Publications
Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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peptide hydrolase activity of klebsiella aerogenes. | 1969 | 5767044 | |
effect of growth rate on histidine catabolism and histidase synthesis in aerobacter aerogenes. | a study was made of how the catabolism of a carbon and energy source is affected by the biosynthetic demands of growing bacterial cells. cultures of aerobacter aerogenes in l-histidine medium were grown in a chemostat at rates determined by the supply of either sulfate or a required amino acid, l-arginine. it was discovered that the rate at which these cells grow under a biosynthetic restriction determines both the rate and the pattern of histidine degradation. (i) histidine catabolism is partia ... | 1969 | 5781570 |
regulation of gratuitous beta-galactosidase synthesis in aerobacter aerogenes during an adaptive process. | 1969 | 5783886 | |
theory of oscillations of respiration rate in continuous culture of klebsiella aerogenes. | 1969 | 5783912 | |
requirement for sodium in the anaerobic growth of aerobacter aerogenes on citrate. | anaerobic growth of aerobacter aerogenes on citrate as a carbon source required the presence of na(+). the growth rate increased with increasing na(+) concentration and was optimal at 0.10 m na(+). the requirement was specific for na(+), which could not be replaced by k(+), nh(4) (+), li(+), rb(+), or cs(+). k(+) was required for growth in the presence of na(+), the optimal k(+) concentration being 0.15 mm. enzyme profiles were determined on cells grown in three different media: (i) intermediate ... | 1969 | 5784198 |
induction and properties of the citrate transport system in aerobacter aerogenes. | the mediated transport of citrate in aerobacter aerogenes was studied. according to data obtained by examining the distribution of radioactive citrate at room temperature and at 0 c, a carrier system appears to be located on the membrane. the carrier system is inducible and very specific, not acting on the related compounds isocitrate and cis-aconitate. induction required synthesis of both ribonucleic acid and protein as determined by starving auxotrophic mutants and by using specific inhibitors ... | 1969 | 5784212 |
role of sodium in determining alternate pathways of aerobic citrate catabolism in aerobacter aerogenes. | in contrast to the absolute na(+) requirement for anaerobic growth of aerobacter aerogenes on citrate as sole carbon source, aerobic growth of this microorganism did not require the presence of na(+). however, na(+) (optimal concentration, 10 mm) did increase the maximal amount of aerobic growth by 60%, even though it did not change the rate of growth. this increase in growth was specifically affected by na(+), which could not be replaced by k(+), nh(4) (+), li(+), rb(+), or cs(+). enzyme profil ... | 1969 | 5808070 |
bactericidal activity of normal milk, mastitic milk, and colostrum against aerobacter aerogenes. | 1969 | 5815918 | |
the ph 6 acetolactate-forming enzyme from aerobacter aerogenes. determination and function of thiol groups. | 1969 | 5823100 | |
the ph 6 acetolactate-forming enzyme from aerobacter aerogenes. molecular properties. | 1969 | 5823101 | |
immunochemical studies on some serological cross-reactions in the klebsiella group. 13. serological investigation of the cross-reaction of klebsiella type 3(c), klebsiella aerogenes strain b.1076/48 and "enterobacter" strain 349. | 1965 | 5829964 | |
repression and induction of arylsulphatase synthesis in aerobacter aerogenes. | 1964 | 5838663 | |
n-formimino-l-glutamate formiminohydrolase of aerobacter aerogenes. | 1965 | 5845833 | |
induction and repression of the histidine-degrading enzymes in aerobacter aerogenes. | 1965 | 5845834 | |
imidazolepropionate, a nonmetabolizable inducer for the histidine-degrading enzymes in aerobacter aerogenes. | 1965 | 5845835 | |
exogenous and endogenous induction of the histidine-degrading enzymes in aerobacter aerogenes. | 1965 | 5845836 | |
growth of aerobacter aerogenes on d-arabinose and l-xylose. | 1965 | 5847804 | |
the use of a synthetic corticoid on experimental coliform (aerobacter aerogenes) mastitis in cattle: the effects of intramammary and intramuscular administration on the inflammatory response. | 1965 | 5857177 | |
