Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| modifications of dubos's media for the cultivation of mycobacterium johnei. | 1953 | 13118442 | |
| the effect of various chemical agents on the viability of mycobacterium paratuberculosis in vitro. | 1954 | 13124698 | |
| [curative action of isonicotinic acid hydrazide (inh) on infection induced in the mouse by strain 607 of mycobacterium paratuberculosis]. | 1953 | 13124955 | |
| the effect of chemotherapeutic agents on mycobacterium johnei. | 1954 | 13130715 | |
| experimental infection with mycobacterium johnei. i. the effect of a low calcium diet on the disease in goats. | 1954 | 13163243 | |
| source of the phlei growth factor for mycobacterium johnei. | 1955 | 13223788 | |
| isolation of mycobacterium johnei from faeces. | 1956 | 13307351 | |
| [effect of isonicotinic acid hydrazide on mycobacteria. i. effect on the enzyme system and chemical structure of the bacillus paratuberculosis grassberger 55]. | 1955 | 13324598 | |
| [manometric methods of evaluating the glucide metabolism of mycobacterium tuberculosis var. avium and mycobacterium paratuberculosis]. | 1956 | 13337028 | |
| survival time of mycobacterium paratuberculosis. | 1956 | 13340126 | |
| infection of hamsters and rabbits with mycobacterium johnei. | 1956 | 13345902 | |
| experimental infection with mycobacterium johnei. ii. the histopathology of infection in experimental goats. | 1957 | 13406122 | |
| the intravenous inoculation of sheep with graded doses of mycobacterium johnei. | 1958 | 13502483 | |
| the chemical constituents of mycobacterium paratuberculosis. | 1958 | 13521267 | |
| a purified complement-fixing antigen from mycobacterium johnei. | 1958 | 13541424 | |
| the experimental infection of cattle with mycobacterium johnei. i. calves inoculated intravenously. | 1958 | 13563683 | |
| the isolation of mycobacterium johnei and other acid-fast bacilli from the retropharyngeal and ileocaecal lymph-glands and spleen of apparently normal cattle. | 1958 | 13576362 | |
| a mycobactin-containing liquid medium for the study of mycobacterium johnei. | 1958 | 13576363 | |
| the estimation of doses of mycobacterium johnei suitable for the production of johne's disease in cattle. | 1959 | 13642211 | |
| greater susceptibility to mycobacterium johnei of c.57 black mice, when compared with cba and swiss white mice. | 1961 | 13692304 | |
| infection of laboratory animals with mycobacterium johnei. i. infection in swiss white mice and its modification by suramin and cortisone. | 1961 | 13692305 | |
| infection of laboratory animals with mycobacterium johnei. ii. infection in white rats; effect of cortisone treatment. | 1961 | 13692306 | |
| infection of laboratory animals with mycobacterium johnei. iii. liver and spleen infections of vaccinated and unvaccinated swiss white mice. | 1961 | 13692307 | |
| infection of laboratory animals with mycobacterium johnei. iv. comparative susceptibility to infection of c.57, c.b.a and swiss white mice. | 1961 | 13692308 | |
| confirmation of a calculated id50 of mycobacterium johnei for the experimental production of johne's disease in cattle. | 1961 | 13739487 | |
| the experimental infection of cattle with mycobacterium johnei. ii. adult cattle inoculated intravenously. | 1961 | 13739488 | |
| the experimental infection of cattle with mycobacterium johnei. iii. calves maintained in an infectious environment. | 1961 | 13739489 | |
| allergy studies on cattle vaccinated with killed mycobacterium paratuberculosis. | 1960 | 13759176 | |
| separation and serologic identification of fractions from the culture filtrate of mycobacterium paratuberculosis. | 1961 | 13769617 | |
| infection of laboratory animals with mycobacterium johnei. v. further studies on the comparative susceptibility of c57 black mice. | 1962 | 13878044 | |
| quantitative studies of mycobacterium johnei in the tissues of sheep. iv. the distribution of m. johnei shortly after oral dosing. | 1962 | 13898699 | |
| [nature of mycolic acids from mycobacterium paratuberculosis; application of thin layer chromatography to their fractionation]. | 1963 | 13928518 | |
| improved methods for primary cultivation of mycobacterium paratuberculosis. | 1962 | 13934998 | |
