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[biohazard in clinical laboratories in japan].this survey of occupationally acquired infections in clinical laboratory workers was made by questionnaires to 306 hospitals in which 698 doctors and 8654 technicians worked. there were 177 probable infections during the previous decade (1979-88). in both doctors and technicians annual incidence rate of infection was 0.2% on an average. these included 77 cases of tuberculosis, 59 cases of hbv hepatitis, 24 cases of non-a, non-b hepatitis, 6 cases of rubella, 5 cases of hav hepatitis, 2 cases of ...19912066604
observations made on strains of campylobacter spp. isolated in 1989 in northern italy.over a 12-month period (1989), 192 thermophilic campylobacters (190 from stool and two from blood specimens) were isolated. all the strains were identified as far as species and biotype was concerned and 89 were also serotyped according to the lior method. c. jejuni (90.10%) was the species most frequently isolated (mainly biotype i) while the most frequent serogroup was lio 4. erythromycin-resistance was found in only 15 strains, but while it was very low for the species c. jejuni (1.16%), it w ...19912067413
chronic active gastritis after eradication of campylobacter (now: helicobacter) pylori. results of a medium term follow-up study.11 patients with c.p.-associated chronic gastritis underwent a triple therapy with bismuth-subsalicylate, amoxicillin susp. and metronidazole. the c.p.-status and the inflammation parameters "polymorphonuclear leucocytes in the epithelium". "polymorphonuclear leucocytes in the lamina propria", "lympho-plasmacellular infiltrate in the lamina propria", "congestion and edema of the lamina propria" and "inflammatory alteration of the epithelium" were determined semi-quantitatively in semi-thin-secti ...19912068004
helicobacter pylori in children.the clinical manifestations of helicobacter pylori (formerly campylobacter pylori) infection in children overlap with those encountered in adults but there are some important differences. the aim of this review is to describe the range of manifestations of this infection in children, together with means of diagnosis and treatment.19912068024
[the humoral and cellular immunity indices of patients with long-term nonhealing stomach ulcers with the presence of campylobacter pylori].the authors relate the data on humoral and cellular immunity in patients with long nonhealing gastric ulcers. it has been shown that if these patients have campylobacter pylori, there are no specific differences in the response of the immune system. during cicatrization, positive changes can be seen, namely an increase of t helper and decrease of suppressor activity of t lymphocytes together with a decline of the content of iga and igg.19912068663
isolation of a urease positive thermophilic variant of campylobacter lari from a patient with urinary tract infection.an unusual campylobacter strain, a urease positive thermophilic variant of campylobacter lari, was isolated from the urine of a patient with urinary tract infection who was hospitalized because of cirrhosis and haemorrhage. the strain was isolated from urine specimens on three separate occasions. a significant serological response to the organism was also detected. this is the first documented case of extra-intestinal infection due to this group of organisms.19902073901
in vitro activity of tosufloxacin against bacterial enteric pathogens.the in vitro activity of tosufloxacin (a-61827), a new quinolone antibiotic, was compared with that of four other quinolones against 162 bacterial enteric pathogens. susceptibility testing was performed by using an agar dilution method. the minimal inhibitory concentration (mic) was defined as the lowest concentration of antibiotic without visible growth. tosufloxacin was the most active agent against campylobacter jejuni and c. coli, and ciprofloxacin was the most active agent against salmonell ...19902076595
helicobacter pylori and gastric carcinoma.a retrospective study was performed on gastric carcinomas to establish the prevalence of helicobacter pylori infection in gastric epithelium adjacent to the tumour. a total of 105 carcinomas were studied. the overall prevalence of helicobacter pylori infection was 59%. the prevalence in different age cohorts from patients with gastric carcinoma was compared with that in patients suffering from non-ulcer dyspepsia and, based on serological testing, with that in healthy blood donors. the presence ...19902076886
six enteropathogens isolated from a case of acute gastroenteritis.isolation of six different gastrointestinal pathogens (entamoeba histolytica, plesiomonas shigelloides, campylobacter jejuni and three different salmonella species [salmonella typhimurium, salmonella blockley and salmonella hadar]) in the feces of a german female tourist suffering from acute diarrhoea after a trip to bali.19902076911
[infantile diarrheal diseases in madagascar: bacterial, parasitologic and viral study].from november 1988 to october 1989, an etiological study showed off the prevalence and the part of several enteropathogen agents which are not yet studied in madagascar. 1,523 stool's samples from 884 children with diarrhea and 639 children without diarrhea from 0 to 14 years old have been investigated. a bacterial, parasitical or viral etiology was found from 36.3% of diarrheic children and 11.2% of healthy children. the three agents the most frequently identified from children with diarrhea ar ...19902078082
[evaluation of the prevalence of the main enteropathogenic agents in the west imerina region: manarintsoa].for a 288 children's population aged from 5 to 14 years old and being school attendents in the village of manarintsoa, we have systematically investigated 60% of them in may 1988 and january 1989. bacterial, parasitical and viral study of these stools showed off an important number of a. dispar and e. coli strains which have special phenotypes characters. the prevalence of campylobacter and ascaris lumbricoïdes is higher in this rural zone than in tananarive. two poliovirus of type 2 have been i ...19902078083
