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volatiles emitted from eight wound-isolated bacteria differentially attract gravid screwworms (diptera: calliphoridae) to oviposit.bovine blood inoculated with bacteria isolated from screwworm [cochliomyia hominivorax (coquerel) (diptera: calliphoridae)]-infested animal wounds was tested as an attractant for oviposition for gravid screwworms. eight species of gram-negative coliform (enterobacteriaceae) bacteria mixed with bovine blood singly or all species combined and incubated for various times produced volatiles that attracted gravid flies in a cage bioassay in varying numbers. in 15-min duration tests, volatiles from fi ...201020496582
female and male genetic contributions to post-mating immune defence in female drosophila melanogaster.post-mating reduction in immune defence is common in female insects, and a trade-off between mating and immunity could affect the evolution of immunity. in this work, we tested the capacity of virgin and mated female drosophila melanogaster to defend against infection by four bacterial pathogens. we found that female d. melanogaster suffer post-mating immunosuppression in a pathogen-dependent manner. the effect of mating was seen after infection with two bacterial pathogens (providencia rettgeri ...201020573620
phylogenetic analysis of the genera proteus, morganella and providencia by comparison of rpob sequences of type and clinical strains suggests the classification of proteus myxofaciens in a new genus cosenzaea gen. nov., as cosenzaea myxofaciens comb. nov.phylogenetic analysis of partial rpob gene sequences of type and clinical strains belonging to different 16s rrna gene-fingerprinting ribogroups within 11 species of enterobacteria of the genera proteus, morganella, and providencia allowed to define rpob clades supported by high bootstrap values and confirmed by >/=2.5% nucleotide divergence. no rpob clade included strains belonging to different species and the majority of the species were confirmed as discrete and homogeneous, since they consti ...201020709916
alimentary tract bacteria isolated and identified with api-20e and molecular cloning techniques from australian tropical fruit flies, bactrocera cacuminata and b. tryoni.bacteria were isolated from the crop and midgut of field collected bactrocera cacuminata (hering) and bactrocera tryoni (froggatt) (diptera: tephritidae). two methods were used, firstly isolation onto two types of bacteriological culture media (pyea and tsa) and identification using the api-20e diagnostic kit, and secondly, analysis of samples using the 16s rrna gene molecular diagnostic method. using the api-20e method, 10 genera and 17 species of bacteria in the family enterobacteriaceae were ...201020883132
xanthogranulomatous pyelonephritis with nephrocutaneous fistula due to providencia rettgeri infection.we describe what is to our knowledge the first case of xanthogranulomatous pyelonephritis combined with nephrocutaneous fistula caused by providencia rettgeri. surgical extirpation including nephrectomy and fistulectomy was successfully performed. the strain was identified by 16s rrna gene sequencing in both renal tissue and pus culture from the fistula.201121459904
vim and imp metallo-β-lactamases and other extended-spectrum β-lactamases in escherichia coli and klebsiella pneumoniae from environmental samples in a tunisian hospital.an extremely drug-resistant enterobacteriaceae species emerged in kasserine hospital, tunisia between 2009 and 2010 causing a local outbreak. we aimed to characterize extended-spectrum β-lactamase (esbl) and metallo-β-lactamase (mbl)-producing enterobacteriaceae from the hospital environment. swabs were collected from ten different wards from kasserine hospital, tunisia. a total of 46 isolates were cultured onto macconkey agar supplemented with ceftazidime to select for esbl-producing enterobact ...201121917010
Effective cohorting and "superisolation" in a single intensive care unit in response to an outbreak of diverse multi-drug-resistant organisms.Cohorting patients in dedicated hospital wards or wings during infection outbreaks reduces transmission of organisms, yet frequently, this may not be feasible because of inadequate capacity, especially in the intensive care unit (ICU). We hypothesized that cohorting isolation patients in one geographic location in a single ICU and using enhanced isolation procedures ("superisolation") can prevent the further spread of highly multi-drug-resistant organisms (MDRO).201121936667
Description of a 2,683-Base-Pair Plasmid Containing qnrD in Two Providencia rettgeri Isolates.qnr genes are plasmid-mediated quinolone resistance genes mainly harbored on large conjugative multiresistant plasmids. The qnrD gene was recently observed in Salmonella enterica on a small nonconjugative plasmid (p2007057). We describe two strains of Providencia rettgeri harboring qnrD on nonconjugative plasmids. The plasmids were 99% identical, with 2,683 bp and four open reading frames, including qnrD, but exhibited only 53% identity with the plasmid found in S. enterica.201221986831
