Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| the degradation of the cuticle proteins of greater wax moth larvae (galleria mellonella) by toxic proteolytic enzymes, secreted by the entomophagous fungus beauveria bassiana (author's transl). | 1976 | 8934 | |
| studies on insect bacteriolytic enzymes--ii. some physical and enzymatic properties of lysozyme from haemolymph of galleria mellonella. | 1976 | 9261 | |
| exocellular proteases of serratia marcescens and their toxicity to larvae of galleria mellonella. | out of 18 strains of serratia marcescens producing exocellular proteases the strain serratia marcescens cceb 415 was selected according to preliminary experiments. it could be shown that the train exhibits proteolytic activity reaching up to 10 tu per 1 ml of the culture filtrate in a medium with gelatine and peptone. two proteolytic enzyme could be demonstrated by means of specific inhibitors edta and diisopropyfluorophosphate: metalprotease with optimum activity at ph 7.5 and serine protease w ... | 1976 | 11154 |
| purification of free polyhedrosis virus infecting galleria mellonella l. | free galleria mellonella nuclear polyhedrosis virus was purified 4000--4500 times by tween-80 treatment and repeated sucrose density gradient centrifugation, based on the ratio of infectivity to protein content as compared to the starting virus-containing suspension. | 1977 | 17285 |
| proteins of bacillus thuringiensis delta-endotoxin crystals. | pure crystals (at least 99% purification) of sigma-endotoxin were isolated from bac. thuringiensis var. galleriae. the complete dissolution of crystals might be achieved by the increase of ph up to 12 and higher or by a combined action of s = s-reducing and denaturing agents. electrophoresis of the solubilized crystal proteins in 5% polyacrylamide gels containing 0,1% sodium dodecyl sulfate and 8 m urea reveals two major bands corresponding to molecular weights of 120000--140000 (65%) and 65000 ... | 1977 | 21000 |
| [comparative biochemical studies of polyhedral proteins of nuclear polyhedrosis viruses]. | using disc polyacrylamide gel electrophoresis, the molecular weights of polyhedral proteins of nuclear polyhedrosis viruses (npv) of porthetria dispar, mamestra brassicae, and aporia crataegi were found to be 28000 +/- 3000. it was shown that npv polyhedra of bombyx mori, galleria mellonella, p. dispar, and m. brassicae contain a protease. during dissolution of the polyhedra at ph 10,5 this protease specifically cleaves the matrix protein into 2--5 fragments. the amino acid compositions of npv p ... | 1978 | 33725 |
| [the cellular reticular structures induced by baculovirus in the lepidoptera galleria mellonella]. | induction of filamentous nuclear structures by baculoviruses are observed in the hemocytes of galleria mellonella larvae. modalities of detection of these virus-induced structures by means of sheep or rabbit globulines labelled with fluorescein isothiocyanate are described. | 1975 | 50151 |
| an iridovirus from bees. | an iridovirus, apis iridescent virus (aiv), isolated from sick adult specimens of apis cerana (hymenoptera) from kashmir, closely resembles iridescent viruses from tipula and sericesthis spp. (tiv and siv). however, aiv is only distantly related serologically to tiv and siv and is even more remotely related to several other similar viruses that were tested in tube precipitation tests with intact particles. aiv multiplies in apis mellifera, forming cytoplasmic iridescent crystalline aggregates in ... | 1976 | 58966 |
| polypeptide antibiotic 26a from bacillus subtilis. i. taxonomy and fermentative production. | in surface cultures on nk/2-sym's medium, the isolate no. 26a of bacillus subtilis from the intestinal tract of galleria mellonella larvae produced three antibacterial substances which were separated by gel filtration on sephadex g-25 column. the major bioactive compound named 26a had a close resemblance to bacitracin family of polypeptide antibiotics. two minor active compounds, i.e. a bacteriolytic enzyme with endo-beta-n-acetylmuramidglycanohydrolase (ec. 3. 2. 1. 17) activity and other unide ... | 1978 | 81595 |
| time and dose studies of the effect of cobra venom factor on the in vivo immune response in galleria mellonella to pseudomonas aeruginosa. | the induced resistance of wax moth larvae vaccinated with formolized pseudomonas aeruginosa 18 hr prior to challenge with the live organism was significantly inhibited by 0.2 to 1.0 units of cobra venom factor (cvf) per insect given 6 hr after vaccination. approximately 90% inhibition of the protective response was caused by 1.0 units cvf/insect. administration of this dose 6 hr before vaccination, with the vaccine, and 6 hr before challenge had no significant effect. the cvf effect was inhibite ... | 1978 | 99334 |
| gut flora of galleria mellonella suppressing ingested bacteria. | 1979 | 119813 | |
| ruthenium red staining of the neural lamella of the brain of galleria mellonella. | the outer surface of the neural lamella, the connective tissue ensheathing the brain, shows the ability to bind ruthenium red in the wax moth larva. ruthenium red-positive material is sensitive to neuraminidase, hyaluronidase and to some extent to phospholipase c, what suggests that the negative charge on the external surface of the neural lamella depends on the presence of the anionic groups of sialic and hyaluronic acids and phospholipids. | 1977 | 137773 |
| the ultrastructure and ultracytochemistry of the basement membrane of the galleria mellonella fat body. | the fat body lobes of galleria mellonella are surrounded by basement membrane - a fine granular layer of connective tissue. this membrane has an affinity for ruthernium red. the results obtained after treatment of the fat body with neuraminidase, hyaluronidase, phospholipase c and proteolytic enzymes suggest that glycoproteins and phospholipoproteins are constituents of this basement membrane. the basement membrane also has the ability to bind concanavalin a-peroxidase, which is associated with ... | 1977 | 137774 |
