extraction during progressive water stress in aerobic soil conditions. we examined quantitative trait loci (qtls) for cons ... | 2002 | 12582650 |
| analysis of the genetic behavior of some starch properties in indica rice ( oryza sativa l.): thermal properties, gel texture, swelling volume. | starch comprises about 90% of milled rice, so that the eating and cooking quality of rice is mainly affected by the starch properties. in the present paper, we analyzed the genetical behavior of gelatinization temperature tested by differential scanning calorimetry (dsc), gel texture, and the swelling volume (sv) of indica rice with an incomplete cross of 4x8 parents. a genetic model which can dissect the effects of triploid seed, the cytoplasm, and the maternal plant on the endosperm traits was ... | 2002 | 12582713 |
| impact of genomics approaches on plant genetics and physiology. | comprehensive analysis of genetic information in higher plants is under way for several plants of biological and agronomical importance. among them, arabidopsis thaliana, a member of brassica family, and oryza sativa(rice) have been chosen as model plants most suitable for genome analysis. sequencing of the genome of a. thaliana was completed in december 2000, and rice genome sequencing is in progress. the accumulated genome sequences, together with the hundreds of thousands of ests from several ... | 2002 | 12582730 |
| genetic differentiation of wild relatives of rice as assessed by rflp analysis. | to study genetic diversity and relationships of wild relatives of rice, 58 accessions of oryza rufipogon, oryza nivara, oryza sativa f. spontanea and the cultivated oryza sativa, representing a wide range of their distribution, were analyzed using the restriction fragment length polymorphism (rflp) technique. all 30-used rflp probes detected polymorphisms among the oryza accessions, with an average of 3.8 polymorphic fragments per probe. considerable genetic diversity was scored among the oryza ... | 2002 | 12582876 |
| overexpression of the wasabi defensin gene confers enhanced resistance to blast fungus ( magnaporthe grisea) in transgenic rice. | transgenic rice ( oryza sativa cv. sasanishiki) overexpressing the wasabi defensin gene, a plant defensin effective against the rice blast fungus, was generated by agrobacterium tumefaciens-mediated transformation. twenty-two t2 homozygous lines harboring the wasabi defensin gene were challenged by the blast fungus. transformants exhibited resistance to rice blast at various levels. the inheritance of the resistance over generations was investigated. t3 plants derived from two highly blast-resis ... | 2002 | 12582903 |
| microsatellites in starch-synthesizing genes in relation to starch physicochemical properties in waxy rice ( oryza sativa l.). | rice starch is composed of amylose and amylopectin. amylose content, an important determinant of rice starch quality, is primarily controlled by the waxy gene, encoding granule-bound starch synthase (gbss). the starch branching enzyme (sbe) and soluble starch synthase (sss) play major roles in the synthesis of amylopectin. microsatellite polymorphisms in the three genes, the wx gene encoding granule-bound starch synthase i, the sbe gene encoding starch branching enzyme i and the sss gene encodin ... | 2002 | 12582915 |
| genetic basis of hybrid breakdown in a japonica/indica cross of rice, oryza sativa l. | reproductive barriers often arise in hybrid progeny between two varietal groups of asian cultivated rice ( oryza sativa l.), japonica and indica. hybrid breakdown showing poor growth habit, and complete sterility was found in the backcrossed progeny derived from a cross between a japonica variety, asominori, and an indica variety, ir24. we employed rflp analysis in the segregating population to study the genetic basis underlying hybrid breakdown. it was found that the hybrid breakdown is caused ... | 2002 | 12582916 |
| genetic dissection of seed shattering, agronomic, and color traits in american wildrice ( zizania palustris var. interior l.) with a comparative map. | a comparative map of american wildrice ( zizania palustris var. interior l.) was used to identify loci controlling seed shattering, plant height, maturity, tiller number, plant habit, panicle length seed length, and color traits. two to six significant quantitative-trait-loci (qtls, p < 0.05) were detected for each trait evaluated, representing the first trait-mapping in wildrice. the chosen population was designed to emphasize the mapping of loci controlling the shattering trait, which is the m ... | 2002 | 12582936 |
