Publications

the timing of cortical granule fusion, content dispersal, and endocytosis during fertilization of the hamster egg: an electrophysiological and histochemical study.	to determine the temporal relationship between cortical granule exocytosis and the repetitive calcium transients, which are characteristic of mammalian fertilization, we monitored membrane addition from exocytosis during fertilization of hamster eggs. continuous measurement of membrane capacitance by applying a 3.1-na alternating current at 375 hz showed addition of cortical granule membrane. simultaneous measurement of membrane potential revealed each calcium transient by the appearance of tran ...	1994	8125193
purification and reconstitution of an intestinal na(+)-dependent neutral l-alpha-amino acid transporter.	using an improved reconstitution method, we have purified an na(+)-dependent neutral l-alpha-amino acid transporter from rabbit small intestine to apparent homogeneity. the preparation solubilized with octaethylene glycol dodecyl ether (c12e8) was purified by successive chromatographies on deae-toyopearl and lentil lectin-sepharose 4b columns. the transport activity was assayed by reconstitution of the protein into liposomes. the specific activity of the final preparation was 1364-fold that of b ...	1994	8132671
increased insulin receptor number and insulin responsiveness in a chicken hepatoma cell line.	insulin receptor number and insulin responsiveness were compared in a chicken hepatoma cell line (lmh) and in normal chicken hepatocyte (chep) cells cultured in the same conditions. lmh cells expressed two- to threefold more insulin receptors than chep cells, without significant changes in affinity. the tyrosine kinase activity of solubilized and lectin (lentil+wheat germ agglutinin; wga)-purified lmh receptors was higher than that of chep receptors. the atp hydrolytic activity previously observ ...	1994	8138746
glycosylation of human prolactin regulates hormone bioactivity and metabolic clearance.	to analyze the role of individual glycosylation pattern on prl biopotency, monomeric prolactin (prl), secreted by human prolactinoma cells in culture, was isolated by gel filtration and separated by affinity chromatography on concanavalin a-sepharose or lentil-agarose. these lectins allowed the isolation of prl glycoforms containing either biantennary, mannose-rich or fucosylated complex carbohydrate structures, respectively. endoglycosidase treatment and carbohydrate content of prl was found to ...	1993	8144855
interaction of lectins with human platelet serotonin transporter.	lectin affinity chromatography was used to demonstrate the glycoprotein nature of the serotonin (5-ht) transporter. the human platelet transporter protein was solubilized with 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (chaps), labelled with [3h]cyanoimipramine and chromatographed on lectin columns. wheat germ, gorse seed and lentil lectin-agarose columns specifically retained 5-ht transporter. peanut and horse gram lectins were ineffective. concanavalin a showed high non-specific ...	1994	8174615
purification to homogeneity of an active opioid receptor from rat brain by affinity chromatography.	active opioid binding proteins were solubilized from rat brain membranes in high yield with sodium deoxycholate in the presence of nacl. purification of opioid binding proteins was accomplished by opioid antagonist affinity chromatography. chromatography using the delta-opioid antagonist n,n-diallyl-tyr-d-leu-gly-tyr-leu attached to omega-aminododecyl-agarose (affi-g) (procedure a) yielded a partially purified protein that binds selectively the delta-opioid agonist [3h]tyr-d-ser-gly-phe-leu-thr ...	1994	8183950
true metabolisable energy values of some feedingstuffs.	1. the apparent metabolisable energy (ame) and the true metabolisable energy (tme) contents of some feedingstuffs were determined with cockerels. the test materials consisted of feed-grade lentil, lentil bran and wild vetch seed. 2. the average values of nitrogen lost, endogenous energy losses (eel) and endogenous energy losses corrected to zero-nitrogen balance (eeln) were found to be 1.18 g, 62.13 kj and 21.51 kj/bird/48h, respectively, from adult cockerels given an energy supply as 50 g gluco ...	1994	8199881
lectins as markers of rumen epithelial cell differentiation.	lectins of different carbohydrate specificities (gna (galanthus nivalis), con a (canavalia ensiformis), vfl (vicia faba), psl (pisum sativum), lca (lens culinaris), pna (arachis hypogaea; with or without prior neuraminidase treatment), wga (triticum vulgare), sba (glycine max), uea-i (ulex europaeus), lpa (limulus polyphemus), bs-i b4 (bandeiraea simplicifolia, isolectin b4)) were explored for use as differentiation markers of rumen epithelial cells in vivo and in vitro. lectins specific for man ...	1994	8206789
higher plant mitochondria encode an homologue of the nuclear-encoded 30-kda subunit of bovine mitochondrial complex i.	we describe the structure and expression of a wheat mitochondrial gene, which codes for a subunit of mitochondrial nadh dehydrogenase. the deduced protein sequence has 70% similarity to the 30-kda subunit of bovine mitochondrial complex i and 65% similarity to the 31-kda subunit of neurospora crassa complex i, components of the iron-sulfur-protein fraction, both nuclear-encoded proteins. we named this wheat mitochondrial gene as nad9. the wheat nad9 gene is transcribed in a single mrna of 0.9 kb ...	1993	8223639
demonstration of feline and canine platelet glycoproteins by immuno- and lectin histochemistry.	canine and feline platelet cytocentrifuge preparations (ccps), cryostat and paraffin-embedded bone marrow sections were used in this study. we evaluated whether platelets, megakaryocytes and megakaryocyte precursor cells could be labelled by monoclonal antibodies (y2/51, clb-thromb/1, hpl1) against human platelet membrane glycoprotein gp iiia and the gp iib/iiia complex or by the following 10 biotinylated lectins: concanavalin a (con a), lens culinaris agglutinin (lca), pisum sativum agglutinin ...	1993	8226111
schistosoma mansoni: surface membrane isolation with lectin-coated beads.	lectins from lens culinaris and arachis hypogaea immobilized on polyacrylamide beads were used for selective isolation of glycosylated surface membrane domains of adult schistosoma mansoni worms, and the method was compared with the membrane isolation procedure developed with polycationic (affi-gel) beads. the lentil lectin proved to be suitable for interaction with surface membrane components: an increment in the specific activities of tegumental phosphohydrolases was observed in the bound frac ...	1993	8231898
metacyclogenesis of leishmania (viannia) braziliensis in vitro: evidence that lentil lectin is a marker of complement resistance and enhanced infectivity.	axenic culture in modified grace's medium was used to induce metacyclogenesis of leishmania (viannia) braziliensis in vitro. morphological characteristics, lectin agglutination profiles, susceptibility to complement lysis, and infectivity in vivo were compared between metacyclic promastigotes and promastigotes in mid-log phase growth. short, arrow-like promastigotes and round, oval promastigotes were defined as putative metacyclic forms on the basis of being highly motile and free swimming, with ...	1993	8236409