the gel-filtration behaviour of proteins related to their molecular weights over a wide range. | 1. correlation between elution volume, v(e), and molecular weight was investigated for gel filtration of proteins of molecular weights ranging from 3500 (glucagon) to 820000 (alpha-crystallin) on sephadex g-200 columns at ph7.5. 2. allowing for uncertainties in the molecular weights, the results for most of the carbohydrate-free globular proteins fitted a smooth v(e)-log(mol.wt.) curve. in the lower part of the molecular-weight range the results were similar to those obtained with sephadex g-75 ... | 1965 | 5862401 |
magnesium-limited growth of aerobacter aerogenes in a chemostat. | 1965 | 5864530 | |
[cytological study, growth curve and glucose consumption of irradiated aerobacter aerogenes]. | 1965 | 5865558 | |
antimicrobial activity of several bis-methanethiolsulfonates. | we determined the inhibitory end point of a series of bis-methanethiolsulfonates, ch(3)so(2)s(ch(2))(n)sso(2)ch(3) (n = 2 to 6), and correlated the structures with antimicrobial activity. eleven microorganisms were used in the evaluation, and the maximal activity occurred when the methylene chain length was five or six. the effect of ch(3)so(2)s(ch(2))(5)sso(2)ch(3) (pmts) on respiration of aerobacter aerogenes and staphylococcus aureus was studied at various stages of their growth cycles. the e ... | 1965 | 5866041 |
the influence of temperature and ph value on the macro-molecular composition of magnesium-limited and glycerol-limited aerobacter aerogenes growing in a chemostat. | 1965 | 5866131 | |
[excretion of methionine and ethionine by enterobacter aerogenes]. | 1965 | 5877968 | |
the ribosephosphate and glucosephosphate isomerases of aerobacter aerogenes. | 1965 | 5879507 | |
enzymic conversion of glycerol into beta-hydroxy-propionaldehyde in a cell-free extract from aerobacter aerogenes. | 1965 | 5882686 | |
methyl sulfide production by aerobacter aerogenes in milk. | 1965 | 5897822 | |
the pathway of myo-inositol degradation in aerobacter aerogenes. dehydrogenation and dehydration. | 1966 | 5905122 | |
the pathway of myo-inositol degradation in aerobacter aerogenes. ring scission. | 1966 | 5905123 | |
stereospecific d-glucokinase of aerobacter aerogenes. purification and properties. | 1966 | 5908135 | |
the composition of lipopolysaccharides of klebsiella aerogenes and aerobacter cloacae. | 1966 | 5914339 | |
effect of amino acids on the nitrogenase system of klebsiella pneumoniae. | yoch, d. c. (south dakota state university, brookings), and r. m. pengra. effect of amino acids on the nitrogenase system of klebsiella pneumoniae. j. bacteriol. 92:618-622. 1966.-the effect of exogenous amino acids and the free amino acid pool on the synthesis of the nitrogenase system of klebsiella pneumoniae m5al (formerly aerobacter aerogenes m5al) was investigated. when an actively n(2)-fixing culture was used to inoculate a medium containing a limiting concentration of nh(4) (+), an induct ... | 1966 | 5922536 |
diaphorases from aerobacter aerogenes. | bernofsky, carl (the university of kansas, kansas city), and russell c. mills. diaphorases from aerobacter aerogenes. j. bacteriol. 92:1404-1414. 1966.-five enzymes which catalyze the reduction of 2,6-dichlorophenol-indophenol by reduced nicotinamide adenine dinucleotide (nadh(2)) have been separated from sonic extracts of aerobacter aerogenes b199 by diethylaminoethyl (deae) cellulose chromatography. three major chromatographic fractions (enzymes i, ii, and iii) account for most of the activity ... | 1966 | 5924271 |
ultracentrifugal studies of the variation with physiological state of the ribosomes in aerobacter aerogenes. | 1966 | 5928603 | |
the purification and properties of d-allosephosphate isomerase of aerobacter aerogenes. | 1966 | 5929157 | |
pullulanase from aerobacter aerogenes; production in a cell-bound state. purification and properties of the enzyme. | 1966 | 5938919 | |
a clinical trial of cephaloridine. | cephaloridine, a cephalosporin derivative, was administered to 30 selected patients, including 19 with moderate to severe impairment of renal function. this antibiotic eradicated infections due to staphylococcus pyogenes, and urinary tract infections due to a single member of the species escherichia coli or aerobacter aerogenes, which were sensitive to the drug on bacteriological testing. the drug failed in mixed urinary tract infections.no adverse effects were observed except for the developmen ... | 1966 | 5940638 |