| cmap: the comparative genetic map viewer. | cmap, a www comparative genetic map graphical utility, has a search capability and provides comparison of two genetic maps within or between species with dynamic links to data resources and text lists of the shared loci, running in a relational database environment. currently, maps from three species (maize 'zea mays l.', rice 'oryza sativa l.', and sorghum 'sorghum bicolor l.'), representing over 13,800 distinct loci, are available for comparison at http://www.agron.missouri.edu/cmapdb/cmap.htm ... | 2003 | 12584129 |
| effect of o-phenylenediamine on cu adsorption and desorption in red soil and its uptake by paddy rice (oryza sativa). | a study was carried out of cu adsorption and desorption processes in red soil as affected by o-phenylenediamine (o-pd) in the range 0-80 mg/l. the results indicated that the presence of o-pd enhanced cu adsorption in red soil in weakly acid media, meanwhile, desorption percentage of cu from soil, extracted by 1.0 m mgcl(2), also increased when cu adsorption in soil occurred in the presence of o-pd. the response of paddy rice to cu in red soil shows that cu toxicity was mitigated in the presence ... | 2003 | 12586140 |
| expression of a bifunctional fusion of the escherichia coli genes for trehalose-6-phosphate synthase and trehalose-6-phosphate phosphatase in transgenic rice plants increases trehalose accumulation and abiotic stress tolerance without stunting growth. | trehalose plays an important role in stress tolerance in plants. trehalose-producing, transgenic rice (oryza sativa) plants were generated by the introduction of a gene encoding a bifunctional fusion (tpsp) of the trehalose-6-phosphate (t-6-p) synthase (tps) and t-6-p phosphatase (tpp) of escherichia coli, under the control of the maize (zea mays) ubiquitin promoter (ubi1). the high catalytic efficiency (seo et al., 2000) of the fusion enzyme and the single-gene engineering strategy make this an ... | 2003 | 12586876 |
| esterase as molecular marker for salt tolerance in regenerated plants of rice, oryza sativa l. | esterase variation was studied in plants regenerated from callus cultures of four rice (oryza sativa) varieties, viz. pokkali, which is a moderately salt tolerant variety and three salt sensitive varieties mi 48, annapoorna and jyothi. variation was studied at tillering stage of plants regenerated from callus culture and germinated from seeds. somaclonal variants for salt tolerance could be detected using variation in esterase banding pattern and activity. | 2002 | 12587737 |
| responses of soybean to oxygen deficiency and elevated root-zone carbon dioxide concentration. | root flooding is damaging to the growth of crop plants such as soybean (glycine max l.). field flooding for 3 d often results in leaf chlorosis, defoliation, cessation of growth and plant death. these effects have been widely attributed solely to a lack of oxygen in the root-zone. however, an additional damaging factor may be co(2), which attains levels of 30 % (v/v) of total dissolved gases. accordingly, the effects of root-zone co(2) on oxygen-deficient soybean plants were investigated in hydr ... | 2003 | 12588724 |
| characteristics of floral organs related to reliable self-pollination in rice (oryza sativa l.). | in many varieties of rice, the length of basal pores on the thecae just after anthesis is strongly correlated both with the percentage of florets receiving adequate pollen and with the number of pollen grains deposited on the stigmata and its variation (coefficient of variation). therefore, the size of the basal pores is considered to be an important factor for the reliable self-pollination of rice. we discuss how long basal pores may facilitate self- pollination. | 2003 | 12588727 |