| the pathogenesis of mycobacterium johnei in orally dosed hamsters. | 1963 | 13947979 | |
| the susceptibility of mycobacterium paratuberculosis (johne's bacillus) to chemotherapeutic agents. | 1964 | 14117693 | |
| use of arthrobacter terregens for bioassay of mycobactin. | reich, claude v. (johns hopkins-leonard wood memorial leprosy research laboratory, johns hopkins university, baltimore, md.), and john h. hanks. use of arthrobacter terregens for bioassay of mycobactin. j. bacteriol. 87:1317-1320. 1964.-arthrobacter terregens was used to assay mycobactin, a growth factor for mycobacterium paratuberculosis. within 7 days, a. terregens gave a linear photometric growth response to mycobactin in the range of 0.05 to 0.2 mug/ml. preparations found to be active (or in ... | 1964 | 14188708 |
| specificity of improved methods for mycobactin bioassay by arthrobacter terregens. | antoine, alan d. (johns hopkins university-leonard wood memorial leprosy research laboratory, baltimore, md.), norman e. morrison, and john h. hanks. specificity of improved methods for mycobactin bioassay by arthrobacter terregens. j. bacteriol. 88:1672-1677. 1964.-arthrobacter terregens was used to assay mycobactin, a growth factor for mycobacterium paratuberculosis. improved techniques permit the assay of mycobactin within 3 to 4 days by agarplate or liquid-medium methods. for the agarplate m ... | 1964 | 14240956 |
| improvements in the techniques for primary cultivation of mycobacterium paratuberculosis. | 1964 | 14266884 | |
| circumvention of the mycobactin requirement of mycobacterium paratuberculosis. | morrison, norman e. (johns hopkins university-leonard wood memorial leprosy research laboratory, baltimore, md.). circumvention of the mycobactin requirement of mycobacterium paratuberculosis. j. bacteriol. 89:762-767. 1965.-the mycobactin growth requirement of mycobacterium paratuberculosis was circumvented on glucose-containing synthetic medium with an initial ph of 5.5. mycobactin was required during the first transfer on the synthetic medium. subsequent transfers have grown in the absence of ... | 1965 | 14273658 |
| utilization of external growth factors by intracellular microbes: mycobacterium paratuberculosis and wood pigeon mycobacteria. | wheeler, william c. (johns hopkins university-leonard wood memorial leprosy research laboratory, baltimore, md.), and john h. hanks. utilization of external growth factors by intracellular microbes: mycobacterium paratuberculosis and wood pigeon mycobacteria. j. bacteriol. 89:889-896. 1965.-the extent to which the intracellular growth of microbes is dependent upon capacities for growth in vitro has been investigated by use of organisms which require a specific factor, mycobactin, for isolation a ... | 1965 | 14273675 |
| studies of immunity to mycobacterium johnei in sheep. | 1965 | 14317616 | |
| media for mycobacterium johnei. | 1965 | 14322175 | |
| the structure of mycobactin p, a growth factor for mycobacterium johnei, and the significance of its iron complex. | 1. the growth factor from mycobacterium phlei is now named mycobactin p to distinguish it from related but chemically distinct growth factors from other species of mycobacteria. it is shown to comprise four closely similar chemical entities differing only in the fatty side chain. 2. the structure of the main component (85%) of mycobactin p has been established and the absolute configuration of all asymmetric centres determined. 3. ferric mycobactin p has been crystallized; its structure is discu ... | 1965 | 14342223 |
| [isolation of peptide lipids from mycobacterium paratuberculosis]. | 1962 | 14462029 | |
| a technique for concentrating mycobacterium paratuberculosis present in intestinal mucosa. | 1961 | 14462488 | |
| quantitative studies of mycobacterium johnei in tissues of sheep. iii. intestinal histopathology. | 1962 | 14479935 | |
| the experimental infection of cattle with mycobacterium johnei. iv. adult cattle maintained in an infectious environment. | 1962 | 14490277 | |
| evaluation of bacteriologic culture of individual and pooled fecal samples for detection of mycobacterium paratuberculosis in dairy cattle herds. | to determine the sensitivity of bacteriologic culture of pooled fecal samples in detecting mycobacterium paratuberculosis, compared with bacteriologic culture of individual fecal samples in dairy cattle herds. | 2003 | 14552493 |
