Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| alteration of bile acid metabolism by cimetidine in healthy humans. | to clarify an effect of cimetidine on bile acid metabolism, we evaluated whether an increased deconjugation of bile acids would occur in healthy humans who have received cimetidine. we examined: 1) whether healthy volunteers taking cimetidine would have positive bile acid breath tests because of bacterial overgrowth in the jejunum; 2) whether the isolated bacteria would exhibit deconjugation ability; and 3) whether a change in gastric ph was related to the bacterial overgrowth. | 1996 | 8952227 |
| conjugal transfer of the 5-nitroimidazole resistance plasmid pip417 from bacteroides vulgatus bv-17: characterization and nucleotide sequence analysis of the mobilization region. | three small 5-nitroimidazole (5-ni) resistance plasmids (pip417, pip419, and pip421) from bacteroides clinical isolates are transferable by a conjugative process during homologous or heterologous matings. the mobilization properties of pip417 originated from strain bv-17 of bacteroides vulgatus were studied. the plasmid was successfully introduced by in vitro conjugation into different strains of bacteroides and prevotella species and could be transferred back from these various strains to a pla ... | 1996 | 8955281 |
| antibacterial activities of tosufloxacin against anaerobic bacteria and the electron micrograph of its bactericidal effects. | tosufloxacin, a quinolone, showed a broad antibacterial spectrum against gram-positive and gram-negative bacteria including anaerobic bacteria. tosufloxacin was 4- to 8-fold more active than levofloxacin and ciprofloxacin. the mic90 of tosufloxacin for clinical isolates of bacteroides fragilis, bacteroides vulgatus, bacteroides thetaiotaomicron and peptostreptococcus asaccharolyticus were 0.78, 0.39, 1.56 and 0.39 micrograms/ml, respectively. morphological observation with the scanning and trans ... | 1997 | 9142454 |
| distribution and mobility of the tetracycline resistance determinant tetq. | we tested 34 american type culture collection (atcc) and 168 clinical bacterial isolates, from the human urogenital and oral tracts and streptococci isolated from cows with mastitis, for the presence of the tetq gene using a polymerase chain reaction (pcr) assay and dna-dna hybridization. the identities of pcr products were confirmed by southern blot hybridization of whole-cell dna. eleven of the atcc strains were positive for tetq, including five bacteroides spp., five prevotella spp. and a sin ... | 1997 | 9372425 |
| omeprazole induces altered bile acid metabolism. | it has been reported that the acidity of gastric contents could be an important factor in regulating jejunal flora. | 1998 | 9536953 |
| immunochemical characterization of bacteroides vulgatus cell-surface antigens. | capsular polysaccharide (cps) and lipopolysaccharide (lps) antigens extracted from three bacteroides vulgatus strains (serotype specific strain, and two others isolated from normal gut flora and gut of a diabetic patient) were studied. chemical analysis of cps and purified lps antigens was performed. the compositions of sugars, amino acids and fatty acids were shown to be different for each antigen. rabbit antisera against whole bacterial cells were prepared. all antigens proved to be active in ... | 1998 | 9691430 |
| resident enteric bacteria are necessary for development of spontaneous colitis and immune system activation in interleukin-10-deficient mice. | mice with targeted deletion of the gene for interleukin-10 (il-10) spontaneously develop enterocolitis when maintained in conventional conditions but develop only colitis when kept in specific-pathogen-free (spf) environments. this study tested the hypothesis that enteric bacteria are necessary for the development of spontaneous colitis and immune system activation in il-10-deficient mice. il-10-deficient mice were maintained in either spf conditions or germfree conditions or were populated with ... | 1998 | 9784526 |
| deconjugation ability of bacteria isolated from the jejunal fluid of patients with progressive systemic sclerosis and its gastric ph. | our goal was to demonstrate the role of bacteria in altered bile acid metabolism, which overgrow in the upper small intestine of patients with progressive systemic sclerosis. we identified the bacterial species, isolated from the jejunal fluid obtained from patients with progressive systemic sclerosis, who had previously shown an increase in 14co2, specific activity on breath test, and normal controls. after which, we investigated the deconjugation ability of the isolated bacteria and the relati ... | 1998 | 9840121 |
| dermoid cyst with dermal sinus tract complicated with spinal subdural abscess. | spinal subdural abscess caused by spread of infection with the dermal sinus tract is rare in children. this article reports on a 1-year-old male with prolonged fever, progressive paraplegia, and bowel and bladder dysfunction resulting from a spinal subdural abscess secondary to an infected spinal dermoid cyst with a dermal sinus tract. this is the youngest patient to be reported having this condition. surgical intervention was performed to find a tumor that had capsule and keratinlike contents. ... | 1999 | 10082349 |
| differential induction of colitis and gastritis in hla-b27 transgenic rats selectively colonized with bacteroides vulgatus or escherichia coli. | resident bacteria play an important role in initiating and perpetuating gastrointestinal inflammation. we previously demonstrated that six commensal bacteria including bacteroides vulgatus caused more aggressive colitis and gastritis in hla-b27 transgenic rats than did the other five bacteria without b. vulgatus. this study compared the degree of gastrointestinal inflammation in gnotobiotic hla-b27 transgenic rats monoassociated with either b. vulgatus or escherichia coli. gnotobiotic transgenic ... | 1999 | 10338507 |
| in vitro activity of gemifloxacin (sb 265805) against anaerobes. | gemifloxacin mesylate (sb 265805), a new fluoronaphthyridone, was tested against 359 recent clinical anaerobic isolates by the national committee for clinical laboratory standards reference agar dilution method with supplemented brucella blood agar and an inoculum of 10(5) cfu/spot. comparative antimicrobials tested included trovafloxacin, levofloxacin, grepafloxacin, sparfloxacin, sitafloxacin (du-6859a), penicillin g, amoxicillin clavulanate, imipenem, cefoxitin, clindamycin, and metronidazole ... | 1999 | 10471570 |
| microbes involved in dissimilatory nitrate reduction in the human large intestine. | nitrate-limited batch cultures, incorporating 20 different fermentation substrates and inoculated with human faeces, mainly selected for the growth of enterobacteria. the microbial diversity involved was determined by a combination of phenotypic and genotypic procedures. continuous culture with lactate as the sole electron donor selected for similar micro-organisms, but when antibiotics were incorporated to inhibit escherichia coli and lactate was replaced with choline, there was a wider microbi ... | 2000 | 10620715 |
| characterization of antibody responses against rectal mucosa-associated bacterial flora in patients with ulcerative colitis. | previous reports on faecal microflora have demonstrated that the total number of aerobes and coliforms was increased in patients with ulcerative colitis (uc). based on the hypothesis that the pathogenesis of uc may be closely associated with the mucosal microflora, we investigated alterations in the mucosa-associated microflora of uc patients. | 2000 | 10719749 |
