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purification and properties of isoenzymes of cinnamyl-alcohol dehydrogenase from soybean-cell-suspension cultures.two isoenzymes of an nadp+ -dependent cinnamyl alcohol dehydrogenase and an nad+ - dependent aliphatic alcohol dehydrogenase were extracted from cell suspension cultures of soybean (glycine max l., var. mandarin) which form lignin during growth. these enzymes could be separated from each other by chromatography on deae-cellulose and hydroxyapatite. the cinnamyl alcohol dehydrogenase isoenzymes were partially purified by (nh4)2so4 fractionation, and column chromatography on deae-cellulose, sephad ...19751250
oxidative decarboxylation of para-hydroxybenzoic acids by peroxidases under in vivo and in vitro conditions.oxidative decarbyxylation of p-hydroxybenzoic acids in plant cell suspension cultures is catalyzed by peroxidases. this reaction has been characterized in vivo and in vitro. decarboxylation of substituted benzoic acids yields monomeric, dimeric and oligomeric benzoquinones. all peroxidases obtained from soybean (glycine max) cell suspension cultures by gel electrophoresis are equally capable to decarboxylate p-hydroxygenzoic acids as indicated by their rather low differences in specific activity ...20163046
enzymic synthesis of lignin precursors. purification and properties of a cinnamoyl-coa: nadph reductase from cell suspension cultures of soybean (glycinemax).a cinnamoyl-coenzyme a reductase catalyzing the nadph-dependent reduction of substituted cinnamoyl-coa thiol esters to the corresponding cinnamaldehydes was isolated from cell suspension cultures of soybean (glycine max l. var. mandarin). a 1660-fold purification of the enzyme was achieved by (nh4)2so4 fractionation, chromatography on deae-cellulose, hydroxyapatite and sephadex g-100 and affinity chromatography on 5'-amp-sepharose. the apparent molecular weight of the reductase was found to be a ...19767454
alpha-d-galactosidase from soybeans destroying blood-group b antigens. purification by affinity chromatography and properties.alpha-d-galactosidase was isolated from untoasted soybean meal and purified to homogeneity by affinity chromatography on n-epsilon-aminoacaproyl alpha-d-galactopyranosylamine-sepharose. the purified enzyme destroyed the b-specificity of human ovarian cyst b-glycoprotein with an accompanying increase in h-specificity, and converted human type-b erythrocytes to type o. the enzyme consists primarily of a tetramer, molecular weight 150 000 +/- 5 000 at ph 4.0 and of a monomer, molecular weight 40 00 ...197719253
physiological and biochemical studies on senescing tap root nodules of soybeans.senescence of soybean (glycine max l. merr.) tap root nodules was investigated by comparing changes in various physiological and biochemical activities with changes in capacity to fix nitrogen. field-grown beeson and calland varieties of soybeans of various ages were sources of tap root nodules. with both varieties, the number of tap root nodules per plant remained constant between 56 and 86 days after planting but fresh weight, dry weight, and mass of tap root nodules increased duing this perio ...199821737
electron-paramagnetic-resonance studies of leghaemoglobins from soya-bean and cowpea root nodules. identification of nitrosyl-leghaemoglobin in crude leghaemoglobin preparations.1. leghaemoglobins from soya-bean (glycine max) and cowpea (vigna unguiculata) root nodules were purified by chromatography on deae-cellulose phosphate columns at ph8.0 and ph5.8, to avoid the relatively low ph (5.2) commonly used to purify these proteins. 2. e.p.r. (electron-paramagnetic-resonance) spectra of the fluoride, azide, hydroxide and cyanide complexes of these ferric leghaemoglobins were very similar to the spectra of the corresponding myoglobin derivatives, indicating that the immedi ...197723110
effect of lime-pelleting on nodulation and yield of soybean grown in acid soil.an experiment for studying the effect of lime-pelleting of inoculated seed of soybean glycine max. (l.) merr. on nodulation, growth, and grain yield was undertaken in red sandy loam of bangalore, having a ph of 4.0. the results indicated that inoculation alone increased significantly nodulation, plant dry weight, and grain yield. but inoculation plus lime-pelleting significantly increased the dry weight of nodules and plants, but not the grain yield. however, lime-pelleting was found to be benef ...197724961
inhibition of ige and compound 48/80-induced histamine release by lectins.lectins from ricinus communis and glycine max, as well as wheat germ agglutinin and concanavalin a, caused a dose-dependent release of histamine from mast cells present in the mixed peritoneal cells from the rat. in addition, histamine release in an ige-mediated and a compound 48/80-mediated reaction was inhibited in cells which had been pretreated with these lectins. with concanavalin a and the r. communis lectin both effect were prevented by the addition of the appropriate monosaccharides to t ...197551003
purification, partial characterization, and immunological relationships of multiple low molecular weight protease inhibitors of soybean.five protease inhibitors, i--v, in the molecular weight range 7000--8000 were purified from tracy soybeans by ammonium sulfate precipitation, gel filtration on sephadex g-100 and g-75, and column chromatography on deae-cellulose. in common with previously described trypsin inhibitors from legumes, i--v have a high content of half-cystine and lack tryptophan. by contrast with other legume inhibitors, inhibitor ii contains 3 methionine residues. isoelectric points range from 6.2 to 4.2 in order fr ...197773387
the unlabelled-peroxidase antiperoxidase method used for the localization of leghaemoglobin in glycine max nodules [proceedings]. 199993466
significance for humans of biologically active factors in soybeans and other food legumes. 199994068
stability of polysome-associated polyadenylated rna from soybean suspension culture cells.the half-life of polysome-associated, poly(a)-rna in exponentially growing soybean (glycine max) suspension culture cells was determined with pulse-chase experiments. based on a best fit from a computer analysis of the data, two decay components for poly(a)-rna were found. one component had a half-life of approximately 0.6 h, while the other had a half-life of about 30 h, similar to the doubling time of the cultures. at the beginning of the chase period, the short-lived component represented app ...1998106874
