Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| microbiological and chemical characteristics of tarubá, an indigenous beverage produced from solid cassava fermentation. | the aim of this work was to identify and characterize the microbiota present during fermentation and in the final beverage, tarubá, by culture-dependent and -independent methods. in addition, target chemical compounds (carbohydrates, organic acids, and ethanol) were evaluated. lactic acid bacteria (lab) and mesophilic bacteria were the predominant microorganisms. among them, lactobacillus plantarum, lactobacillus brevis, leuconostoc mesenteroides, and bacillus subtilis were frequently isolated a ... | 2015 | 25846929 |
| single bacteria movement tracking by online microscopy--a proof of concept study. | in this technical report we demonstrate a low-cost online unit allowing movement tracking of flagellated bacteria on a single-cell level during fermentation processes. the system's ability to distinguish different metabolic states (viability) of bacteria by movement velocity was investigated. a flow-through cuvette with automatically adjustable layer thickness was developed. the cuvette can be used with most commercially available laboratory microscopes equipped with 40× amplification and a digi ... | 2015 | 25849813 |
| in-vitro antibacterial, antifungal, antioxidant and functional properties of bacillus amyloliquefaciens. | food born pathogenic bacteria and filamentous fungi are able to grow on most foods, including natural foods, processed foods, and fermented foods and create considerable economic loss. the aim of this study was to determine the antibacterial, antifungal, antioxidant and functional properties of bacillus amyloliquefaciens recovered from silage. | 2015 | 25858278 |
| application of a new xylanase activity from bacillus amyloliquefaciens xr44a in brewer's spent grain saccharification. | cellulases and xylanases are the key enzymes involved in the conversion of lignocelluloses into fermentable sugars. western ghat region (india) has been recognized as an active hot spot for the isolation of new microorganisms. the aim of this work was to isolate new microorganisms producing cellulases and xylanases to be applied in brewer's spent grain saccharification. | 2015 | 25866429 |
| draft genome sequence of bacillus amyloliquefaciens ap183 with antibacterial activity against methicillin-resistant staphylococcus aureus. | bacillus amyloliquefaciens ap183 expresses secondary metabolites that inhibit the growth of methicillin-resistant staphylococcus aureus (mrsa). here, we present a ~3.99-mbp draft genome sequence of ap183 with the aims of providing insights into the genomic basis of its antibacterial mechanisms and exploring its potential use in preventing mrsa skin colonization. | 2015 | 25883273 |
| improving the quality of laminaria japonica-based diet for apostichopus japonicus through degradation of its algin content with bacillus amyloliquefaciens wb1. | laminaria japonica feedstuff is used as a substitute for sargassum thunbergii in the small-scale culturing of apostichopus japonicus (sea cucumber) because of its abundant sources and low price in china. however, the difficulty associated with the degradation of algin by a. japonicus and, hence, its utilization have limited the practical value of l. japonica feedstuff in sea cucumber farming. in this study, a. japonicus individuals were fed with l. japonica feedstuff pretreated, via fermentation ... | 2015 | 25895094 |
| chitin extraction from crab shells by bacillus bacteria. biological activities of fermented crab supernatants. | crab shells waste were fermented using six protease-producing bacillus species (bacillus subtilis a26, bacillus mojavensis a21, bacillus pumilus a1, bacillus amyloliquefaciens an6, bacillus licheniformis nh1 and bacillus cereus bg1) for the production of chitin and fermented-crab supernatants (fcss). in medium containing only crab shells, the highest demineralization dm was obtained with b. licheniformis nh1 (83±0.5%) and b. pumilus a1 (80±0.6%), while the highest deproteinization (dp) was achie ... | 2015 | 25910648 |
| a plasmid-born rap-phr system regulates surfactin production, sporulation and genetic competence in the heterologous host, bacillus subtilis okb105. | according to the change of environment, soil-dwelling bacillus species differentiate into distinct subpopulations, such as spores and competent cells. rap-phr systems have been found to be involved in this differentiation circuit by interacting with major regulatory proteins, such as spo0a, coma, and degu. in this study, we report that the plasmid-born rapq-phrq system found in bacillus amyloliquefaciens b3 affects three regulatory pathways in the heterologous host bacillus subtilis. expression ... | 2015 | 25921807 |
| construction of a bacillus amyloliquefaciens strain for high purity levan production. | bacillus amyloliquefaciens nk-1 has the potential to produce levan and poly-gamma-glutamic acid (γ-pga) simultaneously. however, it is not possible to purify each single product from the same strain because the extraction process is identical. we deleted the pgs cluster (for γ-pga synthesis) from the nk-1 strain and constructed a γ-pga-deficient nk-δlp strain. nuclear magnetic results showed that the nk-δlp strain could produce high purity levan product. however, its levan titer was only 1.96 g ... | 2015 | 25953857 |
| nano titania aided clustering and adhesion of beneficial bacteria to plant roots to enhance crop growth and stress management. | a novel use of titania nanoparticles as agents in the nano interface interaction between a beneficial plant growth promoting bacterium (bacillus amyloliquefaciens ucmb5113) and oilseed rape plants (brassica napus) for protection against the fungal pathogen alternaria brassicae is presented. two different tio2 nanoparticle material were produced by the sol-gel approach, one using the patented captigel method and the other one applying tibaldh precursor. the particles were characterized by transmi ... | 2015 | 25970693 |
| synergistic effects of [ile⁷]surfactin homologues with bacillomycin d in suppression of gray mold disease by bacillus amyloliquefaciens biocontrol strain sd-32. | we previously reported that bacillus amyloliquefaciens biocontrol strain sd-32 produces powerful antifungal lipopeptides, c17 bacillomycin d homologues. in the course of the investigation we found that the antifungal activity of the culture supernatant of this bacterium was not ascribed exclusively to bacillomycin d. we attempted to identify metabolites other than bacillomycin d to gain insight into the mechanism for the biocontrol by this bacterium. after purifying the fractions of the culture ... | 2015 | 25976169 |
| diversity of food-borne bacillus volatile compounds and influence on fungal growth. | to evaluate the antifungal activity of the volatile organic compounds (vocs) produced by 75 different food-borne bacillus species against aspergillus niger, aspergillus flavus, aspergillus parasiticus, aspergillus clavatus, fusarium oxysporum f. sp. lactucae and moniliophthora perniciosa and to determine the vocs responsible for the inhibition. | 2015 | 25989039 |
| pectin enhances bio-control efficacy by inducing colonization and secretion of secondary metabolites by bacillus amyloliquefaciens sqy 162 in the rhizosphere of tobacco. | bacillus amyloliquefaciens is a plant-beneficial gram-positive bacterium involved in suppressing soil-borne pathogens through the secretion of secondary metabolites and high rhizosphere competence. biofilm formation is regarded as a prerequisite for high rhizosphere competence. in this work, we show that plant extracts affect the chemotaxis and biofilm formation of b. amyloliquefaciens sqy 162 (sqy 162). all carbohydrates tested induced the chemotaxis and biofilm formation of the sqy 162 strain; ... | 2015 | 25996156 |