[contribution to the study of the pyruvate-reductase of aerobacter aerogenes]. | 1966 | 5942655 | |
the structure of the aerobacter aerogenes a3(s1) polysaccharide. i. a reexamination using improved procedures for methylation analysis. | 1966 | 5961274 | |
the structure of the aerobacter aerogenes a3(s1) polysaccharide. ii. sequence analysis and hydrolysis studies. | 1966 | 5961866 | |
the properties of glycerol dehydratase isolated from aerobacter aerogenes, and the properties of the apoenzyme subunits. | 1966 | 5962440 | |
pneumohepatitis caused by klebsiella aerogenes. | 1966 | 5963343 | |
variation in content and distribution of magnesium, and its influence on survival, in aerobacter aerogenes grown in a chemostat. | 1966 | 5969503 | |
the adaptation of aerobacter aerogenes to the stress of sublethal doses of formaldehyde. | 1966 | 5969748 | |
role of lipid in the protection of staphylococcus aureus against trichlorophenol in mixed culture. | the sensitivities of proteus mirabilis, salmonella schottmuelleri, aerobacter aerogenes, and staphylococcus aureus to 2, 4, 6-trichlorophenol in sodium borate were studied. it was demonstrated that these gram-negative organisms can protect s. aureus from the effect of the phenol in mixed culture. there is a direct correlation between this protective effect and the quantity of total lipid extracted from the gram-negative organisms. the distribution coefficient between trichlorophenol and the lipi ... | 1966 | 5970464 |
[a case of septicemia caused by aerobacter aerogenes]. | 1966 | 5977185 | |
magnesium starvation of aerobacter aerogenes. i. changes in nucleic acid composition. | aerobacter aerogenes incubated in a medium containing all factors necessary for exponential growth except mg(++) continued to synthesize nucleic acids and proteins for more than 70 hr, provided the major carbon source was in excess at all times. after 24 hr of mg(++) starvation, deoxyribonucleic acid content in the culture had increased 10-fold. in contrast, the viable-cell count increased only about threefold during the first few hours and then remained approximately constant for the subsequent ... | 1967 | 6020411 |
magnesium starvation of aerobacter aerogenes. 3. protein metabolism. | the metabolism of the ribosomal and soluble protein components of aerobacter aerogenes was examined during its incubation in a mg(++)-deficient medium. bacteria were exposed to leucine-h(3) during the exponential growth period preceding mg(++) starvation, and extracts were prepared after intervals of starvation and were centrifuged through gradients of sucrose to separate ribosomal from soluble proteins. ribosomal proteins synthesized during the preceding exponential growth were slowly lost from ... | 1967 | 6020412 |
magnesium starvation of aerobacter aerogenes. ii. rates of nucleic acid synthesis and methods for their measurement. | the rates of synthesis of aerobacter aerogenes nucleic acids were estimated during incubation of the bacteria in a mg(++)-free medium. deoxyribonucleic acid (dna) synthesized during mg(++) starvation, or in the preceding exponential growth, remained acid-precipitable for 2.5 hr before breaking down to acid-soluble products during a period of many hours. rates of dna synthesis were calculated by correcting the net amounts of dna per milliliter to values that would have appeared had there been no ... | 1967 | 6021070 |
isolation of crystalline ph 6 acetolactate-forming enzyme from aerobacter aerogenes. | 1967 | 6024768 | |
metabolic injury to bacteria. ii. metabolic injury induced by distilled water or cu++ in the plating diluent. | when distilled water from a tin-lined still served as the plating diluent, cells of aerobacter aerogenes developed symptoms of metabolic injury as evidenced by increased counts on supplemented, as compared with minimal, plating medium. cysteine was as effective as yeast extract as a supplement to the minimal medium in increasing the viable count. mg(++) and, to a lesser extent, phosphate buffer at the concentrations tested protected unfrozen cells, but not cells which had been frozen and stored, ... | 1967 | 6025433 |
influence of growing aerobacter aerogenes in a polyamine-enriched medium on the stability of the 70s ribosomes. | 1967 | 6028139 | |
