Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| characterization of a new picorna-like virus, himetobi p virus, in planthoppers. | picorna-like virus particles, 29 nm in diameter, were purified from apparently healthy laodelphax striatellus fallen. the virus particles had a buoyant density of 1.352 g/ml in cscl and a sedimentation coefficient of 161 s. the virus capsid proteins consisted of three major polypeptides of m(r)s 36,500, 33,000 and 28,000, and three minor polypeptides. the virus contained a major ssrna of m(r) 2.8 x 10(6) and was also frequently associated with a minor dsrna of m(r) 4 x 10(6). the 3' end of the s ... | 1992 | 1634869 |
| phases of maize rough dwarf virus multiplication in the vector laodelphax striatellus (fallén). | 1970 | 5422718 | |
| genetic variation in the ability of a planthopper vector; laodelphax striatellus (fallén) to acquire the rice stripe virus. | 1967 | 6025062 | |
| electrophoretic karyotype of intracellular yeast-like symbiotes in rice planthoppers and anobiid beetles. | chromosomal dna molecules of intracellular yeast-like symbiotes (yls) of three species of rice planthoppers and two species of anobiid beetles have been separated by pulsed-field gel electrophoresis. probable chromosome numbers of nilaparvata lugens, sogatella furcifera, and laodelphax striatellus, were 4, 4, and 5, respectively, and tentative genome sizes were 17.3, 17.6, and 20.1 mbp, respectively, based upon migration of individual chromosome-sized dna relative to the size standards of schizo ... | 1995 | 7722339 |
| phylogenetic position of yeast-like symbiotes of rice planthoppers based on partial 18s rdna sequences. | the rice planthoppers, nilaparvata lugens, sogatella furcifera, and laodelphax striatellus, harbor intracellular yeast-like symbiotes (yls) in the fat body. the yls are obligated endosymbiotes and are transovarially inherited. nucleotide sequences of the 18s rrna genes from the yls were analyzed by direct sequencing. the yls of the three species of planthoppers were monophyletic and their taxonomic positions were in the class pyrenomycetes in the subphylum ascomycotina. | 1995 | 7787846 |
| nonpathogenic nilaparvata lugens reovirus is transmitted to the brown planthopper through rice plant. | nilaparvata lugens reovirus (nlrv) was found in a colony of the brown planthopper, n. lugens, an important plant virus vector. unlike with usual phytopathogenic reoviruses, there are no visible symptoms on a rice plant which has been attacked by nlrv-infected planthoppers. manner of transmission, host range, and multiplication of nlrv in rice plant were studied by enzyme-linked immunosorbent assay or polymerase chain reaction using reverse transcriptase. nlrv was transovumly transmitted to about ... | 1995 | 7871743 |
| pcr-based detection of wolbachia, cytoplasmic incompatibility microorganisms, infected in natural populations of laodelphax striatellus (homoptera: delphacidae) in central japan: has the distribution of wolbachia spread recently? | cytoplasmic incompatibility is caused in various insects by intracellular infection with rickettsia-like microorganisms of the genus wolbachia. in japan laodelphax striatellus shows unidirectional cytoplasmic incompatibility between northeastern and southwestern populations. in this study, nine natural populations of l. striatellus collected from central japan, including the geographic boundary between the two cytotype populations, were analysed for wolbachia infection by pcr using primers speci ... | 1995 | 8825761 |
| allozyme polymorphism and geographic variation in the small brown planthopper, laodelphax striatellus (homoptera: delphacidae). | the small brown planthopper, laodelphax striatellus, immigrates annually into japan over the east china sea from the asian mainland. it is not known whether this long-distance dispersal has any effect on the genetic structure of japanese l. striatellus populations. the dispersal of l. striatellus is suspected to be relevant to the population dynamics of infection with the parasitic bacterium wolbachia, which causes cytoplasmic incompatibility in l. striatellus. wolbachia infection has spread wit ... | 1997 | 9559095 |
| detection and assignment of proteins encoded by rice black streaked dwarf fijivirus s7, s8, s9 and s10. | the proteins encoded by rice black streaked dwarf fijivirus (rbsdv) genomic segments 7-10 (s7-s10) were characterized. open reading frames (orfs) from these segments were expressed as fusion proteins in escherichia coli. antibodies raised against the expressed products were used as probes to determine whether the viral orfs encode structural proteins. in western blots, antibodies to the expressed s8 and s10 products reacted with a core capsid (65 kda) and a major outer capsid (56 kda) protein, r ... | 1998 | 9634092 |
| determining the nucleotide sequence and capsid-coding region of himetobi p virus: a member of a novel group of rna viruses that infect insects. | we determined the complete genome sequence of himetobi p virus (hipv), an insect picorna-like virus, which was isolated from the small brown planthopper, laodelphax striatellus. the genome of hipv consists of 9,275 nucleotides excluding the poly (a) tail, and contains two large open reading frames (orfs), which were separated by a 176-nucleotide noncoding region. the deduced amino acid sequence of the first orf contains core motifs of picornaviral helicase, protease, and rna-dependent rna polyme ... | 1999 | 10550677 |
| [phylogenetic relationships and evolution of yeast-like symbionts of the small brown planthoppers based on partial 18s rdna sequences]. | the yeast-like symbionts are isolated and purified from the small brown planthoppers collected from five areas in china. the 18s rdna sequences of them are determined. combined with the sequences of other fungi, we have constructed a comprehensive phylogenetic tree. the results suggested that the taxonomic positions of the yls are in the class pyrenomycetes in the subphylum ascomycotina and the yls have the closest relationship with h. chrysospermus. the yls of different areas in china and japan ... | 2000 | 10887693 |
| wolbachia infection and cytoplasmic incompatibility in the cricket teleogryllus taiwanemma. | wolbachia are cytoplasmically inherited bacteria found in many arthropods. they induce various reproductive alterations in their hosts, including cytoplasmic incompatibility, thelytokous parthenogenesis, feminization and male-killing. in this study, we examined wolbachia infection and its effects on the host cricket teleogryllus taiwanemma. in a phylogenetic study based on the wsp gene coding for a wolbachia surface protein, the wolbachia strain harboured by t. taiwanemma was clustered together ... | 2000 | 10903165 |
| probing stimulants from the rice plant towards the smaller brown planthopper, laodelphax striatellus (fallen) (homoptera: delphacidae). | when adult males of the smaller brown planthopper, laodelphax striatellus were fed on 2% crude rice (leaf and stem) extract containing 15% sucrose there were characteristic stylet sheaths deposited on parafilm membrane. further bioassays with the butanol-soluble fr. of the extract revealed that it is highly effective for the insects. when the butanol fr. was charged on an ods open column and eluted in sequence with 20, 40 and 100% methanol in water, the ods-40% methanol fr. was shown as the most ... | 2015 | 11204184 |
| infection density of wolbachia and incompatibility level in two planthopper species, laodelphax striatellus and sogatella furcifera. | wolbachia, a bacterial endosymbiote of arthropods, causes cytoplasmic incompatibility (ci) in many insect species. ci traits were studied in two planthopper species, laodelphax striatellus and sogatella furcifera, and wolbachia densities in these planthopper species were calculated by quantitative pcr methods. the ci level of l. striatellus was quite high and even aged males strongly caused ci. in contrast, s. furcifera showed partial ci, and males lost their ability to cause ci with age. wolbac ... | 2001 | 11267910 |