increase of mannose residues, as salmonella typhimurium-adhering factor, on the cecal mucosa of germ-free chickens infected with eimeria tenella.	to study increase of the salmonella population in the cecum of chickens infected with eimeria tenella, quantitative changes in mannose residues on the cecal mucosa were investigated. inhibition of s typhimurium adherence to the cecum by a 2% carbohydrate (d-mannose, d-galactose, l-fucose, alpha-methyl-d-glucoside) in phosphate-buffered saline solution was examined. only d-mannose had inhibitory effects. whereas, d-galactose had somewhat enhancing effects on adherence of s typhimurium to the ceca ...	1993	8239135
purification and characterization of sperm-coating antigen, identified by a monoclonal antibody.	a procedure was designed for purification of a human seminal plasma-specific antigen (hsp-antigen) identified by a monoclonal antibody produced in this laboratory (mab 4e6). pooled human seminal plasma was fractionated by consecutively applied methods: affinity chromatography on lentil lectin sepharose, gel chromatography on ultrogel aca 34 and immunoaffinity on mab 4e6 coupled cnbr-sepharose 4b. the antigen-containing fraction obtained after this procedure was proved to be homogeneous when anal ...	1993	8250290
purification and characterisation of b2 bradykinin receptor from rat uterus.	a b2 bradykinin (bk) receptor was solubilised and partially purified from rat uterine membranes by a combination of ammonium sulphate precipitation, desalting on sephadex g-50, and hydroxyapatite and wheat germ agglutinin affinity chromatography. the partially purified bk receptor, enriched 1,500-fold, was then cross-linked to 125i-tyr0-bk using disuccinimidyl suberate and purified to homogeneity as a single protein species on two-dimensional gel electrophoresis with a molecular mass of 81 kda. ...	1994	8263517
comparative analysis of the immunoprotective abilities of glycosylated and deglycosylated parainfluenza virus type 3 surface glycoproteins.	the role of carbohydrate moieties on the immunoprotective ability of parainfluenza virus type 3 (piv-3) haemagglutinin-neuraminidase (hn) and fusion (f) glycoproteins was tested in hamsters. hn and f proteins were purified from detergent-solubilized virus by lentil-lectin affinity chromatography and deglycosylated by treatment with endoglycosidase f (endo f). immunization of hamsters with either 1 or 5 micrograms of mock-treated (glycosylated) affinity-purified proteins elicited strong haemagglu ...	1993	8277287
salivary gland surface carbohydrate variations in three species of the anopheles gambiae complex.	fluorescein isothiocyanate (fitc)-conjugated lectins (agglutinins) were employed as probes to distinguish between the various carbohydrates present on the surface of salivary glands of three species of mosquito of the anopheles gambiae complex. of twenty lectins tested, eight (concanavalin a- con a, lathyrus odoratus- loa, lens culinaris, pisum sativum-psa, vicia faba- vfa, triticum vulgaris, maclura pomifera- mpa and ulex europaeus) specifically reacted with the salivary gland membrane. both ma ...	1993	8279867
lectin receptors on iga isotypes.	it has been shown previously that secretory iga interacts with the mannose-specific lectin of escherichia coli. the purpose of the study described here was to evaluate whether the n-linked oligosaccharide chains of the human iga isotypes iga1 and iga2 differ in lectin receptor activity. a range of plant lectins specific for n-linked oligosaccharide chains were tested for their ability to precipitate iga1 and iga2 myeloma proteins, secretory iga and free secretory component. iga2 myeloma proteins ...	1994	8296163
edrf release from canine coronary artery by lectins.	1. the effect of wheat germ agglutinin (wga), concanavalin a (con a) and lentil lectin agglutinin (lca) were investigated on pre-contracted canine coronary artery rings in vitro. 2. in endothelium-intact canine coronary artery, contracted with the thromboxane a2-analogue, u46619, wga relaxed the tissue in a concentration-dependent manner, with an inhibitory concentration (ic50) of 112 +/- 17 nm (n = 6). in the absence of an endothelium, wga did not cause any relaxation of the tissue. 3. in endot ...	1993	8306089
lectin histochemistry of normal and neoplastic peripheral nerve sheath. 1. lectin binding pattern of normal peripheral nerve in man.	the binding patterns of lectins to normal peripheral nerves were examined. twelve biotinylated lectins were used in this study; canavalia ensiformis (con a), pisum sativum (psa), lens culinaris (lca), ricinus communis 1 (rca-1), arachis hypogaea (pna), glycine max (sba), sophora japonica (sja), bandeiraea simplicifolia 1 (bsl-1), triticum vulgaris (wga), succinylated wga (s-wga), ulex europaeus 1 (uea-1) and helix pomatia (hpa). cytoplasm of schwann cells and perineurial cells was stained by con ...	1993	8310810
g4 forms of acetylcholinesterase and butyrylcholinesterase in normal and dystrophic mouse muscle differ in their interaction with ricinus communis agglutinin.	differences in glycosylation between molecular forms of acetylcholinesterase (ache) and butyrylcholinesterase (buche) in muscle and serum of normal and dystrophic mice have been studied by means of their adsorption to immobilized lectins. application of a two-step extraction procedure, first with saline buffer, and second with saline buffer and triton x-100, brought into solution most of the muscle ache and buche activities. the ache activity was five times greater than that of buche in normal ( ...	1994	8312375
5-propyl-2-deoxyuridine induced interference with glycosylation in herpes simplex virus infected cells. nature of pdu-induced modifications of n-linked glycans.	in herpes simplex virus-infected (hsv) cells, the antiviral nucleoside analogue 5-n-propyl-2'-deoxyuridine (pdu) may, under certain circumstances, induce a pattern of interference with late steps in formation of n-linked glycans, resulting in increased availability of viral glycoproteins for neutralizing antibodies. the pdu-induced changes in n-linked glycans, released by pronase digestion of the hsv-specified glycoprotein gc-1, were investigated by using lectin affinity chromatography and bio-g ...	1993	8382038
structural analysis on the sugar chains of human alpha 1-antitrypsin: presence of fucosylated biantennary glycan in hepatocellular carcinoma.	chemical structures of the sugar chains of alpha 1-antitrypsin (aat) from patients with hepatocellular carcinoma (hcc) and from healthy individuals with a different affinity for lens culinaris agglutinin (lca) were examined by pyridylamination of their oligosaccharides and stepwise exoglycosidase digestion in combination with reversed-phase and size-fractionation high-performance liquid chromatography. we found that the lca-reactive species of aat from patients with hcc carried both the biantenn ...	1993	8390218
monoclonal antibody recognizes different quinone moieties in enzymes.	we produced monoclonal antibodies against the coenzyme pyrrolequinoline quinone (pqq). these antibodies were obtained by immunizing mice with pqq conjugated to a chemically modified polypeptide in order to induce a strong immune response. among the various antibodies obtained, one was found to bind (besides pqq and 6-hydroxydopamine conjugated to carrier proteins) several different quinoenzymes, namely lentil seedling and bovine serum diamine oxidases and methylamine dehydrogenase. this antibody ...	1993	8390461
glycan microheterogeneity of alpha 1-antitrypsin in serum and meconium from normal and cystic fibrosis patients by crossed immunoaffinoelectrophoresis with different lectins (con a, lca, wga).	in order to test whether abnormalities of glycosylation occur in cystic fibrosis (cf), the glycan microheterogeneity of alpha 1-antitrypsin (alpha 1-at) was studied in serum and meconium from normal individuals and patients with cystic fibrosis, by crossed immunoaffinoelectrophoresis (ciae) using free concanavalin a (con a), lens culinaris lectin (lca) and wheat germ agglutinin (wga). three main results emerged from this study: (1) modification of glycosylation in serum alpha 1-at from patients ...	1993	8403434
