Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| community acquired bacteremia by sphingomonas paucimobilis: two rare case reports. | s.paucimobilis has a diverse nutritional substrate spectrum and found in both environmental and hospital settings. sphingomonas paucimobilis is rarely isolated from clinical specimen. this low virulence organism since has been reported to cause a variety of diseases since 1979. it has been reported to be associated with both community acquired and nosocomial diseases including bacteremia, catheter related sepsis, diarrhoeal diseases, peritonitis, meningitis, cutaneous infections, endopthalmitis, ... | 2013 | 24551684 |
| postoperative endophthalmitis following cataract surgery in asia. | postoperative endophthalmitis is a rare clinical occurrence. however, it remains one of the most serious complications following cataract surgery because of its poor prognosis. we investigated the epidemiologic trends in postoperative endophthalmitis following cataract surgery, particularly in asian populations. the incidence of postcataract endophthalmitis was generally consistent with epidemiologic data reported from caucasian populations. the most frequently occurring causative organism was c ... | 2011 | 24555121 |
| structural characterization of a hypothetical protein: a potential agent involved in trimethylamine metabolism in catenulispora acidiphila. | catenulispora acidiphila is a newly identified lineage of actinomycetes that produces antimicrobial activities and represents a promising source of novel antibiotics and secondary metabolites. among the discovered protein coding genes, 68 % were assigned a putative function, while the remaining 32 % are genes encoding "hypothetical" proteins. caci_0382 is one of the "hypothetical" proteins that has very few homologs. sequence analysis shows that the protein belongs to the ntf2-like protein famil ... | 2014 | 24562475 |
| cloning and characterisation of (r)-3-hydroxyacyl-acyl carrier protein-coenzyme a transferase gene (phag) from pseudomonas sp. usm 4-55. | the (r)-3-hydroxyacyl-acp-coa transferase catalyses the conversion of (r)-3-hydroxyacyl-acp to (r)-3-hydroxyacyl-coa derivatives, which serves as the ultimate precursor for polyhydroxyalkanoate (pha) polymerisation from unrelated substrates in pseudomonads. phag was found to be responsible for channelling precursors for polyhydroxyalkanoate (pha) synthase from a de novo fatty acid biosynthesis pathway when cultured on carbohydrates, such as glucose or gluconate. the phag gene was cloned from pse ... | 2009 | 24575175 |
| bacterial degradation of chlorophenols and their derivatives. | chlorophenols (cps) and their derivatives are persistent environmental pollutants which are used in the manufacture of dyes, drugs, pesticides and other industrial products. cps, which include monochlorophenols, polychlorophenols, chloronitrophenols, chloroaminophenols and chloromethylphenols, are highly toxic to living beings due to their carcinogenic, mutagenic and cytotoxic properties. several physico-chemical and biological methods have been used for removal of cps from the environment. bact ... | 2014 | 24589366 |
| antibacterial activity of rhizome of curcuma aromatica and partial purification of active compounds. | the hexane extract of curcuma aromatica, a plant belonging to the family zingiberaceae was tested on 10 bacterial strains (clinical isolates and standard strains). agar diffusion method was adopted for determining the antibacterial activity of the extract. the hexane extract was found to be active against all gram-positive strains tested, but inactive against gram-negative strains. the minimum inhibitory concentration and minimum bactericidal concentration were determined and found to be 539 μg/ ... | 2013 | 24591751 |
| characterization of 3-phosphoglycerate kinase from corynebacterium glutamicum and its impact on amino acid production. | corynebacterium glutamicum cg1790/pgk encodes an enzyme active as a 3-phosphoglycerate kinase (pgk) (ec 2.7.2.3) catalyzing phosphoryl transfer from 1,3-biphosphoglycerate (bpg) to adp to yield 3-phosphoglycerate (3-pg) and atp in substrate chain phosphorylation. | 2014 | 24593686 |
| classification of healthcare-associated infection: a systematic review 10 years after the first proposal. | ten years after the first proposal, a consensus definition of healthcare-associated infection (hcai) has not been reached, preventing the development of specific treatment recommendations. a systematic review of all definitions of hcai used in clinical studies is made. | 2014 | 24597462 |
| engineering non-heme mono- and dioxygenases for biocatalysis. | oxygenases are ubiquitous enzymes that catalyze the introduction of one or two oxygen atoms to unreactive chemical compounds. they require reduction equivalents from nadh or nadph and comprise metal ions, metal ion complexes, or coenzymes in their active site. thus, for industrial purposes, oxygenases are most commonly employed using whole cell catalysis, to alleviate the need for co-factor regeneration. biotechnological applications include bioremediation, chiral synthesis, biosensors, fine che ... | 2012 | 24688652 |
| microbial growth and carbon use efficiency in the rhizosphere and root-free soil. | plant-microbial interactions alter c and n balance in the rhizosphere and affect the microbial carbon use efficiency (cue)-the fundamental characteristic of microbial metabolism. estimation of cue in microbial hotspots with high dynamics of activity and changes of microbial physiological state from dormancy to activity is a challenge in soil microbiology. we analyzed respiratory activity, microbial dna content and cue by manipulation the c and nutrients availability in the soil under beta vulgar ... | 2014 | 24722409 |
| isolation and characterization of broad spectrum coaggregating bacteria from different water systems for potential use in bioaugmentation. | the bridging bacteria with broad-spectrum coaggregation ability play an important role during multispecies-biofilm development. in this study, through a visual and semi-quantitative assay, twenty-two bacterial strains with aggregation ability were obtained from 8 different water environments, and these strains were assigned to 7 genera according to their 16s rdna and they were aeromonas, bacillus, comamonas, exiguobacterium, pseudomonas, shewanella and comamonas. furthermore, all possible 231 pa ... | 2014 | 24736645 |
| food, pathogen, signal: the multifaceted nature of a bacterial diet. | c. elegans, both in the wild and in the lab, live on a diet of live bacteria. the bacterial diet provides nutrients for c. elegans, but can also play a number of other roles in c. elegans physiology. recently, we compared the effects of different bacterial diets on life history traits and gene expression. here, we discuss our recent findings in the context of other dietary studies and highlight challenges in understanding dietary effects. for instance, since bacteria can be pathogenic it can be ... | 2013 | 24744980 |
| fast and economic immobilization methods described for non-commercial pseudomonas lipases. | there is an increasing interest to seek new enzyme preparations for the development of new products derived from bioprocesses to obtain alternative bio-based materials. in this context, four non-commercial lipases from pseudomonas species were prepared, immobilized on different low-cost supports, and examined for potential biotechnological applications. | 2014 | 24755191 |
