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post-infection development and histopathology of meloidogyne incognita in resistant cotton.the numbers of meloidogyne incognita larvae which migrated from cotton roots declined over a 16-day period, but the difference in numbers migrating from resistant and susceptible cultivars was not significant. larvae penetrated susceptible roots, matured, and reproduced within 14 days following inoculation, whereas nematode development in the resistant roots was greatly retarded. three types of histological responses were observed in infected, resistant roots, and these correlated with the degre ...197419319359
population dynamics of plant nematodes in cultivated soil: effect of summer cover crops in newly cleared land.five nematode species were studied for ability to develop on seven summer cover crops in rotation with tomato transplants grown every third year. increase of tylenchorhynchus claytoni, trichodorus christiei, pratylenchus brachyurus, helicotylenchus dihystera, and xiphinema americanum in newly cleared soil varied with different cover crops. no substantial nematode population increases occurred until the third summer of crop growth. all species except x. americanum and h. dihystera developed best ...197019322300
hatching response of meloidogyne incognita acrita to electric shock.the influence of electric shock on hatch of meloidogyne incognita acrita from egg masses taken from roots of 'acala sj-i' cotton (gossypium hirsutum l.) was studied. egg masses in tap water were individually placed between the tips of needle electrodes 1 mm apart and exposed to potentials of l, 10, 20, and 60 vdc/mm at 1, 1, 1, and 86 milliamperes dc, respectively, for periods of 2 and 60 seconds. hatched larvae were counted at five-day intervals for 60 days. of the eight treatment combinations ...197019322315
role of nematodes and soil-borne fungi in cotton stunt.the nematodes, pratylenchus brachyurus, trichodorus christiei, and t. porosus and the soil-borne fungi, rhizoctonia solani, pythium debaryanum, p. irregulare, p. ultimum, and fusarium spp. were the pathogens most frequently found in the roots and rhizosphere of field-grown cotton (gossypium hirsutum) showing "stunt" symptoms. field-plot application of the nematicide d-d (l,2-dichloropropane, 1,3-dichloropropene) at 373.4 liter/ha (40 gal/a) significantly increased plant growth and yield. a fungi ...197119322335
interaction of rotylenchulus reniformis, soil salinity, and cotton.rotylenchulus reni]ormis occurred equally in relatively non-saline (4.0 mmhos/cm) and highly-saline (16.5 mmhos/cm) soils in sampling transects across zones of depressed plant growth in six texas cotton fields.results from greenhouse pot experiments indicated progressive positive interaction of salinity and r. reni[ormis pathogenicity in the range 6-18 mmhos/cm.197119322365
reaction of field-grown sericea lespedeza to selected meloidogyne spp.five sericea lespedeza (lespedeza cuneata [dumont] g. don) breeding lines resistant to meloidogyne incognita, m. incognita acrita, and m. hapla as seedlings in greenhouse tests and two varieties were resistant to m. incognita acrita in field experiments. root-knot galling and larvae numbers were less for resistant entries than for the susceptible check when grown in root-knot infested field soil for three growing seasons. forage yields were as much as 57 times greater for resistant entries than ...197119322393
influence of incubation solution on the rate of recovery of pratylenchus brachyurus from cotton roots.the rate of recovery of pratylenchus brachyurus from cotton roots was enhanced when the tissue was incubated in solutions containing 10 ppm ethoxyethyl mercuric chloride, 50 ppm dihydrostreptomycin sulfate, 50, 100, or 1,000 ppm diisobutylphenoxethyl dimethyl benzyl ammonium chloride, or mixtures of these compounds. incubation in 10 or 100 ppm zinc sulfate, zinc chloride, or magnesium chloride also enhanced the rate of recovery. incubation solutions containing 1 or 1,000 ppm zinc chloride or mag ...197119322395
interaction of novel dobzhansky-muller type genes for the induction of hybrid lethality between gossypium hirsutum and g. barbadense cv. coastland r4-4.hybrid lethality was identified in interspecific hybrids between two cotton species, gossypium hirsutum and g. barbadense cv. coastland r4-4 (r4-4). genetic analysis indicated that the lethal symptom was controlled by two dominant complementary genes, one from g. hirsutum and another from r4-4. microsatellite mapping identified the location of the causal gene in g. hirsutum as chromosome d8, while the r4-4 gene was placed on chromosome d11. our data indicate that these genes conform to the dobzh ...200919330312
genetic diversity evaluation of some elite cotton varieties by rapd analysis.random amplified polymorphic dna (rapd) analysis was used to evaluate the genetic diversity of elite commercial cotton varieties. twenty two varieties belonging to gossypium hirsutum l. and one to g. arboreum l. were analyzed with 50 random decamer primers using the polymerase chain reaction (pcr). forty nine primers detected polymorphism in all 23 cotton varieties, while one produced monomorphic amplification profiles. a total of 349 bands were amplified, 89.1% of which were polymorphic. cluste ...199719352756
a specialized outer layer of the primary cell wall joins elongating cotton fibers into tissue-like bundles.cotton (gossypium hirsutum) provides the world's dominant renewable textile fiber, and cotton fiber is valued as a research model because of its extensive elongation and secondary wall thickening. previously, it was assumed that fibers elongated as individual cells. in contrast, observation by cryo-field emission-scanning electron microscopy of cotton fibers developing in situ within the boll demonstrated that fibers elongate within tissue-like bundles. these bundles were entrained by twisting f ...200919369592
a new interspecific, gossypium hirsutum x g. barbadense, ril population: towards a unified consensus linkage map of tetraploid cotton.we report the development of a new interspecific cotton recombinant inbred line (ril) population of 140 lines deriving from an interspecific cross between gossypium hirsutum (gh) and g. barbadense (gb), using the same two parents that have served for the construction of a bc(1) map and for the marker-assisted backcross selection program underway at cirad. two marker systems, microsatellites and aflps, were used. an important feature of the ril population was its marked segregation distortion wit ...200919387609
ghzfp1, a novel ccch-type zinc finger protein from cotton, enhances salt stress tolerance and fungal disease resistance in transgenic tobacco by interacting with gzird21a and gzipr5.* zinc finger proteins are a superfamily involved in many aspects of plant growth and development. however, ccch-type zinc finger proteins involved in plant stress tolerance are poorly understood. * a cdna clone designated gossypium hirsutum zinc finger protein 1 (ghzfp1), which encodes a novel ccch-type zinc finger protein, was isolated from a salt-induced cotton (g. hirsutum) cdna library using differential hybridization screening and further studied in transgenic tobacco nicotiana tabacum cv. ...200919402879
alternate row placement is ineffective for cultural control of meloidogyne incognita in cotton.the objective of this study was to determine if planting cotton into the space between the previous year's rows reduces crop loss due to meloidogyne incognita compared to planting in the same row every year. row placement had a significant (p </= 0.05) effect on nematode population levels only on 8 july 2005. plots receiving 1,3-dichloropropene plus aldicarb had lower nematode population levels than non-fumigated plots on 24 may and 8 july in 2005, but not in 2004. the effect of nematicide treat ...200819440259
