Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| studies on optically active pesticides, i synthesis and herbicidal activity of d(+) and 1(-) methyl-2-chloro-3-(4-chlorophenyl)-propionate. | d(+) and 1(-) methyl-2-chloro-3-(4-chlorophenyl)-propionate (common name chlorfenprop-methyl; trade mark bidisin) were prepared and their herbicidal activities studied on avena fatua l. and avena sativa l. ("flämingskrone"). the 1(-) enantiomer was found to be twice as active as the racemate; the d(+) form almost inactive. | 2016 | 134566 |
| the pharmacology of avena sativa. | the pharmacology of avena sativa has been investigated in laboratory animals following a report that tincture of avena sativa reduced the craying for cigarettes in man. the tincture, evaporated to dryness, craving for cigarettes in man. the tincture, evaporated to dryness, re-constituted in an equal volume of water and administered by stomach tube or intraperitoneal injection, antagonized the antinociceptive effect of morphine in two separate test (hot-plate and tail flick). compared with animal ... | 1975 | 237083 |
| [modification of smoking behavior using long-distance methods]. | 906 persons willing to quit smoking were allocated at random to several groups. the results show that (1) an extract of avena sativa has no effect on quantity smoked; (2) distribution of the various parts of a smoking cessation program over several days was no more effective than distribution at once; (3) stopping at once was more effective than progressive reduction of cigarettes smoked. | 2015 | 605682 |
| extraction and purification of a substance with luteinizing hormone releasing activity from the leaves of avena sativa. | attempts were made to purify the lh-releasing substance extracted from the leaves of avena sativa by means of two-step chromatographic procedures using a weakly acidic ion-exchange resin (cg-50,type ii) and deae-sephadex a-25 (coarse) with successful results. for preliminary fractionation of such starting materials as dried leaves, fresh leaves, and acetone-extracted powder(crude extracts), 5% acetate-buffered active carbon proved to be more effective than starch zone electrophoresis. from its b ... | 1976 | 781913 |
| pharmacotherapy with avena sativa - a double blind study. | hundred non-hospitalized smokers with an average consumption of 20 cigarettes per day were treated with an alcoholic extract of avena sativa for disaccustoming. the study was double blind. by using query-sheets personal contact was excluded. the first result was a placebo-effect of 35% for disaccustoming of smokers and no statistically significant effect of the extract of avena sativa. the second result was a difference of disaccustoming between light and heavy smokers. it was shown, that the ra ... | 1976 | 794001 |
| inhibition of iaa-induced elongation in avena coleoptile segments by lead: a physiological and an electron microscopic study. | a high resolution growth measuring apparatus was used to demonstrate the inhibition of auxin-induced cell elongation in oat coleoptile segments (avena sativa l. var holden) by lead at concentrations ranging from 2 x 10-6 m to 2 x 10-3 m. the inhibition was immediate, having no measurable lag period. electron micrographs of lead-treated and control segments revealed that in the treated material, lead became localized as electron-dense granules in the cell walls and in vesicles associated with dic ... | 1976 | 1001018 |
| reversible redistribution of phytochrome within the cell upon conversion to its physiologically active form. | the intracellular localization of phytochrome was seen in dark-grown oat (avena sativa l., cv. garry) and rice (oryza sativa l., cv. unknown) shoots after various light treatments using an indirect peroxidase-antiperoxidase antibody labeling method. phytochrome is generally distributed throughout the cytoplasm in cells of tissue that had not been exposed to light prior to fixation. within, at most, 8 min after the onset of saturating red irradiation, phytochrome, now present in the far-red-absor ... | 1975 | 1093170 |
| the limit dextrinases from ungerminated oats (avena sativa l.) and ungerminated rice (oryza sativa l.). | the limit dextrinases from ungerminated oats and rice have been purified, and their substrate specificity compared with a bacterial isoamylase preparation. both cereal enzymes could hydrolyse (1 yields6)-alpha-d-glucosidic linkages in oligosaccharide alpha-dextrins, pullulan, amylopectin, and the beta-limit dextrins of amylopectin and glycogen. however, under comparable conditions, they were unable to attack glycogens. | 1975 | 1170015 |
| regulation of tabtoxin production by the lema gene in pseudomonas syringae. | pseudomonas syringae pv. coronafaciens, a pathogen of oats, was mutagenized with tn5 to generate mutants defective in tabtoxin production. from a screen of 3,400 kanamycin-resistant transconjugants, seven independent mutants that do not produce tabtoxin (tox-) were isolated. although the tn5 insertions within these seven mutants were linked, they were not located in the previously described tabtoxin biosynthetic region of p. syringae. instead, all of the insertions were within the p. syringae pv ... | 1992 | 1314808 |
| identification of the three major coeliac immunoreactive proteins and one alpha-amylase inhibitor from oat endosperm. | six chloroform/methanol-soluble proteins from oat endosperm (avena sativa) have been isolated and characterized by a purification procedure based on extraction with volatile solvents, followed by reversed-phase high performance liquid chromatography. three of these proteins, with an assessed molecular weight of 25,000, 27,000 and 32,000 da, respectively, have been identified by immunoblotting using coeliac sera, as the major coeliac serum iga-binding components of oat endosperm. the n-terminal a ... | 1992 | 1526282 |
| cloning and sequencing of protochlorophyllide reductase. | putative protochlorophyllide reductase cdna clones (252 and 113) were isolated from an etiolated-oat (avena sativa) cdna library. these were used to indirectly characterize a further clone, p127, isolated from a lambda-phage gt11 cdna library. the latter (1.15 kb in length) was sequenced, and the derived amino acid sequence was shown to be remarkably similar to that derived from chemical analysis of a cnbr-cleavage fragment of the purified reductase, p127 codes for more than 95% of the reductase ... | 1990 | 1689568 |