identification of campylobacter jejuni and campylobacter coli from waste water by sds-disc-page of whole cell protein.analysis of the electrophoretic profiles of whole cell proteins of campylobacter strains from waste water was used to differentiate c. jejuni from c. coli. the results show, that biotyping of campylobacters from waste water may results in a false species differentiation. some of the campylobacter strains originally typed as c. coli, were proved to be atypical c. jejuni strains. therefore, in addition to biotyping and serotyping, electrophoretic typing is recommended to ensure correct identificat ...19902080967
[evaluation of a latex agglutination method for the rapid identification of campylobacter jejuni/coli].a latex agglutination assay was developed to identify campylobacter jejuni and campylobacter coli. we evaluated the specificity, reproducibility and utility of the assay for clinical use and the following results were obtained. 1) to prepare standardized antigen, bacterial cells must be suspended to a density of 1 to 5 mcfarland unit, and heated at 121 degrees c for 10 to 30 min. 2) bacterial cells may be suspended either in the solution provided with the kit, or in physiological saline, without ...19902082036
phallus-inflammation of ganders: clinical observations and comparative bacteriological examinations of healthy and altered organs.the examination of acute or chronically altered phallus-tissues of ganders revealed microorganisms belonging to the genera mycoplasma, bacteroides, clostridium, streptococcus, micrococcus, staphylococcus, lactobacillus, corynebacterium, escherichia, campylobacter, proteus, pseudomonas and candida and to the family pasteurellaceae, isolated in different frequencies. bacteriologic examinations of the phallus-tissues and cloacal mucous membranes of healthy juvenile ganders showed microorganisms of ...19902082630
it is h pylori now. 19902084105
[campylobacter pylori in gastroduodenal pathology]. 19902084340
[value of microbiologic studies for diagnosis of post-enteritis reactive arthritis].reactive arthritis may develop within a period of some days until upto 3 weeks after infections with yersinia enterocolitica, yersinia pseudotuberculosis, campylobacter jejuni/coli, shigella and salmonella. intestinal infections with klebsiella pneumoniae, clostridium perfringens, clostridium difficile, cryptosporidium, strongyloides stercoralis, taenia saginata and schistosoma mansoni are, in some cases, considered to be responsible for reactive arthritis. detection of pathogenic bacteria in fe ...19902085058
synergism between clindamycin and colloidal bismuth subcitrate against helicobacter (campylobacter) pylori in vitro.a combination of clindamycin and colloidal bismuth subcitrate was evaluated for synergistic inhibition of helicobacter pylori employing the agar dilution method. a total of 47 clinical isolates of helicobacter pylori were examined. synergistic interaction was observed in 36%, additive behaviour in 64% of the strains. no antagonism could be detected.19902085373
campylobacter peritonitis in a capd patient. 19902085566
campylobacter jejuni peritonitis during chronic ambulatory peritoneal dialysis (capd).a patient treated with chronic ambulatory peritoneal dialysis developed recurrent peritonitis. during a fourth episode, campylobacter jejuni was cultured from the dialysate. she responded well to streptokinase and imipenem.19902085604
[enzyme-linked immunosorbent assay for the serodiagnosis of campylobacter pylori infection].antibody activities to campylobacter pylori in serum were estimated by an enzyme-linked immunosorbent assay (elisa) to crude antigens, prepared by sonication of whole organisms obtained from bacterial culture in 100 patients with chronic gastritis. significantly raised serum igg antibody activities to c. pylori was found in colonised patients with gastritis, especially in patients with active gastritis. high activities were also found beyond the age of 30. in 6 patients cleared of c. pylori with ...19902086012
[reactive arthritis].the term reactive arthritis was introduced to describe an acute non-purulent arthritis complicating an infection elsewhere in the body. reactive arthritis can also be classified into hla-b27 associated and non-associated forms. rheumatic fever is an example of the hla-b27 non-associated forms with genetic factors other than hla-b27 involved. hla-b27 associated reactive arthritis includes enteric, urogenic and idiopathic arthritides. the bacteria known to trigger post-enteritic reactive arthritis ...19902087418
campylobacter fetus subspecies fetus meningitis with chronic alcoholism and diabetes mellitus.a case of campylobacter fetus subspecies fetus meningitis is reported. the patient had underlying diseases, namely chronic alcoholism and diabetes mellitus. the infection did not respond to piperacillin and cefotaxime, but did respond to ampicillin and moxalactam. the patient was discharged on the 33rd hospital day showing no neurological deficit complications, and has remained free of recurrent disease for one month after the discontinuation of therapy.19902089181
[characteristics of helicobacter (campylobacter) pylori infection in our field].the presence of helicobacter pylori in the stomach in patients with clinical signs of gastroduodenal disease and its possible relationship with several clinical, endoscopic and anatomopathologic characteristics of the illness were prospectively evaluated in 359 patients. h. pylori was isolated from 388 samples in 216 patients (60% of colonization). in patients with endoscopic and, or, anatomopathologic evidence of gastroduodenal alterations the microorganism was isolated in 67% of the instances ...19902089203
[bacteremia caused by campylobacter fetus ssp fetus in a patient with liver cirrhosis]. 19902090246
[bacteremia caused by campylobacter fetus ssp fetus in an hiv-positive patient with liver cirrhosis]. 19902090247
[campylobacteriosis]. 19902090491