| comparative activity of moxifloxacin in vitro against obligately anaerobic bacteria. | the antimicrobial activity of moxifloxacin and seven other antibiotics (four of them quinolones) against 292 strains of obligately anaerobic bacteria was assessed employing a broth microdilution technique performed in wilkens-chalgren broth. mic50/mic90 values (mg/l) for moxifloxacin were as follows: bacteroides fragilis (n = 62) 0.25/2, bacteroides ovatus (n = 70) 1/4, bacteroides vulgatus (n = 29) 0.25/1, bacteroides thetaiotaomicron (n = 17) 2/2, bacteroides caccae (n = 11) 1/2, prevotella sp ... | 2000 | 10795599 |
| in vitro activity of telithromycin (hmr 3647) and seven other antimicrobial agents against anaerobic bacteria. | we assessed the in vitro activity of telithromycin (hmr 3647) and seven other antimicrobials against 292 strains of obligately anaerobic bacteria. mics were determined with the microdilution technique and wilkins-chalgren broth according to din 58940-83. mic50/mic90s (mg/l) for telithromycin were 4/4 for bacteroides fragilis, bacteroides ovatus and bacteroides thetaiotaomicron, 2/4 for fusobacterium spp. and bilophila wadsworthia, 2/2 for bacteroides caccae, 1/4 for bacteroides vulgatus, 0.25/4 ... | 2000 | 10882699 |
| medical treatment of cholangiohepatitis and cholelithiasis in mature horses: 9 cases (1991-1998). | the medical approach to treatment of cholangiohepatitis and cholelithiasis in 9 horses is described. seven horses were treated successfully and returned to normal use, with a minimum follow-up period of 12 months. long-term antimicrobial therapy was believed to be critical in those cases that survived, with a median treatment duration of 51 days (range 17-124 days). treatment failure was associated with severe periportal and bridging hepatic fibrosis from biopsy material obtained at admission in ... | 2000 | 10952378 |
| preparation and preclinical evaluation of a novel liposomal complete-core lipopolysaccharide vaccine. | our objective is to develop a prophylactic vaccine strategy that can be evaluated for surgical and other high-risk hospitalized patients. in this paper, we describe the preparation and preclinical evaluation of a liposomal complete-core lipopolysaccharide (lps) vaccine that is nontoxic and broadly antigenic. complete-core (ra-chemotype) lpss were isolated from four gram-negative bacterial strains (escherichia coli k-12, e. coli r1, pseudomonas aeruginosa pac608, and bacteroides fragilis), mixed ... | 2000 | 11035726 |
| alteration of v beta usage and cytokine production of cd4+ tcr beta beta homodimer t cells by elimination of bacteroides vulgatus prevents colitis in tcr alpha-chain-deficient mice. | a major pathogenic factor for the development of inflammatory bowel disease (ibd) is the breakdown of the intestinal homeostasis between the host immune system and the luminal microenvironment. to assess the potential influence of luminal ags on the development of ibd, we fed tcr alpha(-/-) mice an elemental diet (ed). ed-fed tcr alpha(-/-) mice showed no pathologic features of ibd, and their aberrant mucosal b cell responses were suppressed. similar numbers of cd4(+), tcr betabeta homodimer t c ... | 2000 | 11067950 |
| comparative in vitro activities of abt-773 against 362 clinical isolates of anaerobic bacteria. | the activity of abt-773, a novel ketolide antibiotic, against clinical isolates of anaerobic bacteria was determined and compared to the activities of other antimicrobial agents. mics at which 90% of isolates were inhibited (mic(90)s) were </=0.06 microg/ml for actinomyces spp., clostridium perfringens, peptostreptococcus spp., propionibacterium spp., and porphyromonas spp. the mic(50)s and mic(90)s were </=0.06 and >32 microg/ml, respectively, for eubacterium spp., lactobacillus spp., clostridi ... | 2001 | 11120995 |
| multicenter survey of the changing in vitro antimicrobial susceptibilities of clinical isolates of bacteroides fragilis group, prevotella, fusobacterium, porphyromonas, and peptostreptococcus species. | in vitro surveys of antimicrobial resistance among clinically important anaerobes are an important source of information that can be used for clinical decisions in the choice of empiric antimicrobial therapy. this study surveyed the susceptibilities of 556 clinical anaerobic isolates from four large medical centers using a broth microdilution method. piperacillin-tazobactam was the only antimicrobial agent to which all the isolates were susceptible. similarly, imipenem, meropenem, and metronidaz ... | 2001 | 11257040 |
| structural elucidation of a capsular polysaccharide from a clinical isolate of bacteroides vulgatus from a patient with crohn's disease. | the structure of a capsular polysaccharide (cps) from a clinical isolate of bacteroides vulgatus was elucidated. b. vulgatus imcj 1204 was isolated from feces of a patient with crohn's disease. cps was prepared by phenol/water extraction of the bacterial cells followed by hydrophobic interaction chromatography and then gel filtration chromatography of the extract. the structure of cps was determined by chemical analysis and nmr spectroscopy including dqf-cosy, tocsy, roesy, hsqc-tocsy, hmqc and ... | 2001 | 11389714 |
| sequence analysis of the plasmid prri2 from the rumen bacterium prevotella ruminicola 223/m2/7 and the use of prri2 in prevotella/bacteroides shuttle vectors. | prri2 is a small cryptic plasmid from the rumen bacterium prevotella ruminicola 223/m2/7 which has been used for the construction of shuttle vectors (prh3 and prri207) that replicate in many bacteroides/prevotella strains as well as in escherichia coli. sequence analysis of prri2 reveals that it is a 3240-bp plasmid carrying two clear open reading frames. rep, encoded by orf1, shows 48 and 47% amino acid sequence identity with repa proteins from bacteroides vulgatus and bacteroides fragilis, res ... | 2001 | 11407918 |
| gyra mutations associated with quinolone resistance in bacteroides fragilis group strains. | mutations in the gyra gene contribute considerably to quinolone resistance in escherichia coli. mechanisms for quinolone resistance in anaerobic bacteria are less well studied. the bacteroides fragilis group are the anaerobic organisms most frequently isolated from patients with bacteremia and intraabdominal infections. forty-four clinafloxacin-resistant and-susceptible fecal and clinical isolates of the b. fragilis group (eight bacteroides fragilis, three bacteroides ovatus, five bacteroides th ... | 2001 | 11408211 |
| cloning and biochemical characterization of a class a beta-lactamase from prevotella intermedia. | the gene encoding a beta-lactamase of prevotella intermedia was cloned and sequenced. this gene, called cfxa2, shared 98% identity with cfxa, the structural gene of a beta-lactamase previously described in bacteroides vulgatus. the deduced protein sequence had a k272e substitution. cfxa2 had the characteristics of class a, group 2e beta-lactamases. | 2001 | 11451705 |
| production and application of new monoclonal antibodies specific for a fecal helicobacter pylori antigen. | the aim of the present study was to establish monoclonal antibodies that could be used to produce a diagnostic test composed of one kind of monoclonal antibody recognizing a fecal helicobacter pylori antigen. the need to develop such a test arose from disadvantages of the diagnostic test that uses a polyclonal antibody or plural kinds of monoclonal antibodies, such as the lower specificity for h. pylori antigen and the difficulty of reproduction with consistent quality. mice were immunized with ... | 2002 | 11777832 |