synthesis and evaluation of antimicrobial activity of 4h-pyrimido[2,1-b]benzothiazole, pyrazole and benzylidene derivatives of curcumin.a novel, one-pot, simple, efficient procedure for 4h-pyrimido[2,1-b]benzothiazole (4a-h), pyrazole (6a-d) and benzylidene (7a-d) derivatives of curcumin under solvent and solvent free conditions in microwave with good yield is have been synthesized. the synthesized compounds were evaluated for their antibacterial activity against gram-positive and gram-negative bacteria viz. staphylococcus aureus, pseudomonas aeruginosa, salmonella typhi, escherichia coli, bacillus cereus and providencia rettger ...201222683240
yersinia enterocolitica in sheep--a high frequency of biotype 1a.pigs are regarded as the main reservoir for human pathogenic yersinia enterocolitica, which is dominated by bioserotype 4/o:3. other animals, including sheep, have occasionally been reported as carriers of pathogenic strains of y. enterocolitica. to our knowledge, this is the first study performed in the nordic countries in which the presence of y. enterocolitica in sheep is investigated.201222748116
fluoroquinolone-resistant uncomplicated urinary tract infections, chinese herbal medicine may provide help.we assessed the effects of chinese herbs on the uncomplicated urinary tract infections (utis) in women caused by fluoroquinolone-resistant strains. a total of 56 pre-menopausal women with uncomplicated utis caused by fluoroquinolone-resistant strains were included. urine cultures were carried out. all organisms were proved to be fluoroquinolone-resistant at baseline. the patients were orally administrated chinese herbal concoction for ten days. chinese herbal concoction eradicated the primary pa ...201122754063
no effect of wolbachia on resistance to intracellular infection by pathogenic bacteria in drosophila melanogaster.multiple studies have shown that infection with the endosymbiotic bacterium wolbachia pipientis confers drosophila melanogaster and other insects with resistance to infection by rna viruses. studies investigating whether wolbachia infection induces the immune system or confers protection against secondary bacterial infection have not shown any effect. these studies, however, have emphasized resistance against extracellular pathogens. since wolbachia lives inside the host cell, we hypothesized th ...201222808174
biosorption of aluminum, cobalt, and copper ions by providencia rettgeri isolated from wastewater.twenty-three bacterial isolates from polluted water and soil were screened for heavy metals resistance (i.e., al(3+), co(2+), and cu(2+)). the most potent isolate was identified by morphological characteristics, biochemical tests and confirmed by api20e kits as providencia rettgeri mam-4. removal of al(3+) from aqueous solution by p. rettgeri is more efficient (∼fourfold) than that by b. cereus atcc 11778 (a comparison strain) at concentration of 200 mg l(-1) al(3+). p. rettgeri was able to remo ...201322915136
spread of ctx-m-15 positive providencia spp. causing urinary tract infections at the university hospital split in croatia.during 2010-2011, six providencia spp. (five providencia stuartii and one providencia rettgeri) urine isolates with unusual resistance phenotype were collected from various hospital units at the university hospital split in croatia. the aim of the study was to analyze the mechanisms of resistance to expanded-spectrum cephalosporins.201222926370
high load of multi-drug resistant nosocomial neonatal pathogens carried by cockroaches in a neonatal intensive care unit at tikur anbessa specialized hospital, addis ababa, ethiopia. 201222958880
prevalence of providencia strains among children with diarrhea in japan.in the present study, we examined the prevalence of providencia spp. strains among children with diarrhea in japan. we developed a providencia genus-specific polymerase chain reaction (pcr) method, and the specificity and sensitivity was evaluated to be 100% with various bacterial strains including 7 genera and 13 species. five of 345 samples (1.4%) were positive by pcr using a providencia genus-specific primer targeting the 16s rrna gene. the single species providencia rettgeri was isolated fro ...201223183210
granulomatous pneumonia and hepatitis associated with providencia rettgeri infection in a crocodile monitor lizard (varanus salvadorii).the present report describes a case of granulomatous pneumonia and hepatitis in a male crocodile monitor lizard (varanus salvadorii). during the necropsy of the monitor lizard, multifocal to coalescing pale yellow lesions were observed in both lung lobes, as well as similar, though milder, changes in the liver, and an ulcerative lesion on the food pad of the right hindlimb. histopathologically, the presence of multiple necrotising, chronic granulomas containing bacterial clumps were observed in ...201323439291