| characterization and function of the first antibiotic isolated from a vent organism: the extremophile metazoan alvinella pompejana. | the emblematic hydrothermal worm alvinella pompejana is one of the most thermo tolerant animal known on earth. it relies on a symbiotic association offering a unique opportunity to discover biochemical adaptations that allow animals to thrive in such a hostile habitat. here, by studying the pompeii worm, we report on the discovery of the first antibiotic peptide from a deep-sea organism, namely alvinellacin. after purification and peptide sequencing, both the gene and the peptide tertiary struct ... | 2014 | 24776651 |
| the dimerization domain in dape enzymes is required for catalysis. | the emergence of antibiotic-resistant bacterial strains underscores the importance of identifying new drug targets and developing new antimicrobial compounds. lysine and meso-diaminopimelic acid are essential for protein production and bacterial peptidoglycan cell wall remodeling and are synthesized in bacteria by enzymes encoded within dap operon. therefore dap enzymes may serve as excellent targets for developing a new class of antimicrobial agents. the dape-encoded n-succinyl-l,l-diaminopimel ... | 2014 | 24806882 |
| inhibition of batrachochytrium dendrobatidis caused by bacteria isolated from the skin of boreal toads, anaxyrus (bufo) boreas boreas, from grand teton national park, wyoming, usa. | the chytrid fungus batrachochytrium dendrobatidis is a significant cause of the worldwide decline in amphibian populations; however, various amphibian species are capable of coexisting with b. dendrobatidis. among them are boreal toads (anaxyrus (bufo) boreas boreas) located in grand teton national park (gtnp) in wyoming, usa. the purpose of this study was to identify cultivable bacterial isolates from the skin microbiota of boreal toads from gtnp and determine if they were capable of inhibiting ... | 2014 | 24826077 |
| prevent infection linked to the dialysis water in a hemodialysis center in fez city (morocco). | water treatment systems are a critical variable in dialysis therapy. rigorous control of hemodialysis water quality is particularly important in order to guarantee a better quality of life of the hemodialysis patients. the objective of the study was to evaluate the chemical, microbiological quality and antimicrobial resistance of bacteria isolated from water and dialysate in a public hd center. | 2013 | 24839530 |
| u2 snrnp is required for expression of the 3' end of genes. | pre-mrna in eukaryotes is subjected to mrna processing, which includes capping, polyadenylation, and splicing. transcription and mrna processing are coupled, and this coupling stimulates mrna processing; however, the effects of mrna processing on transcription are not fully understood. in this study, we found that inhibition of u2 snrnp by a splicing inhibitor, spliceostatin a (ssa), or by an antisense oligonucleotide to u2 snrna, caused gene-specific 3'-end down-regulation. removal of ssa from ... | 2014 | 24845214 |
| evolving epidemiology and antimicrobial resistance in spontaneous bacterial peritonitis: a two-year observational study. | current recommendations for empirical antimicrobial therapy in spontaneous bacterial peritonitis (sbp) are based on quite old trials. since microbial epidemiology and the management of patients have changed, whether these recommendations are still appropriate must be confirmed. | 2014 | 24884471 |
| natural rice rhizospheric microbes suppress rice blast infections. | the natural interactions between plant roots and their rhizospheric microbiome are vital to plant fitness, modulating both growth promotion and disease suppression. in rice (oryza sativa), a globally important food crop, as much as 30% of yields are lost due to blast disease caused by fungal pathogen magnaporthe oryzae. capitalizing on the abilities of naturally occurring rice soil bacteria to reduce m. oryzae infections could provide a sustainable solution to reduce the amount of crops lost to ... | 2014 | 24884531 |
| the gut microbiota of larvae of rhynchophorus ferrugineus oliver (coleoptera: curculionidae). | the red palm weevil (rpw) rhynchophorus ferrugineus olivier (coleoptera: curculionidae) is one of the major pests of palms. the larvae bore into the palm trunk and feed on the palm tender tissues and sap, leading the host tree to death. the gut microbiota of insects plays a remarkable role in the host life and understanding the relationship dynamics between insects and their microbiota may improve the biological control of insect pests. the purpose of this study was to analyse the diversity of t ... | 2014 | 24884866 |
| biofertilizers function as key player in sustainable agriculture by improving soil fertility, plant tolerance and crop productivity. | current soil management strategies are mainly dependent on inorganic chemical-based fertilizers, which caused a serious threat to human health and environment. the exploitation of beneficial microbes as a biofertilizer has become paramount importance in agriculture sector for their potential role in food safety and sustainable crop production. the eco-friendly approaches inspire a wide range of application of plant growth promoting rhizobacteria (pgprs), endo- and ectomycorrhizal fungi, cyanobac ... | 2014 | 24885352 |
| a novel, nested, multiplex, real-time pcr for detection of bacteria and fungi in blood. | the study describes the application of the pcr method for the simultaneous detection of dna of gram-negative bacteria, gram-positive bacteria, yeast fungi and filamentous fungi in blood and, thus, a whole range of microbial etiological agents that may cause sepsis. material for the study was sterile blood inoculated with four species of microorganisms (escherichia coli, staphylococcus aureus, candida albicans and aspergillus fumigatus) and blood collected from patients with clinical symptoms of ... | 2014 | 24893651 |
| new insights into 1-aminocyclopropane-1-carboxylate (acc) deaminase phylogeny, evolution and ecological significance. | the main objective of this work is the study of the phylogeny, evolution and ecological importance of the enzyme 1-aminocyclopropane-1-carboxylate (acc) deaminase, the activity of which represents one of the most important and studied mechanisms used by plant growth-promoting microorganisms. the acc deaminase gene and its regulatory elements presence in completely sequenced organisms was verified by multiple searches in diverse databases, and based on the data obtained a comprehensive analysis w ... | 2014 | 24905353 |
| quorum quenching mediated approaches for control of membrane biofouling. | membrane biofouling is widely acknowledged as the most frequent adverse event in wastewater treatment systems resulting in significant loss of treatment efficiency and economy. different strategies including physical cleaning and use of antimicrobial chemicals or antibiotics have been tried for reducing membrane biofouling. such traditional practices are aimed to eradicate biofilms or kill the bacteria involved, but the greater efficacy in membrane performance would be achieved by inhibiting bio ... | 2014 | 24910534 |
