Publications
Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
---|
[cardenolides in callus cultures of digitalis purpurea and digitalis lanata. iii. callus cultures derived from roots (author's transl)]. | 1979 | 432306 | |
[biotransformation of cardenolides by cell suspension cultures of digitalis lanata and thevetia neriifolia (author's transl)]. | 1977 | 840920 | |
[stability and technology of ampule solutions of cardenolides of the wooly foxglove (digitalis lanata ehrh.)]. | 2014 | 862945 | |
[observations on the occurrence and the formation of cardenolides in tissue cultures of digitalis purpurea and digitalis lanata. 2. cardenolides in submerse cultures of the leaves of digitalis purpurea (author's transl)]. | 1977 | 866478 | |
[flavonoids in the leaves of digitalis lanata (ehrhart) 1. communication) (author's transl)]. | 1977 | 905411 | |
[incidence and biosynthesis of cardenolides in tissue cultures of digitalis purpurea and digitalis lanata. 1. cardenolides in surface-cultures of cotyledones and leaves of digitalis purpurea]. | 1976 | 948530 | |
[a new method for the quantitative determination of some important glycosides in drug extracts of digitalis lanata (author's transl)]. | 1976 | 959383 | |
application of high-performance liquid chromatography to the separation of cardenolides and the assay of digoxin in digitalis lanata leaf. | 1976 | 984421 | |
fate of c-24 hydrogen atoms of cholesterol during its conversion into tigogenin in digitalis lanata. | 1976 | 988047 | |
[variability in the lanatoside c content of harvested leaves and drugs of digitalis lanata ehrh]. | 1976 | 1023250 | |
two-stage cultivation of digitalis lanata cells: semicontinuous production of deacetyllanatoside c in 20-litre airlift bioreactors. | a two-stage cultivation method was employed to develop a semicontinuous biotransformation process for the production of deacetyllanatoside c, a cardenolide of the important digoxin series. digitoxin was used as the substrate for biotransformation. the process was optimized in 1-l shake flasks and then established on the 20-l scale using two airlift bioreactors, one for cell growth (working volume 12 litres) and another for deacetyllanatoside c production (working volume 18 litres). growth and pr ... | 1990 | 1366814 |
12 beta-hydroxylation of digitoxin by suspension-cultured digitalis lanata cells: production of digoxin in 20-litre and 300-litre air-lift bioreactors. | a biotransformation process for the production of digoxin was developed using digitalis lanata cell suspension cultures. digitoxin was used as the substrate for biotransformation. digoxin production was carried out in a variety of vessels, including 1-l exsiccators, 20-l glass reactors and a 300-l air-lift bioreactor. a culture volume of 200 l was established after 28 d and the cells were then cultured semi-continuously in a 300-l bioreactor employing the draw-fill cultivation method. maximal di ... | 1992 | 1369154 |
[cardenolide glycosides in digitalis crossbreeds. reciprocal crossbreeds of digitalis lanata with other digitalis species]. | 1992 | 1642514 | |
high-performance liquid chromatographic determination of alpha-acetyldigoxin in digitalis lanata leaves. | an analytical method for the determination of alpha-acetyldigoxin in digitalis lanata leaves by hplc has been developed. the procedure consists of extraction of dry leaf powder with 50% methanol and cleanup by a sep-pak c18 cartridge prior to hplc analysis. the quantitation is carried out by the incorporation of beta-methyldigoxin as an internal standard. hplc is performed on an octylsilyl bonded silica column with acetonitrile/methanol/water (100/11/188, v/v). the effluent is monitored by uv ab ... | 1991 | 1776696 |
determination of cardenolides in hairy root cultures of digitalis lanata by enzyme-linked immunosorbent assay. | a competitive elisa technique using digoxin-specific antibody has been developed to determine digoxin and its related compounds in hairy root cultures of digitalis lanata. the elisa could detect 0.2-2 nm digoxin and closely related cardenolides. hairy roots cultured in the dark accumulated very small amounts of cardenolides (0.02-0.07 micrograms/g dry wt), while the content of cardenolides in green hairy roots cultured in the light was increased maximally 600-fold (16.5 micrograms/g dry wt) comp ... | 2013 | 2089119 |