the influence of dissolved oxygen concentration on the respiration and glucose metabolism of klebsiella aerogenes during growth. | 1967 | 6029730 | |
kinetic aspects of the growth of klebsiella aerogenes with some benzenoid carbon sources. | 1967 | 6029731 | |
potassium-ammonium antagonism in polysaccharide synthesis by aerobacter aerogenes nctc 418. | 1967 | 6032041 | |
effects of nitrogen supplements on nitrogen fixation by aerobacter aerogenes. | 1967 | 6032045 | |
inhibition of shigella flexneri by the normal intestinal flora. i. mechanisms of inhibition by klebsiella. | growth curves were plotted for shigella flexneri and klebsiella (aerobacter aerogenes) multiplying in pure and mixed culture. in mixed culture, klebsiella inhibited shigella. exponential growth was interrupted and shigella entered into a logarithmic death phase. an analysis of cultures at the time inhibition occurred revealed that formic and acetic acids produced by klebsiella were responsible for the inhibition of shigella. klebsiella strongly reduced the culture medium. the volatile fatty acid ... | 1967 | 6032512 |
acyl phosphate: hexose phosphotransferase. purification and properties of the enzyme from aerobacter aerogenes and evidence for its common identity with hexose phosphate: hexose phosphotransferase. | 1967 | 6033450 | |
bactericidal activity for aerobacter aerogenes of bovine serum and cell-free normal and mastitic milks. | 1967 | 6033692 | |
phagocytosis and destruction of aerobacter aerogenes by leukocytes from bovine milk. | 1967 | 6033693 | |
further evidence for two distinct acetolactate synthetases in aerobacter aerogenes. | 1967 | 6034688 | |
dechlorination of 1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane by aerobacter aerogenes. i. metabolic products. | whole cells or cell-free extracts of aerobacter aerogenes catalyze the degradation of 1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane (ddt) in vitro to at least seven metabolites: 1,1-dichloro-2,2-bis(p-chlorophenyl)ethylene (dde); 1,1-dichloro-2,2-bis(p-chlorophenyl)ethane (ddd); 1-chloro-2,2-bis(p-chlorophenyl)ethylene (ddmu); 1-chloro-2,2-bis(p-chlorophenyl)ethane (ddms); unsym-bis(p-chlorophenyl)ethylene (ddnu); 2,2-bis(p-chlorophenyl)acetate (dda); and 4,4'-dichlorobenzophenone (dbp). the use ... | 1967 | 6035049 |
phage-induced fucosidases hydrolysing the exopolysaccharide of klebsiella arogenes type 54 [a3(s1)]. | several strains of bacteriophage have been isolated that induce the formation of a polysaccharide hydrolase after infection of klebsiella aerogenes type 54 [a3(s1)]. the action of this enzyme on polysaccharide solutions was to decrease their viscosity and increase their reducing value. these effects were associated with the release of two oligosaccharides (o1 and o2) from the polysaccharide. these two substances are not identical with any of the four oligosaccharides isolated from autohydrolysat ... | 1967 | 6035518 |
enzymatic determination of vitamin b12, coenzyme b12, and other cobamide derivatives in picomole quantities by means of glycerol dehydratase from aerobacter aerogenes. | 1967 | 6044270 | |
the production of an n-acylanthranilic acid from shikimic acid and the effect on iron deficiency on the biosynthesis of other aromatic compounds by aerobacter aerogenes. | 1967 | 6051584 | |
gentamicin sulfate in the treatment of skin infection due to aerobacter aerogenes. | 1967 | 6063497 | |
the distribution of aerobacter aerogenes in the urinary tract of guinea pigs following ascending routes of inoculation. | 1967 | 6068995 | |
effects of humoral and cellular phases of acute inflammation in the bovine mammary gland on aerobacter aerogenes introduced en masse and on leukocytes existing in milk. | 1967 | 6069569 | |
the role of primer in glycogen biosynthesis in aerobacter aerogenes. | 1967 | 6076622 | |
regulation of hutuh operon expression by the catabolite gene activator protein-cyclic amp complex in klebsiella aerogenes. | rna polymerase transcribed the hutuh operon of klebsiella aerogenes if the catabolite gene activator protein (cap) and cyclic amp (camp) were present or if the dna template was derived from a promoter mutant in which hutuh expression was independent of the need for positive effectors. in the absence of cap or camp, not only was hutuh transcription absent, but transcription in the opposite direction (toward hutc) was initiated at a site (pc) ca. 70 base pairs from the site (puh) of hutuh mrna ini ... | 1984 | 6090399 |