| bacillus amyloliquefaciens supplementation alleviates immunological stress in lipopolysaccharide-challenged broilers at early age. | this study was conducted to investigate the effect of bacillus amyloliquefaciens ( ba: ) on the immune function of broilers challenged with lipopolysaccharide ( lps: ). 192 one-day-old male arbor acre broiler chickens were randomly distributed into four treatments: 1) broilers fed a basal diet; 2) broilers fed a basal diet supplemented with ba; 3) lps-challenged broilers fed a basal diet; and 4) lps-challenged broilers fed a basal diet supplemented with ba. each treatment consisted of six replic ... | 2015 | 26009750 |
| structure and absolute configuration of kongiidiazadione, a new phytotoxic 3-substituted-5-diazenylcyclopentendione produced by diaporthe kongii. | a new 3-substituted-5-diazenylcyclopentendione named kongiidiazadione was isolated from culture filtrates of diaporthe kongii, associated with stem cankers on sunflower in australia. kongiidiazadione was characterized by spectroscopic (essentially nuclear magnetic resonance [nmr] and high-resolution, electrospray ionization, mass spectrometry [hresims]) methods as (-)-5-diazenyl-3-hydroxymethyl-cyclopent-3-en-1,2-dione. the stereochemistry of the diazenyl group was determined by ir spectroscopy, ... | 2015 | 26011252 |
| cyclic lipopeptides of bacillus amyloliquefaciens subsp. plantarum colonizing the lettuce rhizosphere enhance plant defense responses toward the bottom rot pathogen rhizoctonia solani. | the commercially available inoculant bacillus amyloliquefaciens fzb42 is able to considerably reduce lettuce bottom rot caused by rhizoctonia solani. to understand the interaction between fzb42 and r. solani in the rhizosphere of lettuce, we used an axenic system with lettuce bacterized with fzb42 and inoculated with r. solani. confocal laser scanning microscopy showed that fzb42 could delay the initial establishment of r. solani on the plants. to show which secondary metabolites of fzb42 are pr ... | 2015 | 26011557 |
| possibility of biological control of primocane fruiting raspberry disease caused by fusarium sambucinum. | biological control agents are a promising alternative to chemical pesticides for plant disease suppression. the main advantage of the natural biocontrol agents, such as antagonistic bacteria compared with chemicals, includes environmental pollution prevention and a decrease of chemical residues in fruits. this study is aimed to evaluate the impact of three bacillus strains on disease of primocane fruiting raspberry canes caused by fusarium sambucinum under controlled infection load and uncontrol ... | 2015 | 26018288 |
| mutagenetic study of a novel inosine monophosphate dehydrogenase from bacillus amyloliquefaciens and its possible application in guanosine production. | in this study, the amino acid sequence of inosine monophosphate dehydrogenase (impdh) from a guanosine-overproducing strain bacillus amyloliquefaciens ta208 was found to be highly conserved comparing to its analogue in b. amyloliquefaciens fzb42, only with two substitutions of serine 166 to proline and glutamic acid 481 to lysine. to speculate on the effects of these variation sites, two reverse site-directed mutants p166s and k481e, as well as one deletion mutant impdh(δcbs), were characterised ... | 2014 | 26019494 |
| comparative genomics within the bacillus genus reveal the singularities of two robust bacillus amyloliquefaciens biocontrol strains. | bacillus amyloliquefaciens cect 8237 and cect 8238, formerly known as bacillus subtilis umaf6639 and umaf6614, respectively, contribute to plant health by facing microbial pathogens or inducing the plant's defense mechanisms. we sequenced their genomes and developed a set of ad hoc scripts that allowed us to search for the features implicated in their beneficial interaction with plants. we define a core set of genes that should ideally be found in any beneficial bacillus strain, including the pr ... | 2015 | 26035127 |
| [the taxonomic study of the strain bacillus sp. ucm b-7404--phytopathogenic fungae antagonist]. | the polyphasic taxonomic analysis of strain bacillus sp. ucm b-7404 active against phytopathogenic fungi and producing extracellular phytohormones, lytic enzymes and lipopeptide antifungal compounds has been carried out. the basic cell wall fatty acids presented by branched iso- and anteiso- c15:0 and c17:0 acids, contained 65-77% of the average pool. phylogenetic assay of 16s rrna gene nucleotide sequence, phenotypic and chemotaxonomic characteristics proved the attachment of strain bacillus sp ... | 2015 | 26036027 |
| bacillus oryzicola sp. nov., an endophytic bacterium isolated from the roots of rice with antimicrobial, plant growth promoting, and systemic resistance inducing activities in rice. | biological control of major rice diseases has been attempted in several rice-growing countries in asia during the last few decades and its application using antagonistic bacteria has proved to be somewhat successful for controlling various fungal diseases in field trials. two novel endophytic bacillus species, designated strains yc7007 and yc7010(t), with anti-microbial, plant growth-promoting, and systemic resistance-inducing activities were isolated from the roots of rice in paddy fields at ji ... | 2015 | 26060434 |
| cloning, enhanced expression and characterization of an α-amylase gene from a wild strain in b. subtilis wb800. | a bacillus strain with high productivity of α-amylase isolated from a starch farm was identified as bacillus amyloliquefaciens. the α-amylase encoding gene amy1 was cloned into pmd18-t vector and amplified in e. coli dh5α. shuttle vector pp43mnx was reconstructed to obtain vector pp43x for heterologous expression of the α-amylase in b. subtilis wb800. recombinant enzyme was sufficiently purified by precipitation, gel filtration and anion exchange with a specific activity of 5566 u/mg. the α-amyl ... | 2015 | 26092061 |
| purification and identification of two antifungal cyclic peptides produced by bacillus amyloliquefaciens l-h15. | bacillus amyloliquefaciens l-h15 with broad spectrum antifungal activity was used as a biocontrol agent to suppress fusarium oxysporum and other soil-borne fungal plant pathogens. two antifungal fractions were isolated by bioactivity-guided reversed-phase high-performance liquid chromatography. the two compounds were identified by tandem q-tof mass spectroscopy as c15 iturin a (1) and a novel cyclic peptide with a molecular weight of 852.4 da (2). both compounds showed good inhibitory activities ... | 2015 | 26123083 |
| the global transcriptional landscape of bacillus amyloliquefaciens xh7 and high-throughput screening of strong promoters based on rna-seq data. | bacillus amyloliquefaciens is an important industrial microbe for the production of many industrial enzymes and primary metabolites. although the complete genome sequence of b. amyloliquefaciens has been now published, transcript structures of b. amyloliquefaciens remain poorly defined. in this study, high-throughput rna sequencing (rna-seq) technology was applied to dissect the transcriptome of b. amyloliquefaciens strain xh7. in total, 3936 out of a total of 4204 b. amyloliquefaciens genes (93 ... | 2015 | 26133043 |
| separation, determination and antifungal activity test of the products from a new bacillus amyloliquefaciens. | a new bacillus amyloliquefaciens named zju-2011 was discovered, and the culture supernatant showed a strong inhibitory effect against candida albicans. in this study, a novel method was developed to purify the antifungal compounds in high purity. the obtained products were analysed by high performance liquid chromatography and proven to be of high purity. mass spectrometry showed that the molecular weights of the two bioactive components were 270 and 288, respectively, and their structures were ... | 2016 | 26140441 |
