Publications
Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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further studies concerning the toxicity of ingested ergot sclerotia (claviceps purpurea) to young and growing pigs. | in three experiments pigs were offered diets contaminated with milled ergot sclerotia. in a metabolism study 0-5 per cent (w/w) of american wheat ergot decreased urinary nitrogen losses and improved the efficiency of nitrogen retention. when early weaned piglets ingested a diet contaminated with 2-5 per cent of the ergot, feed intake was reduced and growth rate decreased. reduction in growth rate was also evident in growing pigs given a diet containing ergotised english wheat. these latter pigs ... | 1977 | 860090 |
effect of epsilon-amino caproic acid foliar spray on seed set and embryo formation in triticum turgidum l. var. durum x secale cereale. | 1977 | 880702 | |
wheat germ agglutinin. evidence for a genetic basis of multiple forms. | germ from hexaploid wheat (triticum aestivum l.) contained three forms of agglutinin separable by ion-exchange column chromatography at ph 3.8, while germ from tetraploid wheat (triticum turgidum l. (durum group)) contained only two such forms. the different number of forms, not due to protein modification occurring during the purification process, was demonstrable in commercial germ and in bran fractions containing germ from single wheat varieties. this evidence for a genetic basis of lectin mu ... | 1976 | 953023 |
evaluation of branched ear derivatives of triticum aestivum l. | the branched spikes of triticum turgidum l. have a potential of producing a high number of kernels. some of the selected lines of t. turgidum yielded up to 150 kernels per spike as compared to 60--70 kernels per spike in the common wheat cultivar chenab-70. the best of these lines were crossed with the common wheats chenab-70, mexipak, and c-271. among the lines obtained by selection from the advanced generations of the t. turgidum x chenab-70 cross, some are similar to chenab-70 with respect to ... | 2002 | 1032097 |
biochemical data bearing on the relationship between the genome of triticum urartu and the a and b genomes of the polyploid wheats. | to determine whether the triticum urartu genome is more closely related to the a or b genome of the polyploid wheats, the amino acid sequence of its purothionin was compared to the amino acid sequences of the purothionins in triticum monococcum, triticum turgidum, and triticum aestivum. the residue sequence of the purothionin from t. urartu differs by five and six amino acid substitutions respectively from the alpha 1 and alpha 2 forms coded for by genes in the b and d genomes, and is identical ... | 1988 | 3209062 |
elucidation of the b-genome donor to triticum turgidum by unique- and repeated-sequence dna hybridizations. | in vitro dna:dna hybridizations and hydroxyapatite thermal-elution chromatography were employed to identify the diploid triticum species ancestral to the b genome of t. turgidum. unique and repeated sequences from the various triticum species were separated by hybridization and thermal elution on hydroxyapatite. unique- and repeated-sequence fractions of labeled t. turgidum var. durum dna were hybridized to the corresponding fractions of unlabeled dnas of t. searsii, t. speltoides, t. longissimu ... | 1986 | 3964230 |
additional evidence implicating triticum searsii as the b-genome donor to wheat. | in vitro dna:dna hybridizations and hydroxyapatite thermal-elution chromatography were employed to identify the diploid wheat species ancestral to the b genome of triticum turgidum. 3h-t. turgidum dna was hybridized to the unlabeled dnas of t. urartu, t. speltoides, t. sharonensis, t. bicorne, t. longissimum, and t. searsii. 3h-labeled dnas of t. monococcum and a synthetic tetraploid aadd were hybridized with unlabeled dnas of t. urartu and t. searsii to determine the relationship of the a genom ... | 1984 | 6712588 |
ribosomal rna multigene loci: nomads of the triticeae genomes. | the nucleolus organizing regions (nors) on the short arms of chromosomes 1a(m) and 5a(m) of diploid wheat, triticum monococcum l., are at the most distal loci in the linkage maps of these two chromosome arms. this distal location differs from the interstitial location of the nor loci on chromosome arms 1bs of tetraploid triticum turgidum l. and hexaploid t. aestivum l., 5ds of t. aestivum and diploid ae. tauschii coss., and 5hs of barley. moreover, the barley 5hs locus is at a different location ... | 1995 | 7498776 |
nucleotide sequence of a cdna encoding the adp/atp carrier from wheat (triticum turgidum). | 1995 | 7770541 | |
new 18s.26s ribosomal rna gene loci: chromosomal landmarks for the evolution of polyploid wheats. | three new 18s.26s rrna gene loci were identified in common wheat by sequential n-banding and in situ hybridization (ish) analysis. locus nor-a7 is located at the terminal area of the long arm of 5a in both diploid and polyploid wheats. locus nor-b6 is located in n-band 1bl2.5 of the long arm of chromosome 1b in triticum turgidum and triticum aestivum. ish sites, similar to nor-b6, were also detected on the long arms of chromosomes 1g in triticum timopheevii and 1s in aegilops speltoides, but the ... | 1994 | 7924620 |
different species-specific chromosome translocations in triticum timopheevii and t. turgidum support the diphyletic origin of polyploid wheats. | triticum timopheevii ssp. timopheevii and t. timopheevii ssp. araraticum were analysed by sequential n-banding and genomic in situ hybridization. three chromosomes, 6at, 1g and 4g, were involved in at-g intergenomic translocations in all six lines analysed. these chromosomes may be derived from a cyclic translocation that is species-specific to t. timopheevii. in contrast, triticum turgidum has a species-specific cyclic translocation involving chromosomes 4a, 5a and 7b. the discovery of differen ... | 1994 | 8162322 |
application of the random amplified polymorphic dna technique for the detection of polymorphism among wild and cultivated tetraploid wheats. | development of a high-density genetic linkage map of cultivated wheats using conventional molecular markers has lagged behind the other major food crops such as rice and tomato because of the large genome size and limited levels of genetic polymorphisms. recently, random amplified polymorphic dnas (rapds) have been suggested to provide an alternative to visualize more polymorphism. for the construction of a genetic linkage map in tetraploid wheats, one can use a strategy of intersubspecific cros ... | 1993 | 8349131 |
characterization of cadmium binding, uptake, and translocation in intact seedlings of bread and durum wheat cultivars | high cd content in durum wheat (triticum turgidum l. var durum) grain grown in the united states and canada presents potential health and economic problems for consumers and growers. in an effort to understand the biological processes that result in excess cd accumulation, root cd uptake and xylem translocation to shoots in seedlings of bread wheat (triticum aestivum l.) and durum wheat cultivars were studied. whole-plant cd accumulation was somewhat greater in the bread wheat cultivar, but this ... | 1998 | 9536059 |
characterization of zinc uptake, binding, and translocation in intact seedlings of bread and durum wheat cultivars | durum wheat (triticum turgidum l. var durum) cultivars exhibit lower zn efficiency than comparable bread wheat (triticum aestivum l.) cultivars. to understand the physiological mechanism(s) that confers zn efficiency, this study used 65zn to investigate ionic zn2+ root uptake, binding, and translocation to shoots in seedlings of bread and durum wheat cultivars. time-dependent zn2+ accumulation during 90 min was greater in roots of the bread wheat cultivar. zn2+ cell wall binding was not differen ... | 1998 | 9733541 |