| comparison of histopathology, cultivation of tissues and rectal contents, and interferon-gamma and serum antibody responses for the diagnosis of bovine paratuberculosis. | the diagnosis of infection with mycobacterium avium subsp. paratuberculosis was studied in 12 cattle from infected dairy herds and four from non-infected herds. a comparison was made of (1) histopathological examination and bacterial culture of tissues, (2) culture of serial samples of rectal contents, and (3) examination of repeated blood samples for interferon-gamma (ifn-gamma) and antibody responses. tissue samples were taken from the small and large intestine and corresponding mesenteric lym ... | 2003 | 14554124 |
| experimental infection of weaner sheep with s strain mycobacterium avium subsp. paratuberculosis. | we sought to determine whether infection of recently weaned 12-16-week-old merino lambs with an australian s strain m. a. paratuberculosis, at doses consistent with natural exposure, could be detected in the first few months post-inoculation. such detection would facilitate the use of weaner sheep as sentinel animals for the presence of infectious doses of m. a. paratuberculosis on pastures. in controlled pen trials, oral doses of approximately 10(7)-10(8) viable organisms were demonstrated to b ... | 2003 | 14559172 |
| is900/eric-pcr as a tool to distinguish mycobacterium avium subsp. paratuberculosis from closely related mycobacteria. | there is an increasing demand for fast and reliable methods to distinguish mycobacterium avium subsp. paratuberculosis (m. paratuberculosis) from closely related mycobacteria and also a need for rapid strain specific typing of clinical isolates for epidemiological reasons. in the present study, the potential of rep-pcr as a fingerprinting method for m. paratuberculosis was assessed and compared to conventional rflp. a pcr assay was designed and optimised to obtain reproducible fingerprints of my ... | 2003 | 14559175 |
| immune responses after oral inoculation of weanling bison or beef calves with a bison or cattle isolate of mycobacterium avium subsp. paratuberculosis. | paratuberculosis is endemic in domestic and wild ruminants worldwide. we designed the following study to compare host immune responses and pathologic changes in beef calves and bison calves after challenge with either a cattle or bison (bison bison) strain of mycobacterium avium subsp. paratuberculosis. in the first part of the study, six bison and six beef calves were orally inoculated with a cattle isolate of m. avium subsp. paratuberculosis over a 2 wk period. in the second part, an additiona ... | 2003 | 14567215 |
| enhanced expression of interleukin-1alpha and tumor necrosis factor receptor-associated protein 1 in ileal tissues of cattle infected with mycobacterium avium subsp. paratuberculosis. | infection with mycobacterium avium subsp. paratuberculosis is associated with high levels of morbidity, decreased production, and early culling in dairy cattle. clinical symptoms of johne's disease include persistent diarrhea, inappetence, and resultant weight loss due to chronic inflammation of the small intestine. although the presence or absence of intestinal lesions cannot be used as a definitive indicator of m. avium subsp. paratuberculosis infection, most infected cattle exhibit significan ... | 2003 | 14573670 |
| evidence for a novel gene expression program in peripheral blood mononuclear cells from mycobacterium avium subsp. paratuberculosis-infected cattle. | a bovine-specific cdna microarray system was used to compare gene expression profiles of peripheral blood mononuclear cells (pbmcs) from control uninfected (n = 4) and johne's disease-positive (n = 6) holstein cows. microarray experiments were designed so that for each animal, a direct comparison was made between pbmcs stimulated in vitro with mycobacterium avium subsp. paratuberculosis and pbmcs stimulated with phosphate-buffered saline (nil-stimulated pbmcs). as expected, m. avium subsp. parat ... | 2003 | 14573671 |