immunisation of macaques with siv env recombinants: specificity of t cell and antibody responses and evaluation of protective efficacy.	macaques were immunised with lentil lectin purified recombinant sivmac (bk28) derived gp160 (rgp160) with or without live vaccinia (vac)-env (bk28) priming, followed by a final boost with solid matrix antibody antigen (smaa)-gp160 (j5) complexes and challenged with the sivmac molecularly cloned virus j5m. rgp160 and vac-env plus gp160 induced strong ab responses against the homologous virus. live vac-env did not enhance or prolong the antibody response, however, t cell responses were stronger. a ...	1993	8411102
mesangial proliferative glomerulonephritis induced in rats by a lentil lectin and its antibodies.	experimental glomerulonephritis was induced in rats to investigate the consequence of the antigen-antibody interaction on the surface of glomerular endothelial cells (gens). a lectin, lens culinaris hemoagglutinin (lch), was first planted in the left kidney by isolated perfusion of a left kidney, and then the circulation was reestablished. rabbit anti-lch antibodies were injected from the tail vein 3 minutes after the recirculation of the left kidney, and acute glomerulonephritis ensued. fifteen ...	1993	8426083
growth and survival of shigella flexneri in common bangladeshi foods under various conditions of time and temperature.	survival and growth of shigella flexneri were assessed in various foods, including boiled rice, lentil soup, milk, cooked beef, cooked fish, mashed potato, mashed brinjal, and raw cucumber. growth at 25 and 37 degrees c and survival at 5 degrees c were observed by viable counts on macconkey agar. the organism grew well in all tested foods and growth increased from 10(5) to 10(8) to 10(10) cells per ml or g within 6 to 18 h after inoculation at 25 and 37 degrees c.	1993	8434933
thermodynamics of monosaccharide binding to concanavalin a, pea (pisum sativum) lectin, and lentil (lens culinaris) lectin.	titration calorimetry measurements of the binding of methyl alpha-d-mannopyranoside (me alpha man), d-mannopyranoside (man), methyl alpha-d-glucopyranoside (me alpha glu), and d-glucopyranoside (glu) to concanavalin a (con a), pea lectin, and lentil lectin were performed at 281 and 292 k in 0.01 m dimethylglutaric acid-naoh buffer (ph 6.9) containing 0.15 m nacl and mn+2 and ca+2 ions. the site binding enthalpies, delta h, are the same at both temperatures and range from -28.4 +/- 0.9 (me alpha ...	1993	8463297
purification and partial characterization of 200 kda oncofetal antigen from radiation induced murine lymphocytic lymphoma.	1. a 200 kda glycoprotein (gp200) oncofetal antigen was purified from solubilized membranes of a radiation-induced murine lymphocytic lymphoma cell line (xr11-5t), grown in syngeneic rfm mice, by successive gel chromatography of the active fraction on lentil lectin agarose, q- and s-sepharose and superose-12 using an fplc system. 2. a murine monoclonal antibody 115, produced by the syngeneic immunization of adult male c57bl/6n mice with 12-day mouse fetal cells, was used in a slot blot antibody ...	1993	8467950
dynamic light scattering studies of alpha iib beta 3 solution conformation.	the prototypical integrin receptor, alpha iib beta 3, isolated from the membrane fraction of human blood platelets by solubilization in triton x-100 (reduced) and affinity chromatography on lentil lectin-agarose, has been further purified by gel filtration chromatography in octyl glucoside to obtain the intact receptor complex in a form suitable for hydrodynamic measurements. the molecular weight [(6.0 +/- 0.2) x 10(3)] and stokes radius (2.3 +/- 0.1 nm) of detergent micelles formed in 0.03 m oc ...	1993	8471605
comparative effects of four legume species on plasma lipids and faecal steroid excretion in hypercholesterolaemic pigs.	the effect of four species of legume seeds on plasma cholesterol levels and faecal steroid excretion was studied in pigs. thirty-six growing boars were randomly allocated in groups of six to six diets which they ate continuously for 42 d. the diets fed were: 1, a semi-purified (sp; control group 1) diet; 2, sp + 10 g cholesterol/kg (control group 2); 3, 4, 5, 6, sp + cooked legumes (70:30, w/w; respectively baked beans (phaseolus vulgaris), peas (pisum sativum), lentils (lens culinaris medik.), ...	1993	8489997
isolation and characterization of the soluble and membrane-bound porcine cd44 molecules.	porcine cd44 was identified by the cross-reactive anti-human cd44 monoclonal antibody (mab) hermes-1. material which inhibited the hermes-1 mab binding to porcine lymphocytes was found in porcine intestinal efferent lymph and demonstrated to be a soluble form of porcine cd44. a protocol using non-affinity techniques was established to isolate this material from porcine lymph. the isolated soluble cd44 molecule consists of a single peptide with an apparent mw of about 50,000, containing the herme ...	1993	8495972
characterization of an eosinophilic leukemia cell differentiation factor (eldf) produced by a human t cell leukemia cell line, hil-3.	an adult t cell leukemia cell line, hil-3, constitutively secretes a factor which induces the phenotypical and functional eosinophilic differentiation of a human eosinophilic leukemia cell line, eol-1. biochemical characteristics of the factor, termed eosinophilic leukemia cell differentiation factor (eldf), were examined. eldf was precipitated by 35 to 65% saturated ammonium sulfate from the culture supernatants of hil-3 cells (hil-3 sup). eldf was eluted in a peak corresponding to a molecular ...	1993	8500576
immunoblot analysis of aspergillus fumigatus antigen with human antibodies and lectin probes.	components of culture filtrate antigen of aspergillus (a.) fumigatus were electrophoretically separated, transferred to hydrophobic durapore membrane, and then probed with patients' sera and lectins. using human sera from patients with aspergilloma and allergic bronchopulmonary aspergillosis (abpa), more than 13 igg binding components were identified. igg binding to high molecular weight components (hmw) of over 30 kda was observed in all patient sera tested, but igg binding to low molecular wei ...	1993	8507937
udp galactose:ceramide galactosyltransferase and glutamate/aspartate transporter. copurification, separation and characterization of the two glycoproteins.	the oligodendrocyte-specific udp-galactose:ceramide galactosyltransferase (cgt) is the key enzyme involved in the biosynthesis of the oligodendrocyte- and myelin-specific cerebrosides. the galactosyltransferase was isolated and purified to homogeneity from triton-x-100-solubilized rat brain microsomes by ion exchange, dye ligand and lectin affinity chromatography as a 64-kda protein homogenous in sds/page. it copurified with the brain-specific na(+)-dependent high-affinity l-glutamate/aspartate ...	1995	8521863
expression of human dopamine beta-hydroxylase in drosophila schneider 2 cells.	human dopamine beta-hydroxylase (dbh) has been expressed in transformed drosophila schneider 2 (s2) cells with yields of > 16 mg/l. most of the activity was found in the culture fluid. similarly, human neuroblastoma cells also secrete native dbh into the medium, but at a much lower level than recombinant drosophila cells. we have purified native and recombinant human dbh by a modified purification procedure using sp-sepharose, lentil lectin-sepharose and gel-filtration chromatography and carried ...	1996	8546710
