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failure to isolate yersinia enterocolitica from patients with diarrhoea in singapore.to determine the presence of yersinia enterocolitica in singapore, an attempt was made to isolate this organism from the stool samples of 6,619 patients with diarrhoea. no y. enterocolitica was detected. the results suggest that y. enterocolitica is not an important bacterial pathogen causing diarrhoeal disease in this region.19921430971
effect of ethylenediamine di-o-hydroxyphenylacetic acid and transferrin on the growth of some bacterial strains in vitro.the ability of some bacterial strains to obtain iron from ethylenediamine di-o-hydroxyphenylacetic acid (edda) or iron-free transferrin, and accordingly grow in their presence, was studied. growth of staphylococcus aureus and yersinia pseudotuberculosis was inhibited by edda or by iron-free transferrin. growth of streptococcus faecalis, however, was inhibited by iron-free transferrin, but not by edda. the other bacterial strains, i.e.; escherichia coli, salmonella typhimurium and shigella dysent ...19921435350
beta-lactamase induction by imipenem in yersinia enterocolitica.five strains of yersinia enterocolitica belonging to 5 different biotypes were induced to beta-lactamase production with imipenem in broth culture at 28 degrees c. strains belonging to biotypes 1a, 1b, 2 and 3 showed a 26 to 73 fold increase in beta-lactamase activity after 180 min in the presence of 0.5 mg/l imipenem. biotype 4 strain, by contrast, did not show obvious enhancement of its beta-lactamase activity in the same experiments. enzyme b in biotypes 1b, 2 and 3 and enzyme "b-like" in bio ...19921437295
a surface protease and the invasive character of plague.a 9.5-kilobase plasmid of yersinia pestis, the causative agent of plague, is required for high virulence when mice are inoculated with the bacterium by subcutaneous injection. inactivation of the plasmid gene pla, which encodes a surface protease, increased the median lethal dose of the bacteria for mice by a millionfold. moreover, cloned pla was sufficient to restore segregants lacking the entire pla-bearing plasmid to full virulence. both pla+ strains injected subcutaneously and pla- mutants i ...19921439793
evaluation of data transformations used with the square root and schoolfield models for predicting bacterial growth rate.a comparison was made between mathematical variations of the square root and schoolfield models for predicting growth rate as a function of temperature. the statistical consequences of square root and natural logarithm transformations of growth rate use in several variations of the schoolfield and square root models were examined. growth rate variances of yersinia enterocolitica in brain heart infusion broth increased as a function of temperature. the ability of the two data transformations to c ...19921444367
incidence and coincidence of listeria spp., motile aeromonads and yersinia enterocolitica on ready-to-eat fleshfoods.a survey for the presence of listeria spp., yersinia enterocolitica and motile aeromonads in 203 samples of ready-to-eat fleshfoods purchased from retail outlets was conducted. overall, 39.4%, 3.4% and 23.2% of samples were positive for the presence of listeria spp., y. enterocolitica and motile aeromonads respectively. two factors have been identified as contributing to contamination of fleshfoods by these cold-tolerant bacteria. these are (i) the method of sale; delicatessen-bought foods were ...19921445763
removal of yersinia enterocolitica from fresh parsley by washing with acetic acid or vinegar.different washing procedures and solutions were applied to fresh parsley and the effect on artificially contaminated y. enterocolitica and the natural population of aerobic bacteria was determined. dipping the parsley containing 10(7) y. enterocolitica per gram into the 2% (v/v) acetic acid or 40% (v/v) vinegar solutions for 15 min exerted pronounced bactericidal effect against this organism. no viable aerobic bacteria were recovered after 30 min dip in 5% (v/v) acetic acid, whereas vinegar led ...19921445772
[role of histamine receptors of mouse and guinea pig peritoneal leukocytes in the pathogenetic effect of yersinia pestis adenylate cyclase].the effect of purified preparation of adenylate cyclase isolated from y. pestis on peritoneal leukocytes of white mice and guinea pigs was investigated. y. pestis adenylate cyclase was shown to accomplish its pathogenic action via histamine-specific receptors on the surface of eukaryotic cells. the involvement of h1 and h2 histamine receptors on target cells in the adenylate cyclase action leading to development of plague infection is discussed.19921446035
[the successful treatment of an acute form of crohn's disease]. 19921447894
loss of the pigmentation phenotype in yersinia pestis is due to the spontaneous deletion of 102 kb of chromosomal dna which is flanked by a repetitive element.the pigmentation (pgm+) phenotype of yersinia pestis encompasses a variety of different physiological traits, all of which are missing in pgm- mutants. we have previously shown that loss of the pgm+ phenotype is accompanied by the spontaneous deletion of at least 45 kb of chromosomal dna, referred to as the pgm locus. using chromosomal walking, we have now mapped the full extent of the pgm locus in y. pestis strain kim6+. our results indicate that the locus spans 102 kb of dna which is absent in ...19921447977
[yersinia-induced arthritis in patients with spondylitis ankylopoietica of 20-year duration].the case of a 46 years old woman with spondylitis ankylopoetica is reported. following diarrhoea a severe, asymmetric oligoarthritis developed with positive yersinia enterocolitica serology. the acute oligoarthritis repressed after 2 month.19921448276
a rapid and sensitive method for the identification of brucella species with a monoclonal antibody.a coagglutination test using monoclonal antibody bme10-5 with specificity for the m antigen of brucella melitensis 16m has been developed for the rapid identification of the smooth brucella species. all reference strains of several biovars of b. melitensis, b. abortus, b. suis and b. neotomae tested were positive in this assay. no significant differences in reaction intensity were observed in relation to the different distribution of the a and m antigens among the brucella serovars analysed. con ...19921448627