purification and characterization of a lipoxygenase enzyme from durum wheat semolina. | purification of a lipoxygenase enzyme from the cultivar tresor of durum wheat semolina (triticum turgidum var. durum desf) was reinvestigated furnishing a new procedure. the 895-fold purified homogeneous enzyme showed a monomeric structure with a molecular mass of 95 +/- 5 kda. among the substrates tested, linoleic acid showed the highest k(cat)/k(m) value; a beta-carotene bleaching activity was also detected. the enzyme optimal activity was at ph 6. 8 on linoleic acid as substrate and at ph 5.2 ... | 1999 | 10552472 |
uptake and retranslocation of leaf-applied cadmium (109cd) in diploid, tetraploid and hexaploid wheats. | uptake and retranslocation of leaf-applied radiolabeled cadmium (109cd) was studied in three diploid (triticum monococcum, aa), four tetraploid (triticum turgidum, bbaa) and two hexaploid (triticum aestivum, bbaadd) wheat genotypes grown for 9 d under controlled environmental conditions in nutrient solution. among the tetraploid wheats, two genotypes were primitive (ssp. dicoccum) and two genotypes modern wheats (ssp. durum). radiolabelled cd was applied by immersing the tips (3 cm) of mature le ... | 2000 | 10938828 |
isolation of a cdna encoding a granule-bound 152-kilodalton starch-branching enzyme in wheat. | screening of a wheat (triticum aestivum) cdna library for starch-branching enzyme i (sbei) genes combined with 5'-rapid amplification of cdna ends resulted in isolation of a 4,563-bp composite cdna, sbe1c. based on sequence alignment to characterized sbei cdna clones isolated from plants, the sbeic predicted from the cdna sequence was produced with a transit peptide directing the polypeptide into plastids. furthermore, the predicted mature form of sbeic was much larger (152 kd) than previously c ... | 2000 | 10982440 |
recombination in an isochromosome preferentially occurs between cis isochromatids. | an isochromosome has identical arms attached to the same centromere. at the pachytene stage of meiosis, it has four isochromatids and recombination can occur either between cis isochromatids (attached to the same half-centromere) or trans isochromatids (attached to different half-centromeres). normally such recombination cannot be detected because all four chromatids are homogenetic (arose from misdivision of a centromere to which genetically identical sister chromatids were attached). we isolat ... | 2000 | 11072794 |
chromosome structure of triticum timopheevii relative to t. turgidum. | the chromosome structure of four different wild populations and a cultivated line of triticum timopheevii (2n = 28, atatgg) relative to triticum turgidum (2n = 28, aabb) was studied, using genomic in situ hybridisation (gish) and c-banding analysis of meiotic configurations in interspecific hybrids. two wild accessions and the cultivated line showed the standard c-banding karyotype. the other two accessions are homozygous for translocation 5at/3g and translocations 1g/2g and 5g/6g. gish analysis ... | 2000 | 11195344 |
meiotic metaphase i pairing behavior of a 5bl recombinant isochromosome in wheat. | a recombinant isochromosome i5blrec of wheat was developed with one arm and the proximal 36% of the other arm of chinese spring (cs) origin and the distal 64% of the recombined arm of triticum turgidum subsp. dicoccoides origin. the i5blrec provides an unusual opportunity to analyze the role of the centromere or arm heterozygosity in chromosome prealignment and synapsis during meiosis. in monosomic condition, the i5blrec formed a ring univalent in 86.8% of the pollen mother cells (pmcs) at meiot ... | 2000 | 11196130 |
molecular characterization of gene sequences coding for protein disulfide isomerase (pdi) in durum wheat (triticum turgidum ssp. durum). | the organisation of the durum wheat genomic sequence (3.5 kb) coding for protein disulfide isomerase (pdi), deduced by comparison between genomic fragments and cdna sequences (1.5 kb) isolated from immature caryopses, is described. the gene structure consists of ten exons and nine introns. the presence of consensus sequences involved in splicing, such as intron-exon junctions and branchpoint, has been observed and discussed. although the deduced wheat pdi amino acid sequence exhibited an overall ... | 2001 | 11255017 |
production of durum wheat substitution haploids from durum x maize crosses and their cytological characterization. | the objective of this study was to investigate the effect of individual durum wheat (triticum turgidum l.) chromosomes on crossability with maize (zea mays l.) and to cytologically characterize the haploids recovered. fourteen 'langdon' (ldn) d-genome disomic substitution lines, a ldn ph mutant (ph1b ph1b), and normal 'langdon' were pollinated with maize pollen. after pollination, hormonal treatment was given daily for up to 14 days. haploid embryos were obtained from all lines and were aseptica ... | 2001 | 11269349 |
anther culture-derived regenerants of durum wheat and their cytological characterization. | anther culture is being increasingly used in cereal crop improvement both as a source of haploids and for inducing new genetic variation. we studied the androgenetic ability and regenerability of 10 cultivars of durum wheat (triticum turgidum l., 2n = 4x = 28; aabb), using three different growth conditions and four media. from a total of 86,400 anthers cultured, 324 plants were obtained: 248 green and 76 albino. genotype, growth condition, and media significantly affected anther response and cal ... | 2006 | 11336230 |
phytosiderophore release in aegilops tauschii and triticum species under zinc and iron deficiencies. | using three diploid (triticum monococcum, aa), three tetraploid (triticum turgidum, bbaa), two hexaploid (triticum aestivum and triticum compactum, bbaadd) wheats and two aegilops tauschii (dd) genotypes, experiments were carried out under controlled environmental conditions in nutrient solution (i) to study the relationships between the rates of phytosiderophore (ps) release from the roots and the tolerance of diploid, tetraploid, and hexaploid wheats and ae: tauschii to zinc (zn) and iron (fe) ... | 2001 | 11432925 |
characterization of a mitotic mutant of durum wheat. | an ethyl methanesulfonate-induced mitotic mutant of durum wheat (triticum turgidum l. var. durum; 2n = 4x = 28) was found. we have characterized the mutant to determine the mechanism of abnormal cell division and to test for temperature effects on abnormal cell division. stained root-tip meristems and pollen mother cells were studied with brightfield, phase contrast, and immunofluorescence microscopy. abnormal cells included metaphase cells with a multiple of the normal complement (8x = 56, or 1 ... | 2001 | 11448039 |
remobilization of cadmium in maturing shoots of near isogenic lines of durum wheat that differ in grain cadmium accumulation. | cadmium accumulation in grain of durum wheat (triticum turgidum l. var. durum) represents a concern to consumers. in an effort to understand the regulation of cd accumulation in maturing grain, the remobilization of 109cd applied to stem and flag leaves was examined in two near-isogenic lines that differ in grain cd accumulation. absorbed 109cd was primarily retained in the labelling flap (50-54% and 65-80% for stem and flag leaves, respectively). cadmium exported from the stem flap initially (3 ... | 2001 | 11457907 |
