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antibacterial effect of hydroalcoholic extract of punica granatum linn. petal on common oral microorganisms.objectives. this study aimed to assess the effect of hydroalcoholic extract of punica granatum linn. (p. granatum) petal on streptococcus sanguinis, streptococcus mutans, streptococcus salivarius, streptococcus sobrinus, and enterococcus faecalis. materials and methods. in this in vitro study, p. granatum extract was prepared using powdered petals and water-ethanol solvent. antibacterial effect of the extract, chlorhexidine (chx), and ampicillin was evaluated on brain heart infusion agar (bhia) ...201626884763
pcr-dipstick dna chromatography for profiling of a subgroup of caries-associated bacterial species in plaque from healthy coronal surfaces and periodontal pockets.the onset of plaque-mediated disease, including dental caries and periodontal diseases, is highly associated with compositional change of the resident microflora from the ecological perspective. as specific bacterial profiles have been linked to different disease stages, microbial compositional measurements might therefore have great value for clinical diagnosis. previously we have reported a dry-reagent strip biosensor-pcr-dipstick dna chromatography, which utilized molecular recognition of oli ...201626912138
the comparative evaluation of the antimicrobial effect of propolis with chlorhexidine against oral pathogens: an in vitro study.this study aimed to compare the antimicrobial effectiveness of ethanolic extract of propolis (eep) to chlorhexidine gluconate (chx) on planktonic streptococcus mutans, streptococcus sobrinus, lactobacillus acidophilus, lactobacillus salivarius subsp. salivarius, aggregatibacter actinomycetemcomitans, prevotella intermedia, porphyromonas gingivalis, staphylococcus aureus, enterococcus faecalis, actinomyces israelii, candida albicans, and their single-species biofilms by agar dilution and broth mi ...201626949701
frequency of dental caries in active and inactive systemic lupus erythematous patients: salivary and bacterial factors.the objective of this study was to determine dental caries frequency and to analyze salivary and bacterial factors associated with active and inactive systemic lupus erythematous (sle) patients. also, a proposal to identify dental caries by a surface, teeth, and the patient was developed.201627053402
galla chinensis compounds remineralize enamel caries lesions in a rat model.to investigate the effect of galla chinensis chemical compounds on enamel caries remineralization in rats.201627111668
inhibitory effects of ellagic acid on glucosyltransferases from mutans streptococci.ellagic acid and its derivatives were found to abrogate the enzymatic activity of the glucosyltransferases (gtases) of mutans streptococci. these compounds preferentially inhibited the water-soluble glucan synthesis by gtase of streptococcus mutans mt8148 and inhibited the water-insoluble glucan synthesis by gtase of streptococcus sobrinus 6715. this inhibition resulted in a reduction of cellular adherence of mutans streptococci to a glass surface.199227286205
evaluation of antibacterial efficiency of chitosan and chitosan nanoparticles on cariogenic streptococci: an in vitro study.the most prevalent and worldwide oral disease is dental caries that affects a significant proportion of the world population. there are some classical approaches for control, prevention and treatment of this pathologic condition; however, the results are still not completely successful. therefore new methods are needed for better management of this important challenge. chitosan is a natural and non-toxic polysaccharide with many biological applications, particularly as an antimicrobial agent. ch ...201627307974
differential utilization of basic proline-rich glycoproteins during growth of oral bacteria in saliva.although saliva is widely recognized as a primary source of carbon and nitrogen for growth of the dental plaque biofilm community, little is known about how different oral bacteria utilize specific salivary components. to address this question, 32 strains representing 16 genera commonly isolated from early plaque biofilms were compared for growth over two transfers in stimulated (by chewing parafilm) whole saliva that was stabilized by heat treatment and dialysis. the cell densities, measured by ...201627316966
influence of sucrose and xylitol on an early streptococcus mutans biofilm in a dental simulator.in vitro methods to study dental biofilms are useful in finding ways to support a healthy microbial balance in the oral cavity. the effects of sucrose, xylitol, and their combination on three strains of streptococcus mutans and one strain of streptococcus sobrinus were studied using a dental simulator.201627318453
pleurocidin congeners demonstrate activity against streptococcus and low toxicity on gingival fibroblasts.fish epidermal antimicrobial peptides, such as pleurocidin, are cathelicidins with broad-spectrum antimicrobial activity against gram negative and gram-positive bacteria, as well as fungi. in the current study, we attempted to optimize peptide bioactivity by sequence modification and assess the antimicrobial activities.201627341459
streptococcus mutans, streptococcus sobrinus and candida albicans in oral samples from caries-free and caries-active children.this was to examine the occurrence of s. mutans, s. sobrinus and c. albicans in dental plaque and saliva from caries-free and caries-active greek children.201627357362