| insect symbiosis: derivation of yeast-like endosymbionts within an entomopathogenic filamentous lineage. | yeast-like endosymbionts (ylss) of insects often are restricted to specific hosts and are essential to the host's survival. for example, in planthoppers (homoptera: delphacidae), endosymbionts function in sterol utilization and nitrogen recycling for the hosts. our study, designed to investigate evolutionary changes in the yls lineage involved in the planthopper association, strongly suggests an origin of the ylss from within the filamentous ascomycetes (euascomycetes), not the true yeasts (sacc ... | 2001 | 11371588 |
| wolbachia infection shared among planthoppers (homoptera: delphacidae) and their endoparasite (strepsiptera: elenchidae): a probable case of interspecies transmission. | wolbachia, a group of parasitic bacteria of arthropods, are believed to be horizontally transmitted among arthropod taxa. we present a new probable example of interspecies horizontal transmission of wolbachia by way of an endoparasite based on the conformity of wolbachia gene sequences. field samples of two rice planthoppers, laodelphax striatellus and sogatella furcifera possessed identical wolbachia. among three major endoparasites of planthoppers, a strepsipteran, elenchus japonicus, harboure ... | 2001 | 11555254 |
| [cytopathology of plants infected with viruses. ultrastructure of the leaf cells of cereals affected by rhabdoviruses]. | ultrathin sections of oat, wheat, and ryegrass leaves from healthy plants and plants infected with rhabdoviruses by leafhoppers laodelphax striatellus fallen were studied under the electron microscope. the bacilliform virions often surrounded by endoplasmic reticulum (er) membranes, viroplasm, and tubular structures conforming, in diameter and structure, to the rhabdovirion nucleocapsid were observed in the cytoplasm of leaf cells of the diseased plants. the cereal pseudorosette virus [(165-200) ... | 2002 | 12068435 |
| superinfection of laodelphax striatellus with wolbachia from drosophila simulans. | wolbachia are maternally inherited, intracellular alpha-proteobacteria that infect a wide range of arthropods. they manipulate the reproduction of hosts to facilitate their spread into host populations, through ways such as cytoplasmic incompatibility (ci), parthenogenesis, feminization and male killing. the influence of wolbachia infection on host populations has attracted considerable interest in their possible role in speciation and as a potential agent of biological control. in this study, w ... | 2003 | 12522428 |
| in vitro cultivation of wolbachia in insect and mammalian cell lines. | wolbachia infecting the small brown planthopper, laodelphax striatellus, were successfully maintained and cultivated in two insect and one mammalian cell lines. the bacteria with the planthopper ovary were introduced into the flasks with the cultures of the cell lines. the wolbachia proliferated in mosquito (aedes albopictus) and lepidopteran (heliothis zea) cell lines and in the mouse cell line, l929. proliferation of wolbachia was confirmed by electron microscopy and quantitative polymerase ch ... | 2002 | 12534342 |
| detecting rice stripe virus (rsv) in the small brown planthopper (laodelphax striatellus) with high specificity by rt-pcr. | rice stripe disease, caused by rice stripe virus (rsv), may lead to severe or even crippling losses in many rice-cultured countries and regions. as the most important vector of rsv, the small brown planthopper (sbph) (laodelphax striatellus) is largely responsible for the epidemic phase of the disease. therefore, a rapid identification of rsv in the sbph is of a great need for disease forecasting. a reverse transcription polymerase chain reaction (rt-pcr) assay is described to amplify a rsv gene ... | 2003 | 12951219 |
| [qtl analysis for rice stripe disease resistance gene using recombinant inbred lines (rils) derived from crossing of kinmaze and dv85]. | rice stripe disease transmitted by small brown planthopper (laodelphax striatellus fall.) is one of the most serious viral diseases in east asia. the disease is severely epidemic in most rice growing areas where the main cultivars are susceptible or moderately susceptible to rice stripe virus. in this research, a recombinant inbred lines (rils) population of 81 lines derived from a cross of kinmaze (japonica)/dv85(indica) by the single seed descent method was used to detect quantitative trait lo ... | 2004 | 15195569 |
| cloning and characterization of a beta3 tubulin cdna from the small brown planthopper, laodelphax striatellus. | 2005 | 15859520 | |
| detection and localization of rice stripe virus gene products in vivo. | the genome of the tenuivirus, rice stripe virus (rsv) comprises four rnas, the smallest three of which each contain two open reading frames (orfs) arranged in an ambisense manner. the expression of the orfs from rnas 2-4 in plants and the insect vector, laodelphax striatellus, was studied using antisera raised against the gene products. in western blotting of the proteins from infected plants, the molecular masses of p2, p3, pc3 (nucleocapsid protein, n) and p4 (major non-structural protein, ncp ... | 2005 | 16025247 |
| phylogenetic position of the yeast-like symbiotes of tagosodes orizicolus (homoptera: delphacidae) based on 18s ribosomal dna partial sequences. | tagosodes orizicolus muir (homoptera: delphacidae), the endemic delphacid species of tropical america carries yeast-like symbiotes (yls) in the abdominal fat bodies and the ovarial tissues, like other rice planthoppers of asia. these yls are obligate symbiotes, which are transmitted transovarially, and maintain a mutualistic relationship with the insect host. this characteristic has made in vitro culture and classification of yls rather difficult using conventional methods. nevertheless, microor ... | 2004 | 17361570 |
| transmission of rice stripe virus acquired from frozen infected leaves by the small brown planthopper (laodelphax striatellus fallen). | rice stripe disease, caused by rice stripe virus (rsv), is one of the most serious rice diseases in temperate and subtropical regions of the world. since rsv is not transmissible mechanically, an insect transmission test was the original basis for identification of the viral population and cultivar resistance. a simple, rapid and reliable method is described by which virus-free small brown planthoppers acquired rsv from frozen infected rice leaves and transmitted the virus to healthy rice plants ... | 2007 | 17614147 |
| quantitative trait loci mapping of resistance to laodelphax striatellus (homoptera: delphacidae) in rice using recombinant inbred lines. | laodelphax striatellus fallén (homoptera: delphacidae), is a serious pest in rice, oryza sativa l., production. a mapping population consisting of 81 recombinant inbred lines (rils), derived from a cross between japonica' kinmaze' and indica' dv85' rice, was used to detect quantitative trait loci (qtls) for the resistance to l. striatellus. seedbox screening test (sst), antixenosis test, and antibiosis test were used to evaluate the resistance response of the two parents and 81 rils to l. striat ... | 2007 | 17849901 |
| molecular cloning of a gaba receptor subunit from laodelphax striatella (fallén) and patch clamp analysis of the homo-oligomeric receptors expressed in a drosophila cell line. | a cdna encoding a gamma-aminobutyric acid (gaba) receptor subunit was cloned from the small brown planthopper laodelphax striatella. the l. striatella gaba receptor subunit was found to have high amino acid sequence similarity to the bd-type splice variant of the drosophila gaba receptor rdl subunit and several other gaba receptor subunits, with identities of over 70%. the cdna was inserted into the expression vector pac5.1-lac-hygro. clonal cell lines stably expressing homo-oligomeric l. striat ... | 2007 | 18093001 |