isolation of genetically unrelated bla(ndm-1)-positive providencia rettgeri strains in israel. 201323486709
distribution of extended-spectrum β-lactamases, ampc β-lactamases, and carbapenemases among enterobacteriaceae isolates causing intra-abdominal infections in the asia-pacific region: results of the study for monitoring antimicrobial resistance trends (smart).the increasing trend of β-lactam resistance among enterobacteriaceae is a worldwide threat. enterobacteriaceae isolates causing intra-abdominal infections (iai) from the study for monitoring antimicrobial resistance trends (smart) collected in 2008 and 2009 from the asia-pacific region were investigated. detection of extended-spectrum β-lactamases (esbls), ampc β-lactamases, and carbapenemases was performed by multiplex pcr. a total of 699 enterobacteriaceae isolates with positive genotypic resu ...201323587958
isolation of carbapenem-resistant ndm-1-positive providencia rettgeri in mexico. 201323620464
[investigation of the presence of new delhi metallo-beta-lactamase-1 (ndm-1) by pcr in carbapenem-resistant gram-negative isolates].bacteria producing new delhi metallo-beta-lactamase-1 (ndm-1) exhibit high level resistance to beta-lactams including carbapenems. this broad-spectrum resistance limits treatment options for infections caused by ndm-1 producers. ndm-1 was first isolated from an indian patient in sweden; since then, ndm-1 producing isolates have been identified in many countries including turkey. in this study, we investigated the presence of ndm-1 by pcr method in various gram-negative isolates recovered from cl ...201323621739
providencia rettgeri: an unusual cause of central nervous system infections.providencia, unlike other enterobacteriaceae, is a lesser known causative agent of hospital-acquired and community-acquired neuroinfection. two cases of meningitis and one patient with subdural empyema, where providencia rettgeri was the causative agent, are reported. to the authors' knowledge, this is the first report of hospital-acquired and community-acquired meningitis or empyema caused by p rettgeri.201323811573
isolation of ndm-producing providencia rettgeri in brazil. 201323869051
a treatment plant receiving waste water from multiple bulk drug manufacturers is a reservoir for highly multi-drug resistant integron-bearing bacteria.the arenas and detailed mechanisms for transfer of antibiotic resistance genes between environmental bacteria and pathogens are largely unclear. selection pressures from antibiotics in situations where environmental bacteria and human pathogens meet are expected to increase the risks for such gene transfer events. we hypothesize that waste-water treatment plants (wwtps) serving antibiotic manufacturing industries may provide such spawning grounds, given the high bacterial densities present there ...201324204801
complete sequences of a novel blandm-1-harbouring plasmid from providencia rettgeri and an fii-type plasmid from klebsiella pneumoniae identified in canada.emergence of plasmids harbouring bla(ndm-1) is a major public health concern due to their association with multidrug resistance and their potential mobility.201424275114
effects of methanol extract of wedelia chinensis osbeck (asteraceae) leaves against pathogenic bacteria with emphasise on bacillus cereus.the antibacterial activity of the methanol extract of wedelia chinensis leave was studied and tested against three pathogenic gram positive bacteria (bacillus cereus, b. subtilis and stapylococcus aureus) and three pathogenic gram negative bacteria (escherichia coli, proteus rettgeri and pseudomonas aeruginosa) by the disk diffusion assay and broth dilution methods. the extract exhibited favourable antibacterial activity against the bacterial cells but was more potent against gram positive bacte ...201324403653
ndm-1-producing strains, family enterobacteriaceae, in hospital, beijing, china. 201424456600
detection of tetrodotoxin-producing providencia rettgeri t892 in lagocephalus pufferfish.tetrodotoxin (ttx) is a potent toxin but it could be used in pharmaceutical field. identification of ttx producing bacteria in pufferfish is necessary for ttx yield and the pufferfish conservation. in this study, lagocephalus was collected from cam ranh sea, a central part of vietnam during spring season. the liver and intestine were incubated in 0.9 % nacl for ttx detection in pufferfish. to be benefited from the isolation of new ttx producing bacteria, the liver and intestine were incubated in ...201424458876
ndm-1 metallo-β-lactamase and arma 16s rrna methylase producing providencia rettgeri clinical isolates in nepal.drug-resistant providencia rettgeri producing metallo-β-lactamase and 16s rrna methylase has been reported in several countries. we analyzed p. rettgeri clinical isolates with resistance to carbapenems and aminoglycosides in a hospital in nepal.201424484534