effect of fumigation on rotylenchulus reniformis population density through subsurface drip irrigation located every other furrow.plots naturally infested with rotylenchulus reniformis were sampled in the spring of 2006 and 2007 at depths of 15 and 30 cm in the bed, furrow over the drip tape, and "dry" furrow, and at approximately 40 to 45 cm depth in the bed and dry furrow. then, 1,3-dichloropropene (telone ec) was injected into the subsurface drip irrigation at 46 kg a.i./ha, and 3 to 4 weeks later the plots were resampled and assayed for nematodes. the transformed values for nematode population density (ivlrr) before fu ...200819440261
frequency of alleles conferring resistance to the bacillus thuringiensis toxins cry1ac and cry2ab in australian populations of helicoverpa punctigera (lepidoptera: noctuidae) from 2002 to 2006.helicoverpa punctigera and helicoverpa armigera (hübner) (lepidoptera: noctuidae) are important pests of field and horticultural crops in australia. the former is endemic to the continent, whereas the latter is also distributed in africa and asia. although h. armigera rapidly developed resistance to virtually every group of insecticide used against it, there is only one report of resistance to an insecticide in h. punctigera. in 1996 the australian cotton industry adopted ingard, which expresses ...200919449655
molecular diagnostic for boll weevil (coleoptera: curculionidae) based on amplification of three species-specific microsatellites.the boll weevil, anthonomus grandis grandis boheman (coleoptera: curculionidae), is a serious pest of cultivated cotton, gossypium hirsutum l., in the americas, and reinfestation of zones from which they have been eradicated is of perpetual concern. extensive arrays of pheromone traps monitor for reintroductions, but occasionally the traps collect nontarget weevils that can be misidentified by scouts. for example, the congeneric pepper weevil, anthonomus eugenii cano, and other superficially sim ...200919449658
a synthetic auxin (naa) suppresses secondary wall cellulose synthesis and enhances elongation in cultured cotton fiber.use of a synthetic auxin (naphthalene-1-acetic acid, naa) to start (gossypium hirsutum) ovule/fiber cultures hindered fiber secondary wall cellulose synthesis compared with natural auxin (indole-3-acetic acid, iaa). in contrast, naa promoted fiber elongation and ovule weight gain, which resulted in larger ovule/fiber units. to reach these conclusions, fiber and ovule growth parameters were measured and cell wall characteristics were examined microscopically. the differences in fiber from naa and ...200919479259
expression and localization of ghh6l, a putative classical arabinogalactan protein in cotton (gossypium hirsutum).arabinogalactan proteins (agps) are a large family of highly glycosylated of hydroxyproline-rich glycoproteins that play important roles in plant growth, development, and signal transduction. a cdna encoding a putative classical agp named ghh6l was isolated from cotton fiber cdna libraries, and the deduced protein contains 17 copies of repetitive motif of x-y-proline-proline-proline (where x is serine or alanine and y is threonine or serine). northern blotting analysis and quantitative rt-pcr re ...200919499153
identification and molecular mapping of a fusarium wilt resistant gene in upland cotton.fusarium wilt (fw) is one of the most economically damaging cotton diseases worldwide, causing yellowing, wilting, defoliation, vascular tissue damage and ultimately death. identification of molecular markers linked to fw genes is vital to incorporate resistance into elite cotton cultivars. an intraspecific f(2) in gossypium hirsutum l. was developed by crossing with a highly resistant cultivar zhongmiansuo 35 (zms35) and a susceptible cultivar junmian 1 to screen simple sequence repeats (ssrs) ...200919506830
transcript profiling during fiber development identifies pathways in secondary metabolism and cell wall structure that may contribute to cotton fiber quality.a global gene expression profiling study at different stages of fiber development was undertaken on two cotton species cultivated for fiber, gossypium hirsutum (l.) and g. barbadense (l.). a large proportion of the genome was expressed during both fiber elongation and subsequent secondary cell wall thickening. there was a major shift in abundance of transcripts for gene regulation, cell organization and metabolism between fiber elongation and fiber thickening that was fundamentally similar in bo ...200919520671
molecular and biochemical evidence for phenylpropanoid synthesis and presence of wall-linked phenolics in cotton fibers.the mature cotton (gossypium hirsutum l.) fiber is a single cell with a typically thickened secondary cell wall. the aim of this research was to use molecular, spectroscopic and chemical techniques to investigate the possible occurrence of previously overlooked accumulation of phenolics during secondary cell wall formation in cotton fibers. relative quantitative reverse transcription-polymerase chain reaction analysis showed that ghcad6 and ghcad1 were predominantly expressed among seven gene ho ...200919566641
phenotypic and molecular evaluation of cotton hairy roots as a model system for studying nematode resistance.agrobacterium rhizogenes-induced cotton (gossypium hirsutum l.) hairy roots were evaluated as a model system for studying molecular cotton-nematode interactions. hairy root cultures were developed from the root-knot nematode (rkn) (meloidogyne incognita [kofoid and white] chitwood, race 3)-resistant breeding line m315 and from the reniform nematode (rn) (rotylenchulus reniformis linford & oliveira)-resistant accession gb713 (g. barbadense l.) and compared to a nematode-susceptible culture derive ...200919578854
molecular cloning and characterization of five genes encoding pentatricopeptide repeat proteins from upland cotton (gossypium hirsutum l.).the pentatricopeptide repeat (ppr) protein family is one of the largest and most complex families in plants. these proteins contain multiple 35-amino acid repeats that are proposed to form a super helix capable of binding rna. ppr proteins have been implicated in many crucial functions broadly involving organelle biogenesis and plant development. in this study, we identified many genes encoding ppr protein in upland cotton through an extensive survey of the database of gossypium hirsutum. furthe ...201019578975
molecular characterization and pathogenicity of tomato yellow leaf curl virus in china.several tomato production regions in china were surveyed for tomato yellow leaf curl disease (tylcd), and 31 tomato leaf samples showing tylcd-like symptoms were collected. the partial or full-length genomes of these isolates were sequenced and tomato yellow leaf curl virus (tylcv) was detected in shanghai, zhejiang, jiangsu shandong and hebei provinces of china. the tylcv isolates found in china share high sequence identity ([98%) and have more than 97% sequence identity with tylcvil[ il:reo] ( ...200919590945
flower-inhabiting frankliniella thrips (thysanoptera: thripidae), pesticides, and fusarium hardlock in cotton.cotton hardlock caused by fusarium verticillioides (sacc. nirenberg) can reduce cotton, gossypium hirsutum l., yields > 70% in the southeastern united states. the spores infect flowers on the day of pollination, resulting in hardlock, which is the failure of the fiber to fluff as the boll opens at maturity. frankliniella spp. thrips (thysanoptera: thripidae) inhabiting the flowers are hypothesized to increase hardlock by spreading the conidia or by creating entranceways for the germinating fusar ...200919610399
