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[isoenzymes of phosphorylase from skeletal muscles of cyclostomata and raw-boned fish].separation and partial purification of isoenzymes of phosphorylase b from skeletal muscles of lamprey (lampetra) and carp (cyprinus carpio) was carried out. isoenzyme i was adsorbed on deae cellulose and eluted by kcl; isoenzyme ii was not adsorbed on deae cellulose. a number of kinetic characteristics of phosphorylase of the coldblooded were determined, e. g. km values for glucose-1-phosphate, glycogen and amp; ki for glucose-6-phosphate; stability towards denaturation (heating and effect of ur ...20131112
further observations upon the hemoglobin systems of thermally-acclimated freshwater teleosts: pumpkinseed (lepomis gibbosus), white sucker (catostomus commersoni), carp (cyprinus carpio), goldfish (carassius auratus) and carp-goldfish hybrids. 19764279
synaptic transmission from photoreceptors to bipolar and horizontal cells in the carp retina.the most important observations in the present study can be summarized as follows: 1. in horizontal and bipolar cells, blocking chemical transmission caused membrane potential changes of the same polarity as the responses to illumination in each cell type. 2. acceleration of transmitter release from the receptor terminals depolarized the horizontal cells, which is the opposite polarity to the light response. 3. stimulation by transretinal current flowing from receptor side to vitreous side depol ...19767385
the survival of carp (cyprinus carpio) outside water.the possible use of the swimbladder of carp as auxiliary respiratory organ when the animals are exposed to air has been studied. after exposure to air, blood ph and po2 and mean swimbladder o2 concentration decrease and blood pco2 increases in normal animals. exposure to air after ligation of the pneumatic ducts results in increases in blood ph and pco2 and decreases in blood po2 and swimbladder carbon dioxide concentration. possible reasons for these observations are discussed.19768845
studies on glycogenolysis in carp liver: evidence for an amylase pathway for glycogen breakdown. 197610130
on the allosteric transition between the structures of high and low ligand affinity in carp hemoglobin.the variation of magneto-optical rotatory dispersion with ph for carp deoxyhemoglobin in the presence and absence of inositol hexaphosphate was interpreted as a ph-induced allosteric transition between the structures of high and low ligand affinity (the r and t states in terms of the two state model of cooperativity). increasing the ph from 6 to 11 causes a decrease in the fraction of molecules in the t state from 1 to 0.65. in the absence of inositol hexaphosphate the ph dependence of this frac ...197610981
[relationship between the characteristics of the acid stimulus and the impulse activity of medulla oblongata neurons in cyprinus carpio carp].in experiments on immobilized carps, by means of extracellular recordings, studies have been made of the neuronal activity of the facial lobe in the medulla evoked by stimulation of skin chemoreceptors of the mouth opening with hydrochloric and cytric acids. responses of the neurons to these stimuli exhibit both similarities and differences. composing across-neuron patterns, some aspects of coding of quality and intensity of chemical stimulus in fish were elucidated. particularly it was shown th ...201317250
molecular evolution of glycogen phosphorylase and aminotransferases of vertebrate muscle tissue.comparative biochemical studies on phosphorylase b, aspartate aminotransferase and alanine aminotransferase in muscles of various vertebrates (the lamprey lampetra fluviatilis, dogfish squalus acanthias, rays dasyatis pastinaca and raja clavata, teleosts scorpaena porcus, spicara smaris, esox lucius, tinca tinca, abramis brama, lucioperca lucioperca, cyprinus carpio, salmo ischchan, frog rana temporaria, tortoise testudo horsfieldi) revealed some peculiarties of their molecular evolution. it was ...197717251
organization of purpine degradation in the liver of a teleost (carp; cyprinus carpio l.). a study of its subcellular distribution.carp liver was fractionated by differential and density gradient centrifugation and assayed for enzymes of purine catabolism. while urate oxidase is an exclusively peroxisomal enzyme, only a very small percentage of the enzymes xanthine oxidase, allantoinase and allantoicase is associated with subcellular or ganelle fractions. there is no general purine catabolizing subcellular compartment. there is some but not yet conclusive evidence for the assumption that urate oxidase is a membrane bound en ...197718664
preparation of peroxisomes from carp liver by zonal rotor density gradient centrifugation.peroxisomes from carp liver can be separated by isopycnic density gradient centrifugation in sucrose. without reaching complete sedimentation equilibrium, the purification by this method is quite successful. there is a 40-fold enrichment of catalase, the peroxisomal marker, with a total yield of 27%. no pretreatment of animals is necessary for separation from lysosomes, which, besides high fragility, show lower buoyant densities than peroxisomes. the enzyme content of carp liver peroxisomes is s ...197823363
effects of variations in the perfusate on the erg and discharge of ganglion cells in carp retina. 199925193
thermodynamic investigations of proteins. iv. calcium binding protein parvalbumin.the conformational transitions of calcium binding protein parvalbumin iii from carp muscle were studied by scanning calorimetry, potentiometric titration and isothermal calorimetric titration. changes of gibbs energy, enthalpy and partial heat capacity were determined. the removal of calcium ions by edta is accompanied by 1) a heat absorption of 75 +/- 10 kj per mole of the protein, 2) a decrease in the gibbs energy of protein structure stabilisation of about 42 kj mol-1 and 3) a decrease in the ...197827259