| the fragments from the 28 s ribosomal rna of galleria mellonella with unesterified uridine at the 3'-termini. | the three 3'-ends of the 28 s galleria rna are all unesterified uridines. the two 18-s products due to the primary nick of the 28 s rna have a similar 3'-terminal dinucleotide: g-uoh. all of the seven species of secondary products from the galleria 28 s rna were suggested to have unesterified uridines, in common, at the 3'-ends. these results raise the possibility that the enzyme concerned in generating the primary and secondary nicks is a uracil-specific and 5'-phosphate-forming phosphodiestera ... | 1976 | 175853 |
| supercoiled dna of nuclear polyhedrosis virus of galleria mellonella l. | supercoiled infectious dna was isolated from nuclear polyhedrosis virus infecting great wax moth galleria mellonella l.) covalently closed dna molecules constitute approximately 10--30 per cent in our preparations. these molecules dissappear during storage. electron micrographs of supercoiled and open circular molecules are presented. length of the open rings is about 50--52 micron. infectivity of different dna forms is discussed. | 1977 | 322646 |
| the encapsulation of foreign tissue implants in galleria mellonella larvae. | 1977 | 323370 | |
| macromolecular structure of nuclear polyhedrosis virus genome. | dna preparations from nuclear polyhedrosis virus (npv) of galleria mellonella l. (gml) were fractionated in high ionic strength neutral sucrose gradient. this procedure allowed a separation of supercoiled infectious dna molecules with contour length of 48--52 microns from infectious open ring dna molecule, and noninfectious linear dna molecules of the same size. in addition a heterogeneity of supercoiled dna molecules was detected. covalently closed dna molecules did not contain protein or ribon ... | 1979 | 378185 |
| electron microscopical-immunocytochemical evidence of ecdysteroids in the prothoracic gland of galleria mellonella. | fixation of prothoracic glands of galleria mellonella with a solution containing saponin permits immunocytochemical staining of the entire gland. by this means ecdysteroids were demonstrated electron microscopically to be present in the hyaloplasm and microtubules. | 1979 | 385145 |
| non-occluded particles of nuclear polyhedrosis virus infecting galleria mellonella l.: titration in vivo and in vitro. brief report. | infectivity of non-occluded particles of nuclear polyhedrosis virus infecting galleria mellonella l. was determined with bioassay technique in vivo and with plaque assay method in vitro using scld 135 (quiot) established cell culture. a plaque-forming virus unit corresponds to about 50 id50 for galleria larvae injected into hemocoel for non-cloned and 0.1-1.0 id50 for a virus isolate cloned from an infective plaque. | 1979 | 391180 |
| studies on the in vivo cellular reactions of insects: an ultrastructural analysis of nodule formation in galleria mellonella. | cellular aggregates or nodules which formed in galleria mellonella larvae in response to injections of killed bacillus cereus were examined during their first 24 hr of development. the initial aggregation stage was very rapid, occurring within 1 min, and was brought about by the release of an adhesive flocculent material by granular haemocytes in contact with the bacteria. this material, which subsequently became increasingly electron dense owing to melanin deposition, consolidated and by 5 min ... | 1977 | 408940 |
| [studies on the activation of humoral defense factors against bacteria in larvae of the wax moth galleria mellonella l]. | the change of lysozyme activity in the hemolymph of the wax moth larvae caused by vaccination is influenced by the size and the dose of the injected particles. the rate of incorporation of those particles into phagocytes has no effect on this process. the change of lysozyme activity correlats negatively with the amount of granulocytes after vaccination. there were no correlations with the amount of other types of the hemocytes, neither with the resistance of the larvae against pseudomonas aerugi ... | 1977 | 410207 |
| an ultrastructural and cytochemical study of the interaction between latex particles and the haemocytes of the wax moth galleria mellonella in vitro. | the plasmatocytes are the major phagocytic blood-cell type in the haemolymph of the wax-moth, galleria mellonella. in the present study, these cells were allowed to attach to tissue culture dishes for 1 h, rinsed and then incubated with latex beads for up to 72 h. these cells were then fixed for routine transmission electron microscopy and acid phosphatase cytochemistry. intracellular latex particles were found in tight, ill-defined phagosomes, which were often clearly associated with the golgi ... | 1979 | 466691 |
| effect of reserpine on the larval-pupal moult of the wax moth galleria mellonella l. (lepidoptera). | significant retardation of g. mellonella development was induced by reserpine injected to the last instar larvae in doses having a long-lasting effect on the neurosecretory system. | 1979 | 510494 |
| [improved methods for the mass breeding of greater wax moth larvae (galleria mellonella l.)]. | 1979 | 516927 | |
| phagocytosis-stimulating mediators in insects. | the ability of insect blood cells to ingest all kinds of synthetic particles and also a wide range of microorganisms in a very short time after injection has up to now been regarded as a phagocytic function without any humoral mediators. in a phagocytosis model with latex beads and nonhagocytosable cells of bacillus thuringiensis subtoxicus, we are able to demonstrate the existence of lymphokine-like factors, which intervene in cellular defence reactions of insect. the following results were obt ... | 1979 | 543373 |
| the neural lamella of the wax moth galleria mellonella l. (lepidoptera) brain. | 1979 | 546092 | |
