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a randomized, pilot trial comparing full versus escalating dose regimens for the desensitization of aids patients allergic to sulfonamides.sulfonamides are drugs extensively used in the management of aids patients. however, the use of sulfonamides is often associated with the development of allergic reactions, provoking the substitution of the drug (by another that may be less effective); alternatively attempts are made to desensitize the patient.200312585970
[human intestinal coccidiosis caused by isospora belli]. 195813585037
hiv1 and the gut in the era of highly active antiretroviral therapy.the gut and its gut-associated lymphoid tissue serve as a preferential site for hiv1 entry, active viral replication, reservoir, and hiv-mediated cd4 cell apoptosis. the widespread use of highly active antiretroviral therapy (haart) has resulted in a significant decrease in the incidence of opportunistic enteric pathogens as a consequence of immune recovery. nonetheless, patients with advanced hiv1 disease who were recently diagnosed or have poor response to haart can still suffer from opportuni ...200212228041
effect of antiretroviral therapy on cryptosporidiosis and microsporidiosis in patients infected with human immunodeficiency virus type 1.to better understand whether potent antiretroviral therapies can modify the natural history of hiv-1-associated microsporidiosis and cryptosporidiosis, the response to antimicrobial treatment of these opportunistic infections was evaluated in patients with or without antiretroviral treatment. fifty patients with diarrhoea, all positive for cryptosporidium parvum or enterocytozoon bieneusi, were included in the study. retrospective data were collected concerning demographics, clinical and microbi ...200010795595
experimental human coccidial enteritis by isospora belli wenyon, 1923. a study based on the infection of 5 volunteers. 196213944889
emerging pathogens: isospora, cyclospora and microsporidia.isospora belli, cyclospora cayetanensis as well as several species of microsporidia are recognized as emerging protozoan pathogens of humans. all are obligate intracellular parasites, with isospora and the microsporidia being primarily associated with immunocompromised hosts. cyclospora is a cause of traveller's diarrhoea, and is responsible for water-borne and food-borne outbreaks of disease. drug treatment is available for these infections. improved diagnostic methods including the autofluores ...199810660937
role of coccidian parasites in causation of diarrhoea in hiv infected patients in chennai.diarrhoea is one of the major problems of hiv positive patients. a coproparasitological study was carried out to assess the role of coccidian parasites in the causation of diarrhoea in hiv infected patients in chennai.200212619445
taxonomic position of the human intestinal protozoan parasite isospora belli as based on ribosomal rna sequences.the taxonomic positions of isospora belli and other members of the genus isospora are controversial. we determined the small-subunit ribosomal rna of i. belli and used this sequence in combination with other coccidian rna sequences for analysis of the taxonomic position of i. belli. the phylogenetic trees we obtained provide molecular evidence for three clades within a monophyletic group that represents the suborder eimeriina. the clade containing i. belli consists of tissue-cyst-forming coccidi ...200010952268
intestinal spore-forming protozoa among patients suffering from chronic renal failure.cryptosporidium parvum, isospora belli, cyclospora cayetanensis and microsporidia are four intestinal spore-forming protozoa that cause diarrhoea in immuno-competent individuals and immuno-suppressed patients. fresh stool samples were obtained from 120 patients suffering from crf and attending the dialysis unit of zagazig university hospital. also, stool samples were obtained from 40 immuno-competent individuals complaining of diarrhoea (control group). the stool samples were examined by direct ...200010786022
human infection with isospora belli; report of a case in florida. 195514387965
comparison of autofluorescence and iodine staining for detection of isospora belli in feces.to evaluate the sensitivity of autofluorescence for detection of isospora oocysts, wet preparations of 192 stool samples from patients with chronic diarrhea were examined by fluorescence microscopy and by light microscopy after iodine staining used for routine screening for ova and parasites. silicon-chambered glass coverslips were used for fluorescence microscopy. isospora oocysts were detected in 46 iodine-stained concentrated stool samples; 91 samples were positive by autofluorescence. accord ...200212408672
[contribution to the study of isospora belli infestations in children]. 196514322228
isosporiasis in children.the clinical, laboratory and therapeutic data of patients with diarrhea in human immunodeficiency virus (hiv) antibody positive, immunocompromised and immunocompetent individuals were studied especially to look for prevalence of intestinal coccidiosis. during a study period of one decade, isospora belli were identified in the stool samples of seven children with diarrhea. diarrhea persisted for a little longer period in hiv-seropositive children compared to sero-negatives but the clinical pictur ...200212428040
detection of isospora belli by polymerase chain reaction using primers based on small-subunit ribosomal rna sequences.the aim of the present study was to use small-subunit (ssu)-rrna sequences of isospora belli to design specific primer pairs and a hybridization probe for the detection of isospora belli in human samples by pcr and southern blot hybridization. pcr amplification with the primer pairs produced correct dna fragments with target dna from samples of isospora belli-infected patients and from cloned ssu-rrna of isospora belli. the nature of the pcr products was confirmed by southern blot hybridization. ...200011014628
