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effect of various factors on the activity of trehalase from the larvae of sesamia inferens walker (insect).trehalase from the salivary glands and the midgut of sesamia inferens showed optimum activity at ph 5.8, and at temperatures of 50 and 60 degrees c respectively. the increase in the incubation period, enzyme concentration, and substrate concentration respectively increased the end-product, the hydrolysis, and the rate of hydrolysis of the substrate. dialysis did not affect, tryptophan accelerated, and other amino acids and end-product inhibited the enzyme activity.200014845
comparative venom toxicity between pteromalus puparum and nasonia vitripennis (hymenoptera: pteromalidae) toward the hemocytes of their natural hosts, non-target insects and cultured insect cells.crude venoms from two parasitoid species, pteromalus puparum and nasonia vitripennis (hymenoptera: pteromalidae) were assayed for biological activities toward hemocytes from two species of their natural hosts and eight species of their non-natural hosts as well as two lines of cultured lepidoptera cells, respectively. by inhibiting the spreading and viability of insect hemocytes, the venom from p. puparum displayed significantly higher activities toward plasmatocytes and granular cells from both ...200516026808
do rice water weevils and rice stem borers compete when sharing a host plant?the rice water weevil (rww) lissorhoptrus oryzophilus kuschel (coleoptera: curculionidae) is an invasive insect pest of rice oryza sativa l. in china. little is known about the interactions of this weevil with indigenous herbivores. in the present study, adult feeding and population density of the weevil, injury level of striped stem borer chilo suppressalis (walker) (lepidoptera: pyralidae) and pink stem borer sesamia inferens (walker) (lepidoptera: noctuidae) to rice, as well as growth status ...200818600788
differential fipronil susceptibility and metabolism in two rice stem borers from china.the susceptibilities of larvae of two rice stem borers, namely, chilo suppressalis (walker) (lepidoptera: crambidae) and sesamia inferens (walker) (lepidoptera: nocutidae) to fipronil and its metabolites were investigated, and then the activities of microsomal o-demethylase, and glutathione transferase (gst) in two species were measured. the metabolism of fipronil in both stem borers was determined in vivo and in vitro. the ld50 value of fipronil to s. inferens was 118.5-fold higher than that of ...200818767755
population dynamics of sesamia inferens on transgenic rice expressing cry1ac and cpti in southern china.genetically modified insect-resistant rice lines containing the cry1ac gene from bacillus thuringiensis (bt) or the cpti (cowpea trypsin inhibitor) gene developed for the management of lepidopterous pests are highly resistant to the major target pests, chilo suppressalis (walker), cnaphalocrocis medinalis (guenée), and scirpophaga incertulas (walker), in the main rice-growing areas of china. however, the effects of these transgenic lines on sesamia inferens (walker), an important lepidopterous r ...200819036217
screen of bacillus thuringiensis toxins for transgenic rice to control sesamia inferens and chilo suppressalis.transgenic rice to control stem borer damage is under development in china. to assess the potential of bacillus thuringiensis (bt) transgenes in stem borer control, the toxicity of five bt protoxins (cry1aa, cry1ab, cry1ac, cry1ba and cry1ca) against two rice stem borers, sesamia inferens (pink stem borer) and chilo suppressalis (striped stem borer), was evaluated in the laboratory by feeding neonate larvae on artificial diets containing bt protoxins. the results indicated that cry1ca exhibited ...201020452358
baseline sensitivity of lepidopteran corn pests in india to cry1ab insecticidal protein of bacillus thuringiensis.genetically engineered corn (bt corn) expressing bacillus thuringiensis berliner insecticidal protein cry1ab is a biotechnological option being considered for management of lepidopteran corn pests in india. as a resistance management practice it was essential to determine the sensitivity of multiple populations of the stalk borer chilo partellus (swinhoe), pink borer sesamia inferens (walker) and the cob borer helicoverpa armigera (hübner) to cry1ab protein through bioassays. the insect populati ...201020602522
transgenic rice plants expressing a fused protein of cry1ab/vip3h has resistance to rice stem borers under laboratory and field conditions.six transgenic rice, oryza sativa l., lines (g6h1, g6h2, g6h3, g6h4, g6h5, and g6h6) expressing a fused cry1ab/vip3h protein, were evaluated for resistance against the asiatic rice borer, chilo suppressalis (walker) (lepidoptera: crambidae), and the stem borer sesamia inferens (walker) (lepidoptera: noctuidae) in the laboratory and field. the bioassay results indicated that the mortality of asiatic rice borer and s. inferens neonate larvae on six transgenic lines from seedling to filling stage w ...201020857760
evaluating the non-rice host plant species of sesamia inferens (lepidoptera: noctuidae) as natural refuges: resistance management of bt rice.although rice (oryza sativa l.) lines that express bacillus thuringiensis (bt) toxins have shown great potential for managing the major lepidoptera pests of rice in southern china, including sesamia inferens, their long-term use is dependent on managing resistance development to bt toxins in pest populations. the maintenance of "natural" refuges, non-bt expressing plants that are hosts for a target pest, has been proposed as a means to minimize the evolution of resistance to bt toxins in transge ...201122251655
