Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| [a case of canine brucellosis due to brucella abortus]. | 1969 | 4985208 | |
| agglutinin response of rabbits to combined pasteurella tularensis-brucella abortus vaccination. | a schedule was developed for the simultaneous production of a pasteurella tularensis-brucella abortus antiserum in rabbits. three doses of 10(8) viable p. tularensis lvs organisms were given intravenously at weekly intervals. one day prior to the final dose of p. tularensis, the rabbits received 10(9) viable cells of b. abortus strain 19 intravenously. the use of live vaccines, administered in this sequence, resulted in high agglutinin titers within 3 weeks. the maximal agglutinin titer to eithe ... | 1970 | 4985429 |
| experimental study on the protective antigen derived from the vaccine strain brucella abortus ba-19. | 1969 | 4985512 | |
| [isolation of brucella abortus from the organs of mink]. | 1969 | 4985671 | |
| the proliferative and anamnestic antibody response of rabbit lymphoid cells in vitro. ii. requirement for adherent and nonadherent cells of the responses to particulate antigens in spleen cell cultures. | both primary and secondary responses to sheep erythrocytes and to brucella abortus antigen have been obtained in cultures of dispersed rabbit spleen cells. removal of adherent cells by repeated incubation of spleen cells on absorbent cotton diminished the ability of the spleen cell suspensions to give secondary as well as primary responses in vitro. when comparing cultures made in dishes and in tubes, the loss of responsiveness after incubation on cotton was much more evident in the dish culture ... | 1970 | 4986217 |
| trimethoprim-sulphamethoxazole in enteric fevers. | clinical improvement was rapid in all 13 patients with enteric fever and one with brucella abortus infection treated with trimethoprim-sulphamethoxazole. there were no side-effects of the treatment and it was found easy to administer to toxic and delirious patients. | 1970 | 4989044 |
| isolation of brucella abortus from vaginal mucus samples. | 1969 | 4989379 | |
| illness following accidental self-inoculation of brucella abortus strain 19 vaccine. | 1970 | 4991107 | |
| immuno-electrophoretic characterization of brucella abortus antigenic strain 99. ii. chemical extracts. | 1970 | 4991969 | |
| reversion of rough (r) to smooth (s) forms in brucella abortus. ii. comparison of the sii form obtained after reversion with the initial si form, as well as ri and rii rough variants. | 1970 | 4992358 | |
| differentiation of the serologicl response to yersinia enterocolitica and brucella abortus in cattle. | the serological responses of cattle to inoculation with brucella abortus and yersinia enterocolitica type ix were compared. complete cross-reactions were found in serum agglutination, antiglobulin, complement fixation and rose bengal plate tests. the cross-reaction between br. abortus and y. enterocolitica ix was confirmed by immunodiffusion tests. although antibodies specific for each organism could also be detected by immunodiffusion tests with high titre rabbit or bovine sera, these tests wer ... | 1970 | 4992575 |
| [homogeneity of different brucella abortus strains by means of lysotyping]. | 1967 | 4993371 | |
| persistence of brucella abortus, strain 19 infection in immunized cattle. | 1969 | 4993565 | |
| sero-chemical characterization of a soluble antigen extracted from brucella abortus. | 1970 | 4993699 | |
| the occurrence of agglutinating, complement-fixing, and incomplete antibodies to brucella abortus in milk, and their relationship to the milk ring test. | 1971 | 4993976 | |
| transfer of antibody production with cells from bursa of fabricius. | f(1) hybrid chicks isogenic for the strong b histocompatibility locus and for most weak h-loci were x-irradiated on day 1 after hatching, injected intraperitoneally on day 2 with dispersed cells of bursa, spleen, or thymus from 4- or 10-wk-old f(1) hybrid donors, and immediately challenged by the same route with either brucella abortus, sheep erythrocytes, or a mixture of both together. the agglutinin titers were measured in sera obtained 1 wk later. with 4-wk-old donors, a greater primary respo ... | 1970 | 4994444 |
| [immunostimulating effect of an imidotiiazole in the immunization of mice against brucella abortus infection]. | 1971 | 4995369 | |
| the use of various extracts of brucella abortus for haemagglutination test in brucellosis. | 1971 | 4998500 | |
| a purified killed brucella abortus vaccine. | 1971 | 4998841 | |
| immunisation of laboratory animals and cattle with non-agglutinogenic extracts of brucella abortus strain 45-20. | 1971 | 4998854 | |
| [studies on the complement fixation test in brucellosis. iv. correlation between titers by agglutination and by complement fixation determined with brucella abortus antigens in human and inoculated rabbits' sera]. | 1970 | 4999678 | |
| electron microscopy of tissue culture cells infected with brucella abortus. | thin sections of hamster kidney tissue cultures were examined by electron microscopy over a 7-day period after infection with brucella abortus 3183. numerous bacteria and structures resembling l-forms were present both intracellularly and extracellularly after the first 24 hr of infection. most intracellular microorganisms were enclosed by a cytoplasmic membrane, but in a few instances no limiting membrane was detected. after 4 to 7 days, fewer microorganisms were present, and most normal-appear ... | 1971 | 5001204 |