[effect of total and prolonged starvation on the acid soluble nucleotides in carp liver].the composition of the acid soluble nucleotide pool of carp liver was determined by column chromatography, and its changes were followed during prolonged total fast. total prolonged starvation caused substantial changes in the distribution of hepatocyte free nucleotides. eleven months of starvation caused a reduction in the concentration of di- and triphosphate nucleosides as well as a diminution of the oxido-reduction of the hepatocyte. only pyrimidine nucleoside monophosphates remained at conc ...197827315
further evidence suggesting that the slow phase in protein unfolding and refolding is due to proline isomerization: a kinetic study of carp parvalbumins. 197830472
[effect of different concentrations of hco3- and co2 in aquarium water on metabolism in the cyprinus carpio l. organism].the level of pco2 and bicarbonates in blood and tissues of cyprinus carpio l. increases with concentration of carbonic acid (hco3- and pco2), the other indices of the hydrochemical composition being constant. the value of blood ph is unchanged. in fish with a higher content of carbonic acid in blood the amount of lactate, pyruvate, oxaloacetate, malate, citrate, alpha-ketoglutarate, ammonia, glutamin, free amino acids, total and residual nitrogen rises. simultaneously the content of urea increas ...201537623
subcellular distribution and some properties of alanine aminotransferase in striated muscles of the crayfish, trout, carp, frog, pigeon and rabbit.subcellular distribution and some physicochemical properties of alanine aminotransferase in striated muscles of the crayfish, trout, carp, frog, pigeon and rabbit were studied. it was established that: (1) alanine aminotransferase activity in all mentioned animals occurred almost entirely in the cytosolic fraction of the muscles. total activity and activity per mg protein were highest in crayfish and pigeon muscles and lowest in carp and trout muscles. (2) the ph optimum for the muscles of homoi ...197938468
carp hemoglobin. v. effects of ph and p6-inositol on autooxidation.autooxidation of carp hemoglobin has been measured from 4--25 degrees c with and without p6-inositol. the rate was accelerated by the increase of proton concentration and/or the addition of p6-inositol. as the rate increases the kinetics become more complex. in acidic media with p6-inositol, the autooxidation is initially rapid which becomes slower subsequently but eventually proceeds very fast. the activation energy for autooxidation is about 19 kcal. mol-1. the complicated kinetics are partly ...197941592
a rhabdovirus isolated from carps in hungary. experimental infection of carps and resistance of the virus.from carps showing the symptoms of acute infectious dropsy, a virus was isolated for the first time in hungary. on the basis of physicochemical and serological tests and electron microscopy, the virus was identified as spring viraemia carp virus and assumed to have a primary role in the acute form of infectious dropsy known so far as a bacterial disease.197944429
in vitro metabolism of permethrin isomers by carp and rainbow trout liver microsomes. 201444718
[studies of the antibody heterogeneity in carp (cyprinus carpio l.). i. electrophoretic and isoelectric spectra of anti-dnp-antibodies].carp igm as well as carp anti-dnp-antibodies migrate electrophoretically very well as a diffuse band into polyacrylamide gel of large pore size. the isoelectric spectra of the carp anti-dnp-antibodies are heterogeneous and show bands in the pi-range of 4.0 to 6.4. the activity of focused anti-dnp-antibodies could be demonstrated in the pi range between 5.4 to 6.4 even in high antibody dilutions. the investigated structural heterogeneity of the anti-dnp-antibodies of carp is a further proof for t ...197945163
carp (cyprinus carpio) muscle fructose 1,6-bisphosphatase: purification and some properties.1. fructose 1,6-bisphosphatase from the white muscle tissue of the carp, cyprinus carpio l. was purified. 2. the mol. wt of the enzyme was 145,000. its subunit mol. wt was ca. 35,000. 3. the enzyme exhibited neutral ph optimum, activation by monovalent cations, and temperature-dependent allosteric amp inhibition. 4. carp muscle fructose 1,6-bisphosphatase was 10- to 30-fold more sensitive to amp inhibition than the carp liver enzyme. 5. the carp muscle enzyme was less sensitive to amp inhibition ...197745527
species specificity of thromboplastin. a phylogenetic study.having studied the influence of thromboplastin preparations derived from cold and warm-blooded species on the plasma of 12 vertebrates (carp, frog, turtle, hen, rabbit, rat, mouse, guinea-pig, ground squirrel, dog, sheep and man) we have established that among the species far from each other phylogenetically, the phenomenon of species specificity could be demonstrated in general. it was found that the extrinsic coagulation system measured by quick times can well be activated in every examined sp ...197554309
regulatory effect of temperature and antigen upon immunity in ectothermic vertebrates. i. influence of hapten density on the immunological and serological properties of penicilloyl-carrier conjugates.sodium penicillin was conjugated to sheep erythrocytes and optimal quantities, added to a 5% srbc suspension, were determined for haemagglutination (12-5 mg/ml) and for haemolysis (50 mg/ml) using carp antibodies and carp complement. the epitope density on the bsa molecule was gradually increased, when increasing amounts of sodium-penicillin were added to a constant quantity of bsa, until a maximum of about thirty penicilloyl groups were bound. low conjugates, having less than seven haptenic gro ...197661940
tectal afferent neurons identified by the retrograde hrp method in the carp telencephalon. 197769474
neuronal architecture of on and off pathways to ganglion cells in carp retina.bipolar, amacrine, and ganglion cells of carp retina, stained intracellularly with procion yellow, can be divided into types a and b, according to the destination of terminals and dendritic trees in the inner plexiform layer (sublamina a and b, respectively). type a cells showed hyperpolarizing, or off, responses and type b cells depolarizing, or on, responses. carp thus resembles cat in the basic organization of on and off pathways in the retina.197773223