isospora belli infection and chronic electrolyte disturbance in a child with fetal alcohol syndrome.we report an 11-year-old boy with fetal alcohol syndrome and immunodeficiency, whose longstanding malnutrition, diarrhoea with steatorrhoea, symptomatic electrolyte loss and eosinophilia were attributed to renal tubular disease and recurrent worm infestation. the symptoms resolved with diagnosis and treatment of infection by the protozoan isospora belli, although immunodeficiency persisted.200011130350
the role of dogs in transmission of gastrointestinal parasites in a remote tea-growing community in northeastern india.the prevalence and risk factors associated with canine gastrointestinal parasitic zoonoses and the role of dogs in the mechanical transmission of human ascaris infection was examined in three tea estates in assam, india. nearly all (99%) dogs harbored one or more zoonotic species of gastrointestinal parasites, with hookworm infection being most common (94%). parasitic stages presumed to be host-specific for humans such as ascaris spp. (31%), trichuris trichiura (25%), and isospora belli (2%) wer ...200212479559
[a case of human intestinal coccidiosis caused by isospora belli (wenyon, 1923)]. 196014437908
foodborne and waterborne parasites.more than 72 species of protozoan and helminth parasites can reach humans by food and water, and most of these infections are zoonoses. some parasites show a cosmopolitan distribution, others a more restricted distribution due to their complex life cycles, which need the presence of one or more intermediate hosts. of this large number of pathogens, only toxoplasma gondii can be transmitted to humans by two different ways, i.e., by cysts present in infected meat and by oocysts contaminating food ...200315058817
pictorial cme. isospora belli and cyclospora cayetanensis in a case of chronic diarrhoea in an immunocompromised host. 200011280226
[investigation of intestinal parasites in aids patients].in this study, enteric parasites were investigated in the stool samples of 38 aids patients (23 with chronic diarrhea and 15 without diarrhea) prospectively. at least three stool samples from each patient were investigated microscopically for ova or trophozoites. the samples were concentrated with formol-ether method and wet preparations stained with lugol were examined. in addition, the concentrated samples were stained with modified asid-fast (kinyoun's), rhodamine-auramine, modified trichrom ...200415293911
myxobolus sp., another opportunistic parasite in immunosuppressed patients?during a study of intestinal parasitic infections in human immunodeficiency virus-positive patients, a parasite belonging to the phylum myxozoa, recently described from human samples, was identified in one sample. when this parasite was stained by the modified ziehl-neelsen staining method, the features of the spores were identified: they were pyriform in shape, had thick walls, and had one suture and two polar capsules, with each one having four or five coils. the suture and two polar capsules ...200111326017
[discovery of isospora belli in duodenal juice]. 195914429128
isosporosis and unizoite tissue cysts in patients with acquired immunodeficiency syndrome.isospora belli, a coccidian parasite in humans, has been described as causing chronic diarrhea and acalculous cholecystitis in patients with the acquired immunodeficiency syndrome (aids). diagnosis can be made at the tissue level in the epithelium of the small bowel and by fecal examination. disseminated extraintestinal forms are uncommon. we studied 118 adult patients with aids and chronic diarrhea using stool analysis and endoscopy with duodenal biopsy specimen collection. these samples were p ...200111381368
nocardiasis in patients with hiv infection.the frequency of nocardia infection in hiv-infected patients has increased during the past few years from 0.3% in 1985 to 1.8% in 1989. although it is not of great concern as an aids-associated infection, the nonspecific clinical presentation in these patients might be confused with other lung infections such as tuberculosis (tb). the mortality rate can be as high as 60%. the authors diagnosed three homosexual men with nocardiasis among 1060 hiv-infected patients (0.2%) in a tertiary care center ...199811362038
intestinal parasites in hiv-2 associated aids cases with chronic diarrhoea in guinea-bissau.previous studies from african countries where hiv-1 infection is prevalent have shown that infections with cryptosporidium parvum, isospora belli and microsporidia are frequently associated with chronic diarrhoea in aids patients. the information about the occurrence of these parasites in hiv-2 associated aids cases with chronic diarrhoea is limited. we have performed a study of stool parasites in patients from guinea-bissau, the country with the highest prevalence of hiv-2 in the world. stool s ...200111495643
cyclospora cayetanensis, a food- and waterborne coccidian parasite.food- and waterborne coccidia including cryptosporidium parvum, cyclospora cayetanensis, sarcocystis hominis and sarcocystis suihominis, and isospora belli are cyst-forming apicomplexan protozoa that cause intracellular infections, predominantly in the epithelial cells of the intestine. they are transmitted by oocysts from person-to-person by the fecal-oral route or via contaminated water or food. the most common symptom of infection is diarrhea, however, asymptomatic infections occur. infection ...200415567580
isospora belli infection: observation of unicellular cysts in mesenteric lymphoid tissues of a brazilian patient with aids and animal inoculation.we describe the finding of unizoic cysts of isospora belli in lymphoid tissues of a brazilian patient with aids, and discuss the possibilities of their drug resistance, they being the cause of relapses, and of being an indication for the existence of intermediary or paratenic animal hosts.200314736218
infections by protozoa in immunocompromised hosts.protozoa are among the most important pathogens that can cause infection in immunocompromised patients. they infect particularly individuals with impaired cell immunity, such as those with hematologic neoplasias, those submitted to transplant of solid organs, those under high-dose corticosteroid therapy, and carriers of the human immunodeficiency virus. among the protozoa that most commonly cause disease in immunocompromised individuals are toxoplasma gondii, trypanosoma cruzi, cryptosporidium p ...200011142706