| ultrastructural changes in the ovaries of the wax moth, galleria mellonella l. (lepidoptera), after treatment with reserpine. | 1979 | 546096 | |
| comparative levels of lysozyme activity in larvae and pupae of galleria mellonella after particulate and soluble materials injection. | using an assay system for lysozyme activity determination in which haemolymph was compared in activity to known standards, the quantitative shifts in the lysozyme activity level of larvae and pupae of galleria mellonella, after particulate and soluble materials injection, were compared. the level of this activity in all injected larvae was higher fro that of pupae, but the immune response of pupae was more effective in all treatments when compared with activity in the non-injected controls. furt ... | 1979 | 552308 |
| defensive reactions of galleria mellonella l. catepillars to the effect of herbicides and the nematode neoaplectana carpocapsae weiser. | 1977 | 564232 | |
| ultrastructure of the protocerebral neurosecretory cells of larval galleria mellonella, in situ and after culture of the brain in vitro. | three major groups of neurosecretory cells are described in the larval brain of galleria mellonella at two different times during the last larval instar and in larval brains after 72 hr of culture in vitro. the medial group in vivo consists of four distinct neurosecretory cell types, based on characteristic size and morphology, while the posterior and lateral groups each contain a single distinct type of neurosecretory cell. morphological differences between the same neurosecretory cells at the ... | 1977 | 610004 |
| monoamine-containing cells in the brain of galleria mellonella (lepidoptera). | 1977 | 610395 | |
| morphogenesis of the nodamura virus in the larbae of the lepidopteran galleria mellonella (l.). | the pathogenesis and morphogenesis of the nodamura virus, an insect picorna- virus which can also infect vertebrates such as newborn mice, are described in the larvae of g. mellonella. examination of thin sections of muscle, salivary and moulting glands, hemocytes adipose tissue and hypodermis of the infected larvae of g. mellonella shows in the cytoplasm the accumulation of viral particles either dispersed or in a stringlike or paracrystalline array. these arrays of virions can be within membra ... | 1978 | 626593 |
| changes in ecdysone content during postembryonic development of the wax moth, galleria mellonella: the role of the ovary. | 1978 | 631539 | |
| steroid transport through the surface of the prothoracic gland cells in galleria mellonella l. | steroid transport through the cell surface of the giant polyploid prothoracic gland cells of galleria mellonella l. was studied by an ultracytochemical method. the alkaloid digitonin, known to form a complex with all sterols having a free-oh radical in position 3, proved to be suitable for studying the interiorisation of moulting hormone precursors and the release of synthesized hormones. the results suggest that cholesterol uptake in the last larval instar occurs by macropinocytosis during the ... | 1978 | 639089 |
| ultrastructural analysis of the action of reserpine on the brain neuroendocrine system of the wax moth, galleria mellonella l., lepidoptera. | this study concerns the influence of reserpine on the fine structure of peptidergic neurosecretory cells in the pars intercerebralis of galleria mellonella, and of neurons containing smaller dense-cored vesicles (presumed to be aminergic) localized in the same area of the brain. the drug, administered in doses of 125 microgram and 250 microgram per g of insect body weight, reduces both the amount and the electron opacity of the dense-cored vesicles with a diameter of 60--80 nm in the neuronal pe ... | 1978 | 688371 |
| reprogramming and dna synthesis in galleria mellonella larval epidermal cells. | epidermal cells from ligated day-3 last instar larvae of galleria mellonella secrete a larval cuticle if exposed to ecdysone immediately following ligation; whereas, they deposit a pupal cuticle if exposed to ecdysone three to four days after ligation. during the period of reprogramming the genome in the diploid cells of the wax moth larvae no dna synthesis occurs. this is direct proof that dna synthesis is not required for reprogramming in the epidermis of galleria during larval-pupal transform ... | 1978 | 750267 |
| [study of the homology of nuclear and polysomal virus-specific rna in nuclear polyhedrosis of galleria mellonella l]. | 2006 | 765687 | |
| [fractionation and amino-acid composition of acid-soluble trypsin of inclusion bodies protein of nuclear polyhedrosis virus of moth, galleria mellonella]. | a mixture of ph 3.5-soluble trypsin peptides of inclusion bodies proteins of moth nuclear polyedrosis virus was fractionated by means of ionic exchange chromatography on dowex 50x2 and purified by paper electrophoresis-chromatography. 32 peptides are isolated, their amino acid composition being studied. | 1976 | 799518 |
| [virus disease complexes: transmissible pathological entities in invertebrates]. | virus disease complexes of galleria mellonella l. due respectively to a parvovirus with a baculovirus and a parovirus with an iridovirus have been transmitted to healthy larvae by ingestion of corpses of larvae affected by these disease complexes. the histological and cytological injuries observed are identical to those noted during the study of the initial complexes. | 1975 | 807417 |
| [occurrence and function of a proteinase inhibitor in the hemolymph of insects]. | the presence of proteinase inhibitor has been proved in the hemolymph of a number of insect species from seven different insect orders. the amount of proteinase inhibitor in the hemolymph significantly increases after injection of inactivated bacteria into the hemocoelom of galleria mellonella-larvae. moreover the larvae show an increased resistance against normally lethal concentrations of trypsin, chymotrypsin, pronase p and extracellular proteinase produced by pseudomonas aeruginosa. it is di ... | 1975 | 811027 |