relation between carp (multiple-sclerosis associated) agent and multiple sclerosis.it is suggested that the carp (multiple-sclerosis associated and scrapie associated) agents may be the v-r.n.a. form of slow (d.n.a.) viruses which are the aetiological agents of the two diseases.197673745
polymorphism of parvalbumins and tissue distribution: characterization of component i, isolated from red muscles of cyprinus carpio l.the component i isolated from carp red muscle has been characterized as a true parvalbumin, fairly different from carp parvalbumins described so far. the protein is antigenically related to the parvalbumin iii from pike, which belongs to the so called parvalbumin lineage alpha. immunological investigations on the location of the various carp parvalbumins reveal genuine variation in the pattern of these proteins according to organ and type of muscular tissue.197875026
pituitary cytology of the indian carp labeo rohita (ham.).in the adenohypophysis of labeo rohita eight cell types could be identified on the basis of their different tinctorial response to various staining techniques. the rostral pars distalis has 3 types of cells. type 1 is stained with erythrosin, orange g, and acid fuchsin. type 2 cells are lead hematoxylin positive and give an amphiphilic reaction in response to tri and tetrachrome techniques. type 3 cells are stainable with pas, ath, ab, af, and aniline blue and are distributed in an area comprise ...197776451
retinal ganglion cells in the crucian carp (carassius carassius). i. size and number of somata in eyes of different size.ganglion cell somata were drawn, measured and counted in flat-mounted crucian carp and goldfish retinas stained with cresyl violet or methylene blue. some diameter histograms suggest that the ganglion cells can be divided into two populations with overlapping soma sizes: a large group of small cells and a small group of large cells, the latter constituting 2.5-5% of all ganglion cells. with increasing distance from the optic disc the mean soma diameter increases while the ganglion cell density d ...197876639
isolation and characterization of the three light chains from carp white muscle myosin.myosin from carp white muscle contains two mol of "dtnb" light chain (mol. wt 17 500 daltons) and two mol of "alkali" light chains (mol. wt 25 000 and 16 400 daltons). the three light chains have been isolated in pure state and characterized by electrofocusing, ultraviolet absorption, amino acid analysis and tryptic peptide mapping. our results show a great homology between the two carp alkali light chains whereas lc2 seems chemically more different. but the homology of lc1 and lc3 is not so ext ...197779371
horizontal cells of the carp retina: golgi impregnation and procion-yellow injection.application of two methods for the selective staining of neurons, golgi impregnation and intracellular marking with procion yellow, has revealed the anatomical arrangements of the horizontal cells in the carp retina. there are two basic horizontal-cell types, those with axons and these without. the former can be subdivided into three groups on the basis of the pattern of branching of the dendrites. these three structural groups are also functionally distinct, as shown by the electrical recording ...197883194
molecular adaptability of carp myosin: a study of some physico-chemical properties and their comparison with those of rabbit myosin.during thermal inactivation, the addition of as low as m urea resulted in the reduction of delta g identical to barrier of the inactivation of carp myosin ca2+-atpase, whereas that of rabbit myosin remained unaffected. in the absence of urea, a four-hour incubation of carp myosin was accompanied by the release of light chains at 30 degrees c, a value 10 degrees c lower than that for rabbit myosin. electron micrographs revealed that carp myosin forms artificial thick filaments, which were uniform ...197993883
[structural and immunochemical studies of carp (cyprinus carpio l.) immunoglobulin. v. tryptic fragments of carp (cyprinus carpio l.) immunoglobulin m].carp igm, isolated from normal serum is more sensitive to trypsinization compared to a human myeloma protein igmgo. under the same conditions (treatment with trypsin at 56 degrees c for 30 min) carp igm was degraded to small, mostly dialysable peptides to a larger extent than igmgo. in both cases the fragmentation resulted in immunoelectrophoretically pure fab mu and fc mu fragments. the fab mu fragments of human igm (yield: 20% of used igm material) had a molecular weight of 54,000, the fc mu f ...197995144
[a comparative study of the stereoselectivity of cholinoreceptors].muscarine-sensitive cholinoreceptors (m-chr) of higher vertebrates exhibit high stereoselectivity which is also revealed with respect to enantiomers of a very potent muscarinomimetic methyldilvasen (f-2268), the stereospecific index (ssi) being about 100. m-chrs in the neuronal membrane of the gastropod mollusc planorbarius corneus and in the hearts of the bivalve molluscs mercenaria stimpsoni and anadara broughtoni are highly sensitive to methyldilvasen (10(-9)-10(-10) m), but their sensitivity ...201495838
[the "staircase" phenomenon in the myocardial activity of fishes and amphibians].studies have been made on chrono- and inotropic effects in the heart of the frog rana temporaria and fishes silurus glanis and cyprinus carpio during stationary and transitional stimulation. in contrast to data in the literature, no interspecific differences were found in changes of the amplitude of cardiac contractions induced by changes in rhythmic stimulation.200895845