circular dichroism of lipoxygenase-1 from soybeans.the circular dichroism spectra of the three forms of lipoxygenase-1 from soybeans show characteristic differences in the region between 300 and 600 nm. native lipoxygenase-1 only shows a negative dichroic band around 330 nm. yellow lipoxygenase-1, obtained by addition of an equimolar amount of 13-f-hydroperoxylinoleic acid to the native enzyme, shows a positive cotton effect at 425 nm, while the negative band band at 330 nm has increased in intensity. the blue enzyme, representing a complex of y ...2001114226
co-oxidation of carotenes requires one soybean lipoxygenase isoenzyme.the type ii lipoxygenase (optimum ph 6.5) from soybeans was purified and separated into two fractions either by chromatography on deae-sephadex or by isoelectric focusing. in the presence of linoleic acid and oxygen both fractions co-oxidise canthaxanthine or beta-carotene as effectively as a combination of these fractions. oxygenation of linoleic acid and co-oxidation of canthaxanthine by type ii lipoxygenase is stimulated by 13-hydroperoxy-cis-9,trans-11-octadecadienoic acid but not by 13-hydr ...1979117839
9-lr-linoleyl hydroperoxide, a novel product from the oxygenation of linoleic acid by type-2 lipoxygenases from soybeans and peas.type-2 lipoxygenases from soybeans and peas, which have a ph optimum of 6--7 were examined for oxygenation activity at ph 9.0. the reaction velocity was found to be strongly dependent on substrate concentration. at higher substrate concentrations an inhibitory effect was observed, which is connected with the occurrence of a kinetic lag phase. on incubation of linoleic acid at ph 9.0 with either of these enzymes predominantly 9-lr-hydroperoxy-10-trans,12-cis-octadecadienoic acid is formed. the si ...1979117841
silymarin, an inhibitor of lipoxygenase.silybin (i), silydianin (ii) and silychristin (iii), the constituents of silymarin, non-competitively inhibit the lipoxygenase from soybeans (ec 1.13.11.12) in vitro.1979118048
nitrogen fixing activity in rhizobium japonicum separated from plant cell cultures.induced by soy bean tissue cultures in socalled "tissue chambers", rhizobium japonicum str. 61-a-96 developed nitrogenase activity separated from the plant cells. the activity proceded for 48 h with a rate of 1 x 10(-8) nmol c2h4h-1 cell-1, which is about 6% of the activity measured for bacteroids from rhizobium japonicum in nodules of glycine max.1975130001
phospholipid-protein interactions in the ca2+-adenosine triphosphatase of sarcoplasmic reticulum.ca2+-adenosine triphosphatase from sarcoplasmic reticulum has been delipidated by gel filtration through a sephadex g-200 column equilibrated with buffer containing cholate. the delipidated ca2+-adenosine triphosphatase had negligible adenosine triphosphatase activity, but up to 50% of the atpase activity was restored when the delipidated enzyme was recombined with phosphilipids. it was shown with the delipidated preparation that the phosphorylation of the enzyme by either atp or pi was entirely ...1976134036
the organization of a nuclear dna sequence from a higher plant: molecular cloning and characterization of soybean ribosomal dna.the recombinant dna vector, lambda charon 4a, was used to construct a library of dna sequences from the genomic dna of soybean (glycine max). to define the organization of ribosomal dna (rdna) in the soybean genome, clones containing sequences complementary to both 17s and 25s rrna have been isolated from this library and used in conjunction with southern blot hybridization. the rrna genes are tandemly reiterated with a relatively small unit repeat length of 7.8 kb. there is no heterogeneity in ...1979160361
relationship between enhanced turnover of phosphatidylinositol and lymphocyte activation by mitogens.1. various lectins [phaseolus vulgaris phytohaemagglutinin, glycine max (soy-bean) agglutinin, triticum vulgaris (wheat-germ) agglutinin and axinella polyploides agglutinin] and antibodies to pig ig (immunoglobulin) that are found by pig lymphocytes were assessed in terms of their capacities to stimulate lymphocyte transformation and to enhance phosphatidylinositol turnover. transformation was measured after 45h of culture by incorporation of [6-(3)h]thymidine into dna, whereas phosphatidylinosi ...1975167716
lipid metabolism in germinating seeds. purification of ethanolamine kinase from soya bean.ethanolamine kinase has been purified to homogeneity from germinating soya bean (glycine max l.) seeds. the purified enzyme had a molecular weight of 17--19 000 as estimated by gel filtration and sodium dodecyl suphate-polyacrylamide gel electrophoresis. it would not phosphorylate choline, had a km for ethanolamine of 8 microm and utilised mg-atp. the kinase could be purified in a 37 000 molecular weight form (dimer) which would easily dissociate on storage. in contrast to ethanolamine kinase wh ...1979228735
[treatment of hyperlipidemia type ii with soybeans].15 outpatients with type ii hyperlipoproteinemia (7 with type iia and 8 with type iib) were treated with soybean. this diet induced a significant decrease of plasma cholesterol levels from 314 +/- 24 to 267 +/- 30 mg/100 ml. triglycerides levels remained unchanged. ldl-cholesterol decreased significantly from 241 +/- 26 to 194 +/- 32 mg/100 ml, whereas hdl-cholesterol increased only slightly and not significantly from 38 +/- 7 to 40 +/- 8 mg/100 ml. in our efforts to reduce hyperlipidemia the ve ...1979231304
ammonia assimilation by rhizobium cultures and bacteroids.the enzymes involved in the assimilation of ammonia by free-living cultures of rhizobium spp. are glutamine synthetase (ec. 6.o.i.2), glutamate synthase (l-glutamine:2-oxoglutarate amino transferase) and glutamate dehydrogenase (ed i.4.i.4). under conditions of ammonia or nitrate limitation in a chemostat the assimilation of ammonia by cultures of r. leguminosarum, r. trifolii and r. japonicum proceeded via glutamine synthetase and glutamate synthase. under glucose limitation and with an excess ...1975234505