mobile insertion cassette elements found in small non-transmissible plasmids in proteeae may explain qnrd mobilization.qnrd is a plasmid mediated quinolone resistance gene from unknown origin, recently described in enterobacteriaceae. it encodes a pentapeptide repeat protein 36-60% different from the other qnr (a, b, c, s and vc). since most qnrd-positive strains were described as strains belonging to proteus or providencia genera, we hypothesized that qnrd originated in proteeae before disseminating to other enterobacterial species. we screened 317 strains of proteeae for qnrd and its genetic support by pcr. fo ...201424504382
travel-related carbapenemase-producing gram-negative bacteria in alberta, canada: the first 3 years.we describe here the characteristics of alberta, canada, patients with infections or colonizations with carbapenemase-producing gram-negative bacteria during 2010 to 2013 that were linked to recent travel outside canada. antimicrobial susceptibility was determined by broth microdilution, and isolates were characterized using pcr, sequencing, and multilocus sequencing typing. a broth mating study was used to assess the transferability of resistance plasmids, which were subsequently characterized. ...201424599977
bacterial isolates associated with pelvic inflammatory disease among female patients attending some hospitals in abuja, nigeria.pelvic inflammatory disease refers to any infection in the female lower reproductive tract that spreads to the upper reproductive tract. the disease comprises a spectrum of inflammatory disorders of the upper female genital tract, including any combination of endometritis, salpingitis, tubo-ovarian abscess and pelvic peritonitis. pid is not a notifiable disease in most countries, so accurate statistics are not available. this situation is not in any way different here in nigeria and more so in t ...201424653811
genotype and diet shape resistance and tolerance across distinct phases of bacterial infection.host defense against pathogenic infection is composed of resistance and tolerance. resistance is the ability of the host to limit a pathogen burden, whereas tolerance is the ability to limit the deleterious effects of a given pathogen burden. this distinction recognizes that the fittest host does not necessarily have the most aggressive immune system, suggesting that host-pathogen co-evolution involves more than an escalating arms race between pathogen virulence factors and host antimicrobial ac ...201424655914
culturable aerobic and facultative anaerobic intestinal bacterial flora of black cobra (naja naja karachiensis) in southern pakistan.using morphological analysis and biochemical testing, here for the first time, we determined the culturable gut bacterial flora (aerobes and facultative anaerobes) in the venomous black cobra (naja naja karachiensis) from south asia. the findings revealed that these snakes inhabit potentially pathogenic bacteria including serratia marcescens, pseudomonas aeruginosa, shewanella putrefaciens, aeromonas hydrophila, salmonella sp., moraxella sp., bacillus sp., ochrobactrum anthropi, and providencia ...201425002979
providencia rettgeri peritonitis in a patient on peritoneal dialysis with perforated appendicitis. 201525075004
strain diversity of ctx-m-producing enterobacteriaceae in individual pigs: insights into the dynamics of shedding during the production cycle.the aim of this study was to evaluate the population dynamics of ctx-m-producing enterobacteriaceae in individual pigs on a farm positive for ctx-m-14-producing escherichia coli. fecal samples were collected once around the farrowing time from five sows and four times along the production cycle from two of their respective offspring. multiple colonies per sample were isolated on cefotaxime-supplemented macconkey agar with or without prior enrichment, resulting in 98 isolates identified by matrix ...201425128344
evaluation of a modified cefsulodin-irgasan-novobiocin agar for isolation of yersinia spp.y. enterocolitica and y. pseudotuberculosis are important food borne pathogens. however, the presence of competitive microbiota makes the isolation of y. enterocolitica and y. pseudotuberculosis from naturally contaminated foods difficult. we attempted to evaluate the performance of a modified cefsulodin-irgasan-novobiocin (cin) agar in the differentiation of y. enterocolitica from non-yersinia species, particularly the natural intestinal microbiota. the modified cin enabled the growth of y. ent ...201425170941
chemical composition and antibacterial activity of lavandula coronopifolia essential oil against antibiotic-resistant bacteria.the aim of this study was to analyse the composition of the essential oil (eo) of lavandula coronopifolia from morocco and to evaluate its in vitro antibacterial activity against antibiotic-resistant bacteria isolated from clinical infections. the antimicrobial activity was assessed by a broth micro-well dilution method using multiresistant clinical isolates of 11 pathogenic bacteria: klebsiella pneumoniae subsp. pneumoniae, klebsiella ornithinolytica, escherichia coli, enterobacter cloacae, ent ...201525174508