| bioactive peptides identified in thornback ray skin's gelatin hydrolysates by proteases from bacillus subtilis and bacillus amyloliquefaciens. | thornback ray skin gelatin has been hydrolyzed with two different proteases in order to obtain peptides with ace inhibitory and antioxidant activity. hydrolysates with protease from bacillus subtilis a26 (trgh-a26) displayed ace inhibitory activity with an ic50 value of 0.94 μg/μl whereas neutrase® hydrolysate from bacillus amyloliquefaciens (trgh-neutrase) showed an ic50 value of 2.07 μg/μl. regarding antioxidant activity, ic50 values of 1.98 and 21.2 μg/μl in trgh-a26 and trgh-neutrase, respec ... | 2015 | 26149667 |
| bacillus amyloliquefaciens subsp. plantarum gr53, a potent biocontrol agent resists rhizoctonia disease on chinese cabbage through hormonal and antioxidants regulation. | the fungus rhizoctonia solani is one of the causal agents of numerous diseases that affect crop growth and yield. the aim of this present investigation was to identify a biocontrol agent that acts against r. solani and to determine the agent's protective effect through phytohormones and antioxidant regulation in experimentally infected chinese cabbage plants. four rhizospheric soil bacterial isolates gr53, gr169, gr786, and gr320 were tested for their antagonistic activity against r. solani. amo ... | 2015 | 26160009 |
| characterization of a mannose-6-phosphate isomerase from bacillus amyloliquefaciens and its application in fructose-6-phosphate production. | the bam6pi gene encoding a mannose-6-phosphate isomerase (m6pi, ec 5.3.1.8) was cloned from bacillus amyloliquefaciens dsm7 and overexpressed in escherichia coli. the enzyme activity of bam6pi was optimal at ph and temperature of 7.5 and 70°c, respectively, with a kcat/km of 13,900 s-1 mm-1 for mannose-6-phosphate (m6p). the purified bam6pi demonstrated the highest catalytic efficiency of all characterized m6pis. although m6pis have been characterized from several other sources, bam6pi is distin ... | 2015 | 26171785 |
| characterization of an extremely salt-tolerant and thermostable phytase from bacillus amyloliquefaciens us573. | the extracellular phytase produced by the bacillus amyloliquefaciens us573 strain, isolated from geothermal soil located in southern tunisia was purified and characterized. this calcium-dependent and bile-stable enzyme (phy us573) was optimally active at ph 7.5 and 70 °c. it showed a good stability at ph ranging from 4 to 10, and especially, an exceptional thermostability as it recovered 50 and 62% of activity after heating for 10 min at 100 and 90 °c, respectively. in addition, phy us573 was fo ... | 2015 | 26188308 |
| induction of systemic resistance in panax ginseng against phytophthora cactorum by native bacillus amyloliquefaciens hk34. | korean ginseng (panax ginseng meyer) is a perennial herb prone to various root diseases, with phytophthora cactorum being considered one of the most dreaded pathogens. p. cactorum causes foliar blight and root rot. although chemical pesticides are available for disease control, attention has been shifted to viable, eco-friendly, and cost-effective biological means such as plant growth-promoting rhizobacteria (pgpr) for control of diseases. | 2015 | 26199552 |
| impact of different water activities (a w) adjusted by solutes on high pressure high temperature inactivation of bacillus amyloliquefaciens spores. | much research has been conducted to comprehend the mechanisms of high pressure (hp) inactivation of spores in aqueous systems but for food model systems these information are scarce. in these systems spores can interact with ingredients which then could possibly lead to retarded or reduced inactivation, which can cause a problem for the sterilization process. the protective mechanism of a reduced a w-value is still unclear. hp processing might prove valuable to overcome protective effects of sol ... | 2015 | 26217321 |
| bacterial respiration and growth rates affect the feeding preferences, brood size and lifespan of caenorhabditis elegans. | bacteria serve as live food and nutrients for bacterial-feeding nematodes (bfns) in soils, and influence nematodes behavior and physiology through their metabolism. five bacterial taxa (bacillus amyloliquefaciens jx1, variovorax sp. jx14, bacillus megaterium jx15, pseudomonas fluorescens y1 and escherichia coli op50) and the typical bfn caenorhabditis elegans were selected to study the effects of bacterial respiration and growth rates on the feeding preferences, brood size and lifespan of nemato ... | 2015 | 26222828 |
| ultra high pressure homogenization (uhph) inactivation of bacillus amyloliquefaciens spores in phosphate buffered saline (pbs) and milk. | ultra high pressure homogenization (uhph) opens up new areas for dynamic high pressure assisted thermal sterilization of liquids. bacillus amyloliquefaciens spores are resistant to high isostatic pressure and temperature and were suggested as potential surrogate for high pressure thermal sterilization validation. b. amyloliquefaciens spores suspended in pbs buffer (0.01 m, ph 7.0), low fat milk (1.5%, ph 6.7), and whole milk (3.5%, ph 6.7) at initial concentration of ~10(6) cfu/ml were subjected ... | 2015 | 26236296 |
| study of biological degradation of new poly(ether-urethane-urea)s containing cyclopeptide moiety and peg by bacillus amyloliquefaciens isolated from soil. | the present work for the first time investigates the effect of bacillus amyloliquefaciens, m3, on a new poly(ether-urethane-urea) (peuu). peuu was synthesized via reaction of 4,4'-methylenebis(4-phenylisocyanate) (mdi), l-leucine anhydride cyclopeptide (lacp) as a degradable monomer and polyethylene glycol with molecular weight of 1000 (peg-1000). biodegradation of the synthesized peuu as the only source for carbon and nitrogen for m3 was studied. the co-metabolism biodegradation of the polymer ... | 2015 | 26242387 |
| difficidin and bacilysin from bacillus amyloliquefaciens fzb42 have antibacterial activity against xanthomonas oryzae rice pathogens. | bacterial blight and bacterial leaf streak are serious, economically damaging, diseases of rice caused by the bacteria xanthomonas oryzae pv. oryzae and x. oryzae pv. oryzicola. bacillus amyloliquefaciens fzb42 was shown to possess biocontrol activity against these xanthomonas strains by producing the antibiotic compounds difficidin and bacilysin. analyses using fluorescence, scanning electron and transmission electron microscopy revealed difficidin and bacilysin caused changes in the cell wall ... | 2015 | 26268540 |
| complete genome sequence of bacillus amyloliquefaciens strain co1-6, a plant growth-promoting rhizobacterium of calendula officinalis. | the genome sequence of bacillus amyloliquefaciens strain co1-6, a plant growth-promoting rhizobacterium (pgpr) with broad-spectrum antagonistic activity against plant-pathogenic fungi, bacteria, and nematodes, consists of a single 3.9-mb circular chromosome. the genome reveals genes putatively responsible for its promising biocontrol and pgp properties. | 2015 | 26272562 |
| biocontrol mechanism by root-associated bacillus amyloliquefaciens fzb42 - a review. | bacillus amyloliquefaciens subsp. plantarum fzb42 is a gram-positive model bacterium for unraveling plant-microbe interactions in bacilli. in addition, fzb42 is used commercially as biofertilizer and biocontrol agent in agriculture. genome analysis of fzb42 revealed that nearly 10% of the fzb42 genome is devoted to synthesizing antimicrobial metabolites and their corresponding immunity genes. however, recent investigations in planta demonstrated that - except surfactin - the amount of such compo ... | 2015 | 26284057 |