| a one-step real time rt-pcr assay for quantifying rice stripe virus in rice and in the small brown planthopper (laodelphax striatellus fallen). | rice stripe virus (rsv) is an important pathogen affecting rice production in subtropical and temperate regions. one-step real time rt-pcr methods using the taqman probe are described for quantitative detection of rsv in rice tissues and in laodelphax striatellus fallen, the small brown planthopper (sbph). primers and probe for specific detection of rsv were designed within the conserved region identified within the coat protein (cp) gene sequence. a dna fragment was amplified for mimicking the ... | 2008 | 18586332 |
| complete mitochondrial genome of the small brown planthopper, laodelphax striatellus (delphacidae: hemiptera), with a novel gene order. | we determined the first complete mitochondrial genome (mitogenome) sequence from a representative of the insect family delphacidae, laodelphax striatellus. the 16,513 bp long l. striatellus mitogenome encodes 13 putative proteins, two ribosomal rnas, and 22 transfer rnas, and contains a putative control region (or a+t-rich region). the nucleotide composition is biased toward adenine and thymine (77.2% a+t), and the amino acid composition is affected to a similar degree by the at mutational bias. ... | 2009 | 19968473 |
| transinfection of wolbachia in planthoppers: nymphal injection of cultured wolbachia and infection dynamics. | wolbachia species are intracellular symbionts that cause reproductive alterations in arthropods. transinfection experiments have been performed in many arthropod species to elucidate the interaction between wolbachia and a new host. to ease transinfection of this bacterium to new arthropod hosts, we introduced two techniques: nymphal injection instead of embryonic injection and the use of a cultured source of wolbachia instead of direct transfer from donors to recipients. wolbachia in the small ... | 2009 | 20021758 |
| massively parallel pyrosequencing-based transcriptome analyses of small brown planthopper (laodelphax striatellus), a vector insect transmitting rice stripe virus (rsv). | the small brown planthopper (laodelphax striatellus) is an important agricultural pest that not only damages rice plants by sap-sucking, but also acts as a vector that transmits rice stripe virus (rsv), which can cause even more serious yield loss. despite being a model organism for studying entomology, population biology, plant protection, molecular interactions among plants, viruses and insects, only a few genomic sequences are available for this species. to investigate its transcriptome and d ... | 2010 | 20462456 |
| cross-resistance and possible mechanisms of chlorpyrifos resistance in laodelphax striatellus (fallén). | laodelphax striatellus (fallén) is a major pest of cultivated rice and is commonly controlled in china with the organophosphate insecticides. to develop a better resistance management strategy, a chlorpyrifos-resistant strain of l. striatellus was selected in the laboratory, and its cross-resistance to other insecticides and possible mechanisms of the chlorpyrifos resistance were investigated. | 2010 | 20582994 |
| expression of defense genes and activities of antioxidant enzymes in rice resistance to rice stripe virus and small brown planthopper. | the rice variety tai06-1 is resistant to rice stripe disease and xiushui63 is a highly susceptible rice variety to this disease. these two varieties were used to analyze the expression patterns of defense genes and antioxidant defense responses at the seedling stage, upon feeding with viruliferous small brown planthopper (sbph) and nonviruliferous sbph, respectively. the expression levels of cp (coat protein) gene of rice stripe virus (rsv) were higher upon feeding with viruliferous sbph in xius ... | 2011 | 21300551 |
| the a2'n mutation of the rdl gamma-aminobutyric acid receptor conferring fipronil resistance in laodelphax striatellus (hemiptera: delphacidae). | the planthopper laodelphax striatellus (fallén) (hemiptera: delphacidae) is a serious insect pest of rice, oryza sativa l., and has developed resistance to fipronil in japan. sequence analysis of l. striatellus rdl gamma-aminobutyric acid (gaba) receptor subunit (ls-rdl) genes from a fipronil-resistant population and a fipronil-susceptible strain identified the a2'n mutation (index number for m2 membrane-spanning region), that was previously implicated in fipronil resistance in the planthopper s ... | 2011 | 21510217 |
| permanent genetic resources added to molecular ecology resources database 1 january 2009-30 april 2009. | this article documents the addition of 283 microsatellite marker loci to the molecular ecology resources database. loci were developed for the following species: agalinis acuta; ambrosia artemisiifolia; berula erecta; casuarius casuarius; cercospora zeae-maydis; chorthippus parallelus; conyza canadensis; cotesia sesamiae; epinephelus acanthistius; ficedula hypoleuca; grindelia hirsutula; guadua angustifolia; leucadendron rubrum; maritrema novaezealandensis; meretrix meretrix; nilaparvata lugens; ... | 2009 | 21564911 |
| genetic dissection of the resistance to rice stripe virus present in the indica rice cultivar 'ir24' | rice stripe disease, caused by rice stripe virus (rsv) and transmitted by the small brown planthopper (laodelphax striatellus fallen), is one of the most serious viral diseases of rice in temperate east asian production regions. prior quantitative trait loci (qtl) mapping has established that oryza sativa l. subsp. indica 'ir24' carries positive alleles at the three loci qstv3, qstv7, and qstv11-i. here, we report an advanced backcross analysis based on three selected chromosome segment substitu ... | 2011 | 21793697 |
| Identification of yeast associated with the planthopper, Perkinsiella saccharicida: potential applications for Fiji leaf gall control. | Yeasts associate with numerous insects, and they can assist the metabolic processes within their hosts. Two distinct yeasts were identified by PCR within the planthopper Perkinsiella saccharicida, the vector of Fiji disease virus to sugarcane. The utility of both microbes for potential paratransgenic approaches to control Fiji leaf gall (FLG) was assessed. Phylogenetic analysis showed one of the microbes is related to yeast-like symbionts from the planthoppers: Laodelphax striatellus, Nilaparvat ... | 2011 | 21850475 |
| five proteins of laodelphax striatellus are potentially involved in the interactions between rice stripe virus and vector. | rice stripe virus (rsv) is the type member of the genus tenuivirus, which relies on the small brown planthopper (laodelphax striatellus fallén) for its transmission in a persistent, circulative-propagative manner. to be transmitted, virus must cross the midgut and salivary glands epithelial barriers in a transcytosis mechanism where vector receptors interact with virions, and as propagative virus, rsv need utilize host components to complete viral propagation in vector cells. at present, these m ... | 2011 | 22028913 |
| suppression of ns3 and mp is important for the stable inheritance of rnai-mediated rice stripe virus (rsv) resistance obtained by targeting the fully complementary rsv-cp gene. | rice stripe virus (rsv) is a viral disease that seriously impacts rice production in east asia, most notably in korea, china, and japan. highly rsv-resistant transgenic japonica rice plants were generated using a dsrnai construct designed to silence the entire sequence region of the rsv-cp gene. transgenic rice plants were inoculated with a population of viruliferous insects, small brown planthoppers (sbph), and their resistance was evaluated using elisa and an infection rate assay. a correlatio ... | 2011 | 22134721 |