phenotypic and molecular characteristics of carbapenem-resistant enterobacteriaceae in a health care system in los angeles, california, from 2011 to 2013.carbapenem-resistant enterobacteriaceae (cre) are a concern for health care in the united states but remain relatively uncommon in california. we describe the phenotype, clonality, and carbapenemase-encoding genes present in cre isolated from patients at a californian tertiary health care system. cre for this study were identified by evaluating the antibiograms of enterobacteriaceae isolated in the ucla health system from 2011 to 2013 for isolates that were not susceptible to meropenem and/or im ...201425210072
first report of ndm-1-producing acinetobacter baumannii sequence type 25 in brazil.new delhi metallo-β-lactamase 1 (ndm-1) was first identified in brazil in enterobacter hormaechei and providencia rettgeri in 2013. here, we describe the first case of ndm-1-producing acinetobacter baumannii sequence type 25 isolated from the urinary tract of a 71-year-old man who died of multiple complications, including a. baumannii infection. the ndm-1 gene was detected by quantitative pcr, and its sequence confirmed its presence in an ∼ 100-kb plasmid.201425288087
nosocomial dissemination of plasmids carrying blatem-24, bladha-1, aac(6')-ib-cr, and qnra6 in providencia spp. strains isolated from a tunisian hospital.the aim of this study is to report the emergence of inca/c conjugative plasmids harboring blatem-24, bladha-1, qnra6, and aac(6')-ib-cr genes among providencia spp. isolates recovered in 2008 in tunisia. the double-disk synergy test confirmed the phenotype extended-spectrum β-lactamase (esbl) in 2 providencia stuartii and 5 providencia rettgeri. these esbls were coresistant to cefoxitin, chloramphenicol, ofloxacin, and sulfonamides but remained susceptible to imipenem. three β-lactamases tem-2, ...201525315769
antibiofilm activity of biosurfactant producing coral associated bacteria isolated from gulf of mannar.coral associated bacteria (cab) (n = 22) isolated from the mucus of the coral acropora digitifera were screened for biosurfactants using classical screening methods; hemolysis test, lipase production, oil displacement, drop collapse test and emulsifying activity. six cab (u7, u9, u10, u13, u14, and u16) were found to produce biosurfactants and were identified by 16s ribosomal rna gene sequencing as providencia rettgeri, psychrobacter sp., bacillus flexus, bacillus anthracis, psychrobacter sp., a ...201425320434
molecular and biochemical characterization of ctx-m-131, a natural asp240gly variant derived from ctx-m-2, produced by a providencia rettgeri clinical strain in são paulo, brazil.ctx-m-131 is a natural asp240gly variant from the ctx-m-2 group detected in a providencia rettgeri clinical strain from brazil. molecular analysis showed that blactx-m-131 was inserted in a complex class 1 integron harbored by a 112-kb plasmid, which has not been previously described as a platform for ctx-m-encoding genes with the asp240gly mutation. steady-state kinetic parameters showed that the enzyme has a typical cefotaximase catalytic profile and an enhanced activity against ceftazidime.201525583719
reference levels for corticosterone and immune function in farmed saltwater crocodiles (crocodylus porosus) hatchlings using current code of practice guidelines.to determine reference levels for on-farm stressors on immune responsiveness and growth rate, 253 hatchling crocodiles from 11 known breeding pairs were repeatedly measured and blood sampled during their first year. plasma corticosterone (cort) was used to quantify baseline stress levels in captive animals and were found to be lower (mean 1.83±se 0.16 ng/ml) than previously reported in saltwater crocodile hatchlings. two tests of immune function were also conducted. innate constitutive immunity ...201525644211
the complex contributions of genetics and nutrition to immunity in drosophila melanogaster.both malnutrition and undernutrition can lead to compromised immune defense in a diversity of animals, and "nutritional immunology" has been suggested as a means of understanding immunity and determining strategies for fighting infection. the genetic basis for the effects of diet on immunity, however, has been largely unknown. in the present study, we have conducted genome-wide association mapping in drosophila melanogaster to identify the genetic basis for individual variation in resistance, an ...201525764027
draft genome sequences of three ndm-1-producing enterobacteriaceae species isolated from brazil.the emergence of multidrug-resistant enterobacteriaceae strains producing carbapenemases, such as ndm-1, has become a major public health issue due to a high dissemination capacity and limited treatment options. here we describe the draft genome of three ndm-1-producing isolates: providencia rettgeri (ccbh11880), enterobacter hormaechei subsp. oharae (ccbh10892) and klebsiella pneumoniae (ccbh13327), isolated in brazil. besides blandm-1, resistance genes to aminoglycosides [aada1, aada2, aac(6') ...201526061151