| adhesive property of bifidobacterium lactis lkm512 and predominant bacteria of intestinal microflora to human intestinal mucin. | the adhesive property to the intestinal mucin of bifidobacterium lactis lkm512, b. longum, b. breve, b. bifidum, b. adolescentis, b. infantis, bacteroides vulgatus, bacteroides distasonis, eubacterium aerofaciens, clostridium perfringens, escherichia coli, and lactobacillus acidophilus were examined. adhesive rate of lkm512 to the mucin was significantly (p < 0.05, 0.01, or 0.001) stronger than the other strains from 2 to 100 time. though the adhesive property of many strains was almost same to ... | 2002 | 11821930 |
| accelerated alveolar bone loss in hla-b27 transgenic rats: an adult onset condition. | patients with arthritis and crohn's disease may be more susceptible to periodontitis associated alveolar bone loss (abl). hla-b27 transgenic (tg) rats spontaneously develop arthritis and colitis. based on the hypothesis that tg rats would also be susceptible to abl, we compared the naturally occurring abl in tg and fischer 344 wild-type (wt) rats. | 2002 | 12064843 |
| an oral introduction of intestinal bacteria prevents the development of a long-term th2-skewed immunological memory induced by neonatal antibiotic treatment in mice. | recent epidemiological studies indicate that antibiotic use in infancy may be associated with an increased risk of developing atopy. our previous work on animals demonstrated that kanamycin use during infancy promotes a shift in the th1/th2 balance towards a th2-dominant immunity. | 2002 | 12100062 |
| ikk beta and phosphatidylinositol 3-kinase/akt participate in non-pathogenic gram-negative enteric bacteria-induced rela phosphorylation and nf-kappa b activation in both primary and intestinal epithelial cell lines. | pathogenic and enteroinvasive bacteria have been shown to trigger the i kappa b/nf-kappa b transcriptional system and proinflammatory gene expression in epithelial cells. in this study, we investigated the molecular mechanism of the commensal gram-negative bacteroides vulgatus-induced nf-kappa b signal transduction in intestinal epithelial cells (iec). we report that b. vulgatus induced interleukin-1 receptor-associated kinase-1 degradation, i kappa b alpha phosphorylation/degradation, rela and ... | 2002 | 12140289 |
| structural study on lipid a and the o-specific polysaccharide of the lipopolysaccharide from a clinical isolate of bacteroides vulgatus from a patient with crohn's disease. | bacteroides vulgatus has been shown to be involved in the aggravation of colitis. previously, we separated two potent virulence factors, capsular polysaccharide (cps) and lipopolysaccharide (lps), from a clinical isolate of b. vulgatus and characterized the structure of cps. in this study, we elucidated the structures of o-antigen polysaccharide (ops) and lipid a in the lps. lps was subjected to weak acid hydrolysis to produce the lipid a fraction and polysaccharide fraction. lipid a was isolate ... | 2002 | 12153568 |
| quantitative analysis of bacterial dna from mycobacteria spp., bacteroides vulgatus, and escherichia coli in tissue samples from patients with inflammatory bowel diseases. | the etiology of inflammatory bowel diseases is unknown. mycobacteria spp., bacteroides vulgatus, and escherichia coli have been suspected to be involved. the aim of the present study was to examine the possible relationship between inflammatory bowel diseases and these microbes. | 2002 | 12162408 |
| design and evaluation of oligonucleotide-microarray method for the detection of human intestinal bacteria in fecal samples. | an oligonucleotide-microarray method was developed for the detection of intestinal bacteria in fecal samples collected from human subjects. the 16s rdna sequences of 20 predominant human intestinal bacterial species were used to design oligonucleotide probes. three 40-mer oligonucleotides specific for each bacterial species (total 60 probes) were synthesized and applied to glass slides. cyanine5 (cy5)-labeled 16s rdnas were amplified by polymerase chain reaction (pcr) from human fecal samples or ... | 2002 | 12167534 |
| national survey on the susceptibility of bacteroides fragilis group: report and analysis of trends for 1997-2000. | the results of a multicenter us survey using the national committee for clinical laboratory standards currently recommended methodology for measuring in vitro susceptibility of 2673 isolates of bacteroides fragilis group species were compared from 1997 to 2000. the test panel consisted of 14 antibiotics: 3 carbapenems, 3 beta-lactam-beta-lactamase inhibitors, 3 cephamycins, 2 fluoroquinolones, clindamycin, chloramphenicol, and metronidazole. declines in the geometric mean minimum inhibitory conc ... | 2002 | 12173121 |
| in vitro activities of newer quinolones against bacteroides group organisms. | the activities of bms-284576, clinafloxacin, moxifloxacin, sitafloxacin, trovafloxacin, imipenem, cefoxitin, and clindamycin against 589 bacteroides fragilis group isolates were determined. the activity of bms-284576 was comparable to that of trovafloxacin. sitafloxacin and clinafloxacin were the most active quinolones, and moxifloxacin was the least active. b. fragilis was the most susceptible of the species, and bacteroides vulgatus was the most resistant. association of specific antibiotic re ... | 2002 | 12234859 |
| innate immune responses of human neonatal cells to bacteria from the normal gastrointestinal flora. | the hygiene hypothesis postulates that the prevalence of allergy has increased due to decreased microbial stimulation early in life, leading to delayed maturation of the immune system. the aim of this study was to examine the cytokine pattern produced from cord blood mononuclear cells relative to adult cells after stimulation with bacterial strains from the normal flora. mononuclear cells from cord and adult blood samples were stimulated with the following bacteria: bifidobacterium adolescentis, ... | 2002 | 12438343 |
| quantification of bacteria adherent to gastrointestinal mucosa by real-time pcr. | the use of real-time quantitative pcr (5' nuclease pcr assay) as a tool to study the gastrointestinal microflora that adheres to the colonic mucosa was evaluated. we developed primers and probes based on the 16s ribosomal dna gene sequences for the detection of escherichia coli and bacteroides vulgatus. dna was isolated from pure cultures and from gut biopsy specimens and quantified by the 5' nuclease pcr assay. the assay showed a very high sensitivity: as little as 1 cfu of e. coli and 9 cfu of ... | 2002 | 12454130 |
| comparison of the faecal microflora of patients with ankylosing spondylitis and controls using molecular methods of analysis. | to determine whether differences within the complex intestinal microflora can be demonstrated between patients with ankylosing spondylitis (as) and healthy individuals. | 2002 | 12468819 |
| role of commensal bacteria in chronic experimental colitis: lessons from the hla-b27 transgenic rat. | rats on lewis or fischer background, transgenic for human hla-b27 and beta(2)-microglobulin genes spontaneously develop colitis, gastritis, arthritis, dermatitis, orchitis, epididymitis, carditis, alopecia and nail changes. disease susceptibility correlates with the gene copy number and is influenced by the genetic background. the pathomechanism in this model is still not completely understood. cell transfer experiments indicate an essential role of hla-b27 expression in bone marrow-derived cell ... | 2002 | 12571416 |