[clinical laboratory approach for estimating effective administrative dosage of cefmenoxime. observation on the mics and cefmenoxime disc susceptibility test]. | the in vitro activity of cefmenoxime (cmx) was determined using agar dilution at inoculum level of 10(6) cfu/ml against 333 clinical bacterial isolates. cmx was highly active against escherichia coli, klebsiella pneumoniae, proteus mirabilis, enterobacter aerogenes and haemophilus influenzae and also streptococcus pyogenes and streptococcus pneumoniae with mic values in the range of 0.024 to 3.13 micrograms/ml. against staphylococci and serratia marcescens, cmx showed the antimicrobial activity ... | 1984 | 6090721 |
in vitro activity and beta-lactamase stability of cefmenoxime. | the activity of cefmenoxime, an aminothiazolyl cephalosporin, was studied against 650 bacteria. it was slightly less active than cefotaxime and more active than moxalactam against staphylococci. it had activity similar to that of cefotaxime and ceftizoxime against group a and b streptococci and streptococcus pneumoniae. it did not inhibit streptococcus faecalis or listeria spp. cefmenoxime had activity similar to that of cefotaxime, ceftizoxime, ceftazidime, and moxalactam against escherichia co ... | 1982 | 6100427 |
isolation of klebsiella aerogenes mutants cis-dominant for glutamine synthetase expression. | we have isolated three strains of klebsiella aerogenes that failed to show repression of glutamine synthetase even when grown under the most repressing conditions for the wild-type strain. these mutant strains were selected as glutamine-independent derivatives of a strain that is merodiploid for the glna region and contains a mutated glnf allele. the mutation responsible for the gln+ phenotype in each strain was tightly linked to glna, the structural gene for glutamine synthetase, and was domina ... | 1980 | 6102550 |
purification of glutamine synthetase from a variety of bacteria. | we have developed two procedures which allow the very rapid purification of glutamine synthetase (gs) from a diverse variety of bacteria. the first procedure, based upon differential sedimentation, depends upon the association of gs with deoxyribonucleic acid in cell extracts. the second procedure, derived from the method of c. gross et al (j. bacteriol. 128:382-389, 1976) for purifying ribonucleic acid polymerase by polyethylene glycol (peg) precipitation, enabled us to obtain high yields of gs ... | 1980 | 6102984 |
[interest of gamma-glutamyltransferase in "enterobacteriaceae" (author's transl)]. | gamma-glutamyltransferase (gammagt) could be detected in 86,6% of 3,027 strains of enterobacteriaceae, by the use of gamma-l-glutamin-p-nitranilide acid for substrate. the following species produced gamma gt: citrobacter freundii, levinea malonatica, l. amalonatica, klebsiella pneumoniae, k. oxytoca, k. ozaenae, enterobacter aerogenes, e. cloacae, e. agglomerans, e. gergoviae, k. ozaenae, enterobacter aerogenes, e. cloacae, e. agglomerans, e. gergoviae, hafnia alvei, erwinia carotovora, serratia ... | 1980 | 6104464 |
regulation of the synthesis of glutamine synthetase by the pii protein in klebsiella aerogenes. | certain mutations at the glab locus result in the failure to fully derepress glutamine synthetase [l-glutamate:ammonia ligase (adp-forming), ec 6.3.1.2] and to convert it to the active nonadenylylated form in response to nitrogen limitation. in these mutants the pii regulatory protein is altered such that it cannot be converted by uridylyltransferase to the form stimulating deadenylylation of glutamine synthetase by adenylyltransferase. additional mutations as well as insertions of transposon tn ... | 1980 | 6104810 |
tryptophan metabolism in klebsiella aerogenes: regulation of the utilization of aromatic amino acids as sources of nitrogen. | klebsiella aerogenes utilized aromatic amino acids as sole sources of nitrogen but not as sole sources of carbon. k. aerogenes abstracted the alpha-amino group of these compounds by transamination and excreted the arylpyruvate portions into the medium. when tryptophan was utilized as the sole source of nitrogen by k. aerogenes, indolepyruvate was excreted into the medium, where it polymerized non-enzymatically to form a brick red pigment. at least four separate aromatic aminotransferase activiti ... | 1981 | 6109705 |