| variation in resistance of mycobacterium paratuberculosis to acid environments as a function of culture medium. | acid resistance of mycobacterium paratuberculosis was examined as a function of growth conditions (i.e., in vitro growth medium and ph). m. paratuberculosis was cultured in either fatty acid-containing medium (7h9-oadc) or glycerol-containing medium (wr-gd or 7h9-gd) at two culture phs (phs 6.0 and 6.8). organisms produced in these six medium and ph conditions were then tested for resistance to acetate buffer at phs 3, 4, 5, and 6 at 20 degrees c. a radiometric culture method (bactec) was used t ... | 2003 | 14602647 |
| study of animal-borne infections in the mucosas of patients with inflammatory bowel disease and population-based controls. | crohn's disease may be triggered by an infection, and it is plausible to consider that such an infection may be animal borne and ingested with our food. there has been considerable interest in the past in determining whether mycobacterium avium subsp. paratuberculosis (m. avium) might be the etiologic agent in crohn's disease since it causes a disease in cattle that is similar to crohn's disease in humans. we aimed to determine if there was an association between crohn's disease and infection wi ... | 2003 | 14605128 |
| characterization of genetic differences between mycobacterium avium subsp. paratuberculosis type i and type ii isolates. | a combination of representational difference analysis and comparative dna sequencing revealed that four type i (sheep) isolates of mycobacterium avium subsp. paratuberculosis were differentiated from nine type ii (bovine) isolates by the presence of an 11-bp insertion in a novel m. avium subsp. paratuberculosis-specific region of genomic dna. further, our studies show that m. avium subsp. paratuberculosis type i isolates contain three type-specific loci that are missing in m. avium subsp. paratu ... | 2003 | 14605167 |
| evaluation of an absorbed elisa and an agar-gel immuno-diffusion test for ovine paratuberculosis in sheep in australia. | the sensitivities and specificities of an absorbed enzyme-linked immunosorbent assay (elisa) and an agar-gel immuno-diffusion (agid) test for the detection of johne's disease in sheep were estimated using data from six known infected and 12 assumed uninfected sheep flocks. sensitivities were estimated for all histologically positive sheep, as well as by histological lesion score, lesion type (paucibacillary or multibacillary) and sheep body-condition score, with elisa sensitivities estimated at ... | 2003 | 14623409 |
| b-cell epitopes in the immunodominant p34 antigen of mycobacterium avium ssp. paratuberculosis recognized by antibodies from infected cattle. | mycobacterium avium ssp. paratuberculosis (m. paratuberculosis) causes johne's disease, a chronic and fatal enteritis in ruminants. in the last stage of the disease, antibody titres rise and levels of interferon-gamma decrease, suggesting that the host-immune response is switching from a t helper 1 (th1) to a th2 profile. in infected cattle, the membrane protein p34 elicits the predominant humoral response against m. paratuberculosis. to map the b-cell epitopes of this antigen, affinity-purified ... | 2003 | 14629623 |
| herd management practices associated with paratuberculosis seroprevalence in dutch dairy herds. | we describe the paratuberculosis management practices applied in dairy herds in the netherlands. the findings from paratuberculosis seronegative and seropositive herds were compared to discover possible risk factors. in total, 370 randomly selected herds with > or =20 dairy cows were surveyed. a questionnaire was used to collect data on current and previous paratuberculosis management practices. all cattle aged > or =3 years were serologically tested for paratuberculosis using an enzyme-linked i ... | 2003 | 14633206 |
| specificity of two tests for the early diagnosis of bovine paratuberculosis based on cell-mediated immunity: the johnin skin test and the gamma interferon assay. | paratuberculosis in cattle is a chronic debilitating infectious disease caused by mycobacterium paratuberculosis. control of paratuberculosis is based on tests that principally detect advanced stages of infections: faecal culture and serology. tests measuring cell-mediated immunity (cmi) could improve control of paratuberculosis if able to diagnose mycobacterial infections earlier, before animals become infectious. a drawback of cmi tests for paratuberculosis has been a reported low specificity. ... | 2003 | 14637040 |