| characterization of acyllipid: sterol glucoside acyltransferase from oat (avena sativa l.) seedlings. | membranous fractions from leaves of oat seedlings readily convert cholesterol beta-d-glucoside into its 6'-o-acyl derivative using endogenous acyllipids as acyl sources. experiments with delipidated enzyme preparations showed that among acyllipids present in oat leaves digalactosyldiacylglycerols are evidently the best acyl donors in this reaction. beside of sterol glucosides, the enzyme can acylate beta-d-glucosides of several other steroids, although at very different rates. | 1991 | 1796705 |
| some properties of the h-atpase activity present in root plasmalemma of avena sativa l. two different enzymes or one enzyme with two atp sites? | the effects of mg2+, k+ and atp on a h-atpase activity from a native plasmalemma fraction of oat roots were explored at 20 degrees c and ph 6.5. in the presence of 3 mm atp and no k+, h-atpase activity vs. [mg2+] approached a monotonic activation but it became biphasic, with a decline above 3 mm mg2+, in the presence of 20 mm k+. mg2+ inhibition occurred also in k-free solutions when [atp] was lowered to 0.05 mm. also, an apparent monotonic h-atpase activation by [k+] at 3.0 mm atp was transform ... | 1991 | 1827349 |
| molecular cloning and sequencing of cdnas encoding the proteolipid subunit of the vacuolar h(+)-atpase from a higher plant. | to understand the molecular structure of the vacuolar h(+)-translocating atpase from plants, cdnas encoding the n,n'-dicyclohexylcarbodiimide-binding 16-kda proteolipid from oat (avena sativa l. var. lang) have been obtained. a synthetic oligonucleotide corresponding to a region of the bovine proteolipid cdna (mandel, m., moriyama, y., hulmes, j.d., pan, y.-c.e., nelson, h., and nelson, n. (1988) proc. natl. acad. sci. u.s.a. 85, 5521-5524) was used to screen an oat cdna library constructed in l ... | 1991 | 1831453 |
| expression of ap 3, 4 and 5 isophytochromes in etiolated oat seedlings (avena sativa l.). | type 1 phytochrome from etiolated oat seedlings was digested with v-8 protease. microsequencing of a 13 kda fragment yielded a sequence of 31 amino acids. the fragment starting with the alanine residue at position 427 of the entire phytochrome amino acid sequence revealed a heterogeneity (threonine, alanine and asparagine) at position 10. this demonstrates that the phytochrome type a genes ap3, 4 and 5 are expressed as proteins. | 1991 | 1881964 |
| heterologous encapsidation in mixed infections among four isolates of barley yellow dwarf virus. | we used immunohybridization and elisa to investigate heterologous encapsidation (transcapsidation and phenotypic mixing) between paired isolates of barley yellow dwarf virus (bydv) in doubly infected oat plants, avena sativa l. cv. clintland 64. virons in samples extracted from plants doubly infected with two viruses were trapped with an antibody specific to one virus, and the nucleic acids of the trapped virions were identified with a cdna probe specific to the other. heterologous encapsidation ... | 1991 | 1895059 |
| intracellular protein phosphorylation in oat (avena sativa l.) protoplasts by phytochrome action: involvement of protein kinase c. | phosphorylations of two proteins (27 kda, 32 kda) in oat cells were dependent on phytochrome action. to determine which kinase system(s) for the phosphorylation of these two proteins are controlled by the phytochrome, involvement of the ca2+/dg dependent protein kinase (protein kinase c) was first investigated. when a protein kinase c inhibitor (1-(5-isoquinoline sulfonyl)-2-methylpiperazine:h-7) or the inositol phospholipid metabolic blocker li+ was added into the cell suspension, respectively, ... | 1990 | 2163631 |
| computer analysis of phytochrome sequences from five species: implications for the mechanism of action. | the amino acid sequences of phytochrome from avena sativa, oryza sativa, curcurbita pepo, pisum sativum and arabidopsis thaliana have been analyzed with a variety of computer programs, with a view to identifying areas of the protein which contribute to the properties of this photoreceptor. a region at the c-terminus has been shown to be amphiphilic, and by analogy with surface-seeking peptides, may be responsible for interaction of phytochrome with lipid bilayers. possible targeting sequences in ... | 2009 | 2291773 |
| genomic sequence of a 12s seed storage protein gene from oat (avena sativa l. cv. 'solidor'). | 1990 | 2326176 | |
| oscillations of acetylcholine in oat seedlings. | using gas chromatography it was shown that acetylcholine (ach) was present in both etiolated and green oat (avena sativa l. cv. diadem) seedlings. in etiolated seedlings the ach level was low, but increased rapidly during exposure to sunlight and red light (rl). the stimulative influence of rl was reversed by far-red light (frl). the rl- and frl- changes in ach level were correlated to changes in acetylcholinesterase (ache) localization. using karnovsky's method, it was found that in the etiolat ... | 2013 | 2350987 |
| co-ordination of chromophore-apoprotein synthesis in the developing leaf of avena sativa l. | 1990 | 2373249 | |
| [comparison of presence of ascorbic acid and the appearance of ascorbate peroxidase activity in embryos of avena sativa l]. | avena sativa l. grains are devoid of ascorbic acid (aa) and of oxidative enzymes (aa oxidase and aa peroxidase), while both reducing enzymes (afr reductase and dha reductase) are present. aa biosynthesis in the embryos starts after 12-14 hours of germination and at the same time aa peroxidase activity is detectable. during the following 14 hours the aa peroxidase activity rises up to 28 nmoles/aa oxidated/min/mg/prot. incubation of avena embryos with gl (the last precursor of aa according to the ... | 1990 | 2390226 |
| rapid transcriptional regulation by phytochrome of the genes for phytochrome and chlorophyll a/b-binding protein in avena sativa. | we have examined phytochrome-regulated transcription of phytochrome (phy) and chlorophyll a/b binding protein (cab) genes in dark-grown avena seedlings by using run-on transcription in isolated nuclei. kinetic analysis of phy transcription following pulse-light treatments to produce various amounts of pfr, the active form of phytochrome, leads to these conclusions. (i) transcription decreases rapidly (discernible within 5 min) after pfr formation, reaching an essentially undetectable level by 1 ... | 1988 | 2463467 |
| mapping of antigenic domains on phytochrome from etiolated avena sativa l. by immunoblot analysis of proteolytically derived peptides. | several monoclonal antibodies to phytochrome that interact with putative functionally important domains have been previously identified. the locations of some of these domains are determined here by epitope mapping experiments that utilize immunoblot analyses of proteolytically degraded phytochrome. seven independent epitopes are identified. an epitope that is recognized by monoclonal antibody oat-25 is confirmed to be wholly located near the n terminus of phytochrome. this domain undergoes a co ... | 1988 | 2463784 |