| influence of pymetrozine on feeding behaviors of three rice planthoppers and a rice leafhopper using electrical penetration graphs. | pymetrozine reportedly inhibits feeding of plant sap-sucking insects, such as aphids and brown planthopper (nilaparvata lugens (stål)). by using electrical penetration graph (epg), this study was conducted to investigate any differential effect of pymetrozine on the feeding behaviors of four major rice sap-sucking insect species, 1) n. lugens, 2) white-backed planthopper (sogatella furcifera (horváth)), 3) small brown planthopper (laodelphax striatellus (fallen)), and 4) green rice leafhopper (n ... | 2011 | 22299348 |
| ecdysone receptor controls wing morphogenesis and melanization during rice planthopper metamorphosis. | in this study, we cloned full-length ecr cdnas from the small brown planthopper laodelphgax striatellus, the brown planthopper nilaparvata lugens and the white back planthopper sogatella furciferas. this is the first reporting of ecrs from either l. striatellus or s. furciferas. the deduced amino acid sequences of the ecrs show high levels of similarity to each other. the highest transcriptional level of the ecr gene was detected in the mid-fifth instar nymphs of n. lugens. silencing of ecr expr ... | 2012 | 22326762 |
| analysis of rice stripe virus whole-gene expression in rice and in the small brown planthopper by real-time quantitative pcr. | rice stripe virus (rsv) is a typical member of the tenuivirus genus, which is transmitted mainly by the small brown planthopper (sbph) in a persistent, circulative-propagative manner. at present, expression levels of viral genes in rice and in sbph remain unclear. in this study, we investigated rsv gene expression in rsv-infected rice tissues and in viruliferous sbph using a sensitive and reliable real-time quantitative pcr (q-pcr) method. the results revealed that ns3 gene exhibited the highest ... | 2012 | 22404613 |
| enhanced resistance to four species of clypeorrhynchan pests in neotyphodium uncinatum infected italian ryegrass. | particular alkaloids produced by neotyphodium endophytes show toxicity to invertebrates. italian ryegrass (lolium multiflorum lamarck) cultivars and strains that are symbiotic with neotyphodium endophytes have been recently established in japan. n. uncinatum-infected italian ryegrass lines accumulate n-formylloline, a type of loline alkaloid (1-aminopyrrolizidine) showing neurotoxicity to herbivorous insects. this study investigated the toxicity of n-formylloline and resistance of n. uncinatum-i ... | 2012 | 22420264 |
| identification of quantitative trait loci associated with small brown planthopper (laodelphax striatellus fallén) resistance in rice (oryza sativa l.). | f(2) and bc(1) populations derived from the cross between 02428 / rathu heenati were used to investigate small brown planthopper (sbph) resistance. using the f(2) population, three qtls for antixenosis against sbph were located on chromosomes 2, 5 and 6, and accounted for 30.75% of the phenotypic variance; three qtls for antibiosis against sbph were detected on chromosomes 8, 9 and 12. qsbph5-c explaining 7.21% of phenotypic variance for antibiosis was identified on chromosome 5 using the bc(1) ... | 2012 | 22458437 |
| surface display of rice stripe virus nsvc2 and analysis of its membrane fusion activity. | rice stripe virus (rsv) infects rice and is transmitted in a propagative manner by the small brown planthopper. how rsv enters an insect cell to initiate the infection cycle is poorly understood. sequence analysis revealed that the rsv nsvc2 protein was similar to the membrane glycoproteins of several members in the family bunyaviridae and might induce cell membrane fusion. to conveniently study the membrane fusion activity of nsvc2, we constructed cell surface display vectors for expressing nsv ... | 2012 | 22492001 |
| identification and expression profiles of nine glutathione s-transferase genes from the important rice phloem sap-sucker and virus vector laodelphax striatellus (fallén) (hemiptera: delphacidae). | glutathione s-transferases (gsts) have received considerable attention in insects for their roles in insecticide resistance. laodelphax striatellus (fallén) is a serious rice pest. l. striatellus outbreaks occur frequently throughout eastern asia. a key problem in controlling this pest is its rapid adaptation to numerous insecticides. in this research, nine cdnas encoding gsts in l. striatellus were cloned and characterised. | 2012 | 22522784 |
| transcriptome and comparative gene expression analysis of sogatella furcifera (horváth) in response to southern rice black-streaked dwarf virus. | the white backed planthopper (wbph), sogatella furcifera (horváth), causes great damage to many crops by direct feeding or transmitting plant viruses. southern rice black-streaked dwarf virus (srbsdv), transmitted by wbph, has become a great threat to rice production in east asia. | 2012 | 22558400 |
| chitin synthase 1 gene and its two alternative splicing variants from two sap-sucking insects, nilaparvata lugens and laodelphax striatellus (hemiptera: delphacidae). | chitin synthase (chs) is an enzyme that is required for chitin formation in insect cuticles and other tissues. in this study, chs genes from two destructive rice insect pests, the brown planthopper nilaparvata lugens and the small brown planthopper laodelphax striatellus, were cloned. phylogenetic analysis showed that these genes belonged to class chs1 of the chs gene family. most insects possess two chs genes (chs1 and chs2); however, genome and transcriptome searches showed that n. lugens poss ... | 2012 | 22634163 |
| development and characterization of nine polymorphic microsatellites for the small brown planthopper laodelphax striatellus (hemiptera: delphacidae). | nine microsatellite loci were isolated from the genome of laodelphax striatellus (homoptera: delphacidae) by constructing (tc)(6)(ac)(5) and (ag)(6)(ac)(5) compound ssr-enriched libraries using suppression-pcr procedures. these loci were found to be highly polymorphic, with 13 to 30 alleles per locus in the three populations that we investigated (jiangsu, shandong and zhejiang). the observed and expected heterozygosities ranged from 0.255 to 0.833 and 0.392 to 0.929, respectively. these microsat ... | 2012 | 22653602 |
| restriction of viral dissemination from the midgut determines incompetence of small brown planthopper as a vector of southern rice black-streaked dwarf virus. | southern rice black-streaked dwarf virus (srbsdv), a fijivirus, is transmitted by the white-backed planthopper in a persistent-propagative manner. in this study, we found that another planthopper species, the small brown planthopper (sbph), could acquire srbsdv but not transmit it. to identify the transmission barrier for srbsdv in sbphs, sequential infection by srbsdv in the organs of sbphs was studied with immunofluorescence for viral antigens. srbsdv initially entered the epithelial cells of ... | 2012 | 22683297 |
| morphology, distribution, and abundance of antennal sensilla of male and female macropterous and brachypterous small brown planthopper, laodelphax striatellus (fallén) (hemiptera: delphacidae). | the antennal sensilla of both genders of macropterous and brachypterous adults of the small brown planthopper, laodelphax striatellus (fallén) (hemiptera: delphacidae) were examined using light and scanning electron microscopy. scanning electron microscopy revealed seven types of antennal sensilla in adult l. striatellus which were not evenly distributed on all antennal segments. sensilla chaetica, a sensillum campaniformium and a böhm bristle were found on the scape. sensilla chaetica, sensilla ... | 2012 | 22767352 |