yersinia pestis yopm: thrombin binding and overexpression.in previous studies, yersinia pestis yopm has been shown through mutational analysis to be necessary for virulence in mice and found to have homology with the thrombin-binding domain of the platelet receptor gpib alpha. in this study, yopm was purified and shown by dot blot and chemical cross-linking tests to bind to human alpha-thrombin. no cross-linked product could be detected when human prothrombin was incubated with yopm. as a functional test of thrombin binding, it was shown that native bu ...19921452357
[a new r-plasmid from yersinia pseudotuberculosis].yersinia pseudotuberculosis strain 140-p isolated from soil in the far east was found to harbour an r-plasmid different from the plasmids that had been isolated from the bacteria previously. a new r-plasmid pld140 is conjugation proficient and codes for the cellular resistance to streptomycin, tetracycline and sulfonamides. the plasid belongs to incompatibility group incp. its restriction endonucleases bamhi and sali profile is different from the ones of the plasmids belonging to the rp4 family.19921454082
the regulation of brush border surface area. 19921456665
anti-enterobacteria antibodies in psoriatic arthritis.the occurrence of certain antibacterial antibodies was studied in the sera of 22 healthy donors (hd) and 66 patients with different diseases. the cases investigated included 22 rheumatoid arthritis (ra), 22 non-arthritic-psoriasis (nap), and 22 psoriatic arthritis (pa) patients. a complement fixation test was used with yersinia enterocolitica 0:3 type (yec), yersinia pseudotuberculosis (ypt), campylobacter jejuni (cj), and campylobacter fetus (cf) antigens; the detection of anti-chlamydia tracho ...19921458698
synergistic growth studies of entamoeba gingivalis using an ecologen.a unique multiple diffusion growth chamber, an ecologen, designed for the study of interactions among microorganisms, was introduced as a means of growing xenic cultures of entamoeba gingivalis with crithidia sp. or yersinia enterocolitica. entamoeba gingivalis was grown in the central diffusion reservoir of the ecologen connected to separate growth chambers inoculated with the microorganisms to be evaluated. growth of the accompanying bacteria in the e. gingivalis compartment was almost complet ...19921459786
identification of surface-exposed yersinia pestis proteins by radio-iodination and biotinylation.when whole cells (stationary phase) of yersinia pestis strain ev76 were radiolabelled with iodogen and 131i, 16 major and 10 minor surface-exposed outer membrane proteins (omps) were identified. labelling with n-hydroxysuccinimidyl 6-biotinylamino-hexanoate (biotin x-nhs) resulted in a complex protein profile detectable after blotting and developing with peroxidase-conjugated avidin. y. pestis cell fractionation revealed that biotin x-nhs labelled not only omps but also proteins of inner cell co ...19921460662
[diagnosis of acute diarrheal infections]. 19921460834
survival and causes of death among patients with yersinia enterocolitica infection. a norwegian 10-year follow-up study on 458 hospitalized patients.the aim of the present study was to elucidate the possible influence of the yersinia enterocolitica infection on long-time survival, and to describe clinical conditions associated with a fatal issue. during the period 1974-83, y. enterocolitica infection was diagnosed in 458 hospitalized patients by antibody response or isolation of the microorganism. the patients were followed for 4-14 years (until 1987). the observed cumulative survival rates for female patients, and for the whole material, de ...19921465579
[bacterial pathogens in diarrhea: demonstration of verotoxin-producing escherichia coli using the polymerase chain reaction].in 23% of 576 diarrhea patients we were able to demonstrate putative bacterial pathogens. not included in this number is clostridium difficile which was found in 8 of 48 (8.3%) specimens analyzed. in addition to salmonella (8.2%), campylobacter (6.0%), aeromonas (2.7%), shigella (1.9%) and yersinia (0.8%) we identified verotoxin-producing escherichia coli (vtec) in 10 patients (1.9%). vtec were found as frequently as shigella (occurring exclusively in patients returning from the tropics) and we ...19921465595
aetiological role of bacteria associated with reactive arthritis in pauciarticular juvenile chronic arthritis.the cause of juvenile chronic arthritis (jca) is unknown. pauciarticular jca is the most common subtype and can be subdivided into early (type i) and late onset (type ii) forms, the latter clinically resembling reactive arthritis.19921466598
antibody response against 26 and 46 kilodalton released proteins of yersinia in yersinia triggered reactive arthritis.using an inhibition enzyme linked immunosorbent assay (elisa) the serum antibody responses against released proteins of yersinia of molecular weights 26 and 46 kilodaltons were studied in 37 patients with and in 21 patients without reactive arthritis following yersinia infection. although no difference was seen for the 46 kilodalton released protein, patients with yersinia triggered reactive arthritis had higher levels of antibodies against the 26 kilodalton released protein at the beginning of ...19921466603
transfusing yersinia enterocolitica. 19921467705
destruction of bacteria on fresh meat by hot water.strains of different bacterial genera; escherichia coli (7), salmonella serotypes (7), enteropathogenic e. coli o 157 (2), aeromonas hydrophila (4), yersinia enterocolytica (1), pseudomonas fragi (5), and listeria monocytogenes (5), inoculated onto the surface tissues of pieces of fresh beef were found to be susceptible to the lethal action of hot water. on average, more than 3 log10 (99.9%) of these organisms were destroyed when water at 80 degrees c was applied for 10 or 20 s. therefore, a hot ...19921468533
chronic intussusception associated with yersinia enterocolitica mesenteric adenitis.an unusual case of chronic intussusception, without any digestive sign, secondary to mesenteric lymphadenitis caused by yersinia enterocolitica is reported. operative reduction by taxis was performed but ileopexy and antibiotic treatment were also carried out to reduce chances of recurrent intussusception.19921469587
direct identification of yersinia enterocolitica in blood by polymerase chain reaction amplification.primers based on the nucleotide sequence of the virf gene in the pyv plasmid and the chromosomal ail gene were used in polymerase chain reaction (pcr) amplifications to directly identify yersinia enterocolitica in blood. approximately 500 bacteria seeded into 100 microl of blood can be extracted and amplified by pcr to yield positive results. pcr analyses of seven y. enterocolitica isolates previously implicated in blood contaminations showed that only one isolate harbored the plasmid-borne virf ...19921471250