| analysis of avenin proteins and the expression of their mrnas in developing oat seeds. | we have isolated and characterized cdna clones encoding avenins, the prolamine storage proteins of oat seeds. sequence analysis shows that avenins are a related group of polypeptides and that their mrnas differ from each other by point mutations and small insertions and deletions. avenin proteins have structural homology to the alpha/beta-gliadins and gamma-gliadins of wheat, the b-hordeins of barley, and the gamma-secalins of rye. hybridization analysis of dna from various diploid, tetraploid, ... | 1989 | 2535531 |
| glycolytic activity in embryos of pisum sativum and of non-dormant or dormant seeds of avena sativa l. expressed through activities of pfk and ppi-pfk. | a quantitative cytochemical assay for ppi-pfk activity in the presence of fru-2,6-p2 is described along with its application to determine levels of activity in embryos of pisum sativum and avena sativa. the activity of atp-pfk has also been studied in parallel as have pfk activities during the switch from dormant to non-dormant embryos in avena sativa. ppi-pfk activity has been demonstrated in all tissues of pisum sativum embryos and of avena sativa embryos including the scutellum and the aleuro ... | 1989 | 2541117 |
| unusual hepatic parenchymal crystalloid material and biliary microliths in goats. | investigation into an outbreak of suspected photosensitivity in boer goats grazing green oats (avena sativa) led to the finding of microscopical parenchymal crystaloid material and biliary microliths in the livers of 3 ewes that were killed for necropsy. neither tribulus terrestris nor panicum spp. occurred on the farm. further investigation resulted in the isolation, from leaf spots on the oat leaves, of the fungus drechslera campanulata, cultures of which have been shown to be highly toxic to ... | 1989 | 2634769 |
| domain structure of phytochrome from avena sativa visualized by electron microscopy. | highly purified phytochrome from avena sativa was visualized by electron microscopy after negative staining with uranyl acetate and after rotary shadowing with platinum. the particle shape was variable in both types of specimens, but tripartite structures resembling a 'y' were consistently observed. the tripartite substructure is composed of three globular domains each having a diameter of 7 to 8 nm and equally spaced in an equilateral triangle. the dimensions of the tripartite particle measured ... | 1989 | 2727087 |
| intracellular protein phosphorylation in oat (avena sativa l.) protoplasts by phytochrome action. 1. measurement of action spectra for the protein phosphorylation. | the effects of red light and wavelength dependency of the protein phosphorylation in oat protoplasts were investigated by sodium dodecyl sulfate polyacrylamide gel electrophoresis and autoradiography. red light (660 nm) irradiation of the protoplasts increased the phosphorylation of 15 different proteins, and the phosphorylation of 2 proteins (27 kda, 32 kda) out of 15 were observed to be dependent on the wavelength of the irradiating light. the phosphorylation densities of these two proteins in ... | 1989 | 2751674 |
| hemin inhibits ubiquitin-dependent proteolysis in both a higher plant and yeast. | in eukaryotes, a major route for atp-dependent protein breakdown proceeds through covalent intermediates of target proteins destined for degradation and the highly conserved, 76 amino acid protein ubiquitin. in rabbit reticulocytes, it has been shown that hemin effectively inhibits this pathway by blocking the catabolism of ubiquitin-protein conjugates [ki = 25 microm (haas, a. l., & rose, i. a. (1981) proc. natl. acad. sci. u.s.a. 78, 6845-6848)]. here, we demonstrate that hemin is also an effe ... | 1988 | 2839230 |
| probing the catalytic subunit of the tonoplast h+-atpase from oat roots. binding of 7-chloro-4-nitrobenzo-2-oxa-1,3,-diazole to the 72-kilodalton polypeptide. | the purified tonoplast h+-atpase from oat roots (avena sativa l. var. lang) consists of at least three different polypeptides with masses 72, 60, and 16 kda. we have used covalent modifiers (inhibitors) and polyclonal antibodies to identify the catalytic subunit of the h+-pumping atpase. the inactivation of atpase activity by 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (nbd-cl, an adenine analog) was protected by mgatp or mgadp, and showed kinetic properties consistent with active site-directed inhi ... | 1987 | 2884218 |
| n,n'-dicyclohexylcarbodiimide-binding proteolipid of the vacuolar h+-atpase from oat roots. | the inhibitor n,n'-dicyclohexylcarbodiimide (dccd) was used to probe the structure and function of the vacuolar h+-translocating atpase from oat roots (avena sativa var. lang). the second-order rate constant for dccd inhibition was inversely related to the concentration of membrane, indicating that dccd reached the inhibitory site by concentrating in the hydrophobic environment. [14c]dccd preferentially labeled a 16-kda polypeptide of tonoplast vesicles, and the amount of [14c]dccd bound to the ... | 1988 | 2891708 |
| peripheral and integral subunits of the tonoplast h+-atpase from oat roots. | the subunit organization of the tonoplast h+-pumping atpase from oat roots (avena sativa l. var. lang) was investigated. tonoplast vesicles were treated with low ionic strength solutions (0.1 mm 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid buffer or 0.1 mm na edta), carbonate, or a chaotropic reagent (ki), and then centrifuged to give a soluble fraction and a pellet. treatments with low ionic strength solutions or ki resulted in 70-80% reduction in the membrane-associated atpase activity, ... | 1988 | 2903155 |
| h+-atpase from plasma membranes of saccharomyces cerevisiae and avena sativa roots: purification and reconstitution. | 1988 | 2906717 | |
| purification and initial characterization of ubiquitin from the higher plant, avena sativa. | ubiquitin is a highly conserved, 76-amino acid polypeptide recently demonstrated to be involved in atp-dependent protein degradation in mammalian cells. from immunoblot analyses with anti-human-ubiquitin antibodies we have detected the presence of free ubiquitin in green leaves, etiolated shoots, and dry seeds of the higher plant, oats (avena sativa l.). we also find that crude oat extracts contain protease(s) that rapidly degrade both oat and human ubiquitin (t1/2 approximately 10 min at 27 deg ... | 1985 | 2995356 |