| the effect of erythritol on the growth of s19 and other attenuated strains of brucella abortus. | 1967 | 5001511 | |
| microagglutination procedures for febrile agglutination tests. | febrile agglutination tests were done by using as antigens brucella abortus, salmonella group d, proteus ox19, and pasteurella tularensis. comparison of results from 23 sera showed that the microtechnique, rapid slide, and test tube methods gave similar titers, although those from the microtechnique were generally higher. the sensitivity of the microtechnique depended upon the concentration of antigen, and, to obtain reproducible results, the optimal concentration of antigens had to be determine ... | 1971 | 5002142 |
| [experimental brucellosis in cattle. ii. distribution of brucella abortus 6 weeks after parturition and 3 to 5 and one-half months after experimental infection]. | 1970 | 5003298 | |
| [experimental bovine brucellosis. 3. the vaginal excretion of brucella abortus before and after parturition]. | 1970 | 5003299 | |
| antibody response, delayed hypersensitivity, and immunity in guinea pigs induced by smooth and rough strains of brucella abortus. | 1971 | 5004116 | |
| [lysogenic variations of brucella abortus. ii. variations obtained in vitro]. | 1971 | 5005071 | |
| antigenic relationship of brucella ovis to brucella abortus and brucella melitensis using the complement fixation test. | 1969 | 5005098 | |
| [vaccination of cows with live dessau brucella abortus vaccine]. | 1971 | 5104381 | |
| simultaneous immunization of cattle and with strain 19 brucella abortus and infectious bovine rhinotracheitis (ibr) vaccines. | 1965 | 5212981 | |
| the use of milk and blood tests to identify cows excreting brucella abortus in their milk in brucellosis problem herds. | 1965 | 5213210 | |
| stability of brucella abortus vaccine. | 1965 | 5216707 | |
| natural brucella infection in argentine wild foxes. | in the course of an investigation in 1962-64 into the natural occurrence of brucellosis among grey foxes in argentina, agglutination tests were performed on 728 sera of the foxes dusicyon gymnocercus antiquus and d. griseus griseus, captured in the provinces of buenos aires and rio negro. agglutination titres of from 1:25 to 1:800 were found in 173 (23.8%) of the foxes tested, 11.3% having titres of 1:100 or more. in bacteriological testing, eight cultures of brucella abortus, biotype 1, were ob ... | 1966 | 5296540 |
| the functions of the thymus system and the bursa system in the chicken. | the bursa of fabricius and the thymus are "central lymphoid organs" in the chicken, essential to the ontogenetic development of adaptive immunity in that species. surgical removal of one or both of these organs in the newly hatched chicken, followed by sublethal x-irradiation the next day, has permitted recognition of two morphologically distinct cell systems in the "peripheral lymphoid tissues" of the spleen, gut, and other organs, and clear definition of the separate functions of each cell sys ... | 1966 | 5323079 |
| antibody elicited by brucella abortus antigen in vitro: prolonged production by cell suspensions. | 1969 | 5765459 | |
| [a contribution to the differentiation of doubtful brucella abortus agglutination reactions by means of the so-called heat test]. | 1965 | 5891561 | |
| [experiences in bang's disease. results of 2 mass surveys of brucella abortus bang 1960/1 and 1964]. | 1965 | 5893864 | |
| [the results of studying brucella vaccine from the brucella abortus 104-m strain]. | 1965 | 5896075 | |
| [diathelic immunization with brucella abortus]. | 1965 | 5896193 | |
| [level of agglutinating and complement fixing antibodies in guinea pigs infected in different ways with "brucella abortus"]. | 1966 | 5913240 | |
| bovine brucellosis: a study of an adult-vaccinated, brucella-infected herd. i. the effect of brucella abortus infection on fertility. | 1966 | 6007516 | |
| a survey of dairy cattle in south-western victoria showing their serological status for brucella abortus agglutinins. | 1966 | 6008286 | |
| resistance of cattle to brucella abortus following vaccination at two and three months of age. | 1967 | 6067831 | |
| investigation into the usefulness of the non-agglutinogenic brucella abortus adjuvant vaccine duphavac n.a. in the control of bovine brucellosis. | 1967 | 6067939 | |
| interpretation of the brucella abortus serum agglutination test. | 1967 | 6067981 | |
| immunochemical studies on the binding properties of brucella abortus lipopolysaccharides to bovine precipitating antibodies. | the binding properties of brucella abortus smooth lipopolysaccharides (lps) to the precipitating antibodies in the serum of a b. abortus s2308 infected cow were studied by quantitative precipitin and precipitin-inhibition assays. crude b. abortus lipopolysaccharides (clps) and chemical modification products of strains 1119.3, 19 and 2308 (virulent) were used for this study. in the quantitative precipitin assay, 13 micrograms of clps precipitated about 4.0-5.2 micrograms nitrogen of precipitating ... | 1984 | 6083449 |