| mycobacterium avium subsp. paratuberculosis infection causes suppression of rantes, monocyte chemoattractant protein 1, and tumor necrosis factor alpha expression in peripheral blood of experimentally infected cattle. | blood from cattle with subclinical mycobacterium avium subsp. paratuberculosis infection was stimulated with m. avium subsp. paratuberculosis antigens, and expression of interleukin-1beta (il-1beta), tumor necrosis factor alpha (tnf-alpha), rantes, monocyte chemoattractant protein 1 (mcp-1), and il-8 was measured. expression of tnf-alpha, rantes, and mcp-1 was lower in infected than in uninfected cattle. the reduced response may weaken protective immunity and perpetuate infection. | 2003 | 14638822 |
| iodixanol development of a laboratory-scale technique to monitor the persistence of mycobacterium avium subsp. paratuberculosis in cheddar cheese. | mycobacterium avium subsp. paratuberculosis (map) is a potential human pathogen known to be present in raw milk from infected dairy herds. current pasteurisation regimes do not totally inactivate map resulting in the possibility of viable cells being present in pasteurised milk used for cheddar cheese production. a laboratory-based method, ensuring strict safety precautions, was developed to manufacture 800-g cheddar blocks, experimentally contaminated (postpasteurisation) with two different str ... | 2003 | 14665738 |
| effects of supplemental energy on metabolic and immune measurements in periparturient dairy cows with johne's disease. | the present study was designed to evaluate whether feeding supplemental energy would improve the metabolic profile and alleviate some of the immunosuppression typically noted during the periparturient period in dairy cows with johne's disease. twelve dairy cows naturally infected with mycobacterium paratuberculosis were fitted with rumen cannulas in late gestation and assigned to treatment groups: control, n = 6; or stuffed, n = 6. cows in the control group were allowed to consume feed ad libitu ... | 2003 | 14672182 |
| comparison of contamination and growth of mycobacterium avium subsp. paratuberculosis on two different media. | the purpose of the study was to compare the growth of mycobacterium avium subsp. paratuberculosis (map) and the degree of contamination on herrold's egg yolk medium (heym) and modified löwenstein-jensen medium (lj). | 2004 | 14678168 |
| evaluation of two recovery methods for detection of mycobacterium avium subsp. paratuberculosis by pcr: direct-dilution--centrifugation and c(18)-carboxypropylbetaine processing. | a duplex polymerase chain reaction (pcr)-hybridization assay based on mycobacterium avium subsp. paratuberculosis (map)-specific is900 integration sites was used to evaluate two mycobacterial recovery methods from bovine feces: a direct-dilution-centrifugation method and a c(18)-carboxypropylbetaine (cb-18)-based method. all map pcr results were confirmed for absence of inhibitors using a novel pcr system based on the rpob gene of plant chloroplasts as an internal control. the detection limits o ... | 2003 | 14680691 |
| rapid and transient activation of gene expression in peripheral blood mononuclear cells from johne's disease positive cows exposed to mycobacterium paratuberculosis in vitro. | mycobacterium avium subspecies paratuberculosis (m. paratuberculosis) is the causative agent of johne's disease in ruminants. m. paratuberculosis is a slow-growing intracellular bacterium and infections with m. paratuberculosis can persist in a subclinical state for several years. an early and appropriate t cell-mediated cytotoxic response (th1-like) to m. paratuberculosis infection is often replaced with an antibody or th 2-like response as infected animals move toward a progressively more clin ... | 2004 | 14687562 |
| expression and immunogenicity of proteins encoded by sequences specific to mycobacterium avium subsp. paratuberculosis. | the development of immunoassays specific for the diagnosis of johne's disease in cattle requires antigens specific to mycobacterium avium subsp. paratuberculosis. however, because of genetic similarity to other mycobacteria comprising the m. avium complex, no such antigens have been found. through a comparative genomics approach, 21 potential coding sequences of m. avium subsp. paratuberculosis that are not represented in any other mycobacterial species tested (n = 9) were previously identified ... | 2004 | 14715739 |