hrp genes of pseudomonas solanacearum are homologous to pathogenicity determinants of animal pathogenic bacteria and are conserved among plant pathogenic bacteria.the majority of bacterial plant diseases are caused by members of three bacterial genera, pseudomonas, xanthomonas, and erwinia. the identification and characterization of mutants that have lost the abilities to provoke disease symptoms on a compatible host and to induce a defensive hypersensitive reaction (hr) on an incompatible host have led to the discovery of clusters of hrp genes (hypersensitive reaction and pathogenicity) in phytopathogenic bacteria from each of these genera. here, we repo ...19921472716
determinants of pathogenicity in xanthomonas campestris pv. vesicatoria are related to proteins involved in secretion in bacterial pathogens of animals.one of the model systems investigated for studying plant bacterial pathogenesis is xanthomonas campestris pv vesicatoria, the causal agent of bacterial spot disease of pepper and tomato. genes necessary for both basic pathogenicity and the induction of the hypersensitive response in resistant plants (hrp genes) were previously isolated from x. c. pv. vesicatoria and characterized genetically. as a first step toward functional analysis, part of the hrp gene cluster, making up several loci, was se ...19921472717
plague surveillance by serological testing of coyotes (canis latrans) in los angeles county, california.sera from 19 (5.6%) of 338 coyotes (canis latrans) collected in los angeles county, california between 1986 and 1990 had antibodies against yersinia pestis using the passive hemagglutination test. coyotes were excellent sentinels of plague activity in los angeles county. enzootic plague declined dramatically in the county during the 5-yr period.19921474660
the interaction of bacteria with mammalian cells. 19921476803
hla-b27/microbial mimicry: an in vivo analysis.the association between three major spondyloarthritic diseases, ankylosing spondylitis, reiter's syndrome, and reactive arthritis, and the major histocompatibility complex (mhc) class 1 antigen hla-b27 is well documented. the hypothesis of cross-reactivity between hla-b27 and the antecedent infection-causing gram-negative pathogens such as salmonella, shigella and yersinia has been suggested by in vitro studies employing monoclonal antibodies. we have examined the possibility of such cross-react ...19921478690
evidence that the hrpb gene encodes a positive regulator of pathogenicity genes from pseudomonas solanacearum.the hrp gene cluster of pseudomonas solanacearum gmi1000 strain encodes functions that are essential for pathogenicity on tomato and for the elicitation of the hypersensitive response on tobacco. in this study, we present the nucleotide sequence of one of the hrp genes (hrpb) located at the left-hand end of the cluster and we show that hrpb encodes a positive regulator controlling the expression of hrp genes. hrpb has a coding capacity for a 477-amino-acid polypeptide, which shows significant si ...19921479894
yersinia enterocolitica endocarditis on a prosthetic valve.yersinia entercolitica endocarditis has rarely been described before. this is the first report of prosthetic valve yersinia enterocolitis endocarditis, complicated by infected brain embolization. the patient, however, completely recovered after 6 weeks of combined therapy with ceftriaxone and gentamicin.19921480543
[associations of the soil amoeba hartmannella rhysodes with the bacterial causative agents of plague and pseudotuberculosis in an experiment].some aspects of relationships between soil ameba and the causative agents of plague and pseudotuberculosis, capable of forming natural associations, are considered. ameba can phagocytose bacteria causing plague and pseudotuberculosis. cases of the preservation of individual bacterial cells at the stationary phase and in precysts of amebae have been registered.19921481595
a method to compare exposed populations in seroepidemiology.in seroepidemiology when a single sample is taken from exposed and non-exposed subjects, the results of serological tests are usually reduced to a dichotomy: normal (low or no antibodies) or elevated (high or antibodies found). the choice of the cutoff point between these values has been based on various vaguely defined methods and the results depend, at least partly, on the method chosen. an alternative method is introduced here which is not based on the absolute value of a serological test but ...19921483826
the hha protein from escherichia coli is highly homologous to the ymoa protein from yersinia enterocolitica. 19921484495
yersinia-related arthritis in the united kingdom. a report of 12 cases and review of the literature.reactive arthritis following infection with yersinia is endemic in scandinavian countries; the prevalence is low in the uk, however. we have reviewed the literature pertaining to yersinia-related reactive arthritis in the uk and describe 12 patients who presented over a 3-year period with an asymmetrical seronegative polyarthropathy and serological evidence of recent yersinia infection. five patients recalled having a diarrhoeal illness prior to the onset of the arthropathy. none had a prior his ...19921484936
long term prognosis in yersinia arthritis: clinical and serological findings.twenty two patients were followed up for a mean of 10.7 years after acute yersinia arthritis. their clinical course, agglutination antibodies, antibodies against plasmid coded yersinia proteins, and laboratory parameters of inflammation were analysed. the clinical findings were unremarkable. serum agglutinating antibodies against yersinia enterocolitica were not found in any patient. ten patients had no antibodies against plasmid coded bacterial proteins. five patients showed igg antibodies, one ...19921485817
isolation of yersinia enterocolitica from foods.many selective enrichment and plating media for the isolation of yersinia enterocolitica from foods are described. however, at present no single isolation procedure is available for the recovery of all pathogenic strains of yersinia enterocolitica. cold enrichment in phosphate-buffered saline plus 1% sorbitol and 0.15% bile salts (pbssb) and two-step enrichment with tryptone soy broth (tsb) and bile oxalate sorbose (bos) broth are very efficient methods for the recovery of a wide spectrum of ser ...19921486026