lectin binding to extracellularly melanized microfilariae of brugia malayi from the hemocoel of anopheles quadrimaculatus.	binding patterns of fluorescein isothiocyanate (fitc)- and gold-conjugated lectins to extracellularly melanized sheathed and exsheathed microfilariae of subperiodic brugia malayi, isolated from and in situ in the abdominal hemocoel of anopheles quadrimaculatus 72-hr postinfection, were examined. five fitc-conjugated lectins [helix pomatia agglutinin (hpa), arachis hypogaea (peanut agglutinin-pna), triticum vulgaris (wheat germ agglutinin-wga), lens culinaris (lentil-lch), and concanavalin a (con ...	1995	8568282
growth related changes in sugar determinants on the surface of c6 glioma cells in culture: a cytochemical lectin-binding study.	the cell surface sugar determinants (cssd) were examined in c6 glioma cells in cultures at different conditions of growth by peroxidase conjugates of the lectins: peanut agglutinin (pna), ricinus communis agglutinin (rca), helix pomatia agglutinin (hpa), wheat germ agglutinin (wga), lentil agglutinin (lca), laburnum bork agglutinin (laba), and lotus agglutinin (tpa). it was found that the cells bound more intensively wga, lca, and rca compared to pna, hpa; the weakest staining was provided by la ...	1995	8568919
natural fermentation of lentils. influence of time, concentration and temperature on protein content, trypsin inhibitor activity and phenolic compound content.	lentil (lens culinaris var. vulgaris) flour was naturally fermented for 4 days at different temperatures (28 degrees c, 35 degrees c and 42 degrees c) and concentrations (79 milligrams, 150 milligrams and 221 milligrams). samples were analysed to establish the changes of total protein content and in vitro protein digestibility, trypsin inhibitor activity (tia) and phenolic compound content during natural fermentation of lentils. the preparation of lentil flour suspensions to be fermented caused ...	1995	8585337
rapid identification of lectin receptors and their possible function in sea urchin cell systems.	an assay using lectin derivatized agarose beads to rapidly and inexpensively identify cell surface lectin receptors was recently described by latham et al. (1995). in this earlier study, the assay was tested on large, early stage sea urchin embryo cells. in this study this assay was used to examine lectin receptors on small, later stage sea urchin embryo cells that are more typical of cells that most investigators deal with, to ascertain if cell size is a determining factor in the assay's validi ...	1995	8607287
effect of substituent on the thermodynamics of d-glucopyranoside binding to concanavalin a, pea (pisum sativum) lectin and lentil (lens culinaris) lectin.	titration calorimetry measurements of the binding of phenyl-alpha (alpha phoglu), 3-methoxy (3meoglu), fluorodeoxy and deoxy derivatives of alpha-d-glucopyranose (glu) to concanavalin a (cona), pea lectin and lentil lectin were performed at approx. 10 and 25 degrees c in 0.01 m dimethylglutaric acid/naoh buffer, ph 6.9, containing 0.15 m nacl and mn2+ and ca2+ ions. apparently the 3-deoxy, 4-deoxy and 6-deoxy as well as the 4-fluorodeoxy and 6-fluorodeoxy derivatives of glu do not bind to the le ...	1996	8645193
lectin histochemistry of pregnant rat uterine tissues.	glycoconjugate residues were examined in the rat uterus at days 10, 12 and 15 of pregnancy using 17 biotinylated lectins with specificities for a variety of carbohydrate moieties. a wide variety of glycoconjugate residues were detected in the cytoplasm of some antimesometrial and mesometrial decidual cells with lectins from canavalia ensiformis (con a), lens culinaris (lch) culinaris (lch), pisum sativum (psa), griffonia simplicifolia ii (gs-ii), triticum vulgaris (wga), griffonia simplicifolia ...	1996	8655407
chicken oviductal ecto-atp-diphosphohydrolase. purification and characterization.	an ecto-atp diphosphohydrolase (atpdase) was purified to homogeneity from vesiculosomes shed from chicken oviduct. first, the ecto-atpdase-enriched vesiculosomes were concentrated by filtration, differential centrifugation, and exclusion chromatography. next, the nonionic detergent, nonidet p-40, was used to extract the ecto-atpdase from vesiculosomal membranes, and the solubilized enzyme was further purified by ion exchange (deae-bio-gel) and lentil-lectin-sepharose 4b chromatography. in the fi ...	1996	8663133
comparative effects on blood lipids and faecal steroids of five legume species incorporated into a semi-purified, hypercholesterolaemic rat diet.	the cholesterolaemic effects in rats of a diet (vs) containing bambara groundnuts (vigna subterranea), a popular legume eaten in nigeria, were compared with diets pv, ps, lc and pl, containing baked beans (phaseolus vulgaris), marrowfat peas (pisum sativum), lentils (lens culinaris medik.) and butter beans (phaseolus lunatus) respectively. sixty sprague-dawley rats were fed on hypercholesterolaemic semi-purified diets supplemented with 10 g cholesterol and 5 g cholic acid/kg and formulated to pr ...	1996	8672408
binding properties of a blood group le(a+) active sialoglycoprotein, purified from human ovarian cyst, with applied lectins.	studies on the structures and binding properties of the glycoproteins, purified from human ovarian cyst fluids, will aid the understanding of the carbohydrate alterations occurring during the biosynthesis of blood group antigens and neoplasm formation. these glycoproteins can also serve as important biological materials to study blood group a, b, h, le(a), le(b), le(x), le(y), t and tn determinants, precursor type i and ii sequences and cold agglutinin i and i epitopes. in this study, the bindin ...	1996	8672551
a new subgroup of lectin-bound biliary proteins binds to cholesterol crystals, modifies crystal morphology, and inhibits cholesterol crystallization.	biliary proteins inhibiting or promoting cholesterol crystallization are assumed to play a major role in cholesterol gallstone pathogenesis. we now report a new group of biliary proteins that bind to cholesterol crystals, modify crystal morphology, and inhibit cholesterol crystallization. various glycoprotein mixtures were extracted from abnormal human gallbladder bile using lectin affinity chromatography on concanavalin a, lentil, and helix pomatia columns and were added to supersaturated model ...	1995	8675674
biochemical features of grain legume allergens in humans and animals.	peanuts and soybeans are the major legumes involved in human food allergy, although some data exist on adverse reactions to temperate legumes including pea, green bean, sweet lupin, and lentil. an increasing number of legume proteins or glycoproteins have been characterized as food allergens. limited data tend to indicate that they are usually different from legume inhalent allergens. cross-recognition among legume allergens is immunochemically frequent but clinically less common. a common featu ...	1996	8710238
microheterogeneity of serum transferrin in the diagnosis of hepatocellular carcinoma.	heterogeneous reactivity of human serum transferrin (tf) with lectins was analysed using patient sera to determine whether it can be used to distinguish patients with hepatocellular carcinoma (hcc) from those with liver cirrhosis (lc). microheterogeneity of tf was analysed by crossed immunoaffinity electrophoresis (ciae) with concanavalin a (con a) and lens culinaris agglutinin (lca). sample sera from 58 patients with hcc, 43 patients with lc and 10 normal controls were used in this study and th ...	1996	8713703