trichogramma (hymenoptera: trichogrammatidae) ecology in a tropical bt transgenic cotton cropping system: sampling to improve seasonal pest impact estimates in the ord river irrigation area, australia.trichogramma westwood (hymenoptera: trichogrammatidae) cause high mortality rates in the potentially resistant pest species, helicoverpa armigera (hübner) (lepidoptera: noctuidae), and are considered integral to the resistance management plan for bacillus thuringiensis transgenic cotton, gossypium hirsutum l., production in the ord river irrigation area (oria), western australia. measured as percentage of parasitism, trichogramma activity seems highly variable over time; yet, it contributes sign ...200919610415
cry2ab tolerance response of helicoverpa armigera (lepidoptera: noctuidae) populations from crylac cotton planting region.helicoverpa armigera (hübner) (lepidoptera: noctuidae) is an important lepidopteran pest of cotton, gossypium hirsutum l., in asia. transgenic cotton expressing the cry1ac gene from bacillus thuringiensis (bt) has been widely planted for control of this pest. for managing the potential risk from resistance evolution in this pest, a new transgenic bt cotton containing cry1ac and cry2ab genes (gene pyramided strategy) was designed as a successor of cry1ac cotton. this article reports on levels of ...200919610441
effects of pink bollworm resistance to bacillus thuringiensis on phenoloxidase activity and susceptibility to entomopathogenic nematodes.widespread planting of crops genetically engineered to produce insecticidal toxins from the bacterium bacillus thuringiensis (bt) imposes selection on many key agricultural pests to evolve resistance to bt. fitness costs can slow the evolution of bt resistance. we examined effects of entomopathogenic nematodes on fitness costs of bt resistance in the pink bollworm, pectinophora gossypiella (saunders) (lepidoptera: gelechiidae), a major pest of cotton, gossypium hirsutum l., in the southwestern u ...200919610442
quantification of cry1ac and cry1f bacillus thuringiensis insecticidal proteins in selected transgenic cotton plant tissue types.quantitative enzyme-linked immunosorbent assays were used to characterize the geographical (locations) and temporal (through 6 wk) expression of crylac, from bacillus thuringiensis variety kurstaki, and cry1f, from b. thuringiensis variety aizawai, in transgenic cotton, gossypium hirsutum l., plant structures. terminal leaves, squares (flower buds), flowers, bolls (fruit), and mature leaves located five and eight nodes below the terminal apex were sampled during weeks 2, 4, and 6 after the initi ...200919610451
sucrose synthase affects carbon partitioning to increase cellulose production and altered cell wall ultrastructure.overexpression of the gossypium hirsutum sucrose synthase (susy) gene under the control of 2 promoters was examined in hybrid poplar (populus alba x grandidentata). analysis of rna transcript abundance, enzyme activity, cell wall composition, and soluble carbohydrates revealed significant changes in the transgenic lines. all lines showed significantly increased susy enzyme activity in developing xylem. this activity manifested in altered secondary cell wall cellulose content per dry weight in al ...200919625620
[effects of mepiquat chloride on inorganic elements contents in seeds of transgenic insect-resistant cotton determined by icp-ms].to evaluate the effects of a worldwide used plant growth regulator mepiquat chloride on the nutrition value and safety of seeds of transgenic bt cotton, inorganic element components and contents in seeds of bt cotton (gossypium hirsutum cv. guoxin 6) under field condition were determined using icp-ms. in bt cotton seeds, 54 kinds of inorganic elements were identified by icp-ms, and 5 kinds of major elements, k, p, mg, ca and na, were in range from 138.3 to 13,835.1 microg x g(-1). the contents o ...200919626916
conserved mirna analysis in gossypium hirsutum through small rna sequencing.several mirna family and their targets in cotton had been identified by computational methods based on the conserved characterization of mirnas. so far, there are no experiments to validate the existence of mirnas in cotton. in this study, to analyze the mirnas in cotton, a small rna library of sequences from 18 to 26 nt of gossypium hirsutum seedling has been built by high-throughput sequencing. in this library, 34 conserved mirna families were identified by homology search and the mirna sequen ...200919628031
functional analysis of a cotton cellulose synthase a4 gene promoter in transgenic tobacco plants.a 1,482-bp promoter sequence of the cotton cellulose synthase gene (ghcesa4) was isolated from chinese cultivar cri12 of gossypium hirsutum, and transcriptionally fused to a beta-glucuronidase (gus) reporter gene for investigation of important regions controlling gene expression in transgenic tobacco plants. histochemical staining showed that the full-length promoter directs efficient expression of the reporter gene in the roots, hypocotyls, vascular tissues of stems, trichomes, the central leaf ...200919655147
heat stress-induced limitations to reproductive success in gossypium hirsutum.using in vitro systems, numerous authors have cited the sensitivity of pollen tube growth to high temperature as a major cause of low yields for crops with valuable reproductive structures. we investigated the hypothesis that in vivo fertilization efficiency would be negatively affected by heat stress-induced changes in energy reserves and calcium-mediated oxidative status in the pistil. gossypium hirsutum plants exposed to optimal (30/20 degrees c) or high day temperature (38/20 degrees c) cond ...200919656331
spatiotemporal patterns and dispersal of stink bugs (heteroptera: pentatomidae) in peanut-cotton farmscapes.in the southeast united states, a field of peanuts, arachis hypogaea l., is often closely associated with a field of cotton, gossypium hirsutum l. the objective of this 4-yr on-farm study was to examine and compare the spatiotemporal patterns and dispersal of the southern green stink bug, nezara viridula l., and the brown stink bug, euschistus servus (say), in six of these peanut-cotton farmscapes. gs(+) version 9 was used to generate interpolated estimates of stink bug density by inverse distan ...200919689882
involvement of extracellular cu/zn superoxide dismutase in cotton fiber primary and secondary cell wall biosynthesis.extracellular cu/zn superoxide dismutases (csds) that catalyze the conversion of superoxide to hydrogen peroxide have been suggested to be involved in lignification of secondary walls in spinach, pine and aspen. in cotton fibers, hydrogen peroxide was proposed to be involved in the induction of secondary cell wall biosynthesis. recently, we identified extracellular csds from developing cotton fibers using both immunological and epitope tagging techniques. since cotton fibers are not lignified, w ...200819704453
the ascorbate peroxidase regulated by h(2)o(2) and ethylene is involved in cotton fiber cell elongation by modulating ros homeostasis.ascorbate peroxidase (apx) is a reactive oxygen species (ross) scavenging enzyme involved in regulation of intracellular ros levels by reduction of h(2)o(2) to water using ascorbate as an electron donor. in new phytologist 2007; 175:462-71, we identified a cotton cytosolic apx1 (ghapx1) that was significantly accumulated during the fast fiber-cell elongation period, through a proteomics approach. both the transcript levels of ghapx1 and the total apx activity were highly induced in response to i ...200819704716
efficacy of cry1ac:cry1f proteins in cotton leaf tissue against fall armyworm, beet armyworm, and soybean looper (lepidoptera: noctuidae).cotton, gossypium hirsutum l., plants expressing cry1ac and cry1f insecticidal crystal proteins of bacillus thuringiensis berliner (bt) were evaluated against selected lepidopteran pests including fall armyworm, spodoptera frugiperda (j. e. smith), beet armyworm, spodoptera exigua (hübner), and soybean looper, pseudoplusia includens (walker). studies were conducted in a range of environments, challenging various cotton tissue types from several varieties containing a combination of cry1ac and cr ...200919736762