aetiology of bacterial diarrhoea in north indian children with special reference to campylobacter jejuni.a total of 184 cases of acute diarrhoea, in patients aged 1-36 months, were studied clinicobacteriologically. enteropathogens were isolated in 22.28% cases. campylobacter jejuni was isolated from 2.17% cases only. salmonellae were isolated from 7.6% cases and 64.28% of them were s typhimurium. more than 75% of all isolates of salmonellae, shigellae, esch coli and campylobacter jejuni were sensitive to gentamicin and cephaloridine. secondary lactose intolerance was noted in 6.52% cases only.19902090681
[detection of campylobacter pylori in gastric mucosa--relation to gastroscopy findings and degree of gastritis].in 862 unselected gastroscopies we could confirm the high percentage of campylobacter pylori infection in the antral gastric mucosa strongly associated with chronic superficial gastritis and active inflammatory signs. significant correlation with age concerned the normal findings only. in patients with duodenal ulcers we found in 91% gastritis and in 88% campylobacter pylori in antral mucosa. a small group with duodenal ulcers was campylobacter pylori-negative as well as without gastritis signs. ...19902091669
comparison of four different methods for detection of helicobacter pylori from gastric biopsies.helicobacter pylori are commonly found colonizing the gastric mucosa of different populations. its presence may be important in the pathogenesis of gastritis and peptic ulcer disease. the detection rates vary widely depending upon the diagnostic methods applied. in this study, multiple gastric biopsies were taken from the fundal and antral mucosa of 25 patients during endoscopy. in one patient, the procedure was repeated about two months after the initial biopsy. a total of 52 sets of specimens ...19902091902
relative merits of various rapid biopsy urease tests for diagnosis of helicobacter pylori (campylobacter pylori).three rapid urease tests, i.e., liquid urea broth containing phenol red as indicator, liquid urea broth containing bromothymol blue as indicator and clo gel were compared in 109 patients of dyspepsia for the diagnosis of campylobacter pylori (helicobacter pylori) infection. mean time taken for positive reaction in liquid broth with phenol was 3 minutes (range 0.6 to 5.3 minutes) with bromothymol blue was 3.5 minutes (range 0.4 to 5.5 minutes) while with clo gel it was 101 minutes (range 11-261 m ...19902092020
unusual features of helicobacter (campylobacter) pylori--associated gastritis in india. a study of 200 cases.helicobacter (campylobacter) pylori has been cultured from the antral biopsies of 85-90% of patients of gastritis, gastric ulcer and duodenal ulcer at different centres. studies conducted all over the world have firmly implicated this organism in the aetiology of active superficial gastritis and recurrences of duodenal ulcer. two hundred patients with upper abdominal pain, distension, vomiting and/or haemetemesis were subjected to ogd scopy. in 163 of these patients there was endoscopic evidence ...19902092022
[a 3-year follow-up study of the incidence of campylobacteriosis in a pediatric population].the authors investigated the incidence of campylobacteriosis in the population of five paediatric health communities of the jihlava policlinic (5831 children) for a period of three years. a total of 2408 specimens faeces from 1501 subjects were examined. campylobacter jejuni was detected in 151 subjects (10.1%), salmonella sp. in 47 (3.1%), shigella sp. in 18 (1.2%), yersinia enterocolitica in 12 (0.8%) and enteropathogenic escherichia coli in five subjects (0.3%). the total incidence of campylo ...19902092913
[biochemical, physiological, and enzymatic study of 78 strains of helicobacter (campylobacter) pylori isolated from gastroduodenal biopsies].a biochemical, physiological and enzymatic study of 78 c. pylori strains isolated from gastroduodenal biopsies is reported. all strains were positive in the oxidase, catalase and urease tests. 97.4% produced sh2 in the lead acetate band and 79.4% showed beta-hemolytic activity in sheep blood agar. in the antibiotic selection tests, c. pylori was characterized to be resistant to nalidixic acid and sensitive to cefalotin . the enzymatic study demonstrated the presence of acid and alkaline phosphat ...19902094388
laboratory findings associated with abomasal ulcers/tympany in range calves.the etiology of abomasal ulcers/tympany was investigated in 48 animals from 36 ranches in wyoming and nebraska. results indicate that subclinical trace mineral deficiencies of copper and/or selenium exist in the range cattle in west central nebraska and wyoming. etiological agents most frequently incriminated by bacteriologic cultures and/or histopathic examination were clostridium perfringens and campylobacter species. histopathologic evaluation of abomasums revealed 31 of 38 cases contained ab ...19902094446
isolation of campylobacter coli from a proliferative intestinal lesion in a ferret. 19902094455
[comparative study of campylobacter pyloridis and campylobacter jejuni under electron microscope].specimens were made by preparing the ultrathin sections after negative staining and ruthenium red staining. the specimens were observed under electron microscope. results revealed that the shapes of campylobacter pyloridis (cp) and c. jejuni (cj) were similar, but distinctive differences in their structures were observed. cp had one to five sheathed flagella at one end with bulbous tips. cj had only one unsheathed flagellum on each end without a bulbous tip. the cell body of cp was longer than t ...19902094635
[primary study on pathogenesis of campylobacter jejuni enteritis].the invasiveness and heat-labile enterotoxin of 11 human strains of c. jejuni were detected in nih mice inoculated with the c. jejuni strains; the methods of plate immunological hemolysin test and spa-coagglutination test were employed. ten strains of the bacteria invaded the intestine; 7 strains (45.46%) were positive in spa-coagglutination test; and 4 (36.36%) produced enterotoxin. c. jejuni in chengdu area possess multiple pathogenicity, e.g invasiveness and heat-labile enterotoxin, but we di ...19902094644
