| ranavirus phylogenomics: signatures of recombination and inversions among bullfrog ranaculture isolates. | ranaviruses are emerging pathogens of fish, amphibians, and reptiles that threaten aquatic animal industries and wildlife worldwide. our objective was to genetically characterize ranaviruses isolated during separate bullfrog lithobates catesbeianus die-offs that occurred eight years apart on the same north american farm. the earlier outbreak was due to a highly pathogenic strain of common midwife toad virus (cmtv) previously known only from europe and china. the later outbreak was due to a chime ... | 2017 | 28803676 |
| screening of a long-term sample set reveals two ranavirus lineages in british herpetofauna. | reports of severe disease outbreaks in amphibian communities in mainland europe due to strains of the common midwife toad virus (cmtv)-like clade of ranavirus are increasing and have created concern due to their considerable population impacts. in great britain, viruses in another clade of ranavirus-frog virus 3 (fv3)-like-have caused marked declines of common frog (rana temporaria) populations following likely recent virus introductions. the british public has been reporting mortality incidents ... | 2017 | 28931029 |
| genomic sequencing of ranaviruses isolated from edible frogs (pelophylax esculentus). | ranaviruses were isolated from wild edible frogs (pelophylax esculentus) during epizootics in denmark and italy. phylogenomic analyses revealed that these isolates are closely related and belong to a clade of ranaviruses that includes the andrias davidianus ranavirus (adrv), common midwife toad ranavirus (cmtv), testudo hermanni ranavirus (thrv), and pike-perch iridovirus (ppiv). | 2017 | 28935748 |
| the genome sequence of the emerging common midwife toad virus identifies an evolutionary intermediate within ranaviruses. | worldwide amphibian population declines have been ascribed to global warming, increasing pollution levels, and other factors directly related to human activities. these factors may additionally be favoring the emergence of novel pathogens. in this report, we have determined the complete genome sequence of the emerging common midwife toad ranavirus (cmtv), which has caused fatal disease in several amphibian species across europe. phylogenetic and gene content analyses of the first complete genomi ... | 2012 | 22301140 |
| virion-associated viral proteins of a chinese giant salamander (andrias davidianus) iridovirus (genus ranavirus) and functional study of the major capsid protein (mcp). | chinese giant salamander iridovirus (cgsiv) is the emerging causative agent to farmed chinese giant salamanders in nationwide china. cgsiv is a member of the common midwife toad ranavirus (cmtv) subset of the amphibian-like ranavirus (alrv) in the genus ranavirus of iridoviridae family. however, viral protein information on alrv is lacking. in this first proteomic analysis of alrv, 40 cgsiv viral proteins were detected from purified virus particles by liquid chromatography-tandem mass spectromet ... | 2014 | 24906872 |
| complete genome sequence of a common midwife toad virus-like ranavirus associated with mass mortalities in wild amphibians in the netherlands. | a ranavirus associated with mass mortalities in wild water frogs (pelophylax spp.) and other amphibians in the netherlands since 2010 was isolated, and its complete genome sequence was determined. the virus has a genome of 107,772 bp and shows 96.5% sequence identity with the common midwife toad virus from spain. | 2014 | 25540340 |
| investigation of amphibian mortality events in wildlife reveals an on-going ranavirus epidemic in the north of the netherlands. | in the four years following the first detection of ranavirus (genus ranavirus, family iridoviridae) infection in dutch wildlife in 2010, amphibian mortality events were investigated nationwide to detect, characterize and map ranaviruses in amphibians over time, and to establish the affected host species and the clinico-pathological presentation of the disease in these hosts. the ultimate goal was to obtain more insight into ranavirus disease emergence and ecological risk. in total 155 dead amphi ... | 2016 | 27315226 |
| monitoring ranavirus-associated mortality in a dutch heathland in the aftermath of a ranavirus disease outbreak. | the ranaviruses are an emerging group of viruses that infect amphibians, fish, and reptiles. although ranaviruses have not been linked to extinctions, emergence in amphibian communities has resulted in population declines for some species. we present the results of ranavirus-associated mortality in a dutch national park in the aftermath of an outbreak associated with a common midwife toad virus (cmtv)-like ranavirus. we monitored five bodies of water across dwingelderveld national park, the neth ... | 2016 | 27455198 |