replication and active partition of integrative and conjugative elements (ices) of the sxt/r391 family: the line between ices and conjugative plasmids is getting thinner.integrative and conjugative elements (ices) of the sxt/r391 family disseminate multidrug resistance among pathogenic gammaproteobacteria such as vibrio cholerae. sxt/r391 ices are mobile genetic elements that reside in the chromosome of their host and eventually self-transfer to other bacteria by conjugation. conjugative transfer of sxt/r391 ices involves a transient extrachromosomal circular plasmid-like form that is thought to be the substrate for single-stranded dna translocation to the recip ...201526061412
[emergence of providencia rettgeri ndm-1 in two departments of colombia, 2012-2013].in colombia, between 2012 and 2013, 19 isolates with ndm were identified, of which 14 corresponded to providencia rettgeri.201526130312
characterization of carbapenem-resistant gram-negative bacteria from tamil nadu.carbapenem resistance is disseminating worldwide among gram-negative bacteria. the aim of this study was to identify carbapenem-resistance level and to determine the mechanism of carbapenem resistance among clinical isolates from two centres in tamil nadu. in the present study, a total of 93 gram-negative isolates, which is found to be resistant to carbapenem by disk diffusion test in two centres, were included. all isolates are identified at species level by 16s rrna sequencing. minimal inhibit ...201626198414
biodegradation and detoxification of textile azo dyes by bacterial consortium under sequential microaerophilic/aerobic processes.release of textile azo dyes to the environment is an issue of health concern while the use of microorganisms has proved to be the best option for remediation. thus, in the present study, a bacterial consortium consisting of providencia rettgeri strain hsl1 and pseudomonas sp. suk1 has been investigated for degradation and detoxification of structurally different azo dyes. the consortium showed 98-99 % decolorization of all the selected azo dyes viz. reactive black 5 (rb 5), reactive orange 16 (r ...201526417357
first report of a ndm-producing providencia rettgeri strain in the state of são paulo. 201526432948
genomic characteristics of ndm-producing enterobacteriaceae isolates in australia and their blandm genetic contexts.blandm has been reported in different enterobacteriaceae species and on numerous plasmid replicon types (inc). plasmid replicon typing, in combination with genomic characteristics of the bacterial host (e.g., sequence typing), is used to infer the spread of antimicrobial resistance determinants between genetically unrelated bacterial hosts. the genetic context of blandm is heterogeneous. in this study, we genomically characterized 12 ndm-producing enterobacteriaceae isolated in australia between ...201526482302
optical scatter patterns facilitate rapid differentiation of enterobacteriaceae on chromagar™ orientation medium.enterobacteriaceae family comprised pathogens and commensals and has a significant impact on food safety and public health. enterobacteriaceae is often enumerated and presumptively identified on chromogenic media, such as chromagar(tm) orientation medium based on colony profile; however, classification is highly arbitrary, and some could not be differentiated due to similar chromogen production. here, we investigated the ability of the laser optical sensor, bardot (bacterial rapid detection usin ...201526503189
a case of wound infection with providencia rettgeri and coincident gout in a patient from guam.providencia rettgeri (p. rettgeri) is a ubiquitous organism that is infrequently associated with human disease. here we report the isolation of this organism from a polymicrobial wound infection resulting from ruptured tophi on a 54-year-old male patient from guam. we describe the identification and confirmation of this organism, and propose metabolic synergy as a possible mechanism of pathogenesis. to our knowledge, this is the first published report of a wound infection colonized by p. rettger ...201526568901
preparation of crosslinked enzyme aggregates (cleas) of acid urease with urethanase activity and their application.an acid urease from providencia rettgeri jn-b815 was purified via ultrasonication, ethanol precipitation, and deae ion-exchange column chromatography. it was found that the enzyme exhibits not only urease activity, but also urethanase activity, which made it possible to reduce ec already existed or would produce and its precursor urea at the same time. then, crosslinked enzyme aggregates of p. rettgeri urease (pru-cleas) were prepared using genipin as crosslinking agent. the purification process ...201626627914