evolution and kinetics of monosaccharides, disaccharides and alpha-galactosides during germination of lentils.	the effect of light and seed rinsing during the germination of lentil seeds (lens culinaris var. vulgaris, cultivar magda-20) on the level of monosaccharides, disaccharides and alpha-galactosides (raffinose, ciceritol and stachyose) was investigated. the total soluble sugar content corresponded to about 9% of the mature seed weight, about 65% of which was alpha-galactosides. germination brought about a large decrease in alpha-galactosides: 18% to 40% losses after 3 days and 100% after 6 days. ho ...	1996	8717093
interaction of lectins with their ligand carbohydrate of alpha-fetoprotein: analysis by mixed-lectin affinity electrophoresis.	serum or ascites alpha-fetoprotein (afp) from patients with hepatocellular carcinoma and from a cord blood were analyzed by affinity electrophoresis with two lectins mixed in agarose gel in a combination of concanavalin a (con a) and lens culinaris agglutinin a (lca-a) or of erythroagglutinating phytohemagglutinin (e-pha) and allomyrina dichotoma lectin (allo a). con a- and lca-a-reactive afp-c2-l3 was not further retarded by mixing with either of the other lectin. it showed a mobility identical ...	1996	8740163
the identification of the phosphorylated 150/160-kda proteins of sarcoplasmic reticulum, their kinase and their association with the ryanodine receptor.	in the present work we studied the relationship between the phosphorylated 150- and 160-kda proteins and other sr proteins in the 150,000-170,000 range of molecular masses. on sds-page, the identification of their kinase, as well as the purification and structural interactions between these proteins and the rynodine receptor (ryr). the phosphorylated 150-kda protein was identified as sarcalumenin based on: (a) its cross-reactivity with three different monoclonal antibodies specific for sarcalume ...	1996	8765099
lectins do not distinguish between heterogenous ige molecules as defined by differential activity of an ige-dependent histamine releasing factor.	it has been suggested that differential histamine-releasing activity of an ige-dependent histamine-releasing factor (hrf), which has recently been cloned, is related to carbohydrate difference in the ige molecule. lectins are able to recognize specific glycoforms and might therefore be useful in characterizing the proposed heterogeneity of ige molecules. as one test of this hypothesis, we examined the histamine release potency of several well-characterized lectins on basophils passively sensitiz ...	1996	8765833
differences in the glycosylation of rat submandibular kallikreins.	the glycosylations of five different rat submandibular kallikreins, rk1, rk2, rk7, rk9 and rk10, vacuum-blotted onto nitrocellulose membranes, have been studied by means of labelled lectins using enhanced chemiluminescence detection. the results demonstrated the individual submandibular kallikreins are not heavily glycosylated in rats, but consistently show different patterns of glycosylation. following digestion of slot-blotted enzymes with peptide-n-glycosidase f (pngase): binding by lectin fr ...	1996	8785493
glycoconjugates of the human trabecular meshwork: a lectin histochemical study.	twelve specimens of resin-embedded human trabecular meshwork were probed with a panel of 21 biotinylated lectins, using an avidin-biotin peroxidase revealing system, in order to determine the normal pattern of saccharide expression in this tissue. high-mannose, intermediate and hybrid n-linked glycans, and complex n-linked bisected and non-bisected bi/tri-antennate glycans, as shown by the binding of canavalia ensiformis (cona), pisum sativum (psa), lens culinaris (lca) agglutinins and phaseolus ...	1995	8787965
lectin affinity electrophoresis of alpha-fetoprotein detected by immunoenzymatic and chemiluminescent amplification followed by direct scanning.	microdetermination of alpha-fetoprotein (afp) glycoforms by lectin affinity electrophoresis followed by chemiluminescence reaction using horseradish peroxidase (pod) or alkaline phosphatase (alp) in antibody-affinity blotting was developed. the intensity of chemiluminescence obtained by alp was greater than that by pod; however, the coefficient of variation with pod was less than that with alp. the optimized sensitivity of the chemiluminescence method with pod was two times that of the most sens ...	1996	8805850
prognostic significance of lens culinaris agglutinin a-reactive alpha-fetoprotein in small hepatocellular carcinomas.	lens culinaris agglutinin a-reactive fraction of alpha-fetoprotein (afp-l3) has been reported to be a useful marker in the early diagnosis of hepatocellular carcinoma (hcc). the aim of this study was to evaluate the prognostic value of afp-l3 for hcc.	1996	8831594
an anaphylaxis inhibitory factor present in mouse serum.	we have previously described that normal mouse serum inhibits the pca reaction mediated by ige. the present study attempts to characterize this pca inhibitory factor from the biologically active fraction of the serum. the physicochemical properties of this glycoprotein are the following: it is inactivated at 55 degrees c; it has a molecular weight between 182 kd and 240 kd, determined by gel filtration; it shows affinity to concanavalin a and lentil lectin but not to peanut agglutin; it demonstr ...	1996	8833169
effect of some spices as preservatives for storage of lentil (lens esculenta l.) seeds.	the high antimycotic activity of four kinds of spices, viz, chinese cassia, cinnamon, clove and thyme, were tested as preservatives for agricultural commodities. clove, thyme and a mixture of the four kinds of spice completely inhibited aflatoxin production on lentil seeds during eight weeks of incubation. chinese cassia and cinnamon partially inhibited the aflatoxin production.	1995	8846992
lectin binding to renaut bodies.	localization of sugar residues of renaut bodies in human sural nerves was studied using lectin histochemistry. lens culinaris specific to mannose and triticum vulgaris specific to glcnac bound to the capsular and core portions of renaut bodies strongly. arachis hypogaea specific to galb(1-3)galnac and ulex europaeus i specific to l-fucose bound to renaut bodies granularly. lectins specific to galactose or terminal galnac bound to nowhere. the perineurium showed similar lectin binding to renaut b ...	1995	8848942
the secretion of active recombinant human gastric lipase by saccharomyces cerevisiae.	the expression of the complete human gastric lipase (hgl) gene in saceharomyces cerevisiae grown in defined medium resulted in the secretion of active recombinant hgl (rec.hgl) to levels of up to approximately 11 mg/liter. of the total measurable hgl activity, 90% was detected by assaying intact cells, suggesting that the majority of rec.hgl produced was secreted but stayed attached to the cell wall. the remaining 10% was present in the growth medium and from this source active rec.hgl was purif ...	1996	8860647
human astrocytoma cells (u-87 mg) exhibit a specific substance p binding site with the characteristics of an nk-1 receptor.	to investigate substance p (sp) receptors on an established human astrocytoma cell line (u-87 mg), [3h][sar9,met(o2)11]-sp, a selective sp receptor agonist, was used to identify and characterize the cell membrane binding sites for sp. sp receptor mrna was examined by solution hybridization analysis, and the existence of sp binding protein on the surface of membranes was evaluated by flow cytometry using an anti-sp binding protein antibody. in u-87 mg and u-373 mg rna preparations, transcripts we ...	1996	8863485