| lactobacillus gg prevents recurrence of colitis in hla-b27 transgenic rats after antibiotic treatment. | bacteroides vulgatus induces colitis in gnotobiotic hla-b27 transgenic (tg) rats while broad spectrum antibiotics prevent and treat colitis in specific pathogen free (spf) tg rats although disease recurs after treatment ends. lactobacilli treat human pouchitis and experimental colitis. we investigated if lactobacillus rhamnosus gg (l gg) can prevent colitis in tg rats monoassociated with b vulgatus and if l gg or lactobacillus plantarum 299v (lp 299v) can treat established colitis in spf tg rats ... | 2003 | 12584218 |
| activities of garenoxacin (bms-284756) and other agents against anaerobic clinical isolates. | a total of 590 clinical isolates consisting of 33 species of both gram-positive and gram-negative anaerobes were collected from nine centers in the chicago area in 1998-1999. the largest number of isolates (330 isolates, 56%) belonged to the bacteroides group. isolates were tested by agar dilution against garenoxacin (bms-284756, t-3811 me), trovafloxacin, moxifloxacin, clindamycin, imipenem, piperacillin-tazobactam, and cefoxitin. all but one species (2% of bacteroides vulgatus isolates) were f ... | 2003 | 12604521 |
| prevention of gut inflammation by bifidobacterium in dextran sulfate-treated gnotobiotic mice associated with bacteroides strains isolated from ulcerative colitis patients. | indigenous bacteroides strains are closely associated with the occurrence and exacerbation of ulcerative colitis (uc). in this study, we aimed to clarify the effect of bifidobacterium strains, another major member of colonic bacteria, on the development of gut inflammation using gnotobiotic mouse models associated with bacteroides strains isolated from uc patients. dextran sulfate (dss) administration induced inflammation in the large intestine, in particular of the cecum, in the gnotobiotic mic ... | 2003 | 12650769 |
| transforming growth factor-beta 1 inhibits non-pathogenic gram negative bacteria-induced nf-kappa b recruitment to the interleukin-6 gene promoter in intestinal epithelial cells through modulation of histone acetylation. | we have shown that non-pathogenic enteric gram-negative bacteroides vulgatus induces rela phosphorylation, nf-kappab activation, and proinflammatory gene expression in primary and intestinal epithelial cell (iec) lines. we now demonstrate the transient induction of nuclear phospho-rela (day 3) followed by persistent activation of phospho-smad2 (days 3 and 7) in iec from mucosal tissue sections of b. vulgatus-monoassociated rats, indicating that both nf-kappab and transforming growth factor-beta1 ... | 2003 | 12672795 |
| sequence analysis of cfxa2-like beta-lactamases in prevotella species. | sixty-two strains of oral (32) and non-oral (30) prevotella producing beta-lactamases were screened for cfxa by pcr, using an intragenic primer pair. all 62 were cfxa/cfxa2 positive. fourteen of these strains, representing seven pigmented and seven non-pigmented prevotella species were submitted to further pcr with specific primers that amplified the whole beta-lactamase structural gene (966 bp). after cloning and sequencing, the deduced amino acid sequences were compared with that of bacteroide ... | 2003 | 12697645 |
| fructans in the diet cause alterations of intestinal mucosal architecture, released mucins and mucosa-associated bifidobacteria in gnotobiotic rats. | the effects of fructans in the diet on the mucosal morphometry (height of villi, depth of the crypts, number of goblet cells), the thickness of the epithelial mucus layer and the histochemical composition of intestinal mucosubstances in the distal jejunum and the distal colon were investigated by comparing germ-free (gf) rats, rats harbouring bacteroides vulgatus and bifidobacterium longum (diassociated (da) rats), and rats with a human faecal flora (hfa). the rats were fed either a commercial s ... | 2003 | 12720580 |
| [intestinal flora and crohn's disease]. | the pathogenesis of inflammatory bowel diseases (ibd) proceeds through stages of initiation, amplification and healing. abundant clinical and experimental data incriminate luminal bacteria or bacterial products in both the initiation and perpetuation of chronic intestinal inflammation. macrophage and t-cell activation with accompanying inflammatory cytokine production appears to be an early event. studies of lymphocyte responsiveness to autologous and heterologous intestinal bacteria have sugges ... | 2003 | 12843962 |
| bacteroides vulgatus protects against escherichia coli-induced colitis in gnotobiotic interleukin-2-deficient mice. | the microflora plays a crucial role in inflammatory bowel diseases (ibds). specific pathogen-free (spf), but not germ-free, interleukin (il)-2-deficient (il-2-/-) mice develop colitis. the colitogenicity of commensal bacteria was determined. | 2003 | 12851881 |
| emergence of fluoroquinolone resistance among bacteroides species. | several newer generation fluoroquinolones have demonstrated good in vitro activity against bacteroides species; particularly when first introduced. however, resistance of bacteroides to quinolones appears to be increasing. | 2003 | 12865399 |
| accelerated alveolar bone loss in mice lacking interleukin-10. | interleukin-10 regulates pro-inflammatory cytokines, including those implicated in alveolar bone resorption. we hypothesized that lack of interleukin-10 leads to increased alveolar bone resorption. male interleukin-10(-/-) mice, on 129/svev and c57bl/6j background, were compared with age-, sex-, and strain-matched interleukin-10(+/+) controls for alveolar bone loss. immunoblotting was used for analysis of serum reactivity against bacteria associated with colitis and periodontitis. interleukin-10 ... | 2003 | 12885849 |
| the suppressive effect of bifidobacteria on bacteroides vulgatus, a putative pathogenic microbe in inflammatory bowel disease. | bacteroides, a predominant commensal bacteria in the gut, are thought to be responsible for the development of inflammatory bowel disease (ibd). in the present study, we examined whether or not bifidobacteria suppress b. vulgatus, a representative pathogenic bacteroides species, in both the coculture system and the gnotobiotic murine model. as a result, bifidobacterium infantis 1222 highly inhibited the growth of b. vulgatus in the coculture and also significantly suppressed the systemic antibod ... | 2003 | 12906096 |
| culture-independent identification of pathogenic bacteria and polymicrobial infections in the genitourinary tract of renal transplant recipients. | renal transplant recipients are predisposed to urinary tract infections caused by both common uropathogens and opportunistic bacteria resulting frequently in significant polymicrobial infections. in this study, a culture-independent 16s rrna-based approach was established to identify unusual, fastidious, or anaerobic bacteria and to investigate bacterial diversity in urinary tract specimens. similarly sized amplicons encompassing the v6 to v8 region of the 16s rrna were analyzed with denaturing ... | 2003 | 14662931 |