| [mycobacterium avium subspecies paratuberculosis]. | 2003 | 14716909 | |
| association between soil type and paratuberculosis in cattle herds. | to determine the association between soil type and paratuberculosis in cattle. | 2004 | 14719695 |
| immunoperoxidase studies of cell mediated immune effector cell populations in early mycobacterium avium subsp. paratuberculosis infection in sheep. | immunoperoxidase (ipx) labelling for cd4, cd8, tcr-gammadelta, wc1, cd1b, ifn-gamma, cd45r, cd56 and lysozyme was used to investigate changes in cell mediated immune effector cell populations in the intestinal peyer's patches (pp) and mesenteric lymph nodes of lambs, 2 and 4 months after experimental infection with low doses of sheep strain mycobacterium avium subsp. paratuberculosis (m. a. paratuberculosis). the organism was cultured from the tissues of each infected lamb, but histological lesi ... | 2004 | 14741134 |
| evaluation of an automated system for non-radiometric detection of mycobacterium avium paratuberculosis in bovine feces. | cultivation of mycobacterium avium subsp. paratuberculosis (m. paratuberculosis) from feces remains the most reliable method to detect infected animals. the purpose of this study was to evaluate a broth-based automated system used for cultivation of mycobacteria such as m. tuberculosis from human hosts, for the detection of m. paratuberculosis in bovine feces. bovine feces was spiked with tenfold serial dilutions of m. paratuberculosis (5x10(5) to 5x10(-1) organisms), then processed with a doubl ... | 2004 | 14744455 |
| evaluation of four dna extraction methods for the detection of mycobacterium avium subsp. paratuberculosis by polymerase chain reaction. | polymerase chain reaction (pcr) has been widely used due to its high specificity, sensitivity, and rapid turn-around time. however, inhibitory factors may be co-extracted with the target nucleic acid that will hinder the performance of pcr. in this study, dna extraction methods for mycobacterium avium subsp. paratuberculosis were evaluated including rapid lysis, organic extraction, silica-based and magnetic particle-based (magazorb) technologies on bacterial cells, and spiked bovine feces. effic ... | 2004 | 14761720 |
| [action of pas on mycobacteria; differentiation of tubercle bacilli from the paratuberculous]. | 1950 | 14779435 | |
| preliminary studies on the effect of streptomycin and other agents on mycobacterium paratuberculosis. | 1950 | 14783259 | |
| [differentiation of tubercle bacillus and paratuberculosis bacillus by thiosemicarbazone of para-acetylaminobenzaldehyde]. | 1950 | 14801898 | |
| observations on the isolation of mycobacterium johnei in primary culture. | 1950 | 14804242 | |
| [effect of koch's and part-tuberculous bacilli on erythrocyte color change]. | 1950 | 14822283 | |
| a johne's disease survey. | 1951 | 14836341 | |
| some observations on johne's disease. | 1951 | 14836342 | |
| [the blood reducing effect of mycobacterium tuberculosis & paratuberculosis cultivated in dubos' media]. | 1951 | 14839884 | |
| [studies and research on mycobacteria. vi. effect of pas on mycobacteria; differentiation of tubercle and paratuberculosis bacilli]. | 1950 | 14844765 | |
| [diagnosis of pulmonary tuberculosis and paratuberculosis bacilli]. | 1951 | 14850022 | |
| varieties of mycobacterium johnei isolated from sheep. | 1951 | 14851174 | |
| a comparative study of the intradermal johnin test on cattle artificially and naturally sensitized with mycobacterium paratuberculosis. | 1951 | 14878120 | |
| the growth of mycobacterium tuberculosis and mycobacterium johnei in a modification of dubos medium. | 1952 | 14927872 | |
| mycobacterium avium sub. paratuberculosis in tissue samples of crohn's disease patients. | crohn's disease is a non-specific chronic transmural inflammatory disease. the disease was associated with a frameshit mutation in the nod2 gene. nevertheless, other researchers associated the presence of m. paratuberculosis within the intestinal tissues of patients with the disease. an adapted "in situ hybridization" technique was used to detect is900 m. paratuberculosis dna in paraffin embedded tissue from crohns tissue disease samples. we were able to identify m. paratuberculosis dna in aroun ... | 2004 | 14964409 |
