| 16s ribosomal dna characterization of nitrogen-fixing bacteria isolated from banana (musa spp.) and pineapple (ananas comosus (l.) merril). | nitrogen-fixing bacteria isolated from banana (musa spp.) and pineapple (ananas comosus (l.) merril) were characterized by amplified 16s ribosomal dna restriction analysis and 16s rrna sequence analysis. herbaspirillum seropedicae, herbaspirillum rubrisubalbicans, burkholderia brasilensis, and burkholderia tropicalis were identified. eight other types were placed in close proximity to these genera and other alpha and beta proteobacteria. | 2001 | 11319127 |
| characterization of amplified polymerase chain reaction glnb and nifh gene fragments of nitrogen-fixing burkholderia species. | to clone and sequence polymerase chain reaction (pcr)-amplified glnb and nifh genes of the nitrogen-fixing bacteria burkholderia brasilensis strain m130, b. tropicalis strain ppe8 and b. kururiensis strain kp23. | 2003 | 12535125 |
| history on the biological nitrogen fixation research in graminaceous plants: special emphasis on the brazilian experience. | this review covers the history on biological nitrogen fixation (bnf) in graminaceous plants grown in brazil, and describes research progress made over the last 40 years, most of which was coordinated by johanna döbereiner. one notable accomplishment during this period was the discovery of several nitrogen-fixing bacteria such as the rhizospheric (beijerinckia fluminensis and azotobacter paspali), associative (azospirillum lipoferum, a. brasilense, a. amazonense) and the endophytic (herbaspirillu ... | 2005 | 16127558 |
| biodegradation of 2,4,6-trichlorophenol in a packed-bed biofilm reactor equipped with an internal net draft tube riser for aeration and liquid circulation. | for the aerobic biodegradation of the fungicide and defoliant 2,4,6-trichlorophenol (2,4,6-tcp), a bench-scale packed-bed bioreactor equipped with a net draft tube riser for liquid circulation and oxygenation (pb-alr) was constructed. to obtain a high packed-bed volume relative to the whole bioreactor volume, a high a(d)/a(r) ratio was used. reactor's downcomer was packed with a porous support of volcanic stone fragments. pb-alr hydrodynamics and oxygen mass transfer behavior was evaluated and c ... | 2009 | 18539387 |
| the new group of non-pathogenic plant-associated nitrogen-fixing burkholderia spp. shares a conserved quorum-sensing system, which is tightly regulated by the rsal repressor. | a novel group of nitrogen-fixing plant-associated burkholderia species has emerged in the last few years. the purpose of this investigation was to determine if these species possess an n-acylhomoserine lactone (ahl) quorum-sensing (qs) cell-cell signalling system, and whether it is important for nitrogen fixation and other phenotypic features in burkholderia kururiensis. it was determined that b. kururiensis, and other members of this burkholderia species cluster, contain at least one highly con ... | 2008 | 18599833 |
| potential plant growth promoting traits and bioacidulation of rock phosphate by thiosulfate oxidizing bacteria isolated from crop plants. | thiosulfate oxidizing bacteria isolated from crop plants were tested for their traits related to plant growth promotion and their ability to solubilize morocco rock phosphate (rp) through oxidation of thiosulfate to sulfuric acid. all the tested strains grew in nfb medium (except dyella ginsengisoli) and possessed beta-1,3 glucanase activity (except burkholderia kururiensis). of the fourteen tested strains, 2 were found positive for siderophore production, 3 each for phytohormones (iaa), and sal ... | 2008 | 18785656 |
| endophytic colonization of rice (oryza sativa l.) by the diazotrophic bacterium burkholderia kururiensis and its ability to enhance plant growth. | burkholderia kururiensis is a diazotrophic bacterium originally isolated from a polluted aquifer environment and presents a high level of similarity with the rice endophyte "b. brasilensis" species. this work assessed the ability of b. kururiensis to endophytically colonize rice plantlets by monitoring different tissues of root-inoculated plants for the presence of bacterial growth in different media, electron microscopy and by 16s rdna analysis. observations of roots, stems and leaves of inocul ... | 2008 | 18797800 |
| mixotrophic metabolism in burkholderia kururiensis subsp. thiooxydans subsp. nov., a facultative chemolithoautotrophic thiosulfate oxidizing bacterium isolated from rhizosphere soil and proposal for classification of the type strain of burkholderia kururiensis as burkholderia kururiensis subsp. kururiensis subsp. nov. | a thiosulfate-oxidizing facultative chemolithoautotrophic burkholderia sp. strain atsb13(t) was previously isolated from rhizosphere soil of tobacco plant. strain atsb13(t) was aerobic, gram-staining-negative, rod shaped and motile by means of sub-terminal flagellum. strain atsb13(t) exhibited mixotrophic growth in a medium containing thiosulfate plus acetate. a phylogenetic study based on 16s rrna gene sequence analysis indicated that strain atsb13(t) was most closely related to burkholderia ku ... | 2009 | 19841903 |