| ingestion and intracellular survival of brucella abortus in human and bovine polymorphonuclear leukocytes. | bovine polymorphonuclear leukocytes (pmns) were found to be significantly more bactericidal than human pmns against a smooth-intermediate strain of brucella abortus (45/0), whereas there was no difference in bactericidal activity of the two kinds of pmns against a rough strain of b. abortus (45/20). electron microscopy of thin sections of pmns revealed that both strains of b. abortus were readily ingested; however, the extent of degranulation was significantly less than in pmns incubated with an ... | 1984 | 6090315 |
| brucellacidal activity of human and bovine polymorphonuclear leukocyte granule extracts against smooth and rough strains of brucella abortus. | the microbicidal activities of freeze-thaw and high-salt extracts of human and bovine polymorphonuclear leukocyte (pmn) granules were tested against a smooth intermediate strain (45/0) and a rough strain (45/20) of brucella abortus which differ in virulence and survival within pmns. freeze-thaw extracts of human pmn granules were more brucellacidal than high-salt extracts when supplemented with hydrogen peroxide (h2o2) and potassium iodide (ki), whereas the opposite was found with freeze-thaw an ... | 1984 | 6090316 |
| evaluation of lymphocyte blastogenesis for diagnosis of bovine brucellosis. | the lymphocyte blastogenesis test (lbt) was evaluated for its usefulness in the diagnosis of bovine brucellosis. using a cell titration assay which measures the blastogenic response over a range of cell concentrations, peripheral blood lymphocytes (pbl) from various populations of cattle were tested with purified brucella abortus porin proteins and with brucella abortus soluble antigen (basa). cattle tested included groups infected with virulent b. abortus strain 2308, vaccinated with b. abortus ... | 1984 | 6092181 |
| how does brucella abortus infect human beings? | 1983 | 6139535 | |
| milkborne brucella abortus infection. | 1984 | 6140415 | |
| isolation and properties of an rna fraction present in brucella culture supernatants. | the supernatant fluids of batch and continuous cultures of brucella strains contained up to 100 mg/l of soluble rna which could be recovered by precipitation with lysozyme, this rna fraction had many of the properties of ribosomal rna and was single-stranded, sensitive to ribonuclease, with an approximate sedimentation constant of 5s, a molecular weight of about 35000 daltons and an adenine; guanine; cytosine; uracil content of 17.5; 26.5; 33; 23 mol% respectively. rna fractions from lysozyme pr ... | 1980 | 6153668 |
| the effect of synthetic polynucleotides on the intracellular growth of brucella abortus in vitro and in vivo. | 1980 | 6156736 | |
| selective effects of cyclosporin a on functional b cell subsets in the mouse. | cyclosporin a, an immunosuppressive peptide of fungal origin, was believed to selectively affect t lymphocyte functions and to have minimal affects on b lymphocytes. this study shows that, in the mouse, t-dependent b cells and those responding to certain t-independent antigens (so-called ti-1 antigens) are indeed resistant to the drug. however, b cells responsive to other ti antigens (ti-2) are exquisitely sensitive. thus, doses of the drug that completely abrogate responses to dinitrophenylated ... | 1980 | 6159410 |
| role of accessory cells in b cell activation. iii. cellular analysis of primary immune response deficits in cba/n mice: presence of an accessory cell-b cell interaction defect. | the effect of the x-linked cba/n genetic defect on the ability of mice to generate primary responses to thymic-dependent and thymic-independent antigens was assessed by comparing the ability of abnormal (cba/n x dba/2)f1 male mice and normal (dba/2 x cba/n)f1 male mice to generate 2,4,6-trinitrophenyl (tnp)-specific plaque-forming cell responses to tnp-keyhole limpet hemocyanin (klh), tnp-conjugated ficoll (tnp-ficoll), tnp-brucella abortus (ba), and tnp-lipopolysaccharide (lps). the reciprocal ... | 1980 | 6159444 |
| immunochemical characterization of brucella lipopolysaccharides and polysaccharides. | purified lipopolysaccharide (lps) extracted with phenol-water from smooth brucella abortus was hydrolyzed with 1% acetic acid at 100 degrees c. the degraded polysaccharide (ah) released gave reactions of identity with the native polysaccharide hapten (nh) in phenol-water- or trichloroacetic acid-extracted endotoxin preparations of b. abortus and with the polysaccharide (poly b) extracted by trichloroacetic acid from rough b. melitensis strain b115. poly b was present in the soluble cytoplasmic f ... | 1981 | 6163716 |
| the effect of second signals on the induction of b cell tolerance: failure of helper t cells to block tolerance induction. | the effect of carrier-primed helper t (th) cells and t cell-replacing factors on the induction of hapten-specific tolerance in b cells from adult mice has been tested. the 2,4,6-trinitrophenyl conjugate of human gamma-globulin (tnp17hgg) was used as tolerogen in an in vitro tolerance induction system. tolerance was assessed by the subsequent induction of plaque-forming cell responses using tnp-brucella abortus and trinitrophenylated sheep red blood cells (tnp-srbc) plus srbc-primed th cells as t ... | 1981 | 6169534 |