parasitic infections in hiv-infected patients who visited seoul national university hospital during the period 1995-2003.the prevalence of parasitic infections was investigated in human immunodeficiency virus (hiv)-infected patients (n = 105) who visited seoul national university hospital, seoul, korea, during the period from 1995 to 2003. fecal samples were collected from 67 patients for intestinal parasite examinations, and sputum or bronchoalveolar lavage samples from 60 patients for examination of pneumocystis carinii. both samples were obtained from 22 patients. thirty-three (31.4%) of the 105 were found to h ...200515793352
coccidiosis among malnourished children in mansoura, dakahlia governorate, egypt.stool samples of 36 malnourished and 36 healthy control children were examined for oocysts of cryptosporidium parvum, cyclospora cayetanensis, isospora belli and microsporidium. c. parvum infection (single and mixed) was detected in 13.9% and 5.6% in malnourished and control children respectively. c. cayetanensis oocysts were detected in 5.6% malnourished children and in 2.8% of control group. i. belli oocysts were detected only in malnourished children 2.8%. on the other hand, no microsporidium ...200111775113
[gastrointestinal symptoms in acquired immunodeficiency syndrome (aids): a review of one hundred cases at "arzobispo loayza" hospital]in 1996, one hundred health histories of hiv serum positive patients were reviewed using the elisa and western blot techniques at the arzobispo loayza national hospital. such cases were defined according to case definition 1987 cdc/who. 70% of hiv serum positive patients showed gastrointestinal symptoms according to the first medical examination. 57% of the patients were younger than 30 years old, most of them were men (men/ women ratio: 2.7/1). 59% of the patients were heterosexual, 26% bisexua ...199712177715
survey for intestinal parasites in belize, central america.a stool survey was carried out in 5 villages in the toledo district of the central american country of belize. eighty-two percent of a total population of 672 participated. the stools were examined by the formalin-ethyl-acetate concentration technique. sixty-six percent of the population was found to have one or more intestinal parasites. the most common infection was hookworm (55%) followed by ascaris lumbricoides (30%), entamoeba coli (21%), trichuris trichiura (19%), giardia lamblia (12%), io ...200415689057
steatorrhea in a man infected with coccidiosis (isospora belli). 196414234685
[infective etiology of diarrhea in adults with hiv infection in dakar: a case-control study on 594 patients].a survey was conducted in dakar, senegal from may 1997 to may 1999, to identify major types and prevalences of bacteria, parasites, fungi and rotaviruses associated with diarrhea in relation to human immunodeficiency virus serostatus with the aim to provide guidance to physicians for case management. etiologic agents were identified in a case-control study: cases were hiv-infected patients with diarrhea (hiv+ d+) and hiv seronegative patients with diarrhea (hiv- d+); controls were hiv-infected p ...200115773157
aids-associated diarrhea and wasting in northeast brazil is associated with subtherapeutic plasma levels of antiretroviral medications and with both bovine and human subtypes of cryptosporidium parvum.advanced hiv infection is frequently complicated by diarrhea, disruption of bowel structure and function, and malnutrition. resulting malabsorption of or pharmacokinetic changes in antiretroviral agents might lead to subtherapeutic drug dosing and treatment failure in individual patients, and could require dose adjustment and/or dietary supplements during periods of diarrheal illness. we determined the plasma levels of antiretroviral medications in patients that had already been started on medic ...200312807688
[intestinal parasitosis: our series from 1992 to 1995].from 1992 to 1995 in our department 2236 researches of intestinal parasites (2194 on stool specimens, 34 on scotch tests, 8 on enterotests) were carried out on 1200 patients (703 hiv-ab negative and 497 hiv-ab positive). on the whole 387 samples (17.34%) of 203 subjects (16.92%) were found parasitized; 92 (13.08%) patients of them were hiv-ab negative and 111 (22.33%) were hiv-ab positive. we found more frequently blastocystis hominis and giardia intestinalis among hiv-ab negative subjects and c ...199612858028
[1st case of human coccidiosis caused by isospora belli reported in belgium]. 196414277145
[gastrointestinal manifestations of aids in adults in mali].our main objective consists in evaluating the frequency of digestive signs and digestive opportunistic infections in aids patients with diarrhea. the prospective study occurred from january 1997 to july 1998 in bamako hospitals. the patients underwent a clinical examination, blood and stools tests, and sometimes upper digestive endoscopy. among 434 cases of aids, 426 patients (98%) had at least one digestive sign. the main digestive signs were diarrhea (80.1%), abdominal pains (62.2%), vomiting ...200212596372
sequence analysis of partial regions of the 5.8s rrna internal transcribed region 2 and 28s rrna of isospora belli. 200312866923
[encapsulated cord method(enterotest) compared with fecaltest on the diagnosis of criptosporium sp e isospora belli in hiv(+)patients with gastrointestinal symptoms]fecal samples and small intestinal content obtained by enterotest were submitted between december 1994 and january 1996, by hiv (+) patients with gastrointestinal symptoms.unconcentrated and concentrated fecal samples (by sheater, ritchie and spontaneous sedimentation) and intestinal content obtained by enterotest, all stained by modificated ziehl-nielsen method, were compared (multiatributive comparison) based on the following parameters: yield, each test cost, morphology and the number of para ...199812215737