| enhanced 2,3-butanediol production from biodiesel-derived glycerol by engineering of cofactor regeneration and manipulating carbon flux in bacillus amyloliquefaciens. | bacillus amyloliquefaciens b10-127 exhibited an excellent ability for industrial-scale microbial fermentation of 2,3-butanediol (2,3-bd) from biodiesel-derived glycerol. however, the accumulation of by-products (acetoin, acetoin, lactate and succinate) and the 2,3-bd yield remains prohibitively low for commercial production. | 2015 | 26296537 |
| complete genome sequence of bacillus amyloliquefaciens l-s60, a plant growth-promoting and antifungal bacterium. | bacillus amyloliquefaciens l-s60, a gram-positive plant-associated bacterium, which could stimulate plant growth and shows strong antifungal function, was isolated from the turfy soil in beijing, china. the genome of b. amyloliquefaciens l-s60 comprises a 3903,017bp long circular chromosome that consists of 3909 protein-coding genes and 117 rna genes. based on genomic analysis, we identified gene clusters responsible for the biosynthesis of numerous bioactive metabolites with well-established in ... | 2015 | 26297906 |
| bacillus amyloliquefaciens ag1 biosurfactant: putative receptor diversity and histopathological effects on tuta absoluta midgut. | the use of biosurfactant in pest management has received much attention for the control of plant pathogens, but few studies reported their insecticidal activity. the present study describes the insecticidal activity of biosurfactant extracted from bacillus amyloliquefaciens strain ag1. this strain produces a lipopeptide biosurfactant exhibiting an lc50 of about 180ng/cm(2) against tuta absoluta larvae. accordingly, the histopathologic effect of this biosurfactant on t. absoluta larvae showed ser ... | 2015 | 26299754 |
| organic acids from root exudates of banana help root colonization of pgpr strain bacillus amyloliquefaciens njn-6. | the successful colonization of plant growth promoting rhizobacteria (pgpr) in the rhizosphere is an initial and compulsory step in the protection of plants from soil-borne pathogens. therefore, it is necessary to evaluate the role of root exudates in the colonization of pgpr. banana root exudates were analyzed by high pressure liquid chromatography (hplc) which revealed exudates contained several organic acids (oas) including oxalic, malic and fumaric acid. the chemotactic response and biofilm f ... | 2015 | 26299781 |
| construction of a highly efficient bacillus subtilis 168 whole-cell biocatalyst and its application in the production of l-ornithine. | l-ornithine, a non-protein amino acid, is usually extracted from hydrolyzed protein as well as produced by microbial fermentation. here, we focus on a highly efficient whole-cell biocatalyst for the production of l-ornithine. the gene argi, encoding arginase, which catalyzes the hydrolysis of l-arginine to l-ornithine and urea, was cloned from bacillus amyloliquefaciens b10-127 and expressed in gras strain bacillus subtilis 168. the recombinant strain exhibited an arginase activity of 21.9 u/mg, ... | 2015 | 26314414 |
| analysis and cloning of the synthetic pathway of the phytohormone indole-3-acetic acid in the plant-beneficial bacillus amyloliquefaciens sqr9. | the plant growth-promoting rhizobacteria (pgpr) strain bacillus amyloliquefaciens sqr9, isolated from the cucumber rhizosphere, protects the host plant from pathogen invasion and promotes plant growth through efficient root colonization. the phytohormone indole-3-acetic acid (iaa) has been suggested to contribute to the plant-growth-promoting effect of bacillus strains. the possible iaa synthetic pathways in b. amyloliquefaciens sqr9 were investigated in this study, using a combination of chemic ... | 2015 | 26337367 |
| thermal properties of barley starch and its relation to starch characteristics. | amylopectin fine structure and starch gelatinization and retrogradation were studied in 10 different barley cultivars/breeding lines. clusters and building blocks were isolated from the amylopectin by α-amylase from bacillus amyloliquefaciens and their structure was characterized. gelatinization was studied at a starch:water ratio of 1:3, and retrogradation was studied on gelatinized starch at starch:water ratio of 1:2, by differential scanning calorimetry. three barley cultivars/breeding lines ... | 2015 | 26342462 |
| bacillus amyloliquefaciens levansucrase-catalyzed the synthesis of fructooligosaccharides, oligolevan and levan in maple syrup-based reaction systems. | maple syrups with selected degree brix (°bx) (15, 30, 60) were investigated as reaction systems for levansucrase from bacillus amyloliquefaciens. the enzymatic conversion of sucrose present in the maple syrup and the production of the transfructosylation products were assessed over a time course of 48h. at 30°c, the use of maple syrup 30°bx led to the highest levansucrase activity (427.53μmol/mg protein/min), while maple syrup 66°bx led to the highest converted sucrose concentration (1.53m). in ... | 2015 | 26344273 |
| whole transcriptomic analysis of the plant-beneficial rhizobacterium bacillus amyloliquefaciens sqr9 during enhanced biofilm formation regulated by maize root exudates. | bacillus amyloliquefaciens sqr9 is a plant growth-promoting rhizobacteria (pgpr) with outstanding abilities to enhance plant growth and to control soil-borne diseases. root exudates is known to play important roles in plant-microbe interactions. to explore the rhizosphere interactions and plant-beneficial characteristics of sqr9, the complete genome sequence as well as the transcriptome in response to maize root exudates under biofilm-forming conditions were elucidated. | 2015 | 26346121 |
| recruiting a new strategy to improve levan production in bacillus amyloliquefaciens. | microbial levan is an important biopolymer with considerable potential in food and medical applications. bacillus amyloliquefaciens nk-δlp strain can produce high-purity, low-molecular-weight levan, but production is relatively low. to enhance the production of levan, six extracellular protease genes (bpr, epr, mpr, vpr, npre and apre), together with the tasa gene (encoding the major biofilm matrix protein tasa) and the pgsbca cluster (responsible for poly-γ-glutamic acid (γ-pga) synthesis), wer ... | 2015 | 26347185 |
| deciphering the conserved genetic loci implicated in plant disease control through comparative genomics of bacillus amyloliquefaciens subsp. plantarum. | to understand the growth-promoting and disease-inhibiting activities of plant growth-promoting rhizobacteria (pgpr) strains, the genomes of 12 bacillus subtilis group strains with pgpr activity were sequenced and analyzed. these b. subtilis strains exhibited high genomic diversity, whereas the genomes of b. amyloliquefaciens strains (a member of the b. subtilis group) are highly conserved. a pairwise blastp matrix revealed that gene family similarity among bacillus genomes ranges from 32 to 90%, ... | 2015 | 26347755 |
| bacillus amyloliquefaciens tsbso 3.8, a biosurfactant-producing strain with biotechnological potential for microbial enhanced oil recovery. | a screening for biosurfactant-producing bacteria was conducted with 217 strains that were isolated from environmental samples contaminated with crude oil and/or petroleum derivatives. although 19 promising biosurfactant producers were detected, strain tsbso 3.8, which was identified by molecular methods as bacillus amyloliquefaciens, drew attention for its production of a high-activity compound that presented an emulsification activity of 63% and considerably decreased surface (28.5 mn/m) and in ... | 2015 | 26350801 |
| synergistic effect of pseudomonas putida and bacillus amyloliquefaciens ameliorates drought stress in chickpea (cicer arietinum l.). | two plant growth promoting rhizobacteria (pgpr) pseudomonas putida nbrira and bacillus amyloliquefaciens nbrisn13 with ability to tolerate abiotic stress along with multiple pgp traits like acc deaminase activity, minerals solubilisation, hormones production, biofilm formation, siderophore activity were evaluated for their synergistic effect to ameliorate drought stress in chickpea. earlier we have reported both the strains individually for their pgp attributes and stress amelioration in host pl ... | 2015 | 26362119 |