regulation of glutamine synthetase by regulatory protein pii in klebsiella aerogenes mutants lacking adenylyltransferase. | a mutation of klebsiella aerogenes causing production of an altered pii regulatory protein which stimulates overadenylylation of glutamine synthetase and also prevents its derepression was combined with mutations abolishing the activity of adenylyltransferase. the results support the idea that pii plays a role in the regulation of the level of glutamine synthetase which is independent of its interaction with adenylyltransferase. | 1981 | 6111558 |
role of glna-linked genes in regulation of glutamine synthetase and histidase formation in klebsiella aerogenes. | we isolated mutants of klebsiella aerogenes with insertions of transposon tn5 linked to the structural gene for glutamine synthetase, glna. we found that k. aerogenes, like escherichia coli and salmonella typhimurium, contains a regulatory gene, glng, which is closely linked to but distinct from glna. the product of glng is essential for normal regulation of the synthesis of glutamine synthetase and histidase. we analyzed two mutations which affected the regulation of the formation of these enzy ... | 1982 | 6120931 |
effects of glnl and other regulatory loci on regulation of transcription of glna-lacz fusions in klebsiella aerogenes. | mutants of klebsiella aerogenes containing genetic fusions of glna to lacz were isolated by using mu dl (lac, bla) bacteriophage and a mu kmr helper phage with the host range of bacteriophage p1. synthesis of beta-galactosidase in these strains is regulated in response to nitrogen metabolites and regulatory gln loci and is rendered constitutive by a mutation in the linked glnl gene. complementation studies indicated that glnl is a separate locus from glna and glng and that insertions in glna are ... | 1982 | 6120932 |
asparagine synthetases of klebsiella aerogenes: properties and regulation of synthesis. | we isolated pleiotropic mutants of klebsiella aerogenes with the transposon tn5 which were unable to utilize a variety of poor sources of nitrogen. the mutation responsible was shown to be in the asnb gene, one of two genes coding for an asparagine synthetase. mutations in both asna and asnb were necessary to produce an asparagine requirement. assays which could distinguish the two asparagine synthetase activities were developed in strains missing a high-affinity asparaginase. the asna and asnb ... | 1982 | 6125499 |
a nonsense mutation in the structural gene for glutamine synthetase leading to loss of nitrogen regulation in klebsiella aerogenes. | an amber mutation (glna3711), the first nonsense mutation isolated in klebsiella aerogenes, is described. when amber suppressors were present, the mutant made active glutamine synthetase which was more thermolabile than wild type, showing that glna3711 lies in the structural gene for glutamine synthetase. strains carrying the glna3711 allele were unable to express nitrogen regulation of genes coding for histidase, asparaginase, and glutamate dehydrogenase unless amber suppressors were also prese ... | 1982 | 6129565 |
identification of major sulfate conjugates in the metabolism of propranolol in dog and man. | a large portion of the dose of propranolol in animals and man is unaccounted for. using radiotracer and hplc techniques, five previously unrecognized polar and labile metabolites were found in dog urine, together accounting for 34% of the urinary radioactivity. the two main metabolites, peak 2 (7% of the radioactivity) and peak 4 (17%) could be isolated and purified by butanol extraction and reversed phase hplc. direct probe ms analysis of the main peak 4 and gc/ms analysis of the same peak afte ... | 1983 | 6137341 |
[clinical problems of surgical infection. the pirogov institute for emergency medicine, sofia]. | the aetiology, pathogenesis, diagnosis and therapy of sepsis are dealt with in this paper. these problems are discussed on the basis of 151 patients treated for sepsis. the cases with monoinfection are 73.6% and those with polyinfection are 24.4%. monoinfection is caused mainly by staphylococci -65,3%, followed by e. coli - 15.2%, proteus - 13% and klebsiella - 3.3%. for the cases of polyinfection the gram-negative bacteria are 3:1 in respect to the gram-positive bacteria. the bacteriological fi ... | 1984 | 6150608 |