effect of umami taste on pleasantness of low-salt soups during repeated testing.	in the present study the effects of the umami substances, monosodium glutamate (0.2%) and 5'-ribonucleotides (0.05%), on the acceptance of low-salt soups in two groups of subjects, one with low-salt (n = 21) and the other with high-salt (n = 23) preferences were assessed. the groups were presented with soups containing 0.3% sodium chloride (low-salt group) and 0.5% sodium chloride (high-salt group). the subjects three times consumed leek-potato or minestrone soup with umami and three times the o ...	1996	8873274
effect of natural fermentation on the content of inositol phosphates in lentils.	the effects of natural fermentation upon phytic acid and less phosphorylated inositol phosphates of lens culinaris var vulgaris cultivar magda-20 were investigated. seven fermentation runs were made following a 2(2) complete factorial design with three replicated centre points to study the effect of different conditions of temperature (28, 35 and 42 degrees c) and broth concentration (79, 150 and 221 g/l). samples were taken for each of them at daily intervals (0, 24, 48, 72 and 96 h). the ph va ...	1996	8873462
alpha-fetoprotein, carcinoembryonic antigen, and carbohydrate antigen 19-9-producing gallbladder cancer.	we report a rare case of alpha-fetoprotein (afp), carcinoembryonic antigen (cea), and carbohydrate antigen (ca) 19-9-producing gallbladder cancer with high levels of ca125 and protein induced by vitamin k absence or antagonist ii (pivka ii). a 63-year-old man was diagnosed with gallbladder cancer with metastases to the liver, based on ultrasonography and computed tomography of the abdomen showing multiple tumorous lesions in the liver and a thickened gallbladder wall. laboratory data showed high ...	1996	8887046
molecular and immunological characterization of soluble aggregated a/victoria/3/75 (h3n2) influenza haemagglutinin expressed in insect cells.	a/victoria/3/75 (h3n2)-derived cdna coding for a secreted haemagglutinin (ha0s) was cloned into the polyhedrin promoter-based pvl1392 transfer vector, and a recombinant baculovirus was isolated. 5 to 10 micrograms/ml of secreted ha were obtained following infection of spodoptera frugiperda-9 cells. gel filtration revealed the presence in the cell supernatant of immunoreactive ha molecules with varying m(r). the high m(r) fraction (aha0s) could be purified by matrex cellufine sulphate and lentil- ...	1996	8893793
glycoproteins in human parotid saliva assessed by lectin probes after resolution by sodium dodecyl sulphate-polyacrylamide gel electrophoresis.	human parotid salivary glycoproteins separated by gradient sodium dodecyl sulphate-polyacrylamide gel electrophoresis (sds-page) and electroblotted onto nitrocellulose have been investigated using a battery of biotinylated lectin probes of characterized sugar specificity. lectin binding, detected on blots using avidin-biotin complex (abc) and a chemiluminescence generating substrate, was recorded on photographic film and compared with the original fluorescein isothiocyanate (fitc) stained blots ...	1996	8907524
affinity electrophoretic determination of oligosaccharide specificity of butea monosperma agglutinin.	the oligosaccharide specificity of newly isolated butea monosperma agglutinin (bma) was determined by two-dimensional lectin affinity electrophoresis of alpha-fetoprotein (afp) with concanavalin a, lentil lectin, erythroagglutinating phytohemagglutinin and allomyrina dichotoma lectin, of which the specificities to known afp oligosaccharides had been established. effects of neuraminidase treatment on the reactivities of afp to the lectins were also studied. the results indicated that bma had the ...	1996	8907525
substrate specificity of lentil seedling amine oxidase.	copper diamine oxidase from lentil (lens culinaris) seedlings was shown to be able to catalyze the oxidative deamination of a wide range of aliphatic and aromatic monoamine compounds, including some amino acids. although the catalytic efficiencies were only 1-3% of that measured with the diamine substrate putrescine, they were still comparable to those of specialized monoamine oxidases. in particular, the lentil enzyme oxidized benzylamine and histamine with k(m) and vmax values similar to those ...	1996	8908374
post-fertilization changes in the zona pellucida glycoproteins of rat eggs.	the zona pellucida (zp) is the extracellular coat surrounding the mammalian egg. numerous evidence supports the role of zp carbohydrate residues as the specific sperm receptors. in this study we used lectins to study different distribution patterns of carbohydrate residues in the rat zp, and to follow changes at fertilization. zp were collected from follicular, ovulated, and fertilized eggs, incubated with one of 11 different biotin-labeled lectins, followed by avidin-fluorescein isothiocyanate ...	1996	8911967
purification and characterization of human lymphoblast n-acetylglucosamine-1-phosphodiester alpha-n-acetylglucosaminidase.	the enzyme n-acetylglucosamine-1-phosphodiester alpha-n-acetylglucosaminidase (ec 3.1.4.45; uncovering enzyme) catalyzed the removal of n-acetylglucosamine from the n-acetylglucosamine-alpha-phospho-mannose portion of selected lysosomal enzyme oligosaccharide chains, thereby forming the mannose 6-phosphate signal which is responsible for the targeting of these lysosomal enzymes for transport into lysosomes. the uncovering enzyme has been purified approximately 7000-fold to electrophoretic homoge ...	1996	8922957
evaluation of dowdo (wheat-milk gruel) in children with acute diarrhoea.	appropriate feeding practices have an important impact on diarrhoeal disease management in developing countries. we evaluated the efficacy of feeding dowdo, a wheat-milk gruel, traditionally used as a weaning food in the northern areas of pakistan. dowdo was compared with khitchri, a rice-lentil mixture, in acute diarrhoea through a randomized trial. seventy- six children between 6 and 36 months of age, with acute watery diarrhoea of less than seven days were recruited. after rehydration with st ...	1997	9056730
lactose intolerance in persistent diarrhoea during childhood: the role of a traditional rice-lentil (khitchri) and yogurt diet in nutritional management.	lactose intolerance is frequently encountered in children with persistent diarrhoea (pd). selection of an appropriate milk-based formulation is a major management problem in the developing world. in a consecutive series of studies, we evaluated the role of feeding a traditional rice-lentil (khitchri) diet alone (ky) or in combination with either soy formula (ky-soy) a dilute buffalo milk (ky-b), in children (age 6 months-3 years) with pd. serial observations of stool output, caloric intake and w ...	1997	9056732
sequential study of serum glycoprotein fucosylation in acute hepatitis.	alpha-fetoprotein is a useful diagnostic marker in hepatocellular carcinoma, during which its serum level increases and its glycan structure is hyperfucosylated. normally-expressed glycoproteins (alpha 1-antitrypsin and transferrin) are also hyperfucosylated in hepatocellular carcinoma. alpha-fetoprotein serum levels are also increased in conditions associated with hepatic regeneration, such as acute hepatitis. we conducted a longitudinal study of the alpha 1-6 fucosylation pattern of serum alph ...	1997	9059945
effects of concanavalin a on chondrocyte hypertrophy and matrix calcification.	resting chondrocytes do not usually undergo differentiation to the hypertrophic stage and calcification. however, incubating these cells with concanavalin a resulted in 10-100-fold increases in alkaline phosphatase activity, binding of 1,25(oh)2-vitamin d3, type x collagen synthesis, 45ca incorporation into insoluble material, and calcium content. on the other hand, other lectins tested (including wheat germ agglutinin, lentil lectin, pea lectin, phytohemagglutinin-l, and phytohemagglutinin-e) m ...	1997	9065448