crop juxtaposition affects cotton fiber quality in georgia farmscapes.phytophagous stink bugs (hemiptera: pentatomidae), including green stink bug [acrosternum hilare (say)], southern green stink bug [nezara viridula (l.)], and brown stink bug [euschistus servus (say)], have become a serious production issue for southeastern u.s. cotton, gossypium hirsutum l., growers. to investigate how different agronomic crops may affect stink bug damage and fiber quality in neighboring cotton fields, replicated 1.6-2.0-ha trials were planted with corn (zea mays l.), peanut (ar ...200919736764
evaluation of transgenic soybean exhibiting high expression of a synthetic bacillus thuringiensis cry1a transgene for suppressing lepidopteran population densities and crop injury.several transgenic lines of soybean, glycine max (l.) merr., expressing a synthetic cry1a gene from bacillus thuringiensis (bt), were examined in replicated field trials in 2003-2007 for suppression of naturally occurring population densities of lepidopteran pests and the resultant crop injury that they caused. bt soybean and negative controls (isogenic segregants and parental lines) were evaluated against velvetbean caterpillar, anticarsia gemmatalis (hübner); soybean looper, pseudoplusia inclu ...200919736779
efficient production of gossypol from hairy root cultures of cotton (gossypium hirsutum l.).a protocol for induction and establishment of agrobacterium rhizogenes mediated hairy root culture of gossypium hirsutum was developed through infection with the a4 strain and co-cultivation on hormone-free semi-solid ms medium with b5 vitamins. it resulted in the emergence of hairy roots from the leaf explants, 21 days after infection. the transformation of hairy roots was established by pcr amplification of rol b and rol c genes of the ri plasmid. all root lines expressed gossypol, although di ...200919751178
enrichment of a set of micrornas during the cotton fiber development.cotton (gossypium hirsutum) is one of the most important economic crops and provides excellent fibers for textile manufacture. in addition to its industrial and agricultural importance, the fiber cell (plant trichome) also is a biological model system for exploring gene expression and regulation. small rnas regulate many aspects of plant growth and development. however, whether small rnas are involved in regulation of fiber cell development is unknown.200919788742
sampling nucleotide diversity in cotton.cultivated cotton is an annual fiber crop derived mainly from two perennial species, gossypium hirsutum l. or upland cotton, and g. barbadense l., extra long-staple fiber pima or egyptian cotton. these two cultivated species are among five allotetraploid species presumably derived monophyletically between g. arboreum and g. raimondii. genomic-based approaches have been hindered by the limited variation within species. yet, population-based methods are being used for genome-wide introgression of ...200919840401
gossypium arboreum ghsp26 enhances drought tolerance in gossypium hirsutum.heat-shock proteins (hsp) are molecular chaperones for protein molecules. these proteins play an important role in protein-protein interactions such as, folding and assisting in the establishment of proper protein conformation and prevention of unwanted protein aggregation. a small hsp gene ghsp26 present in gossypium arboreum responds to dehydration. in the present study, an attempt was made to overcome the problem of drought stress in cotton. a cdna of ghsp26 was isolated from g. arboreum, clo ...201119847887
fifty years of the integrated control concept: the role of landscape ecology in ipm in san joaquin valley cotton.in defining the integrated control concept, stern, smith, van den bosch and hagan described 'understanding the ecosystem' as a key underpinning of the concept. in following years, stern and van den bosch continued to refine and expand the role of the ecological landscape. they and their colleagues developed cultural practices that took advantage of this understanding to limit the need of pesticide intervention in cotton in the san joaquin valley during the 1960s and 1970s. research and extension ...200919856340
clustering, haplotype diversity and locations of mic-3: a unique root-specific defense-related gene family in upland cotton (gossypium hirsutum l.).mic-3 is a recently identified gene family shown to exhibit increased root-specific expression following nematode infection of cotton plants that are resistant to root-knot nematode. here, we cloned and sequenced mic-3 genes from selected diploid and tetraploid cotton species to reveal sequence differences at the molecular level and identify chromosomal locations of mic-3 genes in gossypium species. detailed sequence analysis and phylogenetic clustering of mic-3 genes indicated the presence of m ...201019862497
the ultrastructure of cotyledonary tissue from gossypium hirsutum l. seeds.quiescent cottonseeds stored in a dry, anaerobic situation for over a year have been shown to contain cells whose contents are ultrastructurally similar to those of normal, fully hydrated plant cells. plastids, mitochondria, and nuclei of the cells of cotyledon tissue in dry seeds possess normal-looking double membranes even under conditions of extreme desiccation. previous reports have indicated on the basis of light microscopic work, that the cells of certain dry seeds do not possess nuclear m ...196519866664
characterization of two nadph: cytochrome p450 reductases from cotton (gossypium hirsutum).cytochrome p450 monooxygenases (p450s) are commonly involved in biosynthesis of endogenous compounds and catabolism of xenobiotics, and their activities rely on a partner enzyme, cytochrome p450 reductase (cpr, e.c.1.6.2.4). two cpr cdnas, ghcpr1 and ghcpr2, were isolated from cotton (gossypium hirsutum). they are 71% identical to each other at the amino acid sequence level and belong to the class i and ii of dicotyledonous cprs, respectively. the recombinant enzymes reduced cytochrome c, ferric ...201019883924
molecular cloning and localization of a novel cotton annexin gene expressed preferentially during fiber development.annexins constitute a family of multifunction and structurally related proteins. these proteins are ubiquitous in the plant kingdom, and are important calcium-dependent membrane-binding proteins that participate in the polar development of different plant regions such as rhizoids, root caps, and pollen tube tips. in this study, a novel cotton annexin gene (designated as ghfannx) was isolated from a fiber cdna library of cotton (gossypium hirsutum). the full-length cdna of ghfannx comprises an op ...201019885739
tarnished plant bug (hemiptera: miridae) thresholds and sampling comparisons for flowering cotton in the midsouthern united states.the tarnished plant bug, lygus lineolaris (palisot de beauvois) (hemiptera: miridae), has become the primary target of foliar insecticides in cotton, gossypium hirsutum l., throughout the midsouth over the past several years. this prompted a reevaluation of existing action thresholds for flowering cotton under current production practices and economics. a trial was conducted at 19 locations throughout the midsouth during 2006 and 2007. threshold treatments ranged from a weekly automatic insectic ...200919886447
