Publications

study of betasatellite molecule from leaf curl disease of sunn hemp (crotalaria juncea) in india.	leaves of sunn hemp (crotalaria juncea) showing geminiviral symptoms were collected from lucknow, india during rainy season in 2008. dna template isolated from the symptomatic leaf tissues were subjected to polymerase chain reaction (pcr) using specific primers to amplify coat protein (cp) gene of dna-a as well as betasatellite dna associated with the leaf curl disease. cp gene showed 97% sequence identity with that of cotton leaf curl burewala virus (clcubwv). further, the betasatellite dna mol ...	2010	20890652
cotton leaf curl disease in resistant cotton is associated with a single begomovirus that lacks an intact transcriptional activator protein.	cotton leaf curl disease (clcud) is the major limitation to cotton production across pakistan and northwestern india. the disease first appeared in epidemic form in the 1980s and was shown to be caused by monopartite begomoviruses (seven distinct species have thus far been shown to be involved), frequently as multiple infections. additionally, the viruses are associated with a specific satellite, the clcud betasatellite cotton leaf curl multan betasatellite (clcumb), which is responsible for the ...	2010	20600387
xanthium strumarium: a weed host of components of begomovirus-betasatellite complexes affecting crops.	xanthium strumarium is a common weed that often shows symptoms typical of begomovirus infection, such as leaf curling and vein thickening. the virus complex isolated from the weed consisted of two begomoviruses along with a betasatellite and an alphasatellite. the first begomovirus was shown to be an isolate of cotton leaf curl burewala virus, a new recombinant begomovirus species that is associated with resistance breaking in previously resistant cotton varieties in pakistan, whereas the second ...	2011	21969121
dominance of resistance-breaking cotton leaf curl burewala virus (clcubuv) in northwestern india.	cotton leaf curl disease (clcud) is a major limitation to cotton production on the indian subcontinent. a survey for viruses causing clcud was conducted during the 2009 and 2010 cropping seasons in the northwestern indian cotton-growing belt in the states of punjab, haryana and rajasthan. partial sequences of 258 and full-length sequences of 22 virus genomes were determined. this study shows that the resistance-breaking cotton leaf curl burewala virus (clcubuv) is now the dominant virus in many ...	2012	22307170
transcript mapping of cotton leaf curl burewala virus and its cognate betasatellite, cotton leaf curl multan betasatellite.	whitefly-transmitted geminiviruses (family geminiviridae, genus begomovirus) are major limiting factors for the production of numerous dicotyledonous crops throughout the warmer regions of the world. in the old world a small number of begomoviruses have genomes consisting of two components whereas the majority have single-component genomes. most of the monopartite begomoviruses associate with satellite dna molecules, the most important of which are the betasatellites. cotton leaf curl disease (c ...	2012	23106938
artificial microrna-mediated resistance against the monopartite begomovirus cotton leaf curl burewala virus.	cotton leaf curl disease, caused by single-stranded dna viruses of the genus begomovirus (family geminiviridae), is a major constraint to cotton cultivation across pakistan and north-western india. at this time only cotton varieties with moderate tolerance are available to counter the disease. micrornas (mirnas) are a class of endogenous small rna molecules that play an important role in plant development, signal transduction, and response to biotic and a biotic stress. studies have shown that m ...	2013	23844988
a new betasatellite associated with cotton leaf curl burewala virus infecting tomato in india: influence on symptoms and viral accumulation.	a begomovirus and its associated alpha- and betasatellite were detected in tomato plants affected with leaf curl disease. based on a nucleotide sequence identity of 99 %, this begomovirus was designated an isolate of cotton leaf curl burewala virus (clcubuv). the alphasatellite exhibited 93 % sequence identity to cotton leaf curl burewala alphasatellite (clcubua) and is hence referred to here as a variant of clcubua. the detected betasatellite was recombinant in nature and showed 70 % sequence i ...	2013	23361863
a distinct strain of chickpea chlorotic dwarf virus (genus mastrevirus, family geminiviridae) identified in cotton plants affected by leaf curl disease.	as part of a study to determine the diversity of whitefly-transmitted viruses (genus begomovirus, family geminiviridae) associated with cotton leaf curl disease in pakistan, leaf samples from cotton plants showing typical leaf curl disease symptoms were collected in various locations of punjab province. sequence analysis of full-length virus clones (~2.7 kb) showed plants to be infected with the begomovirus cotton leaf curl burewala virus, the only virus identified in cotton in the punjab since ...	2014	24212888
functional characterization of a bidirectional plant promoter from cotton leaf curl burewala virus using an agrobacterium-mediated transient assay.	the c1 promoter expressing the ac1 gene, and v1 promoter expressing the av1 gene are located in opposite orientations in the large intergenic region of the cotton leaf curl burewala virus (clcubuv) genome. agro-infiltration was used to transiently express putative promoter constructs in nicotiana tabacum and gossypium hirsutum leaves, which was monitored by a gus reporter gene, and revealed that the bidirectional promoter of clcubuv transcriptionally regulates both the ac1 and av1 genes. the clc ...	2014	24424501
effects of genetic changes to the begomovirus/betasatellite complex causing cotton leaf curl disease in south asia post-resistance breaking.	cotton leaf curl disease (clcud) has been a problem for cotton production across pakistan and north-eastern india since the early 1990s. the appearance of the disease has been attributed to the introduction, and near monoculture of highly susceptible cotton varieties. during the intervening period the genetic make-up of the virus(es) causing the disease has changed dramatically. the most prominent of these changes has been in response to the introduction of clcud-resistant cotton varieties in th ...	2014	24361351
identification of a distinct strain of cotton leaf curl burewala virus.	cotton leaf curl disease (clcud) is the major biotic limitation to cotton production in pakistan and northwestern india. the disease is caused by various distinct viruses of the genus begomovirus (family geminiviridae) in association with a disease-specific betasatellite - cotton leaf curl multan betasatellite (clcumb). since 2001, when resistance to clcud in cotton was broken, only one virus was consistently identified in cotton exhibiting clcud symptoms in punjab province (pakistan) - cotton l ...	2014	24838851
cotton leaf curl burewala virus with intact or mutant transcriptional activator proteins: complexity of cotton leaf curl disease.	cotton leaf curl disease (clcud) is a serious disease of cotton on the indian subcontinent. in the present study, three cotton leaf curl viruses, cotton leaf curl burewala virus (clcubuv), cotton leaf curl kokhran virus (clcukov) and cotton leaf curl multan virus (clcumv), and their associated satellites, cotton leaf curl multan betasatellite (clcumb) and cotton leaf curl multan alphasatellite (clcuma), were detected. clcubuv with either intact (clcubuv-1) or mutant (clcubuv-2) transcriptional a ...	2015	25772572
functional characterization of a strong bi-directional constitutive plant promoter isolated from cotton leaf curl burewala virus.	cotton leaf curl burewala virus (clcubuv), belonging to the genus begomovirus, possesses single-stranded monopartite dna genome. the bidirectional promoters representing rep and coat protein (cp) genes of clcubuv were characterized and their efficacy was assayed. rep and cp promoters of clcubuv and 35s promoter of cauliflower mosaic virus (camv) were fused with β-glucuronidase (gus) and green fluorescent protein (gfp) reporter genes. gus activity in individual plant cells driven by rep, cp and 3 ...	2015	25799504