| [indirect effect of the larval food of an alternative host (galleria mellonella l., lepidoptera, galleriidae) on the reproduction of an entomophagous insect (lixophaga distraeae towns, dipt. tachinidae)]. | g. mellonella reared on its natural food, beeswax and pollen, is not suitable for a continuous rearing of l. diatraeae. as a matter of fact, l. diatraeae cannot be maintained for more than 10 generations on g. mellonella because the effect of adverse factors during the larval stage progressively reduces both mating rate and female fertility. but adding vitamin e to the natural larval food of g. mellonella eliminates these reproduction disturbances of the parasite, and l. diatraeae rearing can be ... | 1975 | 813895 |
| the pathogenicity of rough strains of pseudomonas aeruginosa for galleria mellonella. | rough mutants of two strains of pseudomonas aeruginosa have been isolated by selection for resistance to virulent lipopolysaccharide-specific phages. the rough mutants fell into two classes on the basis of colonial morphology and agglutination by acriflavine and nacl. the pigment and exoenzyme-synthesizing properties of these derivatives were identical with those of the parental cultures. the rough strains were 8- to 62-fold less pathogenic for the larvae of the wax moth (galleria mellonella) th ... | 1975 | 814978 |
| effect of cobra venom factor on the in vivo immune response in galleria mellonella to pseudomonas aeruginosa. | 1976 | 815440 | |
| [reestablishment of the molt by brain implantation in the permanent larvae of galleria mellonella]. | postcephalic ligatures of larvae of galleria mellonella resulted in the production of permanent larvae. implantation of brains into such permanent larvae restored moulting. the percentage of successful reactivation of permanent larvae depended on the number of implanted brains. | 1976 | 816532 |
| [host range of drosophila melanogaster c virus among diptera and lepidoptera (author's transl)]. | the host range of the c picornavirus of drosophila melanogaster was studied, using numerous strains of drosophila together with four other genera of diptera and two species of lepidoptera. c virus was injected into the different hosts and serially passaged in them. the extracts from each passage were biologically assayed on virus free d. melanogaster. four different situations were found. 1) a high level of multiplication leading, in 45 strains of drosophilidae, to the early death of the hosts. ... | 1976 | 823856 |
| the purification by affinity chromatography of a glutathione s-transferase from larvae of galleria mellonella. | 1977 | 834106 | |
| ultrastructure of the neurosecretory cells of the pars intercerebralis of galleria mellonella (lepidoptera) after noradrenaline administration. | 1977 | 924116 | |
| the allatotropic activity of the larval brain of galleria mellonella cultured in vitro. | 1976 | 955387 | |
| a small rna virus with a divided genome from heteronychus arator (f.) [coleoperai scarabaeidae]. | some properties of a small rna virus isolated from larvae of heteronychus arator [coleoptera: scarabaeidae] are described. the virus develops in the cytoplasm of gut and fat body cells and is 30 nm in diamter. the sedimentation coefficient of virus was 137s and the buoyant density was 1-33 g/ml. the single stranded rna was isolated as two species which sedimented at 22s and 15s, corresponding to mol. wt. of 1-0 and 0-5 x 10(6) respectively. the base ratios of the two rna species were 22 s: a=29- ... | 1976 | 978185 |
| the granular cells of galleria mellonella during clotting and phagocytic reactions in vitro. | light and electron-microscopic observations of the blood-cells (hemocytes) of the wax-moth galleria mellonella showed that hemolymph coagulation was initiated by the rapid release of material from the granular cells. during incubation for short terms in vitro these cells showed progressive degranulation as material derived from the granules was discharged into the hemolymph. attempts to determine the nature of this material by staining with ruthenium red proved mainly unsuccessful. when challeng ... | 1976 | 982421 |
| the ultrastructural organization of the visual system of the wax moth, galleria mellonella: the retina. | the ultrastructural organization of ommatidial components of the retina of the moth. galleria mellonella are described from electron microscopic observations. each ommatidium is composed of 12 common retinula cells and one basal eccentric cell. the retinula cells are connected together by a desmosomal strip along their length. the rhabdom occupies the basal thirty percent of the ommatidium and can be divided into nine segments of parallel microvilli. several cells may contribute to an individual ... | 1976 | 1000589 |
| the ultrastructural organization of the visual system of the wax moth, galleria mellonella: the optic tract. | the ultrastructural organization of the axons of retinula cells of the eye of the wax moth galleria mellonella are described. the axons traverse an appreciable distance between the basement membrane of the retina and the lamina ganglionaris of the optic lobe of the brain. the optic tract was reconstructed from serial thin sections. axons emanating from a single ommatidium are closely associated together in the optic tract. adjacent cartridges fuse together to form large clusters of axons (8 to 1 ... | 1976 | 1000590 |
| comparative chemical studies of the polyherdral proteins of the nuclear polyhedrosis viruses of bombyx mori and galleria mellonella. | 1975 | 1089730 | |
| proteolytic cleavage of polyhedral protein during dissolution of inclusion bodies of the nuclear polyhedrosis viruses of bombyx mori and galleria mellonella under alkaline conditions. | 1975 | 1089735 | |