variation in resistance to pyrethroids in helicoverpa armigera from benin republic, west africa.pyrethroid resistance in helicoverpa armigera (hübner) field populations was investigated in benin over several years by using third- and fourth-instar larval topicalbioassays. h. armigera was resistant to pyrethroids tested as cypermethrin, deltamethrin, bifenthrin, and fenvalerate. pretreatment with piperonyl butoxide significantly decreased the ld50 value of cypermethrin and deltamethrin, and the resistance suppression by this synergist effect was observed. no significant decrease in the ld50 ...200919886459
genome-wide analysis reveals rapid and dynamic changes in mirna and sirna sequence and expression during ovule and fiber development in allotetraploid cotton (gossypium hirsutum l.).cotton fiber development undergoes rapid and dynamic changes in a single cell type, from fiber initiation, elongation, primary and secondary wall biosynthesis, to fiber maturation. previous studies showed that cotton genes encoding putative myb transcription factors and phytohormone responsive factors were induced during early stages of ovule and fiber development. many of these factors are targets of micrornas (mirnas) that mediate target gene regulation by mrna degradation or translational rep ...200919889219
global gene expression responses to waterlogging in roots and leaves of cotton (gossypium hirsutum l.).waterlogging stress causes yield reduction in cotton (gossypium hirsutum l.). a major component of waterlogging stress is the lack of oxygen available to submerged tissues. while changes in expressed protein, gene transcription and metabolite levels have been studied in response to low oxygen stress, little research has been done on molecular responses to waterlogging in cotton. we assessed cotton growth responses to waterlogging and assayed global gene transcription responses in root and leaf c ...201019923201
effect of h2o2 on fiber initiation using fiber retardation initiation mutants in cotton (gossypium hirsutum).single-celled fibers initiate at anthesis from cotton seed epidermal cells of normal developmental cotton cultivars; however, fiber initiation is retarded in some cotton fiber mutants. in this study, the relationship between genes associated with fiber initiation retardation and fiber initiation development was investigated using three cotton fiber developmental mutants: recessive naked seed n2; dominant naked seed n1; and xinxiangxiaoji linted-fuzzless mutant (xinflm); with genetic standard lin ...201019931935
response of the enzymes to nitrogen applications in cotton fiber (gossypium hirsutum l.) and their relationships with fiber strength.to investigate the response of key enzymes to nitrogen (n) rates in cotton fiber and its relationship with fiber strength, experiments were conducted in 2005 and 2006 with cotton cultivars in nanjing. three n rates 0, 240 and 480 kgn/hm(2), signifying optimum and excessive nitrogen application levels were applied. the activities and the gene expressions of the key enzymes were affected by n, and the characteristics of cellulose accumulation and fiber strength changed as the n rate varied. beta-1 ...200919937205
proteomic identification of differentially expressed proteins in the ligon lintless mutant of upland cotton (gossypium hirsutum l.).cotton fiber is an ideal model for studying plant cell elongation. to date, the underlying mechanisms controlling fiber elongation remain unclear due to their high complexity. in this study, a comparative proteomic analysis between a short-lint fiber mutant (ligon lintless, li(1)) and its wild-type was performed to identify fiber elongation-related proteins. by 2-de combined with local est database-assisted ms/ms analysis, 81 differentially expressed proteins assigned to different functional cat ...201019954254
a new snp haplotype associated with blue disease resistance gene in cotton (gossypium hirsutum l.).resistance to cotton blue disease (cbd) was evaluated in 364 f(2.3) families of three populations derived from resistant variety 'delta opal'. the cbd resistance in 'delta opal' was controlled by one single dominant gene designated cbd. two simple sequence repeat (ssr) markers were identified as linked to cbd by bulked segregant analysis. cbd resides at the telomere region of chromosome 10. ssr marker dc20027 was 0.75 cm away from cbd. dc20027 marker fragments amplified from 3 diploid species an ...201019960336
genotypic differences in thermotolerance are dependent upon prestress capacity for antioxidant protection of the photosynthetic apparatus in gossypium hirsutum.numerous studies have illustrated the need for antioxidant enzymes in acquired photosynthetic thermotolerance, but information on their possible role in promoting innate thermotolerance is lacking. we investigated the hypothesis that genotypic differences in source leaf photosynthetic thermostability would be dependent upon prestress capacity for antioxidant protection of the photosynthetic apparatus in gossypium hirsutum. to test this hypothesis, thermosensitive (cv. st4554) and reportedly ther ...201020002327
apyrase (nucleoside triphosphate-diphosphohydrolase) and extracellular nucleotides regulate cotton fiber elongation in cultured ovules.ectoapyrase enzymes remove the terminal phosphate from extracellular nucleoside tri- and diphosphates. in arabidopsis (arabidopsis thaliana), two ectoapyrases, atapy1 and atapy2, have been implicated as key modulators of growth. in fibers of cotton (gossypium hirsutum), transcript levels for ghapy1 and ghapy2, two closely related ectoapyrases that have high sequence similarity to atapy1 and atapy2, are up-regulated when fibers enter their rapid growth phase. in an ovule culture system, fibers re ...201020018604
long-term effects of land application of class b biosolids on the soil microbial populations, pathogens, and activity.this study evaluated the influence of 20 annual land applications of class b biosolids on the soil microbial community. the potential benefits and hazards of land application were evaluated by analysis of surface soil samples collected following the 20th land application of biosolids. the study was initiated in 1986 at the university of arizona marana agricultural center, 21 miles north of tucson, az. the final application of biosolids was in march 2005, followed by growth of cotton (gossypium h ...201020048328
cloning and characterization of the gossypium hirsutum major latex protein gene and functional analysis in arabidopsis thaliana.the major latex protein (mlp) gene in gossypium hirsutum was cloned and designated gh-mlp. expression in cotton root was induced by salt stress and verticillium dahliae toxin, and bioinformatic analysis showed that gh-mlp encodes a 157-amino acid protein that is similar to members of the mlp subfamily in the bet v 1 family. although the structure of mlp is similar to bet v 1 family proteins, the sequence identity to other subfamilies of bet v 1 proteins is less than 20%. the gh-mlp promoter cont ...201020049612
fusarium verticillioides (saccardo) nirenberg associated with hardlock of cotton.boll rots of cotton (gossypium hirsutum l.) are common in the humid areas of the southeastern us. one type of boll damage that may be differentiated from others is hardlock, with symptoms that include compression of the fibers within individual locules of mature, open cotton bolls without further obvious disintegration of the lint or damage to the carpel wall. the principal economic effect is that the boll's lint is unharvestable by mechanical cotton pickers. this disease is endemic to the south ...201020052584
tarnished plant bug (hemiptera: miridae) thresholds for cotton before bloom in the midsouth of the united states.insecticide applications to control tarnished plant bug, lygus lineolaris (palisot de beauvois) (hemiptera: miridae), during cotton, gossypium hirsutum l., bud formation are common throughout the midsouth of the united states. cultivation practices and the pest complex have changed since action thresholds were established for this pest. a trial was conducted at 33 locations over 3 yr throughout the midsouth to evaluate tarnished plant bug damage to cotton during the prebloom period. there was no ...200920069839