| comparative evaluation of pcr assays for the robust molecular detection of mycobacterium avium subsp. paratuberculosis. | mycobacterium avium subsp. paratuberculosis (map) can cause a very serious, often-fatal disease, namely paratuberculosis, in several animal species, especially ruminants. recently, it has also been implicated in the pathogenesis of infectious bowel disease of man. the aim of this study was to develop a molecular method for the routine detection and identification of map, from tissue samples of animal origin. the proposed assay would have to combine optimum performance and cost, with high reprodu ... | 2004 | 14967222 |
| a simple, rapid, and effective method for the extraction of mycobacterium paratuberculosis dna from fecal samples for polymerase chain reaction. | diagnosis of paratuberculosis (johne's disease) is stymied by the lack of 1 diagnostic tool that can be used to detect both subclinically and clinically infected animals. at present, fecal culture remains the single diagnostic test that can detect infection in both disease states provided the animals actively shed mycobacterium paratuberculosis in their feces. yet, fecal culture has a disadvantage associated with the protracted incubation period of 8-16 weeks before results are available. detect ... | 2004 | 14974843 |
| effects of prevalence and testing by enzyme-linked immunosorbent assay and fecal culture on the risk of introduction of mycobacterium avium subsp. paratuberculosis-infected cows into dairy herds. | a stochastic simulation model was developed to assess the risk of introduction of mycobacterium avium subsp. paratuberculosis infection into a dairy herd through purchase of female replacement cattle. the effects of infection prevalence in the source herd(s), number of females purchased, and testing by enzyme-linked immunosorbent assay (elisa) alone or elisa and fecal culture as risk mitigation strategies were evaluated. decisions about negative test results were made on a lot and individual bas ... | 2004 | 14974844 |
| a 38-kilobase pathogenicity island specific for mycobacterium avium subsp. paratuberculosis encodes cell surface proteins expressed in the host. | we have used representational difference analysis to identify a novel mycobacterium avium subsp. paratuberculosis-specific abc transporter operon (mpt), which comprises six open reading frames designated mpta to -f and is immediately preceded by two putative fur boxes. functional genomics revealed that the mpt operon is flanked on one end by a fep cluster encoding proteins involved in the uptake of fe(3+) and on the other end by a sid cluster encoding non-ribosome-dependent heterocyclic sideroph ... | 2004 | 14977927 |
| cytokine gene expression in peripheral blood mononuclear cells and tissues of cattle infected with mycobacterium avium subsp. paratuberculosis: evidence for an inherent proinflammatory gene expression pattern. | in cattle and other ruminants, infection with the intracellular pathogen mycobacterium avium subsp. paratuberculosis results in a granulomatous enteritis (johne's disease) that is often fatal. the key features of host immunity to m. avium subsp. paratuberculosis infection include an appropriate early proinflammatory and cytotoxic response (th1-like) that eventually gives way to a predominant antibody-based response (th2-like). clinical disease symptoms often appear subsequent to waning of the th ... | 2004 | 14977946 |
| crohn's disease, mycobacteria, and nod2. | 2004 | 14998497 | |
| rapid and sensitive detection of mycobacterium avium subsp. paratuberculosis in bovine milk and feces by a combination of immunomagnetic bead separation-conventional pcr and real-time pcr. | immunomagnetic bead separation coupled with bead beating and real-time pcr was found to be a very effective procedure for the isolation, separation, and detection of mycobacterium avium subsp. paratuberculosis from milk and/or fecal samples from cattle and american bison. samples were spiked with m. avium subsp. paratuberculosis organisms, which bound to immunomagnetic beads and were subsequently lysed by bead beating; then protein and cellular contaminants were removed by phenol-chloroform-isop ... | 2004 | 15004056 |