genome composition, stability and fertility of hexaploid alloploids between triticum turgidum var. carthlicum and leymus racemosus. | hexaploid alloploids between the tetraploid wheat triticum carthlicum and the perennial tetraploid leymus racemosus were analysed for chromosome composition and cytogenetic stability. gish analysis showed different lines to have from 11 to 16 leymus chromosomes. the alloploids showed a relatively high frequency of univalents in meiotic metaphase and of aneuploid plants and hence they are not stable. the seedset is lower than in wheat, but high enough to secure a safe propagation and preservation ... | 2001 | 11525068 |
evaluation of chemical and technological characteristics of new lines of triticum turgidum ssp dicoccum. | triticum durum ssp dicoccum also called farro in italy is gaining more and more interest from farmer due to its commercial potentiality. in this paper, thirty lines f6 of farro obtained by crossing the cvs. triticum turgidum ssp durum ofanto and simeto and t. turgidum ssp dicoccum molise population were studied. they were evaluated with special reference to chemical composition and technological characteristics in order to select genotypes suitable for the production of food commodities. the sto ... | 2001 | 11534466 |
the synaptic behaviour of the wild forms of triticum turgidum and t. timopheevii. | different wild allopolyploid species of triticeae show extensive bivalent formation at zygotene while a considerable number of multivalents is present in cultivated polyploid wheats. to study the chromosome behaviour at early meiotic stages in wild forms of tetraploid wheats triticum turgidum and t timopheevii (2n = 4x = 28) we have analysed the synaptic pattern in fully traced spread nuclei at mid- and late zygotene and at pachytene of wild accessions of these species. the mean number of synapt ... | 2001 | 11550884 |
direct estimation of mutation rate for 10 microsatellite loci in durum wheat, triticum turgidum (l.) thell. ssp durum desf. | 2002 | 11752198 | |
the effect of plant height on tan spot on durum wheat in southern saskatchewan. | tan spot [caused by pyrenophora tritici-repentis (died.) drechs.] is the most important leaf disease of durum wheat (triticum turgidum l. var. durum). most of the durum cultivars in western canada are of conventional height but development of shorter cultivars is desirable. changes in microenvironment resulting from a height reduction might cause greater leaf spot development. the effect of plant height on tan spot of durum wheat was determined in five pairs of genotypes near-isogenic for plant ... | 2002 | 11756267 |
genotypic variation in tetraploid wheat affecting homoeologous pairing in hybrids with aegilops peregrina. | the ph1 gene has long been considered the main factor responsible for the diploid-like meiotic behavior of polyploid wheat. this dominant gene, located on the long arm of chromosome 5b (5bl), suppresses pairing of homoeologous chromosomes in polyploid wheat and in their hybrids with related species. here we report on the discovery of genotypic variation among tetraploid wheats in the control of homoeologous pairing. compared with the level of homoeologous pairing in hybrids between aegilops pere ... | 2001 | 11768203 |
water deficit during root development: effects on the growth of roots and osmotic water permeability of isolated root protoplasts. | the effect of low water potentials on root growth of flax (linum usitatissimum l. cv. ariane), rape (brassica napus l. de candolle, cv. bristol), hard wheat (triticum turgidum l. cv. cham1) and soft wheat (triticum aestivum l. cv. ritmo) was studied by measuring the osmotic water permeability (pos) of root protoplasts and the protein abundance of pip1 and pip2 aquaporins. these different species require more or less water, the most sensitive to water deficit being flax and rape. ritmo, is a cult ... | 2002 | 11855644 |
genes encoding plastid acetyl-coa carboxylase and 3-phosphoglycerate kinase of the triticum/aegilops complex and the evolutionary history of polyploid wheat. | the classic wheat evolutionary history is one of adaptive radiation of the diploid triticum/aegilops species (a, s, d), genome convergence and divergence of the tetraploid (triticum turgidum aabb, and triticum timopheevii aagg) and hexaploid (triticum aestivum, aabbdd) species. we analyzed acc-1 (plastid acetyl-coa carboxylase) and pgk-1 (plastid 3-phosphoglycerate kinase) genes to determine phylogenetic relationships among triticum and aegilops species of the wheat lineage and to establish the ... | 2002 | 12060759 |
transcripts of vp-1 homeologues are misspliced in modern wheat and ancestral species. | the maize (zea mays) viviparous 1 (vp1) transcription factor has been shown previously to be a major regulator of seed development, simultaneously activating embryo maturation and repressing germination. hexaploid bread wheat (triticum aestivum) caryopses are characterized by relatively weak embryo dormancy and are susceptible to preharvest sprouting (phs), a phenomenon that is phenotypically similar to the maize vp1 mutation. analysis of vp-1 transcript structure in wheat embryos during grain d ... | 2002 | 12119408 |
a fertile amphiploid between durum wheat (triticum turgidum) and the x agroticum amphiploid (agropyron cristatum x t. tauschii). | agropyron (gaertn) is a genus of triticeae which includes the crested wheatgrass complex, i.e. a. cristatum (l.) as representative species containing the p genome. this species is an important source for increase the genetic variability of both durum and bread wheat. among the possible interesting features to be introgressed into wheat are resistance to wheat streak mosaic virus, rust diseases, and tolerance to drought, cold and moderate salinity. by crossing tetraploid wheat (triticum turgidum ... | 2001 | 12152332 |
identification of a microsatellite on chromosome 7b showing a strong linkage with yellow pigment in durum wheat (triticum turgidum l. var. durum). | the objective of this study is to identify qtls linked to yellow pigment content in durum wheat. a durum-dicoccoides genetic linkage map was constructed using 124 microsatellites, 149 amplified fragment length polymorphism (aflps), and six seed storage proteins (ssp) in a population of 114 recombinant inbred lines (f8). the population has been obtained from a cross between a durum cultivar omrabi5 and triticum dicoccoides600545 and backcrossed to omrabi5. the map consists of 14-durum chromosomes ... | 2001 | 12152344 |
the effects of chromosomes 3a and 3b on resistance to fusarium head blight in tetraploid wheat. | fusarium head blight (fhb) caused by fusarium graminearum is one of the most destructive diseases of wheat in areas where the weather is warm and humid after heading. previous studies indicate that the level of resistance to fhb varies not only among wheat cultivars but also among some of their wild relatives. no accession, however, has yet been identified to be completely immune to fhb among the gramineae. it is known that durum wheat (triticum turgidum l. conv. durum) is consistently more susc ... | 2001 | 12152348 |
chromosome composition, stability and fertility of alloploids between triticum turgidum var. carthlicum and thinopyrum junceiforme. | alloploids, most of them hexaploid, from crosses between tetraploid wheat, triticum carthlicum, and the perennial tetraploid thinopyrum junceiforme were analysed for chromosome composition, stability and fertility using genomic in situ hybridization and meiotic analysis. the alloploids differed in their total number of chromosomes, 38-47 + telocentrics in "hexaploids" and 54 and 56 in "octoploids", and also in their number of thinopyrum chromosomes (8-15). translocations, mostly robertsonian one ... | 2002 | 12184490 |