susceptibility of helicoverpa armigera (lepidoptera: noctuidae) to cry1ac and cry2ab2 insecticidal proteins in four countries of the west african cotton belt.transgenic cotton, gossypium hirsutum l., producing cry1ac and cry2ab2 insecticidal proteins from bacillus thuringiensis (bt) was first planted in burkina faso (west africa) in 2008. here, we provide the first baseline data on susceptibility of helicoverpa armigera (hübner) (lepidoptera: noctuidae) populations collected in west africa (benin, cameroon, nigeria, and chad) to cry1ac and cry2ab2 toxins incorporated separately or in combination in a semisynthetic diet. for populations collected in 2 ...200920069861
sampling stink bugs (hemiptera: pentatomidae) for population estimation and pest management in southeastern cotton production.stink bugs (hemiptera: pentatomidae) were sampled in commercial cotton, gossypium hirsutum l., fields in 2007 and 2008 in south carolina and georgia. the main species collected with the beat cloth and sweep net methods were green stink bug, acrosternum hilare (say) (63 and 57%, respectively); brown stink bug, euschistus servus (say) (23 and 18%, respectively); and southern green stink bug, nezara viridula (l.) (14 and 22%, respectively). average stink bug densities were 0.145 +/- 0.010 (mean +/- ...200920069868
cotton chromosome substitution lines crossed with cultivars: genetic model evaluation and seed trait analyses.seed from upland cotton, gossypium hirsutum l., provides a desirable and important nutrition profile. in this study, several seed traits (protein content, oil content, seed hull fiber content, seed index, seed volume, embryo percentage) for f(3) hybrids of 13 cotton chromosome substitution lines crossed with five elite cultivars over four environments were evaluated. oil and protein were expressed both as percentage of total seed weight and as an index which is the grams of product/100 seeds. an ...201020087566
near-isogenic cotton germplasm lines that differ in fiber-bundle strength have temporal differences in fiber gene expression patterns as revealed by comparative high-throughput profiling.gene expression profiles of developing cotton (gossypium hirsutum l.) fibers from two near-isogenic lines (nils) that differ in fiber-bundle strength, short-fiber content, and in fewer than two genetic loci were compared using an oligonucleotide microarray. fiber gene expression was compared at five time points spanning fiber elongation and secondary cell wall (scw) biosynthesis. fiber samples were collected from field plots in a randomized, complete block design, with three spatially distinct b ...201020087569
an evaluation of eco-friendly naturally coloured cottons regarding seed cotton yield, yield components and major lint quality traits under conditions of east mediterranean region of turkey.in the study carried out in 2002-2003 in the east mediterranean region of turkey (in kahramanmaras province), four different naturally coloured cotton (gossypium hirsutum l.) (dark brown, light brown, cream and green) lines from azerbaijan and two white linted cotton varieties (maras-92 and sayar-314 (g. hirsutum l.)) of the region were used as material. the aim of this study was to determine seed cotton yield and yield components and major lint quality traits of investigated coloured cotton lin ...200920128501
the essential role of ghpel gene, encoding a pectate lyase, in cell wall loosening by depolymerization of the de-esterified pectin during fiber elongation in cotton.cotton fiber elongation, largely achieved by cell wall loosening, is an important stage during cotton fiber development. in this present research, a fiber preferential cdna encoding a pectate lyase (pel) which could exclusively degrade the de-esterified pectin was isolated from a cotton (gossypium hirsutum) fiber cdna library. subsequently, the corresponding pel genes were isolated from four different cotton species and characterized. in vitro enzyme assays indicated that ghpel really exhibited ...201020131110
characterization of an adp-glucose pyrophosphorylase small subunit gene expressed in developing cotton (gossypium hirsutum) fibers.adp-glucose pyrophosphorylase (adpgp, ec 2.7.7.27) is a tetrameric protein composed of two small and two large subunits that catalyzes the biosynthesis of adp-glucose from glucose-phosphate which is used to provide the glucose subunits for starch biosynthesis. a second cotton gene encoding an adpgp small subunit has been cloned and characterized. the gene contains eight introns similar to previously reported potato and cotton adpgp small subunit genes. the deduced translation of the gene contain ...201120140709
methodological evaluation of 2-de to study root proteomics during nematode infection in cotton and coffee plants.the identification of plant proteins expressed in response to phytopathogens is a remaining challenge to proteome methodology. proteomic methods, such as electrophoresis and mass spectrometry have been extensively used for protein differential expression studies in several plants including arabidopsis thaliana, rice, and wheat. however, in coffee (coffea canephora) and cotton (gossypium hirsutum), bidimensional electrophoresis (2-de) analysis has been rarely employed. moreover, global protein ex ...201020213576
ghwrky3, a novel cotton (gossypium hirsutum l.) wrky gene, is involved in diverse stress responses.wrky proteins play important roles in plant defense responses. in the present study, a novel wrky gene, nominated as ghwrky3, was isolated from cotton (gossypium hirsutum l.). the full-length cdna of ghwrky3 is 1,705 bp in length and encodes a protein with 507 amino acids containing two typical wrky domains and two zinc finger motifs. amino acid sequence alignment revealed that ghwrky3 shares a high degree of identity with other higher plant wrky proteins. the subcellular localization assay indi ...201120238169
characterization of two cotton (gossypium hirsutum l) invertase genes.two cotton vacuolar-invertase genes were identified and sequenced. both genes had seven exons, including an unusually small second exon typical of acid invertases. these genes encode peptides with many features shared by acid invertases from other species including, leader sequences that probably target the peptide to the vacuole, active site motifs and substrate binding motifs. expression analyses indicated that one of the genes was expressed in roots during the starch filling stage of developm ...201020300865
identification and evaluation of new reference genes in gossypium hirsutum for accurate normalization of real-time quantitative rt-pcr data.normalizing through reference genes, or housekeeping genes, can make more accurate and reliable results from reverse transcription real-time quantitative polymerase chain reaction (qpcr). recent studies have shown that no single housekeeping gene is universal for all experiments. thus, suitable reference genes should be the first step of any qpcr analysis. only a few studies on the identification of housekeeping gene have been carried on plants. therefore qpcr studies on important crops such as ...201020302670
effects of vegetated field borders on arthropods in cotton fields in eastern north carolina.the influence, if any, of 5m wide, feral, herbaceous field borders on pest and beneficial arthropods in commercial cotton, gossypium hirsutum (l.) (malvales: malvaceae), fields was measured through a variety of sampling techniques over three years. in each year, 5 fields with managed, feral vegetation borders and five fields without such borders were examined. sampling was stratified from the field border or edge in each field in an attempt to elucidate any edge effects that might have occurred. ...200820345293