| competitive antagonism of insect gaba receptors by iminopyridazine derivatives of gaba. | a series of 4-(6-imino-3-aryl/heteroarylpyridazin-1-yl)butanoic acids were synthesized and examined for antagonism of gaba receptors from three insect species. when tested against small brown planthopper gaba receptors, the 3,4-methylenedioxyphenyl and the 2-naphthyl analogues showed complete inhibition of gaba-induced fluorescence changes at 100 μm in assays using a membrane potential probe. against common cutworm gaba receptors, these analogues displayed approximately 86% and complete inhibiti ... | 2012 | 22925448 |
| diversity of planthoppers associated with the winter rice agroecosystems in southern yunnan, china. | a field survey of the overwintering planthoppers (hemiptera: delphacidae) associated with the rice agroecosystems in southern yunnan was carried out during january-february in 2010 and 2011. 22 species of planthoppers were collected and identified, with one species representing the subfamily stenocraninae and the other 21 species in delphacinae. nycheuma cognatum (muir), peregrinus maidis (ashmead), and pseudosogata vatrenus (fennah) were new provincial records for yunnan. the pest species, soga ... | 2012 | 22958347 |
| wolbachia from the planthopper laodelphax striatellus establishes a robust, persistent, streptomycin-resistant infection in clonal mosquito cells. | the obligate intracellular bacterium, wolbachia pipientis (rickettsiales: anaplasmataceae), distorts reproduction of its arthropod hosts to facilitate invasion of naïve populations. this property makes wolbachia an attractive "gene drive" agent with potential applications in the control of insect vector populations. genetic manipulation of wolbachia will require in vitro systems for its propagation, genetic modification, amplification, and introduction into target insects. here we show that wolb ... | 2012 | 23271364 |
| resistance monitoring and assessment of resistance risk to pymetrozine in laodelphax striatellus (hemiptera: delphacidae). | laodelphax striatellus (fallén) is a major pest of cultivated rice. pymetrozine, a pyridine azomethine compound, represents a novel insecticide with a selective activity against sucking pests. the resistance of l. striatellus to thiamethoxam, nitenpyram, chlorpyrifos, and pymetrozine in five field populations, collected from jiangsu, anhui, and zhejiang in china, was monitored from 2009 to 2011 in laboratory. all the populations kept susceptible to minor resistance to thiamethoxam and nitenpyram ... | 2012 | 23356079 |
| effect of temperature on the development of laodelphax striatellus (homoptera: delphacidae). | temperature has a significant influence on the development of laodelphax striatellus (fallén), an important rice pest insect in east asia. we set eight constant temperatures from 18 to 32 degrees c in 2 degrees c-increments to check the effect of temperature on the developmental rate of this insect species. the developmental durations of eggs and nymphs were observed daily. to ensure the accuracy of developmental durations, 500 initial samples were taken for the nymphal stage at each temperature ... | 2013 | 23448021 |
| an insect cell line derived from the small brown planthopper supports replication of rice stripe virus, a tenuivirus. | a cell line from the small brown planthopper (sbph; laodelphax striatellus) was established to study replication of rice stripe virus (rsv), a tenuivirus. the sbph cell line, which had been subcultured through 30 passages, formed monolayers of epithelial-like cells. inoculation of cultured vector cells with rsv resulted in a persistent infection. during viral infection in the sbph cell line, the viral non-structural protein ns3 co-localized with the filamentous ribonucleoprotein particles of rsv ... | 2013 | 23468422 |
| detection of quantitative trait loci (qtls) for resistances to small brown planthopper and rice stripe virus in rice using recombinant inbred lines. | small brown planthopper (sbph) and rice stripe virus (rsv) disease transmitted by sbph cause serious damage to rice (oryza sativa l.) in china. in the present study, we screened 312 rice accessions for resistance to sbph. the indica variety, n22, is highly resistant to sbph. one hundred and eighty two recombinant inbred lines (rils) derived from a cross of n22 and the highly susceptible variety, ussr5, were used for quantitative trait locus (qtl) analysis of resistances to sbph and rsv. in a mod ... | 2013 | 23591851 |
| identification and biochemical characterization of laodelphax striatellus neutral ceramidase. | ceramidases are a group of enzymes that catalyse hydrolysis of ceramides to generate fatty acid and sphingosine. in this study, we report the cloning and characterization of the rice small brown planthopper laodelphax striatellus neutral ceramidase (ncdase), lsncer. lsncer was identified by sequencing the transcriptome of l. striatellus and is a protein of 717 amino acids with a predicted molecular weight of 79.3 kda. similarly to other known ncdases, the optimum ph for lsncer is 8.0 and the opt ... | 2013 | 23601004 |
| transcription of orfs on rna2 and rna4 of rice stripe virus terminate at an auccggau sequence that is conserved in the genus tenuivirus. | rice stripe virus, the type member of the genus tenuivirus, has four genomic rnas. rnas 2-4 have an ambisense coding strategy and the noncoding intergenic regions (irs) separating the two orfs are thought to function in termination of transcription. sequencing the 3'-untranslated region of transcripts from rna2 and rna4 in virus-infected oryza sativa (the natural host), nicotiana benthamiana (an experimental host) and laodelphax striatellus (the vector), showed that the sequences of p2 and pc2 t ... | 2013 | 23624227 |
| development and preliminary application of a triplex real-time polymerase chain reaction assay for evaluating predation on three planthoppers in a rice ecosystem. | a multiplex real-time quantitative polymerase chain reaction (pcr) assay was developed to simultaneously detect the dna of three rice planthoppers, that is, sogatella furcifera (horváth) (white-backed planthopper), nilaparvata lugens (stål) (brown planthopper) and laodelphax striatellus (fallén) (small brown planthopper), in the gut of their predators. the sets of primers and allglo probes were targeted to the regions of internal transcribed spacer 2 (its2) genes in nuclear ribosomal dna (rdna). ... | 2013 | 23711378 |
| the oxidizing agent, paraquat, is more toxic to wolbachia than to mosquito host cells. | cultured cells provide an important in vitro system for examining metabolic interactions between the intracellular bacterium, wolbachia pipientis, and its insect hosts. to test whether wolbachia-associated changes in antioxidant activities could provide a tool to select for infected cells, we tested the effects of paraquat (pq) on aedes albopictus mosquito cells. like mammalian cells, mosquito cells tolerate pq over a wide range of concentrations, and for considerable lengths of time, depending ... | 2013 | 23719839 |
| characterization of rice black-streaked dwarf virus- and rice stripe virus-derived sirnas in singly and doubly infected insect vector laodelphax striatellus. | replication of rna viruses in insect cells triggers an antiviral defense that is mediated by rna interference (rnai) which generates viral-derived small interfering rnas (sirnas). however, it is not known whether an antiviral rnai response is also induced in insects by reoviruses, whose double-stranded rna genome replication is thought to occur within core particles. deep sequencing of small rnas showed that when the small brown planthopper (laodelphax striatellus) was infected by rice black-str ... | 2013 | 23776591 |