| activation of antibiotic production in bacillus spp. by cumulative drug resistance mutations. | bacillus subtilis strains produce a wide range of antibiotics, including ribosomal and nonribosomal peptide antibiotics, as well as bacilysocin and neotrehalosadiamine. mutations in b. subtilis strain 168 that conferred resistance to drugs such as streptomycin and rifampin resulted in overproduction of the dipeptide antibiotic bacilysin. cumulative drug resistance mutations, such as mutations in the mtha and rpsl genes, which confer low- and high-level resistance, respectively, to streptomycin, ... | 2015 | 26369962 |
| dna-protection and antioxidant properties of fermentates from bacillus amyloliquefaciens b-1895 and bacillus subtilis katmira1933. | dna protective and antioxidant activity of bacillus amyloliquefaciens b-1895 and bacillus subtilis katmira1933 were evaluated by escherichia coli-based lux biosensors. two biosensor strains of e. coli, mg1655 (pcold-lux) and mg1655 (psoxs-lux), which react on dna damage and superoxide-anion radical activity, were used. sos-response and sox-response were stimulated by addition of dioxidine (2,3-quinoxalinedimethanol,1,4-dioxide) and paraquat (n,n'-dimethyl-4,4'-bipyridinium dichloride) respective ... | 2015 | 26370336 |
| purification and characterization of detergent stable alkaline protease from bacillus amyloliquefaciens sp1 isolated from apple rhizosphere. | a thermostable extracellular alkaline protease producing bacillus amyloliquefaciens sp1 was isolated from apple rhizosphere having multifarious plant growth promoting activities. strain sp1 was purified to 6.48-fold using four-step purification protocol and characterized in detail for its robustness and ecofriendly application in leather and detergent industries. structural analysis revealed that the protease was monomeric and had a molecular weight of 43 kda. it exhibited optimum activity at 60 ... | 2016 | 26375163 |
| biocontrol agent bacillus amyloliquefaciens lj02 induces systemic resistance against cucurbits powdery mildew. | powdery mildew is a fungal disease found in a wide range of plants and can significantly reduce crop yields. bacterial strain lj02 is a biocontrol agent (bca) isolated from a greenhouse in tianjin, china. in combination of morphological, physiological, biochemical and phylogenetic analyses, strain lj02 was classified as a new member of bacillus amyloliquefaciens. greenhouse trials showed that lj02 fermentation broth (lj02fb) can effectively diminish the occurrence of cucurbits powdery mildew. wh ... | 2015 | 26379654 |
| role of uv photolysis in accelerating the biodegradation of 2,4,6-tcp. | 2,4,6-tcp, a kind of chlorinated aromatic and aliphatic compound, is difficult to be biodegraded by ordinary microorganisms. uv photolysis and biodegradation of 2,4,6-tcp by bacillus amyloliquefaciens intimate coupling is a potential means to accelerate its biotransformation. the initial steps of 2,4,6-tcp biodegradation involve mono-oxygenation reactions that have molecular oxygen and an intracellular electron carrier as cosubstrates. it was demonstrated that b. amyloliquefaciens has the 2,4,6- ... | 2015 | 26385845 |
| accelerating sample preparation through enzyme-assisted microfiltration of salmonella in chicken extract. | microfiltration of chicken extracts has the potential to significantly decrease the time required to detect salmonella, as long as the extract can be efficiently filtered and the pathogenic microorganisms kept in a viable state during this process. we present conditions that enable microfiltration by adding endopeptidase from bacillus amyloliquefaciens to chicken extracts or chicken rinse, prior to microfiltration with fluid flow on both retentate and permeate sides of 0.2 μm cutoff polysulfone ... | 2016 | 26400739 |
| improved poly-γ-glutamic acid production in bacillus amyloliquefaciens by modular pathway engineering. | a bacillus amyloliquefaciens strain with enhanced γ-pga production was constructed by metabolically engineering its γ-pga synthesis-related metabolic networks: by-products synthesis, γ-pga degradation, glutamate precursor synthesis, γ-pga synthesis and autoinducer synthesis. the genes involved in by-products synthesis were firstly deleted from the starting nk-1 strain. the obtained nk-e7 strain with deletions of the epsa-o (responsible for extracellular polysaccharide synthesis), sac (responsibl ... | 2015 | 26410449 |
| genome sequence of antibiotic-producing bacillus amyloliquefaciens strain kctc 13012. | we report the 4.0-mb draft genome sequence of bacillus amyloliquefaciens (syn. bacillus velezensis) kctc 13012, which exhibits a broad spectrum of antagonistic activity against bacteria and fungi and promotes plant growth as well. the genome contains an array of biosynthetic gene clusters for secondary metabolites that are comparable to those in bacillus amyloliquefaciens subsp. plantarum fzb42(t). | 2015 | 26430040 |
| comparison of recombinant α-hemoglobin from crocodylus siamensis expressed in different cloning vectors and their biological properties. | hemoglobin (hb) is an important component in red blood cells of the vertebrate. it is a major respiratory protein with oxygen or carbon dioxide transport function. hb has been reported to contain bioactive peptides which have antibacterial and antioxidant activities. in this study, the alpha-chain hemoglobin(αhb) gene of crocodylus siamensis was cloned into the three different expression vectors and expressed in escherichia coli bl21 (de3). the recombinant αhb proteins from all constructs could ... | 2016 | 26455814 |
| application of byproducts from food processing for production of 2,3-butanediol using bacillus amyloliquefaciens tul 308. | a nonpathogenic bacterial strain bacillus amyloliquefaciens tul 308 synthesized minor 2,3-butanediol (2,3-bd) amounts from glucose, fructose, sucrose, and glycerol, and efficiently produced the diol from molasses and hydrolysates of food processing residues. batch fermentations yielded 16.53, 10.72, and 5 g/l 2,3-bd from enzymatic hydrolysates of apple pomace, dried sugar beet pulp, and potato pulp (at initial concentrations equivalent to 45, 20, and 30 g/l glucose, respectively), and 25.3 g/l 2 ... | 2016 | 26460787 |
| antagonistic activities of novel peptides from bacillus amyloliquefaciens pt14 against fusarium solani and fusarium oxysporum. | bacillus species have recently drawn attention due to their potential use in the biological control of fungal diseases. this paper reports on the antifungal activity of novel peptides isolated from bacillus amyloliquefaciens pt14. reverse-phase high-performance liquid chromatography revealed that b. amyloliquefaciens pt14 produces five peptides (pt14-1, -2, -3, -4a, and -4b) that exhibit antifungal activity but are inactive against bacterial strains. in particular, pt14-3 and pt14-4a showed broa ... | 2015 | 26496638 |
| evaluation of the endophytic nature of bacillus amyloliquefaciens strain gyl4 and its efficacy in the control of anthracnose. | endophytic bacteria are viewed as a potential new source of biofungicides because they have beneficial characteristics as control agents for plant disease. this study was performed to examine the endophytic feature and disease control efficacy of bacillus amyloliquefaciens strain gyl4 and to identify the antifungal compounds produced by this strain. | 2016 | 26518268 |