hyperosmotic stress induces formation of tubulin macrotubules in root-tip cells of triticum turgidum: their probable involvement in protoplast volume control. | treatment of root-tip cells of triticum turgidum with 1 m mannitol solution for 30 min induces microtubule (mt) disintegration in the plasmolyzed protoplasts. interphase plasmolyzed cells possess many cortical, perinuclear and endoplasmic macrotubules, 35 nm in mean diameter, forming prominent arrays. in dividing cells macrotubules assemble into aberrant mitotic and cytokinetic apparatuses resulting in the disturbance of cell division. putative tubulin paracrystals were occasionally observed in ... | 2002 | 12198194 |
transport interactions between cadmium and zinc in roots of bread and durum wheat seedlings. | field studies have shown that the addition of zn to cd-containing soils can help reduce accumulation of cd in crop plants. to understand the mechanisms involved, this study used 109cd and 65zn to examine the transport interactions of zn and cd at the root cell plasma membrane of bread wheat (triticum aestivum l.) and durum wheat (triticum turgidum l. var. durum). results showed that cd2+ uptake was inhibited by zn2+ and zn2+ uptake was inhibited by cd2+. concentration-dependent uptake of both cd ... | 2002 | 12207664 |
genetic mapping of polyphenol oxidase in tetraploid wheat. | pasta colour is one of the main factors influencing pasta quality. it is the product of a desirable yellow component, an undesirable brown component and, under some drying conditions, a red component. the brown colour depends on enzymatic and chemical factors. polyphenol oxidase (ppo; e.c. 1.14.18.1) is one of the enzymatic factors. it is mainly localised in the peripheral part of the wheat kernel, and is involved in the oxidation of endogenous wheat phenolic compounds resulting in the productio ... | 2002 | 12378236 |
studies on the origin and evolution of tetraploid wheats based on the internal transcribed spacer (its) sequences of nuclear ribosomal dna. | in this study, the internal transcribed spacer (its) sequences of nuclear ribosomal dna in the tetraploid wheats, triticum turgidum (aabb) and triticum timopheevii (aagg), their possible diploid donors, i.e., triticum monococcum (aa), triticum urartu (aa), and five species in aegilops sect. sitopsis (ss genome), and a related species aegilops tauschii were cloned and sequenced. its1 and its2 regions of 24 clones from the above species were compared. phylogenetic analysis demonstrated that aegilo ... | 2002 | 12582618 |
aflp and pedigree-based genetic diversity estimates in modern cultivars of durum wheat [ triticum turgidum l. subsp. durum (desf.) husn.]. | a substantial amount of between and within cultivar genetic variation was detected in all the 13 registered modern canadian durum wheat ( triticum turgidum l. ssp. durum (desf.) husn.) cultivars based upon amplified restriction fragment polymorphism (aflp). of the approximately 950 detected aflp markers, only 89 were polymorphic, with 41 between cultivars whereas the remaining 48 showed polymorphism within at least one cultivar. the ancestry of canadian durum wheat cultivars was traced back to 1 ... | 2002 | 12582707 |
identification of the wheat seed protein cm3 as a highly active emulsifier using a novel functional screen. | lyophilized albumin protein fractions were prepared from flour of four varieties of wheat: triticum aestivum cvs. mercia and riband, triticum aestivum var. spelta, and triticum turgidum var. durum (kamut). the dry powders were redissolved in sodium phosphate buffers at ph 3.0, 6.5, or 8.0 and at ionic strengths of 0.1 or 1.0 m to a concentration of 0.1% (w/v). emulsions formed by sonication of protein solutions with n-hexadecane were aged at room temperature and separated into aqueous, interstit ... | 2003 | 12643668 |
assessment of the agronomic and technological characteristics of triticum turgidum ssp. dicoccum schrank and t. spelta l. | recently, the peoples interest in natural and organic products led to a "rediscovery" of hulled wheat, particularly t. turgidum ssp. dicoccum, commonly known as "farro". compared with durum wheat (t. turgidum ssp. durum), farro is characterized by low productivity and low quality semolina and flour, which makes it unsuitable for the production of bread and pasta. in this paper, the agronomic, technological and nutritional characteristics of three new varieties of farro obtained by crossing the t ... | 0 | 12653440 |
cadmium absorption and retention by rats fed durum wheat (triticum turgidum l. var. durum) grain. | a whole-body radioassay procedure was used to assess the retention and apparent absorption by rats of cd in kernels of durum wheat (triticum turgidum l. var. durum) harvested from plants grown hydroponically in 109cd-labelled nutrient solution. wholegrain wheat, containing 5 micromol cd (570 microg)/kg dry weight labelled intrinsically with 109cd, was incorporated into test meals fed to rats that had been maintained on diets containing marginally adequate, adequate or surplus levels of zn (0.12 ... | 2003 | 12654168 |
rapid genome divergence at orthologous low molecular weight glutenin loci of the a and am genomes of wheat. | to study genome evolution in wheat, we have sequenced and compared two large physical contigs of 285 and 142 kb covering orthologous low molecular weight (lmw) glutenin loci on chromosome 1as of a diploid wheat species (triticum monococcum subsp monococcum) and a tetraploid wheat species (triticum turgidum subsp durum). sequence conservation between the two species was restricted to small regions containing the orthologous lmw glutenin genes, whereas >90% of the compared sequences were not conse ... | 2003 | 12724543 |
accumulation of cadmium by durum wheat roots: bases for citrate-mediated exceptions to the free ion model. | the accumulation of cd in durum wheat (triticum turgidum) roots from hydroponic solutions, with the proportion of total cd (8.9-445 nm cd) as cd2+ varied by the addition of citrate, was determined to test the free-ion model (fim) of metal bioavailability for higher plants. calcium, mg, and k were also varied. citrate enhanced root-cd accumulation at higher cd2+ concentrations but not lower relative to the same cd2+ concentrations in solutions containing 0 mm citrate. elevating ca2+ and mg2+ conc ... | 2003 | 12729228 |
a new hessian fly resistance gene (h30) transferred from the wild grass aegilops triuncialis to hexaploid wheat. | a new hessian fly (mayetiola destructor say) resistance gene from aegilops triuncialis and its transfer to hexaploid wheat via interspecific hybridisation is described. the transfer line tr-3531 (42 chromosomes), derived from the cross [(triticum turgidum x ae. triuncialis) x triticum aestivum] and carrying the heterodera avenae resistance gene cre7, showed a high level of resistance to the m. destructor biotype prevailing in the sw of spain. a single dominant gene (h30) seems to determine the h ... | 2003 | 12748776 |
construction and characterization of a half million clone bac library of durum wheat ( triticum turgidum ssp. durum). | durum wheat ( triticum turgidum ssp. durum, 2 n = 4 x = 28, genomes ab) is an economically important cereal used as the raw material to make pasta and semolina. in this paper we present the construction and characterization of a bacterial artificial chromosome (bac) library of tetraploid durum wheat cv. langdon. this variety was selected because of the availability of substitution lines that facilitate the assignment of bacs to the a and b genome. the selected langdon line has a 30-cm segment of ... | 2003 | 12830387 |