characterization and localization of progesterone 5 alpha-reductase from cell cultures of foxglove (digitalis lanata ehrh). | progesterone 5 alpha-reductase, which catalyses the reduction of progesterone to 5 alpha-pregnane-3,20-dione, was isolated and characterized from cell cultures of digitalis lanata (foxglove). optimum enzyme activity was observed at ph 7.0, and the enzyme had an apparent km value of 30 microm for its substrate progesterone. the enzyme needs nadph as reductant, which could not be replaced by nadh. for nadph, the apparent km value is 130 microm. the optimum temperature was 40 degrees c; at temperat ... | 1990 | 2106876 |
3 beta-hydroxysteroid oxidoreductase in suspension cultures of digitalis lanata ehrh. | a 3 beta-hydroxysteroid oxidoreductase was isolated and characterized in the microsomes of digitalis lanata cell cultures. the enzyme catalyzes the conversion of 5 alpha-pregnane-3,20-dione to 5 alpha-pregnan-3 beta-ol-20-one and requires nad(p)h2. the enzyme was found to have a ph optimum of 8.0. the reaction had an optimum incubation temperature of 25 degrees c with linear reduction for the first 4 h, reaching maximum enzyme activity after 7 h. substrate kinetics for 5 alpha-pregnane-3,20-dion ... | 2009 | 2291772 |
separation and determination of lanatosides in digitalis lanata leaves by high-performance liquid chromatography. | 1990 | 2380317 | |
structural studies on the biosides of digitalis lanata: bisdigitoxosides of digitoxigenin, gitoxigenin and digoxigenin. | the crystal structures and conformations of bisdigitoxosides of digitoxigenin (i), gitoxigenin (ii) and digoxigenin (iii and iv) have been determined using single-crystal x-ray crystallographic techniques. crystals of (i), (ii) and (iv) were grown from ethyl acetate solutions of the glycosides while (iii) was grown from a solution of the digitoxoside in ethanol. as in other cardiac glycosides the ring junctions a-b and c-d are cis. the d ring in these structures shows different conformations whi ... | 1989 | 2619961 |
clinical utility of plasma digoxin measurements. | the results of plasma digoxin concentration (pdc) measurements in 260 patients treated with digitalis lanata preparations (digoxin, lanatoside c, betamethyl-digoxin) allowed the following observations: 1. owing to an important overlap between "toxic" and "nontoxic" pdc values, the method has a limited value in the diagnosis of digitalis toxicity. however, in patients with toxicity associated with pdc under 2 ng/ml, it allows identification digitalis sensitivity. 2. a dosage selection based on pd ... | 2013 | 2749155 |
reconstitution of cytochrome p-450-dependent digitoxin 12 beta-hydroxylase from cell cultures of foxglove (digitalis lanata ehrh.). | cytochrome p-450-dependent digitoxin 12 beta-hydroxylase from cell cultures of foxglove (digitalis lanata) was solubilized from microsomal membranes with chaps (3-[(3-cholamidopropyl)dimethylammonio]propane-1-sulphonic acid). cytochrome p-450 was separated from nadph: cytochrome c (p-450) reductase by ion-exchange chromatography on deae-sephacel. nadph:cytochrome c (p-450) reductase was further purified by affinity chromatography on 2',5'-adp-sepharose 4b. this procedure resulted in a 248-fold p ... | 1988 | 3137929 |
growth and digoxin content in digitalis lanata in controlled conditions and natural environment. | 1988 | 3174863 | |
major cardenolide glycosides in embryogenic suspension cultures of digitalis lanata. | 1987 | 3628562 | |
isolation and quantitative determination of some cardioactive glycosides from digitalis lanata by high-performance liquid chromatography. | a rapid extraction method followed by high-performance liquid chromatographic assay was developed for the quantitative determination of the cardioactive glycosides of digitalis lanata. the leaf samples were extracted with water or aqueous alcohols. the simple extraction method gives a better yield than the methods described previously. lanatoside c and its metabolites have been separated on a reversed-phase column with various mixtures of acetonitrile, methanol, and water as mobile phases for is ... | 1986 | 3740407 |
[cultivation and improvement of digitalis lanata in the netherlands]. | 1985 | 3990875 | |