| cyanuric acid hydrolase from azorhizobium caulinodans ors 571: crystal structure and insights into a new class of ser-lys dyad proteins. | cyanuric acid hydrolase (cah) catalyzes the hydrolytic ring-opening of cyanuric acid (2,4,6-trihydroxy-1,3,5-triazine), an intermediate in s-triazine bacterial degradation and a by-product from disinfection with trichloroisocyanuric acid. in the present study, an x-ray crystal structure of the cah-barbituric acid inhibitor complex from azorhizobium caulinodans ors 571 has been determined at 2.7 å resolution. the cah protein fold consists of three structurally homologous domains forming a β-barre ... | 2014 | 24915109 |
| teleost microbiomes: the state of the art in their characterization, manipulation and importance in aquaculture and fisheries. | indigenous microbiota play a critical role in the lives of their vertebrate hosts. in human and mouse models it is increasingly clear that innate and adaptive immunity develop in close concert with the commensal microbiome. furthermore, several aspects of digestion and nutrient metabolism are governed by intestinal microbiota. research on teleosts has responded relatively slowly to the introduction of massively parallel sequencing procedures in microbiomics. nonetheless, progress has been made i ... | 2014 | 24917852 |
| from community approaches to single-cell genomics: the discovery of ubiquitous hyperhalophilic bacteroidetes generalists. | the microbiota of multi-pond solar salterns around the world has been analyzed using a variety of culture-dependent and molecular techniques. however, studies addressing the dynamic nature of these systems are very scarce. here we have characterized the temporal variation during 1 year of the microbiota of five ponds with increasing salinity (from 18% to >40%), by means of card-fish and dgge. microbial community structure was statistically correlated with several environmental parameters, includ ... | 2014 | 24926861 |
| from community approaches to single-cell genomics: the discovery of ubiquitous hyperhalophilic bacteroidetes generalists. | the microbiota of multi-pond solar salterns around the world has been analyzed using a variety of culture-dependent and molecular techniques. however, studies addressing the dynamic nature of these systems are very scarce. here we have characterized the temporal variation during 1 year of the microbiota of five ponds with increasing salinity (from 18% to >40%), by means of card-fish and dgge. microbial community structure was statistically correlated with several environmental parameters, includ ... | 2014 | 24926861 |
| melioidosis presenting as lymphadenitis: a case report. | melioidosis is an infection caused by the facultative intracellular gram-negative bacterium; burkholderia pseudomallei. it gives rise to protean clinical manifestations and has a varied prognosis. although it was rare in sri lanka increasing numbers of cases are being reported with high morbidity and mortality. here we report a case of melioidosis presenting with lymphadenitis which was diagnosed early and treated promptly with a good outcome. | 2014 | 24927768 |
| microbial community structures and dynamics in the o3/bac drinking water treatment process. | effectiveness of drinking water treatment, in particular pathogen control during the water treatment process, is always a major public health concern. in this investigation, the application of pcr-dgge technology to the analysis of microbial community structures and dynamics in the drinking water treatment process revealed several dominant microbial populations including: α-proteobacteria, β-proteobacteria, γ-proteobacteria, bacteroidetes, actinobacteria firmicutes and cyanobacteria. α-proteobac ... | 2014 | 24937529 |
| a novel peptidoglycan binding protein crucial for pbp1a-mediated cell wall biogenesis in vibrio cholerae. | the bacterial cell wall, which is comprised of a mesh of polysaccharide strands crosslinked via peptide bridges (peptidoglycan, pg), is critical for maintenance of cell shape and survival. pg assembly is mediated by a variety of penicillin binding proteins (pbp) whose fundamental activities have been characterized in great detail; however, there is limited knowledge of the factors that modulate their activities in different environments or growth phases. in vibrio cholerae, the cause of cholera, ... | 2014 | 24945690 |
| computational design of a ph stable enzyme: understanding molecular mechanism of penicillin acylase's adaptation to alkaline conditions. | protein stability provides advantageous development of novel properties and can be crucial in affording tolerance to mutations that introduce functionally preferential phenotypes. consequently, understanding the determining factors for protein stability is important for the study of structure-function relationship and design of novel protein functions. thermal stability has been extensively studied in connection with practical application of biocatalysts. however, little work has been done to ex ... | 2014 | 24959852 |
| comparison of bacterial community structure and dynamics during the thermophilic composting of different types of solid wastes: anaerobic digestion residue, pig manure and chicken manure. | this study investigated the impact of composting substrate types on the bacterial community structure and dynamics during composting processes. to this end, pig manure (pm), chicken manure (cm), a mixture of pm and cm (pm + cm), and a mixture of pm, cm and anaerobic digestion residue (adr) (pm + cm + adr) were selected for thermophilic composting. the bacterial community structure and dynamics during the composting process were detected and analysed by polymerase chain reaction-denaturing gradie ... | 2014 | 24963997 |
| identification and characterization of the spiruchostatin biosynthetic gene cluster enable yield improvement by overexpressing a transcriptional activator. | spiruchostatins a and b are members of the fk228-family of natural products with potent histone deacetylase inhibitory activities and antineoplastic activities. however, their production in the wild-type strain of pseudomonas sp. q71576 is low. to improve the yield, the spiruchostatin biosynthetic gene cluster (spi) was first identified by rapid genome sequencing and characterized by genetic mutations. this spi gene cluster encodes a hybrid biosynthetic pathway similar to that encoded by the fk2 ... | 2014 | 24973954 |
| rate of positive urine culture and double-j catheters colonization on the basis of microorganism dna analysis. | the aim of the trial was to estimate the relationship between colonization of the double-j catheter, and the microorganisms cultured from urine. | 2014 | 24982789 |
| sepsis in intensive care unit patients with traumatic brain injury: factors associated with higher mortality. | patients with traumatic brain injury are particularly susceptible to sepsis, which may exacerbate the systemic inflammatory response and lead to organ dysfunction. the influence of clinical variables on the mortality of intensive care unit patients with traumatic brain injury and sepsis was investigated. | 2014 | 25028949 |
| spatial heterogeneity of bacteria: evidence from hot composts by culture-independent analysis. | the phylogenetic diversity of the bacteria in hot composting samples collected from three spatial locations was investigated by molecular tools in order to determine the influence of gradient effect on bacterial communities during the thermophilic phase of composting swine manure with rice straw. total microbial dna was extracted and bacterial near full-length 16s rrna genes were subsequently amplified, cloned, restriction fragment length polymorphism-screened and sequenced. the superstratum sam ... | 2012 | 25049662 |