microsatellite analysis reveals a progressive widening of the genetic basis in the elite durum wheat germplasm. | it has been argued that the level of genetic diversity in the modern durum wheat ( triticum turgidum l. var. durum) elite germplasm may have declined due to the high selection pressure applied in breeding programs. in this study, 58 accessions covering a wide spectrum of genetic diversity of the cultivated durum wheat gene pool were characterized with 70 microsatellite loci (or simple sequence repeats, ssrs). on average, ssrs detected 5.6 different allelic variants per locus, with a mean diversi ... | 2003 | 12845433 |
precise mapping of a locus affecting grain protein content in durum wheat. | grain protein content (gpc) is an important factor in pasta and breadmaking quality, and in human nutrition. it is also an important trait for wheat growers because premium prices are frequently paid for wheat with high gpc. a promising source for alleles to increase gpc was detected on chromosome 6b of triticum turgidum var. dicoccoides accession fa-15-3 (dic). two previous quantitative trait locus (qtl) studies found that the positive effect of dic-6b was associated to a single locus located b ... | 2003 | 12923624 |
phenotypic assessment and mapped markers for h31, a new wheat gene conferring resistance to hessian fly (diptera: cecidomyiidae). | a new source of resistance to the highly virulent and widespread biotype l of the hessian fly, mayetiola destructor (say), was identified in an accession of tetraploid durum wheat, triticum turgidum desf., and was introgressed into hexaploid common wheat, triticum aestivum l. genetic analysis and deletion mapping revealed that the common wheat line contained a single locus for resistance, h31, residing at the terminus of chromosome 5bs. h31 is the first hessian fly-resistance gene to be placed o ... | 2003 | 12928782 |
transgene integration and chromosome alterations in two transgenic lines of tritordeum. | plants from two transgenic lines of tritordeum (an amphiploid between triticum turgidum cv. durum and hordeumn chilense) have been analyzed by fluorescence in-situ hybridization (fish) to characterize the transgene integration sites and chromosome rearrangements. transgenic lines were transformed in two different events with the genes encoding for the high-molecular-weight glutenin subunits (hmw-gs), 1ax1 and/or 1dx5. three integration sites and four translocations were detected. all three integ ... | 2003 | 14516065 |
a genetic linkage map of the durum x triticum dicoccoides backcross population based on ssrs and aflp markers, and qtl analysis for milling traits. | durum wheat ( triticum turgidum l. var durum) is mainly produced and consumed in the mediterranean region; it is used to produce several specific end-products; such as local pasta, couscous and burghul. to study the genetics of grain-milling quality traits, chromosomal locations, and interaction with the environment, a genetic linkage map of durum was constructed and the quantitative trait loci qtls for the milling-related traits, test weight (tw) and thousand-kernel weight (tkw), were identifie ... | 2004 | 14676946 |
correlation between phytotoxicity on annual ryegirass (lolium rigidum) and production dynamics of allelochemicals within root exudates of an allelopathic wheat. | an improved allelopathic correlation between phytotoxicity measured in root growth bioassay upon annual ryegrass (lolium rigidum gaud.) and the concentrations of a selection of dynamically produced allelochemicals quantified in the root exudates of cv. khapli wheat (triticum turgidum ssp. durum (desf.) husn.) monitored during the first 15 days of wheat seedling growth in a sterile, agar-water medium, has been established. changes over the 15-day growth period in the quantities of five exuded ben ... | 2003 | 14682511 |
chromosomal location of genes for novel glutenin subunits and gliadins in wild emmer wheat (triticum turgidum l. var. dicoccoides). | the glutenin and gliadin proteins of wild emmer wheat, triticum turgidum l. var. dicoccoides, have potential for improvement of durum wheat ( t. turgidum l. var. durum) quality. the objective of this study was to determine the chromosomes controlling the high molecular weight (hmw) glutenin subunits and gliadin proteins present in three t. turgidum var. dicoccoides accessions (israel-a, pi-481521, and pi-478742), which were used as chromosome donors in langdon durum- t. turgidum var. dicoccoides ... | 2004 | 14727030 |
differences among auxin treatments on haploid production in durum wheat x maize crosses. | three doubled haploid lines of durum wheat [triticum turgidum ssp. durum (desf.) husn.] were crossed with maize (zea mays l.), and five hormone treatments were applied to test their effect on the production of caryopses, embryos and haploid plants. the auxin treatments consisted of 100 mg/l 2,4-dichlorophenoxyacetic acid (2,4-d), 5 mg/l or 50 mg/l dicamba and two combination mixtures of 95/5 mg/l and 50/50 mg/l 2,4-d plus dicamba, respectively. hormones were added to the culture medium of the de ... | 2004 | 15048585 |
molecular cytogenetic characterization of an alloplasmic durum wheat line with a portion of chromosome 1d of triticum aestivum carrying the scs(ae) gene. | triticum aestivum (2n = 6x = 42, aabbdd) with triticum longissimum (2n = 2x = 14; s1s1) cytoplasm ((lo) cytoplasm) has normal fertility and plant vigor. however, the nucleus of durum wheat (triticum turgidum (2n = 4x = 28, aabb)) is incompatible with the t. longissimum cytoplasm, producing non-viable progeny. this incompatibility is alleviated by scs(ae), a species cytoplasm-specific (scs) gene, on the long arm of chromosome 1d (1dl) of common wheat. the hemizygous (lo) durum scs(ae) line is mal ... | 2004 | 15060617 |
cadmium uptake and translocation in seedlings of near isogenic lines of durum wheat that differ in grain cadmium accumulation. | cadmium (cd) concentrations in durum wheat (triticum turgidum l. var durum) grain grown in north american prairie soils often exceed proposed international trade standards. to understand the physiological processes responsible for elevated cd accumulation in shoots and grain, cd uptake and translocation were studied in seedlings of a pair of near-isogenic durum wheat lines, high and low for cd accumulation in grain. | 2004 | 15084224 |
genomic organization of the complex alpha-gliadin gene loci in wheat. | to better understand the molecular evolution of the large alpha-gliadin gene family, a half-million bacterial artificial chromosome (bac) library clones from tetraploid durum wheat, triticum turgidum ssp. durum (2n = 4x = 28, genome ab), were screened for large genomic segments carrying the alpha-gliadin genes of the gli-2 loci on the group 6 homoeologous chromosomes. the resulting 220 positive bac clones--each containing between one and four copies of alpha-gliadin sequences--were fingerprinted ... | 2004 | 15103408 |
rapid genome evolution revealed by comparative sequence analysis of orthologous regions from four triticeae genomes. | bread wheat (triticum aestivum) is an allohexaploid species, consisting of three subgenomes (a, b, and d). to study the molecular evolution of these closely related genomes, we compared the sequence of a 307-kb physical contig covering the high molecular weight (hmw)-glutenin locus from the a genome of durum wheat (triticum turgidum, aabb) with the orthologous regions from the b genome of the same wheat and the d genome of the diploid wheat aegilops tauschii (anderson et al., 2003; kong et al., ... | 2004 | 15122014 |