| characterization of a unique genomic clone located 5' upstream of the oshsp16.9b gene on chromosome 1 in rice (oryza sativa l. cv tainung no. 67). | small heat-shock proteins (shsp) are the most abundant heat stress-induced proteins in plants. in rice, there are at least seven members of class-i shsp. a 1.6-kb dna fragment was isolated from the ecori-digested rice genomic library probed with the cdna pts1 encoding a 16.9-kda class-i shsp. this fragment was composed of 365-bp tandem direct repeats (drs) and 441-bp near perfect long terminal inverted repeats (ltirs). the drs contain 123-bp regions with 99% nucleotide identity to the 5' coding ... | 2003 | 12589551 |
| methane and nitrous oxide emissions from an irrigated rice of north india. | upland rice was grown in the kharif season (june-september) under irrigated condition in new delhi, india (28 degree 40'n and 77 degree 12'e) to monitor ch4 and n2o emission, as influenced by fertilizer urea, ammonium sulphate and potassium nitrate alone (at 120 kg ha-1) and mixed with dicyandiamide (dcd), added at 10% of applied n. the experimental soil was a typic ustochrept (inceptisol), clay loam, in which rice (oryza sativa l., var. pusa-169, duration: 120-125 days) was grown and ch4 and n2 ... | 2003 | 12591251 |
| identification and mapping of the qtl for aluminum tolerance introgressed from the new source, oryza rufipogon griff., into indica rice (oryza sativa l.). | this study was conducted to identify and map the quantitative trait locus (qtl) controlling al tolerance in rice using molecular markers. a population of 171 f(6) recombinant inbred lines (rils) derived from the cross of oryza sativa (ir64), the al susceptible parent, and oryza rufipogon, the al tolerant parent, was evaluated for al tolerance using a nutrient solution with and without 40 ppm of active al(+3). a genetic map, consisting of 151 molecular markers covering 1,755 cm with an average di ... | 2003 | 12595985 |
| osralyase1, a putative f-box protein identified in rice, oryza sativa, with enzyme activity identical to that of wheat ralyase. | a rice gene, osralyase1, encoding a product similar to wheat ribosomal rna apurinic site specific lyase (ralyase), was isolated and expressed in vitro. an open reading frame of the gene predicted a protein of 476 amino acid residues with 75% identity to ralyase and contained an f-box-like motif in its amino terminal region. the rice gene product expressed in a wheat-germ protein expression system had the same characteristics as its wheat counterpart, cleaving a specific depurinated site of the 2 ... | 2002 | 12596877 |
| development and mapping of 2240 new ssr markers for rice (oryza sativa l.). | a total of 2414 new di-, tri- and tetra-nucleotide non-redundant ssr primer pairs, representing 2240 unique marker loci, have been developed and experimentally validated for rice (oryza sativa l.). duplicate primer pairs are reported for 7% (174) of the loci. the majority (92%) of primer pairs were developed in regions flanking perfect repeats > or = 24 bp in length. using electronic pcr (e-pcr) to align primer pairs against 3284 publicly sequenced rice bac and pac clones (representing about 83% ... | 2002 | 12597276 |
| development and mapping of 2240 new ssr markers for rice (oryza sativa l.) (supplement). | 2002 | 12597280 | |
| two genes encoding protein phosphatase 2a catalytic subunits are differentially expressed in rice. | type 2a serine/threonine protein phosphatase (pp2a) plays a variety of regulatory roles in metabolism and signal transduction. two closely related pp2a catalytic subunit (pp2ac) genes, ospp2a-1 and ospp2a-3, have been isolated from the monocot oryza sativa. both genes contain six exons and five introns which intervene at identical locations, suggesting they have descended from a recent duplication event. their encoded proteins share 97% sequence identity and are highly similar (94-96%) to a pp2a ... | 2003 | 12602862 |
| ossend-1: a new rad2 nuclease family member in higher plants. | a novel endonuclease, a new member of the rad2 nuclease family, has been identified from the higher plant, rice (oryza sativa l. cv. nipponbare), and designated as ossend-1. the open reading frame of the ossend-1 cdna encoded a predicted product of 641 amino acid residues with a molecular weight of 69.9 kda. the encoded protein showed a relatively high degree of sequence homology with the rad2 nuclease family proteins, especially rad2 nuclease, but it differed markedly from fen-1, xpg or hex1/ex ... | 2003 | 12602891 |