| genetic analysis of an ambler class a extended-spectrum beta-lactamase from capnocytophaga ochracea. | a beta-lactamase gene (cfxa3, 966 bp) was isolated from a beta-lactam-resistant capnocytophaga ochracea clinical isolate and amplified using primers from the cfxa gene of bacteroides vulgatus. the mics of third-generation cephalosporins were much higher than those of the transconjugant escherichia coli strain. the deduced protein sequence, by comparison with cfxa2 of prevotella intermedia, had a y239d substitution and possessed the characteristics of a class a, group 2e beta-lactamase. | 2004 | 14766881 |
| lipopolysaccharide induces cd25-positive, il-10-producing lymphocytes without secretion of proinflammatory cytokines in the human colon: low md-2 mrna expression in colonic macrophages. | despite the huge number of colonized gram-negative bacteria in the colon, the normal colon maintains its homeostasis without any excessive immune response. to investigate the potential mechanisms involved, human colonic lamina propria mononuclear cells (lpmcs) obtained from uninflamed mucosa were cultured with lipopolysaccharide (lps) prepared from bacteroides vulgatus (bv-lps) or bacteroides fragilis (bf-lps), as representatives of indigenous flora, or pathogenic salmonella minnesota (sm-lps). ... | 2004 | 14997033 |
| experimental swine dysentery: comparison between infection models. | the aim of the present study was to develop a reproducible porcine infection model with brachyspira hyodysenteriae. the influence of different factors was evaluated, namely, age, a diet containing large quantities of soybean meal, housing and administration of cortisol or antacids. furthermore, the synergistic effect of additional bacteria (escherichia coli o141, bacteroides vulgatus or a mixture of bacteroides fragilis, a field isolate of bacteroides and fusobacterium necrophorum) was studied. ... | 2004 | 15017282 |
| commensal gram-negative bacteria prime human dendritic cells for enhanced il-23 and il-27 expression and enhanced th1 development. | dendritic cells (dc) are the main orchestrators of specific immune responses. depending on microbial information they encounter in peripheral tissues, they promote the development of th1, th2 or unpolarized th cell responses. in this study we have investigated the immunomodulatory effect of non-pathogenic intestinal gram-negative (escherichia coli, bacteroides vulgatus,veillonella parvula, pseudomonas aeruginosa) and gram-positive (bifidobacterium adolescentis, enterococcus faecalis, lactobacill ... | 2004 | 15114670 |
| dna from probiotic bacteria modulates murine and human epithelial and immune function. | the intestinal epithelium must discriminate between pathogenic and nonpathogenic bacteria and respond accordingly. the aim of this study was to examine whether bacterial dna can serve as the molecular basis for bacterial recognition. | 2004 | 15131797 |
| differential effect of immune cells on non-pathogenic gram-negative bacteria-induced nuclear factor-kappab activation and pro-inflammatory gene expression in intestinal epithelial cells. | we have previously shown that non-pathogenic gram negative bacteria induce rela phosphorylation, nuclear factor (nf)-kappab transcriptional activity and pro-inflammatory gene expression in intestinal epithelial cells (iec) in vivo and in vitro. in this study, we investigated the molecular mechanism of immune-epithelial cell cross-talk on gram-negative enteric bacteria-induced nf-kappab signalling and pro-inflammatory gene expression in iec using ht-29/mtx as well as caco-2 transwell cultures int ... | 2004 | 15147574 |
| 15-deoxy-delta12,14-prostaglandin j2-mediated erk signaling inhibits gram-negative bacteria-induced rela phosphorylation and interleukin-6 gene expression in intestinal epithelial cells through modulation of protein phosphatase 2a activity. | we have previously shown that non-pathogenic gram-negative bacteroides vulgatus induces transient rela phosphorylation (ser-536), nf-kappab activity, and pro-inflammatory gene expression in native and intestinal epithelial cell (iec) lines. we now demonstrate that 15-deoxy-delta(12,14)-prostaglandin j(2) (15d-pgj(2)) but not prostaglandin e(2) inhibits lipopolysaccharide (lps) (b. vulgatus)/lps (escherichia coli)-induced rela phosphorylation and interleukin-6 gene expression in the colonic epith ... | 2004 | 15199053 |
| characterization of plasmid por1 from ornithobacterium rhinotracheale and construction of a shuttle plasmid. | the bacterium ornithobacterium rhinotracheale has been recognized as an emerging pathogen in poultry since about 10 years ago. knowledge of this bacterium and its mechanisms of virulence is still very limited. here we report the development of a transformation system that enables genetic modification of o. rhinotracheale. the system is based on a cryptic plasmid, por1, that was derived from an o. rhinotracheale strain of serotype k. sequencing indicated that the plasmid consisted of 14,787 nucle ... | 2004 | 15466524 |
| genetic and structural analysis of the bacteroides conjugative transposon ctn341. | the genetic structure and functional organization of a bacteroides conjugative transposon (ctn), ctn341, were determined. ctn341 was originally isolated from a tetracycline-resistant clinical isolate of bacteroides vulgatus. the element was 51,993 bp long, which included a 5-bp coupling sequence that linked the transposon ends in the circular form. there were 46 genes, and the corresponding gene products fell into three major functional groups: dna metabolism, regulation and antibiotic resistanc ... | 2005 | 15805532 |
| bacteroides massiliensis sp. nov., isolated from blood culture of a newborn. | gram-negative anaerobic rods were isolated from blood culture of a healthy baby born at term. based on phylogenetic analysis and specific phenotypic characteristics, this strain was included within the bacteroides cluster. the novel bile-resistant anaerobic species was designated bacteroides massiliensis and it has 92.8 % 16s rrna similarity to bacteroides vulgatus and a dna g + c content of 49 mol%. the major cellular fatty acid is anteiso-branched c(15 : 0). the type strain of b. massiliensis ... | 2005 | 15879278 |
| association with selected bacteria does not cause enterocolitis in il-10 gene-deficient mice despite a systemic immune response. | resident bacteria have been implicated to play a major role in the development of inflammatory bowel disease. while luminally sterile il-10 gene-deficient mice remain disease-free, their conventionally raised littermates develop enterocolitis associated with increased numbers of luminal and mucosal adherent bacteria. to investigate the role of defined bacteria on the initiation and development of this enterocolitis, we associated luminally sterile il-10 gene-deficient mice with pure strains of r ... | 2005 | 15906767 |
| in vitro activity of tigecycline against bacteroides species. | to ascertain the current susceptibility patterns of members of the bacteroides fragilis group in our hospital and to assess the in vitro activity of tigecycline against these organisms. | 2005 | 15951353 |
| innate mechanisms for bifidobacterium lactis to activate transient pro-inflammatory host responses in intestinal epithelial cells after the colonization of germ-free rats. | bifidobacteria comprise a dominant microbial population group in the human intestinal tract with purported beneficial health effects on the host. in this study, we characterized the molecular mechanisms for the initial interaction of probiotic bifidobacterium lactis strain bb12 with native and intestinal epithelial cell (iec) lines. we showed that b. lactis-monoassociated fisher f344 rats transiently induce phosphorylation/activation of the nf-kappab transcriptionally active subunit rela and the ... | 2005 | 16011513 |