[host-virus relationship during experimental spring viremia in carp].one-summer-old virus-free carps produce both circulating interferon and neutralizing antibodies when stored at +20 degrees c and injected intraperitonealy with 10(5), 10(6), 10(7) p. f. u. of spring viremia of carp virus per fish: kinetics and intensity of interferon production are maximum for the highest virus imput (fig.) and neutralizing antibodies are present in most of the fish 2 1/2 months post infection (table).197896984
[immunocytology of alpha-endorphin positive hypothalamic fibers of carassius auratus l. and cyprinus carpio l].an immunocytological study of the basal hypothalamus of carassius auratus and cyprinus carpio with an alpha-endorphin antiserum shows the different nerve processes belonging probably to the immunoreactive cells of the lateral part of the n.l.t. according to their pathways, two main fibre tracts have been distinguished: many fluorescent fibres converge towards the pituitary gland into which they enter, other fibres run caudally through the tuber towards the nucleus tuberis inferior (baumgarten an ...199997002
effects of locally applied chemicals on transretinal potential and horizontal cells in the isolated carp retina. ii. calcium-chelator. 200198753
[antibiotic sensitivity of the bacterial flora in hemorrhagic septicemia in carp]. 197899867
[speed of in vitro reassociation of gonadotropin subunits in a teleost fish, the carp (cyprinus carpio l.)].purified gonadotropin subunits of a teleost fish, the carp, reassociated in vitro, according to a second order rate reaction. the rate constant was temperature dependent. compared to ovine lh, the q10 was about three times less and the rate constant much greater (160 times for instance at 20 degrees c). the possible physiological significance of these differences is underlined.1978102442
[localization of immunoreactive sites with anti met-enkephalin and anti alpha-endorphin sera in carp brain].in the brain of the carp an anti met-enkephalin serum reveals some telencephalic fibres, about half of the n.p.o. cells and furthermore a subependymal zone of nervous tissue close to the third ventricle of the superior hypothalamus and thalamus. these structures do not react with an anti alpha-endorphin serum, which however reveals cells of the lateral n.l.t. and the corresponding fibres.1979111832
[immunocytologic differentiation of hypophyseal innervation in carp using anti met-enkephalin and anti-alpha-endorphin serums].the immunocytological reactions with anti-met-enkephalin and anti-alpha-endorphin reveal two types of fibres in the intermediate lobe of the carp. their pathways through the adenohypophysis and the localization of their terminals are different. the anti-met-enkephalin positive fibres terminate mainly in a zone close to the proximal pars distalis; however some fibres terminate deeper in the intermediate lobe. the anti-alpha-endorphin positive fibres penetrate mainly in the central and ventral par ...1979111863
gill sphaerosporosis in the common carp and grasscarp. 2006112845
[organoleptic and microbiological examination of suffocated carp stored under various conditions].a process of dead market carp deterioration was studied in relation to conditions of the environment and the fish treatment by organoleptic and microbiological methods. a scheme is presented for fish assessment according to the symptoms of deterioration and the limits of carp storability under the given conditions of storing are determined. the results of the microbiological examination were not in keeping with the degree of deterioration of the fish.2002117603
[the suitability of some chemical methods for evaluation of freshness of stored suffocated carp].for the assessment of the degree of meat freshness of stored unbleeded carp some simple chemical methods were tested to be used as auxiliary criteria for veterinary decisions regarding the edibility. under the experimental conditions where the suffocated carp were stored at cooling and room temperatures in water or in the air, differences were determined in the followed criteria between fresh and deteriorating fish which were statistically insignificant for most indicators. in view of requiremen ...1979117604
[change in ribose levels in the meat of stored carp].to study the changes in ribose levels in carp meat during storage, total ribose soluble in acids was determined, ribose precipitating by barium hydroxide (free ribose), the ratio of these two values, and determination from the alkaline extract of zinc sulphate was also used. already in freshly suffocated carp relatively high levels of acid-soluble ribose were found as well as free ribose. the even increase of pentose in the alkaline extract of zinc sulphate allows to use the procedure for the ev ...1979117605
pesticide-induced alterations in the oxygen uptake rate of a freshwater major carp labeo rohita. 1979119624
trout mortalities as a result of streptococcus infection.excessive mortalities were experienced in the bigger rainbow trout (salmo gairdneri) at an efficiently managed trout farm. all tests for known toxins in the feed and water proved to be negative. a faecal streptococcus which belongs to the lancefield group d but which could not be identified as belonging to any of the recognized species within this group, was isolated from the spleens, livers and kidneys of affected fish. pathogenicity studies with this organism proved it to be highly fatal to tr ...1979121769
[regulations of igm immune response. iv. effect of the hapten: carrier ratio and the nature of the carrier on the intrinsic and functional affinity of carp dnp-antibodies].carp anti-dnp antibodies were raised by various dnp-carrier conjugates. their intrinsic affinity (k0) to monovalent e-dnp-l-lysine and functional affinity (df) for binding to the multivalent dnp-t4 bacteriophages were determined. the functional affinity of antibodies elicited by t-cell-dependent dmpn-hsa (n = 3, 15, 33) is relatively high (kf):1010-1012 m-1). these kf values increase more than k0 during the immune response. the functional affinity is dependent on the molar knp: hsa ratio. the me ...1979121787