in vitro characteristics of yersinia pseudotuberculosis of nonhuman primate origin.six strains of serotypes 1 or 2 of y. pseudotuberculosis were isolated from dead squirrel monkeys, a cotton-top tamarin and a marmoset hybrid. all strains harboured a 71.6 kb plasmid, all were totally oxacillin-resistant and partially resistant to cephalosporins. biochemically, serotypes 1 and 2 differed from each other in their beta-galactosidase production in a nonfermenter system, whereas the lack of rhamnose, maltose, salicin and trehalose fermentation seemed to be attributable to technical ...19921486229
yersinia enterocolitica serovar 1,2a,3 biovar 3 in chinchillas.yersinia enterocolitica (serovar 1,2a, 3; biovar 3, autoagglutination test positive) was isolated in a chinchilla, in which autopsy showed the typical lesions associated with pseudotuberculosis. we assume that this "chinchilla type" of yersinia enterocolitica still persists among stock, more than thirty years after its discovery. a brief overview of the distribution of the pathogen in europe and north america is given.19921486240
a rapid, simple method for detecting pcr failure. 19921490181
degenerate pcr primers for the amplification of fragments from genes encoding response regulators from a range of pathogenic bacteria.many bacterial responses to environmental stimuli are mediated by response regulators which coordinately regulate genes involved in particular adaptive responses. degenerate oligonucleotide primers were used to amplify by the polymerase chain reaction (pcr), fragments from genes encoding eleven novel response regulators. sequence and phylogenetic analysis revealed that phob, phop and creb gene fragments had been amplified from yersinia enterocolitica and yersinia pseudotuberculosis, and that a c ...19921490612
antibacterial spectrum of lactoferricin b, a potent bactericidal peptide derived from the n-terminal region of bovine lactoferrin.a physiologically diverse range of gram-positive and gram-negative bacteria was found to be susceptible to inhibition and inactivation by lactoferricin b, a peptide produced by gastric pepsin digestion of bovine lactoferrin. the list of susceptible organisms includes escherichia coli, salmonella enteritidis, klebsiella pneumoniae, proteus vulgaris, yersinia enterocolitica, pseudomonas aeruginosa, campylobacter jejuni, staphylococcus aureus, streptococcus mutans, corynebacterium diphtheriae, list ...19921490908
characterization of an amorphous and soluble hemagglutinin from yersinia pseudotuberculosis.yersinia pseudotuberculosis which were screened out depending on auto-agglutination and ca2+ dependency, were examined for their production of hemagglutinin (ha), and its purification and characterization were performed. the ha with a broad reactivity with various mammalian erythrocytes was recovered from the culture supernatant of these strains grown at 37 c but not 25 c. has from two strains, r148r and t1040, were purified by salt precipitation, gel filtration and anion-exchange chromatography ...19921491617
cytometric and cytochemical study of peritoneal and alveolar macrophages from yersinia pseudotuberculosis infected ground squirrels (citellus citellus).the enzymic activity (succinate dehydrogenase, acid and alkaline phosphatase) of alveolar and peritoneal macrophages as well as leucocytic reaction of ground squirrels infected with yersinia pseudotuberculosis (i and iii serovar) have been investigated in dynamics from the 1st up to the 30th day. the animals infected with iii serovar survived only to the 7th day, while those infected with i serovar survived up to the 30th day after inoculation. a massive influx of leucocytes having peak values ( ...19921492517
kikuchi's lymphadenitis: report of a yersinia enterocolitica-associated case and an overview of aetiology and clinical outcome.kikuchi's lymphadenitis (kl; histiocytic necrotizing lymphadenitis without granulocytic infiltration) is a generally benign, febrile disorder of unknown aetiology with distinct histological features. to date, a minority of cases reported have been associated with infectious agents. a typical pathological case of kl is described where involvement of yersinia enterocolitica was shown by an indirect immunofluorescent assay applied to lymphatic tissue. the case is discussed with emphasis on recent i ...19921494401
[the possibility of yersinia pseudotuberculosis transmission via the soil-plant-animal chain (a preliminary report)]. 19921496869
[pasteurized milk as a factor in the transmission of the causative agents of yersiniosis].from 43 (35.8%) out of 120 samples of pasteurized milk used by volunteers 182 cultures of five yersinia species--y. enterocolitica, biovar 1 (70.3%), y. intermedia (18.1%), y. frederiksenii (7.7%), y. kristensenii (3.3%) and y. aldovae (0.5%)--were isolated in the course of bacteriological studies carried out in 1987-1990. the isolated yersinia cultures belonged to nonpathogenic pyraminidaze-positive serovars. yersinia pathogenic bio/serovars (4/o3, 2/o9, 1b/o8) were not detected. no deviations ...19921496874
[experience with the use of an immunoglobulin erythrocyte preparation for the early laboratory diagnosis of pseudotuberculosis].an immunoglobulin erythrocytic preparation for the rapid detection of yersinia pseudotuberculosis was developed. the preparation was shown to be specific and sufficiently sensitive (8 x 10(5) microbial cells/ml). the preparation was used for the examination of 102 patients; in 62 of these patients the diagnosis was confirmed by means of common laboratory and clinical methods. y. pseudotuberculosis antigen was detected in the blood of 39 patients (63%), and in 12 patients (29.4%) the results of t ...19921496881
experimental yersinia enterocolitica placentitis in sheep.a strain of yersinia enterocolitica of o serogroup 6,30 isolated from the liver of an aborted ovine fetus was inoculated intravenously into a group of pregnant ewes at about 90 days gestation and produced placentitis with abortion or delivery of infected lambs about 50 days later. y. enterocolitica of the same serogroup was recovered from the necrotic placental cotyledons and most other fetal tissues and could be isolated from vaginal discharges of the ewes for a least 2 weeks after abortion. hi ...19921498646