a preliminary study on the effect of germination on saponin content and composition of lentils and chick peas.	two cultivars of chick peas (cicer arietinum) and one cultivar of lentils (lens culinaris) were subjected to germination in the dark for 6 days at 20 degrees c. soyasaponin vi, also known as soyasaponin beta g, a ddmp- (2,3-dihydro-2,5-dihydroxy-6-methyl-4h-pyran-4-one-) conjugated form of soyasaponin i, was the only saponin detected in both the unprocessed and germinated seed. no significant changes in saponin content were observed for chick peas or lentils after a 6-day germination.	1996	9123973
lectin-binding glycoconjugates in the subfornical organ of the rat.	in the rat subfornical organ (sfo), lectin-binding glycoconjugates were histochemically examined by 9 biotinylated lectins using a streptavidin-biotin peroxidase system. the strong or moderate binding of canavalia ensiformis (con a), lens culinaris (lca) and phaseolus vulgaris erythroagglutinin (epha) indicated overall distributions of high mannose, intermediate, hybrid n-linked, and non-bisected, bi/triantennate n-linked complex oligosaccharides in the rat sfo. nonsialylated terminal n-acetylgl ...	1997	9128064
interaction of proteases with legume seed inhibitors. molecular features.	after having found that raw black beans (phaseolus vulgaris) were toxic, while the cooked ones constitute the basic diet of the underdeveloped peoples of the world, in the sixties, our research directed by dr. jaffé, concentrated mainly around the detection and identification of the heat labile toxic factors in legume seeds. a micromethod for the detection of protease inhibitors (pi) in individual seeds was developed, for the purpose of establishing that the multiple trypsin inhibitors (ti) foun ...	1996	9137634
sensitivity and mass accuracy for proteins analyzed directly from polyacrylamide gels: implications for proteome mapping.	matrix-assisted laser desorption ionization (maldi) mass spectra have been obtained directly from thin-layer isoelectric focusing (ief) gels with as little as 700 femtomoles of alpha- and beta-chain bovine hemoglobin and bovine carbonic anhydrase, and 2 picomoles of bovine trypsinogen, soybean trypsin inhibitor, and bovine serum albumin all loaded onto a single lane. by soaking the gel in a matrix solution, matrix was deposited over the entire gel surface, allowing maldi scanning down complete l ...	1997	9150916
glycosylation of cholinesterase forms in brain from normal and dystrophic lama2dy mice.	differences in the oligosaccharides attached to acetyl- (ache) and butyrylcholinesterase (buche) forms in brain from control and merosin-deficient lama2dy dystrophic mice were investigated by means of their interaction with agarose-immobilized lectins. asymmetric ache, hydrophilic and amphiphilic ache and buche tetramers, and amphiphilic ache and buche monomers were identified in brain. all che forms were strongly adsorbed to the lectins concanavalin a (con a), lens culinaris (lca) and triticum ...	1997	9153638
further biochemical characterization of imidazoline binding sites from the human brainstem.	biochemical characteristics of imidazoline specific binding sites from the human brainstem were further investigated using [3h]idazoxan as radiolabeled ligand. the study of the interaction of [3h]idazoxan binding sites with heparin and lectins (soybean and lentil lectin) confirm the heterogeneity of these sites in the human brain. in fact, about 10-15% of [3h]idazoxan binding sites were retained by each of the three supports used, leading to the hypothesis that two populations of sites, with dif ...	1997	9182078
ozone stress modulates amine oxidase and lipoxygenase expression in lentil (lens culinaris) seedlings.	the effect of ozone stress on polyamine metabolism and membrane lipid peroxidation in lentil seedlings through the amine oxidase and lipoxygenase activity and expression has been investigated. ozone is shown to control the expression of these enzymes at the transcriptional level, down-regulating the amine oxidase gene and up-regulating the lipoxygenase gene. the decrease of amine oxidase activity correlated with the increase of putrescine concentration in the ozone-treated plantlets, whereas the ...	1997	9187375
functional properties of thermally treated legume flours.	functional properties of four thermally treated decorticated legume flours namely, bengal gram (cicer arietinum), black gram (phaseolus f1p4o roxb.), green gram (phaseolus aureus roxb.) and lentils (lens esculenta) were studied. samples with moisture levels of 3.2, 3.3, 1.3 and 5.0% for all four were subjected to dry heat treatment in a covered vessel in pressure cooker. (untreated flours served as controls. thermal treatment lowered nitrogen solubility profiles of all flours and increased water ...	1997	9205596
comparative evaluation of purified taenia solium glycoproteins and crude metacestode extracts by immunoblotting for the serodiagnosis of human t. solium cysticercosis.	a lentil-lectin purified glycoprotein (ll-gp) and a crude saline extract of taenia solium metacestodes were compared for the immunodiagnosis of human cysticercosis by immunoblotting. the ll-gp preparation was 95% sensitive for antibodies against a range of seven antigens with molecular masses of 50 to 13 kda, whereas the sensitivity of the crude saline extract for the detection of antibodies against two major polypeptide molecules (26 and 8 kda) was 91%. specificity was 100% with both sets of di ...	1997	9302208
glycosylation variants of human tsh selectively activate signal transduction pathways.	the oligosaccharide chains of pituitary glycoprotein hormones such as human thyroid-stimulating hormone (htsh) have been shown to be important in biosynthesis, subunit association, secretion and bioactivity. however, the exact biological significance of these glycosylation variants (isoforms) remains controversial. the aim of this paper is to investigate the role of htsh glycosylation variants in signal transduction. human pituitary standard tsh (2nd international reference preparation 80/558; i ...	1997	9324060
an igg monoclonal antibody against dictyostelium discoideum glycoproteins specifically recognizes fucalpha1,6glcnacbeta in the core of n-linked glycans. localized expression of core-fucosylated glycoconjugates in human tissues.	core fucosylation of n-linked oligosaccharides (glcnacbeta1, 4(fucalpha1,6)glcnacbeta1-asn) is a common modification in animal glycans, but little is known about the distribution of core-fucosylated glycoproteins in mammalian tissues. two monoclonal antibodies, cab2 and cab4, previously raised against carbohydrate epitopes of dictyostelium discoideum glycoproteins (crandall, i. e. and newell, p. c. (1989) development 107, 87-94), specifically recognize fucose residues in alpha1,6-linkage to the ...	1997	9325301
afp isoforms and their clinical significance (overview).	afp has maintained great clinical relevance as target tumor marker of germ cell tumors (yst = yolk sac tumors) and hepatocellular cancer (hcc) besides its occasional elevation in other malignancies. its organ and tumor specificity is further limited by elevation in benign liver diseases (bld) and in pregnancy. the binding of various lectins to its carbohydrate chain (5%) in addition to mere isoelectric focussing has been successfully used in the differentiation of afp source and disease. the pri ...	2005	9329566