cultivation and breeding of digitalis lanata in the netherlands. | after the second world war marshall aid funds were used to establish a cooperative organisation for growing, drying, and selling digitalis lanata (and other medicinal, aromatic, and culinary herbs) in the netherlands. the crop is sown in mid april and the fully mechanised harvest of the leaves takes place from september to late november. the leaves are dried for 10-12 hours at 50 degrees c maximum. the aim of breeding trials is to improve leaf production, erect leaf attitude, resistance to septo ... | 1985 | 4041298 |
[effect of microelements on the accumulation of cardiac glycosides in digitalis lanata]. | 1974 | 4463037 | |
[cardiac glycosides. xiv. movement of the lanatoside a level in isolated leaves of digitalis lanata ehrh]. | 1973 | 4694123 | |
reciprocal cross differences in digitalis lanata x d. lutea. | 1973 | 4705800 | |
[biosynthesis of glycosides from digitalis lanata ehrh]. | 1974 | 4845466 | |
effect of the use of different methods of drying of digitalis lanata leaves on their quality and glycosidal content. | 1974 | 4851704 | |
[in vitro demonstration of vegetative and floral organogenetic potentialities in 3 digitalis species: digitalis lanata ehrh., digitalis purpurea l. and its gloxiniaeflora variety]. | 1969 | 4977771 | |
[cardiac glycosides. xii. digoxin, the fermented drug of undulating foxglove (digitalis lanata ehrh)]. | 1972 | 5046008 | |
acetyldigoxin and acetyldigitoxin from digitalis lanata. | 1972 | 5050388 | |
[steroid saponins with more than one sugar chain. vi. lanatigoside and lanagitoside, two bisdesmosidic 22-hydroxyfurostanol glycosides from the leaves of digitalis lanata ehrh]. | 2016 | 5077133 | |
[the influence of air on fermentative reactions in digitalis lanata erh. 2]. | 1972 | 5082269 | |
[spectrophotometric method for a quantitative determination of lanatosides a, b, c in the leaves of digitalis lanata]. | 2014 | 5093349 | |
contribution to the study of storage of digitalis lanata leaves. | 1971 | 5125492 | |
[studies on the glycoside content of digitalis lanata erh. in relation to conservation methods]. | 1971 | 5141261 | |
[changes in the glycoside content in dry leaves of digitalis lanata]. | 1971 | 5154602 | |
the effect of irrigation and nitrogenous fertilizers on the growth and glycosidal content of digitalis lanata. | 1971 | 5154604 | |
biosynthesis of stigmasterol from sitosterol in digitalis lanata. | 1969 | 5344271 | |
conversion of sitosterol to progesterone by digitalis lanata. | 1969 | 5362721 | |
[developing systems for thin-layer chromatography of tinctures from digitalis lanata, convallaria majalis and adonis vernalis]. | 1969 | 5408585 | |
[determination of the content of lanatosides in dry and fresh leaves of digitalis lanata ehrh]. | 1970 | 5428331 | |
a study of the yield and glycosidal content of the leaves of digitalis lanata at different stages of plant growth. | 1970 | 5428504 | |
[cardiotonic glycosides. xi. study on the drying conditions of grecian foxglove leaves (digitalis lanata ehrh)]. | 1970 | 5492518 | |
[effect of moisture and warmth on the content of primary and secondary glycosides in dried leaves of digitalis lanata ehrh]. | 1971 | 5546598 | |
conversion of pregnenolone to digifologenin by digitalis lanata. | 1967 | 5591315 | |
[biological and chemical evaluations of extracts of digitalis lanata e. and digitalis purpurea l]. | 1967 | 5613031 | |
[could the digitalis lanata drug standard be replaced by a pure glycoside from digitalis lanata?]. | 1967 | 5632863 | |
[comparison of the effect of digitalis lanata standard drug with digoxin and cymarin on guinea pigs]. | 1967 | 5632864 | |
the role of deoxycorticosterone in the biosynthesis of cardenolides in digitalis lanata. | the role of deoxycorticosterone in the biosynthesis of digitoxigenin was investigated by the simultaneous administration of deoxy[1,2-(3)h(2)]corticosterone and [4-(14)c]progesterone to a digitalis lanata plant. the biosynthetically formed [(3)h,(14)c]digitoxigenin and deoxy[(3)h,(14)c]corticosterone were isolated and the distribution of the two isotopes in these products was determined. the transformation of progesterone into deoxycorticosterone in vivo was established. the biosynthetic route f ... | 1968 | 5667260 |
investigating digitalis lanata for anabolic effect. | 1968 | 5684381 | |
experimental data about anabolic effect of digitalis lanata. | 1968 | 5687106 | |
"synthesis" of lanatosides in digitalis lanata leaves during the wilting period. | 1968 | 5714601 | |
[biochemical study of digitalis (digitalis lanata ehrb.)]. | 2014 | 5721787 | |