| two structurally different dienelactone hydrolases (tfdei and tfdeii) from cupriavidus necator jmp134 plasmid pjp4 catalyse cis- and trans-dienelactones with similar efficiency. | in this study, dienelactone hydrolases (tfdei and tfdeii) located on plasmid pjp4 of cupriavidus necator jmp134 were cloned, purified, characterized and three dimensional structures were predicted. tfdei and tfdeii genes were cloned into pet21b vector and expressed in e. coli bl21(de3). the enzymes were purified by applying ultra-membrane filtration, anion-exchange qff and gel-filtration columns. the enzyme activity was determined by using cis-dienelactone. the three-dimensional structure of enz ... | 2014 | 25054964 |
| efficient asymmetric synthesis of 1-cyano-tetrahydroisoquinolines from lipase dual activity and opposite enantioselectivities in α-aminonitrile resolution. | dual promiscuous racemization/amidation activities of lipases leading to efficient dynamic kinetic resolution protocols of racemic α-aminonitrile compounds are described. α-amidonitrile products of high enantiomeric purity could be formed in high yields. several lipases from different sources were shown to exhibit the dual catalytic activities, where opposite enantioselectivities could be recorded for certain substrates. | 2014 | 25055970 |
| structural and functional analysis show that the escherichia coli uncharacterized protein yjcs is likely an alkylsulfatase. | sodium dodecyl sulfate (sds) is a widely used anionic surfactant in industry and research settings, and is known to have a detrimental effect to the environment. the pathway of sds degradation by bacteria is initiated by an alkylsulfatase and the oxidized product, 1-dodecanoic acid, subsequently enters into the β-oxidation pathway and is used as a carbon source. in this work, we solved the crystal structure of escherichia coli uncharacterized protein yjcs and identified that it belongs to the ty ... | 2014 | 25066955 |
| newly identified pathogens associated with periodontitis: a systematic review. | there is substantial evidence supporting the role of certain oral bacteria species in the onset and progression of periodontitis. nevertheless, results of independent-culture diagnostic methods introduced about a decade ago have pointed to the existence of new periodontal pathogens. however, the data of these studies have not been evaluated together, which may generate some misunderstanding on the actual role of these microorganisms in the etiology of periodontitis. the aim of this systematic re ... | 2014 | 25074492 |
| identification of a novel nidovirus in an outbreak of fatal respiratory disease in ball pythons (python regius). | respiratory infections are important causes of morbidity and mortality in reptiles; however, the causative agents are only infrequently identified. | 2014 | 25106433 |
| antibiotic susceptibilities of bacteria isolated within the oral flora of florida blacktip sharks: guidance for empiric antibiotic therapy. | sharks possess a variety of pathogenic bacteria in their oral cavity that may potentially be transferred into humans during a bite. the aim of the presented study focused on the identification of the bacteria present in the mouths of live blacktip sharks, carcharhinus limbatus, and the extent that these bacteria possess multi-drug resistance. swabs were taken from the oral cavity of nineteen live blacktip sharks, which were subsequently released. the average fork length was 146 cm (±11), suggest ... | 2014 | 25110948 |
| degradation of toluene by ortho cleavage enzymes in burkholderia fungorum flu100. | burkholderia fungorum flu100 simultaneously oxidized any mixture of toluene, benzene and mono-halogen benzenes to (3-substituted) catechols with a selectivity of nearly 100%. further metabolism occurred via enzymes of ortho cleavage pathways with complete mineralization. during the transformation of 3-methylcatechol, 4-carboxymethyl-2-methylbut-2-en-4-olide (2-methyl-2-enelactone, 2-ml) accumulated transiently, being further mineralized only after a lag phase of 2 h in case of cells pre-grown on ... | 2014 | 25130674 |
| degradation of toluene by ortho cleavage enzymes in burkholderia fungorum flu100. | burkholderia fungorum flu100 simultaneously oxidized any mixture of toluene, benzene and mono-halogen benzenes to (3-substituted) catechols with a selectivity of nearly 100%. further metabolism occurred via enzymes of ortho cleavage pathways with complete mineralization. during the transformation of 3-methylcatechol, 4-carboxymethyl-2-methylbut-2-en-4-olide (2-methyl-2-enelactone, 2-ml) accumulated transiently, being further mineralized only after a lag phase of 2 h in case of cells pre-grown on ... | 2014 | 25130674 |
| does netosis contribute to the bacterial pathoadaptation in cystic fibrosis? | significant advances in our understanding of neutrophil biology were made in the past several years. the exciting discovery that neutrophils deploy neutrophil extracellular traps (nets) to catch pathogens paved the way for a series of additional studies to define the molecular mechanisms of net generation and the biological significance of netosis in acute and chronic pathologic conditions. this review highlights the latest knowledge regarding net structures, deployment, and function, with an em ... | 2014 | 25157250 |
| quality parameters, fatty acid profiling and estimation of umbelliferone in grahaṇimihira tailam: an ayurvedic oil preparation. | grahaṇimihira tailam is an unexplored ayurvedic oil preparation which consists of 34 ingredients. the efficacy of this traditional ayurvedic medicine is undisputable. proper clinical standardization of this formulation will go a long way in securing greater recognition for it. the main objective of this study was to develop standardization parameters for the formulation in a multidisciplinary way. | 2013 | 25161324 |
| characterization of the medium- and long-chain n-alkanes degrading pseudomonas aeruginosa strain sjtd-1 and its alkane hydroxylase genes. | a gram-negative aliphatic hydrocarbon-degrading bacterium sjtd-1 isolated from oil-contaminated soil was identified as pseudomonas aeruginosa by comparative analyses of the 16s rrna sequence, phenotype, and physiological features. sjtd-1 could efficiently mineralize medium- and long-chain n-alkanes (c12-c30) as its sole carbon source within seven days, showing the most optimal growth on n-hexadecane, followed by n-octadecane, and n-eicosane. in 36 h, 500 mg/l of tetradecane, hexadecane, and octa ... | 2014 | 25165808 |
| p. aeruginosa sgnh hydrolase-like proteins algj and algx have similar topology but separate and distinct roles in alginate acetylation. | the o-acetylation of polysaccharides is a common modification used by pathogenic organisms to protect against external forces. pseudomonas aeruginosa secretes the anionic, o-acetylated exopolysaccharide alginate during chronic infection in the lungs of cystic fibrosis patients to form the major constituent of a protective biofilm matrix. four proteins have been implicated in the o-acetylation of alginate, algijf and algx. to probe the biological function of algj, we determined its structure to 1 ... | 2014 | 25165982 |