eradication of campylobacter pylori and recurrence of duodenal ulcer. a six-month follow-up study.fifty-four patients were studied, 41 men and 13 woman, aged 26-76 years, suffering from duodenal ulcer that healed 4-8 weeks after colloidal bismuth subcitrate therapy (360 mgr twice a day). all patients underwent endoscopy before treatment, 4-8 weeks and 6 months later. biopsy was taken for campylobacter pylori (c.p.) detection by urease test. all patients were c.p. positive initially. the organisms were eradicated in 36/54 patients (67%) at the end of therapy. ulcer healing was obtained after ...19902095099
adherence of helicobacter pylori cells and their surface components to hela cell membranes.four helicobacter pylori strains were used to develop in vitro methods to assess adherence to hela cells. using direct detection by microscopy, adhesion scores increased with the initial bacteria-to-cell ratio. the urease method assessed h. pylori bound to hela cells by their urease activity. the percentage of the original inoculum adhering to hela cells remained constant for initial ratios from 10(2) to 10(5) bacteria per cell. an elisa using anti-h. pylori serum assessed whole bacteria or comp ...19902097496
[therapeutic implications of the identification of species within the genus campylobacter]. 19902098133
immuno-histochemical and -cytochemical evidence suggesting the presence of campylobacter jejuni and campylobacter coli in cases of porcine intestinal adenomatosis.antisera against a number of campylobacter species were used in immuno-histochemical and -cytochemical studies on cases of porcine intestinal adenomatosis. avidin-biotin-complex (abc) and streptavidin immunoperoxidase methods were used on formalin-fixed, paraffin-embedded and frozen sections. protein a gold method was used on formaldehyde fixed and frozen sections for immuno-cytochemistry. the antisera used were raised in rabbits by subcutaneous or intravenous injection of living or formalin tre ...19902099622
[pericarditis caused by campylobacter fetus fetus]. 19902099869
the incidence and epidemiological significance of campylobacter jejuni/coli serotypes in romania.the distribution per serotypes of 401 campylobacter strains was studied by lior's procedure, 241 strains being identified as c. jejuni (60.1%) and 160 as c. coli (39.9%): out of these 329 were of human origin (82%), including 225 c. jejuni and 104 c. coli, and 72 were isolated from fowls and swine (16 c. jejuni, 56 c. coli). out of the total of 401 strains, 363 (90.5%) were typable. among human strains, 32 serotypes were identified, more frequently encountered being (in a decreasing order) 29, 4 ...19902101205
management of acute diarrhoea.before 1970, laboratory staff could not only identify the causative organism of acute diarrhea in 20% of cases, but in 1990, they could identify it in 80% of cases. these organisms are either bacteria, virus, or parasites. the bacteria include enterotoxigenic bacteria (vibrio cholerae, enterotoxigenic escherichia coli, clostridium perfringens, and staphylococcus aureus) and enteroinvasive bacteria (campylobacter jejuni, c. coli, and salmonella and shigella species). the leading cause of death ...19902101385
etiological agents of diarrhoea.two decades of research have established newer pathogens and techniques in establishing several organisms of diarrhoeal diseases as aetiological agents. it is now possible to detect an agent in 80% of the situation of diarrhoea in a standard laboratory. the brief review describes the list of pathogens, their diagnostic techniques with short description on clinical and epidemiological status.19902101388
detection of helicobacter pylori from endoscopic biopsies and the biochemical characteristics of these isolates.helicobacter pylori (formerly campylobacter pylori) has been recently described as a gastritis-associated bacterium. we examined endoscopic biopsies of 100 patients with dyspepsia and found h. pylori in the gastric antrum of 34 (34%) by either culture, urease tests and/or histology. thirty-one out of 41 patients (75.6%) confirmed to have chronic active gastritis histologically had h. pylori in their gastric antrum compared to 3 out of 59 patients (5.1%) with dyspepsia but normal histology (p les ...19902102969
campylobacter pylori infection: experience in a multiracial population.over a 15-month period, 399 patients with dyspepsia were investigated for the presence of campylobacter pylori infection. half of the patients (50.6%) had campylobacter organisms in the antrum of the stomach. c. pylori was found in 96.1% of patients with histological changes of chronic active gastritis in the antrum. of patients with duodenal and gastric ulcers, 87.8% and 87.5%, respectively, had campylobacter organisms, as did 39.3% of patients with non-ulcer dyspepsia. c. pylori infection was ...19902103410
gastric histology and plasma gastrin response to a meal in patients with duodenal ulcer disease after five years treatment with ranitidine.fifty asymptomatic patients with duodenal ulcer disease, aged 31-82 years, who had received ranitidine maintenance therapy continuously for five or more years without a symptomatic recurrence, were studied. fasting plasma gastrin concentrations were normal (mean 24 pmol/l, s.d. +/- 22) while the post-prandial gastrin response was variable with maximum plasma concentrations ranging from 16 to 309 pmol/l. endoscopy revealed six asymptomatic peptic ulcers. histological examination of gastric biopsi ...19902103756
dosage of colloidal bismuth subcitrate in duodenal ulcer healing and clearance of campylobacter pylori.sixty consecutive patients with endoscopically proven duodenal ulcers were given colloidal bismuth subcitrate tablets either as 120 mg q.d.s. or 240 mg b.d., in a randomized single-blind study. the efficacy of each regimen was determined by endoscopic examination and antral biopsy at 4 weeks; if the ulcer remained unhealed, treatment was continued and endoscopy repeated at 8 weeks. the ulcer-healing efficacy of the two regimens was identical; however, in the four times daily group only 27% remai ...19902104073
[ulcer disease--drug treatment and its limitations]. 19902107066
[campylobacter (helicobacter) pylori-induced cost effectiveness-risk considerations]. 19902107370