| microbial inoculants for the biocontrol of fusarium spp. in durum wheat. | fusarium head blight (fhb) is a severe disease caused by different fusarium species, which affects a wide range of cereal crops, including wheat. it determines from 10 to 30% of yield loss in europe. chemical fungicides are mainly used to reduce the incidence of fhb, but low environmental impact solutions are looked forward. applications of soil/rhizobacteria as biocontrol agents against fhb in wheat are described in literature, whereas the potential use of lactobacilli in agriculture has scarce ... | 2015 | 26518441 |
| ex vivo application of secreted metabolites produced by soil-inhabiting bacillus spp. efficiently controls foliar diseases caused by alternaria spp. | bacterial biological control agents (bcas) are largely used as live products to control plant pathogens. however, due to variable environmental and ecological factors, live bcas usually fail to produce desirable results against foliar pathogens. in this study, we investigated the potential of cell-free culture filtrates of 12 different bacterial bcas isolated from flower beds for controlling foliar diseases caused by alternaria spp. in vitro studies showed that culture filtrates from two isolate ... | 2015 | 26519395 |
| inhibition of bacillus cereus growth and toxin production by bacillus amyloliquefaciens rd7-7 in fermented soybean products. | bacillus cereus is a gram-positive, rod-shaped, spore-forming bacterium that has been isolated from contaminated fermented soybean food products and from the environment. b. cereus produces diarrheal and emetic toxins and has caused many outbreaks of foodborne diseases. in this study, we investigated whether b. amyloliquefaciens rd7-7, isolated from rice doenjang (korean fermented soybean paste), a traditional korean fermented soybean food, shows antimicrobial activity against b. cereus and regu ... | 2016 | 26528531 |
| kinetic properties and stability of glucose dehydrogenase from bacillus amyloliquefaciens sb5 and its potential for cofactor regeneration. | glucose dehydrogenases (gludh) from bacillus species offer several advantages over other nad(p)h regeneration systems including high stability, inexpensive substrate, thermodynamically favorable reaction and flexibility to regenerate both nadh and nadph. in this research, characteristics of gludh from bacillus amyloliquefaciens sb5 (gludh-ba) was reported for the first time. despite a highly similar amino acid sequence when comparing with gludh from bacillus subtilis (gludh-bs), gludh-ba exhibit ... | 2015 | 26538191 |
| drna-seq reveals genomewide tsss and noncoding rnas of plant beneficial rhizobacterium bacillus amyloliquefaciens fzb42. | bacillus amyloliquefaciens subsp. plantarum fzb42 is a representative of gram-positive plant-growth-promoting rhizobacteria (pgpr) that inhabit plant root environments. in order to better understand the molecular mechanisms of bacteria-plant symbiosis, we have systematically analyzed the primary transcriptome of strain fzb42 grown under rhizosphere-mimicking conditions using differential rna sequencing (drna-seq). our analysis revealed 4,877 transcription start sites for protein-coding genes, id ... | 2015 | 26540162 |
| exoenzymes and metabolites related to the nematicidal effect of rhizobacteria on xiphinema index thorne & allen. | to identify enzymes and metabolites in the rhizobacteria filtrates that have a nematicidal effect on xiphinema index and perform molecular characterization of the strains evaluated. | 2016 | 26541369 |
| development of a model protein interaction pair as a benchmarking tool for the quantitative analysis of 2-site protein-protein interactions. | a significant challenge in the molecular interaction field is to accurately determine the stoichiometry and stepwise binding affinity constants for macromolecules having >1 binding site. the mission of the molecular interactions research group (mirg) of the association of biomolecular resource facilities (abrf) is to show how biophysical technologies are used to quantitatively characterize molecular interactions, and to educate the abrf members and scientific community on the utility and limitat ... | 2015 | 26543437 |
| diversity and antimicrobial potential of bacterial isolates associated with the soft coral alcyonium digitatum from the baltic sea. | it is well recognized that microorganisms associated with marine invertebrates, in particular sponges and hard corals, are an excellent source of new natural products. therefore, the diversity of bacteria associated with marine invertebrates and their potential to produce bioactive compounds have received much attention in recent years. we report here for the first time on the biodiversity of bacteria associated with the soft coral alcyonium digitatum, which is abundant in the baltic sea. in ord ... | 2016 | 26558794 |
| draft genome sequence of bacillus amyloliquefaciens xk-4-1, a plant growth-promoting endophyte with antifungal activity. | here, we report the draft genome sequence of a bacterial plant-growth-promoting endophyte, bacillus amyloliquefaciens xk-4-1, which consists of one circular chromosome of 3,941,805 bp with 3,702 coding sequences (cdss). the data presented highlight multiple sets of functional genes associated with its plant-beneficial characteristics. | 2015 | 26564038 |
| [degradation of lignocellulose in the corn straw by bacillus amyloliquefaciens mn-8]. | microbial degradation of lignocellulose is one of the key problems that need to be solved urgently in the process of utilizing biomass resource. bacillus amyloliquefaciens mn-8 is our previously isolated bacterium capable of degrading lignin. to determine the capability of strain mn-8 to degrade lignocellulose of corn straw, b. amyloliquefaciens mn-8 was inoculated and fermented with solid-state corn straw powder-msm culture medium. the changes in the enzyme activity and degradation products of ... | 2015 | 26571658 |
| expression of essential genes for biosynthesis of antimicrobial peptides of bacillus is modulated by inactivated cells of target microorganisms. | certain bacillus strains are important producers of antimicrobial peptides with great potential for biological control. antimicrobial peptide production by bacillus amyloliquefaciens p11 was investigated in the presence of heat-inactivated cells of bacteria and fungi. b. amyloliquefaciens p11 exhibited higher antimicrobial activity in the presence of inactivated cells of staphylococcus aureus and aspergillus parasiticus compared to other conditions tested. expression of essential genes related t ... | 2016 | 26577655 |
| high-quality draft genome sequence of bacillus amyloliquefaciens strain 629, an endophyte from theobroma cacao. | bacillus amyloliquefaciens strain 629 is an endophyte isolated from theobroma cacao l. here, we report the draft genome sequence (3.9 mb) of b. amyloliquefaciens strain 629 containing 16 contigs (3,903,367 bp), 3,912 coding sequences, and an average 46.5% g+c content. | 2015 | 26586881 |
| genome sequence of the banana plant growth-promoting rhizobacterium bacillus amyloliquefaciens bs006. | bacillus amyloliquefaciens is an important plant growth-promoting rhizobacterium (pgpr). we report the first whole-genome sequence of pgpr bacillus amyloliquefaciens evaluated in colombian banana plants. the genome sequences encode genes involved in plant growth and defense, including bacteriocins, ribosomally synthesized antibacterial peptides, in addition to genes that provide resistance to toxic compounds. | 2015 | 26607897 |
| control of agitation and aeration rates in the production of surfactin in foam overflowing fed-batch culture with industrial fermentation. | bacillus amyloliquefaciens fmb50 produces a high yield of surfactin, a lipopeptide-type biosurfactant that has been widely studied and has potential applications in many fields. a foam overflowing culture has been successfully used in the combined production-enrichment fermentation of surfactin. in this study, the agitation and aeration rates were found to have relationships with foam formation and surfactin enrichment. a maximum surfactin concentration of 4.7g/l of foam was obtained after 21h o ... | 2016 | 26655454 |