| synonymous codon bias and functional constraint on gc3-related dna backbone dynamics in the prokaryotic nucleoid. | while mrna stability has been demonstrated to control rates of translation, generating both global and local synonymous codon biases in many unicellular organisms, this explanation cannot adequately explain why codon bias strongly tracks neighboring intergene gc content; suggesting that structural dynamics of dna might also influence codon choice. because minor groove width is highly governed by 3-base periodicity in gc, the existence of triplet-based codons might imply a functional role for the ... | 2014 | 25200075 |
| outcomes of patients with healthcare-associated pneumonia: worse disease or sicker patients? | healthcare-associated pneumonia (hcap) is an entity distinct from community-acquired pneumonia (cap). hcap has a higher case-fatality rate, due either to hcap organisms or to the health status of hcap patients. the contribution of hcap criteria to case-fatality rate is unknown. | 2014 | 25222889 |
| rhamnolipid biosurfactants-past, present, and future scenario of global market. | 2014 | 25228898 | |
| isolation and characterization of atrazine mineralizing bacillus subtilis strain hb-6. | atrazine is a widely used herbicide with great environmental concern due to its high potential to contaminate soil and waters. an atrazine-degrading bacterial strain hb-6 was isolated from industrial wastewater and the 16s rrna gene sequencing identified hb-6 as a bacillus subtilis. pcr assays indicated that hb-6 contained atrazine-degrading genes trzn, atzb and atzc. the strain hb-6 was capable of utilizing atrazine and cyanuric acid as a sole nitrogen source for growth and even cleaved the s-t ... | 2014 | 25238246 |
| growth kinetics, effect of carbon substrate in biosynthesis of mcl-pha by pseudomonas putida bet001. | growth associated biosynthesis of medium chain length poly-3-hydroxyalkanoates (mcl-pha) in pseudomonas putida bet001 isolated from palm oil mill effluent was studied. models with substrate inhibition terms described well the kinetics of its growth. selected fatty acids (c8:0 to c18:1) and ammonium were used as carbon and nitrogen sources during growth and pha biosynthesis, resulting in pha accumulation of about 50 to 69% (w/w) and pha yields ranging from 10.12 g l(-1) to 15.45 g l(-1), respecti ... | 2014 | 25242925 |
| the non-canonical hydroxylase structure of yfcm reveals a metal ion-coordination motif required for ef-p hydroxylation. | ef-p is a bacterial trna-mimic protein, which accelerates the ribosome-catalyzed polymerization of poly-prolines. in escherichia coli, ef-p is post-translationally modified on a conserved lysine residue. the post-translational modification is performed in a two-step reaction involving the addition of a β-lysine moiety and the subsequent hydroxylation, catalyzed by poxa and yfcm, respectively. the β-lysine moiety was previously shown to enhance the rate of poly-proline synthesis, but the role of ... | 2014 | 25274739 |
| membrane bioprocesses for pharmaceutical micropollutant removal from waters. | the purpose of this review work is to give an overview of the research reported on bioprocesses for the treatment of domestic or industrial wastewaters (ww) containing pharmaceuticals. conventional ww treatment technologies are not efficient enough to completely remove all pharmaceuticals from water. indeed, these compounds are becoming an actual public health problem, because they are more and more present in underground and even in potable waters. different types of bioprocesses are described ... | 2014 | 25295629 |
| prophylactic antibody treatment and intramuscular immunization reduce infectious human rhinovirus 16 load in the lower respiratory tract of challenged cotton rats. | human rhinoviruses (hrv) represent the single most important etiological agents of the common cold and are the most frequent cause of acute respiratory infections in humans. currently the performance of available animal models for immunization studies using hrv challenge is very limited. the cotton rat (sigmodon hispidus) is a well-recognized model for the study of human respiratory viral infections. in this work we show that, without requiring any genetic modification of either the host or the ... | 2014 | 25328560 |
| bacterial communities in semen from men of infertile couples: metagenomic sequencing reveals relationships of seminal microbiota to semen quality. | some previous studies have identified bacteria in semen as being a potential factor in male infertility. however, only few types of bacteria were taken into consideration while using pcr-based or culturing methods. here we present an analysis approach using next-generation sequencing technology and bioinformatics analysis to investigate the associations between bacterial communities and semen quality. ninety-six semen samples collected were examined for bacterial communities, measuring seven cli ... | 2014 | 25340531 |
| chromobacterium csp_p reduces malaria and dengue infection in vector mosquitoes and has entomopathogenic and in vitro anti-pathogen activities. | plasmodium and dengue virus, the causative agents of the two most devastating vector-borne diseases, malaria and dengue, are transmitted by the two most important mosquito vectors, anopheles gambiae and aedes aegypti, respectively. insect-bacteria associations have been shown to influence vector competence for human pathogens through multi-faceted actions that include the elicitation of the insect immune system, pathogen sequestration by microbes, and bacteria-produced anti-pathogenic factors. t ... | 2014 | 25340821 |
| study of the structure-function-stability relationships in yeast d-amino acid oxidase: hydrophobization of alpha-helices. | hydrophobization of alpha-helices is one of the general approaches used for improving the thermal stability of enzymes. a total of 11 serine residues located in alpha-helices have been found based on multiple alignments of the amino acid sequences of d-amino acid oxidases from different organisms and the analysis of the 3d-structure of d-amino acid oxidase from yeast trigonopsis variabilis (tvdaao, ec 1.4.3.3). as a result of further structural analysis, eight ser residues in 67, 77, 78, 105, 27 ... | 2014 | 25349716 |
| draft genome sequence analysis of a pseudomonas putida w15oct28 strain with antagonistic activity to gram-positive and pseudomonas sp. pathogens. | pseudomonas putida is a member of the fluorescent pseudomonads known to produce the yellow-green fluorescent pyoverdine siderophore. p. putida w15oct28, isolated from a stream in brussels, was found to produce compound(s) with antimicrobial activity against the opportunistic pathogens staphylococcus aureus, pseudomonas aeruginosa, and the plant pathogen pseudomonas syringae, an unusual characteristic for p. putida. the active compound production only occurred in media with low iron content and w ... | 2014 | 25369289 |
| silencing by h-ns potentiated the evolution of salmonella. | the bacterial h-ns protein silences expression from sequences with higher at-content than the host genome and is believed to buffer the fitness consequences associated with foreign gene acquisition. loss of h-ns results in severe growth defects in salmonella, but the underlying reasons were unclear. an experimental evolution approach was employed to determine which secondary mutations could compensate for the loss of h-ns in salmonella. six independently derived s. typhimurium hns mutant strains ... | 2014 | 25375226 |