the complete mitochondrial genome of the rice moth, corcyra cephalonica.the complete mitochondrial genome (mitogenome) of the rice moth, corcyra cephalonica stainton (lepidoptera: pyralidae) was determined as a circular molecular of 15,273 bp in size. the mitogenome composition (37 genes) and gene order are the same as the other lepidopterans. nucleotide composition of the c. cephalonica mitogenome is highly a+t biased (80.43%) like other insects. twelve protein-coding genes start with a typical atn codon, with the exception of coxl gene, which uses cga as the initi ...201223413968
differential expression patterns in chemosensory and non-chemosensory tissues of putative chemosensory genes identified by transcriptome analysis of insect pest the purple stem borer sesamia inferens (walker).a large number of insect chemosensory genes from different gene subfamilies have been identified and annotated, but their functional diversity and complexity are largely unknown. a systemic examination of expression patterns in chemosensory organs could provide important information.201323894529
antenna-predominant and male-biased csp19 of sesamia inferens is able to bind the female sex pheromones and host plant volatiles.insect chemosensory proteins (csps) are proposed to capture and transport hydrophobic chemicals across the sensillum lymph to olfactory receptors (ors), but this has not been clarified in moths. in this study, we built on our previously reported segment sequence work and cloned the full length csp19 gene (sinfcsp19) from the antennae of sesamia inferens by using rapid amplification of cdna ends. quantitative real time-pcr (qpcr) assays indicated that the gene was expressed in a unique profile, i ...201424361960
binding site concentration explains the differential susceptibility of chilo suppressalis and sesamia inferens to cry1a-producing rice.chilo suppressalis and sesamia inferens are two important lepidopteran rice pests that occur concurrently during outbreaks in paddy fields in the main rice-growing areas of china. previous and current field tests demonstrate that the transgenic rice line huahui 1 (hh1) producing a cry1ab-cry1ac hybrid toxin from the bacterium bacillus thuringiensis reduces egg and larval densities of c. suppressalis but not of s. inferens. this differential susceptibility to hh1 rice correlates with the reduced ...201424928872
antennal transcriptome analysis and comparison of olfactory genes in two sympatric defoliators, dendrolimus houi and dendrolimus kikuchii (lepidoptera: lasiocampidae).the yunnan pine and simao pine caterpillar moths, dendrolimus houi lajonquière and dendrolimus kikuchii matsumura (lepidoptera: lasiocampidae), are two closely related and sympatric pests of coniferous forests in southwestern china, and olfactory communication systems of these two insects have received considerable attention because of their economic importance. however, there is little information on the molecular aspect of odor detection about these insects. furthermore, although lepidopteran ...201424998398
characterization of the complete mitochondrial genome of chilo auricilius and comparison with three other rice stem borers.the mitogenome of chilo auricilius (lepidoptera: pyraloidea: crambidae) was a circular molecule made up of 15,367 bp. sesamia inferens, chilo suppressalis, tryporyza incertulas, and c. auricilius, are closely related, well known rice stem borers that are widely distributed in the main rice-growing regions of china. the gene order and orientation of all four stem borers were similar to that of other insect mitogenomes. among the four stem borers, all at contents were below 83%, while all at conte ...201425042162
baseline sensitivity of maize borers in india to the bacillus thuringiensis insecticidal proteins cry1a.105 and cry2ab2.among the major pests of maize in india are two stem borers, chilo partellus (swinhoe) and sesamia inferens (walker), and an earworm, helicoverpa armigera (hübner). as a pest control strategy, transgenic bacillus thuringiensis (bt) maize hybrids are undergoing regulatory trials in india. we have determined the sensitivity of the target lepidopterans to the insecticidal bt proteins expressed in bt maize, as this determines product efficacy and the resistance management strategy to be adopted. mai ...201525143318
a 90-day subchronic feeding study of genetically modified rice expressing cry1ab protein in sprague-dawley rats.bacillus thuringiensis (bt) transgenic rice line (mfb-mh86) expressing a synthetic cry1ab gene can be protected against feeding damage from lepidopteran insects, including sesamia inferens, chilo suppressalis, tryporyza incertulas and cnaphalocrocis medinalis. rice flour from mfb-mh86 and its near-isogenic control mh86 was separately formulated into rodent diets at concentrations of 17.5, 35 and 70 % (w/w) for a 90-day feeding test with rats, and all of the diets were nutritionally balanced. in ...201525367203
chilo suppressalis and sesamia inferens display different susceptibility responses to cry1a insecticidal proteins.chilo suppressalis and sesamia inferens are important lepidopteran rice pests that occur concurrently in rice-growing areas of china. the development of transgenic rice expressing cry1a insecticidal proteins has provided a useful strategy for controlling these pests.201525469810
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