comparative activities of glycogen phosphorylase and gamma-amylase in livers of carp (cyprinus carpio) and goldfish (carassius auratus).1. the activity of glycogen phosphorylase in goldfish liver is fivefold greater than that in carp liver, suggesting that the enzyme may not be as important in regulating glycogenolysis in the latter species. 2. the activity of gamma-amylase is comparable in carp and goldfish liver. 3. the activity of hepatic gamma-amylase is approximately one-half that of glycogen phosphorylase in carp whereas in goldfish, the activity of gamma-amylase is less than one-sixth that of phosphorylase. hepatic gamma- ...2002122584
prevention of freeze denaturation of carp actomyosin by sodium glutamate.1) denaturation of carp actomyosin during storage at -20 degrees was studied with particular interest in the cryoprotective effect of sodium glutamate, the most cryoprotective of the compounds tested previously. 2) storage with glutamate prevented the rapid decrease in solubility, viscosity, and atpase (ec 3.6.1.3)activity of actomyosin during storage. ultracentrifugal studies suggested that aggregation occurred in the frozen state without glutamate, but that added glutamate prevented aggregatio ...1975125275
[sodium reabsorption and the membrane systems of vertebrate kidneys].sodium reabsorption is low in coldblooded animals (the lamprey lampetra fluviatilis, red salmon oncorhynchus nerka, carp cyprinus carpio, frog rana temporaria, tortoise agryonemis horsfieldi) being much higher in warmblooded ones (chicken, pigeon, rat). high level of na transport in warmblooded animals is paralleled by high activity of succinate dehydrogenase as compared with the activity of na,k-atpase. the content of monoenoic fatty acids in the glycerophosphatides from frog and tortoise kidne ...1975125513
[effect of adrenaline, glucagon and insulin on glucose metabolism in carp: influence of temperature].in carps living since 4 months at 6, 20 or 30 degrees c, epinephrine or glucagon injections produce increase in plasma glucose but affect only slightly liver glycogen: lower is the temperature, slower and longer are the effect. insulin injection induces more or less delayed hypoglycaemia according to temperature acclimatization; decrease in blood glucose is accompanied by a slight increase of glycogen in all tissues at 6 degrees c and on the contrary by a very strong depletion of this polysaccha ...2002126734
[parasitic fauna of fishes in the "chilu-chor chashma" spring (tadzhik ssr) with a constant and high water temperature].the spring chilu-chor chashma (forty four springs) is situated in the south of tadzhikistan. in this water body water temperature is constant in any season of the year (18 to 20 c). ichthyofauna is represented by three species: varicorhinus heratensis steindachneri, alburnoides bipunctatus eichwaldi and cyprinus carpio. as a result of parasitological studies 20 species of parasites were recorded from 129 fishes. the parasite fauna of the fishes was investigated twice-in summer and in autumn. it ...2007130595
[postmortem changes and the anatomico-histologic structure of some organs of the carp]. 2002130762
control of energy metabolism in fish white muscle.concentrations of key metabolites were determined in carp white muscle before exercise and after maximal activity. it was found that the concentration of atp decreases by about 65%, adp decreases slightly, and amp remains unchanged. consequently, the level of the free adenylate pool decreases. simultaneously there is an increase in the concentration of imp and nh4+. the increase in imp level and the decrease in adenylate pool are essentially in 1:1 stoichiometry, a result showing that the adenyl ...1976131493
[effects of insulin on the protein metabolism of carp liver].studies on protein metabolism in carp liver under influence of insulin injections have been carried out. protein turn-over is significantly reduced by insulin; in fact, half-life of tritiated proteins is increased to twice the controls value. furthermore, electron microscopic studies of nucleolus suggest an enhancement of proteosynthesis. our findings, similar to those generally described in mammals are in good accordance with the rise of protein amount. with regard to the strong drop of carpe l ...1975132256
effect of colchicine and prednisolone upon the peptidergic neurosecretory product in cyprinus carpio l.after a treatment with physiological doses of colchicine and prednisolone, the rapid transport of the neurosecretory granules in the preoptico-neurohypophyseal tract of cyprinus carpio l. is blocked. this fact is manifested by the accumulation of neurosecretory granules and of hering's bodies at the level of fibres of preoptico-neurohypophyseal tract, and on the contrary, by the significant reduction of neurosecretory product in the nerve fibres of the neurohypophysis. the neurotubules which par ...2013134270
comparison of the composition and properties of natural actomyosin from carp (cyprinus carpio) and rabbit skeletal muscle. 1977137097
[integral proteins of the sarcoplasmic reticulum of rabbit and carp skeletal muscles]. 1977140043
[new species of infusoria of genus scyphidja (ciliata, peritricha) from fishes in kolkhid-anatolia area basins].two new species of the genus scyphidia are described. the species were collected in 1964-1966 on fishes from waters of the kolkhida-anatolijsky area. s. baninae was found on the skin of alosa caspia palaeostomi and s. doliaris - on the skin of cyprinus carpio.1977143644
regulatory proteins from dorsal muscle of the carp. 1977144727
[spring disease of the carp in the fish ponds of crimea]. 1977145071
calcium sensitivity of mantle muscle of squid.ca2+-sensitivity of squid muscle proteins was studied by competition tests between squid actin and carp myosin on squid myosin b and by cross combination tests between squid myosin and actin and between carp myosin and squid thin filament fraction. the results suggest that the regulatory system of the squid mantle muscle is actin-linked as in skeletal muscle rather than myosin-linked. this view is supported by the presence of troponin-like components on sds-disc electrophoresis of squid thin fil ...1978149115