bacterial volatiles attract gravid secondary screwworms (diptera: calliphoridae).bovine blood inoculated and incubated with bacteria was tested to determine if secondary screwworm, cochliomyia macellaria (f.), would be attracted to the incubated substrate for oviposition. five species of bacteria, klebsiella oxytoca (flugge), proteus mirabilis hauser, proteus vulgaris hauser, providencia rettgeri hadley, elkins and caldwell, and providencia stuartii ewing, previously isolated from animal wounds infested by primary screwworms, cochliomyia hominivorax (coquerel), were used. in ...201626748982
nitrogen removal by providencia rettgeri strain yl with heterotrophic nitrification and aerobic denitrification.providencia rettgeri strain yl shows the capability of nitrogen removal under sole aerobic conditions. by using isotope ratio mass spectrometry, (15)n-labelled n2o and n2 were detected in aerobic batch cultures containing [formula: see text], [formula: see text] or [formula: see text]. strain yl converted [formula: see text], [formula: see text] and [formula: see text] to produce more n2o than n2 in the presence of [formula: see text]. an (15)n isotope tracing experiment confirmed that the nitro ...201626824874
colistin-nonsusceptible pseudomonas aeruginosa sequence type 654 with blandm-1 arrives in north america.this study describes 3 different blandm-1 genetic platforms in 3 different species obtained from the same patient who was directly transferred to an institution in calgary, alberta, canada, following a prolonged hospital stay in india. the blandm-1 in the escherichia coli isolate was located on a 176-kb inca/c plasmid contained within an iscr1 region. the blandm-1 in the providencia rettgeri isolate was located on a 117-kb inct plasmid contained within tn3000, while the blandm-1 in the pseudomon ...201626824951
plant growth promoting potential and phylogenetic characteristics of a lichenized nitrogen fixing bacterium, enterobacter cloacae.lichens are complex symbiotic association of mycobionts, photobionts, and bacteriobionts, including chemolithotropic bacteria. in the present study, 46 lichenized bacteria were isolated by conventional and enrichment culture methods on nitrogen-free bromothymol blue (nfb) medium. only 11 of the 46 isolates fixed nitrogen on nfb and had reduced acetylene. all these 11 isolates had also produced siderophore and 10 of them the iaa. further, ammonia production was recorded from nine of these nitroge ...201627273065
genomic plasticity of multidrug-resistant ndm-1 positive clinical isolate of providencia rettgeri.we performed a detailed whole-genome sequence analysis of providencia rettgeri h1736, a multidrug-resistant clinical pathogen isolated in israel in 2011. the objective was to describe the genomic flexibility of this bacterium that has greatly contributed to its pathogenicity. the genome has a chromosome size of 4,609,352 bp with 40.22% gc content. five plasmids were predicted, as well as other mobile genetic elements (mges) including phages, genomic islands, and integrative and conjugative eleme ...201527386606
clonal transmission of gram-negative bacteria with carbapenemases ndm-1, vim-1, and oxa-23/72 in a bulgarian hospital.we characterized 72 isolates with reduced susceptibility to carbapenems (50 acinetobacter spp., 13 proteus mirabilis, five escherichia coli, one morganella morganii, one enterobacter cloacae, one providencia rettgeri, and one pseudomonas aeruginosa) from a hospital in sofia, bulgaria. different β-lactamase genes were identified by polymerase chain reaction and sequencing. bacterial strain typing was performed by enzymatic macrorestriction and pulsed-field gel electrophoresis (pfge) typing as wel ...201727459019
prevalence and antimicrobial resistance pattern of bacterial strains isolated from patients with urinary tract infection in messalata central hospital, libya.to investigate the prevalence of urinary tract infection among patients at messalata central hospital, libya, to identify the causative bacteria, and to explore their resistance pattern to antimicrobials.201627569886
ndm-producing enterobacteriaceae in a chinese hospital, 2014-2015: identification of ndm-producing citrobacterwerkmanii and acquisition of blandm-1-carrying plasmid in vivo in a clinical escherichia coli isolate.new delhi metallo-β-lactamase (ndm)-producing enterobacteriaceae (npe) shows prevalence in china. little is known about the mechanisms related to the spread of npe. recently, a total of 51 non-duplicated npe isolates were collected from a tertiary-care hospital in china and analysed for genetic relatedness by pfge, antimicrobial susceptibility by etest and sequence type by multilocus sequence typing. s1-pfge and southern blot analysis or pcr amplification were used for plasmid profiling. between ...201627667823