| control of corpora allata function in larvae of galleria mellonella. | 1975 | 1115805 | |
| rna synthesis in imaginal disks of galleria mellonella: effects of alpha- and beta- ecdysone and fat body in vitro. | 1975 | 1120913 | |
| eye disk differentiation in the wax moth: induction in vitro. | ecdysterone-induced differentiation of galleria mellonella compound eyes in vitro is critically dependent on the age of the donor. it is greatest in explants from young prepupae, decreases with increasing donor age, and is absent in explants from 4-6 hour pupae. failure of eye disks from pupae to differentiate in vitro seems to be the consequence of exposing them to culture conditions at a susceptible time in their development for, unlike eye disks from prepupae, they do not develop when removed ... | 1975 | 1127401 |
| polynucleotide fragments from the 28s ribosomal rna of insects. | if rna is extracted from the ribosomes which had been isolated from frozen-thawed tissue of galleria mellonella, the 28 s rna, when heated or treated with urea, dissociates into seven different species of polynucleotide fragments. they were designated as r1, r2, r3, r4, r5, r6, and r7, whose molecular weights were estimated to be 1.15x10-6, 0.75x10-6, 0.55x10-6, 0.40x10-6, 0.30x10-6, 0.25x10-6, 0.20x10-6 daltons, respectively. it is likely that r1 and r5 arise from a single nick in original 38 s ... | 1975 | 1129145 |
| ultrastructural study of the prothoracic glands of galleria mellonella l. in the penultimate last larval, and pupal stages. | the prothoracic gland (pgl) of galleria mellonella is a y-shaped, paired organ, consisting of 45-50 polyploid giant cells. the pgl cells are supplied by neurosecretory axons; release of neurosecretory granules (1000-1300 a in diameter) directly on the surface of pgl cells was frequently observed. based on ultrastructure, the last two larval instars can be divided into three phases: 1) restitutive phase immediately after moulting; 2) gradual activation in mid-intermoult as indicated by the logari ... | 1975 | 1131863 |
| ultrastructure of the prothoracic gland cells of the last instar of galleria mellonella in relation to the state of development. | the prothoracic glands of the last instar of galleria mellonella undergo characteristic alterations of their cellular fine structure closely related to cellular activity. during progressive secretory activity of the gland cells there are extensive plasmalemmal infoldings and formation of a pronounced lacunar system. mitochondria of the active cell phase are characterized by a specific increase in size and paler colour of the matrix. in contrast to the alterations, nuclei, er and golgi cisterns d ... | 1975 | 1149124 |
| glycoproteins in the basement membranes of the ovarial tube in galleria mellonella (lepidoptera). | 1975 | 1163128 | |
| concanavalin binding by the basal membrane of the ovarial tube in galleria mellonella (lepidoptera). | 1975 | 1163129 | |
| the basement membrane of the galleria mellonella fat body. | 1975 | 1163145 | |
| the multiplication of nodamura virus in insect and mammalian cell cultures. | nodamura virus multiplied in mosquito cell lines, as determined by infectivivity assays in adult honey bees (apis mellifera) and wax moth larvae (galleria mellonella). titres of more than 10-7 and 10-5 bee ld50 /ml were obtained in culture fluids of aedes albopictus and aedes aegypti cells respectively after 10 days. comparable titres were obtained after several months, during which the cultures were subdivided up to six times. nodamura virus also multiplied in bhk cells and yielded titres of 10 ... | 1975 | 1168239 |
| studies on the incorporation of carbon-14 in tissues of galleria mellonella. | 1975 | 1184238 | |
| effect of heat-killed cells of achromobacter nematophilus poinar et thomas, and the fraction (endotoxin) isolated from them on galleria mellonella l. caterpillars. | 1975 | 1192248 | |
| influence of na+, k+, mg2+ and cooling on proteosynthesis in hemocytes of galleria mellonella. | 1976 | 1248218 | |
| the secretion of collagen by insects: uptake of [3h]proline by collagen-synthesizing cells in locusta migratoria and galleria mellonella. | the uptake of [h3]proline by collagen-secreting cells of the locust, locusta migratoria, and wax-moth, galleria mellonella, has been investigated by electron autoradiography. the locust cells are around the ejaculatory duct and they secrete collagen in the young adult male, while the wax-moth cells are those which produce the dorsal mass of connective tissue on the abdominal nerve cord during the late pupal stage. the cells were exposed to [h3]proline either by injection of the [3h]proline into ... | 1976 | 1262412 |
| effect of galleria mellonella larvae preparation and honeybee products on cell cultures. | 1. the action of the extract from larvae of the great wax moth galleria mellonella l. used in russian folk medicine was studied. 2. two active components influencing the growth and morphological differentiation of cells in vitro were found. 3. the presence of these components in the extract was conditioned by consumption of honeybee products by galleria mellonella larvae. 4. cytotoxicity of honeybee products was studied. | 1992 | 1358524 |
| brain factor from galleria mellonella (lepidoptera) stimulating silk gland activity. | brain extracts from day 1-4 last instar larvae of galleria mellonella (lepidoptera) stimulate rna synthesis in cultured silk glands from day 3 last instar larvae. when the fibroin-synthesizing posterior parts of silk glands were incubated for 3 h in vitro in the presence of brain extract (0.1 brain equivalent), [3h]-uridine incorporation into rna was stimulated more than twofold. the stimulating effect of brain extract showed a dose response relationship. it is suggested that the heat-resistant ... | 1992 | 1381321 |