two calcium regulation systems in squid (ommastrephes sloani pacificus) muscle. preparation of calcium-sensitive myosin and troponin-tropomyosin.the ca-regulatory system in squid mantle muscle was studied. the findings were as follows. (a) squid mantle myosin b (squid myosin b) was ca-sensitive, and its ca-sensitivity was unaffected by addition of a large amount of rabbit skeletal myosin (skeletal myosin) or rabbit skeletal f-actin (skeletal f-actin). (b) squid myosin was prepared from the mantle muscle. it showed a heavy chain component and two light chain components in the sds-gel electrophoretic pattern: the molecular weights of the l ...1978153902
another case of human gnathostomiasis in indonesia.a male gnathostoma spinigerum was removed from the abdominal wall of a 29-year-old woman, who was in the habit of eating "naniura", a dish containing raw carp (cyprinus carpio). after the removal of the worm she still had pain in her chest and noticed intermittent swelling of her right lower arm. probably the patient still had one or more gnathostoma worms migrating in her body.1978155313
peculiarity of temperature sensitivity of carp & rabbit myosins. 1979157328
[new species of the genus myxobolus (myxosporidia, myxobolidae) from the gill filaments of the carp]. 2007160029
nature of adenosine triphosphatase accelerating peptide from hydrolysate of fur seal muscle.ultrafiltered fur seal muscle hydrolysate was divided into eleven fractions by gel filtration on sephadex g-15. one of the fractions (fraction g9) accelerated the atpase activity of carp myosin b to a rate about two-fold faster than that of the control. fraction g9 showed a single ninhydrin spot in its silica gel thin layer chromatograph, and gave a positive test for tryptophan by the p-dimethylaminobenzaldehyde method, while tests for tyrosine, and for arginine were negative. the ion exchange a ...1979160912
fluorescent and spin label probes of the environments of the sulfhydryl groups of porcine muscle adenylate kinase.the environments of the two sulfhydryl groups of procine muscle adenylate kinase have been investigated by chemical modification reactions. the results indicate that the environments of the two-sh groups of procine muscle adenylate kinase are markedly different and that substrates induce conformational changes in the enzyme in the region of the sulfhydryl groups. the fluorogenic reagent 7-chloro-4-nitrobenzo-2-oxa-1, 3-diazole (nbd-chloride) reacts specifically with the -sh groups of the enzyme ...1975163231
crystalline adenylate kinase from carp muscle.1. the concentration of adenylate kinase in carp muscle is about 0.3 mg/g. an improved isolation procedure makes use of a dilute solution of the substrates, atp and amp, to elute the enzyme from a phosphocellulose column in overall yields of 60% before crystallization. by the hexokinase--ph-stat assay the specific activity is 3550 units/mg. the preparation has been found to be essentially homogeneous by dodecylsulfate gel electrophoresis, isoelectrofocusing and gel filtration. 2. the molecular w ...1975164348
structure-function relationships and mode of replication of animal rhabdoviruses.recently accumulated knowledge allows more precise comparison of the structural (and possibly evolutionary) relationships of several different animal rhabdoviruses: vesicular stomatitis virus, rabies virus, kern canyon virus, and spring viremia of carp virus. each virus is composed primarily of a glycoprotein, an rna-associated nucleoprotein, and one or two membrane proteins. vesicular stomatitis virus group viruses contain lesser amounts of two additional distinct polypeptides, ns and l. the se ...1975165494
effects of aspartate and glutamate on the bipolar cells in the carp retina. 2002166508
vesicles carrying nuclear material in mature cyprinus carpio erythrocytes. 1975166858
proprioceptive motor control in fish respiration.the response of single respiratory neurones in the medulla oblongata of carp to short twitches of individual respiratory muscles were analysed. the muscle contractions were obtained through automatic electrical stimulation and could be consistently elicited in a predetermined phase relation to the ventilatory cycle. the results show that, apart from nerve cells which take part in long-term processing of proprioceptive information from several sources, neurones also exist which possess the proper ...1975168305
[exophthalmogenic effect of thyrotropin releasing hormone (trh) in the animal experiment].exophthalmos has been induced in carp by injecting thyrotropin releasing hormone (trh) (pyro-glutamyl-histidyl-prolinamide) into the coelom. this effect was dose-dependent (dose range 5-750 mug). it was significantly potentiated by prior administration of beta-1 minus 24 coricotropin (synacthen, ciba) and inhibited by prednisclone. no significant increase was obtained with 2-phenylalanine-8-lysire-vasopressin (octapressin, sandoz). the results show that in the fish model, trh exerts an exophthal ...1975168634
inactivation of rabbit, pig, and carp adenylate kinases by n6-o- and p-fluorobenzoyladenosine 5'-triphosphates.n6-o- and p-fluorobenzoyladenosine 5'-triphosphates (iiic and iic, respectively) have been synthesized as potential adenosine 5'-triphosphate (atp) site-directed reagents for enzymes. iic and iiic were substrates of yeast hexokinase; neither they nor the corresponding adp derivatives inactivated yeast hexokinase or rabbit pyruvate kinase. iic rapidly inactivated rabbit and carp muscle adenylate kinases; the effect is probably atp site directed because n6-benzoyl-atp did not inactivate and was a ...1975172123
characterization of "pasteurella" piscicida isolated from white perch and cultivated yellowtail.the morphological, physiological, and biochemical characteristics of twelve "pasteurella" piscicida strains isolated from white perch and yellowtail are described and the present uncertain taxonomic status of the organisms is discussed. the organisms isolated were gram-negative rods showing bipolar staining and pleomorphism. no spores or flagella were observed. they were non motile and viscid colonies were formed. growth was observed in a temperature range of 20 to 30 c and the salinity range of ...1975172679