| selection of bacillus spp. for cellulase and xylanase production as direct-fed microbials to reduce digesta viscosity and clostridium perfringens proliferation using an in vitro digestive model in different poultry diets. | previously, our laboratory has screened and identified bacillus spp. isolates as direct-fed microbials (dfm). the purpose of the present study was to evaluate the cellulase and xylanase production of these isolates and select the most appropriate bacillus spp. candidates for dfm. furthermore, an in vitro digestive model, simulating different compartments of the gastrointestinal tract, was used to determine the effect of these selected candidates on digesta viscosity and clostridium perfringens p ... | 2015 | 26664954 |
| construction of a rapid feather-degrading bacterium by overexpression of a highly efficient alkaline keratinase in its parent strain bacillus amyloliquefaciens k11. | keratinase is essential to degrade the main feather component, keratin, and is of importance for wide industrial applications. in this study, bacillus amyloliquefaciens strain k11 was found to have significant feather-degrading capacity (completely degraded whole feathers within 24 h). the keratinase encoding gene, kerk, was expressed in bacillus subtilis sck6. the purified recombinant kerk showed optimal activity at 50 °c and ph 11.0 and degraded whole feathers within 0.5 h in the presence of d ... | 2016 | 26671753 |
| molecular and enzymatic characterization of alkaline lipase from bacillus amyloliquefaciens e1pa isolated from lipid-rich food waste. | bacillus amyloliquefaciens e1pa is a lipase-producing strain that was originally isolated from lipid-rich food waste, and the production of its lipase was found to be induced by vegetable oils. the e1pa lipase was successfully expressed and secreted in a heterologous escherichia coli host and was ultimately purified. the conserved pentapeptide motif ala-his-ser-met-gly was observed at positions 108-112. the purified recombinant lipase was stable over a ph range of 4.0-11.0 at 40 °c and exhibited ... | 2016 | 26672445 |
| computational based functional analysis of bacillus phytases. | phytase is an enzyme which catalyzes the total hydrolysis of phytate to less phosphorylated myo-inositol derivatives and inorganic phosphate and digests the undigestable phytate part present in seeds and grains and therefore provides digestible phosphorus, calcium and other mineral nutrients. phytases are frequently added to the feed of monogastric animals so that bioavailability of phytic acid-bound phosphate increases, ultimately enhancing the nutritional value of diets. the bacillus phytase i ... | 2016 | 26672917 |
| a consortium of rhizobacterial strains and biochemical growth elicitors improve cold and drought stress tolerance in rice (oryza sativa l.). | in the present study, a consortium of two rhizobacteria bacillus amyloliquefaciens bk7 and brevibacillus laterosporus b4, termed 'bb', biochemical elicitors salicylic acid and β-aminobutyric acid (sb) and their mixture (bbsb) were investigated for cold and drought stress tolerance in rice plants. after withholding water for 16 days, rice plants treated with bbsb showed 100% survival, improved seedling height (35.4 cm), shoot number (6.12), and showed minimum symptoms of chlorosis (19%), wilting ... | 2016 | 26681628 |
| [construction and evaluation of an engineered bacterial strain for producing lipopeptide under anoxic conditions]. | biosurfactant-facilitated oil recovery is one of the most important aspects of microbial enhanced oil recovery (meor). however, the biosurfactant production by biosurfactant-producing microorganisms, most of which are aerobes, is severely suppressed due to the in-situ anoxic conditions within oil reservoirs. in this research, we successfully engineered a strain jd-3, which could grow rapidly and produce lipopeptide under anoxic conditions, by protoplast confusion using a bacillus amyloliquefacie ... | 2015 | 26685621 |
| antagonistic effects of bacillus subtilis subsp. subtilis and b. amyloliquefaciens against macrophomina phaseolina: sem study of fungal changes and uv-maldi-tof ms analysis of their bioactive compounds. | the antifungal effect of bacillus subtilis subsp. subtilis pgpmori7 and bacillus amyloliquefaciens pgpbacca1 was evaluated against macrophomina phaseolina (tassi) goid. cell suspension (cs), cell-free supernatant (cfs) and the lipopeptide fraction (lf) of pgpmori7 and pgpbacca1 were screened against three different m. phaseolina strains. cs exhibited the highest inhibitory effect (around 50%) when compared to those of cfs and lf, regardless of the fungal strain studied. the synthesis of lipopept ... | 2016 | 26686611 |
| gene cloning, expression, and characterization of the bacillus amyloliquefaciens ps35 lipase. | lipases are enzymes of immense industrial relevance, and, therefore, are being intensely investigated. in an attempt to characterize lipases at molecular level from novel sources, a lipase gene from bacillus amyloliquefaciens ps35 was cloned, heterologously expressed in escherichia coli dh5α cells and sequenced. it showed up to 98% homology with other lipase sequences in the ncbi database. the recombinant enzyme was then purified from e. coli culture, resulting in a 19.41-fold purification with ... | 2017 | 26691486 |
| novel routes for improving biocontrol activity of bacillus based bioinoculants. | biocontrol (bc) formulations prepared from plant-growth-promoting bacteria are increasingly applied in sustainable agriculture. especially inoculants prepared from endospore-forming bacillus strains have been proven as efficient and environmental-friendly alternative to chemical pesticides due to their long shelf life, which is comparable with that of agrochemicals. however, these formulations of the first generation are sometimes hampered in their action and do not fulfill in each case the expe ... | 2015 | 26696998 |
| bacillus velezensis is not a later heterotypic synonym of bacillus amyloliquefaciens; bacillus methylotrophicus, bacillus amyloliquefaciens subsp plantarum and 'bacillus oryzicola' are later heterotypic synonyms of bacillus velezensis based on phylogenomics. | bacillus velezensis was previously reported to be a later heterotypic synonym of bacillus amyloliquefaciens, based on dna-dna relatedness values. we have sequenced a draft genome of b. velezensis nrrl b-41580t. comparative genomics and dna-dna relatedness calculations show that it is not a synonym of b. amyloliquefaciens. it was instead synonymous with bacillus methylotrophicus. 'bacillus oryzicola' is a recently described species that was isolated as an endophyte of rice (oryza sativa). the str ... | 2015 | 26702995 |
| bacillus amyloliquefaciens biofilm as a novel biosorbent for the removal of crystal violet from solution. | bacillus amyloliquefaciens biofilm shows promise for use in the control of soil-borne pathogens; however, it has never been used to treat dye-polluted wastewaters. here, we propose the novel idea of using b. amyloliquefaciens biofilm for the adsorption of crystal violet (cv) from liquids. the relative contents of three main elements (c1s, o1s, and n1s) in the biofilm were 65.55%, 21.21%, and 13.24%, respectively. the results of fourier transform infrared (ftir) spectra and x-ray photoelectron sp ... | 2016 | 26707697 |