| population-based case control study of seroprevalence of mycobacterium paratuberculosis in patients with crohn's disease and ulcerative colitis. | there is renewed enthusiasm for exploring the possibility that mycobacterium paratuberculosis may be causative in crohn's disease (cd). we aimed to determine whether cd subjects are more likely to be m. paratuberculosis seropositive than controls. using our population-based university of manitoba inflammatory bowel disease research registry, we recruited cd and ulcerative colitis (uc) subjects between 18 and 50 years of age for a study involving detailed questionnaires and venipuncture. we acces ... | 2004 | 15004064 |
| possible association of groes and antigen 85 proteins with heat resistance of mycobacterium paratuberculosis. | conflicting reports on the heat resistance of mycobacterium paratuberculosis prompted an examination of the effect of culture medium on this property of the organism. m. paratuberculosis was cultured in three types of media (fatty acid-containing medium 7h9-oadc (oleic acid-albumin-dextrose-catalase supplement) and glycerol-containing media wr-gd and 7h9-gd [glycerol-dextrose supplement]) at ph 6.0. m. paratuberculosis grown under these three culture conditions was then tested for heat resistanc ... | 2004 | 15006794 |
| a novel is element, ismpa1, in mycobacterium avium subsp. paratuberculosis. | a novel insertion element belonging to the is110 family was identified in mycobacterium avium subsp. paratuberculosis. the is element, ismpa1, is 1500 bp and has one orf encoding a putative transposase. three copies of ismpa1 were identified in the m. avium subsp. paratuberculosis genome. the element had inserted into the 3' end of the highly conserved mycobacterial genes prrb and a homologue of m. tuberculosis rv1593c, and between a putative cytochrome p450 oxygenase and a putative hydrolase. t ... | 2004 | 15036538 |
| effects of gamma interferon, interleukin-10, and transforming growth factor beta on the survival of mycobacterium avium subsp. paratuberculosis in monocyte-derived macrophages from naturally infected cattle. | gamma interferon (ifn-gamma) plays a significant role in the control of mycobacterial infections, including mycobacterium avium subsp. paratuberculosis. however, the contribution of other immunoregulatory cytokines, such as interleukin-10 (il-10) and transforming growth factor beta (tgf-beta), in johne's disease has not been investigated as yet. in this study, we examined the effects of in vivo and in vitro infection with m. avium subsp. paratuberculosis on the production of ifn-gamma, il-10, an ... | 2004 | 15039317 |
| neutralization of interleukin-10 significantly enhances gamma interferon expression in peripheral blood by stimulation with johnin purified protein derivative and by infection with mycobacterium avium subsp. paratuberculosis in experimentally infected cattle with paratuberculosis. | monoclonal antibody neutralization of interleukin-10 (il-10) increased johnin purified protein derivative-induced whole-blood gamma interferon (ifn-gamma) secretion 23-fold and also increased ifn-gamma secretion ninefold following in vitro mycobacterium avium subsp. paratuberculosis infection of peripheral blood mononuclear cells. these results demonstrate the suppressive effect of il-10 on immune responses to m. avium subsp. paratuberculosis infection in cattle. | 2004 | 15039374 |
| [detection of mycobacterium avium subsp. paratuberculosis from the testicles of a clinically infected breeding animal]. | during a post mortem of a six year old simmental bull with severe paratuberculosis infection the testicles were further examined by pathological, histological and microbiological methods. no gross or histological lesions could be observed. single acid fast organisms were detected in smears taken from sterile testicle tissue. tissue material was additionally cultured in mycostatin culture media and after 8 weeks of incubation acid fast colonies were demonstrated. polymerase chain reaction with dn ... | 2004 | 15046460 |