| salinity stress-tolerant and -sensitive rice (oryza sativa l.) regulate akt1-type potassium channel transcripts differently. | in the indica rice (oryza sativa l.) a cdna was characterized that encoded osakt1 homologous to inward-rectifying potassium channels of the akt/kat subfamily. transcript analysis located osakt1 predominantly in roots with low abundance in leaves. cell-specificity of osakt expression was analyzed by in situ hybridizations. in roots, strongest signals were localized to the epidermis and the endodermis, whereas lower transcript levels were detected in cells of the vasculature and the cortex. in lea ... | 2003 | 12602892 |
| characterization of transgenic rice plants over-expressing the stress-inducible beta-glucanase gene gns1. | the gns1 gene of rice (oryza sativa l. japonica) encodes 1,3;1,4-beta glucanase (ec 3.2.1.73), which hydrolyzes 1,3;1,4-beta-glucosidic linkages on 1,3;1,4-beta-glucan, an important component of cell walls in the poaceae family. rna and protein gel blot analyses demonstrated that blast disease or dark treatment induced the expression of the gns1 gene. to assess the function of the gns1 gene in disease resistance, we characterized transgenic rice plants constitutively expressing the gns1 gene. th ... | 2003 | 12602898 |
| rice mapks. | mitogen-activated protein kinase (mapk) cascades are evolutionary conserved from unicellular to complex eukaryotic organisms, and constitute one of the major signalling pathways involved in regulating a wide range of cellular activities from growth and development to cell death. mapks of rice (oryza sativa l.), the most important of all food crops and an established monocot plant research model, have seen considerable progress mainly on their identification and characterization during the past o ... | 2003 | 12604328 |
| osdreb genes in rice, oryza sativa l., encode transcription activators that function in drought-, high-salt- and cold-responsive gene expression. | the transcription factors drebs/cbfs specifically interact with the dehydration-responsive element/c-repeat (dre/crt) cis-acting element (core motif: g/accgac) and control the expression of many stress-inducible genes in arabidopsis. in rice, we isolated five cdnas for dreb homologs: osdreb1a, osdreb1b, osdreb1c, osdreb1d, and osdreb2a. expression of osdreb1a and osdreb1b was induced by cold, whereas expression of osdreb2a was induced by dehydration and high-salt stresses. the osdreb1a and osdre ... | 2003 | 12609047 |
| organ-specific expressions and chromosomal locations of two mitochondrial aldehyde dehydrogenase genes from rice (oryza sativa l.), aldh2a and aldh2b. | recent studies have suggested that mitochondrial aldehyde dehydrogenase (aldehyde:nad(p)(+) oxidoreductase, ec 1.2.1.3) (aldh2) plays essential roles in pollen development in plants. rice (oryza sativa l.) aldh2 is encoded by at least two aldh2 genes, one of which (aldh2a) was previously identified. in this study, to understand the roles of aldh2 in rice, we isolated and characterized a cdna clone encoding another rice aldh2 (aldh2b). an in vitro aldh assay indicated that aldh2b possesses an nad ... | 2003 | 12609740 |
| the promoter of a rice glycine-rich protein gene, osgrp-2, confers vascular-specific expression in transgenic plants. | the genomic sequence of a rice (oryza sativa l.) glycine-rich protein (grp) gene, designated osgrp-2, has been previously determined (genbank u40708). primer extension analysis indicated that transcription starts 47 bp upstream of the translation start codon. to gain an insight into the transcriptional regulation of this gene, the 2,401-bp promoter sequence and a series of its 5' deletions were transcriptionally fused to the beta-glucuronidase (gus) gene. gus activity was subsequently assayed in ... | 2003 | 12624770 |
| [developmental genetic analysis of panicle dry weight of indica hybrid rice oryza sativa under different environmental conditions]. | four indica sterile lines (a) and corresponding maintainer lines (b) as well as five indica restorer lines, which varied in panicle dry weight, were used to create genetic populations of two generations according to incomplete diallel cross design (4 x 5). analysis on panicle dry weight at different developmental stages based on tow-years experimental data was conducted by using additive-dominant developmental genetic models with genotype effects and genotype x environment interaction effects. t ... | 2002 | 12624993 |