effects of external ions on the synaptic transmission from photorecptors to horizontal cells in the carp retina.1. intracellular recordings were made from cones and horizontal cells of the isolated carp retina and the mechanisms of synaptic transmission from cones of horizontal cells were studied by changing the ionic composition of the external medium. 2. cones were depolarized and their light responses enhanced in low ca high-mg medium. in the same medium, in which chemical transmission is suposed to be blocked, horizontal cells were hyperpolarized and their light responses disappeared. 3. when the syna ...1975173833
cerebrospinal fluid-contacting neurons, ciliated perikarya and "peptidergic" synapses in the magnocellular preoptic nucleus of teleostean fishes.the magnocellular preoptic nucleus of fishes (anguilla anguilla, amiurus nebulosus, cyprinus carpio, carassius auratus, ctenopharyngodon idella, cichlasoma nigrofasciatum) has been studied by light and electron microscopy. two kinds of neurons were found: a) large, electron-dense, gomori-positive cells with moderate acetylcholinesterase (ache) positivity which contain granulated vesicles of 1400 to 2200 a (in average 1600 to 1800 a), adn b) small, strongly ache-positive, electron-lucent neurons ...1987174818
the distribution of pyrophosphatidic acid in nature.the occurrence of a novel phospholipid, pyrophosphatidic acid, in the lipid extracts of yeasts (23 species), bacteria (e. coli), algae (chlorella), mammalia (human, rabbit, guinea pig, and mouse), insect (cockroach), fish (carp), mollusc (clam), and spermatophyta (spinach) was investigated. pyrophosphatidic acid was found exclusively in the lipid extracts of several kinds of yeast species, but not in other normal living species (animals, plants, and microorganisms) so far investigated. all of th ...1975175047
evidence for species-specific substrate-site-directed inactivation of rabbit adenylate kinase by n6-(6-iodoacetamido-n-hexyl)adenosine 5'-triphosphate.adenosine 5'-triphosphate (atp) derivatives bearing iodoacetylamino-n-alkyl substituents [(ch2)nnhcoch2i] on n6 were synthesized as potential atp-site-directed irreversible inhibitors of adenylate kinases from rabbit, pig, and carp muscle. when n was 5 no enzyme was progressively inhibited (inactivated) by 1 mm inhibitor under the test conditions (6 h at 0 degrees); when n was 6 the rabbit enzyme was 76% inactivated by 0.79 mm inhibitor whereas the pig and carp enzymes were unaffected by 2.76 mm ...1976187748
electron paramagnetic resonance study of carp methemoglobin.the g anisotropy of the epr spectra of carp azidomethemoglobin is found to be ph-dependent, whereas, the spectra of human azidomethemoglobin are not. the two hemoglobins have the same g values at alkaline ph values. crystal field analysis yielded values of 2.25 and 3.31, respectively, for the rhombic distortion, v/lambda, and the tetragonal distortion, delta/lambda. the spin orbit coupling constant is lambda. at ph 4.0 the values of v/lambda and delta/lambda for carp azidomethemoglobin became 1. ...1977190229
cellular distribution of sarcoplasmic calcium-binding proteins by immunofluorescence.specific antibodies against carp paravalbumin, crayfish calcium binding protein and crayfish arginine kinase were used for indirect immunofluorescence localization of the respective proteins. simultaneous staining of the same muscle sections with human serum containing anti-actin autoantibodies served as a probe to identify the isotropic band. parvalbumin appears to be evenly distributed in carp white muscle. the crayfish calcium binding protein however shows a distinct localization, in the isot ...1977192701
ca2+-dependent regulation of cyclic-amp phosphodiesterase by parvalbumin.carp parvalbumin has been shown to activate rat brain phosphodiesterase in a ca2+-dependent manner. the concentration of ca2+ required for half-maximal stimulation is 1.4 x 10(-7) m, whereas rat testis ca2+-dependent regulator (cdr) of phosphodiesterase required 1.2 x 10(-6) m ca2+. the difference in the slopes of the two curves demonstrated that the activation induced by parvalbumin was not the result of a small contamination by cdr. in addition, it has been shown that ca2+ binding to parvalbum ...1977195956
[glucose-6-phosphatase activity of the pia mater of several representative vertebrates].studies have been made on the glucose-6-phosphatase activity in the pial matter of the lamprey lampetra fluviatilis, carp cyprinus carpio, frog rana temporaria, tortoises emys orbicularis and testudo horsfieldi, hen, rabbit and cat. the data obtained not only confirmed earlier observations on extremely high activity of the enzyme in lamprey's brain, but also demonstrated high activity of glucose-6-phosphatase in the pial matter of other vertebrates, especially in tortoises.1977197748
identification of respiratory motor neurons in the carp and determination of their firing characteristics and interconnections.motor neurons of the respiratory muscles of the carp, located in the medulla oblongata, are identified with a signal averaging technique. analysis of the firing characteristics of these neurons provides data on their activity patterns under different circumstances. the results show that, on the one hand, the number of spikes a motor neuron fires per respiratory cycle depends on the respiratory intensity, and can even be as low as one action potential per respiration. on the other hand, with cons ...1977200306
[inhibition of corticotropic function by administration of gaba in carp (cyprinus carpio l.)].the intracardiac injection (50 microgram/g) of gaba to carps clearly inhibits the rise of the plasma cortisol level which occurs usually in response to the blood sampling manipulations. the inhibition starts thirty minutes after injection and lasts till the end of the experiment two hours later. the hypothesis that this neurotransmitter has a regulatory function on the corticotrop cells, especially on the excretion of acth has to be considered.1977200377