| trypanosoma cruzi: influence of predominant bacteria from indigenous digestive microbiota on experimental infection in mice. | to verify the influence of some predominant components from indigenous microbiota on systemic immunological responses during experimental chagas disease, germ-free nih swiss mice were mono-associated with escherichia coli, enterococcus faecalis, bacteroides vulgatus or peptostreptococcus sp. and then infected with the y strain of trypanosoma cruzi. all the mono-associations predominantly induced a th1 type of specific immune response to the infection by t. cruzi. a direct correlation was observe ... | 2005 | 16038901 |
| bacteroides plebeius sp. nov. and bacteroides coprocola sp. nov., isolated from human faeces. | nine strains of gram-negative, anaerobic rod were isolated from human faeces. based on phylogenetic analysis and specific phenotypic characteristics, these strains were included within the bacteroides cluster and were divided into two clusters. strains from the two clusters showed 16s rrna gene sequence similarities of 90.4 and 92.7% to the nearest recognized species, bacteroides vulgatus. the strains also formed two clusters exhibiting a 16s rrna gene sequence divergence of approximately 6%. dn ... | 2005 | 16166722 |
| effects of difructose anhydride iii (dfa iii) administration on rat intestinal microbiota. | the effects of difructose anhydride iii (di-d-fructofuranose-1,2':2,3'-dianhydride; dfa iii) administration (3% dfa iii for 4 weeks) on rat intestinal microbiota were examined using denaturing gradient gel electrophoresis (dgge). according to dgge profiles, the number of bacteria related to bacteroides acidofaciens and uncultured bacteria within the clostridium lituseburense group decreased, while that of bacteria related to bacteroides vulgatus, bacteroides uniformis and ruminococcus productus ... | 2005 | 16233782 |
| bacterial-induced inflammation in germ-free rabbit appendix. | the intestinal ecosystem is defined by a series of interactions between the microbiota, the mucosal epithelium, and the gut-associated lymphoid tissue (galt). perturbations in the fine balance of the interactions between these components can result in gastrointestinal diseases such as inflammatory bowel disease (ibd). the pathophysiology of ibd is thought to develop as a result of dysregulated mucosal immune responses to normal luminal microflora. several animal models for ibd have been develope ... | 2005 | 16239845 |
| gut-associated bacterial microbiota in paediatric patients with inflammatory bowel disease. | clinical and experimental observations in animal models indicate that intestinal commensal bacteria are involved in the initiation and amplification of inflammatory bowel disease (ibd). no paediatric reports are available on intestinal endogenous microflora in ibd. | 2006 | 16648155 |
| colitogenic and non-colitogenic commensal bacteria differentially trigger dc maturation and th cell polarization: an important role for il-6. | we investigated whether commensal bacteria modulate activation and maturation of bone marrow-derived dc and their ability to prime cd4(+) t cells. we used escherichia coli mpk, which induces colitis in gnotobiotic il-2-deficient (il-2(-/-)) mice, and bacteroides vulgatus mpk, which prevents e. coli-induced colitis. stimulation of dc with e. coli induced tnf-alpha, il-12 and il-6 secretion and expression of activation markers. moreover, stimulation of dc with e. coli increased t cell activation a ... | 2006 | 16708404 |
| persistence and growth of fecal bacteroidales assessed by bromodeoxyuridine immunocapture. | extraintestinal growth of fecal bacteria can impair accurate assessment of watershed health. anaerobic fecal bacteria belonging to the order bacteroidales are attractive candidates for fecal source tracking because they have host-specific distributions and do not grow well in the presence of high oxygen concentrations. growth of general and human-specific fecal bacteroidales marker organisms in environmental samples (sewage) and persistence of the corresponding genetic markers were investigated ... | 2006 | 16820440 |
| bacteroides dorei sp. nov., isolated from human faeces. | two gram-negative, anaerobic, non-spore-forming rod-shaped organisms were isolated from human faeces. these isolates were tentatively identified as bacteroides based on morphological and biochemical criteria and appeared closely related to bacteroides vulgatus atcc 8482(t). the 16s rrna gene sequence analysis showed that the isolates were highly related to each other (99.5 %) and confirmed their placement in the genus bacteroides. 16s rrna gene sequence similarity values with close phylogenetic ... | 2006 | 16825642 |
| deoxycholic acid formation in gnotobiotic mice associated with human intestinal bacteria. | in humans and animals, intestinal flora is indispensable for bile acid transformation. the goal of our study was to establish gnotobiotic mice with intestinal bacteria of human origin in order to examine the role of intestinal bacteria in the transformation of bile acids in vivo using the technique of gnotobiology. eight strains of bile acid-deconjugating bacteria were isolated from ex-germ-free mice inoculated with a human fecal dilution of 10(-6), and five strains of 7alpha-dehydroxylating bac ... | 2006 | 17152920 |
| cd4(+) t lymphocytes mediate colitis in hla-b27 transgenic rats monoassociated with nonpathogenic bacteroides vulgatus. | hla-b27/beta2 microglobulin transgenic (tg) rats develop spontaneous colitis when raised under specific pathogen-free (spf) conditions or after mono-association with bacteroides vulgatus (b. vulgatus), whereas germ-free tg rats fail to develop intestinal inflammation. spf hla-b27 tg rnu/rnu rats, which are congenitally athymic, remain disease free. these results indicate that commensal intestinal bacteria and t cells are both pivotal for the development of colitis in tg rats. however, it is not ... | 2007 | 17206701 |
| therapeutic effects of roxithromycin in interleukin-10-deficient colitis. | a limited number of therapeutic strategies are currently available to treat patients with inflammatory bowel disease (ibd). interleukin-10 (il-10)-deficient mice, well characterized as an experimental model of ibd, develop severe chronic colitis because of aberrant th1 responses. roxithromycin (rxm), a macrolide antibiotic, has received attention because it offers not only antibacterial but also immunosuppressive effects. we examined the immunosuppressive effect of rxm on the development of ibd. | 2007 | 17245768 |
| epithelial barrier disruption allows nondisease-causing bacteria to initiate and sustain ibd in the il-10 gene-deficient mouse. | in the il-10 gene-deficient mouse model, development of intestinal inflammation is associated with a defect in epithelial barrier integrity that is thought to allow sufficient passage of bacteria or bacterial antigens to initiate a mucosal immune response. microbial monoassociation experiments into axenic animals have shown that some, but not all, endogenous bacteria will initiate an intestinal inflammatory response. for instance, bacteroides vulgatus does not initiate intestinal inflammation in ... | 2007 | 17427241 |