microbial ecosystem analysis in root canal infections refractory to endodontic treatment.the purpose of this study was to combine multiple displacement amplification and checkerboard dna-dna hybridization to qualitatively and quantitatively evaluate the microbiota present in infections refractory to endodontic treatment.201627377440
streptococcusdentiloxodontae sp. nov., isolated from the oral cavity of elephants.a gram-stain-positive, catalase-negative, coccus-shaped organism was isolated from oral cavity samples collected from healthy elephants. the isolated strain, num 2404t, was tentatively identified as a streptococcal species based on the results of biochemical tests. although a comparative 16s rrna gene sequence analysis suggested the classification of this organism into the genus streptococcus, it did not correspond to any recognized species of the genus. strain num 2404t was related most closely ...201627381035
inhibition of glucan synthesis by casein polymers crosslinked by glutaraldehyde.glutaraldehyde-crosslinked α-casein polymers with molecular masses of 1-5×10(5) were found to inhibit adhesive insoluble glucan formation catalyzed by glucan synthases from streptococcus sobrinus b13, a cariogenic oral bacterium. of the three subcomponents of the glucan synthases tested, primer-dependent insoluble glucan synthase was inhibited specifically by the m r~2.7×10(5) polymer at a concentration of 1.4-8.5 μg/ml. the polymer was also active in inhibiting the artificial plaque formation b ...199827393366
synthesis and screening of n-acyl thiolated chitosans for antibacterial applications.low-molecular weight chitosan-thioglycolic acid has shown significant antibacterial properties against different microorganisms. in order to explore the potential and structure-activity relationships of newly synthesized alkyl thiomers, chitosan has been functionalized with a series of thio-acids with increasing alkyl chain length. all thiomers were characterized with special emphasis on the determination of their degree of deacetylation and substitution, as well as on their molecular weight and ...201627474669
comparative in vitro investigation of the cariogenic potential of bifidobacteria.this study aimed to assess the in vitro cariogenic potential of some bifidobacterium species in comparison with caries-associated bacteria.201627475723
the effect of seasons on brazilian red propolis and its botanical source: chemical composition and antibacterial activity.the aim of this study was to evaluate the effect of seasons on the chemical composition and antibacterial activity of brazilian red propolis (brp) and its plant source. brp was collected from maceio, alagoas state, north-east of brazil, during one year. chemical composition was determined by physicochemical analyses and hplc while antimicrobial activity was assessed against streptococcus mutans, streptococcus sobrinus, staphylococcus aureus and actinomyces naeslundii by determining the minimal i ...201727701899
no evidence for the growth-stimulating effect of monomers on cariogenic streptococci.in spite of contradicting results, the high susceptibility of composites for secondary caries is still often associated with the bacterial growth-stimulating effect of released methacrylate monomers. however, most studies that showed this effect were performed with techniques having inherent limitations (spectrophotometry).201627766489
comparative study on the characteristics of weissella cibaria cmu and probiotic strains for oral care.probiotics have been demonstrated as a new paradigm to substitute antibiotic treatment for dental caries, gingivitis, and chronic periodontitis. the present work was conducted to compare the characteristics of oral care probiotics: weissella cibaria cmu (chonnam medical university) and four commercial probiotic strains. survival rates under poor oral conditions, acid production, hydrogen peroxide production, as well as inhibition of biofilm formation, coaggregation, antibacterial activity, and i ...201627999400
five-year longitudinal study of dental caries risk associated with streptococcus mutans and streptococcus sobrinus in individuals with intellectual disabilities.streptococcus mutans (s. mutans) and streptococcus sobrinus (s. sobrinus) are important etiologic agents in human dental caries. using quantitative real-time polymerase chain reaction assays for the presence of those strains, we examined 145 outpatients with intellectual disability (id), calculated the proportion of each of these strains to total bacteria, and compared dental caries incidence over 5 years. plaque samples were collected from all erupted tooth sites, and dental examinations were p ...201728049966
distribution of streptococcus mutans and streptococcus sobrinus in dental plaque of indian pre-school children using pcr and sb-20m agar medium.dental caries is one of the most common infectious diseases affecting the oral cavity. among the oral bacteria, mutans streptococci have been implicated as major cariogenic bacteria as they can produce high levels of dental caries causing substances such as lactic acid and extracellular polysaccharides.201628050506