important zoonotic intestinal protozoan parasites in asia.intestinal protozoa are increasingly being studied because of their association with acute and chronic diarrhoea in immunocompromised as well as immunocompetent patients. various community outbreaks due to contamination of water or food with these protozoa have further highlighted their importance in public health. among these important pathogens are giardia duodenalis, entamoeba histolytica, cryptosporidium parvum, cyclospora cayetanensis, isospora belli, and microsporidia. except for the cyst- ...200416493397
[extraintestinal finding of isospora belli unizoic cysts in a patient with aids: case report].this report describes the presence of isospora belli unizoic cysts in mesenteric lymph nodes and of gametocytes in the gallbladder epitelium of a 26 year-old brazilian male patient with acquired immune deficiency syndrome. this patient had received treatment for several times with sulfamethoxazole-trimethoprim. it is discussed the significance of i. belli tissue cysts as possible foci of resistance of the parasite and their association with the infection relapse even post-treatment with anticocc ...200312908043
mechanical transmission of pathogenic organisms: the role of cockroaches.cockroaches (diploptera punctata) are basically tropical insects and will do their best to find a home that is both warm and moist. their involvement in the transmission of tropical diseases is poorly investigated in africa.200516457381
cryptosporidium parvum and isospora belli infections among patients with and without diarrhoea.to assess the importance of cryptosporidium parvum and isospora belli infections as a cause of diarrhoea among patients admitted to the medical wards in queen elizabeth central hospital (qech) in blantyre, malawi.200314601779
cryptosporidium parvum and other instestinal parasites among diarrhoeal patients referred to ehnri in ethiopia.the occurrence of intestinal parasites in patients referred to the parasitology laboratory of the ethiopian health and nutrition research institute (ehnri), was recorded for four consecutive years, 1998-2001. based on the stool appearance i.e. watery, loose, muciod and bloody, 442 diarrhoeal samples were particularly selected to allow diagnosis of intestinal parasites with emphasis on coccidian intestinal parasites. wet saline mounts and formol ether concentration method with logul's iodine were ...200416895037
waterborne transmission of protozoan parasites: a worldwide review of outbreaks and lessons learnt.at least 325 water-associated outbreaks of parasitic protozoan disease have been reported. north american and european outbreaks accounted for 93% of all reports and nearly two-thirds of outbreaks occurred in north america. over 30% of all outbreaks were documented from europe, with the uk accounting for 24% of outbreaks, worldwide. giardia duodenalis and cryptosporidium parvum account for the majority of outbreaks (132; 40.6% and 165; 50.8%, respectively), entamoeba histolytica and cyclospora c ...200717402277
cellular immunity and diarrhoeal disease amongst patients infected with the human immunodeficiency viruses 1 and 2 in zaria, nigeria.this study was carried out to determine the aetiological agents associated with diarrhoea in nigerians infected with the human immunodeficiency viruses, and to correlate the cellular immunity status with the causes of diarrhoea. the mean duration of diarrhoea was 20.7 weeks. giardia lamblia was the commonest pathogen and was isolated from the stools of 10 patients (25%). isospora belli was isolated in 3 of the patients (7.5%) but in none of the controls. other pathogens isolated include salmonel ...200312956003
needle necropsy in aids.the value of autopsy in understanding the natural course of any disease is beyond any argument. the reluctance of pathologists to perform autopsy in hiv infected cadavers is justified due to the risks involved to the prosector and the morgue attendants. a relative low risk needle necropsy protocol is proposed using fine needle aspiration cytology, tru-cut biopsies and microbiological examination. diagnosis could be offered in all the forty-four needle necropsies performed. disseminated tuberculo ...200315025288
[evaluation of parasitological laboratory results of a group of people older than 15 years of age in kutahya].this research has been based on microscopic examination of samples of stool specimens taken from 675 persons older than 15 years of age. the examinations were conducted in the parasitological laboratory of the dumlupinar university hospital. laboratory examinations of stools were done with native and formalin-ethyl acetate sedimentation methods. different intestinal parasites were found in 52 (7.7%) of the stool specimens. at least one parasite species was found in 3.11% of women and 4.59% of me ...200717471410
case report: nitazoxanide treatment failure in chronic isosporiasis.we report a 60-year-old immunocompetent patient with chronic biliary isosporiasis who failed to respond to orally administered cotrimoxazole prophylaxis and orally administered treatment with nitazoxanide, a 5-nitrothiazole benzamide compound. severe malabsorption was regarded as responsible for the subtherapeutic levels of nitazoxanide in plasma and bile, resulting in treatment failure. intravenously administered cotrimoxazole stopped the shedding of isospora belli oocysts in bile within 5 days ...200111508398
a case of human infestation with isospora belli in egypt. 196313982276
prevalence of intestinal parasites including microsporidia in human immunodeficiency virus-infected adults in cameroon: a cross-sectional study.to assess the prevalence of intestinal parasites in a cohort of human immunodeficiency virus (hiv)-infected adults in cameroon, a cross-sectional study was conducted. detection of parasites was performed in 181 stool samples from 154 hiv-infected patients with a mean cd4 cell count of 238 cells/mm(3). only 35 patients (22%) were receiving antiretroviral therapy at the time of stool sampling, and 46 (29%) had diarrhea. opportunistic protozoa were found in 15 patients (9.7%), 8 of whom (53%) had d ...200616407362