travelers' diarrhea among u.s. navy and marine corps personnel during a western pacific deployment.the incidence and etiology of travelers' diarrhea was studied in a crew of 1,914 sailors and marines aboard a u.s. navy ship during a western pacific deployment. questionnaires completed by 301 troops indicated that 52% had at least one episode of diarrhea during the deployment; however, only 5% of the ship's company sought treatment. enterotoxigenic escherichia coli was the most commonly identified pathogen (23%), followed by giardia lamblia (6%), salmonella (3%), rotavirus (2%), and shigella, ...19902107464
listeria isolations from feces of patients with diarrhea and from healthy food handlers.a study was undertaken on the presence and frequency of listeria sp. in feces from 1,000 patients suffering from diarrheal diseases and from 2,000 healthy persons. furthermore, the feces of patients were examined for other well-documented enteropathogens such as campylobacter, salmonella, shigella, staphylococcus aureus, yersinia enterocolitica, protozoa and rotavirus as well as for organisms of questionable enteropathogenic potency such as fungi, i.e. candida. finally, in continuation of previo ...19902110118
microbiological quality and safety of zoo food.two types of commercial products for feeding zoo animals (a frozen meat product, referred to as zoo food, and a dry product, referred to as dry food) were microbiologically examined for spoilage organisms (aerobic, psychrotrophic, coliform, escherichia coli, mold, and yeasts) and pathogens (salmonella spp., listeria monocytogenes, and campylobacter jejuni). levels of microorganisms in frozen ground zoo food were compared with those in frozen ground beef and frozen ground turkey meat. the level o ...19902111115
electrolyte abnormalities associated with diarrhea in rhesus monkeys: 100 cases (1986-1987).serum electrolyte values from 100 rhesus monkeys with diarrhea were reviewed. the most frequent finding was hyponatremia (88%), with hypochloremia next most frequently detected (80%). metabolic acidosis was less common (59%) and usually associated with high anion gap values. associations between electrolyte abnormalities and age, housing, or case outcome were not found. bacteriologic culturing was performed on fecal specimens from 90 monkeys. campylobacter coli or c jejuni alone was isolated fro ...19902112122
unsaturated fatty acids and viability of helicobacter (campylobacter) pylori.helicobacter (campylobacter) pylori was found to be sensitive to the toxic effects of an unsaturated fatty acid (arachidonic acid). data are presented that support the hypothesis that exogenous catalase added to basal media enhances the growth of h. pylori by preventing the formation of toxic peroxidation products from long-chain unsaturated fatty acids.19902112559
hydrophobic characterisation of helicobacter (campylobacter) pylori.cell-surface hydrophobicity of helicobacter (formerly campylobacter) pylori was tested by aqueous two-phase partitioning and hydrophobic interaction chromatography. the hydrophobicity of h. pylori greatly exceeded that of campylobacter fetus subsp. fetus, c. jejuni and bacillus subtilis. a partition coefficient (pc) of hydrophobicity in the two-phase system was determined for h. pylori. pc was dependent on ph and the pc value was increased by greater than 20-fold at ph 2.5. lithium cations incre ...19902113100
acp broadsheet 124: may 1990. examination of faeces for bacterial pathogens. 19902115050
response to dr. peterson. 19902115292
role of enterobacteria and hla-b27 in spondyloarthropathies: studies with transgenic mice.several mhc (major histocompatibility complex) genes are associated with increased incidence of disease. the strongest association is between the class i gene, hla-b27, and ankylosing spondylitis (as). hla-b27 is also highly associated with reiter's syndrome. as not all subjects with hla-b27 develop as or reiter's syndrome, environmental factors may have a key role in the pathogenesis of these arthritic diseases. several studies have implicated klebsiella in the development of as, whereas reiter ...19902115764
the 14co2 urea breath test for helicobacter pylori infection. 19902118567
acidity and intestinal bacteria: an in-vitro assessment of the bactericidal activity of hydrochloric acid on intestinal pathogens.the bactericidal activity of hydrochloric acid (hcl) on different intestinal bacteria was evaluated at various ph values and time intervals. the effect of hcl was highest at ph 2 and ph 3 on strains of campylobacter jejuni, aeromonas hydrophila, plesiomonas shigelloides, yersinia enterocolitica, salmonella spp. and shigella spp., producing complete killing after 60 min incubation. however, at higher ph values (ph 4 and above), these organisms not only survived but they also showed evidence of ra ...19902127996
[validity of a score of clinical parameters as a screening method in acute bacterial diarrhea in childhood].we report a prospective study of the cases of acute diarrhea admitted to our pediatric service during one year (n = 172) to elaborate a screening method to identify patients with bacterial diarrhea. a bacterial causative organism was identified in 31.4%. the most common species was salmonella, followed by campylobacter. we evaluated the clinical presentation parameters, looking for differences between the group were a bacterial organism was isolated and in the group where it was not. we designat ...19902129697
[etiological research on acute gastroenteritis in the city of cádiz].we researched the microbial etiology of 5,836 diarrheal processes in outpatients and inpatients at the social security hospital in cádiz, for the purpose of evaluating their microbiological and epidemiological characteristics. bacterial gastroenteritis accounted for 51.6% of the cases, while 31.7% were viral and 16.6% were protozoal in origin. salmonella enterica (35.6%) is the most frequently involved microorganism, especially in the summer, followed very closely by rotavirus (31.7%). the incid ...19902129735
[occurrence of intestinal parasites and campylobacter jejuni in school-age children in krusevac and aleksandrovac]. 19902130015