a new cotton sdr family gene encodes a polypeptide possessing aldehyde reductase and 3-ketoacyl-coa reductase activities.to understand regulatory mechanisms of cotton fiber development, microarray analysis has been performed for upland cotton (gossypium hirsutum). based on this, a cdna (ghkcr3) encoding a polypeptide belonging to short-chain alcohol dehydrogenase/reductase family was isolated and cloned. it contains an open reading frame of 987 bp encoding a polypeptide of 328 amino acid residues. following its overexpression in bacterial cells, the purified recombinant protein specifically uses nadph to reduce a ...201020370610
genetic transformation of cotton with a harpin-encoding gene hpaxoo confers an enhanced defense response against different pathogens through a priming mechanism.the soil-borne fungal pathogen verticillium dahliae kleb causes verticillium wilt in a wide range of crops including cotton (gossypium hirsutum). to date, most upland cotton varieties are susceptible to v. dahliae and the breeding for cotton varieties with the resistance to verticillium wilt has not been successful.201020398293
development of sampling plans for cotton bolls injured by stink bugs (hemiptera: pentatomidae).cotton, gossypium hirsutum l., bolls were sampled in commercial fields for stink bug (hemiptera: pentatomidae) injury during 2007 and 2008 in south carolina and georgia. across both years of this study, boll-injury percentages averaged 14.8 +/- 0.3 (sem). at average boll injury treatment levels of 10, 20, 30, and 50%, the percentage of samples with at least one injured boll was 82, 97, 100, and 100%, respectively. percentage of field-sampling date combinations with average injury < 10, 20, 30, a ...201020429470
bcg vaccination in the cotton rat (sigmodon hispidus) infected by the pulmonary route with virulent mycobacterium tuberculosis.to evaluate the usefulness of the american cotton rat (sigmodon hispidus) in the evaluation of vaccine-induced resistance, we infected bcg-vaccinated and non-vaccinated cotton rats with mycobacterium tuberculosis (h37rv) via the respiratory route. lung histopathology of these animals showed loose, disorganized granulomas which were non-necrotic up to 8 weeks post-infection. moreover, we were not able to detect a dth response after intradermal injection with ppd antigen. prior bcg vaccination sig ...201020451457
cold acclimation and low temperature resistance in cotton: gossypium hirsutum phospholipase dalpha isoforms are differentially regulated by temperature and light.phospholipase dalpha (pldalpha) was isolated from cultivated cotton (gossypium hirsutum) and characterized. two pldalpha genes were identified in the allotetraploid genome of g. hirsutum, derived from its diploid progenitors, g. raimondii and g. arboreum. the genes contained three exons and two introns. the translated products shared a 98.6% homology and were designated as grpldalpha and gapldalpha. their orfs encoded a polypeptide of 807 amino acids with a predicted molecular mass of 91.6 kda s ...201020478966
[determination of protein and gossypol content in cotton kernel powder with near infrared reflectance spectroscopy].near-infrared reflectance spectroscopy (nirs) was used as a rapid and nondestructive method to determine the protein content and gossypol content in cotton kernel powder samples, using 49 upland cotton (gossypium hirsutum l.) germplasms and 188 recombinant inbred lines (rils). the cottonseed samples harvested from the upland cotton germplasms and rils grown in different cotton growing regions in different years were analyzed chemically for protein and gossypol contents, as well as scanned in the ...201020496676
fusarium wilt (fusarium oxysporum f. sp. vasinfectum) genes expressed during infection of cotton (gossypium hirsutum)dagger.summary we sought to identify fusarium oxysporum f. sp. vasinfectum (fov) genes that may be associated with pathogenicity. initially we utilized microarray and q-pcr technology to identify fov genes expressed in root and hypocotyl tissues during a compatible infection of cotton. we identified 218 fungal clones representing 174 fov non-redundant genes as expressed in planta. the majority of the expressed sequences were expressed in infected roots, with only six genes detected in hypocotyl tissue. ...200620507430
molecular characterization and experimental host-range of two begomoviruses infecting clerodendrum cyrtophyllum in china.two begomovirus isolates (yx2-i and yx2-ii) were identified from clerodendrum cyrtophyllum showing yellow mosaic symptoms collected in jiangsu province of china. sequence analysis reveals that yx2-i is a distinct begomovirus species for which the name clerodendrum golden mosaic jiangsu virus (clgmjsv-[cn:yx2:08]) is proposed. yx2-ii is an isolate of bipartite begomovirus clerodendrum golden mosaic china virus (clgmcnv-[cn:yx2:08]). infectious clones of the two viruses were constructed and agroin ...201020532974
spatial dynamics of stink bugs (hemiptera: pentatomidae) and associated boll injury in southeastern cotton fields.grid sampling (one sample per 0.40 ha) in 12 cotton (gossypium hirsutum l.) fields for stink bugs and boll injury caused by stink bug feeding was conducted in 2007 and 2008 in south carolina and georgia. the main species collected using the beat cloth method were the green stink bug, acrosternum hilare (say) (69%); brown stink bug, euschistus servus (say) (18%); and the southern green stink bug, nezara viridula (l.) (14%), respectively. the inverted distance weighted interpolation method and spa ...201020550811
overexpression of a profilin (ghpfn2) promotes the progression of developmental phases in cotton fibers.cotton fiber development at the stages of elongation and secondary wall synthesis determines the traits of fiber length and strength. to date, the mechanisms controlling the progression of these two phases remain elusive. in this work, the function of a fiber-preferential actin-binding protein (ghpfn2) was characterized by cytological and molecular studies on the fibers of transgenic green-colored cotton (gossypium hirsutum) through three successive generations. overexpression of ghpfn2 caused p ...201020558432
a draft physical map of a d-genome cotton species (gossypium raimondii).genetically anchored physical maps of large eukaryotic genomes have proven useful both for their intrinsic merit and as an adjunct to genome sequencing. cultivated tetraploid cottons, gossypium hirsutum and g. barbadense, share a common ancestor formed by a merger of the a and d genomes about 1-2 million years ago. toward the long-term goal of characterizing the spectrum of diversity among cotton genomes, the worldwide cotton community has prioritized the d genome progenitor gossypium raimondii ...201020569427
meta-analysis of cotton fiber quality qtls across diverse environments in a gossypium hirsutum x g. barbadense ril population.cotton fibers (produced by gossypium species) are the premier natural fibers for textile production. the two tetraploid species, g. barbadense (gb) and g. hirsutum (gh), differ significantly in their fiber properties, the former having much longer, finer and stronger fibers that are highly prized. a better understanding of the genetics and underlying biological causes of these differences will aid further improvement of cotton quality through breeding and biotechnology. we evaluated an inter-spe ...201020584292
ssr markers closely associated with genes for resistance to root-knot nematode on chromosomes 11 and 14 of upland cotton.molecular markers closely linked to genes that confer a high level of resistance to root-knot nematode (rkn) [meloidogyne incognita (kofoid & white) chitwood] in cotton (gossypium hirsutum l.) germplasm derived from auburn 623 rnr would greatly facilitate cotton breeding programs. our objectives were to identify simple sequence repeat (ssr) markers linked to rkn resistance quantitative trait loci (qtl) and map these markers to specific chromosomes. we developed three recombinant inbred line (ril ...201020607210