point prevalence of cryptosporidium, cyclospora, and isospora infections in patients being evaluated for diarrhea.from march to september 2001, 315 specimens from "nonrepeat" patients that were submitted for ova and parasite examination were stained using the kinyoun modified acid-fast stain to detect the intestinal coccidians. four patients (1.3%) were infected with coccidians, 2 with cryptosporidium parvum and 2 with cyclospora cayetanensis. no infections with isospora belli were detected. in comparison, 15 patients (4.8%) had infections with one or more intestinal parasites detected by routine trichrome ...200415272527
exflagellation of microgametocytes in plasmodium vivax malaria: a diagnostic conundrum.to present a clinical diagnostic conundrum of unidentified structures in a blood smear from a patient with plasmodium vivax malaria.200415316267
intestinal parasitic infections in hiv/aids patients: experience at a teaching hospital in central brazil.in order to verify the occurrence of intestinal parasitic infections in human immunodeficiency virus (hiv)/acquired immunodeficiency syndrome (aids) patients, 100 hiv/aids patients (group 1) and 85 clinically healthy individuals (group 2) were submitted to coproparasitological examination. intestinal parasites were detected in 27% of patients from group 1 and in 17.6% from group 2. in group 1 the most frequent parasites were strongyloides stercoralis (12%), with 2 cases of hyperinfection; isospo ...200515849055
case report: isospora belli infection in a renal transplant recipent.isospora belli, an opportunistic protozoon, is one of the most commonly recognized causes of diarrhea in patients with acquired immunodeficiency syndrome (aids). infection is acquired through fecal contaminated food or water, and generally diagnosed by examination of stool and/or duodenum biopsy specimens with acid-fast staining. here, we present an uncommon case, chronic diarrhea caused by i. belli infection in a patient who is a renal transplant recipient.200717594646
[intestinal parasitosis in patients with hiv-aids].diarrhea, a common complication and one of the most important causes of malabsorption and malnutrition in aids patients. our objective was to determine the parasitic causes of diarrhea in patients with hiv-aids at the cayetano heredia national hospital (lima, peru).200616622484
[evaluation of parasitology laboratory results of a group of people older than 15 years of age in kutahya].this research has been based on microscopic examination of stool specimens taken from 675 persons older than 15 years of age. the examinations were conducted in parasitology laboratory of the dumlupinar university hospital. laboratory examinations of stools were done with native and formalin-ethyl acetate sedimentation methods. different intestinal parasites were found in 52 (7.7%) of the stool specimens. at least one parasite species was found in 3.11% of women and 4.59% of men. the prevalence ...200717594654
high proportion of isosporiasis among hiv-infected patients with diarrhea in southern india.we investigated 245 diarrheal stool specimens from hiv-positive subjects between january 2003 and december 2006 to determine the etiological role of coproparasites. parasitic etiology was observed in 91 (37.1%) cases. isospora belli (26.1%) was the most common parasite followed by entameba histolytica/dispar (3.3%), cryptosporidium spp. (2.9%), giardia intestinalis (1.6%), and strongyloides stercoralis (1.2%). interesting trends of significant increase in the number of cases of i. belli and decl ...200717984334
intestinal parasitic infection among children and neonatus admitted to ibn-sina hospital, sirt, libya.a total of 350 stool samples from 196 males and 154 female children and neonatus admitted in ibn-sina hospital, sirt, were examined from june 2001 to may 2002, to determine the prevalence of intestinal parasites. intestinal parasitic infections were identified in 196 (56%) of children and neonates. no intestinal helminthic parasites were detected but 13 intestinal protozoan parasites were detected. the most prevalent protozoan was entamoeba histolytica /e. dispar (36.57%); blastocystis hominis ( ...200717985574
[intestinal parasitosis during aids diarrhoea].the main goal of this work was to study the parasitic infections during aids diarrhoea. a longitudinal study was carried out from october 2000 to november 2001 in the hepato-gastro-enterology department of gabriel toure hospital and in the department of epidemiology and parasitic infections of faculty of medicine, pharmacy and odonto-stomatology of bamako (mali), concerning hiv positive patients suffering from diarrhoea. detecting microbes in stools has been done through direct microscopic exami ...200515915971
[cryptosporidiosis and isosporiasis in children suffering from diarrhoea in abidjan].a parasitological survey on intestinal coccidia, cryptosporidium sp and isospora belli, has been carried out in paediatric service of an teaching hospital in the district of abidjan (côte d'ivoire). 130 children under 5 years old suffering from diarrhoea have been enrolled. direct stools examination method, simplified ritchie technique and ziehl neelsen modified coloration were used for analysis. moreover, the aids serological status of the patients has been determined. the results show that cry ...200417304752
evaluation of an enzyme-linked immunoassay for the detection of cryptosporidium antigen in fecal specimens of hiv/aids patients.cryptosporidium parvum, a protozoan parasite, causes severe diarrhea in immunodeficient hosts like hiv/aids patients, leading to significant morbidity and mortality. diagnosis of the cryptosporidium oocyst in the stool of these patients by conventional microscopy is labor intensive and time consuming. therefore, we planned to evaluate the usefulness of a stool elisa test in detecting cryptosporidial antigen. about 89 stool specimens obtained from hiv-seropositive patients with diarrhea were subj ...200818417886
[1st case of human coccidiosis due to isospora belli reported in belgium]. 196213930677
[high prevalence of cyclospora cayetanensis among indigenous people in bolivar state, venezuela].the intestinal coccidioses caused by cryptosporidium parvum, isospora belli, and cyclospora cayetanensis are parasitoses of major medical importance, but many epidemiological aspects of these infections are still unknown in bolívar state, venezuela. to determine the prevalence of intestinal parasites and especially of intestinal coccidiosis, an indigenous population in san antonio de morichal was evaluated from july 2003 to april 2004. stool samples obtained by spontaneous evacuation were preser ...200616410862