| storage proteins of the larval root weevil diaprepes abbreviatus (coleoptera: curculionidae): riboflavin binding and subunit isolation. | proteins present at high concentrations in hemolymph of the larval weevil diaprepes abbreviatus were previously shown to bind a synthetic coumarin, 7-amino-3-phenyl coumarin (coumarin-10). one of the two native proteins previously identified (protein i) is now shown to separate into two distinct bands (proteins ia and ib) using native gradient pore-limiting electrophoresis. the high concentration of proteins ia, ib, and ii in larval hemolymph, their disappearance from hemolymph upon pupation, an ... | 1992 | 1504322 |
| a transfer membrane method for in situ detection and quantification of trehalase. | a method for the detection and quantification of trehalase activity (ec 3.2.1.28) by immobilization to a membrane support has been developed. protein samples partly enriched for porcine and galleria mellonella wax moth larvae trehalase activities were fractionated by polyacrylamide gel electrophoresis, followed by electrophoretic transfer to pvdf membranes, and incubated in a solution containing trehalose (20 mg/ml), glucose oxidase (40 u/ml), phenazine methosulfate (0.06 mg/ml), and nitro blue ... | 1992 | 1621991 |
| induction of immunity by latex beads and by hemolymph transfer in galleria mellonella. | injection of sterile latex beads into the hemocoel of last instar larvae of galleria mellonella provoked a strong defense reaction. cellular defense by hemocytes was followed by enhanced antibacterial activity in hemolymph. latex-injected insects showed increased survival rates after a challenge injection with high doses of bacteria. factors which stimulate the production of antibacterial activity could be demonstrated soon after injection by transfer of hemolymph from preinjected to untreated l ... | 1991 | 1773849 |
| hormonal regulation of protein synthesis in the silk gland of galleria mellonella. | synthesis of the low molecular mass silk proteins of 24 and 30 kda in the last larval instar of galleria mellonella starts between 24 and 48 h; synthesis of the former protein significantly preceding that of the latter. posterior silk glands (psg) from day-1 last instar larvae are transiently sensitive in vitro to exogenous 20-hydroxyecdysone (20-he), which stimulates transcription of the 24 kda protein gene and induces transcription of the gene for the 30 kda protein. the glands from the day -3 ... | 1991 | 1796707 |
| hydrolytic degradation of juvenile hormones in haemolymph and corpora allata of galleria mellonella (l). | hydrolytic rates of juvenile hormones (jhs) i, ii and iii by the corpora cardiaca-corpora allata complex (cc-ca) and by the haemolymph of galleria mellonella remain in the same order (iii greater than i greater than ii in cc-ca and i greater than iii greater than ii in haemolymph) throughout the last larval instar. haemolymph hydrolytic activity shows peak at the end of feeding when 80 pmol jh i versus 15 pmol jh ii is degraded per 1 microliter and minute; hydrolysis rapidly declines in the apol ... | 1991 | 1799112 |
| in vitro bioassay for the brain allatotropic hormone of galleria mellonella (lepidoptera). | an in vitro sensitive bioassay for the galleria mellonella brain allatotropic hormone (atth) was developed. this assay measures the rate of juvenile hormone (jh) synthesis in corpora cardiacacorpora allata complex (cc-ca) stimulated in vitro by atth released from the brain during short-term in vitro incubation, or by atth extracted from the tissue with methanol. cc-ca of the late vith instar (vi3) larvae were used for assessment of atth. the maximum activation of test cc-ca by atth occurred at a ... | 1991 | 1813322 |
| inhibition by inorganic anions of glutathione s-transferases from insect and mammalian sources. | glutathione s-transferases 1-1, 3-3, 3-4 and 4-4 from rat liver and the major glutathione s-transferase from the wax moth (galleria mellonella) are all inhibited by several simple inorganic anions. for each of 3-3, 3-4 and the insect enzyme, the order of inhibitory potency was clo4- greater than or equal to scn- greater than i- greater than no3- greater than br-. a more limited range of anions was tested on the isoenzymes 1-1 and 4-4, but the same trend was apparent. values for ki ranged from ab ... | 1991 | 1883329 |
| conformational change of the haemolymph juvenile-hormone-binding protein from galleria mellonella (l). | a juvenile-hormone-binding protein (juvenile-hormone carrier), isolated from galleria mellonella haemolymph, was treated with trypsin, chymotrypsin, carboxypeptidase a and subtilisin. among these enzymes, only subtilisin was able to affect juvenile-hormone-binding activity of this protein. with sds/page it was shown that juvenile-hormone-binding protein, a 32-kda peptide, is first slowly converted into a 30-kda molecule, then into two or three smaller-molecular-mass species (20-25 kda), which in ... | 1991 | 1935932 |
| cane sugar factor as an inducing agent of immunity in galleria mellonella. | the injection of cane sugar factor (csf) into galleria mellonella larvae results in an immune response similar to that produced by a formalized pseudomonas aeruginosa vaccine. vaccination with csf is followed by: an increase in the ld50 of pseudomonas aeruginosa; an in vivo protective response to p. aeruginosa the development of which can be inhibited by cobra venom factor (cvf); an antibacterial activity in hemolymph 24 h after the injection of csf; the development of a transferrable immune res ... | 1990 | 2128279 |
| implication of mitochondria in the replication of nodamura virus in larvae of the lepidoptera, galleria mellonella (l.) and in suckling mice. | the replication of nodamura virus, type species of the family nodaviridae, takes place principally into the interfibrillar spaces of the sarcoplasm in close association with mitochondria in the infected muscles of both g. mellonella and suckling mice. the most striking event in infection of g. mellonella muscle cells was the aggregation and shape modification of numerous mitochondria (elongation, interdigitation, and vesiculation) at an early stage of infection. these clusters of mitochondria we ... | 1990 | 2222183 |