| reactive oxygen species mediated bacterial biofilm inhibition via zinc oxide nanoparticles and their statistical determination. | the formation of bacterial biofilm is a major challenge in clinical applications. the main aim of this study is to describe the synthesis, characterization and biocidal potential of zinc oxide nanoparticles (nps) against bacterial strain pseudomonas aeruginosa. these nanoparticles were synthesized via soft chemical solution process in a very short time and their structural properties have been investigated in detail by using x-ray diffraction and transmission electron microscopy measurements. in ... | 2014 | 25402188 |
| genomics-based exploration of virulence determinants and host-specific adaptations of pseudomonas syringae strains isolated from grasses. | the pseudomonas syringae species complex has recently been named the number one plant pathogen, due to its economic and environmental impacts, as well as for its role in scientific research. the bacterium has been repeatedly reported to cause outbreaks on bean, cucumber, stone fruit, kiwi and olive tree, as well as on other crop and non-crop plants. it also serves as a model organism for research on the type iii secretion system (t3ss) and plant-pathogen interactions. while most of the current w ... | 2014 | 25437611 |
| dissolving mechanism of strain p17 on insoluble phosphorus of yellow-brown soil. | strain p17 was a bacterial strain identified as bacillus megaterium isolated from ground accumulating phosphate rock powder. the fermentation broth of strain p17 and the yellow-brown soil from nanjing agricultural university garden were collected to conduct this study. the simulation of fixed insoluble phosphorous forms after applying calcium superphosphate into yellow-brown soil was performed in pots, while available p and total p of soil were extremely positive correlative with those of ground ... | 2014 | 25477929 |
| significance of serum antibodies against hsp 60 and hsp 70 for the diagnostic of infectious diseases. | heat shock proteins (hsp) represent important antigenic targets for the immune response, playing an important role in the pathology and infectious diseases control. the purpose of this work was to investigate the levels of hsp60 and hsp70 specific antibodies in the bloodstream of patients with different bacterial infections and cancer, in order to evaluate their potential role as diagnosis markers of different infectious diseases. detection of specific anti-hsp 60 and hsp 70 serum levels was per ... | 2014 | 25483863 |
| bioconversion of α-pinene by a novel cold-adapted fungus chrysosporium pannorum. | the psychrotrophic fungus chrysosporium pannorum a-1 is reported for the first time as a novel biocatalyst for o2-promoted oxidation of α-pinene. gc-ms analysis indicated that the main products of the reaction were compounds of a high commercial value, verbenol (1) and verbenone (2). exponentially growing cells (days 2-3) were about twice as active as cells in the late stationary phase in terms of the total concentration of products. the highest yields of 1 and 2 were obtained using three-day an ... | 2014 | 25487757 |
| bioconversion of α-pinene by a novel cold-adapted fungus chrysosporium pannorum. | the psychrotrophic fungus chrysosporium pannorum a-1 is reported for the first time as a novel biocatalyst for o2-promoted oxidation of α-pinene. gc-ms analysis indicated that the main products of the reaction were compounds of a high commercial value, verbenol (1) and verbenone (2). exponentially growing cells (days 2-3) were about twice as active as cells in the late stationary phase in terms of the total concentration of products. the highest yields of 1 and 2 were obtained using three-day an ... | 2014 | 25487757 |
| bacteria-bacteria interactions within the microbiota of the ancestral metazoan hydra contribute to fungal resistance. | epithelial surfaces of most animals are colonized by diverse microbial communities. although it is generally agreed that commensal bacteria can serve beneficial functions, the processes involved are poorly understood. here we report that in the basal metazoan hydra, ectodermal epithelial cells are covered with a multilayered glycocalyx that provides a habitat for a distinctive microbial community. removing this epithelial microbiota results in lethal infection by the filamentous fungus fusarium ... | 2014 | 25514534 |
| bacteria-bacteria interactions within the microbiota of the ancestral metazoan hydra contribute to fungal resistance. | epithelial surfaces of most animals are colonized by diverse microbial communities. although it is generally agreed that commensal bacteria can serve beneficial functions, the processes involved are poorly understood. here we report that in the basal metazoan hydra, ectodermal epithelial cells are covered with a multilayered glycocalyx that provides a habitat for a distinctive microbial community. removing this epithelial microbiota results in lethal infection by the filamentous fungus fusarium ... | 2014 | 25514534 |
| novel assay to measure the plasmid mobilizing potential of mixed microbial communities. | mobilizable plasmids lack necessary genes for complete conjugation and are therefore non-self-transmissible. instead, they rely on the conjugation system of conjugal plasmids to be horizontally transferred to new recipients. while community permissiveness, the fraction of a mixed microbial community that can receive self-transmissible conjugal plasmids, has been studied, the intrinsic ability of a community to mobilize plasmids that lack conjugation systems is unexplored. here, we present a nove ... | 2014 | 25566238 |
| applying nightingale charts to evaluate the heterogeneity of biomedical waste in a hospital. | to evaluate the heterogeneity of biomedical waste (bw) using nightingale charts. | 2014 | 25591088 |
| trapping and assembly of living colloids at water-water interfaces. | we study the assembly of inert and living colloids in a two-phase water-water system that provides an environment that can sustain bacteria, providing a new structure with rich potential to confine and structure microbial communities. the water-water system, formed via phase separation of a casein and xanthan mixture, forms a 3-d structure of coexisting casein-rich and xanthan-rich phases. fluorescent labelling and confocal microscopy reveal the attachment of these living colloids, including esc ... | 2015 | 25600991 |
| neonatal conjunctivitis - a review. | ophthalmia neonatorum remains a significant cause of ocular morbidity, blindness and even death in underdeveloped countries. the organisms causing ophthalmia neonatorum are acquired mainly from the mother's birth canal during delivery and a small percentage of cases are acquired by other ways. chlamydia and neisseria are the most common pathogens responsible for the perinatal infection. fortunately in most cases, laboratory studies can identify the causative organism and unlike other form of con ... | 2008 | 25606121 |
| nanomedicine and therapy of lung diseases. | the use of nanotechnology has significantly increased in different fields of science, including the development of drug delivery systems. currently, the most modern pharmaceutical nanocarriers, such as liposomes, micelles, nanoemulsions and polymeric nanoparticles, demonstrate extremely useful properties from the point of view of drug therapy. in this context, the development of nanocarriers for pulmonary application has been much debated by the scientific community in recent decades. although r ... | 2014 | 25628213 |