| an injury equivalency system for establishing a common economic threshold for three species of rice planthoppers (hemiptera: delphacidae) in taiwan. | the economic threshold (et) for multiple pest species that share the same injury type on host plants (feeding guild) has been proposed for decision-making in integrated management framework of many defoliating insect pests. however, only a few consider agricultural pests with sucking mouthparts. this study presents the first injury equivalency system for the feeding guild made up of three rice (oryza sativa l.) planthopper (hemiptera: delphacidae) species--nilaparvata lugens (stål), sogatella fu ... | 2013 | 23786072 |
| the complete mitochondrial genomes of two rice planthoppers, nilaparvata lugens and laodelphax striatellus: conserved genome rearrangement in delphacidae and discovery of new characteristics of atp8 and trna genes. | nilaparvata lugens (the brown planthopper, bph) and laodelphax striatellus (the small brown planthopper, sbph) are two of the most important pests of rice. up to now, there was only one mitochondrial genome of rice planthopper has been sequenced and very few dependable information of mitochondria could be used for research on population genetics, phylogeographics and phylogenetic evolution of these pests. to get more valuable information from the mitochondria, we sequenced the complete mitochond ... | 2013 | 23799924 |
| a functional study of two dsrna binding protein genes in laodelphax striatellus. | one of the key limiting factors for using rna interference technology to develop new pest control practices is the toxicological effectiveness. in order to explore potential methods to improve the efficiency of rnai in insects, we cloned two dsrna binding proteins from laodelphax striatellus and investigated their functions. | 2013 | 23828787 |
| various infection status and molecular evidence for horizontal transmission and recombination of wolbachia and cardinium among rice planthoppers and related species. | wolbachia and cardinium are widely distributed and are considered important for their ability to disturb reproduction and affect other fitness-related traits of their hosts. by using multilocus sequence typing (mlst), rflp (restriction fragment length polymorphism) and 16s ribosomal dna gene sequencing methods, we extensively surveyed wolbachia and cardinium infection status of four predominant rice planthoppers and one kind of leafhopper in different rice fields. the results demonstrated that s ... | 2013 | 23955885 |
| high temperature determines the ups and downs of small brown planthopper laodelphax striatellus population. | small brown planthopper, laodelphax striatellus (fallén) numbers usually drop sharply in the summer and revive quickly in the autumn. however, it is unclear whether and how the high temperature plays a role in this process. the effects of durations of heat exposure (33°c) on life-history traits were examined here. exposure of adults for 1 day during the oviposition stage led to a very low survival of nymphs. the average longevity of l. striatellus exposed for 1-31 days from oviposition was signi ... | 2013 | 23955890 |
| influence of rice black streaked dwarf virus on the ecological fitness of non-vector planthopper nilaparvata lugens (hemiptera: delphacidae). | rice black streak dwarf virus (rbsdv) is transmitted by the small brown planthopper (sbph), laodelphax striatellus (fallen). non-vector rice brown planthopper (bph), nilaparvata lugens (stål), shares the same host rice plants with sbph in paddy fields. the changes in nutritional composition of rice plants infected by rbsdv and the ecological fitness of bph feeding on the infected plants were studied under both artificial climate chamber and field conditions. contents of 16 detected amino acids a ... | 2014 | 23956237 |
| male killing caused by a spiroplasma symbiont in the small brown planthopper, laodelphax striatellus. | spiroplasma-mediated late male killing was found in the small brown planthopper, laodelphax striatellus. female-biased colonies (maternal lines, n = 4) were established from planthoppers collected in taiwan and japan. this sex ratio distortion was maternally inherited (sex ratio of total number of progenies [female:male]: 488:0 in f1, 198:7 in f2, 407:0 in f3; likelihood ratio test of all generations, p < 0.0001) and caused by male death during nymphal stages. the female-biased colonies were dou ... | 2013 | 23975837 |
| knockdown of a putative halloween gene shade reveals its role in ecdysteroidogenesis in the small brown planthopper laodelphax striatellus. | ecdysteroid hormone 20-hydroxyecdysone (20e) plays fundamental roles in insect development and reproduction, whereas the primary role of ecdysone (e) is the precursor for 20e. a cytochrome p450 monooxygenase (cyp), encoded by a halloween gene shade (shd, cyp314a1), catalyzes the conversion of e into 20e in representative insect species in diptera, lepidoptera and orthoptera. we describe here the cloning and characterization of lsshd in a hemipteran insect species, the small brown planthopper lao ... | 2013 | 24055487 |
| bt rice expressing cry2aa does not cause direct detrimental effects on larvae of chrysoperla sinica. | to assess the potential effects of cry2aa-expressing insect-resistant bt rice on chrysoperla sinica larvae, we conducted two tritrophic bioassays using a non-target (laodelphax striatellus) and a target herbivore (chilo suppressalis) as prey. none of the tested life-table parameters of c. sinica did differ when fed with l. striatellus nymphs reared on either bt or control rice plants. similarly, c. sinica larval survival and development were not affected when fed c. suppressalis larvae that were ... | 2013 | 24057602 |
| southern rice black-streaked dwarf virus: a white-backed planthopper-transmitted fijivirus threatening rice production in asia. | southern rice black-streaked dwarf virus (srbsdv), a non-enveloped icosahedral virus with a genome of 10 double-stranded rna segments, is a novel species in the genus fijivirus (family reoviridae) first recognized in 2008. rice plants infected with this virus exhibit symptoms similar to those caused by rice black-streaked dwarf virus. since 2009, the virus has rapidly spread and caused serious rice losses in east and southeast asia. significant progress has been made in recent years in understan ... | 2013 | 24058362 |
| biochemical and molecular characterisation and cross-resistance in field and laboratory chlorpyrifos-resistant strains of laodelphax striatellus (hemiptera: delphacidae) from eastern china. | laboratory selection is often employed in resistance mechanism studies because field-derived populations commonly do not have high enough resistance for such studies. in the present study, a field-collected laodelphax striatellus population from eastern china was laboratory selected for chlorpyrifos resistance and susceptibility, and the developed strains, along with a field population, were studied for cross-resistance and resistance mechanisms at biochemical and molecular levels. | 2014 | 24115461 |
| use of a dietary exposure system for screening of insecticidal compounds for their toxicity to the planthopper laodelphax striatellus. | we developed a dietary exposure assay for screening insecticidal compounds for their toxicity and for assessing the side effects of insecticidal proteins produced by genetically engineered (ge) plants on the planthopper laodelphax striatellus fallén. the fitness bioassay confirmed that the diet fulfills the requirements to be used in the dietary exposure system. to validate the efficacy of the dietary exposure system, nymphs of l. striatellus were fed diets treated with different concentrations ... | 2014 | 24115524 |