| reduced mucosal antimicrobial activity in crohn's disease of the colon. | in order to maintain the mucosal barrier against luminal microorganisms the intestinal epithelial cells synthesise various broad-spectrum antimicrobial peptides including defensins and cathelicidins. recent studies indicate that both may be deficient in crohn's disease. to elucidate the possible functional consequences of this deficiency antimicrobial activity in colonic mucosa from patients with inflammatory bowel disease and healthy controls was investigated. | 2007 | 17456510 |
| identification of commensal bacterial strains that modulate yersinia enterocolitica and dextran sodium sulfate-induced inflammatory responses: implications for the development of probiotics. | an increasing body of evidence suggests that probiotic bacteria are effective in the treatment of enteric infections, although the molecular basis of this activity remains elusive. to identify putative probiotics, we tested commensal bacteria in terms of their toxicity, invasiveness, inhibition of yersinia-induced inflammation in vitro and in vivo, and modulation of dextran sodium sulfate (dss)-induced colitis in mice. the commensal bacteria escherichia coli, bifidobacterium adolescentis, bacter ... | 2007 | 17485456 |
| evolution of symbiotic bacteria in the distal human intestine. | the adult human intestine contains trillions of bacteria, representing hundreds of species and thousands of subspecies. little is known about the selective pressures that have shaped and are shaping this community's component species, which are dominated by members of the bacteroidetes and firmicutes divisions. to examine how the intestinal environment affects microbial genome evolution, we have sequenced the genomes of two members of the normal distal human gut microbiota, bacteroides vulgatus ... | 2007 | 17579514 |
| bacteroides sp. strain d8, the first cholesterol-reducing bacterium isolated from human feces. | the microbial community in the human colon contains bacteria that reduce cholesterol to coprostanol, but the species responsible for this conversion are still unknown. we describe here the first isolation and characterization of a cholesterol-reducing bacterium of human intestinal origin. strain d8 was isolated from a 10(-8) dilution of a fresh stool sample provided by a senior male volunteer with a high capacity to reduce luminal cholesterol to coprostanol. cholesterol-to-coprostanol conversion ... | 2007 | 17616613 |
| dual-association of gnotobiotic il-10-/- mice with 2 nonpathogenic commensal bacteria induces aggressive pancolitis. | monoassociating gnotobiotic il-10-deficient (-/-) mice with either nonpathogenic enterococcus faecalis or a nonpathogenic escherichia coli strain induces t-cell-mediated colitis with different kinetics and anatomical location (e. faecalis: late onset, distal colonic; e. coli: early onset, cecal). hypothesis: e. faecalis and e. coli act in an additive manner to induce more aggressive colitis than disease induced by each bacterial species independently. | 2007 | 17763473 |
| gut microbes in crohn's disease: getting to know you better? | biomarkers in inflammatory bowel disease (ibd) are needed to help in disease diagnosis, prognosis, and assessment of disease activity. the best serologic markers to date measure antibody responses to normal commensal flora. these antibody responses can provide prognostic information as well as clues to disease pathogenesis. there remains room for additional biomarkers to clarify the diagnosis for certain patients with indeterminate colitis or small bowel crohn's disease (cd) with protean manifes ... | 2008 | 18289202 |
| effects of rosa rugosa petals on intestinal bacteria. | the effects of pulverized petal of rosa rugosa on the growth of 10 species of intestinal and pathogenic bacteria were investigated. growth of bifidobacteria and lactobacilli was not affected by the addition of the petal in plate cultivation. however, the growth of bacteroides vulgatus, escherichia coli, staphylococcus aureus, and bacillus cereus was completely inhibited by the addition of 0.1, 0.5, 0.1, and 0.05% (w/v) of the petal respectively. in liquid cultivation, the addition of the petal ( ... | 2008 | 18323655 |
| detection of possible ai-2-mediated quorum sensing system in commensal intestinal bacteria. | the vibrio harveyi strain bb170-autoinducer bioassay was used to detect possible quorum sensing autoinducer-2 molecule (ai-2) in culture fluids of commensal intestinal bacteria. culture fluids of bacteroides vulgatus, clostridium proteoclasticum, escherichia coli, eubacterium rectale, lachnospira multipara, pseudobutyrivibrio ruminis, roseburia intestinalis, ruminococcus albus and ruminococcus flavefaciens contained ai-2-like molecules. the pcr bands from some of the tested strains could be also ... | 2008 | 18661296 |
| detection and persistence of fecal bacteroidales as water quality indicators in unchlorinated drinking water. | the results of this study support the use of fecal bacteroidales qpcr as a rapid method to complement traditional, culture-dependent, water quality indicators in systems where drinking water is supplied without chlorination or other forms of disinfection. a sybr-green based, quantitative pcr assay was developed to determine the concentration of fecal bacteroidales 16s rrna gene copies. the persistence of a bacteroides vulgatus pure culture and fecal bacteroidales from a wastewater inoculum was d ... | 2009 | 19261420 |
| bioactivation of selenocysteine derivatives by beta-lyases present in common gastrointestinal bacterial species. | studies in cell cultures and animal models have demonstrated cancer chemopreventive effects of certain selenium compounds. here we describe the screening of cysteine s-conjugate beta-lyase activity in bacterial species that are implicated in the bio-activation of sulfur- and selenocysteine derivatives. we screened a range of bacterial species commonly found in the human intestine for beta-lyase activity on se-p-methoxybenzylselenocysteine and the natural occurring s-methylcysteine and se-methyls ... | 2008 | 19326339 |
| commensal bacteria can enter colonic epithelial cells and induce proinflammatory cytokine secretion: a possible pathogenic mechanism of ulcerative colitis. | interleukin 2 (il-2)- and il-10-knockout mice develop spontaneous colitis under conventional but not germ-free conditions, suggesting that commensal bacteria play an important role in the pathogenesis of colitis. however, interactions between commensal bacteria and colonic epithelial cells have not been fully investigated. we therefore assessed the ability of various commensal bacteria and probiotics to adhere to and invade colonic epithelial cells. effects of the bacteria on production of proin ... | 2009 | 19369513 |
| pattern extraction of structural responses of gut microbiota to rotavirus infection via multivariate statistical analysis of clone library data. | this study developed a new statistical strategy for analyzing clone library data to observe whether there is a defined pattern in structural responses of gut microbiota to environmental perturbations. a large clone library of genus bacteroides was constructed with fecal samples for each subject in rotavirus-infected (group r) and healthy children (group h). in all, 665 clones of the 12 group h subjects and 284 clones of the nine group r subjects were sequenced and classified into 34 operational ... | 2009 | 19453743 |
| modulation of the intestinal microbiota alters colitis-associated colorectal cancer susceptibility. | it is well established that the intestinal microbiota plays a key role in the pathogenesis of crohn's disease (cd) and ulcerative colitis (uc) collectively referred to as inflammatory bowel disease (ibd). epidemiological studies have provided strong evidence that ibd patients bear increased risk for the development of colorectal cancer (crc). however, the impact of the microbiota on the development of colitis-associated cancer (cac) remains largely unknown. in this study, we established a new mo ... | 2009 | 19551144 |