isoenzymes of p-coumarate: coa ligase from cell suspension cultures of glycine max.two isoenzymes of p-coumarate: coa ligase were isolated from cell suspension cultures of soybean (glycine max l., var. mandarin). separation and partial purification of the enzymes were achieved by precipitation with mncl2 and (nh4)2so4, and column chromatography on deae-cellulose, sephadex g-100 and hydroxyapatite. the isoenzymes had approximately the same molecular weight, but differed significantly with respect to their substrate specificity, their inhibition constants for amp, their dependen ...2003240682
adsorption and selection of rhizobia with ion-exchange papers.ion exchange papers were used to study the adsorption of 32p-labelled rhizobia on defined surfaces. two strains of rhizobium japonicum and one each of r. leguminosarum and r. lupini were compared with escherichia coli and bacillus subtilis. the ratio of adsorption to strong and to weak acid papers/strong and weak basic papers was consistantly higher for all rhizobial strains compared to the other bacteria. the process of desorption by increasing the ion-concentration causes about 35% desorption ...1975242293
autotrophic growth of h2-uptake-positive strains of rhizobium japonicum in an atmosphere supplied with hydrogen gas.previous research from this laboratory has demonstrated co(2)-fixing and h(2)-uptake capacities of certain strains of rhizobium japonicum. in this report we have shown that sr, a h(2)-uptake-positive (hup(+)) strain of r. japonicum, is capable of autotrophic growth with h(2) as the energy source. growth occurred on mineral salts/vitamins/noble agar, mineral salts/vitamins liquid medium (0.27 mug of c as vitamins per ml), and in mineral salts liquid medium with no added vitamins when cultures wer ...1979287019
elastases from human and canine granulocytes, ii. interaction with protease inhibitors of animal, plant, and microbial origin.inhibitors of animal, plant, and microbial origin were tested against human and canine granulocytic elastases. the trypsin-chymotrypsin inhibitors from dog submandibular glands, from soybeans (bowman-birk) and from chickpeas show strong interaction with these proteases (ki = 10(-8) - 10(-9)m). the trypsin-kallikrein inactivator of bovine organs (trasylol) is not active against granulocytic elastases or against human granulocytic cathepsin g. elastatinal, a specific inhibitor of elastases, isolat ...1977300070
circular dichroism studies on the effects of ethanol on the conformation of alpha1-acid glycoprotein, alpha1-antitrypsin, deoxyribonuclease, pepsinogen, soybean trypsin inhibitor and unfolded ribonucleases.the effects of 25 to 75 volume-% ethanol on conformation of human serum alpha1-acid glycoprotein, human serum alpha1-antitrypsin, pancreatic deoxyribonuclease i, porcine pepsinogen, the "kunitz" trypsin inhibitor from soybeans, and oxidized as well as reduced and s-carboxymethylated ribonucleases were tested by the circular dichroism (cd) probe. it was found that 25 volume-% ethanol had a slight effect, whereas 50--75 vol.-% alcohol significantly altered the conformation. the tertiary structure ...1978306838
potential for the use of germinated wheat and soybeans to enhance human nutrition.wheat and soybeans are the major agricultural exports of the united states. the u.s. sells more of each crop than any other nation. soybeans are the main staple in china, but the u.s. sells more soybeans than china grows. for hundreds of millions of other people, wheat is the main staple. and yet, most americans eat whole grains of neither wheat nor soybeans. in the united states, many nutrients of wheat and soybeans are lost in processing or are fed to animals. a highly significant share of the ...1978364942
somatic mosaicism in plants with special reference to somatic crossing over.plant systems in use for the detection of environmental mutagens appear capable of detecting all types of genetic effects which can be studied in animals. the study of somatic mosaicism, however, is better developed in plants than in higher animals. a case is presented here which shows the ability of plant systems in analyzing a host of genetic end points, including chromosome aberrations like deletions, somatic crossing over, numerical inequality, gene conversion, paramutations and point mutati ...1978367771
[soybeans and human nutrition]. 1979390878
proteinase inhibitors in severe inflammatory processes (septic shock and experimental endotoxaemia): biochemical, pathophysiological and therapeutic aspects.plasma levels of antithrombin iii, alpha 2-macroglobulin and inter-alpha-trypsin inhibitor, as well as those of various clotting, complement and other plasma factors, were significantly decreased in 18 patients suffering from hyperdynamic septic shock. a similar statistically significant reduction of the concentrations of several plasma factors (prothrombin and antithrombin iii, plasminogen and alpha 2-plasmin inhibitor, complement factor c3 and clotting factor xiii) was observed in experimental ...1979399895
[thermal inactivation and storage behavior of technologically important enzymes. ii. lipase from geotrichum candidum and lipoxygenase from soybeans].the thermal inactivation of lipase out of geotrichum candidum and of lipoxigenase out of soybeans was investigated in phosphate buffer solution. comparable to peroxidase, a sharp bend was observed in the inactivation curves of both enzymes. the z-value of 19 degrees c for lipase out of geotrichum candidum was higher than the values indicated in the pertinent literature for pancreas lipase and milk lipase. the z-value for soy lipoxigenase was found to be 9.8 degrees c.1977408985
biosynthesis of a prostaglandin by a plant enzyme.lipoxygenase-2 from soybeans catalyzes the oxygenation of arachidonic acid to form significant amounts of a prostaglandin product. results obtained with the dithionite-reduced derivatized product upon combined gas chromatography-mass spectroscopy are consistent with structures of the stereoisomers of 9,11,15-trihydroxyprosta-5,13-dienoic acid. a portion of the material formed by reduction of the enzyme product with dithionite reacts positively in a radioimmunoassay against rabbit anti-prostaglan ...1978412847