[cardiotonic glycosides. ix. establishment of relation in plant, in harvested leaves and drugs of digitalis lanata ehrh]. | 1969 | 5780146 | |
[content and interrelation of lanatosides in digitalis lanata ehrh. ssp. lanata during the development]. | 1965 | 5853279 | |
[glucodigifucoside, neo-glucodigifucoside and digitoxigenin-allomethyloside from the leaves of digitalis lanata ehrh]. | 1965 | 5868485 | |
[the biosynthesis of the cardenolid- and spirostan glycoside in digitalis lanata ehrh. comparison of formation rates. glycoside and aglycone, 274]. | 1966 | 5924990 | |
[biosynthesis of digitoxose in digitalis lanata ehrh. (preliminary experiment). deoxysugar. 39]. | 1966 | 5924991 | |
[the additive behavior of digitalis lanata glycosides and their significance for the ambulant treatment of cardiac insufficiency]. | 1966 | 5927492 | |
progesterone: its possible role in the biosynthesis of cardenolides in digitalis lanata. | the incorporation of progestrone-7alpha-(3)h and pregnenolone-7alpha-(3)h into digitoxigenin, gitoxigenin, and digoxigenin in isolated, surviving leaves of digitalis lanata was demonstrated. in addition, the conversion of pregnenolone to progesterone in the same system was proved. the results tend to indicate that progesterone is as good a precursor of cardenolides as pregnenolone it is suggested that the biosynthesis of cardenolides might proceed through the intermediacy of progesterone. | 1967 | 6021677 |
[pharmacological properties of adicin, soviet alpha-acetyldigitoxin]. | adicin, soviet alpha-acetyldigitoxin, obtained from digitalis lanata ehrh., c. scrophulariaceae after isolation from it of celanid is a highly effective cardiotonic of the digitalis type of action. it exerts a favourable ino- and tonotropic and an adverse chronotropic action on the heart. this action is brought up in varied animal species and can be observed for a long time (over 3 hours). adicin produces no adverse effect on the coronary blood flow and it is conducive to the improvement of veno ... | 1980 | 7002595 |
quantitative analysis of natural drugs ii. quantitative tlc determination of digoxin and other glycosides in pretreated digitalis lanata extracts. | 1982 | 7090839 | |
3 alpha-hydroxysteroid-5 beta-oxidoreductase in tissue cultures of digitalis lanata. | putative intermediates of cardenolide biosynthesis, namely progesterone, pregnenolone, 5 beta-pregnane-3,20-dione or 5 beta-pregnan-3 beta-ol-20-one, were administered to light- or dark-grown shoot cultures of digitalis lanata. the unsaturated compounds were reduced to their respective 5 alpha-pregnanes, 5 beta-pregnane-3,20-dione was reduced to 5 beta-pregnan-3 alpha-ol-20-one and 5 beta-pregnan-3 beta-ol-20-one was isomerized to the respective 3 alpha-pregnane. suspension cultures of digitalis ... | 2013 | 8251035 |
[determination of cardenolides in digitalis lanata by a solid-phase immunoenzyme method]. | a competitive solid-phase eia technique using high-affinity digoxin-specific monoclonal antibodies has been developed to determine cardenolides in digitalis lanata. the test-system can detect digoxin and closely related compounds in concentration of 0.1 nmol/ml. the content of cardenolides, tested by elisa, correlated well with the total content of digoxin, deslanoside c and lanatoside c as analysed by hplc. the test-system is useful in estimating the productivity of large series and samples of ... | 1993 | 8274170 |
cardiac glycosides in partly submerged shoots of digitalis lanata. | shoot cultures were established from axillary buds (11 strains) or seeds (1 strain) of individual digitalis lanata ehrh. plants and propagated partially submerged in liquid medium. five of these shoot culture strains were characterized with regard to their growth and cardenolide content. the cultures were observed for more than one year and found to be relatively stable with regard to their growth and cardenolide spectrum and yield. the strains examined differed in terms of their total cardenoli ... | 1993 | 8302954 |
high-performance liquid chromatographic determination of cardenolides in digitalis leaves after solid-phase extraction. | for hplc analysis of cardenolide glycosides of digitalis lanata the separation of the main compounds from other constituents is useful. an improved method for doing this, based on solid-phase extraction on a c18 modified poly(styrene-divinylbenzene) polymer, is described. the presented assay permits quantitative estimation of more than 50 cardenolides in about 2 mg of dried leaf powder of digitalis lanata with high speed and accuracy. | 1993 | 8445005 |