| [primary structure of the "fast" component of avenin (avena sativa l]. | a procedure for the isolation and sequence analysis of the "fast" avenin component (n9) from the oat (avena sativa l., cv. narymsky 943) is described. component n9 was prepared by an ion-exchange high-performance liquid chromatography on a strong cation exchange column type mono s (pharmacia, sweden) in 4 m urea, ph 3.5, with a linear gradient of nacl. a polypeptide chain of avenin n9 was reconstructed by the cnbr and tryptic peptides on a model 470a protein gas-phase sequencer (applied biosyste ... | 2011 | 3190779 |
| agrobacterium-mediated infectivity of cloned digitaria streak virus dna. | a monomeric clone of double-stranded dna synthesized in vitro dna of the geminivirus digitaria streak (dsv) was subcloned as a tandem dimeric unit into a binary vector of agrobacterium tumefaciens, creating a plasmid pds2. inoculation of digitaria sanguinalis with a. tumefaciens carrying pds2 resulted in viral infection. the symptoms, virus particles, and dna forms obtained were indistinguishable from those of a natural dsv infection of d. sanguinalis. inoculations have also induced infections i ... | 1988 | 3341112 |
| uptake of 226ra by established vegetation and black cutworm larvae, agrotis ipsilon (class insecta: order lepidoptera), on u mill tailings at elliot lake, canada. | radium-226 levels in samples from an inactive u tailings site at elliot lake, ontario, canada, were: 9,140 +/- 500 mbq g-1 dry weight in the substrate; 62 +/- 1 mbq g-1 dry weight in rye, secale cereale, and less than 3.7 mbq g-1 dry weight in oats, avena sativa, the dominant species established by revegetation of the tailings; and 117 +/- 7 mbq g-1 dry weight in washed and unwashed black cutworm larvae. concentration ratios were: vegetation to tailings 0.001-0.007; black cutworms to vegetation ... | 1988 | 3391775 |
| comparison of the protein conformations between different forms (pr and pfr) of native (124 kda) and degraded (118/114 kda) phytochromes from avena sativa. | 1987 | 3562595 | |
| homology between legumin-like polypeptides from cereals and pea. | the presence of legumin-like constituents within the globulin fractions of wheat (triticum aestivum), rye (secale cereale) and corn (maize, zea mays) was demonstrated. two-dimensional analysis of wheat globulins in the presence and absence of a reducing agent revealed the existence of reducible approximately 60 kda polypeptides. western-blot analysis with 125i-labelled antibodies raised against the oat (avena sativa) 12s globulin holoprotein or its alpha-subunits demonstrated, firstly, the immun ... | 1985 | 3985947 |
| an experimental mycotoxicosis in sheep and goats caused by drechslera campanulata, a fungal pathogen of green oats. | field outbreaks of a syndrome of unknown aetiology associated with the grazing of green oats (avena sativa) in the south-western cape province were characterized by diarrhoea, photosensitivity and death in goats and by diarrhoea and a reduction in milk production in cows. a phytopathogenic fungus, drechslera campanulata, was isolated from conspicuous reddish-brown leaf spots on oat plants collected from both outbreaks. pure cultures on autoclaved maize of d. campanulata isolates from oat leaves ... | 1985 | 4047622 |
| [experiments on the localisation of flavonoids in plastids. i. flavonoids in etioplasts of avena sativa l]. | 1972 | 4405256 | |
| lack of effect of avena sativa on cigarette smoking. | 1974 | 4431518 | |
| proceedings: on the pharmacology of an extract of avena sativa. | 1974 | 4451796 | |
| letter: treatment of nicotine addiction with avena sativa. | 1974 | 4471694 | |
| somatic association of chromosomes in asynaptic genotypes of avena sativa l. | 1973 | 4712858 | |
| production of aneuploids in avena sativa l. | 1973 | 4762793 | |
| effect of avena sativa on cigarette smoking. | 1971 | 4939551 | |
| [localization of calcium at the level of chloroplasts of oat leaf (avena sativa l) by a very high resolution trace-autoradiography method]. | 1969 | 4989893 | |
| studies on phytochrome. some properties of electrophoretically pure phytochrome. | 1. phytochrome was purified from etiolated oat (avena sativa) seedlings either by gel-filtration chromatography and ion-exchange chromatography or by gel-filtration chromatography and calcium phosphate chromatography. differences were observed in the spectral properties of phytochrome isolated by the two methods. 2. electrophoresis of pure phytochrome at ph values between 9.0 and 6.0 showed the tendency of phytochrome to form different molecular species. studies in the ultracentrifuge did not sh ... | 1970 | 5499974 |
| colorimetric determination of aconitic acid in avena sativa (oat). | 1970 | 5530564 | |
| electrophoretic variation in esterases of three varieties of oats (avena sativa). | 1968 | 5723612 | |
| cross protection and mutual exclusion by three strains of barley yellow dwarf virus in avena sativa l. | 1965 | 5833319 | |
| movement of indoleacetic acid in coleoptiles of avena sativa l. ii. suspension of polarity by total inhibition of the basipetal transport. | acropetal and basipetal movement of indole-3-acetic acid through coleoptiles of avena sativa l. was studied. sections 10-mm long were supplied with either apical or basal sources containing c(14) carboxyl-labeled indoleacetic acid (10(-5)m). anaerobic conditions inhibit metabolically dependent movement (transport) thus reducing basipetal but not acropetal movement. total inhibition of basipetal transport abolishes the polarity of auxin uptake and movement. the nonpolar movement that remains in a ... | 1966 | 5904589 |
| the effects of some auxins on the levels of phosphate esters in avena sativa coleoptile sections. | 1967 | 6049497 | |
| metabolic turnover in cell wall constituents of avena sativa l. coleoptile sections. | 1967 | 6051572 | |
| a cell wall polysaccharide-hydrolyzing enzyme system in avena sativa l. coleoptiles. | 1967 | 6051573 | |
| glycerate kinase from leaves of c3 plants. | d-glycerate-3-kinase (ec 2.7.1.31) in six c3 species, including dicots (pisum sativum, spinacea oleracea, antirrhinum majus) and monocots (secale cereale, hordeum vulgare, avena sativa), ranged in activity from 44 to 353 mumol x mg chl-1 x h-1. studies with protoplast extracts of these species indicate that the enzyme is localized in the chloroplasts. glycerate kinase was partially purified from secale (rye, 288-fold) and pisum (pea, 252-fold) chloroplasts by deae-cellulose chromatography, sucro ... | 1983 | 6307152 |