cystoisospora belli: in vitro multiplication in mammalian cells.intracellular development of cystoisospora belli was demonstrated in 4 different mammalian cell lines. human ileocecal adenocarcinoma (hct-8), epithelial carcinoma of lung (a549), madin-darby bovine kidney (mdbk), and african green monkey kidney (vero) were exposed in vitro to c. belli sporozoites, which had been isolated from the feces of hiv-aids patients. parasites invaded all the cellular types between 4 and 12h after exposure and multiplication was demonstrated after 24 h. grater number of ...200616674944
cryptosporidiosis in hiv-infected venezuelan adults is strongly associated with acute or chronic diarrhea.a cross-sectional study was undertaken to determine the prevalence of cryptosporidiosis and its clinical and laboratory pattern in venezuelan hiv-infected patients (n = 397). at enrollment, they underwent thorough clinical history and physical examination and provided stool specimens for the identification of cryptosporidium sp. and other parasites. cryptosporidium sp. was identified in 59 subjects (15%). this infection was strongly associated with acute and chronic diarrhea, weight loss, cd4(+) ...200516014832
enteric parasitic infections in hiv/aids patients before and after the highly active antiretroviral therapy.enteroparasites are related to gastrointestinal alterations among patients with hiv/aids, some causing severe manifestations in the period before the institution of the highly active antiretroviral therapy (haart). the prevalence of enteroparasitoses in patients with hiv/aids seen at two hospitals in ceará , brazil, was compared in the pre-haart (group 1; n = 482) and haart (group 2; n = 100) eras. fecal parasitologic examinations (fpe) were performed using the direct, lutz, baermann-moraes and ...200818641847
hiv-associated isospora belli infection: incidence and risk factors in the french hospital database on hiv.to determine the incidence of isospora belli infection in hiv-infected patients in france, and to study risk factors.200717352769
isospora belli infection in a patient with non-hodgkin's lymphoma.isospora belli infection is frequent in patients with acquired immunodeficiency syndrome in tropical areas. it has also been reported in other immunodepressive diseases, such as lymphoblastic leukemia, adult t-cell leukemia, and hodgkin's disease. to date, no case of non-hodgkin's lymphoma-related isosporiasis has been reported in a non-hiv-infected patient. we describe a case of non-hodgkin's lymphoma with chronic diarrhea due to i. belli. in europe, i. belli can cause severe chronic diarrhea i ...200314616755
[parasitoses and immunodepression].the immunodepression, related or not to aids, induces the emergence of opportunistic parasitosis and mycosis. our objective is to analyze these pathogenic agents, their clinical expression and gravity in immunocompromised individuals. our retrospective 9 years study reported parasitic and fungic infections complicating immunodepression. among 31 hiv infected patients, we diagnosed the following parasitosis: cryptosporidium (7 cases), isospora belli (2 cases) and enterocytozoon bieneusi (1 case). ...200014658228
[short communication: investigation of intestinal coccidia in patients with diarrhea].the intestinal protozoa have been increasingly identified in immunocompromised patients. in this study, stool samples of 554 patients [288 male, 266 female; 11 (2%) of them were immunocompromised] with diarrhea were examined between june 2004 and june 2005 in the parasitology laboratory of dokuz eylul university hospital, izmir, turkey, in order to investigate the prevalence of intestinal coccidia. after formalin-ethyl acetate sedimentation procedure, the samples were examined by both native lug ...200717427562
enteric opportunistic parasites among hiv infected individuals: associated risk factors and immune status.data on various etiologic agents causing diarrhea in human immunodeficiency virus type-1 (hiv-1) infected individuals are sparse in delhi, india. the present study was undertaken to identify various causative agents, the role of associated risk factors and immune status. a case-control study was conducted among 75 hiv-1 infected individuals, 50 with and 25 without diarrheal infection. fecal samples were screened for coccidian parasites, enteric protozoa, and helminthes by using various staining ...200717515636
microscopic diagnosis of sodium acetate-acetic acid-formalin-fixed stool samples for helminths and intestinal protozoa: a comparison among european reference laboratories.the present study aimed to compare the diagnostic performance of different european reference laboratories in diagnosing helminths and intestinal protozoa, using an ether-concentration method applied to sodium acetate-acetic acid-formalin (saf)-preserved faecal samples. in total, 102 stool specimens were analysed during a cross-sectional parasitological survey in urban farming communities in côte d'ivoire. five saf-preserved faecal samples were prepared from each specimen and forwarded to the pa ...201019456836
isosporiasis in venezuelan adults infected with human immunodeficiency virus: clinical characterization.a cross-sectional study was undertaken to determine the prevalence of isosporiasis and its clinical and laboratory pattern in venezuelan patients infected with human immunodeficiency virus (hiv) (n = 397). at enrollment, they underwent a thorough clinical history and physical examination, and provided stool specimens for the identification of isospora belli and other parasites. isospora belli was identified in 56 subjects (14%) and diarrhea, either acute or chronic, was present in 98% of these c ...200313677379
endometrial coccidiosis.this report describes a case of granulomatous endometritis caused by coccidiosis in an immunologically uncompromised 63 year old patient. the glandular epithelium of the endometrium contained numerous intracytoplasmic cysts, corresponding to periodic acid schiff positive and methenamine silver negative sporoblasts. the endometrial glands revealed reactive phenomena, such as eosinophilic and squamous glandular metaplasia and intraluminal desquamation. non-necrotising epithelioid granulomata, lack ...200415452171