| transfer of memory cells into antigen-pretreated hosts. ii. influence of localized antigen on the migration of specific memory b cells. | following i.v. injection, 2,4,6-trinitrophenol (tnp)-primed memory cells localized in recipient lymph nodes draining a footpad injection of tnp-hemocyanin (tnp-klh) in greater numbers than in contralateral nodes draining a p-azobenzenearsonate-coupled klh injection. such hapten-specific, unilateral memory b cell localization was still observed in immunosuppressed mice when antigen injections were given as long as 4 days prior to the memory cell transfer. the memory cells could be challenged to f ... | 1981 | 6173238 |
| enzyme immunoassay of the antibody response to brucella and yersinia enterocolitica 09 infections in humans. | an enzyme immunoassay, with phenol-water extracted lipopolysaccharide (lps) from brucella abortus as antigen, was used to detect the class-specific antibody response in sera from 173 patients with b. abortus, b. melitensis or b. suis infection. sera from 30 patients with salmonellosis, yersiniosis or tularaemia and from 25 healthy individuals served as controls. the b. abortus lps antigen permitted a safe diagnosis of acute and chronic brucellosis with high igm and rising igg titres in sera coll ... | 1982 | 6174601 |
| the capacity and mechanism of bone marrow antibody formation by thymus-independent antigens. | primary immunization of mice with certain thymus-independent (ti) antigens (i.e., tnp-lps and dnp-ficoll) leads to antibody formation in the bone marrow (bm). tnp-brucella abortus, pneumococcus pneumoniae organisms, and alpha-(1,6) dextran, on the other hand, do not induce a bm antibody-producing plaque-forming cell (pfc) response. this paper deals with the mechanism underlying antibody formation in the bm to tnp-lps and dnp-ficoll. the majority of the bm-localizing pfc induced by tnp-lps are fo ... | 1982 | 6174602 |
| t cell-independent responses to an ir gene-controlled antigen. i. characteristics of the immune response to insulin complexed to brucella abortus. | immune responses by mice to heterologous insulins are controlled by h-2-linked ir genes. antibody responses to insulin are t cell dependent (td), and nonresponder mice fail to make detectable insulin-specific antibodies. to further analyze the role of t cells in regulation of immune responses to insulin, we have developed a method for induction of insulin-specific b cells in the relative absence of t cells. insulin has been chemically coupled to the t cell-independent (ti) organism brucella abor ... | 1982 | 6177760 |
| extraction, separation, and partial characterization of brucella abortus antigens. | brucella abortus isolates strain 19 (a vaccinal strain) and 458 (a virulent field isolate) were analyzed for antigenic differences. whole cell preparations were extracted with detergents, salt solutions, and phenol-acetic acid-water (paw). antigens were separated by starch block electrophoresis and sucrose density gradient centrifugation, serotested, and chemically analyzed. six distinct precipitin lines were observed for the paw extract against hyperimmune bovine antisera. with sucrose density ... | 1982 | 6178324 |
| effect of recent antigen exposure on the functional expression of b cell subpopulations. | we investigated whether the definition of functional b cell subpopulations changes after the exposure of b cells to specific antigen. recent in vivo priming with fluorescein- (fl) coupled t-independent (ti) antigens leads to an augmentation of the subsequent in vitro responses of b cells to fl-coupled ti antigens, including fl-polymerized flagellin, fl-lipopolysaccharide, and fl-brucella abortus, as well as a fl-coupled t-dependent (td) antigen, fl-keyhole limpet hemocyanin (klh). this effect, w ... | 1982 | 6179988 |
| definition of conditions that enable antigen-specific activation of the majority of isolated trinitrophenol-binding b cells. | in an effort to further elucidate the early cellular events in generation of antibody responses, we have determined the requirements for antigen-specific initiation of the g0 to g1 transition by isolated trinitrophenol (tnp) -binding b lymphocytes. tnp-binding cells were isolated from normal b6d2f1 splenocyte populations using hapten affinity fractionation on disulfide-bonded tnp-gelatin-coated plates. populations prepared in this way are greater than or equal to 96% immunoglobulin positive and ... | 1982 | 6184434 |
| the role of the differential complement fixation test, using rough and smooth brucella antigens, in the anamnestic test. | to assess the ability of the differential complement fixation test to distinguish vaccinal reactors from infected cattle, approximately 1,000 heifers were tested by the complement fixation test (cft) using rough and smooth brucella antigens, before the injection of 45/20 vaccine and at 3 and 6 or 10 weeks after vaccination. before vaccination 91.5% of heifers were negative to the rough antigen but 0.6% were positive with high titre (greater than or equal to 128). by 10 weeks after injection of 4 ... | 1983 | 6187324 |