expression of the eukaryotic trypanosoma cruzi cra gene in yersinia enterocolitica and induction of an immune response against cra in mice.the 70-kb plasmid pyv of yersinia enterocolitica directs the secretion of a set of proteins, called yops, that are produced during infection of humans and animals. trypanosoma cruzi, the agent of american trypanosomiasis, synthesizes a cytoplasmic protein termed cra that is considered to be t. cruzi specific. to produce cra in y. enterocolitica, we constructed a hybrid yope-cra gene that we integrated into plasmid pyv by homologous recombination. recombinant y. enterocolitica produced a chimeric ...19921500192
the integrin-binding domain of invasin is sufficient to allow bacterial entry into mammalian cells.yersinia pseudotuberculosis is able to enter normally nonphagocytic host cells by multiple pathways, the most efficient of which is mediated by invasin, a 986-amino-acid bacterial outer membrane protein. it has previously been shown that the c-terminal 192 amino acids of invasin are sufficient to bind mammalian cells. to determine if additional regions of the invasin protein are necessary to promote entry, we developed a novel assay that tests the ability of various invasin derivatives to confer ...19921500198
polymerase chain reaction-gene probe detection system specific for pathogenic strains of yersinia enterocolitica.the polymerase chain reaction technique was used to develop a rapid diagnostic assay for detection of pathogenic yersinia enterocolitica strains. the assay targeted a stretch of 163 bp of the yst gene and could be applied to both pure cultures and crude dna extracted from feces. the defined primer pair amplified the targeted sequence from only pathogenic strains and fecal samples seeded with the serotype o:3 strain of y. enterocolitica, whereas neither nonpathogenic strains nor normal stools yie ...19921500496
in vitro and in vivo antibacterial activities of levofloxacin (l-ofloxacin), an optically active ofloxacin.the antibacterial activity of levofloxacin was compared with those of ofloxacin, ciprofloxacin, and other antibiotics. in general, levofloxacin was equally active or up to fourfold more active than ofloxacin against all 801 organisms tested. levofloxacin was twofold [corrected] more active than ciprofloxacin against streptococcus pneumoniae and 2- to 4-fold more active than ciprofloxacin against staphylococcus aureus, xanthomonas maltophilia, and bacteroides fragilis. levofloxacin was two- to ei ...19921503449
[yersiniosis].the authors' objective in the above review was to stress the epidemiological importance of yersinia enterocolitica infections. they therefore detail the clinical and epidemiologic features of acute or primary as well as of secondary forms and complications of yersinia enterocolitica infections. further, they dwell on the management of these infections and illustrate the properties, toxic effects and other side effects of the antibiotics commonly used in therapy and for the prevention of complica ...19921505177
[plasmid composition of yersinia pestis strains from different natural foci].the study of the plasmid composition of 246 y. pestis strains from different natural foci in the ussr and other countries revealed that 173 strains (70%) carried three known plasmids with a molecular weight of about 6, 45-50 and 60 megadaltons (md) respectively. in 20 strains (8%) obtained from different sources additional cryptic plasmids were detected. in some cases the absence of one or two typical plasmids was observed. replicon ppst was shown to have quite constant molecular weight (6 md), ...19921509839
nucleotide sequence of the ampc-ampr region from the chromosome of yersinia enterocolitica.the nucleotide sequence of a 3.1-kb region from the chromosome of the yersinia enterocolitica o:5b strain ip97 containing the gene for an inducible chromosomal cephalosporinase has been determined. the cephalosporinase gene was homologous to other enterobacterial chromosomal cephalosporinase genes, and it was accompanied by a gene homologous to the regulatory ampr gene. the arrangement of genes in the y. enterocolitica ampcr unit was identical to that in the enterobacter cloacae and citrobacter ...19921510392
serologic survey for selected arboviruses and other potential pathogens in wildlife from mexico.during 1988 and 1989, a serologic survey of wildlife was conducted in northeastern mexico to determine the presence, prevalence, and distribution of arboviruses and other selected disease agents. eighty mammal specimens were tested. antibodies to vesicular stomatitis-indiana, venezuelan equine encephalitis-mena ii, rio grande virus, and vesicular stomatitis-new jersey were detected predominantly in small mammals. deer and mouflon (ovis musimon) had antibodies to bluetongue and epizootic hemorrha ...19921512876
differentiation between pathogenic and non-pathogenic yersinia enterocolitica strains by colony hybridization with a pcr-mediated digoxigenin-dutp-labelled probe.the polymerase chain reaction (pcr) method was used to generate a vector-free digoxigenin-dutp labelled probe that targets the yersinia enterocolitica gene encoding the heat stable enterotoxin (yst). the probe was used in dna-dna colony hybridization to screen 113 strains of y. enterocolitica and related species for the presence of the enterotoxin gene. in y. enterocolitica, the probe clearly discriminated between pathogenic and non-pathogenic strains even those belonging to the same serotype. o ...19921513345
direct isolation of yersinia pseudotuberculosis from fresh water in japan.yersinia pseudotuberculosis was recovered from koh-treated precipitates of 20.6% of 500 freshwater samples. koh treatment of precipitates is a simple and expedient means of recovering y. pseudotuberculosis from such samples.19921514818
autologous transfusion-transmitted yersinia enterocolitica. 19921518105
alteration in t cell/macrophage ratio may reveal lymphocyte proliferation specific for the triggering antigen in reactive arthritis.it has previously been shown that synovial fluid (sf) mononuclear cells (mnc) from patients with reactive arthritis (rea) and some patients with undifferentiated oligoarthritis (uoa) respond specifically to the triggering bacterium (specific responders). however, in some patients there is a response to two or more bacteria (non-specific responders) and in a third group no response is found (non-responders). we assessed whether the proportion of synovial mnc which were macrophage-monocyte (mamo) ...19921519037
yersinia enterocolitica and white cell filtration. 19921519322