deoxyribonucleotide synthesis and dna polymerase activity in plant cells (vicia faba and glycine max).enzymes of deoxyribonucleotide and dna biosynthesis, which are little known in plants, were studied in root tips of germinating broad beans (vicia faba) and in fast-growing cultures of soybean cells (glycine max). the plant cells contain a ribonucleoside 5'-diphosphate reductase which is detected in vitro only during a limited period of growth, viz. 30--32 h after inhibition of vicia seeds, and between the second and third day after inoculation of soybean cultures. in both species ribonucleotide ...1979420854
mycological survey of selected health foods.a survey was conducted to compare the total viable fungal content and the number of different mold species encountered in 10 types of health foods labeled organically grown and in the same foods without such a label. the foods were wheat flour, corn meal, brown rice, figs, split peas, pinto beans, soybeans, walnuts, pecans, and peanuts. results showed no consistent difference in either the total viable fungal content or the number of different mold species encountered between the labeled and unl ...1979453829
conversion of glycine max seed agglutinins from nonspecific to anti-(a + b).the seeds of glycine max contain agglutinins which are typically nonspecific in their reactivity. our investigations show that the phytagglutinins in gm can be converted from nonspecific to anti-(a + b) after the lectin is absorbed with horse red cells. the anti-a and anti-b fractions can be further separated by suitably absorbing the lectin with human red cells. the lectin absorbed with horse red cells or with group-0 human red cells shows an a-stressed activity.1979488297
[lectins as reagents for the differentiation of serum enzymes. lectins as reagents, i. (author's transl)].lectins from canavalia ensiformis, phaseolus vulgaris, and triticum vulgare react with arylamidase, alkaline phosphatase, gamma-glutamyltransferase, and cholinesterase of human sera by formation of enzymatically active, mostly insoluble complexes. arylamidase, alkaline phosphatase, and cholinesterase react more intensely in sera of healthy people than in sera of patients with liver and neoplastic diseases. arylesterase is bound to a distinct degree only by concanavalin a. the enzymes mentioned a ...1979547035
[purification and properties of prolidase for germinating soybeans (author's transl)]. 1977557088
direct plating versus dilution plating in qualitatively determining the mold flora of dried beans and soybeans.two methods presently used for examining whole foods and feeds for viable molds were evaluated for their relative effectiveness in the qualitative determination of the total number of mold species present in soybeans and dried beans: the direct plating method and the serial dilution method. sixty-nine soybean samples and 40 dried bean samples were examined. although the quantitative results of this study were inconclusive, the qualitative results show that the direct plating method was substanti ...1977558185
changes in phospholipid levels during high moisture storage of soybeans. 1977558242
isolectins from soybean (glycine max).the major lectin in seeds of a soybean cultivar (glycine max cv d68-127) has been purified to apparent homogeneity by hydroxyapatite and deae-cellulose chromatography. in the latter, the behavior of the lectin was similar to that of the minor isolectins previously described in other soybean cultivars. molecular weights of 92 000 for the molecule and 23 000 for the subunits were determined by gel filtration and sodium dodecyl sulfate-gel electrophoresis; these are smaller than those previously re ...1977558805
ultrastructure of soybean nodules. i: release of rhizobia from the infection thread.root nodules on soybeans (var. clark 63) were examined by transmission electron microscopy 10-12 days after seed inoculation and planting. the cell infection process appeared identical in both effective nodules, induced by rhizobium japonicum strain 138 (usda) and in ineffective nodules, induced by strain 8-0 (iowa). electron micrographs are presented which suggest that rhizobia are freed from the infection thread by disintegration of the thread wall and compartmentalization of the distintegrat ...1977559533
effect of rj1rj1 (non-nodulating) soybeans on nodulation of near isogenic rj1rj1 plants in nutrient culture.an earlier proposal (can. j. microbiol. 7: 851; 1961) that rj1rj1 (non-nodulating) soybeans (glycine max (l.) merr.) excrete a substance that inhibits nodulation of rj1 rj1 (nodulating) plants was tested. using near isogenic lines (isolines) of "clark" and "harosoy" soybeans, we consistently found nonsignificant reduction in nodule number and acetylene reduction per rj1rj1 plant grown in association with their rj1rj1 counterparts: these results suggest that a nodulation inhibitor is not associat ...1977560904
survey of 1975 wheat and soybeans for aflatoxin, zearalenone, and ochratoxin.wheat samples (102 lots) were collected from virginia, north carolina, southeastern missouri, southern illinois, and kentucky. soybean samples (180 lots) were collected from virginia, illinois, iowa, minnesota, nebraska, alabama, arkansas, and texas. samples of both commodities were analyzed for zearalenone, aflatoxin, and ochratoxin by the eppley method. none of the 3 mycotoxins was detected in soybeans. aflatoxins and ochratoxin a were not detected in wheat, but zearalenone was detected in 19 ...1977561049
sensory factors which affect the acceptance of raw and heated defatted soybeans by rats.the acceptability of heated soybean meal (hs) compared to raw soybean flakes (rs) was studied by means of diet selection in free choice situations. the selection by rats of diets containing hs over those containing rs was shown in short- and long-term preference tests. texture did not seem to be an important factor in the acceptability difference between hs and rs. rats selected a less nutritious diet containing rs + sodium saccharin (appealing taste) over a diet containing hs + sucrose octaacet ...1977561170
chronic mycotoxicosis in chicks caused by toxins from phomopsis grown on soybeans. 1977561808