| the complete mitochondrial genome sequence of sogatella furcifera (horváth) and a comparative mitogenomic analysis of three predominant rice planthoppers. | the white-backed planthopper (wbph), sogatella furcifera (horváth), is one of the most destructive pests of rice crops in many asian countries. using long-pcr and shotgun library methods, we sequenced the entire mitochondrial genomes (mt-genomes) of two wbph individuals. total lengths of the mt-genome of the two wbph individuals were 16,612 bp and 16,654 bp with an identical at content of 76.19%. among the 13 protein coding genes (pcgs), only nad5 used an atypical initiation codon gtg. most of t ... | 2014 | 24120898 |
| single and fused transgenic bacillus thuringiensis rice alter the species-specific responses of non-target planthoppers to elevated carbon dioxide and temperature. | the approval of transgenic bacillus thuringiensis (bt) rice by china was momentous for biotech crops, although it has yet to be approved for commercial production. non-target pest problems in rice paddies, such as the three ecologically similar species of planthoppers nilaparvata lugens, laodelphax striatellus and sogatella furcifera, could become increasingly serious under global climate change. fused (cry1ab/cry1ac) and single (cry1ab) transgenic bt rice were evaluated for effects on species-s ... | 2014 | 24136625 |
| investigation on subcellular localization of rice stripe virus in its vector small brown planthopper by electron microscopy. | rice stripe virus (rsv), which is transmitted by small brown planthopper (laodelphax striatellus fallén, sbph), has been reported to be epidemic and cause severe rice stripe disease in rice fields in many east asian countries, including china. investigation on viral localization in the vector is very important for elucidating transmission mechanisms of rsv by sbph. in this study, transmission electron microscopy and immuno-gold labeling technique were used to investigate the subcellular localiza ... | 2013 | 24139455 |
| dynamics of neotyphodium uncinatum and n-formylloline in italian ryegrass, and their relation to insect resistance in the field. | a fungal endophyte, neotyphodium uncinatum, accumulates n-formylloline, which is toxic to hemipteran insects, in italian ryegrass. this study aimed to clarify the dynamics of n. uncinatum and n-formylloline in italian ryegrass, and their relationship to insect resistance. | 2014 | 24219582 |
| identification of himetobi p virus in the small brown planthopper by deep sequencing and assembly of virus-derived small interfering rnas. | profiling and assembly of virus-derived small interfering rnas (sirnas) using next-generation sequencing technologies have been very useful for identification and diagnosis of a number of plant and invertebrate viruses. in this work, we have conducted high-throughput pyrosequencing and bioinformatic analysis of the small brown planthopper (sbph, laodelphax striatellus), and these analyses unexpectedly showed that the himetobi p virus (hipv) was present in our laboratory cultures. hipv was also f ... | 2014 | 24239755 |
| overexpression of rice black-streaked dwarf virus p7-1 in arabidopsis results in male sterility due to non-dehiscent anthers. | rice black-streaked dwarf virus (rbsdv), a member of the genus fijivirus in the family reoviridae, is propagatively transmitted by the small brown planthopper (laodelphax striatellus fallén). rbsdv causes rice black-streaked dwarf and maize rough dwarf diseases, which lead to severe yield losses in crops in china. although several rbsdv proteins have been studied in detail, the functions of the nonstructural protein p7-1 are still largely unknown. to investigate the role of the p7-1 protein in v ... | 2013 | 24260239 |
| rna interference-mediated silencing of a halloween gene spookier affects nymph performance in the small brown planthopper laodelphax striatellus. | post-embryonic development of insects is highly dependent on ecdysteroid hormone 20-hydroxyecdysone. halloween gene spookier (spok, cyp307a2) has been documented to be involved in ecdysteroidogenesis in drosophila melanogaster and bombyx mori. we describe here the cloning and characterization of halloween gene spookier (lsspok, lscyp307a2) in the small brown planthopper laodelphax striatellus, a hemipteran insect species. lsspok has three insect-conserved p450 motifs, that is, helix-k, perf moti ... | 2015 | 24282064 |
| migration of rice planthoppers and their vectored re-emerging and novel rice viruses in east asia. | this review examines recent studies of the migration of three rice planthoppers, laodelphax striatellus, sogatella furcifera, and nilaparvata lugens, in east asia. laodelphax striatellus has recently broken out in jiangsu province, eastern china. the population density in the province started to increase in the early 2000s and peaked in 2004. in 2005, rice stripe virus (rsv) viruliferous rate of l. striatellus peaked at 31.3%. since then, rice stripe disease spread severely across the whole prov ... | 2013 | 24312081 |
| unusual characteristics of dicistrovirus-derived small rnas in the small brown planthopper, laodelphax striatellus. | in this study, sequences of small rna (srna) libraries derived from the insect vector laodelphax striatellus were assembled into contigs and used as queries for database searches. a large number of contigs were highly homologous to the genome sequence of an insect dicistrovirus, himetobi p virus (hipv). interestingly, hipv-derived srnas had a wide size distribution, and were relatively abundant throughout the 18-30 nt size range with only a slight peak at 22 nt. hipv srnas had a strong bias towa ... | 2014 | 24323637 |
| overexpression of multiple detoxification genes in deltamethrin resistant laodelphax striatellus (hemiptera: delphacidae) in china. | the small brown planthopper (sbph), laodelphax striatellus (fallén), is one of the major rice pests in asia and has developed resistance to multiple classes of insecticides. understanding resistance mechanisms is essential to the management of this pest. biochemical and molecular assays were performed in this study to systematically characterize deltamethrin resistance mechanisms with laboratory-selected resistant and susceptible strains of sbph. | 2013 | 24324548 |
| feeding-induced interactions between nilaparvata lugens and laodelphax striatellus (hemiptera: delphacidae): effects on feeding behavior and honeydew excretion. | using electrical penetration graph, salivary flange, and honeydew measurement, this study investigated the effects of feeding-induced intra- and interspecific interactions on feeding behavior and honeydew excretion of brown planthopper (nilaparvata lugens) compared with small brown planthopper (laodelphax striatellus). results showed that many measures of feeding behavior were affected by feeding-induced intra- and interspecific interactions on two different rice varieties. there were significan ... | 2013 | 24331608 |
| comparative transcriptome analysis of two rice varieties in response to rice stripe virus and small brown planthoppers during early interaction. | rice stripe, a virus disease, transmitted by a small brown planthopper (sbph), has greatly reduced production of japonica rice in east asia, especially in china. although we have made great progress in mapping resistance genes, little is known about the mechanism of resistance. by de novo transcriptome assembling, we gained sufficient transcript data to analyze changes in gene expression of early interaction in response to sbph and rsv infection in rice. respectively 648 and 937 degs were detect ... | 2013 | 24358146 |
| rna interference depletion of the halloween gene disembodied implies its potential application for management of planthopper sogatella furcifera and laodelphax striatellus. | sogatella furcifera and laodelphax striatellus are economically important rice pests in china by acting as vectors of several rice viruses, sucking the phloem sap and blocking the phloem vessels. ecdysteroid hormone 20-hydroxyecdysone regulates insect development and reproduction. a cytochrome p450 monooxygenase cyp302a1 (22-hydroxylase), encoded by the halloween gene disembodied (dib), plays a critical role in ecdysteroidogenesis. the objective of this study is to test whether dib genes are pot ... | 2014 | 24489765 |