influence of quaternary structure of the globin on thermal spin equilibria in different methemoglobin derivatives.we have measured the paramagnetic susceptibilities of sperm whale azide metmyoglobin and of carp azide, thiocyanate, and nitrite methemoglobin in the quaternary oxy (r) and deoxy (t) structures between about 300 and 90 k, using a new sensitive superconducting magnetometer. we have also measured the pressure dependence of the high- and low-spin optical absorption bands of azide metmyoglobin and of carp azide methemoglobin in the r and t structures between 1 and 2000-4000 atmospheres. at low tempe ...1990210801
ovarian neoplasia in ornamental hybrid carp (nishikigoi) in japan.massive abdominal enlargements were observed in 21 adult carp 4--6 years old living in the breeding ponds of carp fisheries in northern japan. the abdominal enlargement rapidly increased, and the affected fish died within a few months. at necropsy, single or multiple tumors, 1.5--25 cm (average, 10.6 cm) in diameter, were found in the abdominal cavity. ovarian tissue was identified close to the tumors in 15 cases, and there was no evidence of anatomic continuity between the tumors and other visc ...1978212993
effect of polarisation of horizontal cells on the on-centre bipolar cell of carp retina. 1987213734
uptake, distribution, and elimination of sodium linear alkylbenzene sulfonate and sodium alkyl sulfate in carp.uptake, distribution, and elimination of labeled surfactants, 35s-labeled sodium laurylbenzenesulfonate (35s-c12-las) and 35s-labeled sodium lauryl sulfate (35s-c12-as), were investigated in carp exposed to a low concentration level of the respective solutions found in river water in japan by whole-body autoradiography and liquid scintillation counting. whole-body autoradiograms showed that the fish absorbed 35s-c12-las and 35s-c12-as from the solutions principally through gills and that the sur ...1984215398
effects of exogenous neuraminidase on unit activity in frog spinal cord and fish optic tectum.the effect of exogenous neuraminidase on spontaneous and evoked synaptic activity was investigated following injection of the enzyme into frog spinal cord and into cord and into the carp optic tectum. 1. injection of enzyme into spinal cord segments caused a significant increase of motoneuron activity in the corresponding spinal nerve, which lasted for 7--31 min. 2. the amplitude of postsynaptic evoked potentials in the optic tectum was increased after neuraminidase injection up to 200% of contr ...1979215438
l-aspartate: evidence for a role in cone photoreceptor synaptic transmission in the carp retina.a number of putative neurotransmitter substances and their antagonists were applied to the carp retina while intracellular recordings from l-type cone horizontal cells were made. of all the substances tested, l-aspartate was found to be the most potent agent in depolarizing these horizontal cells in dark-adapted, partially light-adapted, and co2+-treated retinas. furthermore, dl-alpha-aminoadipate, an l-aspartate antagonist, blocked the effects of both the endogenous photoreceptor transmitter an ...1981217017
resonance raman and epr of nitrosyl human hemoglobin and chains, carp hemoglobin, and model compounds. implications for the nitrosyl heme coordination state.we report the joint resonance raman (rr) and electron paramagnetic resonance (epr) study of five- and six-coordinate nitrosyl heme model compounds and of the titled nitrosyl hemoproteins. both epr and rr spectra fall into two types which, in the models, correspond to five- and six-coordinate nitrosyl hemes. however, neither rr nor epr spectroscopy is highly sensitive to the nature of the bond between a nitrosyl heme and a coordinated nitrogenous base, nor do the results of one technique uniforml ...1979220232
inability of parvalbumin to function as a calcium-dependent activator of cyclic nucleotide phosphodiesterase activity.the calcium-sensitive phosphodiesterase-stimulating activity sometimes associated with parvalbumin preparations is due to contaminating (less than 0.1%) amounts of carp muscle cdr (calcium-dependent regulator)-like protein. this protein can be resolved from parvalbumins by sephadex g-75 chromatography and has many characteristics of the cdr. parvalbumin itself causes a nonspecific stimulation of phosphodiesterase at all calcium concentrations which, in the presence of cdr, can cause an apparent ...1982220239
isolation and chemical composition of sarcolemmal membranes from carp skeletal muscle. 1969223664
studies on the mechanisms underlying horizontal-bipolar interaction in the carp retina.responses of on-center bipolar cells and horizontal cells were recorded simultaneously in the carp retina, and the effect of polarization of horizontal cells on the bipolar cells was studied. hyperpolarization by extrinsic current of horizontal cells elicited in the bipolar cells a hyperpolarizing response which, unlike the electrical coupling betweeen adjacent horizontal cells, was accompanied by a change in membrane conductance. the bipolar cell responses elicited by polarization of external h ...1978225825
suppression of retinal spike discharge by dipropylacetate (depakene): a possible involvement of gaba.effects of dipropylacetate (dpa) were studied on spike discharges in the isolated carp retina. (1) dpa, applied electrophoretically or by pressure-microinjection in the vicinity of the recording electrode at the inner plexiform layer, consistently activated spike discharges. (2) dpa introduced into the perfusate produced complex actions; some units responded to dpa with an initial activation followed by suppression of spike discharges. (3) when gamma-aminobutyric acid (gaba) was applied electrop ...1979226226
tactile input to the facial lobe of the carp, cyprinus carpio l. 1979227540
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