the role of white cells in the transmission of yersinia enterocolitica in blood components.the mechanism for the transmission of yersinia enterocolitica in blood components has been studied experimentally. one hypothesis is that, during a yersinia infection in the blood donor, bacteria are phagocytosed by white cells (wbcs), but are not killed. after collection of blood from such a donor and component production, the bacteria are present in wbcs for some time, during which the unit appears sterile. later, when the wbcs disintegrate, the bacteria are released and multiply in the unit. ...19921519328
prevention of growth of yersinia enterocolitica in blood by polyester fiber filtration.the ability of polyester white cell-reduction blood filters to prevent the growth of yersinia enterocolitica in units of donated blood was studied. sixteen units of freshly drawn blood were inoculated with 10, 50, 100, or 150 colony-forming units (cfu) per ml of a clinical isolate of y. enterocolitica (serotype o:3). the units were subsequently fractionated into red cell concentrate and resuspended in as-1 or as-3 solution. one-half of the red cell concentrates in each solution were filtered wit ...19921519329
removal of yersinia enterocolitica from as-1 red cells.the growth of yersinia enterocolitica in as-1 red cells was investigated so as to study the organism's proliferation kinetics and to evaluate the effect of prestorage white cell (wbc) reduction on bacterial multiplication. twenty-four 2-unit pools of abo-compatible whole blood were prepared and inoculated with y. enterocolitica to final concentrations ranging from 0.3 to 132 organisms per ml. after inoculation, pools were split equally, as-1 red cells were prepared, and 1 unit of each pair (test ...19921519330
growth of yersinia species in artificially contaminated blood bags.transfusion blood bags were inoculated with less than 0.1 colony-forming units (cfu) of yersinia enterocolitica (serotypes o:3, o:5, and o:9) and yersinia pseudotuberculosis i per ml and stored at 4 degrees c for 41 days. during storage, samples were collected periodically and colony counts of serial dilutions were performed by plating on tryptic soy agar. after a lag-phase, the growth of yersinia species from less than 0.1 cfu per ml to 10(9) cfu per ml was observed in 3 of 10 bags. the data sh ...19921519331
essential arginines in mercuric reductase isolated from yersinia enterocolitica 138a14.the mercuric reductase from yersinia enterocolitica 138a14 was inactivated by the arginine modifying reagents 2,3-butanedione and phenylglyoxal. the inactivation by 2,3-butanedione exhibited second order kinetics with rate constant of 32 min-1 m-1. in the case of phenylglyoxal, biphasic kinetics were observed. the oxidized coenzyme (nadp+) prevented inactivation of the enzyme by the alpha-dicarbonyl reagents, whereas the reduced coenzyme (nadph) enhanced the inactivation rate. the loss of enzyme ...19921520735
laboratory investigation of virulence among strains of yersinia enterocolitica and related species isolated from pigs and pork products.eighty strains of yersinia enterocolitica and related species isolated from slaughtered pigs and pork products were tested for possession of virulence-associated phenotypes by employing 12 in vivo and in vitro assays. the isolates could be broadly divided into two groups: (i) strains belonging to pathogenic bioserotypes of y. enterocolitica that displayed virulence-associated characteristics in most or all assays and (ii) strains belonging to y. enterocolitica biotype 1a and to related species t ...19921521193
evidence that bismuth salts reduce invasion of epithelial cells by enteroinvasive bacteria.the effects of sublethal concentrations of bismuth salts on bacterial invasion of mammalian cells were investigated. pepto-bismol, bismuth subsalicylate, and bismuth oxychloride, produced by interacting bismuth subsalicylate and simulated gastric juice, in suspension at concentrations as low as 1.4 mm significantly interfered with the invasion of rpmi-4788 cells by two different strains of yersinia enterocolitica. invasion of the mammalian epithelial cells by other enteric bacteria was also redu ...19921522823
deposition of bismuth by yersinia enterocolitica.yersinia enterocolitica 8081c cultures in exponential growth were incubated for 1 h in 0.1% microcrystalline bismuth subsalicylate (bss) suspensions. scanning electron microscopy (sem) revealed microcrystals directly bound to bss-treated bacteria. energy dispersive spectroscopy (eds) x-ray microanalysis of the attached microcrystals confirmed that the crystals were the microcrystalline bss. x-ray spectra positive for bismuth were also obtained by sem-eds x-ray microanalysis of whole bacteria, su ...19921522824
experimental yersinia enterocolitica infection in rats: analysis of the immune response to plasmid-encoded antigens of arthritis-susceptible lewis rats and arthritis-resistant fischer rats.recently, experimentally yersinia-induced arthritis in lewis and shr rats has been described as a potential animal model for analyzing the pathomechanism of reactive arthritis (toivanen et al. 1986; hill and yu 1987). we could confirm that lewis but not fischer rats develop aseptic arthritis about 2 weeks after an intravenous inoculation of yersinia enterocolitica, serotype 0:8 (hill and yu 1987). moreover, we compared the antibody response to virulence-associated yersinia antigens (yops) of exp ...19921522826
yersinia infections in patients with homozygous beta-thalassemia associated with iron overload and its treatment.patients with homozygous beta-thalassemia are at increased risk of serious infections. yersinia enterocolitica is an organism with a predilection for these and other iron-overloaded patients. three young adult patients with beta-thalassemia who were chronically transfused and developed yersiniosis are reported. iron overload and desferrioxamine use are predisposing factors, as supported by clinical, animal, and in vitro data. iron excess both immunologically compromises the host and greatly enha ...19921525003
structure of the repeating unit of the o-specific polysaccharide of the lipopolysaccharide of yersinia kristensenii strain 490 (o:12,25).the o-specific polysaccharide isolated by mild acid degradation of the lipopolysaccharide of y. kristensenii strain 490 (o:12,25) contained d-glucose, 2-acetamido-2-deoxy-d-glucose, 2-acetamido-2-deoxy-d-galactose, 2-acetamido-2,6-dideoxy-l-galactose, glycerol, and phosphate in the ratios 2:2:1:1:1:1. on the basis of 31p- and 13c-n.m.r. data, methylation analysis, dephosphorylation, solvolysis with anhydrous hydrogen fluoride, and smith degradation, it was concluded that the repeating unit of th ...19921525785