| the antibiosis action and rice-induced resistance, mediated by a lipopeptide from bacillus amyloliquefaciens b014, in controlling rice disease caused by xanthomonas oryzae pv. oryzae. | in the present study, a lipopeptide (named axlp14) antagonistic to xanthomonas oryzae pv. oryzae (xoo) was obtained from the culture supernatant of bacillus amyloliquefaciens b014. matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis demonstrated that axlp14 consisted of surfactin homologs. the minimum inhibition concentration and minimum bactericidal concentration of axlp14 against xoo were determined to be 1.25 and 2.50 mg/ml, respectively. at a concentration o ... | 2016 | 26718470 |
| optimization of medium components for production of chitin deacetylase by bacillus amyloliquefaciens z7, using response surface methodology. | plackett-burman design and box-behnken response surface methodology (rsm) was employed to optimize the medium components for the chitin deacetylase (cda) activity from bacillus amyloliquefaciens z7. plackett-burman design was applied to determine the specific medium components affecting cda activity and found that starch, chitin and mgso4 were critical in augmenting cda activity. these significant parameters were further optimized using box-behnken rsm and the optimum concentrations of starch, c ... | 2014 | 26740755 |
| uv photolysis for enhanced phenol biodegradation in the presence of 2,4,6-trichlorophenol (tcp). | a bacterial strain isolated from activated sludge and identified as bacillus amyloliquefaciens could biodegrade phenol, but 2,4,6-trichlorophenol (tcp) inhibited phenol biodegradation and biomass growth. uv photolysis converted tcp into dichlorocatechol, monochlorophenol, and dichlorophenol, and this relieved inhibition by tcp. phenol-removal and biomass-growth rates were significantly accelerated after uv photolysis: the monod maximum specific growth rate (μ(max)) increased by 9% after tcp phot ... | 2016 | 26747251 |
| engineering of a bacillus amyloliquefaciens strain with high neutral protease producing capacity and optimization of its fermentation conditions. | the neutral protease has high potential for industrial applications, and attempts to improve enzyme expression level have important application values. in the present study, a neutral protease-encoding gene, banpr, was cloned from bacillus amyloliquefaciens strain k11, and a genetic manipulation method specific for this difficult-to-transform strain was developed for the high-level expression of neutral protease. the recombinant plasmid pub110-banpr was constructed in bacillus subtilis strain wb ... | 2016 | 26752595 |
| [screening and antibacterial function of bacillus amyloliquefaciens x030]. | we isolated 339 bacillus strains from 72 soil samples all over the country, then purified their antimicrobial compounds and studied the antibacterial activity, to enrich bacillus resources and explore their second metabolites. | 2015 | 26762026 |
| biological control activities of rice-associated bacillus sp. strains against sheath blight and bacterial panicle blight of rice. | potential biological control agents for two major rice diseases, sheath blight and bacterial panicle blight, were isolated from rice plants in this study. rice-associated bacteria (rabs) isolated from rice plants grown in the field were tested for their antagonistic activities against the rice pathogens, rhizoctonia solani and burkholderia glumae, which cause sheath blight and bacterial panicle blight, respectively. twenty-nine rabs were initially screened based on their antagonistic activities ... | 2016 | 26765124 |
| antilisterial activity on poultry meat of amylolysin, a bacteriocin from bacillus amyloliquefaciens ga1. | this paper describes the production, the purification and the antilisterial activity of amylolysin, a novel bacteriocin from b. amyloliquefaciens ga1. the strain genome was first analysed using pcr techniques for the presence of gene clusters that direct the synthesis of characterised bacteriocins from b. amyloliquefaciens and the closely related b. subtilis. our results suggest that amylolysin corresponds to a novel bacteriocin. the effect of amylolysin on the growth of different isolates of li ... | 2010 | 26781121 |
| antibacterial activity of subtilosin alone and combined with curcumin, poly-lysine and zinc lactate against listeria monocytogenes strains. | in this paper, the antibacterial effects of the bacillus amyloliquefaciens-produced bacteriocin subtilosin, both alone and in combination with curcumin, ε-poly-l-lysine (poly-lysine), or zinc lactate, were examined against listeria monocytogenes. results indicated that subtilosin inhibits both of the studied bacterial strains, scott a (wild-type, nisin sensitive) and nr30 (nisin resistant). however, l. monocytogenes scott a was more sensitive to subtilosin and pure curcumin. in addition, subtilo ... | 2010 | 26781320 |
| isolation and characterization of putative probiotic bacterial strain, bacillus amyloliquefaciens, from north east himalayan soil based on in vitro and in vivo functional properties. | this study is aimed to isolate some novel probiotics from the soils of north east himalayas. eleven gram positive isolates were obtained in mrs with oxgall media from soil samples. four of the isolates withstood the in vitro gastric juice ph 3.0 and 0.45% bile salt tolerance screening. among these, pbt 3 showed high cell surface hydrophobicity and adhered to the caco-2 cells. 16s rdna gene sequences of this probiotic strains were identified as bacillus amyloliquefaciens (accession no: jf836079). ... | 2011 | 26781678 |
| antimicrobial activity of bacillus amyloliquefaciens ant1 toward pathogenic bacteria and mold: effects on biofilm formation. | the intensive use and misuse of antibiotics over the last decades have generated a strong selective pressure for the emergence of multi-resistant strains and nosocomial infections. biofilm has been demonstrated as a key parameter in spreading infections, especially in hospitals and healthcare units. therefore, the development of novel anti-biofilm drugs is actually of the upmost importance. here, the antimicrobial and antibiofilm activities toward pathogenic microorganisms of a set of non-riboso ... | 2013 | 26783071 |
| plant-microbe communication enhances auxin biosynthesis by a root-associated bacterium, bacillus amyloliquefaciens sqr9. | mechanisms by which beneficial rhizobacteria promote plant growth include tryptophan-dependent indole-3-acetic acid (iaa) synthesis. the abundance of tryptophan in the rhizosphere, however, may influence the level of benefit provided by iaa-producing rhizobacteria. this study examined the cucumber-bacillus amyloliquefaciens sqr9 system and found that sqr9, a bacterium previously shown to enhance the growth of cucumber, increased root secretion of tryptophan by three- to fourfold. using a split-r ... | 2016 | 26808445 |
| constitutive production and efficient secretion of soluble full-length streptavidin by an escherichia coli 'leaky mutant'. | due to its various applications the protein streptavidin is a highly interesting target for heterologous production. this study focuses on different escherichia coli-based constructs targeting a high-level expression and secretion of streptavidin to the medium. the effect of various promoters, variants of the target gene, leader sequences and host strains on expression and secretion into the culture broth was analyzed. constitutive production of full-length streptavidin fused with the leader seq ... | 2016 | 26820322 |
| acetylome analysis reveals the involvement of lysine acetylation in biosynthesis of antibiotics in bacillus amyloliquefaciens. | lysine acetylation is a major post-translational modification that plays an important regulatory role in almost every aspects in both eukaryotes and prokaryotes. bacillus amyloliquefaciens, a gram-positive bacterium, is very effective for the control of plant pathogens. however, very little is known about the function of lysine acetylation in this organism. here, we conducted the first lysine acetylome in b. amyloliquefaciens through a combination of highly sensitive immune-affinity purification ... | 2016 | 26822828 |