| restriction maps and sequence homologies of two densovirus genomes. | the genomes of junonia coenia densonucleosis virus (jcdnv) and galleria mellonella densonucleosis virus (gmdnv) were analysed by restriction endonuclease analysis and southern blot hybridization. a total of 37 and 33 restriction sites were mapped on jcdnv and gmdnv dna, respectively. bgli, haeii and bsteii were site-specific for jcdnv dna, and bglii and clai for gmdnv dna. the two genomes had nearly identical maps for several restriction endonucleases and southern blot hybridization using a tota ... | 1990 | 2230740 |
| expression of densonucleosis virus gmdnv in galleria mellonella larvae: size analysis and in vitro translation of viral transcription products. | the rna of densonucleosis virus type 1 (gmdnv), isolated from gmdnv-infected galleria mellonella larvae, was shown by northern blotting to contain five polyadenylated, viral-specific rna species with sizes of 1.8, 2.4, 3.5, 4.0, and 5.0 kb. poly(a)-containing rna from whole larvae and hybrid-selected viral rna were translated in a rabbit reticulocyte lysate system and the translation products were coelectrophoresed with proteins extracted from cscl-purified virus. all four virion-associated prot ... | 1990 | 2273284 |
| plasmid transfer between strains of bacillus thuringiensis infecting galleria mellonella and spodoptera littoralis. | to determine the possibility of plasmid transfer occurring between strains of bacillus thuringiensis in infected lepidopterous larvae, galleria mellonella and spodoptera littoralis were infected with two or more strains of b. thuringiensis and the resulting bacteria from the dead insects were examined for plasmid transfer. transfer rates of plasmids coding for crystal production and tetracycline resistance were high, reaching levels similar to those obtained in laboratory broth cultures. transfe ... | 1990 | 2383006 |
| adherence patterns and virulence for galleria mellonella larvae of isolates of serratia marcescens. | 1990 | 2405061 | |
| effects of selected carbohydrates and the contribution of the prophenoloxidase cascade system to the adhesion of strains of pseudomonas aeruginosa and proteus mirabilis to hemocytes of nonimmune larval galleria mellonella. | the adhesion of strains of pseudomonas aeruginosa and proteus mirabilis to the plasmatocytes and granular cells of nonimmune larval galleria mellonella was influenced by and varied with the type of carbohydrate. laminarin enhanced prophenoloxidase activation and bacterial adhesion to the hemocytes whereas sucrose suppressed both activities. for all other sugars there was no correlation between bacterial adhesion to the hemocytes and phenoloxidase activity. it is proposed that bacterial adhesion ... | 1989 | 2501016 |
| the hemolytic activity of galleria mellonella hemolymph. | a hemolytic activity was identified in the hemolymph of normal and immune galleria mellonella larvae. the hemolysin was active against sheep, human, guinea pig, and rabbit erythrocytes. hemolysis occurred in the presence of 0.04m edta. vaccination of the larvae with formalized pseudomonas aeruginosa increased the hemolytic activity. the increase, and subsequent decline of this activity paralleled the pattern of induced in vivo antibacterial activity that is characteristic of the insect's immune ... | 1989 | 2506085 |
| transposon mutagenesis of baculoviruses: analysis of trichoplusia ni transposon ifp2 insertions within the fp-locus of nuclear polyhedrosis viruses. | the transposable ifp2 element of trichoplusia ni was originally isolated as a host dna insertion in spontaneous fp mutants of galleria mellonella or autographa californica nuclear polyhedrosis viruses (npvs). the termini of ifp2 insertions from five independently isolated fp mutants were sequenced. in all cases ifp2 is flanked by 13-bp terminal inverted repeats and has additional inverted repeats of 19 bp in length located asymmetrically with respect to the ends of the element. insertion of ifp2 ... | 1989 | 2549707 |
| transposon mutagenesis of baculoviruses: analysis of tfp3 lepidopteran transposon insertions at the fp locus of nuclear polyhedrosis viruses. | we report the complete sequences of two representatives of the tfp3 transposable element family of the lepidopteran, trichoplusia ni. these elements were isolated as insertions mobilized from the lepidopteran host genome into two closely related nuclear polyhedrosis viruses (npv) during infection. both elements inserted within the 500-bp fp locus of the respective viral genomes (map units 36.0 to 37.0), causing a distinctive plaque morphology phenotype and the loss of a 25-kda viral-specific pro ... | 1989 | 2553540 |
| identification and distribution of a proctolin-like neuropeptide in the nervous system of the gypsy moth, lymantria dispar, and in other lepidoptera. | although the neuropeptide proctolin has important functions in many arthropods, it is reported to be absent in lepidoptera. its possible occurrence in these insects was reinvestigated by bioassays of hplc fractions and immunocytochemistry. a proctolin-like substance was recovered from the frontal and subesophageal ganglia of lymantria dispar. this substance has the same chromatographic retention time as proctolin; enzymatic degradation indicates that it is a peptide; it is bound by proctolin ant ... | 1989 | 2732362 |
| comparisons of host cell dna insertions and altered transcription at the site of insertions in few polyhedra bacilovirus mutants. | few polyhedgra (fp) mutants of autographa californica nuclear polyhedrosis virus (acnpv) and the closely related strain galleria mellonella (gm)npv have been reported which contain trichoplusia ni host cell dna sequences inserted into the viral genome between map units 35.0 and 37.7. new fp mutants are described with alterations of the hindiii-i restriction enzyme fragment (33.8 to 37.7 map units) of acnpv, either deletions of viral dna sequences or insertions of spodoptera frugiperda host cell ... | 1988 | 2829419 |