| thymus-independent induction and antigen-dependent recovery of idiotype-specific suppression. | balb/c nude (nu/nu) mice and euthymic (nu/+) littermates were treated as neonates with anti-t15 antibody and challenged at various ages with either a thymus-independent, pc-brucella abortus (pc-ba), or thymus-dependent, pc-keyhole limpet hemocyanin (pc-klh), form of phosphorylcholine (pc). nu/nu mice challenged with pc-klh received klh-primed splenic t cells prior to immunization. neither neonatally anti-idiotype-treated nu/+ nor nu/nu mice responded with the production of t15-positive anti-pc a ... | 1983 | 6187483 |
| functional heterogeneity of memory b lymphocytes: in vivo analysis of td-primed b cells responsive to secondary stimulation with td and ti antigens. | the functional heterogeneity of memory b cells induced by a single determinant, consisting of a decapeptide representing amino acid residues 103-112 of tobacco mosaic virus protein (tmvp), was analyzed. decapeptide specific antibodies were elicited in mice adoptively transferred with tmvp-immune spleen cells when challenged with tmvp, decapeptide conjugated to succinylated human gamma-globulin (shgg), or decapeptide conjugated to brucella abortus (ba). whereas secondary stimulation by either tmv ... | 1983 | 6190934 |
| two stages of b cell memory development expressing differential sensitivity to stimulation with thymus-dependent and thymus-independent antigenic forms. | we have shown that spleen cells specifically primed to the decapeptide determinant (a.a. 103-112) of the thymus-dependent (td) antigen, tobacco mosaic virus protein (tmvp), can be secondarily stimulated to antibody synthesis by either td or ti forms of the decapeptide. further, td- and ti-responsive memory b cells consisted of overlapping populations which were indistinguishable by the specificity and isotype composition of the antibodies which they synthesized. in the present study, two functio ... | 1983 | 6190935 |
| antitumor activity of living or killed brucella: modification of the non-specific cytotoxic effector cells. | at various times after injection of living or killed smooth (s) or rough (r) brucella abortus mice received a graft of the semi-allogenic el4 lymphoma and their survival was studied. in parallel, the nk activity of spleen and peritoneal cells, the level of serum interferon (ifn), and the cytotoxic activity of peritoneal macrophages were investigated. protection against the lymphoma lasted longer after injection of r organisms than after s. the parallelism between the in vivo resistance to el4 ly ... | 1983 | 6191859 |
| idiotype profile of an immune response. ii. reversal of the relative dominance of major and minor cross-reactive idiotypes in arsonate-specific t-independent responses. | two different cross-reactive idiotype (cri) groups are distinguishable in the ab response of a/j mice to the p-azobenzenearsonate (aba) hapten: cria and crim. these two groups showed distinct patterns of relative dominance in the ensuing response depending on whether the inducing ag was a t cell-dependent (td) form of aba, such as aba-klh or aba-cgg, or a t-independent type 1 (ti-1) form, such as aba-brucella abortus or aba-lipopolysaccharide (lps), and on whether the response was elicited in vi ... | 1983 | 6193221 |
| a novel role for macrophages: the ability of macrophages to tolerize b cells. | we investigated the ability of macrophages (m phi) to present the tolerogen fluoresceinated sheep gamma-globulin (fl-sgg) to b cells. m phi pulsed with fl-sgg or murine fl-igg2 were able to tolerize normal spleen b cells specifically as assessed by the plaque-forming cell (pfc) response to the antigens fl-brucella abortus (fl-bra) and fl-polymerized flagellin (fl-pol). tolerance was not induced when m phi were pulsed with a variety of other fl antigens or the synthetic tolerogen fl-d-glutamic ac ... | 1983 | 6195250 |
| defective b cell tolerance induction in new zealand black mice. i. macrophage independence and comparison with other autoimmune strains. | b cell unresponsiveness was examined in vitro by using spleen cells from autoimmune nzb, bxsb/mp male, mrl/mp-ipr/ipr (mrl/l), and control mice, and the tolerogen trinitrophenyl human gamma-globulin (tnp-hgg). the b cell subset responsive to tnp-brucella abortus in each autoimmune and control strain that was tested was highly susceptible to tolerance induction with the use of high epitope density conjugates (tnp30hgg and tnp32hgg). when a tolerogen with a lower epitope density was used (tnp7hgg) ... | 1983 | 6196392 |
| a reappraisal of "t-independent" antigens. i. effect of lymphokines on the response of single adult hapten-specific b lymphocytes. | four supposedly t-independent antigens, fluorescein (flu)-e. coli lipopolysaccharide (lps), flu-brucella abortus (flu-ba), flu-ficoll, and flu-polymerized flagellin (flu-pol), were tested for their capacity to stimulate b cell proliferation and antibody formation. single, isolated flu-specific adult murine splenic b lymphocytes were used as the unequivocal target cell in 10-microliter cultures unsupported by accessory, feeder or filler cells. the stimulatory capacity of four supposed mitogens, l ... | 1984 | 6199407 |
| a reappraisal of "t-independent" antigens. ii. studies on single, hapten-specific b cells from neonatal cba/h or cba/n mice fail to support classification into ti-1 and ti-2 categories. | spleen cells from adult cba/h or cba/n mice, or from neonatal cba/h mice, were fractionated on thin layers of fluorescein (flu)-gelatin to yield flu-specific b lymphocytes. a single cell, or small numbers ranging from 1 to 10, were cultured in 10-microliter microcultures together with various antigens and mitogens. the results were compared with those of bulk culture or limiting dilution cultures supported by thymus filler cells. b cell growth and differentiation-promoting conditioned media (bgd ... | 1984 | 6199408 |