separation of cardiac glycosides by micellar electrokinetic capillary electrophoresis. | the separation of mixtures of primary and secondary cardiac glycosides by micellar electrokinetic capillary electrophoresis modified by cyclodextrins, urea and sodium cholate proved to be suitable for the determination of these hydrophobic compounds. it was possible to distinguish the two anomeric cardenolides glucodigifucoside and glucodigiglucomethyloside with all three buffer systems. electropherograms of crude plant cell extracts from digitalis lanata were obtained with this method. | 1993 | 8486748 |
drugs for atrial fibrillation. digoxin comes from digitalis lanata. | 1996 | 8611904 | |
extraction and quantitation of digoxin and acetyldigoxin from the digitalis lanata leaf via near-supercritical methanol-modified carbon dioxide. | an extraction process is reported that employs a near-supercritical mixture of co2 and meoh to extract the cardiac glycoside, digoxin, from the digitalis lanata leaf. the method development of the sample preparation procedure is presented in detail, and reasons for trends that occur in the natural products extraction are given. | 1996 | 8759168 |
quantitative determination of cardiac glycosides in digitalis lanata leaves by reversed-phase thin-layer chromatography. | an analytical method for the determination of cardiac glycosides in digitalis lanata leaves by reversed-phase thin-layer chromatography (rp-tlc) was developed. the procedure consisted of extraction of dry leaf powder with 50% methanol and clean-up by sep-pak cartridges prior to rp-tlc analysis. rp-tlc was performed on an octadecylsilyl bonded silica gel plate, using a developing solvent of acetonitrile-methanol-0.5 m nacl (1:1:1) for primary glycosides and acetonitrile-methanol-0.5 m nacl (12:7: ... | 1996 | 8916557 |
isolation of cardenolides from a brazilian cultivar of digitalis lanata by rotation locular counter-current chromatography. | cardenolides from a brazilian cultivar of digitalis lanata were isolated by rotation locular countercurrent chromatography (rlcc), employing dichloromethane-methanol-water (5:6:4, v/v) as the solvent system. highly pure lanatoside c was obtained from the digitalis lanata hydromethanolic extract, pre-purified either by silica gel or reversed-phase chromatography. | 1996 | 9008858 |
21'-di-dehydro-deacetyllanatoside c, a biotransformation product of deacetyllanatoside c from senescent shoot cultures of digitalis lanata. | feeding deacetyllanatoside c to senescent shoot cultures of digitalis lanata resulted in the formation of a new product, which was isolated by semi-preparative hplc. the molecular structure was elucidated by means of hplc-mass spectrometry and nmr as 21'-di-dehydro-deacetyllanatoside c. | 1997 | 9055448 |
cell type and spatial location dependence of cytoplasmic viscosity measured by time-resolved fluorescence microscopy. | information on the cell type and spatial location dependence of cytoplasmic viscosity would be very useful in understanding some of the processes occurring in the cell. for this purpose, fluorescent dye kiton red (sulforhodamine b) was loaded into a variety of cells such as swiss 3t3 fibroblasts, human mononuclear cells, sarcoma-180 tumor cells, chinese hamster ovary cells, plant cells from digitalis lanata, stamen hair cells of tradescantia, and guard mother cells of allium cepa. space-resolved ... | 1997 | 9143317 |
extracellular polysaccharides produced by suspension-cultured cells from digitalis lanata. | extracellular polysaccharides (ecp) were isolated in yields of up to 4 mg/ml from the culture media of suspension-cultured cells from digitalis lanata ehrh. ecp content was increasing continuously over the first ten days of cultivation and then stayed constant until day 20. ecp were fractionated by ion-exchange chromatography into two neutral and one acidic fractions. further fractionation was achieved by gel-permeation chromatography (gpc). one neutral fraction was separated into two distinct f ... | 1997 | 9342950 |