| comparison of microbiomes from different niches of upper and lower airways in children and adolescents with cystic fibrosis. | changes in the airway microbiome may be important in the pathophysiology of chronic lung disease in patients with cystic fibrosis. however, little is known about the microbiome in early cystic fibrosis lung disease and the relationship between the microbiomes from different niches in the upper and lower airways. therefore, in this cross-sectional study, we examined the relationship between the microbiome in the upper (nose and throat) and lower (sputum) airways from children with cystic fibrosis ... | 2015 | 25629612 |
| marine sediments microbes capable of electrode oxidation as a surrogate for lithotrophic insoluble substrate metabolism. | little is known about the importance and/or mechanisms of biological mineral oxidation in sediments, partially due to the difficulties associated with culturing mineral-oxidizing microbes. we demonstrate that electrochemical enrichment is a feasible approach for isolation of microbes capable of gaining electrons from insoluble minerals. to this end we constructed sediment microcosms and incubated electrodes at various controlled redox potentials. negative current production was observed in incub ... | 2014 | 25642220 |
| marine sediments microbes capable of electrode oxidation as a surrogate for lithotrophic insoluble substrate metabolism. | little is known about the importance and/or mechanisms of biological mineral oxidation in sediments, partially due to the difficulties associated with culturing mineral-oxidizing microbes. we demonstrate that electrochemical enrichment is a feasible approach for isolation of microbes capable of gaining electrons from insoluble minerals. to this end we constructed sediment microcosms and incubated electrodes at various controlled redox potentials. negative current production was observed in incub ... | 2014 | 25642220 |
| identification of a histidine metal ligand in the arge-encoded n-acetyl-l-ornithine deacetylase from escherichia coli. | the h355a, h355k, h80a, and h80k mutant enzymes of the arge-encoded n-acetyl-l-ornithine deacetylase (arge) from escherichia coli were prepared, however, only the h355a enzyme was found to be soluble. kinetic analysis of the co(ii)-loaded h355a exhibited activity levels that were 380-fold less than co(ii)-loaded wt arge. electronic absorption spectra of co(ii)-loaded h355a-arge indicate that the bound co(ii) ion resides in a distorted, five-coordinate environment and isothermal titration calorim ... | 2013 | 25674394 |
| statistically optimized biotransformation protocol for continuous production of l-dopa using mucuna monosperma callus culture. | l-dopa (3,4-dihydroxyphenyl-l-alanine), a modified amino acid, is an expansively used drug for the parkinson's disease treatment. in the present study, optimization of nutritional parameters influencing l-dopa production was attempted using the response surface methodology (rsm) from mucuna monosperma callus. optimization of the four factors was carried out using the box-behnken design. the optimized levels of factors predicted by the model include tyrosine 0.894 g l(-1), ph 4.99, ascorbic acid ... | 2013 | 25674405 |
| phosphate solubilizing microbes: sustainable approach for managing phosphorus deficiency in agricultural soils. | phosphorus is the second important key element after nitrogen as a mineral nutrient in terms of quantitative plant requirement. although abundant in soils, in both organic and inorganic forms, its availability is restricted as it occurs mostly in insoluble forms. the p content in average soil is about 0.05% (w/w) but only 0.1% of the total p is available to plant because of poor solubility and its fixation in soil (illmer and schinner, soil biol biochem 27:257-263, 1995). an adequate supply of p ... | 2013 | 25674415 |
| the induction of ethylene response factor 3 (erf3) in potato as a result of co-inoculation with pseudomonas sp. r41805 and rhizophagus irregularis mucl 41833 - a possible role in plant defense. | colonization of plant rhizosphere/roots by beneficial microorganisms (e.g. plant growth promoting rhizobacteria - pgpr, arbuscular mycorrhizal fungi - amf) confers broad-spectrum resistance to virulent pathogens and is known as induced systemic resistance (isr) and mycorrhizal-induced resistance (mir). isr or mir, an indirect mechanism for biocontrol, involves complex signaling networks that are regulated by several plant hormones, the most important of which are salicylic acid (sa), jasmonic ac ... | 2015 | 25723847 |
| soil salinity: a serious environmental issue and plant growth promoting bacteria as one of the tools for its alleviation. | salinity is one of the most brutal environmental factors limiting the productivity of crop plants because most of the crop plants are sensitive to salinity caused by high concentrations of salts in the soil, and the area of land affected by it is increasing day by day. for all important crops, average yields are only a fraction - somewhere between 20% and 50% of record yields; these losses are mostly due to drought and high soil salinity, environmental conditions which will worsen in many region ... | 2014 | 25737642 |
| soil salinity: a serious environmental issue and plant growth promoting bacteria as one of the tools for its alleviation. | salinity is one of the most brutal environmental factors limiting the productivity of crop plants because most of the crop plants are sensitive to salinity caused by high concentrations of salts in the soil, and the area of land affected by it is increasing day by day. for all important crops, average yields are only a fraction - somewhere between 20% and 50% of record yields; these losses are mostly due to drought and high soil salinity, environmental conditions which will worsen in many region ... | 2014 | 25737642 |
| a bioinformatic survey of distribution, conservation, and probable functions of luxr solo regulators in bacteria. | luxr solo transcriptional regulators contain both an autoinducer binding domain (abd; n-terminal) and a dna binding helix-turn-helix domain (hth; c-terminal), but are not associated with a cognate n-acyl homoserine lactone (ahl) synthase coding gene in the same genome. although a few luxr solos have been characterized, their distributions as well as their role in bacterial signal perception and other processes are poorly understood. in this study we have carried out a systematic survey of distri ... | 2015 | 25759807 |
| cultivable endophytic bacteria from leaf bases of agave tequilana and their role as plant growth promoters. | agave tequilana weber var. 'azul' is grown for the production of tequila, inulin and syrup. diverse bacteria inhabit plant tissues and play a crucial role for plant health and growth. in this study culturable endophytic bacteria were extracted from leaf bases of 100 healthy agave tequilana plants. in plant tissue bacteria occurred at mean population densities of 3 million cfu/g of fresh plant tissue. three hundred endophytic strains were isolated and 16s rdna sequences grouped the bacteria into ... | 2014 | 25763038 |