reactive arthritis: the role of bacterial antigens in inflammatory arthritis.for more than 100 years it has been suspected that bacteria or products derived from them are deposited in joints and cause arthritis without suppuration. over this time a vast amount of evidence, much of which is still unchallenged, has accumulated to demonstrate that whole bacteria and subcellular bacterial elements do pass, under certain circumstances, from sites of mucosal colonization or infection into the circulation and thence into joints. similarly, experimental studies have demonstrated ...19921525841
clinical features of sporadic yersinia enterocolitica infections in norway.during october 1988 through january 1990, a study of sporadic yersinia enterocolitica infections was done in the oslo region to assess the clinical impact and risk factors for this disease. sixty-seven case-patients (mean age, 23.4 years) and 132 population-based age- and sex-matched controls were enrolled. among patients who were well when interviewed, illness lasted a mean of 20 days, but 10% of the others remained symptomatic a year later. bloody diarrhea occurred only in persons less than 18 ...19921527416
adhesive properties conferred by the plasminogen activator of yersinia pestis.a genomic library of yersinia pestis ev76c created in a cosmid vector was screened for clones capable of binding type iv collagen. an unexpectedly high number of such clones was observed. one recombinant plasmid was selected for further study, and the locus controlling collagen binding was mapped by subcloning, transposon mutagenesis and exonuclease digestion. the outer-membrane protein profiles of transposon insertion mutants were correlated with phenotype to implicate a 36 kda polypeptide in t ...19921527508
identification of invasive yersinia species using oligonucleotide probes.oligonucleotide probes directed to the inv and ail invasion genes of yersinia species were used to analyse yersiniae and non-yersiniae isolates by colony hybridization. the inv-3 probe, targeted to the inv gene of yersinia pseudotuberculosis, hybridized with all 48 hela cell-invasive y. pseudotuberculosis isolates examined; the pf-13 probe, specific for the ail gene of yersinia enterocolitica, identified all invasive strains (36 of 52) of y. enterocolitica tested. neither probe hybridized with n ...19921528199
yersinia enterocolitica causing pneumonia and empyema in a child and a review of the literature. 19921528652
yersinia pseudotuberculosis and ovine abortion. 19921529512
[thymidine phosphorylase activity and its relationship to trimethoprim in initial strains and thymidine-, thymine-dependent and trimethoprim-resistant mutants of plague microbe].the comparative study revealed thymidine phosphorylase activity in the initial strains of a plague microbe of the field variety and in thymidine-, thymine-dependent and trimethoprim-resistant mutants of the plague microbe of other varieties. the data fully conformed to the results of the microbiological investigation of the strains' ability to grow on the nutrient media with trimethoprim in the presence of thymine and thymidine. on the basis of these results it appeared possible to divide the in ...19921530352
does reduced erythrocyte c3b receptor (cr1) activity contribute to the pathogenesis of yersinia triggered reactive arthritis?erythrocyte c3b receptor (cr1) activity was measured in 27 patients with yersinia triggered reactive arthritis and in 151 control subjects, including 36 patients with uncomplicated yersiniosis and 115 healthy subjects. cr1 was measured by the immune adherence haemagglutination method. patients with yersinia triggered reactive arthritis had reduced levels of cr1 compared with the controls. this difference was mainly due to the finding that five out of six hla b27 negative patients with arthritis ...19921531747
yersinia enterocolitica activates a t helper type 1-like t cell subset in reactive arthritis.the profile of lymphokines secreted by 14 t cell clones and 24 t cell lines reactive with yersinia ag isolated from the synovial fluid cells of two hla-b27+ patients with yersinia-triggered reactive arthritis was characterized. in response to ag-specific or -nonspecific stimulation, all of the yersinia-reactive t cell clones and lines had a pattern of lymphokine secretion resembling that of murine (th1) cells. a total of 50% of t cell lines and clones randomly isolated from a reactive arthritis ...19921533651
[a 2-year study of the incidence of campylobacteriasis in the district of liptovský mikulás].the authors investigated the incidence of campylobacteriosis in the population of the district during 1989-1990. during the above period by cultivation 723 cases of the disease were confirmed, i.e. the incidence in 1989 was 319, the number of cases 428, in 1990 the corresponding figures were 220 and 295. the incidence of campylobacteriosis is significantly higher than that of other investigated bacterial diseases. the authors did not find a significant difference in the incidence of campylobacte ...19921534519
are intestinal bacteria involved in the etiology of rheumatoid arthritis? review article.observations in bowel-related joint diseases give support to this hypothesis. in crohn's disease and ulcerative colitis, the bowel wall inflammation is complicated in about 20% of the patients by joint inflammation. bowel infection by salmonella, shigella and yersinia can provoke joint inflammation and supports an etiological link between bowel bacteria and arthritis. the arthropathic properties of the most abundant group of intestinal bacteria, i.e. the obligate anaerobic bacteria, were studied ...19921536716
mechanistic studies of the biosynthesis of 3,6-dideoxyhexoses in yersinia pseudotuberculosis: purification and characterization of cdp-4-keto-6-deoxy-d-glucose-3-dehydrase.cdp-4-keto-6-deoxy-d-glucose-3-dehydrase (e1) is a pmp-dependent enzyme which plays an essential role in c-o bond cleavage leading to the formation of 3,6-dideoxyhexoses. although e1 catalysis has long been recognized as a unique biological deoxygenation reaction, the catalytic mechanism of this unusual enzyme has never been fully elucidated. the lack of methods that would allow this enzyme's activity to be monitored directly has been an impediment to e1 purification and has consequently hampere ...19921536853