| [effect of temperature on the infectivity of the iridovirus and densonucleosis virus of blood-sucking mosquitoes]. | mosquito densonucleosis virus and iridovirus were used to study the influence of temperature on their infectivity. biological assays of mosquito densonucleosis virus were carried out in larvae of i-ii instar of aedes aegypti mosquitoes, those of mosquito iridovirus in larvae of galleria mellonella. the rate of inactivation was found to be directly dependent on the dose of the heat treatment. treatment at 60 degrees c led to complete loss of iridovirus infectivity; the virus activity declined con ... | 2010 | 2905849 |
| ultrastructural alterations of the egg chambers of galleria mellonella l. (lepidoptera) developing after reserpine administration. | in the egg chambers of the wax moth galleria mellonella l., developing after application of reserpine, a multilayered epithelium was observed through all the developmental stages. this epithelium consisted of "light" cells loaded with intracellular organelles. no "dark" cells, characteristic of the control material, were observed. all cells of the egg chambers of reserpine-treated insects were characterized mostly by an abundance of microtubules, strong vacuolation of cytoplasm, and dilation of ... | 1985 | 2938376 |
| transposon-mediated mutagenesis of a baculovirus. | spontaneous mutants of insect nuclear polyhedrosis viruses of autographa californica (acmnpv) and galleria mellonella (gmmnpv) were analyzed. these mutants produce few polyhedra in infected cells and have insertions of host cell dna. all the insertions mapped to two adjacent sites in the genome. the junctions between two host insertions and the viral dna were sequenced. one of the insertions contained a perfect 7-bp inverted terminal repeat, and had caused a direct duplication of 4 bp of viral d ... | 1985 | 2992159 |
| [transposition and reversion of mutations induced in drosophila by viral dna]. | injection of solutions of highly polymerized dna isolated from nuclear polyhedrosis virus of galleria mellonella into adult males induced with a considerable frequency visible mutations, two of which were studied in detail. they were detected in about 30 000 flies in the progeny of treated males. much less than notched-wings much greater than (ndw, chromosome 3, location 87.9, dominant) independently arose 12 times, much less than thickened-veins much greater than (thi, chromosome 2, location 71 ... | 1986 | 3006304 |
| reactions of hemocytes of immune and non-immune galleria mellonella larvae to proteus mirabilis. | immune larval galleria mellonella removed live proteus mirabilis from the hemolymph less effectively than did non-immune larvae. this was attributed to a decline in total hemocyte counts, levels of plasmatocytes and granulocytes and hemocyte adhesion capacity. immune serum possessed factors which reduced bacterial adhesion to the hemocytes. this was not due to altering bacterial surfaces but rather to irreversible suppression of hemocyte activity. | 1987 | 3109970 |
| cold-induced supernumerary moults of galleria mellonella larvae as bioassay for anti-juvenile hormone compounds. | 2008 | 3213548 | |
| synthesis and binding affinity of an iodinated juvenile hormone. | the synthesis of the first iodinated juvenile hormone (jh) in enantiomerically enriched form is reported. this chiral compound, 12-iodo-jh i, has an iodine atom replacing a methyl group of the natural insect juvenile hormone, jh i, which is important in regulating morphogenesis and reproduction in the lepidoptera. the unlabeled compound shows approximately 10% of the relative binding affinity for the larval hemolymph jh binding protein (jhbp) of manduca sexta, which specifically binds natural 3h ... | 1988 | 3335551 |
| the meisenheimer complex of glutathione and trinitrobenzene. a potent inhibitor of the glutathione s-transferase from galleria mellonella. | 1. the meisenheimer complex formed between reduced glutathione and 1,3,5-trinitrobenzene is characterised by an extinction coefficient at 470 nm of 20400 and by an association constant at ph 9.18 of 42 l.mol-1. 2. trinitrobenzene is a moderately good inhibitor of the glutathione s-transferase from larvae of the moth galleria mellonella. it acts by competition with the electrophilic substrate. at ph 7.4, it has a ki value of 10 microm. its mode of inhibition with respect to gsh appears to be non- ... | 1988 | 3342082 |
| preliminary characterization of toxins from the straw itch mite, pyemotes tritici, which induce paralysis in the larvae of a moth. | homogenates of whole mites (pyemotes tritici) paralyze larvae of the greater wax moth galleria mellonella. injection of these homogenates into larvae produces symptoms identical to those obtained by bites from female mites. since the paralytic activity is destroyed by heat and proteolytic enzymes and retained during dialysis, the toxic compounds appear to be proteins. two protein fractions which differ both in molecular weight and toxicity were found following gel filtration of whole mite extrac ... | 1988 | 3363564 |
| further characterization of an alkaline protease activity associated with iridescent virus type 6. brief report. | iridescent virus type 6 infecting galleria mellonella larvae contained an associated alkaline protease activity which appeared to be tightly bound to the virions and essentially localized on the outside of the viral particle. under alkaline conditions the virus was degraded by proteolytic cleavage of viral envelope proteins. proteolytic activity was not present in virus propagated in a permissive insect cell line or when purified from isolated larval fat-bodies instead from whole larvae. the res ... | 1986 | 3511892 |