[various aspects of production in the cultivation of soybeans]. 1977562139
determination of free monosaccharides and detection of sugar alcohols in mature soybean seeds.although the oligosaccharide contents of soybeans are well documented, the exact values of monosaccharide contents have not been reported. elaborate methods of preparative paper chromatography together with gas chromatography established the following data for one variety, kyushu no. 12. the air-dried cotyledon part (admixed with hypocotyls) contained 0.030% glucose and 0.039% fructose. the hull part contained 0.018% galactose, 0.028% glucose, 0.023% fructose, 0.005% arabinose, and 0.002% xylose ...1977562395
genetic control of symbiotic nitrogen fixation in soybeans. 1977562661
[effect of various processes on the protein quality of food based on corn and soybeans].the present study deals with the effect of various processing methods on the protein values of maisoy, a blend of 30% whole soybean and 70% corn. the processes evaluated were: a) lime cooking using calcium hydroxide; b) toasting, and c) extrusion cooking, using an equipment known as the brady crop cooker. the same blend of soybean/corn was processed by the same three methods. in addition, a sample of maisoy--blend produced in bolivia with the extrusion equipment previously indicated--was also ev ...1977563218
localisation and characterization of the fatty acid synthesizing system in cells of glycine max (soubean) suspension cultures.in course of a study of fatty acid synthetase in higher plants, non-green cell suspension cultures of glycine max (soybean) served as model tissues. for the first time, a fatty acid synthesizing system was characterized in cell cultures of higher plants and was found to be solely located in proplastids of the cells. optimum activity of the fatty acid synthesizing system in proplastids was observed between ph 8.0 and 8.2; with [1-14c]acetate as substrate, cofactors required were coa, atp, mn2+, m ...1977563244
production of vitamin b-12 in tempeh, a fermented soybean food.several varieties of soybeans contained generally less than 1 ng of vitamin b-12 per g. it was found that use of a lactic fermentation typical of tropical conditions during the initial soaking of the soybeans did not influence the vitamin b-12 content of the resulting tempeh. pure tempeh molds obtained from different sources did not produce vitamin b-12. it was found that the major source of vitamin b-12 in commercial tempeh purchased in toronto, canada, was a bacterium that accompanies the mold ...1977563702
residue determination of a dioxane herbicide in soil and soybeans by high-pressure liquid chromatography. 2006563881
selenium and arsenic levels in soybeans from different production regions of the united states. 2013563882
dieldrin residues in soybeans in illinois, 1965, 1966, 1967, 1971, and 1974.the illinois soybean crop was monitored in 1965, 1966, 1967, 1971, and 1974 for dieldrin residues resulting from aldrin applied to corn in the years before soybean cultivation. residue levels of dieldrin in soybeans increased between 1965 and 1974. the percent of fields which had soybeans with a dieldrin level above 0.03 ppm increased between 1965 and 1974. dieldrin residue levels in soybeans grown in illinois are expected to decline now that the use of aldrin has diminished and will soon cease. ...1977564021
comparative nutritive value, amino acid content, chemical composition, and digestibility in vitro of vegetable- and grain-type soybeans. 1978564917
grouping of rhizobial strains--a method based on symbiotic characteristics.twenty strains of rhizobium japonicum and non-inoculated control were used to study seven symbiotic characteristics with two varieties of soybean (glycine max). the strains were then grouped on the basis of these symbiotic characteristics, using mahalanobis' d2 statistical method. eight groups were formed in which two strains stood distinctly aloof, indicating thereby the exceptional nature of these strains over others in their symbiotic behaviour. this method is suggested for selecting exceptio ...1977565116
stepwise selection of efficient rhizobial cultures through cultural characteristics.nodulation and shoot nitrogen of two varieties of soybean (glycine max) were studied with twenty strains of rhizobium japonicum. a number of cultural characteristics of the strains in isolation to the symbiotic system were also studied. a stepwise selection method was employed for detecting efficient cultures through the cultural characteristics which showed association with the steps in the symbiotic system. nodulation of one variety was found to be associated with the dehydrogenase activity an ...1977565117
the influence of alachlor, trifluralin, and diazinon on the development of endogenous mycorrhizae in soybeans.preplant incorporated treatments of 2 and 4 kg/ha of trifluralin and diazinon had no significant effect on growth, p accumulation or root colonization by mycorrhizal fungi in soybeans planted in an andover clay loam. at 4 kg/ha, alachlor and trifluralin inhibited root development of 25 day-old plants. the 4 kg/ha alachlor treatment reduced shoot weight of 25 day old plants significantly and suppressed mycorrhizal development of 25 to 60 day old plants. at currently used commercial rates neither ...1978565229
separation of the isomeric isoflavones from soybeans by high-performance liquid chromatography. 1978565367
hydrogen evolution and uptake by nodules of soybeans inoculated with different strains of rhizobium japonicum.hydrogen evolved by nitrogenase may be recycled by a hydrogenase present in some legume nodules. anoka and portage cultivars of soybeans were inoculated with each of 8 and 24 strains, respectively, of rhizobium japonicum and surveyed for h2 evolution and c2h2 reduction rates nodule weight, and plant dry weight. six of the strains (3ilb 110, usda 122, usda 136, 3ilb 6, 3ilb 142, and 3ilb 143) which exhibited no h2 evolution in air were shown to take up h2. the relative efficiencies of nitrogenase ...1978565672