| rapid identification of nitrogen-fixing and legume-nodulating burkholderia species based on pcr 16s rrna species-specific oligonucleotides. | several novel n(2)-fixing burkholderia species associated with plants, including legume-nodulating species, have recently been discovered. presently, considerable interest exists in studying the diazotrophic burkholderia species, both for their ecology and their great potential for agro-biotechnological applications. however, the available methods used in the identification of these burkholderia species are time-consuming and expensive. in this study, pcr species-specific primers based on the 16 ... | 2010 | 19945811 |
| commonalities and differences in regulation of n-acyl homoserine lactone quorum sensing in the beneficial plant-associated burkholderia species cluster. | the genus burkholderia includes over 60 species isolated from a wide range of environmental niches and can be tentatively divided into two major species clusters. the first cluster includes pathogens such as burkholderia glumae, b. pseudomallei, and b. mallei and 17 well-studied species of the burkholderia cepacia complex. the other recently established cluster comprises at least 29 nonpathogenic species, which in most cases have been found to be associated with plants. it was previously establi ... | 2010 | 20435760 |
| rice root-associated bacteria: insights into community structures across 10 cultivars. | in this study, the effects of plant genotype, soil type and nutrient use efficiency on the composition of different bacterial communities associated with rice roots were investigated. thus, total bacteria, alpha- and betaproteobacteria, pseudomonas and actinobacteria were studied using pcr, followed by denaturing gradient gel electrophoresis (pcr-dgge). rice genotype determined, to a large extent, the composition of the different bacterial communities across cultivars. several cultivars belongin ... | 2011 | 21426364 |
| burkholderia symbiotica sp. nov., isolated from root nodules of mimosa spp. native to north east brazil. | four strains, jpy345t, jpy347, jpy366 and jpy581 were isolated from nitrogen-fixing nodules on the roots of two mimosa spp. (m. cordistipula, m. misera) native to north east brazil, and their taxonomic positions were investigated by a polyphasic approach. all four strains grew at 15-43 °c (optimum, 35 °c), at ph 4.0-7.0 (optimum, ph 5.0) and with 0-2 % nacl (optimum, 0 %). on the basis of 16s rrna gene sequence analysis, a representative strain (jpy345t) showed 97.3 % sequence similarity to t ... | 2011 | 22081715 |
| brassica napus hairy roots and rhizobacteria for phenolic compounds removal. | phenolic compounds are contaminants frequently found in water and soils. in the last years, some technologies such as phytoremediation have emerged to remediate contaminated sites. plants alone are unable to completely degrade some pollutants; therefore, their association with rhizospheric bacteria has been proposed to increase phytoremediation potential, an approach called rhizoremediation. in this work, the ability of two rhizobacteria, burkholderia kururiensis kp 23 and agrobacterium rhizogen ... | 2013 | 22961561 |
| degradation of multiwall carbon nanotubes by bacteria. | understanding the environmental transformation of multiwall carbon nanotubes (mwcnts) is important to their life cycle assessment and potential environmental impacts. we report that a bacterial community is capable of degrading (14)c-labeled mwcnts into (14)co2 in the presence of an external carbon source via co-metabolism. multiple intermediate products were detected, and genotypic characterization revealed three possible microbial degraders: burkholderia kururiensis, delftia acidovorans, and s ... | 2013 | 23859846 |
| plant-influenced gene expression in the rice endophyte burkholderia kururiensis m130. | burkholderia kururiensis m130 is one of the few rice endophytic diazotrophic bacteria identified thus far which is able to enhance growth of rice. to date, very little is known of how strain m130 and other endophytes enter and colonize plants. here, we identified genes of strain m130 that are differentially regulated in the presence of rice plant extract. a genetic screening of a promoter probe transposon mutant genome bank and rnaseq analysis were performed. the screening of 10,100 insertions o ... | 2015 | 25494355 |