dynamics of the evolution of orthologous and paralogous portions of a complex locus region in two genomes of allopolyploid wheat. | two overlapping bacterial artificial chromosome (bac) clones from the b genome of the tetraploid wheat triticum turgidum were identified, each of which contains one of the two high-molecular-weight (hmw) glutenin genes, comprising the complex glu-b1 locus. the complete sequence (285 506 bp of dna) of this chromosomal region was determined. the two paralogous x-type ( glu-1-1 ) and y-type ( glu-1-2 ) hmw-glutenin genes of the complex glu-b1 locus were found to be separated by ca. 168 000 bp inste ... | 2004 | 15159634 |
chemical, technological, and nutritional characteristics of two lines of "farro" (triticum turgidum ssp. dicoccum). | in recent years, the renewed interest for foods with a natural image has increased the demand for dry pasta produced from "hulled" wheat such as the triticum turgidum ssp. dicoccum, also known as "farro". in order to contribute to the general knowledge, two lines of farro were considered in this study. to have a comparison, an old cultivar of triticum turgidum ssp. durum (senatore cappelli) in addition to a commercial semolina were also examined. all semolina samples were used to produce pasta s ... | 2004 | 15285114 |
processing of wild cereal grains in the upper palaeolithic revealed by starch grain analysis. | barley (hordeum vulgare l.) and wheat (triticum monococcum l. and triticum turgidum l.) were among the principal 'founder crops' of southwest asian agriculture. two issues that were central to the cultural transition from foraging to food production are poorly understood. they are the dates at which human groups began to routinely exploit wild varieties of wheat and barley, and when foragers first utilized technologies to pound and grind the hard, fibrous seeds of these and other plants to turn ... | 2004 | 15295598 |
extension of the messapia x dicoccoides linkage map of triticum turgidum (l.) thell. | a set of recombinant inbred lines (ril) derived from a cross between the cultivar messapia of durum wheat (triticum turgidum var. durum) and the accession mg4343 of t. turgidum var. dicoccoides was analysed to increase the number of assigned markers and the resolution of the previously constructed genetic linkage map. an updated map of the durum wheat genome consisting of 458 loci was constructed. these loci include 261 restriction fragment length polymorphisms (rflps), 91 microsatellites (simpl ... | 2004 | 15332129 |
differential accumulation of cd in durum wheat cultivars: uptake and retranslocation as sources of variation. | durum wheat (triticum turgidum l. var. durum) accumulates cd from the soil depending on various factors. when grown in hydroponic solution containing cd (20 microg l(-1)), roots had higher tissue cd concentrations than shoots or heads. kyle (the higher grain-cd accumulating cultivar) had lower root-cd, and greater shoot-cd and head-cd concentrations than arcola (the lower grain-cd accumulating cultivar). these cultivar differences were greater at flowering and ripening than at tillering. much of ... | 2004 | 15361533 |
radiation hybrid mapping of the species cytoplasm-specific (scsae) gene in wheat. | radiation hybrid (rh) mapping is based on radiation-induced chromosome breakage and analysis of chromosome segment retention or loss using molecular markers. in durum wheat (triticum turgidum l., aabb), an alloplasmic durum line [(lo) durum] has been identified with chromosome 1d of t. aestivum l. (aabbdd) carrying the species cytoplasm-specific (scsae) gene. the chromosome 1d of this line segregates as a whole without recombination, precluding the use of conventional genome mapping. a radiation ... | 2004 | 15454553 |
pcr identification of durum wheat bac clones containing genes coding for carotenoid biosynthesis enzymes and their chromosome localization. | carotenoids are essential components in all plants. their accumulation in wheat seed determines the endosperm colour, which is an important quality trait in wheat. in this study, we report the isolation of bac clones containing genes coding for three different enzymes of the carotenoid biosynthesis pathway: phytoene synthase (psy), phytoene desaturase (pds), and zeta-carotene desaturase (zds). primers were designed on the basis of wheat ests similar to the sequences of these three genes in other ... | 2004 | 15499405 |
estimation of long-term effective population sizes through the history of durum wheat using microsatellite data. | estimation of long-term effective population size (n(e)) from polymorphism data alone requires an independent knowledge of mutation rate. microsatellites provide the opportunity to estimate n(e) because their high mutation rate can be estimated from observed mutations. we used this property to estimate n(e) in allotetraploid wheat triticum turgidum at four stages of its history since its domestication. we estimated the mutation rate of 30 microsatellite loci. allele-specific mutation rates mu we ... | 2005 | 15545658 |
ear of durum wheat under water stress: water relations and photosynthetic metabolism. | the photosynthetic characteristics of the ear and flag leaf of well-watered (ww) and water-stressed (ws) durum wheat (triticum turgidum l. var. durum) were studied in plants grown under greenhouse and mediterranean field conditions. gas exchange measurements simultaneously with modulated chlorophyll fluorescence were used to study the response of the ear and flag leaf to co2 and o2 during photosynthesis. c4 metabolism was identified by assessing the sensitivity of photosynthetic rate and electro ... | 2005 | 15645303 |
control of sodium transport in durum wheat. | in many species, salt sensitivity is associated with the accumulation of sodium (na(+)) in photosynthetic tissues. na(+) uptake to leaves involves a series of transport steps and so far very few candidate genes have been implicated in the control of these processes. in this study, na(+) transport was compared in two varieties of durum wheat (triticum turgidum) l. subsp. durum known to differ in salt tolerance and na(+) accumulation; the relatively salt tolerant landrace line 149 and the salt sen ... | 2005 | 15734907 |
chromosome sorting in tetraploid wheat and its potential for genome analysis. | this study evaluates the potential of flow cytometry for chromosome sorting in durum wheat (triticum turgidum desf. var. durum, 2n = 4x = 28). histograms of fluorescence intensity (flow karyotypes) obtained after the analysis of dapi-stained chromosomes consisted of three peaks. of these, one represented chromosome 3b, a small peak corresponded to chromosomes 1a and 6a, and a large peak represented the remaining 11 chromosomes. chromosomes sorted onto microscope slides were identified after fluo ... | 2005 | 15802508 |
genetic analysis of the species cytoplasm specific gene (scs d) derived from durum wheat. | the action of species cytoplasm specific (scs) gene(s) can be observed when a durum (triticum turgidum l.) nucleus is placed in the aegilops longissimum s. & m. cytoplasm. this alloplasmic combination, (lo) durum, results in nonviable progeny. a scs gene derived from t. timopheevii zhuk. (scs(ti)) produced compatibility with the (lo) cytoplasm. the resulting hemizygous (lo) scs(ti)- durum line was male sterile and when crossed to normal durum produced a 1:1 ratio of plump, viable (pv) seeds with ... | 2006 | 15843637 |
zinc effects on cadmium accumulation and partitioning in near-isogenic lines of durum wheat that differ in grain cadmium concentration. | here, we examined the effectiveness of two approaches for reducing cadmium (cd) accumulation in durum wheat (triticum turgidum l. var durum) grain: the application of supplemental zinc (zn), and the use of cultivars exhibiting reduced grain cd concentrations. two durum wheat near-isogenic lines (nil) that differ in grain cd accumulation were grown to maturity in solution culture containing a chelating agent to buffer the free activities of zn and cd at levels approximating those of field conditi ... | 2005 | 15998393 |