nutrient comparison of fresh and field-dried, green-seeded soybeans.nutrient composition and biologic utilization of cooked, dried, and ground meals prepared from fresh and field-dried, green-seeded edible soybeans were evaluated. on a dry-weight basis, nutrient content of the fresh and field-dried meals were comparable for protein, fat, calcium, phosphorus, magnesium, copper, and iron; fresh beans tended to have higher zinc content than the field-dried beans. nutrient values for the green-seeded soybean meals were comparable to published values for full-fat soy ...1978566776
changes in rat pancreatic protein synthesis after a single feeding with diets containing raw or heated soybeans.the patterns of protein synthesis following the administration of a single meal containing defatted ground soybeans (rsd) or heated defatted ground soybeans (hsd) were compared. a double label method was used so that the determination of the relative rate of synthesis of an enzyme could not be obscured by a possible alteration of the activity or quantity of the enzyme. rats were given one meal of rsd or hsd and were subsequently killed at different times after the meal. eight hours after the mea ...1978566785
somatic crossing-over in glycine max (l.) merrill: activation of dimethyl nitrosoamine by plant seed and comparison with methyl nitrosourea in inducing somatic mosaicism.the soybean system used for detecting environmental mutagens is analyzed for various types of spots on the leaves of heterozygous y11y11 plants and homozygous y11y11's induced by a nitrosoamine (dimethyl nitrosoamine, dmn) and a nitrosoamide (methyl nitrosourea, mnu). it is shown that the nitrosoamine can be "activated" by the seed (is converted to a true mutagen) without the addition of nadph or s-9 fraction of the liver homogenate as is necessary in animal tissue culture or bacterial studies. ...1978569252
improvement of the protein quality of corn with soybean protein.in most central american countries, lime-treated corn provides 31% of the total protein and 45% of the energy intake, and beans 24% of the protein and 12% of the calories. such diet is low in protein quality and quantity, as well as in energy. to overcome these deficiencies, corn can be supplemented either with its limiting amino acids, lysine and tryptophan, or better still, with whole soybeans which improve not only the amount and quality of the protein consumed but, because of their high oil ...1978569424
soy protein utilization in food systems.soy protein products are utilized in food systems as whole beans, flours and grits, concentrates and isolates, and textured products. soy proteins play a significant role in food systems as a source of supplementary and complementary protein and contribute functional properties such as solubility, water absorption, viscosity, emulsification, texture, and antioxidation. whole soybeans are processed into snack foods, beverages, and fermented foods. soy protein is an ideal supplement for cereal pro ...1978569429
evaluation of sclerotinia sclerotiorum as a potential mycotoxin producer on soybeans.solvent extracts of sclerotinia sclerotiorum sclerotia were nontoxic to mice and chicken embryos; psoralens were not detected. solvent extracts of soybeans inoculated with 10 strains of s. sclerotiorum were toxic on injection but nontoxic on per os administration to mice. the presence of chlorinated hydrocarbons in the soybeans may partially help explain toxicity by intraperitineal injection.1978569464
gas-liquid chromatographic determination of sodium 5-[2-chloro-4-(trifluoromethyl)phenoxy]-2-nitrobenzoate residues on soybeans and foliage, soil, milk, and liver.residues of sodium 5-[2-chloro-4-(trifluoromethyl)phenoxy]-2-nitrobenzoate and its free acid are determined by treating a sample extract with diazomethane to convert the residues to methyl 5-[2-chloro-4-(trifluoromethyl)phenoxy]-2-nitrobenzoate. this compound is purified by chromatography on florisil and measured by electron capture gas-liquid chromatography. the method has been used on soybeans, soybean foliage, soil, milk, and liver. it can detect 0.01 ppm with recoveries of 70--75%.1978569657
distribution of chlorinated pesticides in soybeans, soybean oil, and its by-products during processing. 1978569670
genetically marked rhizobium identifiable as inoculum strain in nodules of soybean plants grown in fields populated with rhizobium japonicum.the fate of an inoculum strain of rhizobium japonicum was studied using a genetically marked strain i-11o subline carrying resistance markers for azide, rifampin, and streptomycin (i-110 ars). at the time of planting into a field populated with r. japonicum, seeds of soybean cultivars kent and peking were inoculated with varying cell densities of strain i-110 ars. at various times during the growing season, surface-sterilized root nodules were examined for the presence of the inoculum strain by ...1978570015
bacteriocin-like substances produced by rhizobium japonicum and other slow-growing rhizobia.bacteriocin-like substances were commonly produced by slow-growing rhizobium japonicum and cowpea rhizobia on an l-arabinose medium. antagonism between strains of r. japonicum was not detected in vitro; however, such strains were often sensitive to some bacteriocins produced by cowpea rhizobia. inhibitory zones (2 to 8 mm from colony margins), produced by 58 of 66 r. japonicum test strains, were reproducibly detected with corynebacterium nebraskense as an indicator. quantitative production was n ...1978570016
sequence organization of the soybean genome.the total complexity of one constituent soybean (glycine max) genome is estimated to be 1.29 . 10(9) nucleotide pairs, as determined by analysis of the reassociation kinetics of sheared (0.47 kilobase) dna. single copy sequences are estimated to represent from 53 to 64% of the genome by analysis of hydroxyapatite binding of repetitive dna as a function of fragment length. from 65 to 70% of these single copy sequences have a short period interspersion with 1.11--1.36 kilobase lengths alternating ...1979570420
inhibition of human and rat pancreatic proteinases by crude and purified soybean proteinase inhibitors.effects of proteinase inhibitors on total proteolytic activity and trypsin and chymotrypsin activity in human pancreatic juice were determined separately. purified inhibitors as well as crude extracts of raw soybeans completely inhibited trypsin and chymotrypsin activity while 40 to 50% of the total proteolytic activity remained. inhibition experiments with 1,10-o-phenanthroline showed that this residual proteolytic activity was due mainly to carboxypeptidase a and b. comparative studies with ra ...1979571012