| production of auto-anti-idiotypic antibody during the normal immune response. ix. characteristics of the auto-anti-idiotype antibody and its production. | using hapten-reversible inhibition of plaque formation as an assay for auto-anti-idiotype antibody (anti-id) and as a means for following idiotype (id) expression, we have obtained evidence that following immunization with trinitrophenyl (tnp) conjugates (a) there are differences in id expression in the anti-tnp antibody response to different tnp conjugates although there is some overlap; (b) different strains, although showing some differences in id expression, tend to produce cross-reactive id ... | 1984 | 6201287 |
| h-2 control of polyadenylated mrna production in the spleen of mice injected with brucella antigens. | rna was extracted from the splenocytes of brucella abortus antigen stimulated mice and of control mice. the proportion of chromatographically separated polyadenylated 11.2s mrna, was determined. with the technique used, only stimulated mice exhibited significant amounts of this rna species. the highest level was reached 1 day after the stimulation, and the decay from this level presented an oscillatory form during the 4 weeks following the injection. in two different genetic backgrounds, h-2b mi ... | 1984 | 6202799 |
| monoclonal antibodies to brucella surface antigens associated with the smooth lipopolysaccharide complex. | hybridomas producing antibodies to determinants associated with the lipopolysaccharide (lps) of brucella abortus and b melitensis were obtained by polyethylene glycol fusion of the sp2/0 myeloma cell line with b lymphocytes harvested from a sprague-dawley-derived rat previously immunized with whole b abortus strain 1119 organisms. two clones, bru38 and bru28 , were selected for their ability to react with whole b abortus organisms and purified smooth-lps ( f5p ). the bru38 monoclonal antibodies ... | 1984 | 6203443 |
| characterization of anti-idiotypic suppressor t cells (tsid) induced after antigen priming. | the induction and fine specificity of idiotype-specific suppressor t cells (tsid) were studied. spleen cells from c57bl/6 mice, immunized 4 wk previously with np-klh, failed to express npb3 idiotype-bearing pfc when challenged in vitro with np-ficoll or np-brucella abortus. after treatment of np-primed responder cultures with anti-thy-1.2 anti-serum + c, npb idiotype-bearing b cells could be detected. this b cell subset was preferentially suppressed by the addition of t cells from np-primed mice ... | 1984 | 6205068 |
| size-dependent b lymphocyte subpopulations: relationship of cell volume to surface phenotype, cell cycle, proliferative response, and requirements for antibody production to tnp-ficoll and tnp-ba. | murine splenic b lymphocytes were separated into size-dependent subpopulations by using counterflow centrifugation. spleen cells were rigorously depleted of t lymphocytes to yield a population of cells that were greater than 90% surface immunoglobulin (ig)-positive and that had a mean cell volume of 136.6 +/- 3.3 microns. from this population, five fractions of cells were obtained with mean cell volumes that ranged from 115.8 +/- 3.7 microns in fraction 1 to 168.0 +/- 6 microns in fraction 5. th ... | 1984 | 6207226 |
| insulin-specific tolerance induction. ii. tolerant helper t cells can be rescued by insulin complexed to brucella abortus. | murine antibody responses to heterologous insulins are under h-2-linked immune response (ir) gene control. we previously demonstrated that the immune response to insulin in freund's complete adjuvant (cfa) can be specifically inhibited by prior injection of soluble insulin i.v. unresponsiveness requires at least 4 days after i.v. injection to develop, and once induced, it lasts 4 wk or more. unresponsiveness is caused by t cell, but not b cell, tolerance; furthermore, we have been unable to demo ... | 1984 | 6208264 |
| protection of chickens by ribosomal vaccines from pasteurella multocida: dependence on homologous lipopolysaccharide. | chickens were protected against fowl cholera by ribosomal vaccines prepared from noncapsulated pasteurella multocida. passive hemagglutination (pha) titers to lipopolysaccharide (lps) and the degree of protection conferred by ribosomal vaccines were diminished or abolished when ribosomes were chromatographed on an immunoadsorbent column. addition of subimmunogenic amounts of serotype 1 (homologous) lps to highly purified ribosomes resulted in vaccines that protected against challenge exposure an ... | 1984 | 6208828 |
| effectiveness of various therapeutic regimens for bovine brucellosis. | various chemotherapeutic regimens were evaluated in 48 culture-positive dairy cows. cessation of shedding of brucella abortus from udder secretions and absence in selected tissues at necropsy were criteria of success. a combination of a long-acting oxytetracycline and streptomycin eliminated brucella in 10 of 14 (71.4%) cows. two cows that were retreated with the same regimen also became culture-negative. other treatment regimens, including the use of liposome-encapsulated antibiotics, were less ... | 1984 | 6208830 |