olive pomace amendment in mediterranean conditions: effect on soil and humic acid properties and wheat (triticum turgidum l.) yield. | the effects of the addition of either crude or exhausted olive pomace at two rates (10 and 20 t ha(-)(1)) on soil and soil humic acid (ha) properties and durum wheat (triticum turgidum l.) yield were investigated in open-field mediterranean conditions. soil amendment with olive pomaces produced a significant increase of total organic, total extractable, humified and nonhumified c forms, and available k contents. with respect to control soil ha, humic-like acids isolated from crude and exhausted ... | 2005 | 16104792 |
[chlorophyllian durum wheat plants obtained by isolated microspores culture: importance of the pre-treatments]. | in triticum turgidum subsp. durum (desf.) husn., the utilization of in vitro anther culture is hampered by the very high frequency of albinism of the regenerated plants reaching in most cases 100%. only in vitro ovary culture or intergeneric crosses with maize produce gynogenetic green haploid and doubled haploid plants. this paper is concerned with another very interesting method of androgenetic doubled haploid plant production, the in vitro isolated microspore culture. it is shown that this me ... | 2005 | 16125649 |
genetic characterization and molecular mapping of a hessian fly-resistance gene transferred from t. turgidum ssp. dicoccum to common wheat. | a gene (temporarily designated hdic) conferring resistance to the hessian fly (hf) [mayetiola destructor (say)] was previously identified from an accession of german cultivated emmer wheat [triticum turgidum ssp. dicoccum (schrank ex schübler) thell] pi 94641, and was transferred to the hf-resistant wheat germplasm ks99wgrc42. the inheritance of hdic resistance exhibited incomplete penetrance because phenotypes of some heterozygous progenies are fully resistant and the others are fully susceptib ... | 2005 | 16136351 |
molecular cytogenetic characterization and seed storage protein analysis of 1a/1d translocation lines of durum wheat. | two durum wheat [triticum turgidum l. ssp. durum (desf.) husn.] lines carrying the high-molecular-weight (hmw) glutenin subunits (gs) 1 d x 5 + 1dy10 encoded by glu-d1d, l252 and s99b34, were characterized using fluorescent genomic in-situ hybridization (fgish) and microsatellite markers. these two durum lines were derived from the crosses in which the common wheat (t. aestivum l.) 'len' and durum wheat 'langdon' (ldn) and 'renville' were involved. fgish patterns of the mitotic chromosomes indic ... | 2005 | 16170620 |
comparative analysis in cereals of a key proline catabolism gene. | proline accumulation and catabolism play significant roles in adaptation to a variety of plant stresses including osmotic stress, drought, temperature, freezing, uv irradiation, heavy metals and pathogen infection. in this study, the gene delta1 -pyrroline-5-carboxylate dehydrogenase (p5cdh), which catalyzes the second step in the conversion of proline to glutamate, is characterized in a number of cereal species. p5cdh genes from hexaploid wheat, triticum turgidum (durum wheat), aegilops tauschi ... | 2005 | 16179990 |
high-temperature adult-plant (htap) stripe rust resistance gene yr36 from triticum turgidum ssp. dicoccoides is closely linked to the grain protein content locus gpc-b1. | several new races of the stripe rust pathogen have become frequent throughout the wheat growing regions of the united states since 2000. these new races are virulent to most of the wheat seedling resistance genes limiting the resistance sources that can be used to combat this pathogen. high-temperature adult-plant (htap) stripe rust resistance has proven to be more durable than seedling resistance due to its non-race-specific nature, but its use is limited by the lack of mapping information. we ... | 2005 | 16208504 |
production of doubled haploids in durum wheat (triticum turgidum l.) through isolated microspore culture. | the objective of this work was to produce doubled haploid plants from durum wheat through the induction of androgenesis. a microspore culture technique was developed and used to produce fertile doubled haploid plants of agronomic interest. five cultivars, one selected line, plus a collection of 20 f(1) crosses between different genotypes of high breeding value were used. studies on several factors such as pre-treatments and media components were carried out in order to develop a protocol to rege ... | 2006 | 16220343 |
aluminium causes variable responses in actin filament cytoskeleton of the root tip cells of triticum turgidum. | the effects of aluminium on the actin filament (af) cytoskeleton of triticum turgidum meristematic root tip cells were examined. in short treatments (up to 2 h) with 50-1000 microm alcl3.6h2o, interphase cells displayed numerous afs arrayed in thick bundles that lined the plasmalemma and traversed the endoplasm in different directions. measurements using digital image analysis and assessment of the overall af fluorescence revealed that, in short treatments, the affected cells possessed 25-30% mo ... | 2005 | 16228895 |
map-based analysis of genes affecting the brittle rachis character in tetraploid wheat (triticum turgidum l.). | the mature spike rachis of wild emmer [triticum turgidum l. ssp. dicoccoides (körn. ex asch. and graebner) thell.] disarticulates spontaneously between each spikelet leading to the dispersion of wedge-type diaspores. by contrast, the spike rachis of domesticated emmer (triticum turgidum l. ssp. turgidum) fails to disarticulate and remains intact until it is harvested. this major distinguishing feature between wild and domesticated emmer is controlled by two major genes, brittle rachis 2 (br-a2) ... | 2006 | 16328232 |
a cytomolecular approach to assess the potential of gene transfer from a crop (triticum turgidum l.) to a wild relative (aegilops geniculata roth.). | when a crop hybridizes with a wild relative, the potential for stable transmission to the wild of any crop gene is directly related to the frequency of crop-wild homoeologous pairing for the chromosomal region where it is located within the crop genome. pairing pattern at metaphase i (mi) has been examined in durum wheat x aegilops geniculata interspecific hybrids (2n=4x=abugmg) by means of a genomic in-situ hybridization procedure that resulted in simultaneous discrimination of a, b and wild ge ... | 2006 | 16333611 |
physical map of the wheat high-grain protein content gene gpc-b1 and development of a high-throughput molecular marker. | grain protein content (gpc) is important for human nutrition and has a strong influence on pasta and bread quality. a quantitative trait locus, derived from a triticum turgidum ssp. dicoccoides accession (dic), with an average increase in gpc of 14 g kg(-1) was mapped on chromosome 6bs. using the wheat-rice colinearity, a high-density map of the wheat region was developed and the quantitative trait locus was mapped as a simple mendelian locus designated gpc-b1. a physical map of approx. 250 kb o ... | 2006 | 16441756 |
detection of qtls for grain protein content in durum wheat. | grain protein content (gpc) of durum wheat (triticum turgidum l. var. durum) is an important trait for the nutritional value of grain and for influencing the technological property of flour. protein content is a quantitative trait negatively correlated with grain yield, thus increase in protein quantity usually results in yield reduction. this study was initiated to introgress alleles for high gpc from var. dicoccoides into durum wheat germplasm by the backcross inbred line (bil) method and to i ... | 2006 | 16453131 |