the biosynthesis of ribulose bisphosphate carboxylase. uncoupling of the synthesis of the large and small subunits in isolated soybean leaf cells.isolated leaf cells from soybean (glycine max) incorporate [35s]methionine into protein at a linear rate for at least 5h. analysis of the products of incorporation by one-dimensional and two-dimensional polyacrylamide gel electrophoresis shows that major products are the large and small subunits of the chloroplast enzyme, ribulose bisphosphate carboxylase. the large subunit is synthesized by chloroplast ribosomes and the small subunit by cytoplasmic ribosomes. addition of chloramphenicol to the ...1979571334
the nutritive value of dehulled soybeans fermented with aspergillus oryzae or rhizopus oligosporus as evaluated by rats. 1979571903
cross-polarization nmr of n-15 labeled soybeans. 1979572223
production and nutritive value of soybeans.soybean world production has been increasing at a rate of 5.2% per year (average yield is around 1,400 kg/ha). this production has been solely used for oil extraction and the protein meal obtained for animal rations, but lately it is being used for human consumption. brazil, the third largest producer, has had a yearly rate of production increase of 32% in the last years. average yields in brazil are still low (around 1,500 kg/ha), but in experimental results, yields over 3,000 kg/ha have been o ...1978572663
carbohydrate metabolism in chicks after administration of toxins from a phomopsis sp.soybeans inoculated with phomopis sp. were incubated for weeks and then extracted with methanol and ether, and the culture filtrates were intubated into crops of chicks. activities of glucose-6-phosphatase and glucose-6-phosphate dehydrogenase in liver were increased, and glycogen stores and plasma glucose concentrations were reduced. histologically, severe hepatocytic necrosis was found in chicks treated with the toxic culture filtrate. significant clinical signs were loss of body weight and re ...1979573612
pinitol, a larval growth inhibitor for heliothis zea in soybeans.a search for insect growth inhibitors in methanol extracts of soybean leaves resulted in isolation of pinitol. pinitol caused a 50% reduction in weight gain (ed50) of heliothis zea larvae at about 0.7% when added to a synthetic diet. although myo-inositol is a normal component of the insect diet, it also caused growth inhibition at higher concentrations; ed50 4%.1979573698
dissipation of 14c n-nitroso-di-n-propylamine from field soil and residue determinations in field-grown soybeans. 1979574034
influence of photoinduction on aminotransferase activity in biloxi soybean, glycine max. l., merr.increasing number of photoinductive cycles markedly influenced the activity of 5 different aminotransferase systems as compared to non-inductive cycles. the activity was related to the requirement of the corresponding aminoacid synthesised during the process of photoinduction in the leaves.1979574461
vesicular-arbuscular mycorrhiza and nodulation in soybean.dual infections of glycine max with va endophytes and rhizobium, compared with rhizobium alone, increased the number and weight of nodules significantly in natural field soil and obviated the need of phosphate application for successful nodulation.1979574481
studies on soybean trypsin inhibitors. x. isolation and partial characterization of four soybean double-headed proteinase inhibitors.four bowman-birk type double-headed inhibitors (b, c-ii, d-ii, and e-i) were isolated from soybeans. inhibitor b was different from bowman-birk inhibitor only in chromatographic behavior. one mole of c-ii inhibited one mole each of bovine trypsin and bovine alpha-chymotrypsin, probably at the same site, and porcine elastase at another reactive site. in the ordinary assay system d-ii and e-i inhibited only trypsin activity at a non-stoichiometric inhibitor-enzyme ratio of 1:1.4, and the complexes ...1977599140
studies on soybean trypsin inhibitors. xi. complete amino acid sequence of a soybean trypsin-chymotrypsin-elastase inhibitor, c-ii.soybean inhibitor c-ii, which inhibits trypsin, alpha-chymotrypsin, and elastase, was reduced and s-carboxymethylated, and digested with trypsin. the amino acid sequences of the resulting tryptic peptides were determined by conventional methods, establishing the complete 76-amino acid sequence of the inhibitor. inhibitor c-ii was found to be homologous with soybean (glycine max) bowman-birk inhibitor and more closely related to an inhibitor from garden beans (phaseolus vulgaris). the homology wi ...1977599141
hormone-mediated translational control of protein synthesis in cultured cells of glycine max. 1978627313
localization of carbohydrate components in rat colon with fluoresceinated lectins.cryostat sections of rat descending colon were studied by fluorescence microscopy after exposure to conjugates of fluorescein isothicoyanate with lectins from glycine max (soybean), triticum vulgaris (wheat germ), ricinus communis (castor bean), ulex europaeus, (gorse), dolichos biflorus (horse gram) and canavalia ensiformis (concanavalin a) (jack bean). no two lectins showed identical patterns of fluorescence. fitc-conjugates of soybean and d. biflorus lectins reacted strongly with the mucus pr ...1978670681
circular dichroism studies on the effects of sodium dodecyl sulfate on the conformation of some phytohemagglutinins.conformation and conformational transitions of phytohemagglutinins (lectins) from arachis hypogaea (peanut), lens culinaris (lentil), glycine max (soybean), and ricinus communis (castor bean) were studied by the circular dichroism probe. the lectins were found to possess a high amount of the pleated sheet (beta) conformation. the tertiary structure and main chain conformation of the lectins were disorganized by sodium dodecyl sulfate (sds) and the main chain was reconstructed by relatively high ...1978708761