| cultivable endophytic bacteria from leaf bases of agave tequilana and their role as plant growth promoters. | agave tequilana weber var. 'azul' is grown for the production of tequila, inulin and syrup. diverse bacteria inhabit plant tissues and play a crucial role for plant health and growth. in this study culturable endophytic bacteria were extracted from leaf bases of 100 healthy agave tequilana plants. in plant tissue bacteria occurred at mean population densities of 3 million cfu/g of fresh plant tissue. three hundred endophytic strains were isolated and 16s rdna sequences grouped the bacteria into ... | 2014 | 25763038 |
| detection of metallo-beta lactamases among carbapenem-resistant pseudomonas aeruginosa. | carbapenems are important drugs used for the treatment of pseudomonas aeruginosa infections, however metallo-β-lactamases (mbl) are able to efficiently hydrolyze these classes of drugs. immediate detection of the mbl-producing p. aeruginosa is necessary in order to accurately treat infections caused by this organism. | 2014 | 25774271 |
| clinical outcomes and microbiological characteristics of severe pneumonia in cancer patients: a prospective cohort study. | pneumonia is the most frequent type of infection in cancer patients and a frequent cause of icu admission. the primary aims of this study were to describe the clinical and microbiological characteristics and outcomes in critically ill cancer patients with severe pneumonia. | 2015 | 25803690 |
| phytogenic compounds as alternatives to in-feed antibiotics: potentials and challenges in application. | this article summarizes current experimental knowledge on the efficacy, possible mechanisms and feasibility in the application of phytogenic products as feed additives for food-producing animals. phytogenic compounds comprise a wide range of plant-derived natural bioactive compounds and essential oils are a major group. numerous studies have demonstrated that phytogenic compounds have a variety of functions, including antimicrobial/antiviral, antioxidative and anti-inflammation effects and impro ... | 2015 | 25806623 |
| comparative analysis of wolbachia genomes reveals streamlining and divergence of minimalist two-component systems. | two-component regulatory systems are commonly used by bacteria to coordinate intracellular responses with environmental cues. these systems are composed of functional protein pairs consisting of a sensor histidine kinase and cognate response regulator. in contrast to the well-studied caulobacter crescentus system, which carries dozens of these pairs, the streamlined bacterial endosymbiont wolbachia pipientis encodes only two pairs: ccka/ctra and plec/pled. here, we used bioinformatic tools to co ... | 2015 | 25809075 |
| alginate lyase: review of major sources and classification, properties, structure-function analysis and applications. | alginate lyases catalyze the degradation of alginate, a complex copolymer of α-l-guluronate and its c5 epimer β-d-mannuronate. the enzymes have been isolated from various kinds of organisms with different substrate specificities, including algae, marine mollusks, marine and terrestrial bacteria, and some viruses and fungi. with the progress of structural biology, many kinds of alginate lyases of different polysaccharide lyases families have been characterized by obtaining crystal structures, and ... | 2015 | 25831216 |
| experimental design approach to the optimization of pahs bioremediation from artificially contaminated soil: application of variables screening development. | the effectiveness of bioremediation systems for pah-contaminated soil may be constrained by physicochemical properties of contaminants and environmental factors. information on what is the most effective factor in bioremediation process is essential in the decision of what stimulations can be taken to assist the biodegradation efficacy. | 2015 | 25834738 |
| bacteria isolated from bats inhibit the growth of pseudogymnoascus destructans, the causative agent of white-nose syndrome. | emerging infectious diseases are a key threat to wildlife. several fungal skin pathogens have recently emerged and caused widespread mortality in several vertebrate groups, including amphibians, bats, rattlesnakes and humans. white-nose syndrome, caused by the fungal skin pathogen pseudogymnoascus destructans, threatens several hibernating bat species with extinction and there are few effective treatment strategies. the skin microbiome is increasingly understood to play a large role in determini ... | 2015 | 25853558 |
| encapsulating peritoneal sclerosis - a rare and serious complication of peritoneal dialysis: case series. | encapsulating peritoneal sclerosis is a pathological entity mainly associated with peritoneal dialysis (pd). the clinical syndrome is characterized by various degrees of intestinal obstruction due to thickening, sclerosis and calcification of peritoneum resulting in the encapsulation and cocooning of the bowel. it is a rare but potentially devastating complication associated with a considerable morbidity and mortality. | 2014 | 25870687 |
| comparing two types of rabbit atg prior to reduced intensity conditioning allogeneic hematopoietic sct for hematologic malignancies. | different rabbit polyclonal antilymphocyte globulins (atgs) are used in allogeneic hematopoietic stem cell transplantation (allohsct) to prevent graft-versus-host disease (gvhd). we compared 2 different atgs in allohsct after reduced intensity conditioning (ric) for hematological malignancies. we reviewed 30 allohsct for hematologic malignancies performed between 2007 and 2010 with fludarabine and i.v. busulfan as conditioning regimen. patients alternatingly received thymoglobulin or atg-f. medi ... | 2015 | 25874131 |
| the crystal structure of d-threonine aldolase from alcaligenes xylosoxidans provides insight into a metal ion assisted plp-dependent mechanism. | threonine aldolases catalyze the pyridoxal phosphate (plp) dependent cleavage of threonine into glycine and acetaldehyde and play a major role in the degradation of this amino acid. in nature, l- as well as d-specific enzymes have been identified, but the exact physiological function of d-threonine aldolases (dtas) is still largely unknown. both types of enantio-complementary enzymes have a considerable potential in biocatalysis for the stereospecific synthesis of various β-hydroxy amino acids, ... | 2015 | 25884707 |
| a redox regulatory system critical for mycobacterial survival in macrophages and biofilm development. | survival of m. tuberculosis in host macrophages requires the eukaryotic-type protein kinase g, pkng, but the underlying mechanism has remained unknown. here, we show that pkng is an integral component of a novel redox homeostatic system, rhocs, which includes the ribosomal protein l13 and renu, a nudix hydrolase encoded by a gene adjacent to pkng. studies in m. smegmatis showed that pkng expression is uniquely induced by nadh, which plays a key role in metabolism and redox homeostasis. in vitro, ... | 2015 | 25884716 |
| the toluene o-xylene monooxygenase enzymatic activity for the biosynthesis of aromatic antioxidants. | monocyclic phenols and catechols are important antioxidant compounds for the food and pharmaceutic industries; their production through biotransformation of low-added value starting compounds is of major biotechnological interest. the toluene o-xylene monooxygenase (tomo) from pseudomonas sp. ox1 is a bacterial multicomponent monooxygenase (bmm) that is able to hydroxylate a wide array of aromatic compounds and has already proven to be a versatile biochemical tool to produce mono- and dihydroxyl ... | 2015 | 25915063 |