phylogenetic relationships of triticum and aegilops and evidence for the origin of the a, b, and d genomes of common wheat (triticum aestivum). | common wheat (triticum aestivum) has for decades been a textbook example of the evolution of a major crop species by allopolyploidization. using a sophisticated extension of the pcr technique, we have successfully isolated two single-copy nuclear genes, dmc1 and ef-g, from each of the three genomes found in hexaploid wheat (ba(u)d) and from the two genomes of the tetraploid progenitor triticum turgidum (ba(u)). by subjecting these sequences to phylogenetic analysis together with sequences from r ... | 2006 | 16504543 |
use of wild relatives to improve salt tolerance in wheat. | there is considerable variability in salt tolerance amongst members of the triticeae, with the tribe even containing a number of halophytes. this is a review of what is known of the differences in salt tolerance of selected species in this tribe of grasses, and the potential to use wild species to improve salt tolerance in wheat. most investigators have concentrated on differences in ion accumulation in leaves, describing a desirable phenotype with low leaf na+ concentration and a high k+/na+ ra ... | 2006 | 16513812 |
role of cytoplasm-specific introgression in the evolution of the polyploid wheats. | studies of n-banded mitotic and meiotic karyotypes of triticum turgidum l. (2n = 28; aabb) and triticum timopheevii zhuk. (2n = 28; aagg) and hybrids between them, along with observations of meiotic pairing between telocentrics of the ab-genome chromosomes and their respective homologues and homeologues in t. timopheevii, showed that chromosome 4 (m4) of triticum monococcum l. is present (as 4a(t)) in t. timopheevii but is lacking in t. turgidum. neither 4a nor 4b pairs with 4a(t), but 4a pairs ... | 1987 | 16578821 |
wheat tetrameric inhibitors of insect alpha-amylases: alloploid heterosis at the molecular level. | tetrameric inhibitors of heterologous alpha-amylases have been characterized in allohexaploid wheat, triticum aestivum (genomes aabbdd), as well as in triticum turgidum (aabb) and triticum tauschii (dd). their subunits have been identified as the previously described cm proteins. single oligomeric species were observed in t. turgidum (subunits cm2, cm3a, and cm16) and in t. tauschii (cm1, cm3d, and cm17) by a two-dimensional electrophoretic method that does not dissociate the inhibitors in the f ... | 1989 | 16594035 |
the inhibitory effects of coumarin on the germination of durum wheat (triticum turgidum ssp. durum, cv. simeto) seeds. | the event chain leading to germination blockage in durum wheat (triticum turgidum ssp. durum desf.) seeds exposed to the allelochemical coumarin (2h-chromen-2-one) was studied. the physiological and biochemical aspects thought to be critical for a successful seed germination were measured. at concentrations above 200 microm: , coumarin inhibited seed germination in a concentration-dependent manner. inhibition occurred early during seed imbibition (phase i), was rapid, and irreversible. during ph ... | 2006 | 16598652 |
genetic diversity of triticum turgidum l. based on microsatellite markers. | using microsatellite (ssr) markers, the genetic diversity and genetic relationships among 48 triticum turgidum l. accessions, including 30 triticum turgidum l. ssp. turgidum, 7 triticum turgidum l. ssp. durum, 4 triticum turgidum l. ssp. carthlicum, 3 triticum turgidum l. ssp. paleocolchicum, 2 triticum turgidum l. ssp. turanicum and 2 triticum turgidum l. ssp. polonicum accessions, were investigated. a total of 97 alleles were detected on 16 ssr loci. at each locus, the number of alleles ranged ... | 2006 | 16649667 |
the k/na selectivity of a cation channel in the plasma membrane of root cells does not differ in salt-tolerant and salt-sensitive wheat species. | the characteristics of cation outward rectifier channels were studied in protoplasts from wheat root (triticum aestivum l. and triticum turgidum l.) cells using the patch clamp technique. the cation outward rectifier channels were voltage-dependent with a single channel conductance of 32 +/- 1 picosiemens in 100 millimolar kcl. whole-cell currents were dominated by the activity of the cation outward rectifiers. the time- and voltage-dependence of these currents was accounted for by the summed be ... | 1991 | 16668441 |
molecular characterization of a diagnostic dna marker for domesticated tetraploid wheat provides evidence for gene flow from wild tetraploid wheat to hexaploid wheat. | all forms of domesticated tetraploid wheat (triticum turgidum, genomes aabb) are nearly monomorphic for restriction fragment length polymorphism (rflp) haplotype a at the xpsr920 locus on chromosome 4a (xpsr920-a1a), and wild tetraploid wheat is monomorphic for haplotype b. the xpsr920-a1a/b dimorphism provides a molecular marker for domesticated and wild tetraploid wheat, respectively. hexaploid wheat (triticum aestivum, genomes aabbdd) is polymorphic for the 2 haplotypes. bacterial artificial ... | 2006 | 16675504 |
quantification of genetic relationships among a genomes of wheats. | the genetic relationships of a genomes of triticum urartu (au) and triticum monococcum (am) in polyploid wheats are explored and quantified by aflp fingerprinting. forty-one accessions of a-genome diploid wheats, 3 of ag-genome wheats, 19 of ab-genome wheats, 15 of abd-genome wheats, and 1 of the d-genome donor ae. tauschii have been analysed. based on 7 aflp primer combinations, 423 bands were identified as potentially a genome specific. the bands were reduced to 239 by eliminating those presen ... | 2006 | 16699549 |
discovery and mapping of wheat ph1 suppressors. | pairing between wheat (triticum turgidum and t. aestivum) homeologous chromosomes is prevented by the expression of the ph1 locus on the long arm of chromosome 5b. the genome of aegilops speltoides suppresses ph1 expression in wheat x ae. speltoides hybrids. suppressors with major effects were mapped as mendelian loci on the long arms of ae. speltoides chromosomes 3s and 7s. the chromosome 3s locus was designated su1-ph1 and the chromosome 7s locus was designated su2-ph1. a qtl with a minor effe ... | 2006 | 16702426 |
genetic characterization and molecular mapping of hessian fly resistance genes derived from aegilops tauschii in synthetic wheat. | two synthetic hexaploid wheat lines (xaegilotriticum spp., 2n = 6x = 42, genomes aabbdd), sw8 and sw34, developed from the crosses of the durum wheat cultivar langdon (triticum turgidum l. var. durum, 2n = 4x = 28, genomes aabb) with two aegilops tauschii cosson accessions (2n = 2x = 14, genome dd), were determined to carry hessian fly [mayetiola destructor (say)] resistance genes derived from the ae. tauschii parents. sw8 was resistant to the hessian fly biotype great plains (gp) and strain vh1 ... | 2006 | 16775694 |
antioxidant properties of wheat as affected by pearling. | the effects of pearling on the content of phenolics and antioxidant capacity of two canadian wheat classes, namely, canada western amber durum; triticum turgidum l. var. durum; cwad) and canada western red spring; triticum aestivum l.; cwrs) were examined. the antioxidant activity of wheat phenolics was evaluated using oxygen radical absorbance capacity (orac), inhibition of photochemiluminescence (pcl), rancimat method, inhibition of oxidation of low-density lipoprotein, and dna. the phenolic c ... | 0 | 16910705 |