rna interference directed against the transglutaminase gene triggers dysbiosis of gut microbiota in drosophila.we recently reported that transglutaminase (tg) suppresses immune deficiency pathway-controlled antimicrobial peptides (imd-amps), thereby conferring immune tolerance to gut microbes, and that rnai of the tg gene in flies decreases the lifespan compared with non-tg-rnai flies. here, analysis of the bacterial composition of the drosophila gut by next-generation sequencing revealed that gut microbiota comprising one dominant genus of acetobacter in non-tg-rnai flies was shifted to that comprising ...201627760824
first case of ndm-1-producing providencia rettgeri in ecuador. 201527842879
analysis of plasmid-mediated quinolone resistance genes in clinical isolates of the tribe proteeae from argentina: first report of qnrd in the americas.to analyse the occurrence and prevalence of plasmid-mediated quinolone resistance (pmqr) genes in the tribe proteeae, 81 isolates (65 proteus spp., 12 morganella morganii and 4 providencia stuartii) consecutively collected in 66 hospitals belonging to the whonet-argentina resistance surveillance network were studied. of the 81 isolates, 50 (62%) were susceptible to quinolones [43/65 (66%) proteus spp. and 7/12 (58%) m. morganii). the remaining 31 isolates (22 proteus spp., 5 m. morganii and all ...201427873695
emergence of genetically related ndm-1-producing providencia rettgeri strains in argentina. 201427873702
identification of new bacteria harboring qnrs and aac(6')-ib/cr and mutations possibly involved in fluoroquinolone resistance in raw sewage and activated sludge samples from a full-scale wwtp.wastewater treatment plants (wwtps) harbor bacteria and antimicrobial resistance genes, favoring gene exchange events and resistance dissemination. here, a culture-based and metagenomic survey of qnra, qnrb, qnrs, and aac(6')-ib genes from raw sewage (rs) and activated sludge (as) of a full-scale municipal wwtp was performed. a total of 96 bacterial isolates were recovered from nalidixic acid-enrichment cultures. bacteria harboring the aac(6')-ib gene predominated in rs, whereas qnrs-positive is ...201727984803
the genetic architecture of defence as resistance to and tolerance of bacterial infection in drosophila melanogaster.defence against pathogenic infection can take two forms: resistance and tolerance. resistance is the ability of the host to limit a pathogen burden, whereas tolerance is the ability to limit the negative consequences of infection at a given level of infection intensity. evolutionarily, a tolerance strategy that is independent of resistance could allow the host to avoid mounting a costly immune response and, theoretically, to avoid a co-evolutionary arms race between pathogen virulence and host r ...201728099780
first complete providencia rettgeri genome sequence, the ndm-1-producing clinical strain rb151.providencia rettgeri is an opportunistic bacterial pathogen of clinical significance due to its association with urinary tract infections and multidrug resistance. here, we report the first complete genome sequence of p. rettgeri the genome of strain rb151 consists of a 4.8-mbp chromosome and a 108-kbp blandm-1-positive plasmid.201728104655
outbreak of carbapenem-resistant providencia rettgeri in a tertiary hospital.the emergence of resistance to multiple antimicrobial agents in pathogenic bacteria is a significant public health threat, as there are limited effective antimicrobial agents for infections caused by multidrug-resistant (mdr) bacteria. several mdr bacteria are now frequently detected. carbapenem resistance in enterobacteriaceae is often plasmid mediated, necessitating stringent infection control practices. wedescribe an outbreak of carbapenem-resistant providencia rettgeri involving 4 patients a ...201628112086
detection of sgi1/pgi1 elements and resistance to extended-spectrum cephalosporins in proteae of animal origin in france.proteae, and especially proteus mirabilis, are often the cause of urinary tract infections (utis) in humans. they were reported as carriers of extended-spectrum β-lactamase (esbl) genes, and recently of carbapenemases, mostly carried by the salmonella genomic island 1 (sgi1) and proteus genomic island 1 (pgi1). proteae have also lately become an increasing cause of utis in companion animals, but antimicrobial susceptibility data in animals are still scarce. here, we report the characterization o ...201728154560
expression of an acid urease with urethanase activity in e. coli and analysis of urease gene.urea in alcoholic beverage is a precursor of ethyl carbamate (ec), which is carcinogenic. enzymatic elimination of urea has attracted much research interest. acid urease with good tolerance toward ethanol and acid is ideal enzyme for such applications. in the present work, the structural genes of urease from providencia rettgeri jn-b815, ureabc were efficiently expressed in e. coli bl21(de3) in an active form (apourease) exhibiting both urease and urethanase (hydrolyze ec) activities. the specif ...201728197768
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