| molecular inventory of faecal microflora in patients with crohn's disease. | intestinal microbial community is involved in the pathogenesis of crohn's disease, but knowledge of its potential abnormalities has been limited by the impossibility to grow many dominant intestinal bacteria. using sequence analysis of randomly cloned bacterial 16s ribosomal dna, the dominant faecal species from four crohn's disease patients and four controls were compared. whereas marked inter-individual differences were observed in the faecal microflora of patients, three remained distantly re ... | 2004 | 19712374 |
| transepithelial activation of human leukocytes by probiotics and commensal bacteria: role of enterobacteriaceae-type endotoxin. | the goal of the current study was to clarify whether commercially available probiotics induce greater trans-epithelial activation of human leukocytes than do commensal, food-derived and pathogenic bacteria and to identify the compounds responsible for this activation. eleven different bacterial strains, and some of their pathogen-associated molecular patterns, were incubated apically on a confluent layer of intestinal epithelial cells (caco-2), which were basolaterally co-cultured with human mon ... | 2009 | 19714861 |
| lessons learned from the anaerobe survey: historical perspective and review of the most recent data (2005-2007). | the rationale and lessons learned through the evolution of the national survey for the susceptibility of bacteroides fragilis group from its initiation in 1981 through 2007 are reviewed here. the survey was conceived in 1980 to track emerging antimicrobial resistance in bacteroides species. | 2010 | 20067390 |
| molecular characterisation of the faecal microbiota in patients with type ii diabetes. | the investigation provides molecular analyses of the faecal microbiota in type 2 diabetic patients. in order to characterise the gut microbiota in diabetic patients and to assess whether there are changes in the diversity and similarity of gut microbiota in diabetic patients when compared with healthy individuals, bacterial dnas from 16 type 2 diabetic patients and 12 healthy individuals were extracted from faecal samples and characterised by pcr-denaturing gradient gel electrophoresis (dgge) wi ... | 2010 | 20087741 |
| identification of clinically important anaerobic bacteria by an oligonucleotide array. | anaerobic bacteria can cause a wide variety of infections, and some of these infections can be serious. conventional identification methods based on biochemical tests are often lengthy and can produce inconclusive results. an oligonucleotide array based on the 16s-23s rrna intergenic spacer (its) sequences was developed to identify 28 species of anaerobic bacteria and veillonella. the method consisted of pcr amplification of the its regions with universal primers, followed by hybridization of th ... | 2010 | 20129959 |
| bacteroides paurosaccharolyticus sp. nov., isolated from a methanogenic reactor treating waste from cattle farms. | a strictly anaerobic bacterial strain (wk042(t)) was isolated from rice-straw residue in a methanogenic reactor treating waste from cattle farms in japan. cells were gram-staining-negative, non-motile, non-spore-forming rods. growth was stimulated well by haemin, and was enhanced by cobalamin (vitamin b(12)). strain wk042(t) utilized arabinose, xylose, glucose, mannose and aesculin as preferred substrates. maltose, dextrin, glycogen, starch and pectin were also utilized, although growth on these ... | 2011 | 20348319 |
| isolation of bacteria from mouse caecal samples and description of bacteroides sartorii sp. nov. | caecal samples from wild-type and tnf(deltaare) mice were cultured on selective media containing bile salts, amino acids or casein macro-peptides. twenty-two strains were isolated and identified by 16s rrna gene sequencing. twenty-one strains showed >98% similarity to known bacteria (blautia spp., clostridium innocuum, enterococcus spp., escherichia coli, lactobacillus murinus, parabacteroides goldsteinii and shigella dysenteriae). one additional isolate, strain a-c2-0, was a new bacterium. the ... | 2010 | 20369227 |
| consumption of human milk oligosaccharides by gut-related microbes. | human milk contains large amounts of complex oligosaccharides that putatively modulate the intestinal microbiota of breast-fed infants by acting as decoy binding sites for pathogens and as prebiotics for enrichment of beneficial bacteria. several bifidobacterial species have been shown to grow well on human milk oligosaccharides. however, few data exist on other bacterial species. this work examined 16 bacterial strains belonging to 10 different genera for growth on human milk oligosaccharides. ... | 2010 | 20394371 |
| safety of probiotic escherichia coli strain nissle 1917 depends on intestinal microbiota and adaptive immunity of the host. | probiotics are viable microorganisms that are increasingly used for treatment of a variety of diseases. occasionally, however, probiotics may have adverse clinical effects, including septicemia. here we examined the role of the intestinal microbiota and the adaptive immune system in preventing translocation of probiotics (e.g., escherichia coli nissle). we challenged c57bl/6j mice raised under germfree conditions (gf-raised c57bl/6j mice) and rag1(-/-) mice raised under germfree conditions (gf-r ... | 2010 | 20421387 |
| ompa variants affecting the adherence of ulcerative colitis-derived bacteroides vulgatus. | to investigate the adhesion factor of bacteroides vulgatus derived from ulcerative colitis (uc), we isolated b. vulgatus strains from the large intestinal mucosa of uc patients and non-uc individuals, and examined their adherence to tissue-cultured cells. the adherence to tissue-cultured cells in uc-derived strains (36.5 +/- 7.9 %) was higher than that in non-uc-derived strains (13.2 +/- 7.7 %). pcr and sequencing analysis of outer membrane proteins revealed that the strains derived from five of ... | 2010 | 20437766 |
| bacteroides chinchillae sp. nov. and bacteroides rodentium sp. nov., isolated from chinchilla (chinchilla lanigera) faeces. | gram-negative anaerobic rods were isolated from chinchilla (chinchilla lanigera) faeces and three strains, st170(t), st180 and st28(t), were investigated taxonomically. on the basis of phylogenetic analyses and specific phenotypic characteristics, the three strains belonged to the genus bacteroides. phylogenetic analysis of their 16s rrna gene sequences revealed that strains st170(t) and st180 formed a single cluster and a distinct line of descent. strain st170(t) exhibited 99.7 % 16s rrna gene ... | 2011 | 20495039 |
| a distinctive 'microbial signature' in celiac pediatric patients. | celiac disease (cd) is an autoimmune disorder of the small intestine in which dietary gluten ingestion leads to a chronic enteropathy. recently, scientific evidence suggested a potential role of gut microbiota in cd. to have a snapshot of dominant duodenal microbiota we analyzed the mucosa-associated microbiota of 20 children with cd, before and after a gluten-free diet (gfd) regimen, and of 10 controls. total dna was extracted from duodenal biopsies and amplification products of 16s ribosomal d ... | 2010 | 20565734 |