purification and characterization of lanatoside 15'-o-acetylesterase from digitalis lanata ehrh. | lanatoside 15'-o-acetylesterase (lae) from in-vitro-cultivated cells of digitalis lanata ehrh. was isolated and partially sequenced. the enzyme was extracted with citrate buffer from acetone dry powder. it was purified in a two-step chromatographical procedure including phenyl sepharose hydrophobic interaction chromatography followed by cm sepharose cation-exchange chromatography to more than 330 mumol.s-1.(g protein)-1. sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) of the ... | 1998 | 9530881 |
cardenolide 16'-o-glucohydrolase from digitalis lanata. purification and characterization. | a three-step chromatographic procedure was developed for purification of cardenolide 16'-o-glucohydrolase (cgh) from digitalis lanata ehrh. leaves, including phenyl-sepharose hydrophobic interaction chromatography followed by sp-sepharose cation exchange and q-sepharose anion-exchange chromatography. starting with acetone dry powder the purification resulted in an 760-fold enrichment of cgh. molecular weight, substrate specificity, ph optimum and temperature stability of cgh were determined. ant ... | 1998 | 9640670 |
contamination of botanical dietary supplements by digitalis lanata. | 1998 | 9738088 | |
stress-induced expression of cyclophilins in proembryonic masses of digitalis lanata does not protect against freezing/thawing stress. | using proembryonic masses (pems) of digitalis lanata erh., it was demonstrated that cold, hormonal or osmotic stress, which increased freezing tolerance during cryopreservation, induced an increasing level of two peptidyl-prolyl-cis/transisomerases (ppiases). the difference in pi (9.2 +/- 0.2 and 9.5 +/- 0.2, +/- sd; n = 3) allowed the separation of the two enzymes by free-flow isoelectrophoresis. both were inhibited by cyclosporin a and thus belong to the cyclophilin family of ppiases. the enzy ... | 1999 | 10420652 |
delta(5)-3beta-hydroxysteroid dehydrogenase from digitalis lanata ehrh. - a multifunctional enzyme in steroid metabolism? | delta(5)-3beta-etaydroxysteroid dehydrogenase (delta(5)-3beta-hsd; ec 1.1.1.145), an enzyme converting pregn-5-ene-3beta-ol-20-one (pregnenolone) to pregn-5-ene-3,20-dione (isoprogesterone), was isolated from the soluble fraction of suspension-cultured cells of digitalis lanata l. strain viii. starting with acetone dry powder the enzyme was purified in three steps using column chromatography on fractogel-tsk deae, hydroxyapatite and sephacryl g-200. fractions with highest delta(5)-3beta-hsd acti ... | 1999 | 10550629 |
structure-specificity relationship of cardiac glycosides as a substrate for glucohydrolase ii. | cardenolide glucohydrolase ii (cgh ii) is a cardenolide-specific glucohydrolase obtained from digitalis lanata leaves. we investigated the structure-specificity relationship of several cardenolide disaccharides as a substrate for cgh ii. conformation analysis of the substrates was performed using molecular mechanics calculations. the sugar chain conformation of two inert glycosides was significantly different from that of the other glycosides. the other two glycosides, which were weak substrates ... | 2000 | 10726855 |
isolation and characterization of two acyl-coa-binding proteins from proembryogenic masses of digitalis lanata ehrh. | two acyl-coa-binding-protein (acbp) isoforms were isolated from proembryogenic masses of digitalis lanata ehrh. by column chromatography and preparative hplc. the acbps had molecular masses of 9926 and 9997 da, respectively. partial sequence data indicated high similarity to each other and to acbps of other plant species such as ricinus communis, brassica napus and arabidopsis thaliana. the isolated acbps bound palmitoyl-coa with high affinity as determined by isoelectric-point shift. | 2000 | 10787064 |
haploid plants regenerated from androgenic cell cultures of digitalis lanata. | androgenic callus was obtained from cold treated anthers and pollen of digitalis lanata. the callus was mixoploid and contained haploid, diploid and tetraploid cells as shown by impulse cytophotometry. haploid cell lines were selected by colony cloning. they were unstable and selection had to be repeated every 1-2 months. mixoploid shoot cultures were derived from embryogenic haploid cell lines via somatic embryos. haploid shoots were selected by explanting shoot tips. the shoots showed wide var ... | 2000 | 10821049 |