| cell fusion by simulated atmospheric discharges: further support for the hypothesis of involvement of electrofusion in evolution. | electrofusion of mesophyll cell protoplasts of avena sativa was induced by simulated atmospheric discharges. it is shown both experimentally and theoretically that the potential differences which occur at quite long distances from the point of lightning stroke are large enough to induce fusion. besides electromagnetic waves which are emitted during lightning (g. küppers and u. zimmermann, febs lett. 164, 323 (1983] cell fusion may have also occurred directly by means of the voltage built-up on t ... | 2016 | 6516538 |
| a report on the consumption, composition and nutritional adequacy of a mixture of lush green perennial ryegrass (lolium perenne) and cocksfoot (dactylis glomerata) fed ad libitum to thoroughbred mares. | non-pregnant thoroughbred mares were stabled and subjected to 2 trials, each 24 h in duration, to establish their total consumption of a mixture of freshly cut, lush green perennial ryegrass (lolium perenne) and cocksfoot (dactylis glomerata) in approximately equal proportions; and to compare the total intake of crude protein, calcium, phosphorus and mass of the grass mixture on a dry matter basis with their daily nutritional requirements. the body mass of each mare was calculated at the commenc ... | 1983 | 6655653 |
| stereospecific analysis of triglycerides of glycine max, glycine soya, avena sativa and avena sterilis strains. | a rapid method for the stereospecific analysis of triglycerides based on enzymatic hydrolysis on thin layer plates was applied to a number of glycine max, glycine soya, avena sativa and avena sterilis strains. the percentage of each fatty acid on the sn-1-, sn-2- and sn-3-positions was linearly related to the total percentage of the fatty acid in the triglyceride. large deviations from the common triglyceride pattern were not found. | 1983 | 6686643 |
| purification, characterization, and kinetic mechanism of s-adenosyl-l-methionine: vitexin 2"-o-rhamnoside 7-o-methyltransferase of avena sativa l. | an o-methyltransferase catalyzing the transfer of the methyl group of s-adenosyl-l-methionine to the a-ring 7-hydroxyl group of vitexin 2"-o-rhamnoside has been isolated from oat primary leaves and purified 180-fold by protein fractionation with (nh4)2so4 and chromatography on deae-cellulose and s-adenosyl-l-homocysteine-sepharose. km values for s-adenosyl-l-methionine and the flavonoid substrate were 1.6 microm and 15 microm, respectively. the lack of methyltransfer to biosynthetic intermediate ... | 1984 | 6705789 |
| rotation of cells and ion exchange beads in the mhz-frequency range. | whereas human red blood cells and protoplasts of leaves of avena sativa show rotation only at discrete frequencies in the khz-range of a linear alternating electric field, rotation of these cells is observed at practically every frequency in the mhz range (15 to 200 mhz). the cell rotation in the mhz-range can be explained in terms of field induced polarisation and orientation of permanent dipoles within the membrane and the cell. this interpretation is supported by the finding that chelex beads ... | 1983 | 6880341 |
| structure-function studies on phytochrome. preliminary characterization of highly purified phytochrome from avena sativa enriched in the 124-kilodalton species. | a new protocol for the purification of phytochrome from etiolated avena sativa l. seedlings in 15-25% yield is described. this preparation exhibits molecular properties dissimilar to those previously reported from oats. phytochrome prepared by this method contains a dominant species having a mr = 124,000 (70% of total) on sodium dodecyl sulfate-polyacrylamide gels, in addition to a chromoprotein species with mr = 118,000 (30%). a blocked nh2 terminus is indicated by amino acid sequencing of the ... | 1983 | 6885811 |
| rotation of cells in an alternating electric field: the occurrence of a resonance frequency. | cells suspended in a low-conducting medium were exposed to an alternating electric field whose frequency was altered between 1 khz and 2 mhz. a resonance frequency was observed at which all suspended cells rotated about an axis normal to the field lines (when the electric field strength was larger than a threshold value of about 400 v/cm). this resonance frequency varied from species to species of cells (mesophyll protoplasts of avena sativa = 20-40 khz, human erythrocytes and ghost cells = 80-1 ... | 1981 | 7010816 |
| rotation of cells in an alternating electric field: theory and experimental proof. | protoplasts of avena sativa rotate in an alternating electric field provided that at least two cells are located close to each other. an optimum frequency range (20 to 30 khz) exists where rotation of all cells exposed to the field is observed. below and above this frequency range, rotation of some cells is only occasionally observed. the angular velocity of rotation depends on the square of the electric field strength. at field strengths above the value leading to electrical breakdown of the ce ... | 1982 | 7097755 |
| physicochemical differences between the red- and the far-red-absorbing forms of phytochrome. | we investigated some of the chemical and physical differences between the red-absorbing (pr) and far-red-absorbing (pfr) forms of immunoaffinity-purified, undegraded oat (avena sativa l., ev. garry) phytochrome. both pr and pfr had identical surface charges as measured by isoelectric focusing and identical secondary structure as judged from their circular dichroic spectra. modification of specific amino acid residues, however, revealed some chemical differences between pr and pfr. compared to pr ... | 1981 | 7213624 |
| fusion of avena sativa mesophyll cell protoplasts by electrical breakdown. | studies with the light microscope were carried out on mesophyll cell protoplasts of avena sativa which had been made to undergo fusion by reversible electrical breakdown of the cell membrane. in order to establish close membrane contact between the cells, an important prerequisite for fusion, a method known as dielectrophoresis was used. in an inhomogeneous alternating electrical field the protoplasts adhere to the electrodes and to each other in the direction of the field lines. the cells which ... | 1981 | 7213711 |