| small brown planthopper resistance loci in wild rice (oryza officinalis). | host-plant resistance is the most practical and economical approach to control the rice planthoppers. however, up to date, few rice germplasm accessions that are resistant to the all three kinds of planthoppers (1) brown planthopper (bph; nilaparvata lugens stål), (2) the small brown planthopper (sbph; laodelphax striatellus fallen), and (3) the whitebacked planthopper (wbph, sogatella furcifera horvath) have been identified; consequently, the genetic basis for host-plant broad spectrum resistan ... | 2014 | 24504629 |
| nonstructural protein ns4 of rice stripe virus plays a critical role in viral spread in the body of vector insects. | rice stripe virus (rsv), a tenuivirus, is transmitted by small brown planthopper (sbph) in a persistent-propagative manner. in this study, sequential infection of rsv in the internal organs of sbph after ingestion of virus indicated that rsv initially infected the midgut epithelium, and then progressed to the visceral muscle tissues, through which rsv spread to the entire alimentary canal. finally, rsv spread into the salivary glands and reproductive system. during viral infection, the nonstruct ... | 2014 | 24523924 |
| differential mechanisms of action of the novel γ-aminobutyric acid receptor antagonist ectoparasiticides fluralaner (a1443) and fipronil. | fluralaner (a1443) is an isoxazoline ectoparasiticide that is a novel antagonist of γ-aminobutyric acid (gaba) receptors (gabars), with a potency comparable to that of fipronil, a phenylpyrazole ectoparasiticide. to clarify the biological effectiveness of fluralaner against fipronil-resistant pests, differences in the actions of fluralaner and fipronil on gabars that possess resistance to dieldrin (rdl)-type mutations were evaluated. | 2015 | 24591229 |
| transovarial transmission of a plant virus is mediated by vitellogenin of its insect vector. | most plant viruses are transmitted by hemipteroid insects. some viruses can be transmitted from female parent to offspring usually through eggs, but the mechanism of this transovarial transmission remains unclear. rice stripe virus (rsv), a tenuivirus, transmitted mainly by the small brown planthopper (laodelphax striatellus), is also spread to the offspring through the eggs. here, we used the rsv-planthopper system as a model to investigate the mechanism of transovarial transmission and demonst ... | 2014 | 24603905 |
| a halloween gene shadow is a potential target for rna-interference-based pest management in the small brown planthopper laodelphax striatellus. | laodelphax striatellus is an economically important rice pest in china. ecdysteroid hormone 20-hydroxyecdysone regulates insect development and reproduction. the cytochrome p450 monooxygenase shadow (sad) plays a critical role in ecdysteroidogenesis. here, tests were conducted to establish whether lssad was a potential target gene for rna-interference-based management of l. striatellus. | 2015 | 24648012 |
| depletion of host cell riboflavin reduces wolbachia levels in cultured mosquito cells. | wolbachia is an obligate intracellular alphaproteobacterium that occurs in arthropod and nematode hosts. wolbachia presumably provides a fitness benefit to its hosts, but the basis for its retention and spread in host populations remains unclear. wolbachia genomes retain biosynthetic pathways for some vitamins, and the possibility that these vitamins benefit host cells provides a potential means of selecting for wolbachia-infected cell lines. to explore whether riboflavin produced by wolbachia i ... | 2014 | 24789726 |
| molecular cloning and characterization of the putative halloween gene phantom from the small brown planthopper laodelphax striatellus. | ecdysteroid hormone 20-hydroxyecdysone plays fundamental roles in insect postembryonic development and reproduction. several cytochrome p450 mono-oxygenases (cyps), encoded by the halloween genes, have been documented to be involved in ecdysteroidogenesis in representative insects in diptera, lepidoptera and orthoptera. here the putative halloween gene phantom (phm, cyp306a1) from a hemipteran insect species, the small brown planthopper laodelphax striatellus, was cloned. lsphm shows five insect ... | 2015 | 24954278 |
| bio-effects of near-zero magnetic fields on the growth, development and reproduction of small brown planthopper, laodelphax striatellus and brown planthopper, nilaparvata lugens. | magnetic fields markedly affect the growth and development of many species of organisms potentially due to cryptochrome and endogenous presence of magnetic materials. sensitivity to magnetic fields can also be involved in geomagnetic orientation by some long-distance migratory insects. in this study, near-zero magnetic fields (nzmf) in relation to normal geomagnetic fields (gmf) were setup using the hypomagnetic field space system (hmfs) to investigate the effects of magnetic fields on the growt ... | 2014 | 24995837 |
| identification and characterization of micrornas in small brown planthopper (laodephax striatellus) by next-generation sequencing. | micrornas (mirnas) are endogenous non-coding small rnas that regulate gene expression at the post-transcriptional level and are thought to play critical roles in many metabolic activities in eukaryotes. the small brown planthopper (laodephax striatellus fallén), one of the most destructive agricultural pests, causes great damage to crops including rice, wheat, and maize. however, information about the genome of l. striatellus is limited. in this study, a small rna library was constructed from a ... | 2014 | 25057821 |
| a novel, in vivo, indoor method to preserve rice black-streaked dwarf virus in small brown planthopper using wheat seedling as a bridge host. | rice black-streaked dwarf virus (rbsdv) naturally infects gramineae plants through small brown planthopper (sbph) as a vector. however, rbsdv cannot be transmitted to the sbph offspring through transovarian transmission. wheat plant, an important intermediate host in winter, is essential for the completion of the annual cycle of rbsdv in farm ecosystem. we developed a novel, in vivo, indoor method to preserve rbsdv in sbph using wheat seedling as a bridge host. the temperature range of 23-27°c w ... | 2014 | 25075933 |
| proteomic profiling of a robust wolbachia infection in an aedes albopictus mosquito cell line. | wolbachia pipientis, a widespread vertically transmitted intracellular bacterium, provides a tool for insect control through manipulation of host-microbe interactions. we report proteomic characterization of wstr, a wolbachia strain associated with a strong cytoplasmic incompatibility phenotype in its native host, laodelphax striatellus. in the aedes albopictus c/wstr1 mosquito cell line, wstr maintains a robust, persistent infection. ms/ms analyses of gel bands revealed a protein 'footprint' do ... | 2014 | 25155417 |
| diapause induction and termination in the small brown planthopper, laodelphax striatellus (hemiptera: delphacidae). | the small brown planthopper, laodelphax striatellus (fallén) enters the photoperiodic induction of diapause as 3rd or 4th instar nymphs. the photoperiodic response curves in this planthopper showed a typical long-day response type with a critical daylength of approximately 11 h at 25 °c, 12 h at 22 and 20 °c and 12.5 h at 18 °c, and diapause induction was almost abrogated at 28 °c. the third stage was the most sensitive stage to photoperiod. the photoperiodic response curve at 20 °c showed a gra ... | 2014 | 25188306 |