cloning and functional expression in escherichia coli of a cdna encoding cardenolide 16'-o-glucohydrolase from digitalis lanata ehrh. | a clone of cardenolide 16'-o-glucohydrolase cdna (cgh i) was obtained from digitalis lanata which encodes a protein of 642 amino acids (calculated molecular mass 73.2 kda). the amino acid sequence derived from cgh i showed high homology to a widely distributed family of beta-glucohydrolases (glycosyl hydrolases family 1). the recombinant cgh i protein produced in escherichia coli had cgh i activity. cgh i mrna was detected in leaves, flowers, stems and fruits of d. lanata. | 2000 | 11092916 |
functional replacement of the essential ess1 in yeast by the plant parvulin dlpar13. | a functionally pin1-like peptidyl-prolyl cis/trans isomerase (ppiase(1)) was isolated from proembryogenic masses (pems) of digitalis lanata according to its enzymatic activity. partial sequence analysis of the purified enzyme (dlpar13) revealed sequence homology to members of the parvulin family of ppiases. similar to human pin1 and yeast ess1, it exhibits catalytic activity toward substrates containing (thr(p)/ser(p))-pro peptide bonds and comparable inhibition kinetics with juglone. unlike pin ... | 2001 | 11118437 |
[solubility and dissolution rate of digoxin from digitalis lanata drug extracts] . | the influence on solubility and dissolution rate was investigated for digoxin as a model drug with a very low solubility in water. the investigations were carried out with different fractions of extracts from leaves of digitalis lanata. these fractions differ in the composition of concomitant compounds. the solubility of digoxin from the extract fractions is increased up to 42 times, with considerable differences between the fractions. the solubility depends on the weight of the extract fraction ... | 2000 | 11189870 |
selective cytotoxicity evaluation in anticancer drug screening of fractionated plant extracts. | chosen to reflect biodiversity in a phylogenetic sense, 100 fractionated plant extracts were screened in vitro for cytotoxicity following extraction and fractionation (polypeptide isolation). of these 100 extracts, 30 were selected and then characterized preliminarily for antitumor potency and mode of action by testing them on two cell lines and primary cultures of human tumor cells. on the basis of cytotoxicity potency, 10 of the extracts were further characterized for anticancer activity in 10 ... | 2002 | 12230887 |
cvadh1, a member of short-chain alcohol dehydrogenase family, is inducible by gibberellin and sucrose in developing watermelon seeds. | to understand the molecular mechanisms that control seed formation, we selected a seed-preferential gene (cvadh1) from the ests of developing watermelon seeds. rna blot analysis and in situ localization showed that cvadh1 was preferentially expressed in the nucellar tissue. the cvadh1 protein shared about 50% homology with short-chain alcohol dehydrogenase including aba2 in arabidopsis thaliana, stem secoisolariciresinol dehydrogenase in forsythia intermedia, and 3beta-hydroxysterol dehydrogenas ... | 2003 | 12552151 |
systemic induction of the biosynthesis of terpenic compounds in digitalis lanata. | a bacterial screening was carried out in the rhizosphere of two digitalis species, d. thapsi and d. parviflora, both at the vegetative stage and at flowering. a total of 480 isolates were characterised at genus level, bacillus being the dominant genera in all cases. fifty percent of the bacillus strains isolated from each species were analysed by pcr-rapds. at 85% similarity, 12 groups separated for d. thapsi and 18 for d. parviflora. one strain of each group was selected for biological assay on ... | 2003 | 12685026 |
[choline in the leaves of digitalis purpurea and digitalis lanata]. | 1952 | 13031573 | |
[therapy of cardiac insufficiency with the whole glycoside of digitalis lanata]. | 1952 | 13034171 | |
[pharmacology of pure glycoside digoxin obtained from digitalis lanata]. | 1953 | 13083205 | |
[clinical experiences with a new and effective preparation from digitalis lanata: digoxin]. | 1953 | 13083206 | |
[vagus potential of strophantus, digitalis lanata and purpurea glycosides in the electrodermatogram]. | 1953 | 13126001 | |
some observations on the effect of different drying methods on the glycoside content of the leaves of digitalis lanata. | 1954 | 13184424 | |
[clinical experience in the therapy of cardiac insufficiency, including serious cases, with the new digitalis lanata preparation panlanat awd]. | 1954 | 13188048 | |
[the use of c-glucoside of digitalis lanata in pediatrics]. | 1954 | 13190677 |