| mechanism of lipopolysaccharide-induced immunosuppression: immunological activity of b cell subsets responding to t-dependent or t-independent antigens in lipopolysaccharide-preinjected mice. | spleen cells from mice preinjected with high doses of bacterial lipopolysaccharide did not generate anti-trinitrophenyl (tnp) plaque-forming cells in vitro to the t-dependent antigen, tnp-sheep erythrocytes, but did generate fully plaque-forming cells to the t-independent antigens, tnp-ficoll and tnp-brucella abortus. the immunological activity of b cells from such lipopolysaccharide-preinjected mice was analyzed in the present study. t cell-depleted spleen cells from mice injected with 30 micro ... | 1984 | 6235192 |
| tolerance, viral shedding, and neoplasia in chickens infected with non-defective reticuloendotheliosis viruses. | chickens inoculated as embryos with non-defective reticuloendotheliosis viruses (ndrevs) generally developed a "tolerant" infection characterized by lack of immunofluorescent antibody and by a viremia that persisted through 93 weeks. chickens inoculated at hatching generally developed a "non-tolerant" infection characterized by antibody development that gradually waned as the chickens aged and by a transient or intermittent viremia. although chickens tolerantly infected with ndrev strain t were ... | 1981 | 6266388 |
| serologic survey for selected microbial agents in mammals from alberta, 1976. | blood samples were taken from humans and several species of free-ranging wild mammals from five different geographic areas of alberta, canada. sera were tested for antibody to eastern equine encephalitis (eee) virus, western equine encephalitis (wee) virus, st. louis encephalitis (sle) virus, powassan (pow) virus, the snowshoe hare (ssh) strain of the california group (cal) of viruses, northway (nor) virus, klamath (kla) virus, infectious bovine rhinotracheitis (ibr) virus, and two bacteria, bru ... | 1981 | 6273600 |
| duration of immunosuppression caused by a vaccine strain of infectious bursal disease virus. | the depression of response of brucella abortus strain 19 caused by an infectious bursal disease vaccine virus given to chicks at one day old was shown to persist for four weeks. histological examination of the bursa of fabricius showed a gradual repopulation by bursal lymphocytes, after initial damage, concomitant with the development of a humoral response. | 1982 | 6283614 |
| immunocompetence of chickens during early and tumorigenic stages of rous-associated virus-1 infection. | a study was designed to determine the effects of congenital infection with the rous-associated virus-1 (rav-1) on the immune function chickens during the early and late tumorigenic stages of infection. in another experiment, the effects of niridazole on the immune competence and the tumor incidence in chickens congenitally infected with rav-1 were studied. lymphocyte stimulation by phytohemagglutinin, the phytohemagglutinin skin test, the response to immunization with sheep erythrocytes and bruc ... | 1982 | 6290392 |
| fatty acid analysis for differentiation or bordetella and brucella species. | the fatty acid composition of bordetella pertussis (13 strains), b. parapertussis (3 strains), b. bronchiseptica (6 strains), brucella abortus (6 strains), b. melitensis (4 strains) and b. suis (5 strains) was determined. both genera contained straight-chain saturated and mono-unsaturated acids as well as cyclopropane substituted isomers, but the overall differences between the two genera were distinct. the brucella species contained exclusively c16 to c19 acids. minor amounts of hydroxylated ac ... | 1982 | 6293245 |
| [hepatic granulomatosis and positive serodiagnosis of yersinia infections]. | four cases of hepatic granulomatosis with positive yersinia serologic tests are reported. in one case, the wright serodiagnostic tests were also positive. in the three other cases, no other likely etiology was demonstrated. the authors recall the main causes of hepatic granulomatosis, diagnostic criteria of yersiniosis infections, and the possible occurrence of simultaneous positive serodiagnostic tests for yersinia enterocolitica type 9 and brucella abortus. | 1983 | 6314525 |
| immune response to porin in cattle immunized with whole cell, outer membrane, and outer membrane protein antigens of brucella abortus combined with trehalose dimycolate and muramyl dipeptide adjuvants. | the immune response of cattle to nonliving vaccines derived from brucella abortus rough strain 45/20 was studied. vaccines contained trehalose dimycolate and a derivative of muramyl dipeptide. n-acetylmuramyl-l-alpha-aminobutyryl-d-isoglutamine. a factorial experiment was designed to test the effects of type of antigen, quantity of antigen, and quantity of mineral oil on the immune response to porin. muramyl dipeptide was kept constant at 5 mg per dose, and 1 part of trehalose dimycolate was inc ... | 1983 | 6315592 |