substrate specificity of citrate lyase deacetylase of rhodopseudomonas gelatinosa and rhodopseudomonas palustris. | citrate lyase (ec 4.1.3.6) isolated from rhodopseudomonas palustris was investigated with regard to its kinetic properties and its subunit composition. this enzyme was inactivated by citrate lyase deacetylase (ec 3.1.2.-) of rhodopseudomonas gelatinosa. a corresponding cross-reaction was measured with partially purified deacetylase of r. palustris and citrate lyase of r. gelatinosa. the three different subunit types (alpha, beta, and gamma) of citrate lyase from r. gelatinosa wee purified to hom ... | 1981 | 6167565 |
pausing of rna polymerase during in vitro transcription of the tryptophan operon leader region. | rna polymerase molecules pause at a single site during in vitro transcription of the tryptophan (trp) operon leader region. pausing was observed when dna templates derived from escherichia coli. salmonella typhimurium, and klebsiella aerogenes were used. fingerprint analyses showed that the major rna species produced by the transcriptional pause is 91 nucleotides long. a minor rna species 90 nucleotides long was also detected. single-round transcription experiments were used to study the kinetic ... | 1981 | 6168281 |
ribitol dehydrogenase messenger rna from an enzyme superproducer strain of klebsiella aerogenes. purification, cell-free translation and studies in vitro and in vivo. | 1. ribitol dehydrogenase messenger rna, from a strain of klebsiella aerogenes that had been evolved to superproduce this enzyme, has been purified in a single step by labelling extracted polysomes with rabbit anti(ribitol dehydrogenase) and immunoprecipitating with sheep anti-(rabbit igg). 2. the extracted mrna is stable in a protein synthesis system in vitro and directs synthesis 35-40-times more efficiently than rna from coliphages ms2 or q beta, to give ribitol dehydrogenase as sole major pro ... | 1982 | 6174332 |
regulatory region of the klebsiella aerogenes tryptophan operon. | the trp operon of klebsiella aerogenes was cloned, and its regulatory region was sequenced. comparison with previously reported trp regulatory sequences of other enteric bacteria indicates that the k. aerogenes trp promoter-operator region is most similar to the corresponding region of salmonella typhimurium. the trp leader regions of k. aerogenes and other enteric bacteria are organized similarly, but there are significant differences in the stabilities of the predicted secondary structures in ... | 1982 | 6181051 |
suction apparatus and hospital infection due to multiply-resistant klebsiella aerogenes. | 1982 | 6181135 | |
the role of the o and k antigens in determining the resistance of klebsiella aerogenes to serum killing and phagocytosis. | the presence of both k and o antigens of klebsiella aerogenes was found necessary to protect the organism from either complement-mediated serum killing or phagocytosis in the absence of specific antisera. optimal phagocytic ingestion of k. aerogenes nctc 5055 could be achieved in the presence of either anti-k or anti-o sera or to a much smaller extent in antisera raised against a rough unencapsulated mutant (m10b) derived from nctc 5055. anti-o sera failed to opsonize a clinical klebsiella isola ... | 1983 | 6195306 |
survival of multiply-resistant klebsiella aerogenes and other gram-negative bacilli on finger-tips. | the survival of various gram-negative bacilli was evaluated by inoculating the finger-tips of volunteers and determining the number of recoverable organisms in finger washings taken at increasing time intervals up to 60 min. three epidemic gentamicin-resistant multiply-resistant strains of klebsiella aerogenes (capsular types k2, k16 and k21) survived better than pseudomonas aeruginosa, escherichia coli or non-epidemic antibiotic sensitive klebsiellae of corresponding capsular type. the survival ... | 1983 | 6198364 |
clinical and pharmacokinetic evaluation of parenteral moxalactam in infants and children. | thirty-four infants and children ranging in age from 2.5 to 180 months (mean, 40 months) were treated with parenteral moxalactam (150 mg/kg per day) for suspected or proved bacterial infections outside the central nervous system. six patients infected with haemophilus influenzae b, nine infected with staphylococcus aureus, three infected with streptococcus pneumoniae, one infected with streptococcus pyogenes, one infected with enterobacter aerogenes, one infected with fusobacterium nucleotum, an ... | 1982 | 6214210 |