mechanistic and stereochemical studies of a unique dehydration catalyzed by cdp-4-keto-6-deoxy-d-glucose-3-dehydrase: a pyridoxamine 5'-phosphate dependent enzyme isolated from yersinia pseudotuberculosis.cdp-4-keto-6-deoxy-d-glucose-3-dehydrase (e1) purified from yersinia pseudotuberculosis is a pyridoxamine 5'-phosphate (pmp) dependent enzyme which catalyzes the c-o bond cleavage at c-3 of a cdp-4-keto-6-deoxy-d-glucose substrate, a key step in the formation of 3,6-dideoxyhexoses. since enzyme e1 utilizes the pmp cofactor in a unique manner, it is essential to establish its role in e1 catalysis. when an incubation was conducted in [18o]h2o, incorporation of 18o into positions c-3 and c-4 of the ...19921536854
yersinia enterocolitica mesenteric adenitis in a thalassaemic adolescent. conservative management. 19921537358
yersinia enterocolitica enteritis affects rabbit intestinal longitudinal smooth muscle function.to determine whether yersinia enterocolitica (ye) enteritis has an effect on the biomechanical properties of intestinal smooth muscle, new zealand white rabbits (600-900 g) were divided into an infected group (n = 9) and sham-infected animals fed ad libitum (n = 9), or pair fed with the infected group (n = 9). animals were inoculated with 10(10) organisms of ye in 10 ml nahco3 (infected group) or 10 ml nahco3 (sham-infected control and pair-fed groups) at time 0. daily food intake, weight gain, ...19921539661
long term follow up of shr rats with experimental yersinia associated arthritis.one hundred and one shr rats were injected intravenously with live yersinia enterocolitica o:8. the rats were randomly divided into two groups consisting of 48 and 53 rats. the group of 48 rats was monitored for 245 days to establish the incidence and the clinical features of the arthritis. the remaining 53 rats were killed in groups of three to five rats at intervals from four to 245 days after inoculation to examine the clearance of bacteria and the development of histological changes in the s ...19921540047
t lymphocytes mediate protection against yersinia enterocolitica in mice: characterization of murine t-cell clones specific for y. enterocolitica.yersinia enterocolitica is enteropathogenic for humans and rodents, causing intestinal and extraintestinal diseases. the cellular immune response of the infected host has not yet been analyzed in detail. therefore, we used a parenteral mouse infection model to determine the role of t lymphocytes in immunity against y. enterocolitica. we report the generation and characterization of y. enterocolitica-specific t-cell clones isolated from spleens of intravenously infected c57bl/6 mice. the t-cell c ...19921541529
listeria monocytogenes, yersinia enterocolitica, and salmonella in dairy plant environments.in order to determine the presence of the three environmental pathogens in dairy plants, six milk and four ice cream plants in a three state area were sampled. a total of 353 environmental samples were taken over three replications. bacterial counts were performed on the environmental samples for chi-square analysis. salmonella spp. were not isolated from any of the environmental samples. yersinia enterocolitica was isolated from 6.8% of the environmental samples. listeria monocytogenes was isol ...19921541741
yersinia and blood donation. 19921542930
acute and chronic gastrointestinal manifestations associated with yersinia enterocolitica infection. a norwegian 10-year follow-up study on 458 hospitalized patients.the aim of the present study was to elucidate the gastrointestinal manifestations of yersiniosis. during the period 1974 to 1983, yersinia enterocolitica infection was diagnosed in 458 patients, by isolation from fecal samples or by antibody response. at first admission, 184 patients had abdominal pain; 200, diarrhea; 45, vomiting; and 36, weight loss. ulcerative colitis was diagnosed in 7 patients, crohn's disease in 2, and unspecific colitis in 11. mesenteric lymphadenitis or ileitis were foun ...19921543397
bubonic plague.a 19-year-old man, recently returned from a 10-day military exercise in central california, had acute onset of shaking chills, headache, and bilateral inguinal adenopathy after having been bitten by insects on his lower extremities. he had exquisitely tender inguinal and femoral nodes bilaterally. needle aspirate from an inguinal node grew yersinia pestis. the patient was treated with streptomycin and chloramphenicol and did well.19921546359
neutrophil, glass-adherent, nitroblue tetrazolium assay gives early indication of immunization effectiveness in rainbow trout.neutrophil activity in rainbow trout (oncorhynchus mykiss) is increased upon antigenic stimulation with the yersinia ruckeri o-antigen bacterin. the characteristics of neutrophil attachment to glass and nitroblue tetrazolium (nbt) staining were used to determine the effectiveness of immunization programs with fingerling rainbow trout. fish immunized by intraperitoneal injection with doses of 100, 10, or 1 microgram of the bacterin showed the highest responses in that order in numbers of glass ad ...19921546446
the serological relationship between escherichia coli o157 and yersinia enterocolitica o9 using sera from patients with brucellosis.sera from ten patients with positive brucella serology were used to investigate antibody cross-reactions between the o-antigens of escherichia coli o157 and yersinia enterocolitica o9. sds-page profiles of lipopolysaccharide (lps), purified from strains of e. coli o157 and y. enterocolitica o9, were reacted with sera by immunoblotting. all ten sera contained antibodies which bound to the lps of e. coli o157, and five of these sera also contained antibodies which bound to the lps of y. enterocoli ...19921547843
use of recombinant interleukin-2 to enhance adoptive transfer of resistance to listeria monocytogenes infection.in vitro incubation of listeria-immune spleen cells (lisc) with recombinant interleukin-2 (ril-2) for at least 3 days increased their ability to transfer antilisteria resistance to recipient mice. this effect was blocked by the in vitro addition of transforming growth factor beta 1. the level of protection afforded by the transfer of ril-2-incubated lisc was further elevated by the in vivo administration of ril-2 at a dose that by itself did not significantly increase antilisteria resistance. th ...19921548066
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