| chronopathological aspects of disease incidence in rice (oryza sativa l.). | rice seedlings maintained under uncontrolled glasshouse conditions were inoculated with conidial suspensions of a fungal pathogen, helminthosporium oryzae, at various times during the 24h. significant increase in the percent germination and germ tube length of conidia were observed in the rice samples inoculated at 02:00 and 06:00h. the 24 h temporal variation in leaf temperature was positively correlated with variation in stomatal movements. the results indicate a 24 h rhythm in the behavior of ... | 2003 | 12638692 |
| convergent evolution of perenniality in rice and sorghum. | annual and perennial habit are two major strategies by which grasses adapt to seasonal environmental change, and these distinguish cultivated cereals from their wild relatives. rhizomatousness, a key trait contributing to perenniality, was investigated by using an f(2) population from a cross between cultivated rice (oryza sativa) and its wild relative, oryza longistaminata. molecular mapping based on a complete simple sequence-repeat map revealed two dominant-complementary genes controlling rhi ... | 0 | 12642667 |
| whole-genome comparison of leucine-rich repeat extensins in arabidopsis and rice. a conserved family of cell wall proteins form a vegetative and a reproductive clade. | we have searched the arabidopsis and rice (oryza sativa) genomes for homologs of lrx1, an arabidopsis gene encoding a novel type of cell wall protein containing a leucine-rich repeat (lrr) and an extensin domain. eleven and eight lrx (lrr/extensin) genes have been identified in these two plant species, respectively. the lrx gene family encodes proteins characterized by a short n-terminal domain, a domain with 10 lrrs, a cysteine-rich motif, and a variable c-terminal extensin-like domain. phyloge ... | 2003 | 12644681 |
| [molecular mapping of quantitative trait loci (qtl) for characters of vascular bundles in peduncle related to indica-japonica differentiation in rice (oryza sativa l.)]. | a doubled-haploid population, consisting of 81 dh lines derived from the f1 hybrid of a cross between an indica cultivar and a japonica cultivar, was used to map quantitative trait loci (qtl) controlling numbers of vascular bundles in peduncle, primary rachis branches and the ratio of vascular bundles to primary rachis branches (v/r ratio). for vascular bundles, three qtl were detected. among them, the qtl qvb-8 with the largest effect individually accounted for 31.1% of the total variation. two ... | 2002 | 12645263 |
| [in silico cloning of glucose-6-phosphate dehydrogenase cdna from rice (oryza sativa l.)]. | in silico cloning was a new strategy of gene cloning developed with the development of genome, est projects and bioinformatics. using wheat glucose-6-phosphate dehydrogenase cdna (clone: tagpdl) sequence as a querying probe, one highly homologous bac clone sequence was obtained from rice sequence database of genbank and the putative cdna sequence of rice glucose-6-phosphate dehydrogenase was assembled according to the wheat clone. furthermore, the full-length cdna of rice glucose-6-phosphate deh ... | 2002 | 12645266 |
| transgenic expression of trypsin inhibitor cme from barley in indica and japonica rice, confers resistance to the rice weevil sitophilus oryzae. | indica and japonica rice (oryza sativa l.) plants were transformed by particle bombardment with the itr1 gene encoding the barley trypsin inhibitor bti-cme, under the control of its own promoter that confers endosperm specificity, and the maize ubiquitin promoter. from 38 independent transgenic lines of indica (breeding line ir58) and 15 of the japonica (cv senia) selected, 22 and 11, respectively, expressed the barley inhibitor at detectable levels. the transgene was correctly translated as ind ... | 2003 | 12650522 |
| transcription factor binding study by capillary zone electrophoretic mobility shift assay. | regulation of gene expression through interaction of proteins with specific dna sequences is a central issue in functional genomics. capillary electrophoretic mobility shift assay is an efficient novel method for the investigation of sequence specific protein-dna interactions, allowing rapid and sensitive quantification of the complex formation. in this paper, we present a pilot study on capillary zone electrophoretic mobility shift assay (czemsa) to investigate the interaction between the trans ... | 2003 | 12652578 |