| pathogenesis and cellular immunity in experimental murine brucellosis. | infection of mice with brucella abortus strain 19 provides a most useful and interesting model in which to study chronic infection with intracellular bacteria. most strains of mice develop a chronic infection. however, certain strains are better able to handle their infection. long term bone marrow chimeras showed this to be due to bone marrow derived cells, rather than host physiology, although whether it is due t or b lymphocytes, macrophages or polymorphs is yet to be determined. in vitro tre ... | 1984 | 6333362 |
| a requirement for nonspecific t cell factors in antibody responses to "t cell independent" antigens. | using murine splenic b cell preparations depleted of macrophages and rigorously depleted of t cells, we studied the role of nonspecific helper factors in in vitro antibody responses to t cell-independent (ti) type 1 and type 2 antigens. tnp-lipopolysaccharide, tnp-brucella abortus, and dnp-liposomes containing lipid a were chosen as examples of ti type 1 antigens. dnp-ficoll and dnp-liposomes without lipid a were chosen as ti type 2 antigens. only the type 1 antigens were able to elicit signific ... | 1983 | 6336770 |
| selective suppression by auto-anti-idiotypic antibody of b-cell idiotype repertoires generated after stimulation with the same hapten on t-dependent and t-independent carriers. | in the present study, we investigated whether auto-anti-idiotypic antibody in the immune sera from old mice could recognize antitrinitrophenyl (tnp) plaque-forming cells (pfc) generated after stimulation with the t-dependent and t-independent forms of the hapten, tnp. young and old c57bl/6j male mice were immunized with a variety of t-dependent (tnp-bovine gamma-globulin, tnp-bgg; tnp-keyhole limpet hemocyanin, tnp-klh; ovalbumin, ova; bovine serum albumin, bsa; bgg) and t-independent (tnp-bruce ... | 1983 | 6360382 |
| opposite effects of total lymphoid irradiation on t cell-dependent and t cell-independent antibody responses. | the effect of total lymphoid irradiation (tli) on the primary antibody response to the dinitrophenylated heterologous protein, keyhole limpet hemocyanin (dnp-klh), in complete freund's adjuvant (cfa), and to the trinitrophenylated polysaccharide antigen, brucella abortus (tnp-ba), was studied in balb/c mice. the antibody response to both antigens was diminished in comparison with nonirradiated mice when antigens were injected within 3 days after tli. when the mice were immunized 30 days after co ... | 1984 | 6361136 |
| physiology of igd. iv. enhancement of antibody production in mice bearing igd-secreting plasmacytomas. | immune responses to trinitrophenylated hemocyanin (tnp-klh), ficoll (tnp-ficoll), and brucella abortus (tnp-ba) were examined in balb/c mice bearing subcutaneous transplants of tepc-1017 and tepc-1033, the two known igd-secreting balb/c plasmacytomas. both primary and secondary 19s and 7s splenic plaque-forming cell (pfc) responses in spleen to intravenously injected tnp-klh were enhanced three to fivefold. primary responses to tnp-ficoll were 1.5-2 times higher than in control mice (particularl ... | 1984 | 6363593 |
| differential susceptibility of rodent malaria parasites to nonspecific immunity. | nonspecific immunity to rodent malaria parasites can be produced by immunising mice with heterologous parasites or pretreating them with bcg, corynebacterium parvum or brucella abortus. nonspecific immunity is easily produced against plasmodium vinckei but not p. berghei. this is due to the difference between the cells occupied by the parasites, young red blood cells in the case of p. berghei and mature red blood cells in the case of p. vinckei. | 1983 | 6370475 |
| monoclonal antibody-based enzyme-linked immunosorbent assays for identification and serotyping of vibrio cholerae o1. | monoclonal antibodies directed against o-specific antigens of vibrio cholerae o1 lipopolysaccharide were used in two different enzyme-linked immunosorbent assays (elisas), designed for identification and serotyping of v. cholerae o1. in the sandwich elisa, a monoclonal antibody against the group-specific antigen was used as capture antibody, whereas peroxidase-conjugated monoclonal antibodies directed against group- and type-specific antigens were used as the second antibodies. monoclonal antibo ... | 1984 | 6394621 |
| isolation, purification, and partial characterization of brucella abortus matrix protein. | peptidoglycan sacculi with peptidoglycan-associated proteins were prepared from cell envelopes of brucella abortus by extraction with sodium dodecyl sulfate (sds) at 50 degrees c. on extraction of these preparations with sds at 100 degrees c, a protein was obtained whose removal from peptidoglycan was confirmed by electron microscopy. incubation of the 50 degrees c sds-extracted cell envelopes with 50 mm mgcl2 in sds-2-beta-mercaptoethanol at 37 degrees c also extracted the protein, along with l ... | 1983 | 6401696 |
| comparison of standard tube and microagglutination techniques for determining brucella antibodies. | a microagglutination method for determining the agglutinating and "blocking' antibodies to brucella abortus is described. a collection of sera from healthy blood donors in two rural areas of new zealand were tested by the microagglutination methods and the standard methods in tube. the results are compared and show that where discrepancies occur, these are due to the microagglutination methods being more sensitive. it is concluded that these are suitable methods for screening populations. | 1983 | 6401775 |