[occurrence of intestinal parasites and the bacterial flora in school-age children in the sjenica highlands]. 19902130024
morphological study of the gastric antral mucosa colonized by campylobacter pylori.antral biopsies from 56 patients who underwent gastroscopy for upper gastrointestinal symptoms were studied by transmission electron microscopy. campylobacter pylori was demonstrated in specimens from 39 patients. the bacteria were present in 3 of the 8 normal specimens and in 36 of the 48 cases with chronic gastritis. electron microscopy showed that organisms were located deep the mucous layer in intimate relation with the luminar surface of the antral epithelium. no ultra-structural evidence w ...19902131923
the influence of high blood levels of urea on the presence of campylobacter pylori in the stomach: a clinical study.in order to determine whether urea influences the presence of campylobacter pylori (cp) in the stomach, the incidence of this infection was determined in patients with chronic renal failure who received no treatment (group a) or underwent regular hemodialysis (group b). a third group was examined as control. biopsies from the gastric antrum were taken for histological investigation and for cp detection. no significant differences among the 3 groups of patients were found with regards to the inci ...19902131933
the first cases of helicobacter pylori (campylobacter pylori) reported from costa rica.two gastric antrum biopsies of each of 92 consecutive patients undergoing gastroendoscopy were studied. the first biopsy was cultured for helicobacter (campylobacter) pylori. the second specimen was fixed and processed for histopathological analysis. the bacteria were isolated from 34 (81%) of 42 patients with nonspecific chronic gastritis, 19 (90%) of 21 cases of duodenal or gastric peptic ulcer, and from 3 (23%) of 13 normal patients. the overall frequency of isolation was 62 (67%) of the 92 c ...19902134727
chicken carcasses as a source of campylobacter jejuni in belo horizonte, brazil.the presence of campylobacter jejuni was investigated in stool specimens from chicken meat workers and in ready-for-market chicken carcasses from one industrial and nine non industrial slaughters in belo horizonte. in the latter c. jejuni was isolated from 19 (38.0%) of the 50 chicken carcasses and from 2 (13.3%) of the stool specimens obtained from 15 chicken meat workers. in the industrial slaughter it was found in only 1 (2.0%) of the 50 chicken carcasses and it was not isolated from any of t ...19902135487
[can epigastric pain and non-ulcerative dyspepsia in children and adolescents be campylobacter pylori infection?].the authors examined 263 children and adolescents aged 5-20 years who suffered from so-called non-ulcerative dyspepsia or epigastric pain, because of the suspected presence of campylobacter pylori. in 31.9% of the examined subjects direct microbiological methods revealed its presence in the gastroduodenal mucosa. the confidence limit of positive results of campylobacter pylori in the population is between 27.58% and 38.84%, the probability being 95%. the authors proved a mutual correlation betwe ...19902136426
[cytotoxic changes in cell cultures induced by newly isolated strains of campylobacter pylori].suspensions of primocultures of two strains of camplyobacter pylori, isolated from bioptic materials collected from patients with nodular hyperplasia of the pyloric antrum and with gastric ulcers were inoculated to 20-hour confluent or almost confluent cell cultures line vero, mccoy and ma 104 in test tubes and petri dishes. both strains of c. pylori adhered markedly to cell lines vero and mccoy and after 72 hours led to marked morphological changes of the cells. the changes were characterized i ...19902136427
[determination of antibodies to campylobacter pylori in children and adolescents with gastroduodenal disorders].serological examination of antibodies against campylobacter pylori was made in 195 boys and girls aged 9-20 years. in all on account of gastrointestinal complaints endoscopy was indicated. the results of microbiological and histological finding), in particular in the antrum and corpus of the stomach). positive titres antrum and body of the stomach were compared with results of the complement fixation test and the elisa reaction. antibodies against c. pylori were detected in 64 of the examined su ...19902136428
[campylobacter pylori--relation to gastric pathology and the effect of bismuth therapy].twenty-four patients with symptoms of gastric dyspepsia and active chronic gastritis in the antral mucosa were examined for the presence of campylobacter pylori before and after treatment with bismuth salts (12 patients) and antacids (12 patients). bismuth, contrary to the used antacid, significantly reduced the finding of c. pylori in the antral mucosa and had a favourable effect on the active form of chronic antral gastritis.19902136429
[morphologic changes in chronic active superficial campylobacter pylori-positive antrum gastritis after treatment with bismuth].the authors investigated the effect of one month bismuth treatment (bismuthi citrici 120 mg per capsule), 4 x 120 mg/day, on morphological changes of the gastric mucosa along with the effect on campylobacter pylori (cp) in a group of 23 probands with histologically verified active superficial cp positive antrum gastritis. the probands suffered only from functional dyspepsia, to eliminate the action of other disease of the digestive tract on chronic gastritis. complete eradication of cp occurred ...19902136460
[the isolation by blood culture of campylobacter is no longer a rarity]. 19902138773
[epidemiology of campylobacter jejuni/coli].the authors describe the epidemic incidence of diseases caused by campylobacter jejuni/coli. during the 5-week follow up period (june 1-july 1, 1988) a total of 74 subjects fell sick. in 31 instances the suspect factor of transmission was non-pasteurized cheese prepared from sheep's milk. in this group of patients a significant shift to higher age categories was noted, contrary to the other 43 diseases. it did not prove possible to isolate campylobacter from cheese, smears from the cottage or fr ...19902139812