in vitro activity of norfloxacin, a quinolinecarboxylic acid, compared with that of beta-lactams, aminoglycosides, and trimethoprim. | norfloxacin is a quinolinecarboxylic acid compound. we examined the in vitro activity of this compound against gram-positive and -negative species, including anaerobic species. it inhibited 90% (mic90) of strains of escherichia coli at 0.05 microgram/ml, klebsiella sp. at 0.4 microgram/ml, salmonella and shigella spp. at 0.1 microgram/ml, citrobacter sp. at 0.4 microgram/ml, enterobacter cloacae at 0.2 microgram/ml, enterobacter aerogenes at 0.4 microgram/ml, and enterobacter agglomerans at 0.2 ... | 1982 | 6214995 |
microcalorimetric studies of klebsiella aerogenes grown in chemostat culture. 3 transient (non-steady) state. | the increased power output resulting from the addition of small amounts of different substrates to glucose-limited chemostats depended on the added c-source; four types of substrate were recognised. the additional heat evolved increased linearly with the amount of added acetate, but not with glucose or pyruvate. small amounts of uncouplers disturbed the steady-state power output, and the increased heat was related to the stimulation of the atpase system. the enhanced power output on increasing t ... | 1982 | 6216394 |
in vitro activity of moxalactam against pathogenic bacteria and its comparison with other antibiotics. | 843 isolates from clinical specimens were tested against moxalactam by disc agar diffusion. the bacteria used in this study consisted of escherichia coli, enterobacter aerogenes, enterobacter agglomerans, enterobacter cloacae, klebsiella pneumoniae, proteus mirabilis, providencia rettgeri, pseudomonas aeruginosa, serratia marcescens, staphylococcus aureus, staphylococcus epidermidis and group b and group d streptococci. in vitro activity of moxalactam was compared with the following antibiotics: ... | 1983 | 6219865 |
studies on the protease inhibitor of klebsiella aerogenes. | 1983 | 6234774 | |
effect of iron deprivation on the production of siderophores and outer membrane proteins in klebsiella aerogenes. | the outer membrane (om) protein profile of klebsiella aerogenes grown in an iron rich chemically defined medium (fe + cdm) showed three major proteins of 32.5, 35.5 and 39.0 kdal. the 35.5 and 39.0 kdal proteins were non-covalently associated with peptidoglycan. at least six new iron regulated outer membrane proteins (irmp) of 69, 70, 73, 75, 78 and 83 kdal, which were not peptidoglycan associated, were apparent in the om of k. aerogenes grown in iron restricted (serum) or iron deficient (fe-cdm ... | 1984 | 6239009 |
in vitro activity of pefloxacin compared to that of quinolones and other antimicrobial agents. | pefloxacin is a new methyl-4-piperazinyl quinolone. it had mic90 values of less than 0.01 to 0.8 micrograms/ml for the majority of escherichia coli, klebsiella, oxytoca, citrobacter, providencia, enterobacter cloacae, enterobacter aerogenes, morganella and proteus mirabilis. it inhibited ampicillin, cephalexin and nalidixic acid resistant isolates of these species. against pseudomonas the pefloxacin mic90 was 3.1 micrograms/ml. staphylococcus aureus had a mic50 of 0.4 micrograms/ml and a mic90 o ... | 1984 | 6241847 |
genetic control of tyramine oxidase, which is involved in derepressed synthesis of arylsulfatase in klebsiella aerogenes. | mutants of klebsiella aerogenes with three types of mutations affecting regulation of tyramine oxidase were isolated by a simple selection method. in the first type, the mutation (tynp) was closely linked to the structural gene for tyramine oxidase tyna). the order of mutation sites was atsa-tynp-tyna. in the second type, the mutation that relieves catabolite repression of the syntheses of several catabolite repression-sensitive enzymes are not linked to the tyn gene by p1 transduction. these st ... | 1980 | 6249789 |
physical maps of klebsiella aerogenes and salmonella typhimurium hut genes. | the recognition sites for several restriction endonucleases were mapped within deoxyribonucleic acid coding for histidine utilization (hut) genes of salmonella typhimurium and klebsiella aerogenes. deoxyribonucleic acid fragments containing the two hut promoters were identified by ribonucleic acid polymerase binding. | 1981 | 6257644 |