| use of lilium longiflorum, cv. ace pollen germination and tube elongation as a bioassay for the hepatocarcinogens, aflatoxins. | although various animal tissues are used for bioassay of aflatoxins (b1, b2, g1, g2), a rapid bioassay dependent upon a plant part's response does not exist. both pollen germination (g) and tube elongation (te) were enhanced in a 3.0 mm kh2po4 (k)-containing but afb1-lacking, modified dickinson's medium. the b1 did not affect g when k was withheld but k supplementation impaired g above 15 micrograms/ml b1. without k, 5-20 stimulated but 25 and 30 micrograms/ml b1 inhibited te which was suppresse ... | 1981 | 7274186 |
| covalent labelling of the nadph: protochlorophyllide oxidoreductase from etioplast membranes with [3h]n-phenylmaleimide. | [3h]n-phenylmaleimide has been used to covalently label in a specific manner the substrate-protected thiol groups of the enzyme protochlorophyllide reductase. in membrane preparations from oat (avena sativa) and runner-bean (phaseolus vulgaris) seedlings, two related peptides of mol.wts. 35000/37000 and 34000/35000 respectively and showing properties expected of the reductase have been identified, whereas the same technique with barley (hordeum vulgare) extracts resulted in labelling a single pe ... | 1981 | 7306063 |
| partial characterization of undegraded oat phytochrome. | we characterized immunoaffinity-purified, undegraded oat (avena sativa l., cv. garry) phytochrome by several physicochemical techniques. phytochrome, of greater than 98% purity [hunt, r. e., & pratt, l. h. (1979) plant physiol. 64, 332-336], existed in solution as a dimer of its 118 000-dalton monomers and had a full complement of the typical amino acids with about 35% nonpolar residues, 115 carboxylic acid groups per monomer, and an average of one phosphate per monomer. although the dimer was n ... | 1980 | 7352993 |
| detection and partial characterization of activity of chlorophyll synthetase in etioplast membranes. | the esterification of chlorophyllide a was investigated an irradiated etioplast-membrane fractions ('broken etioplasts') from oat seedlings (avena sativa l.). as a substrate, [1(-3)h]geranylgeraniol and its monophosphate and diphosphate derivatives were prepared by chemical synthesis. geranylgeraniol and its monphosphate derivative are incorporated into chlorophyll only in the presence of atp whereas the diphosphate derivative is incorporated also without atp. the yield of esterified chlorophyll ... | 1980 | 7408876 |
| levels of a terpenoid glycoside (blumenin) and cell wall-bound phenolics in some cereal mycorrhizas. | four cereals, hordeum vulgare (barley), triticum aestivum (wheat), secale cereal (rye), and avena sativa (oat), were grown in a defined nutritional medium with and without the arbuscular mycorrhizal fungus glomus intraradices. levels of soluble and cell wall-bound secondary metabolites in the roots of mycorrhizal and nonmycorrhizal plants were determined by high-performance liquid chromatography during the first 6 to 8 weeks of plant development. whereas there was no difference in the levels of ... | 1995 | 7480342 |
| comparative mapping in grasses. oat relationships. | the development of rflp linkage maps in hexaploid and diploid oat allows us to study genetic relationships of these species at the dna level. in this report, we present the extension of a previously developed diploid oat map (avena atlantica x a. hirtula) and its molecular-genetic relationships with wheat, rice and maize. examination of 92-99% of the length of the oat genome map with probes common to triticeae species, rice or maize showed that 84, 79 and 71%, respectively, was conserved between ... | 1995 | 7500960 |
| pollen allergen homologues in barley and other crop species. | pollen from 10 agricultural plant species was surveyed for the presence of proteins crossreactive with group i, group iv and group ix allergens. barley (hordeum vulgare), maize (zea mays), rye (secale cerale), triticale (xtriticosecale cereale), oats (avena sativa), canola (brassica napus) and sunflower (helianthus annus) pollens contained numerous allergen cognate proteins. northern blot analysis of barley pollen rna revealed the presence of group i and group ix allergen transcripts. the barley ... | 1995 | 7537171 |
| fourier-transform resonance raman spectroscopy of intermediates of the phytochrome photocycle. | the parent states of the 124-kda phytochrome (phy a from avena sativa) and intermediates of its photocycle were studied by low-temperature fourier-transform resonance raman spectroscopy. spectra of the primary photoproducts i700 and lumi-f and of the thermal intermediate meta-f have been obtained for the first time. the spectra of the stable photochromic forms of photochrome, pr and pfr, presented in this work are significantly better in signal-to-noise ratio and resolution than previously publi ... | 1995 | 7654704 |
| genomic clones encoding 11s globulins in oats (avena sativa l.). | we have isolated two complete genomic clones, glav1 and glav3, encoding 11s globulins (legumins) in oat. the structure of glav1 deviates from that of the typical legumin gene. this clone possesses an extra intron and an extra exon that is composed entirely of repeats of sequences found elsewhere in the clone. if this exon is functional, the protein encoded by glav1 will contain novel octapeptide and hendecapeptide repeats. the two glav clones show stronger and more extensive homology with one an ... | 1995 | 7672598 |
| gibberellin-regulated expression in oat aleurone cells of two kinases that show homology to map kinase and a ribosomal protein kinase. | cdna fragments from ten different protein kinases expressed in avena sativa aleurone cells were amplified from mrna by rt-pcr with degenerate primers. these could be classified into five groups: aspk1-3 showed homology to the snf1-related protein kinases, aspk4-5 to a wheat aba up-regulated protein kinase, aspk6-8 to the ca-dependent, calmodulin-independent protein kinase family, aspk9 encoded a map kinase and aspk10 was closely related to a novel arabidopsis ribosomal protein kinase. ga caused ... | 1995 | 7766874 |
| two tcp-1-related but highly divergent gene families exist in oat encoding proteins of assumed chaperone function. | tcp-1-related sequences have been isolated from a cdna library of etiolated 6-day-old oat (avena sativa) seedlings. this attempt was made to obtain cdnas of a recently published 60 kda plant chaperone that re-folds denatured phytochrome and which was biochemically characterised as a tcp-1-related protein [(1993) nature 363, 644-647]. the translation of the putative coding sequence from one full-length cdna clone displays no specific homologies to amino acid sequences known from peptide sequencin ... | 1993 | 7903257 |