autopsy analysis of clinically unilateral exfoliation syndrome.	to study the pathogenesis of clinically unilateral exfoliation syndrome by localizing exfoliation deposits in involved and fellow eyes during autopsy.	1997	9331264
development of the competence of bovine oocytes to release cortical granules and block polyspermy after meiotic maturation.	bovine immature oocytes do not have the ability to block polyspermic penetration. the present study was conducted to determine whether this is correlated to cortical granule (cg) distribution and the competence of oocytes to release cg upon sperm penetration, and whether the ability of bovine oocytes to release cg develops during in vitro maturation. fluorescein isothiocyanate-conjugated lens culinaris agglutinin was used for detecting cg in immature and mature oocytes before and after sperm pen ...	1997	9338596
conformational analysis of biantennary glycans and molecular modeling of their complexes with lentil lectin.	some mannose-binding legume lectins show higher affinity for fucosylated glycans than for glycans without fucose. these lectins possess a secondary binding site. owing to the possibility of additional fucose binding, oligosaccharides adopt different conformations depending on whether they contain fucose or not. to study these conformational differences, complexes of fucosylated and unfucosylated glycans with lens culinaris lectin have been modeled. starting points were x-ray structures of lentil ...	1997	9346821
characterization of new simian foamy viruses from african nonhuman primates.	simian foamy viruses (sfv) are exogenous retroviruses present in most if not all nonhuman primate species. baboons and other african monkey species are known to harbor sfvs, yet there is presently no data in regard to their genetic relationship. here we studied sfvs from baboons as compared to other sfvs isolated from a hamlyn's guenon, a patas monkey, and a vervet. by western blot analysis, the gag precursor proteins (p74/p70) were detected from all sfvs. in addition, the envelope glycoproteins ...	1997	9356346
induction of eosinophil chemotactic factor production from human peripheral blood mononuclear cells by solubilized ball-1, a b cell lymphoma line.	we have previously shown that irradiated ball-1, a b lymphoma line, stimulates okt4 t cells to produce a unique eosinophil chemotactic factor (ecf) which suppresses the respiratory burst of eosinophils. in the present experiments, we found that solubilized ball-1 also stimulated peripheral blood mononuclear cells (pbmc) to produce ecf activity in a dose-dependent manner. pbmc treated with solubilized ball-1 produced ecf within 24 h. ecf production-inducing factor (ecf-if) had affinity with lenti ...	1997	9363915
establishment of the block against sperm penetration in parthenogenetically activated bovine oocytes matured in vitro.	the ability of a single electric pulse to mimic a block against sperm penetration in bovine oocytes matured in vitro was investigated. confocal laser scanning microscopy detected a global loss of spots, presumed to be cortical granules, stained with lens culinaris agglutinin, in pulsed oocytes. transmission electron microscopy revealed that cortical granule exocytosis occurred within 1 min of stimulation and the number of remaining cortical granules was significantly reduced in all pulsed oocyte ...	1997	9370979
the effects of carcinogenic methylcholanthrene on carbohydrate residues of nk cells.	the present study examines the effect of methylcholanthrene (mca), a a carcinogenic polycyclic hydrocarbon, on the carbohydrate receptor determinants (rd) on natural killer (nk) cell surface using the bead-coupled lectin assay. murine nk cells exhibited different degrees of preferential binding to the specific lectins tested. of the ten lectins tested, five exhibited a positive binding affinity while the remaining five exhibited no or insignificant binding. nk cells bind to beads derivatized wit ...	2017	9399418
inhibitory activity of a naturally occurring heterocyclic beta-substituted alanine, beta-(isoxazolin-5-on-4-yl)-l-alanine, on the l-glutamate/l-aspartate transporter (glast) expressed in xenopus oocytes.	excitatory amino acid (eaa) transporters are of physiological importance in the regulation of the extracellular concentration of excitatory amino acids and the neuroexcitation in cns. among four identified transporters, the na+-dependent high-affinity l-glutamate/l-aspartate transporter (glast) is highly expressed in glial cells. here, we report a naturally occurring inhibitor of glast, derived from bovine retina, using the xenopus oocyte expression system. beta-(isoxazolin-5-on-4-yl)-l-alanine ...	1997	9450691
molecular cloning and localization of human syntaxin 16, a member of the syntaxin family of snare proteins.	we have cloned a new member of the syntaxin family of proteins, designated human syntaxin 16 (hsyn16). the open reading frame encodes a polypeptide of 307 amino acids with potential coiled-coil domains and a carboxy-terminal hydrophobic tail, which is characteristic of other members of the syntaxin family. the encoded polypeptide bears sequence homology to known syntaxin molecules. northern blot analysis revealed a single transcript that is fairly ubiquitous, being slightly more enriched in hear ...	1998	9464276
segregation of the pathways leading to cortical reaction and cell cycle activation in the rat egg.	at fertilization of the mammalian egg, resumption of the cell cycle and the cortical reaction are two events of egg activation, correlated with an increase in intracellular ca2+ concentration and activation of protein kinase c. to evaluate the pathways leading to both events, rat eggs were parthenogenetically activated by the calcium ionophore ionomycin, or by the protein kinase c activators 12-o-tetradecanoyl phorbol-13-acetate (tpa) or 1-oleoyl-2-acetylglycerol (oag). cortical granule exudate ...	1998	9472928
alpha-fetoprotein-producing rectal carcinoma--a case report.	a case with an alpha-fetoprotein (afp)-producing carcinoma originating from the rectum is described. a 71-year-old male patient, who underwent a rectectomy for rectal carcinoma, developed space occupying lesions in the liver and a remarkable afp elevation (220,000 ng/ml) in the 6th month postoperatively, and then expired one year later. histologically, the rectal carcinoma consisted of well-differentiated adenocarcinomas, and contiguous cancerous cells proliferating in either a medullary or trab ...	1997	9476478
detection of glycated fetal human lens crystallins by concanavalin-a and aldehyde staining.	the binding of the lectin concanavalin-a (con-a) to crystallins was investigated. for this purpose, human fetal and juvenile lens crystallins were isofocused and specifically stained brown for glycoproteins by the lectin con-a, and purple by the periodic acid schiff's reagent (pas). in reference experiments protein bands were stained with coomassie blue for proteins. since con-a is a protein with glucose-specific receptors, the following crystallins, glycated with this sugar, were visualized aft ...	1997	9483490
na-p(i) cotransport sites in proximal tubule and collecting tubule of winter flounder (pleuronectes americanus).	localization of a recently described and cloned na-pi cotransport system from flounder was investigated by reverse transcription-polymerase chain reaction (rt-pcr) of microdissected tubules and by immunocytochemistry of kidney of winter flounder. histological examination showed a small glomerulus, an extremely short proximal tubule pi with a selective affinity to lens culinaris agglutinin from lentils, and an extensive second proximal tubule segment pii (> 90% of proximal tubules), consisting of ...	1998	9486233