[isospora belli infection in a patient with a renal transplant].isospora belli infection is frequent in immunosuppressed patients and can cause wasting diarrhea. we present the first isosporiosis case in a renal transplant recipient from turkey. the 25-year old male patient who had had a renal transplantation due to renal failure and had received immunosuppressive therapy presented at the hospital complaining of weakness, nausea, vomiting and diarrhea that had lasted for 15 days. isospora belli oocysts were detected in stool samples by direct microscopy, mod ...200617106849
[parasitic infection in immunocompromised patients].several parasites are responsible for life threatening infections in immunocompromised patients. they occur in patients with a profound immunodeficiency affecting the t-cell mediated immunity. in aids patients, opportunistic infections are highly prevalent in those with cd4 lymphocyte counts < 200/mm3. most of these parasites are intracellular protozoa. severe parasitic infections in immunocompromised hosts either results from the reactivation of a previously acquired infection, such as toxoplas ...200717432521
opportunistic parasitic infections in hiv/aids patients presenting with diarrhoea by the level of immunesuppression.enteric parasites are major cause of diarrhoea in hiv infected individuals. the present study was undertaken to detect enteric parasites in hiv infected patients with diarrhoea at different levels of immunity.200919700803
isospora belli infection after isolated intestinal transplant.isospora belli is a protozoan that only affects humans, after ingestion of isospora's oocysts. immunocompetent patients usually do not develop the infection. immunocompromised hosts may have profuse diarrhea with other gastrointestinal symptoms. treatment is based on trimethoprim-sulfamethoxazole. in 2006 we performed an isolated intestinal transplantation in a patient with ultra-short bowel syndrome. neither rejection nor clinical problems occurred after transplant, but signs of intestinal infl ...201019708892
development of isospora belli in hct-8, hep-2, human fibroblast, bek and vero culture cells.the development of isospora belli, a human coccidian parasite, was studied in different cell lines. merozoites were observed in all kinds of cells, whereas sporogony was demonstrated only in hct-8. this implied that not only the human cell line can be infected, but also some animal cell lines. unizoites could be found in vero cells. the merozoites were transferred to a new culture cell for three passages and maintained for two weeks, but no oocyst production was observed in any culture cells dur ...200415916071
[not available]. 195015405241
parasites in patients with malabsorption syndrome: a clinical study in children and adults.intestinal parasites not only cause diarrheal diseases but also significant malabsorption. literature on the role of parasites, such as intestinal coccidia and microsporidia in malabsorption syndrome is limited.200817763958
[intestinal parasitosis in french west indies: endemic evolution from 1991 to 2003 in the university hospital of pointe-a-pitre, guadeloupe].guadeloupe is one of the french west indies, where, until 1960, intestinal parasitic infections were endemic. in the microbiological laboratory of the university hospital of the island, we carried out a retrospective study of the 17,660 stool examinations received from 1991 to 2003. all the stool specimens were examined using at least a wet mount preparation and a concentration method (bailenger). specific techniques such as ziehl-neelsen modified acid fast stain, chromotrope staining procedure ...200617111973
intestinal parasitic infection of immunocompromised children with diarrhoea: clinical profile and therapeutic response.parasitic gastrointestinal infections have been variably reported among immunocompromised adults while data on children have been limited. this prospective cross-sectional study aimed to assess the clinical profile of intestinal parasitic infections among immunocompromised children with diarrhoea and their treatment response.201020539063
[the etiological, clinical and prognosis of diarrhea occurs during aids in to hospital in bamako].its was a descriptive survey achieved in the service of the infectious and tropical illnesses of the teaching hospital of the "point g" from 31 december 2002 to 30 june 2004 was about the analysis of 76 files. its goal was to determine the clinical, etiological and prognostic aspects, of the diarrhea during the aids. the oral-pharyngeal candidiasis (50%), the deshydratation (60,5%), the fever (67,5%) and the thinning (77,6%) were associated to the diarrhoea frequently. cholera syndrome was (52.6 ...200719617111
intestinal parasites, including cryptosporidium species, in iraqi patients with sickle-cell anaemia.stool samples were obtained from individuals admitted to three hospitals in basra during november 1997-may 1998. of 40 patients with sickle-cell anaemia, 25 (62.5%) had parasitic infections. in the apparently healthy comparison group, 26 of 175 individuals (14.8%) had intestinal parasitic infections, a statistically significant difference. the most common intestinal parasites isolated in the sickle-cell patients were blastocystis hominis (36%) and giardia lamblia (28%). the isolation rate of cry ...200215339123
[a retrospective evaluation of the prevalence of intestinal parasites in istanbul, turkey].the aim of this study is to estimate the prevalence of intestinal parasites, in comparison to previous studies in istanbul and different geographic regions of turkey. between january 1999-december 2009, a total of 27664 stool samples from patients with suspicious intestinal parasitic infections who were admitted to istanbul university cerrahpasa medical school hospital were examined for intestinal parasites by using native lugol and formalin-ethyl acetate concentration technique, in adition to t ...201020954117