| comparative genomics of amphibian-like ranaviruses, nucleocytoplasmic large dna viruses of poikilotherms. | recent research on genome evolution of large dna viruses has highlighted a number of incredibly dynamic processes that can facilitate rapid adaptation. the genomes of amphibian-like ranaviruses - double-stranded dna viruses infecting amphibians, reptiles, and fish (family iridoviridae) - were examined to assess variation in genome content and evolutionary processes. the viruses studied were closely related, but their genome content varied considerably, with 29 genes identified that were not pres ... | 2015 | 27812275 |
| concurrent ranavirus and batrachochytrium dendrobatidis infection in captive frogs (phyllobates and dendrobates species), the netherlands, 2012: a first report. | a ranavirus infection with concurrent batrachochytrium dendrobatidis infection and mortality in captive phyllobates and dendrobates species is reported. greyish skin with hepato- and reno-megaly were evident. microscopically, batrachochytrium dendrobatidis was present in the stratum corneum of the hyperkeratotic skin. intracytoplasmic inclusion bodies were present in erythrocytes and multiple organs. all samples examined tested positive using pcr for the major capsid protein (mcp) gene of ranavi ... | 2012 | 23102620 |
| ranavirus causes mass die-offs of alpine amphibians in the southwestern alps, france. | pathogenic fungi and viruses cause mortality outbreaks in wild amphibians worldwide. in the summer of 2012, dead tadpoles and adults of the european common frog rana temporaria were reported in alpine lakes in the southwestern alps (mercantour national park, france). a preliminary investigation using molecular diagnostic techniques identified a ranavirus as the potential pathogenic agent. three mortality events were recorded in the park, and samples were collected. the amphibian chytrid fungus b ... | 2016 | 26967128 |
| selected emerging diseases of amphibia. | this review summarizes the most recent updates on emerging infectious diseases of amphibia. a brief summary of batrachochytrium dendrobatidis history, epidemiology, pathogenesis, life cycle, diagnosis, treatment, and biosecurity is provided. ambystoma tigrinum virus, common midwife toad virus, frog virus 3, rana grylio virus, rana catesbeiana ranavirus, mahaffey road virus, rana esculenta virus, bohle iridovirus, and tiger frog virus ranaviruses are extensively reviewed. emerging bacterial patho ... | 2013 | 23642863 |
| ranavirus-associated mass mortality in wild amphibians, the netherlands, 2010: a first report. | in 2010, a mass die-off of over 1000 wild water frogs (pelophylax spp.) and at least 10 common newts (lissotriton vulgaris) occurred in a pond in the netherlands. haemorrhagic disease with hepatomegaly and splenomegaly was evident. microscopically, multiple organs presented cells with multifocal intracytoplasmic inclusion bodies, in which ranavirus-like particles were demonstrated ultrastructurally. all specimens examined tested positive for ranavirus by pcr. the sequence obtained showed a 100% ... | 2011 | 21955440 |
| outbreak of common midwife toad virus in alpine newts (mesotriton alpestris cyreni) and common midwife toads (alytes obstetricans) in northern spain: a comparative pathological study of an emerging ranavirus. | this report describes the isolation and characterisation of the common midwife toad virus (cmtv) from juvenile alpine newts (mesotriton alpestris cyreni) and common midwife toad (cmt) tadpoles (alytes obstetricans) in the picos de europa national park in northern spain in august 2008. a comparative pathological and immunohistochemical study was carried out using anti-cmtv polyclonal serum. in the kidneys, glomeruli had the most severe histological lesions in cmt tadpoles, while both glomeruli an ... | 2010 | 19703784 |
| pathology, isolation and molecular characterisation of a ranavirus from the common midwife toad alytes obstetricans on the iberian peninsula. | we describe the pathology, isolation and characterisation of a virus responsible for an outbreak of a systemic haemorrhagic disease causing high mortality in tadpoles of the common midwife toad alytes obstetricans in the 'picos de europa' national park in northern spain. the virus, provisionally designated as the common midwife toad virus (cmtv), was isolated from homogenates of visceral tissue from diseased toad tadpoles following inoculation on epithelioma papulosum cyprini (epc) cells. molecu ... | 2009 | 19476279 |