| burkholderia caballeronis sp. nov., a nitrogen fixing species isolated from tomato (lycopersicon esculentum) with the ability to effectively nodulate phaseolus vulgaris. | during a survey of burkholderia species with potential use in agrobiotechnology, a group of 12 strains was isolated from the rhizosphere and rhizoplane of tomato plants growing in mexico (nepantla, mexico state). a phylogenetic analysis of 16s rrna gene sequences showed that the strains are related to burkholderia kururiensis and burkholderia mimosarum (97.4 and 97.1 %, respectively). however, they induced effective nitrogen-fixing nodules on roots of phaseolus vulgaris. based on polyphasic taxo ... | 2013 | 24057982 |
| degradation of benzene, toluene, and xylene isomers by a bacterial consortium obtained from rhizosphere soil of cyperus sp. grown in a petroleum-contaminated area. | increasing contamination of soil and groundwater with benzene, toluene, and xylene (btx) due to activities of the chemical and oil refinery industry has caused serious environmental damage. efficient methods are required to isolate and degrade them. microorganisms associated with rhizosphere soil are considered efficient agents to remediate hydrocarbon contamination. in this study, we obtained a stabilized bacterial consortium from the rhizosphere soil of cyperus sp. grown in a petroleum-contami ... | 2013 | 23564628 |
| draft genome sequence of the rice endophyte burkholderia kururiensis m130. | burkholderia kururiensis m130 is one of the few characterized rice endophytes and was isolated from surface-sterilized rice roots. this bacterium shows strong growth-promoting effects, being able to increase rice yields. here we present its draft genome sequence, which contains important traits for endophytic life and plant growth promotion. | 2013 | 23558537 |
| characterization of rhamnolipids produced by wild-type and engineered burkholderia kururiensis. | biosurfactants are a class of functional molecules produced and secreted by microorganisms, which play important roles in cell physiology such as flagellum-dependent or -independent bacterial spreading, cell signaling, and biofilm formation. they are amphipathic compounds and comprise a variety of chemical structures, including rhamnolipids, typically produced by pseudomonas spp. and also reported within other bacterial genera. the present study is focused on burkholderia kururiensis kp23(t), a ... | 2013 | 23053103 |
| effects of elevated carbon dioxide, elevated temperature, and rice growth stage on the community structure of rice root-associated bacteria. | the effects of free-air carbon dioxide enrichment (face) and elevated soil and water temperature (warming) on the rice root-associated bacterial community were evaluated by clone library analysis of the 16s ribosomal rna gene. roots were sampled at the panicle initiation and ripening stages 41 and 92 days after transplanting (dat), respectively. the relative abundances of the methanotrophs methylosinus and methylocystis were increased by warming and decreased by face at 92 dat, which indicated t ... | 2014 | 24882221 |
| structural elucidation of the repeat unit in highly branched acidic exopolysaccharides produced by nitrogen fixing burkholderia. | burkholderia kururiensis, strain m130, an endophytic diazotrophic bacterium isolated from rice roots, produces acetylated acidic exopolysaccharides which can be separated by anion exchange chromatography. these were characterized by nuclear magnetic resonance spectroscopy, methylation analysis and smith degradation. the exopolysaccharides eluted with 0.5 m nacl were produced when the bacterium was grown in a medium containing mannitol as the sole carbon source, and showed to be a mixture of two ... | 2010 | 19933228 |
| burkholderia acidipaludis sp. nov., aluminum-tolerant bacteria isolated from chinese water chestnut (eleocharis dulcis) growing in highly acidic swamps in south-east asia. | two strains of aluminium-tolerant bacteria, sa33(t) and 7a078, were isolated from chinese water chestnut (eleocharis dulcis) growing in highly acidic swamps (ph 2-4) in actual acid sulfate soil areas of vietnam (sa33(t)) and thailand (7a078). the strains were gram-negative, aerobic, non-spore-forming rods, 0.6-0.7 mum wide and 1.3-1.7 mum long. these strains showed good growth at ph 3.0-8.0 and 17-37 degrees c. the organisms contained ubiquinone q-8 as the predominant isoprenoid quinone and c(16 ... | 2010 | 19819996 |
| burkholderia kururiensis sp. nov., a trichloroethylene (tce)-degrading bacterium isolated from an aquifer polluted with tce. | a trichloroethylene (tce)-degrading bacterium was isolated from an aquifer sample collected at a tce-polluted site in japan by enriching with phenol as sole carbon source. the isolate, designated strain kp23t, was a gram-negative, oval-shaped micro-organism. a phylogenetic study based on 16s rrna gene sequences indicated that strain kp23t should be placed in the genus burkholderia. cellular fatty acids of the strain were mainly composed of c16:0, cyclopropanic acid c17:0 and cyclopropanic acid c ... | 2000 | 10758884 |