identification of the family of aquaporin genes and their expression in upland cotton (gossypium hirsutum l.).cotton (gossypium spp.) is produced in over 30 countries and represents the most important natural fiber in the world. one of the primary factors affecting both the quantity and quality of cotton production is water. a major facilitator of water movement through cell membranes of cotton and other plants are the aquaporin proteins. aquaporin proteins are present as diverse forms in plants, where they function as transport systems for water and other small molecules. the plant aquaporins belong to ...201020626869
fine mapping qmi-c11 a major qtl controlling root-knot nematodes resistance in upland cotton.the identification and utilization of a high-level of host plant resistance is the most effective and economical approach to control root-knot nematode (meloidogyne incognita). in an earlier study, we identified a major quantitative trait locus (qtl) for resistance to root-knot nematode in the m-120 rnr upland cotton line (gossypium hirsutum l.) of the auburn 623 rnr source. the qtl is located in a 12.9-cm interval flanked by the two ssr markers cir069 and cir316 on the distal segment of chromos ...201020680610
geostatistical modeling of the spatial variability and risk areas of southern root-knot nematodes in relation to soil properties.identifying the spatial variability and risk areas for southern root-knot nematode [meloidogyne incognita (kofoid & white) chitwood] (rkn) is key for site-specific management (ssm) of cotton (gossypium hirsutum l.) fields. the objectives of this study were to: (i) determine the soil properties that influence rkn occurrence at different scales; and (ii) delineate risk areas of rkn by indicator kriging. the study site was a cotton field located in the southeastern coastal plain region of the usa. ...201020717481
comparisons of early transcriptome responses to low-oxygen environments in three dicotyledonous plant species.waterlogging is a serious impediment to crop productivity worldwide which acts to reduce oxygen levels in the rhizosphere due to the low diffusion rate of molecular oxygen in water. plants respond to low oxygen through rapid and specific changes at both the transcriptional and translational levels. transcriptional changes to low-oxygen (hypoxia) stress have been studied in a number of plant species using whole genome microarrays. using transcriptome data from root tissue from early time points ( ...201020724824
the history and disposition of transposable elements in polyploid gossypium.transposable elements (tes) are a major component of plant genomes. it is of particular interest to explore the potential activation of te proliferation, especially in hybrids and polyploids, which often are associated with rapid genomic and epigenetic restructuring. here we explore the consequences of genomic merger and doubling on copia and gypsy-like gorge3 long terminal repeat (ltr) retrotransposons as well as on non-ltr long interspersed nuclear elements (lines) in allotetraploid cotton, go ...201020725147
transcriptome profiling of early developing cotton fiber by deep-sequencing reveals significantly differential expression of genes in a fuzzless/lintless mutant.cotton fiber as a single-celled trichome is a biological model system for studying cell differentiation and elongation. however, the complexity of its gene expression and regulatory mechanism allows only marginal progress. here, we report the high-throughput tag-sequencing (tag-seq) analysis using solexa genome analyzer platform on transcriptome of -2 to 1 (fiber initiation, stage i) and 2-8 (fiber elongation, stage ii) days post anthesis (dpa) cotton (gossypium hirsutum) ovules (wild type: wt; ...201020828606
diurnal pollen tube growth rate is slowed by high temperature in field-grown gossypium hirsutum pistils.for gossypium hirsutum pollination, germination, and pollen tube growth must occur in a highly concerted fashion on the day of flowering for fertilization to occur. because reproductive success could be influenced by the photosynthetic activity of major source leaves, we hypothesized that increased temperatures under field conditions would limit fertilization by inhibiting diurnal pollen tube growth through the style and decreasing subtending leaf photosynthesis. to address this hypothesis, g. h ...201120832140
fate and efficacy of metolachlor granular and emulsifiable concentrate formulations in a conservation tillage system.use of genetically modified cultivars resistant to the herbicide glyphosate (n-phosphonomethylglycine) is strongly associated with conservation-tillage (cst) management for maize ( zea mays l.), soybean ( glycine max l.), and cotton ( gossypium hirsutum l.) cultivation. due to the emergence of glyphosate-resistant weed biotypes, alternate weed management practices are needed to sustain cst use. this work focused on metolachlor use (2-chloro-n-(2-ethyl-6-methylphenyl)-n-(2-methoxy-1-methylethyl)a ...201020836497
two cotton cys2/his2-type zinc-finger proteins, ghdi19-1 and ghdi19-2, are involved in plant response to salt/drought stress and abscisic acid signaling.cotton (gossypium hirsutum) often encounters abiotic stress such as drought and high salinity during its development, and its productivity is significantly limited by those adverse factors. to investigate the molecular adaptation mechanisms of this plant species to abiotic stress, we identified two genes encoding di19-like cys2/his2 zinc-finger proteins in cotton. gfp fluorescence assay demonstrated that ghdi19-1 and ghdi19-2 are two nuclear-localized proteins. quantitative rt-pcr and northern b ...201020852918
potential impact of differential production of the cry2ab and cry1ac proteins in transgenic cotton in response to cold stress.transgenic bollgard ii cotton, gossypium hirsutum l., expresses cry1ac and cry2ab proteins that provide control of lepidopteran larvae, including helicoverpa and heliothis species (lepidoptera: noctuidae) worldwide. experiments conducted at katherine, northern territory, australia evaluated the impact of night minimum temperatures on cry1ac and cry2ab protein levels in bollgard ii cotton. in both 2003 and 2004, potted plants were either grown outside continuously or protected from cold in a glas ...201020857729
association of aflp and ssr markers with agronomic and fibre quality traits in gossypium hirsutum l.molecular markers linked to qtl contributing to agronomic and fibre quality traits would be useful for cotton improvement. we have attempted to tag yield and fibre quality traits with aflp and ssr markers using f(2) and f(3) populations of a cross between two gossypium hirsutum varieties, ps56-4 and rs2013. out of 50 aflp primer combinations and 177 ssr primer pairs tested, 32 aflp and four ssr primers were chosen for genotyping f(2) individuals. marker-trait associations were studied for eight ...201020861566
[photosynthetic rate, transpiration rate, and water use efficiency of cotton canopy in oasis edge of linze].the measurement system of li-8100 carbon flux and the modified assimilation chamber were used to study the photosynthetic characteristics of cotton (gossypium hirsutum l.) canopy in the oasis edge region in middle reach of heihe river basin, mid hexi corridor of gansu. at the experimental site, soil respiration and evaporation rates were significantly higher in late june than in early august, and the diurnal variation of canopy photosynthetic rate showed single-peak type. the photosynthetic rate ...201020873616
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