circular dichroism spectra of isolated soybean and chickpea trypsin-chymotrypsin inhibitors.circular dichroism spectra of trypsin-chymotrypsin inhibitors from soybeans and chickpeas have been determined in acidic, neutral and highly alkaline solutions. neither protein contains alpha-helix although a small amount of beta-structure cannot be excluded. negative dichroism above 250 nm has been assigned largely to disulfide bonds in both molecules with neither showing evidence for tyrosine residues buried in hydrophobic regions. the spectra of these inhibitors between 230 and 250 nm have be ...1978744684
cummings memorial lecture - 1975. the market basket: food for thought.the world food crisis is as critical today as when it was debated at the 1974 world food conference in rome. since the united states and canada-and to a lesser extent, australia and new zealand-lead in the production of corn, wheat and soybeans, the north american "bread basket" has become the "market basket" of the world. for welfare, economic, and political reasons, our energies, resources, and deliberations must be expanded toward optimum production of wholesome food products. i do not recomm ...1975779439
hypertrophy and hyperplasia in the endocrine and exocrine pancreas of rats fed soybean trypsin inhibitor or repeatedly injected with pancreozymin.rats were 1) fed raw soybeans for 30 days, 2) given trypsin inhibitor as drinking water for 7 days, or 3) repeatedly injected with cholecystokinin-pancreozymin (cck-pz) for 7 days. after all these treatments the pancreas of the animals was markedly enlarged. microscopic examination revealed, besides hypertrophy of acinar cells, increased mitotic figures in the acinar, centro-acinar, intercalated portion and excretory duct cells. the islets of langerhans were found conspicuously enlarged due to m ...1976779717
the meadow vole as an experimental animal.a meadow vole colony has been in operation at the pennsylvania state university for 6 yr. the breeding colony consists of 16 breeding harems of 1 male and 2-4 females per cage. over 2,500 weanlings have been available each year for various experiments. primary emphasis has been directed toward determination of the weanling's nutritional requirements. adults have been used to study plant factors associated with the palatability of forages. this information has been used to devise specific bioassa ...1976787673
integrated pest management in the u.s.: progress and promise.in the u.s., where heavy use of insecticides has been commonplace for years, the development of proper integrated insect pest control cannot get underway unless there is a changed use pattern for such chemicals. a changed use pattern, however, cannot be accomplished without much study to establish the requirements for integrated control for each major crop situation. in this paper recent developments in a number of crop areas in the u.s. in which the necessary study has been begun are reviewed. ...1976789064
studies of folic acid compounds in nature. iv. folic acid compounds in soybeans and cow milk.folate compounds in soybean and cow's mild were identified by sephadex chromatography and differential microbiological assaymsoybean contained mainly monoglutamates (ca. 52%), some diglutamates (ca. 16%), and polyglutamates (pentaglutamates representing the major portion). 5-cho-h4pteglun(1-6) constituted 65-70% of total folate activity; cow's milk contained monoglutamates (60%) and polyglutamates (ranging from di- to hepta-conjugates). in contrast to soybean, 90-95% of milk folate was in the 5- ...1975804977
differences in the amount and range of volatile carbonyl compounds formed by lipoxygenase isoenzymes from soybeans. 2000806626
changes in the fluorescence and absorbance of lipoxygenase-1 induced by 13-ls-hydroperoxylinoleic acid and linoleic acid.1. the addition of 13-ls-hydroperoxylinoleic acid to lipoxygenase-1 (linoleate: oxygen oxidoreductase ec 1.13.11.12) from soybeans at ph 9 and 25 degrees c causes a quenching of the fluorescence of the enzyme at 328 nm when exciited at 280 nm and gives rise to an increase of the absorbance of the enzyme in the 300 nm to 450 nm region. 2. in the absence of 02, addition of linoleic acid to enzyme treated with 13-ls-hydroperoxylinoleic acid, causes an increase of the fluorescence at 328 nm and a de ...1975807258
the amino-acid sequence of leghemoglobin component a from phaseolus vulgaris (kidney bean).1. leghemoglobin component a from phaseolus vulgaris (kidney bean) was digested with trypsin; 15 tryptic peptides and free lysine were purified and the amino acid sequences of the peptides determined. 2. the internal order of the tryptic peptides was determined by the bridge peptides obtained from the thermolytic digest and the dilute acid hydrolyzate of kidney bean leghemoglobin a; 12 thermolytic peptides and two acid hydrolysis peptides were purified and the sequences were partially or complet ...1975809270
on the positional specificity of the oxygenation reaction catalysed by soybean lipoxygenase-1.lipoxygenase-1 from soybeans is incubated with an isomer of linoleic acid, 13-cis, 16-cis-octadecadienoic acid. analysis of the oxygenation products indicates that molecular oxygen is stereospecifically introduced mainly at c-17 (n-2) of the fatty acid (in the ls-configuration), and only to a minor extent at c-13 (n-6). these findings contradict previous suggestions about the postional specificity of lipoxygenase-1.1975812552
chemical composition and lipoxygenase activity in soybeans as affected by genotype and environment. 1976812898
discrimination of rhizobium japonicum, rhizobium lupini, rhizobium trifolii, rhizobium leguminosarum and of bacteroids by uptake of 2-ketoglutaric acid, glutamic acid and phosphate.rhizobium strains (one each of rh. japonicum, rh. lupini, rh. leguminosarum) take up 2-ketoglutaric acid in general much faster and from lower concentrations in the medium than strains of escherichia coli, bacillus subtilis and chromobacterium violaceum. a strain of enterobacter aerogenes, however, is more similar to some rhizobium strains. the same strains of rhizobium take up also phosphate much faster and from lower concentrations than the other bacteria tested. 4 strains of rh. lupini proved ...1976818969
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