evaluation of the antibacterial effects of aqueous and ethanolic leaf extracts of aloysia citriodora (lemon verbena) on streptococcus mutans and streptococcus sobrinus.the aloysia citriodora plant from the family of verbenaceae has many uses in traditional medicine. the aim of the current study was to determine the effects of the aqueous and ethanolic extracts of a. citriodora on streptococcus mutans and streptococcus sobrinus, which cause tooth decay.201628163849
[influence of surface roughness on oral streptococcal adhesion forces to dental filling materials].this study is to determine the common oral streptococcal adhesion forces by using composite resin and glass ionomer cement (gic) with different degrees of surface roughness via atomic force microscopy (afm) analysis. the influence of surface roughness on bacterial adhesion force is also discussed.201628326699
compositional differences in multi-species biofilms formed on various orthodontic adhesives.to investigate the aspects of multi-species biofilm formation on various orthodontic adhesives with different surface characteristics.201728339597
chemical composition, antibacterial, schistosomicidal, and cytotoxic activities of the essential oil of dysphania ambrosioides (l.) mosyakin & clemants (chenopodiaceae).we have investigated the chemical composition and the antibacterial activity of the essential oil of dysphania ambrosioides (l.) mosyakin & clemants (chenopodiaceae) (da-eo) against a representative panel of cariogenic bacteria. we have also assessed the in vitro schistosomicidal effects of da-eo on schistosoma mansoni and its cytotoxicity to gm07492-a cells in vitro. gas chromatography (gc) and gas chromatography-mass spectrometry (gc/ms) revealed that the monoterpenes cis-piperitone oxide (35. ...201728504841
in-vitro efficacy of different morphology zinc oxide nanopowders on streptococcus sobrinus and streptococcus mutans.zno with two different morphologies were used to study the inhibition of streptococcus sobrinus and streptococcus mutans which are closely associated with tooth cavity. rod-like shaped zno-a and plate-like shaped zno-b were produced using a zinc boiling furnace. the nanopowders were characterized using energy filtered transmission electron microscopy (eftem), x-ray diffraction (xrd), photoluminescence (pl) spectroscopy, raman spectroscopy and dynamic light scattering (dls) to confirm the propert ...201728576061
improved method for rapid and accurate isolation and identification of streptococcus mutans and streptococcus sobrinus from human plaque samples.mutans streptococci (ms), specifically streptococcus mutans (sm) and streptococcus sobrinus (ss), are bacterial species frequently targeted for investigation due to their role in the etiology of dental caries. differentiation of s. mutans and s. sobrinus is an essential part of exploring the role of these organisms in disease progression and the impact of the presence of either/both on a subject's caries experience. of vital importance to the study of these organisms is an identification protoco ...201728606792
antimicrobial and biological activity of leachate from light curable pulp capping materials.characterization of a number of pulp capping materials and assessment of the leachate for elemental composition, antimicrobial activity and cell proliferation and expression.201728645637
effect of arginine on the growth and biofilm formation of oral bacteria.alkali production via arginine deiminase system (ads) of oral bacteria plays a significant role in oral ecology, ph homeostasis and inhibition of dental caries. ads activity in dental plaque varies greatly between individuals, which may profoundly affect their susceptibility to caries.201728668766
the oral bacterial microbiome of occlusal surfaces in children and its association with diet and caries.dental caries is the most prevalent disease in humans globally. efforts to control it have been invigorated by an increasing knowledge of the oral microbiome composition. this study aimed to evaluate the bacterial diversity in occlusal biofilms and its relationship with clinical surface diagnosis and dietary habits. anamneses were recorded from thirteen 12-year-old children. biofilm samples collected from occlusal surfaces of 46 permanent second molars were analyzed by 16s rrna amplicon sequenci ...201728678838
salivary density of streptococcus mutans and streptococcus sobrinus and dental caries in children and adolescents with down syndrome.to assess and compare dental caries experience and salivary s. mutans, s. sobrinus, and streptococci counts between groups of down syndrome and non-down syndrome children and adolescents.201728678943
characterization and antimicrobial efficacy of portland cement impregnated with silver nanoparticles.this study investigated the effects of silver nanoparticle (sn) loading into hydraulic calcium silicate-based portland cement on its mechanical, antibacterial behavior and biocompatibility as a novel dental bone substitute.201728680554
oral microbial profiles of individuals with different levels of sugar intake.the aim was to compare the oral microbial profiles in young adults with an intake of free sugars above or below the current recommendations by the who for sugar consumption. seventy subjects completed a quantitative food frequency questionnaire to establish the proportion of free sugars in relation to the total energy intake (% e). subjects with <5% e (n = 30) formed the low-sugar group, while those with ≥5% e (n = 40) were regarded as reference group. saliva and plaque samples were analyzed by ...201728839520
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