[competitive activity of a bacterial preparation on colonization and pathogenicity of c. pylori. a clinical study].the authors studied the ability of a live polymicrobial formulation containing lactobacillus acidophilus 10(9) and bifidobacterium bifidum 10(9) to act as an "ecological" therapy for gastritis and duodenitis. the study was carried out in volunteers suffering from these disorders which nowadays are attributed to c. pylori. the results obtained confirm previous findings in experimental animals. the polymicrobial formulation was found to be able to compete effectively with the microorganism respons ...19902147608
[clinical and epidemiologic findings in studies of campylobacter infections].during the period between may 1987 and april 1989 from 929 patients hospitalized at the clinic for infectious diseases with acute diarrhoeal disease campylobacter infection was detected in 10.6%; a bacterial aetiology was confirmed in 29%. the maximum incidence of campylobaterioses was in the summer months, among those affected children aged 1-14 years predominated. analysis of the course of the disease in children under three years revealed typical high fever and blood in the faeces--in half th ...19902150606
in vitro susceptibility of "campylobacter upsaliensis" to twenty-four antimicrobial agents.the in vitro susceptibility of 41 strains of "campylobacter upsaliensis" to 24 antimicrobial agents was determined using a broth microdilution procedure. most isolates were susceptible to the fluoroquinolones and beta-lactam antibiotics tested, but all strains were resistant to trimethoprim (mbcs greater than or equal to 128 micrograms/ml) and teicoplanin (mbcs greater than or equal to 32 micrograms/ml). these agents may be useful in a selective isolation medium for "campylobacter upsaliensis".19902150816
[the suitability of selected humic acid batches as culture media additives for isolating thermophilic campylobacter species].using freshly poured respectively five days under light and air stored campylobacter media blood or lignite humic acids containing mueller-hinton-agar proved to be better for isolation of a campylobacter field strain than unsupplemented or peat humic acids containing media. the observed differences between the used media are probably caused by three different ability to prevent the accumulation of a photochemically induced toxicity by storing of media under light and air. the storage of blood re ...19902150934
campylobacter jejuni-induced vasculitis. 19902151678
[sensitivity of enteropathogenic bacteria to furazolidone].the sensitivity in vitro of 348 strains of 18 enteropathogen agents to furazolidone was investigated during the period of 1987-1989. all strains of shigella sonnei (17), s. flexneri (17), s. boydii (16), escherichia coli enteropathogen (40), e. coli enteroinvasive (20), campylobacter jejuni (50), vibrio cholerae 01 (5), vibrio cholerae non 01 (5), v. parahaemolyticus (5), v. alginolyticus (2), aeromonas hydrophila (5), a. caviae (5), a. sobria (5), and plesiomonas shigelloides (12) were sensitiv ...19902152485
failure of azythromycin to eradicate campylobacter pylori from the stomach because of acquired resistance during treatment. 19902153337
typing of campylobacter pylori by bacterial dna restriction endonuclease analysis and determination of plasmid profile.campylobacter pylori isolates from 37 symptomatic patients and 3 asymptomatic volunteers were examined by chromosomal dna restriction endonuclease analysis and determination of plasmid profile. restriction digests with hindiii, haeiii, pvuii, and bglii produced clear and reproducible results that permitted discrimination between different strains. only 35% of c. pylori isolates were found to have plasmid dna. isolates from different patients, including those from two pairs of siblings, had uniqu ...19902153701
clinical and parasitologic aspects of cryptosporidiosis in nonhuman primates.eighty-one cases of acute cryptosporidiosis were diagnosed among 157 (52%) infant primates, predominantly macaca nemestrina, housed in the nursery unit of the washington regional primate research center. the mean age at onset of oocyst passage was 38 +/- 25 days. the outbreak was confined to the nursery and no cases were detected among juvenile or adult primates housed in other rooms within the colony. all but one animal manifested symptoms of enteric infection, including severe diarrhea and deh ...19902153858
effect of enteroviruses on adherence to and invasion of hep-2 cells by campylobacter isolates.coinfection of hep-2 epithelial cells with coxsackievirus b3, echovirus 7, poliovirus (lsc type 1), porcine enterovirus, and campylobacter isolates was performed to determine if a synergistic effect could be obtained. the invasiveness of campylobacter jejuni atcc 33560 was significantly increased for hep-2 cells preinfected with echovirus 7, coxsackievirus b3, and uv-inactivated (noninfectious) coxsackievirus b3 particles. additionally, the invasiveness of c. jejuni m96, a clinical isolate, was ...19902156779
risk of diarrhea during the first year of life associated with initial and subsequent colonization by specific enteropathogens.the incidence of colonization by enteropathogenic, enterotoxigenic, enteroinvasive, and enterohemorrhagic escherichia coli (detected by dna hybridization with specific radiolabeled probes), salmonella sp., shigella sp., campylobacter jejuni, and rotavirus was related to the presence of diarrhea in a cohort of 75 rural infants followed longitudinally during the first year of life. the study was carried out between august 1985 and february 1987 in the village of lugar sobre la tierra blanca, in th ...19902157338
is herpes simplex virus associated with peptic ulcer disease?to test the hypothesis that herpes simplex virus type 1 (hsv-1) may be associated with peptic ulcer disease, we examined ulcerative lesions of the distal stomach and proximal duodenum for the presence of nucleic acids and antibodies specific for hsv-1. utilizing in situ hybridization, immunocytochemistry, and polymerase chain reaction with sequencing, gastric or duodenal tissues from 4 of 22 patients (18%) with documented peptic ulcer disease demonstrated the presence of both specific hsv-1 nucl ...19902157877