a rare diarrheic parasite in a liver transplant patient: isospora belli.we describe the first case of isosporiasis in a liver transplant patient. watery diarrhea due to isospora belli was observed in a woman who had undergone liver transplantation 8 months prior. she was successfully treated with trimethoprim-sulfamethoxazole. this parasite should be taken into consideration as an opportunistic infection in transplant patients who need increased hygienic awareness.200717580223
protozoal pollution of surface water sources in dakahlia governorate, egypt.water samples were collected different water sources and different districts of dakahlia g., from september 2005 to august 2006, were investigated for pathogenic protozoa. water specimens were examined by concentration technique followed by modified ziehl-neelsen (mzn) and truant auramine-rhodamine (ar) stains for giardia sp. cysts, cryptosporidium sp. oocysts, and other protozoa species. in the 1st survey, 94/480 (19.6%) water samples had protozoa. prevalence rates were in summer 38/120 (31.6%) ...200717580568
chronic diarrhoea in hiv patients: prevalence of coccidian parasites.the purpose of this study was to determine the prevalence of intestinal parasites in hiv patients with or without diarrhoea and to see an association between diarrhoea and the coccidian parasites in our setting. stool samples from 113 hiv patients, 34 chronic diarrhoea and 79 without any history of diarrhoea were collected and examined for enteric parasites by microscopy. one hundred and thirteen control samples from hiv negative patients complaining of prolonged diarrhoea were also collected an ...200818445958
[not available]. 194915399473
screening of auramine-stained smears of all fecal samples is a rapid and inexpensive way to increase the detection of coccidial infections.coccidia are important causes of diarrhea that is often indistinguishable from other forms of community-acquired diarrhea. however, the detection of oocysts is often only performed when explicitly requested, as part of the ova and parasite (o&p) examination. reappraisal and understanding of the accurate staining characteristics of auramine o (auo), which stains nucleic acids, may permit the inexpensive and reliable identification of coccidian oocysts at routine workup of all fecal samples.200817600749
prevalence of intestinal parasitic infections among hiv patients in benin city, nigeria.this study was carried out to determine the presence of intestinal parasites and their correlation with cd4(+) t-cell counts and demographics among human immunodeficiency virus (hiv)-positive patients in benin city, nigeria. stool specimens from 2,000 hiv-positive patients and 500 controls (hiv-negative individuals) were examined for ova, cysts, or parasites, using standard procedures. in addition, patient's blood samples were analyzed for cd4 counts by flow cytometry. an overall prevalence rate ...201021483561
use of nitazoxanide as a new therapeutic option for persistent diarrhea: a pediatric perspective.despite advances in the management of diarrheal disorders, diarrhea is the second most frequent illness in the world. persistent diarrhea, common in community pediatrics, is often caused by organisms such as giardia lamblia, cryptosporidium parvum and, less frequently, cyclospora, isospora belli, and clostridium difficile. identifying the causative organism is often challenging, and diagnostic tests may be inaccurate and expensive and, thus, of limited benefit. consequently, carefully chosen emp ...200516004666
how common is intestinal parasitism in hiv-infected patients in malaysia?human immunodeficiency virus (hiv)-infected individuals have greater susceptibility to infections by a myriad of microorganisms which can cause significant morbidity and mortality compared to immunocompetent individuals. of these microbial infections, intestinal parasitic infections (ipis) however are receiving less attention than bacterial and viral infections, hence, the lack of information of parasitic infections in hiv individuals. prevalence of ipis among 346 hiv-infected individuals in mal ...201122041762
intestinal parasitic infection in hiv infected patients with diarrhoea in chennai.to determine the prevalence of intestinal parasites in hiv patients with and without diarrhoea in chennai.200217657039
the plastid-like organelle of apicomplexan parasites as drug target.apicomplexan parasites infectious to humans include plasmodium spp., babesia spp., toxoplasma gondii, cryptosporidium spp., isospora belli and cyclospora cayetanensis. with exception of cryptosporidium spp., these parasites possess a non-photosynthetic plastid-like organelle called apicoplast. the apicoplast possesses a small circular genome and harbours prokaryotic-type biochemical pathways. as the most important metabolic functions, the mevalonate independent 1-deoxy-d-xylulose 5-phosphate pat ...200818473835
presence of enterocytozoon bieneusi associated with intestinal coccidia in patients with chronic diarrhea visiting an hiv center in haiti.this study investigated the presence of enterocytozoon bieneusi as a possible cause of chronic diarrhea in haitian patients attending the gheskio aids clinic in port-au-prince, haiti. coccidian oocysts were found by polymerase chain reaction (pcr) in the stools of 58/74 patients with chronic diarrhea and included the following agents: 45 (60%) cryptosporidium spp., 27 (34%) cyclospora cayetanensis, and 11 (15%) isospora belli. four patients (5.5%) were co-infected with e. bieneusi and one (1.4%) ...200818840748
experimental infection of murine and human macrophages with cystoisospora belli.extraintestinal cystoisosporosis by cystoisospora belli has already been reported in hiv/aids patients, generally involving preferential invasion of mesenteric and trachaeobronchial lymph nodes, liver and spleen by unizoic cysts of this parasite, which may infect macrophages. to test this hypothesis, murine and human macrophages were exposed to sporozoites of c. belli and cultures were observed daily after contact with these cells. the parasites penetrated and multiplied by endodyogeny in both c ...200919427297
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