| the complete amino acid sequence and disulphide bond arrangement of oat alcohol-soluble avenin-3. | we have elucidated the complete amino acid sequence of one of the avenin components, avenin-3, isolated from oat (avena sativa l.), variety narymsky 943. the sequence of the protein was determined by sequencing of cnbr and trypsin-generated peptides in combination with mass spectrometry. the protein is a single polypeptide chain, consisting of 201 amino acid residues with m(r) 23,252.8. the n-terminal amino acid residue of the protein is blocked with 5-oxoproline (pyroglutamic acid). all eight c ... | 1994 | 7925380 |
| avenacosidase from oat: purification, sequence analysis and biochemical characterization of a new member of the bga family of beta-glucosidases. | a protein consisting of 60 kda subunits (as-p60) was isolated from etiolated oat seedlings (avena sativa l.) and characterized as avenacosidase, a beta-glucosidase that belongs to a preformed defence system of oat against fungal infection. the enzyme is highly aggregated; it consists of 300-350 kda aggregates and multimers thereof. dissociation by freezing/thawing leads to complete loss of enzyme activity. the specificity of the enzyme was investigated with para-nitrophenyl derivatives which ser ... | 1994 | 8000004 |
| the amino acid sequence previously attributed to a protein kinase or a tcp1-related molecular chaperone and co-purified with phytochrome is a beta-glucosidase. | a 60 kda protein (p60) co-purified with phytochrome was identified as avenacosidase, a beta-glucosidase which is part of the defense system of avena sativa. an antiserum raised against p60 was used to isolate a cdna clone coding for the complete amino acid sequence of p60. the cdna-derived amino acid sequence contained the partial sequences described before for a protein kinase [(1989) planta 178, 199-206] and for a tcp1-related molecular chaperone [(1993) nature 363, 644-647] co-purified with p ... | 1994 | 8013661 |
| expression of phytochrome apoprotein from avena sativa in escherichia coli and formation of photoactive chromoproteins by assembly with phycocyanobilin. | phytochrome dnas from oat (avena sativa l.) encoding the full-length 124-kda polypeptide, a 118-kda fragment lacking the first 65 amino acids, and two n-terminal fragments of 65 kda and 45 kda were subcloned and expressed in escherichia coli. reducing the temperature to 25 degrees c during cell growth and the coexpression of chaperones improved the folding into a functional conformation for most of the polypeptides, and in one case the yield of polypeptides was also enhanced. a maximum yield of ... | 1994 | 8033910 |
| effects of early experience on voluntary intake of low-quality roughage by sheep. | this study determined 1) how experience early in life with cured weeping lovegrass (eragrostis curvula) (low-quality roughage) or fresh oats (avena sativa) (high-quality roughage) affected consumption of low- or high-quality roughage by lambs later in life and 2) whether this early dietary experience affected passage of undigested residues through the digestive tract and digestion of low-quality roughage. from 1 to 5 mo of age, experienced lambs (el) were grazing cured weeping lovegrass, whereas ... | 1994 | 8056663 |
| the structure of the rdna intergenic spacer of avena sativa l.: a comparative study. | the sequence of the 18s-25s ribosomal rna gene intergenic spacer (igs) of avena sativa was determined. dna was cloned after polymerase chain reaction amplification of the igs. the spacer of 3980 bp is composed of non-repeated sequences and five tandem arrays of repeated sequences, named a to e. homology between oats igs and other grass species was found. tandem arrays d and e seem to be originated by duplication from single-copy sequences in related species. a transcription initiation site and p ... | 1994 | 8061326 |
| purification and characterization of an alpha-l-arabinofuranosidase from streptomyces lividans 66 and dna sequence of the gene (abfa). | the gene encoding an alpha-l-arabinofuranosidase (abfa) was homologously cloned in streptomyces lividans and its dna sequence was determined. the enzyme was purified from the cytoplasm of the hyperproducing clone s. lividans iaf116. its m(r) was estimated by gel filtration and found to be approx. 380,000. since sds/page indicated a native protein of m(r) 69,000, it can be concluded that the native protein consists of several subunits of that size. the pi value was 4.6. the kinetic constants dete ... | 1994 | 8092996 |
| chaperonin-mediated reconstitution of the phytochrome photoreceptor. | the native phytochrome photoreceptor was purified to homogeneity from etiolated seedlings of oat (avena sativa l.) and used for renaturation experiments. light scattering measurements showed that the groel molecular chaperone interacts with non-native phytochrome to suppress aggregation of the refolding polypeptide, following its dilution from a chaotrope. the binary complex formed between non-native phytochrome and groel was stable and could be isolated by size exclusion chromatography. dischar ... | 1993 | 8095267 |
| evidence that the alpha a gene of barley stripe mosaic virus encodes determinants of pathogenicity to oat (avena sativa). | complementary dna clones from which infectious rna can be transcribed were used to map the genetic determinants for oat (avena sativa l.) pathogenicity of barley stripe mosaic virus (bsmv). pseudorecombinant analysis of bsmv strains nd18 (nonpathogenic to oat) and cv42 (pathogenic to oat) indicated that the ability to systemically infect oat mapped to rna alpha. a homologous recombinant of nd18 (18 alpha tktkin), possessing nucleotides 2218 to 2454 from cv42 rna alpha, induced symptoms on oat si ... | 1994 | 8178475 |
| [a comparative ecological-genetic assessment of the mutagenic background in 2 agricultural districts of the transcarpathian province]. | the estimation of the total mutagenic overloading and chromosome variability in population of two transcarpathian regions in ukraine differing in the intensity of pesticides use has been studied. the statistically significant differences in mutagenicity of soil (on the growing seed allium cepa), mutability of agricultural crops (vicia sativa, avena sativa) and chromosome aberrations level in peripheral lymphocytes in juveniles have been determined. | 1993 | 8316952 |