[acute gastroenteritis in childhood. epidemiological comments on a hospital case series].thirty children suffering from acute diarrhoea have been examined over a period of 10 months. salmonellae were present in 16.6% of the cases, campylobacter in 10%, e. coli in 60%, rotavirus in 10%. the monthly incidence of the infections has been studied, verifying values which fall into line with those reported by other authors. it has also been observed that macroscopic blood, fecal leucocytes are present in diarrhoea by bacterial agents, while kerry test is more indicative of infections by ro ...19902158011
[aids and gastrointestinal tract: a summary for gastroenterologists and surgeons].the majority of patients with aids suffer from diarrhea and weight loss, as well as opportunistic infection and tumors of the gastrointestinal tract; endoscopy is frequently necessary. often, but not always, it is possible to identify an opportunistic tumor or infection which explains the patient's signs and symptoms. in other cases, hiv may itself be pathogenic. the most important opportunistic pathogens are candida albicans (stomatitis and esophagitis), cytomegalovirus and herpes simplex virus ...19902159657
identification of campylobacter jejuni surface proteins that bind to eucaryotic cells in vitro.to understand the role of campylobacter jejuni surface proteins in the interaction of c. jejuni with cultured mammalian cell lines in vitro, we developed a ligand-binding assay. this procedure allowed us to antigenically identify c. jejuni outer membrane proteins (omps) that attach to intact host cell membranes. omps isolated from an invasive strain and a less invasive strain were antigenically indistinguishable. however, we found that proteins with molecular masses of 28 and 32 kilodaltons (kda ...19902160431
prospective study of diarrheal diseases in venezuelan children to evaluate the efficacy of rhesus rotavirus vaccine.the efficacy of a rhesus rotavirus vaccine (mmu 18006, serotype 3) against infantile diarrhea was evaluated by active home surveillance of a group of 320 children 1-10 months of age in caracas, venezuela. during a 1 year period following oral administration of vaccine or placebo under a double-masked code, over 600 diarrheal episodes were detected. etiologic studies revealed that heat-stable toxin (st) producing enterotoxigenic e. coli (etec) was the most common diarrheal agent detected (34%) fo ...19902160516
persistent excretion of rotavirus by pregnant cows.in a study of the epizootiology and prevalence of enteropathogens which may be involved in neonatal calf diarrhoea, 10 in-calf cows from a herd with a history of rotavirus-induced calf diarrhoea were monitored over a period of six to seven months. all the cows excreted rotavirus intermittently without showing any clinical signs, and 21.8 per cent of faecal samples contained rotavirus. reoviruses were isolated from 87 per cent of the samples from the cows, and from all the 10 calves born to them. ...19902164274
increased tight junction width in two children with ménétrier's disease.protein-losing enteropathy (ple) and edema are usually the most prominent clinical features in children with ménétrier's disease. however, the changes in gastrointestinal mucosa that can cause ple have not been described yet in children. we studied by electron microscopy the mucosa of the gastric fundus, which is the site where macroscopic changes are most prominent, in two children with ménétrier's disease. we found that tight junction width was increased to 10.5 +/- 0.94 nm (mean +/- 1 sd) in ...19902167358
emergence of aminoglycoside resistance genes aada and aade in the genus campylobacter.resistance to streptomycin or spectinomycin or both in five campylobacter coli strains, two campylobacter jejuni strains, and a campylobacter-like strain was studied by enzymatic assays and dot blot hybridization. resistance was due to 6- or 3",9-aminoglycoside adenylyltransferases and to new types of phospho- and adenylyltransferases.19902168151
use of pulsed-field agarose gel electrophoresis to size genomes of campylobacter species and to construct a sali map of campylobacter jejuni ua580.to determine the physical length of the chromosome of campylobacter jejuni, the genome was subjected to digestion by a series of restriction endonucleases to produce a small number of large restriction fragments. these fragments were then separated by pulsed-field gel electrophoresis with the contour-clamped homogeneous electric field system. the dna of c. jejuni, with its low g+c content, was found to have no restriction sites for enzymes noti and sfii, which cut a high-g+c regions. most of the ...19902168376
catalase activity in campylobacter jejuni: comparison of a wild-type strain with an aerotolerant variant.a comparison of campylobacter jejuni vpi strain h840 (atcc 29428), which can grow at o2 levels up to 15%, with variant strain mc711-01 (which can grow at o2 levels up to 21-26%) indicated that the specific activity of catalase in crude cell extracts was higher in the variant by a factor of 1.6 to 2.5, depending on cultural conditions. smaller differences occurred with superoxide dismutase activity, while peroxidase activities were invariably lower in the variant strain. the variant strain was mu ...19902168804
signature patterns of dna restriction fragments of helicobacter pylori before and after treatment.the genomic dna of helicobacter pylori was studied by electrophoretic analysis of restriction endonuclease fragments. twenty seven isolates from eight patients in the united kingdom, obtained before and after treatment with nitrofurantoin, and two reference strains from australia and peru were investigated. digestion of dna with haeiii, which gave the clearest band pattern of the 20 enzymes tested, showed that each set of isolates from a single patient had a unique band pattern. the dna signatur ...19902169483
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