| effects of season-dependent irradiance levels and nitrogen-deficiency on photosynthesis and photoinhibition in field-grown rice (oryza sativa). | photoinhibition and acclimation of photosynthesis in rice plants grown under n-sufficient (ns) and n-deficient (nd) field conditions were investigated during the tropical wet (ws) and dry (ds) seasons in the philippines. diurnal patterns of co2 assimilation were examined. there was a transient peak in co2 assimilation in the leaves of the ns plants in the early morning during the ds and the ws, which was not seen in the nd plants in either season. nd leaves had lower ribulose bisphosphate carbox ... | 2003 | 12654034 |
| formation of solo-ltrs through unequal homologous recombination counterbalances amplifications of ltr retrotransposons in rice oryza sativa l. | we studied the dynamics of hopi, retrosat1, and rire3, three gypsy-like long terminal repeat (ltr) retrotransposons, in oryza sativa l. genome. for each family, we assessed the phenetic relationships of the copies and estimated the date of insertion of the complete copies through the evaluation of their ltr divergence. we show that within each family, distinct phenetic groups have inserted at significantly different times, within the past 5 myr and that two major amplification events may have oc ... | 2003 | 12654934 |
| tourist c transposable elements are closely associated with genes expressed in flowers of rice (oryza sativa). | tourist elements comprise a group of transposable elements in plants. one of these elements, tourist-osacata(a tourist c element), has been found in the 5; flanking region of a catalase gene, cata, in rice (oryza sativa). using reverse transcriptase-pcr (rt-pcr) analyses of leaves, roots, flowers and developing seeds of rice, we assessed the transcription levels of ten known genes containing tourist c elements, and of three additional putative genes for which expressed sequence tags (ests) inclu ... | 2003 | 12655403 |
| effect of high temperature on fine structure of amylopectin in rice endosperm by reducing the activity of the starch branching enzyme. | rice (oryza sativa l.) grain quality is affected by the environmental temperature it experiences. to investigate the physiological molecular mechanisms of the effect of high temperatures on rice grain, a non-waxy indica rice was grown under two temperature conditions, (29/35 degrees c) and (22/28 degrees c), during the ripening stage in two phytotrons. the activities and gene expression of key enzymes for the biosynthesis of amylose and amylopectin were examined. the activity and expression leve ... | 2003 | 12657298 |
| the redistribution of protein sulfur in transgenic rice expressing a gene for a foreign, sulfur-rich protein. | sulfur amino acid composition is an important determinant of seed protein quality. a chimeric gene encoding sunflower seed albumin (ssa), one of the most sulfur-rich seed storage proteins identified so far, was introduced into rice (oryza sativa) in order to modify cysteine and methionine content of the seed. analysis of a transgenic line expressing ssa at approximately 7% of total seed protein revealed that the mature grain showed little change in the total sulfur amino acid content compared to ... | 2003 | 12662304 |
| adsorption of radionuclides on silica and their uptake by rice plants from silica-multitracer solutions: the effects of ph. | we investigated the effect of solution ph on the adsorption on silica of a mixture radionuclides 83rb, 85sr, 54mn, 65zn, 88y, and 75se generated from irradiation of ag in a 135-mev/nucleon 12c beam accelerated by the riken ring cyclotron and 137cs obtained commercially. then, we related these findings to their uptake by a rice plant (oryza sativa l. cv. koshihikari) from a silica-multitracer solution at ph 4.3 +/- 0.2 and at 5.3 +/- 0.2. to evaluate both adsorption and uptake, precisely we used ... | 2002 | 12666841 |