| in situ localization of faba bean and oat legumin-type proteins in transgenic tobacco seeds by a highly sensitive immunological tissue print technique. | we have used a highly sensitive immunological tissue print technique to study cell- and tissue-specific expression of heterologous genes in transgenic plants. primary polyclonal antibodies, raised against legumin of faba bean (vicia faba l.) and 12s globulin of oat (avena sativa l.) were used to localize these proteins in transgenic tobacco seeds in a streptavidin-alkaline phosphatase assay in combination with biotinylated secondary antibodies producing a higher sensitivity (by several amplifica ... | 1993 | 8400128 |
| fourier-transform infrared spectroscopy of phytochrome: difference spectra of the intermediates of the photoreactions. | the photocycle of 124 kda phytochrome a from avena sativa was studied by fourier-transform infrared spectroscopy at low temperatures. difference spectra between the parent state pr and the intermediates of the pr-->pfr pathway, i.e. lumi-r, meta-ra, and meta-rc, and between pfr and the intermediates of the pfr-->pr pathway, lumi-f and meta-f, were obtained in 1h2o and 2h2o for the first time. each spectrum shows characteristic spectral features which allow a clear distinction between the differe ... | 1996 | 8718870 |
| persistence of the effects of early experience on consumption of low-quality roughage by sheep. | the aim of this study was to determine the effects of experience early in life with cured weeping lovegrass (eragrostis curvula) (low-quality roughage) or fresh oats (avena sativa) (high-quality roughage) on voluntary intake and nitrogen retention when sheep ate a low-quality roughage 9 mo after initial exposure. from 1 to 5 mo of age, experienced wethers (ew) grazed cured weeping lovegrass, whereas inexperienced wethers (iw) grazed fresh oats (initial exposure). then both ew and iw were fed sor ... | 1996 | 8726727 |
| purification and characterization of an oat fructan exohydrolase that preferentially hydrolyzes beta-2,6-fructans. | oat (avena sativa cv fulghum) fructan hydrolase was purified by ammonium sulfate precipitation and anion-exchange, hydrophobic interaction, and size-exclusion chromatography. the enzyme was purified to homogeneity as determined by the presence of a single band (43 kd) on a silver-stained sodium dodecyl sulfate-polyacrylamide gel. a mixture of beta-2,6-linked fructan (neokestin) isolated from oat was used as the substrate to purify fructan hydrolase. neokestin and small degree of polymerization f ... | 1996 | 8742337 |
| a calcium and free fatty acid-modulated protein kinase as putative effector of the fusicoccin 14-3-3 receptor. | a protein kinase that is activated by calcium and cis-unsaturated fatty acids has been characterized from oat (avena sativa l.) root plasma membranes. the kinase phosphorylates a synthetic peptide with a motif (-r-t-l-s-) that can be phosphorylated by both protein kinase c (pkc) and calcium-dependent protein kinase (cdpk)-type kinases. calphostin c and chelerythrine, two pkc inhibitors, completely inhibited the kinase activity with values of inhibitor concentration for 50% inhibition of 0.7 and ... | 1996 | 8754686 |
| isolation and expression of a host response gene family encoding thaumatin-like proteins in incompatible oat-stem rust fungus interactions. | four cdna clones (corresponding to tlp-1, -2, -3, and -4 genes) encoding thaumatin-like (tl), pathogenesis-related proteins were isolated from oat (avena sativa) infected by an incompatible isolate pga-1h of the oat stem rust fungus (puccinia graminis f. sp. avenae). all four cdna clones contained an open reading frame predicted to encode a 169-amino acid polypeptide with a signal peptide of 21 amino acids at the n-terminus, suggesting that these proteins are transported through a secretory path ... | 1996 | 8755626 |
| cloning and characterization of a highly repeated dna sequence in hordeum vulgare l. | a novel repetitive dna sequence, r10hvcop, has been identified in the barley (hordeum vulgare l.) genome. this 830 base pair (bp) dna sequence has a 606-bp open reading frame and is present as approximately 1.96 x 10(5) copies per haploid barley genome. southern blot analysis revealed that repetitive dna elements containing r10hvcop and related sequences were dispersed within the barley chromosomes. sequences similar to r10hvcop were also found in wheat (triticum aestivum l.), rye (secale cereal ... | 1996 | 8983185 |
| inducible overexpression of oat arginine decarboxylase in transgenic tobacco plants. | to test the possible interaction of polyamines in plant growth responses, transgenic tobacco plants containing the avena sativa l. (oat) arginine decarboxylase (adc) gene under the control of a tetracycline-inducible promoter were generated. inducible overexpression of oat adc in transgenic tobacco led to an accumulation of adc mrna, increased adc activity and changes in polyamine levels. transgenic lines, induced during vegetative stage, displayed different degrees of an altered phenotype, the ... | 1997 | 9107036 |
| oat-maize chromosome addition lines: a new system for mapping the maize genome. | novel plants with individual maize chromosomes added to a complete oat genome have been recovered via embryo rescue from oat (avena sativa l., 2n = 6x = 42) x maize (zea mays l., 2n = 20) crosses. an oat-maize disomic addition line possessing 21 pairs of oat chromosomes and one maize chromosome 9 pair was used to construct a cosmid library. a multiprobe (mixture of labeled fragments used as a probe) of highly repetitive maize-specific sequences was used to selectively isolate cosmid clones conta ... | 1997 | 9108009 |
| purification and characterization of recombinant affinity peptide-tagged oat phytochrome a. | full-length avena sativa (oat) phytochrome a (asphya) was expressed in the yeast saccharomyces cerevisiae and purified to apparent homogeneity. expression of an asphya cdna that encoded the full-length photoreceptor with a 15 amino acid 'strep-tag' peptide at its c-terminus produced a single polypeptide with a molecular mass of 124 kda. this strep-tagged polypeptide (asphya-st) bound tightly to streptavidin agarose and was selectively eluted using diaminobiotin, with a chromatographic efficiency ... | 1997 | 9114754 |
| aneuploid marker assignment in hexaploid oat with the c genome as a reference for determining remnant homoeology. | nullisomic lines of hexaploid oat avena sativa l. (2n = 6x - 2 = 40, aaccdd) cultivar sun ii were used to assign 134 dna sequences to 10 chromosome-associated syntenic groups. a limited set of ditelosomic lines allowed localization of subsets of these sequences to six chromosome arms. advantages of using such aneuploids in mapping are in the assignment of gene families, monomorphic rflp sequences, and oat linkage groups to chromosomes. the published hexaploid oat rflp linkage map has 38 linkage ... | 1997 | 9202416 |