| genome-wide intraspecific dna-sequence variations in rice. | genome-wide comparative analysis of the dna sequences of two major cultivated rice subspecies, oryza sativa l. ssp indica and oryza sativa l. ssp japonica, have revealed their extensive microcolinearity in gene order and content. however, deviations from colinearity are frequent owing to insertions or deletions. intraspecific sequence polymorphisms commonly occur in both coding and non-coding regions. these variations often affect gene structures and may contribute to intraspecific phenotypic ad ... | 2003 | 12667869 |
| a new aox homologous gene osim1 from rice (oryza sativa l.) with an alternative splicing mechanism under salt stress. | a differentially expressed osim1 gene was isolated from rice salt-tolerant mutant m-20 by differential display. sequence analysis revealed that the amino-acid sequence of osim1 showed 66% and 62% identity with ptox from tomato ( capsicum annuum) and atim from arabidopsis, both of which encoded chloroplast-orientated terminal oxidase. comparison of the nucleotide sequence of the osim1 cdna with its genomic sequence revealed that osim1 genomic dna contained nine exons and eight introns. a pseudo-t ... | 2003 | 12669200 |
| a proteomics approach to investigating promotive effects of brassinolide on lamina inclination and root growth in rice seedlings. | the application of brassinolide (bl) to the lamina joint region of rice (oryza sativa l., cv. nipponbare) seedlings caused marked bending of laminae, and bl influenced rice root growth under intact conditions. a remarkable increase in lamina inclination at bl 1 microm and an evident increase in root elongation at bl 0.01 microm were observed compared with the control. a total of 786 and 508 proteins extracted from the lamina joint and root, respectively, were detected in images of two-dimensiona ... | 2003 | 12673015 |
| highly efficient production and characterization of t-dna plants for rice ( oryza sativa l.) functional genomics. | we investigated the potential of an improved agrobacterium tumefaciens-mediated transformation procedure of japonica rice ( oryza sativa l.) for generating large numbers of t-dna plants that are required for functional analysis of this model genome. using a t-dna construct bearing the hygromycin resistance ( hpt), green fluorescent protein ( gfp) and beta-glucuronidase ( gusa) genes, each individually driven by a camv 35s promoter, we established a highly efficient seed-embryo callus transformat ... | 2003 | 12677401 |
| high resolution genetic mapping and candidate gene identification at the xa5 locus for bacterial blight resistance in rice ( oryza sativa l.). | the xa5 resistance gene from rice provides recessive, race-specific resistance to bacterial blight of rice caused by the pathogen xanthomonas oryzae pv oryzae. a high-resolution genetic map of the chromosomal region surrounding xa5 was developed by placing 44 dna markers on the distal end of rice chromosome 5. the basis for mapping was a pcr-based screening of 1,016 f(2) individuals derived from a cross between a near-isogenic line (nil) and its corresponding recurrent parent to identify recombi ... | 2003 | 12677405 |
| heat-tolerant basmati rice engineered by over-expression of hsp101. | rice is sensitive to high-temperature stress at almost all the stages of its growth and development. considering the crucial role of heat shock protein 101 (hsp 101) in imparting thermotolerance to cells, we introduced arabidopsis thaliana hsp101 (athsp101) cdna into the pusa basmati 1 cultivar of rice (oryza sativa l.) by agrobacterium-mediated transformation. stable integration and expression of the transgene into the rice genome was demonstrated by southern, northern and western blot analyses ... | 2003 | 12678556 |
| fine genetic mapping of a gene required for rice yellow mottle virus cell-to-cell movement. | the very high resistance to rice yellow mottle virus observed in the two rice varieties gigante ( oryza sativa) and tog 5681 ( o. glaberrima) is monogenic and recessive. bulked segregant analysis was carried out to identify aflp markers linked to the resistance gene. mapping of pcr-specific markers, caps and microsatellite markers on 429 individuals of an ir64 x gigante f(2) population pinpointed this resistance gene on the long arm of chromosome 4 in a 3.7-cm interval spanned by pcr markers. th ... | 2003 | 12679871 |