Publications

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haematology of spanish ibex (capra pyrenaica hispanica) restrained by physical or chemical means.reference values for some haematological and plasma biochemical constituents were established in spanish ibex (capra pyrenaica hispanica) restrained either physically or chemically with tiletamine-zolazepam. the following variables were studied: haematocrit, haemoglobin concentration, total erythrocyte and leucocyte counts, haematological indices, erythrocyte dimensions, differential count of leucocytes, glucose, urea, uric acid, cholesterol, triglycerides, creatinine, aspartate aminotransferase ...19938337802
effect of captivity on the blood composition of spanish ibex (capra pyrenaica hispanica).blood analyses of seven free-ranging spanish ibex (capra pyrenaica hispanica) captured from the wild and then held in captivity were used to determine the physiological changes in some haematological parameters and serum chemistry values during captivity. the captive animals had a higher haematocrit and haemoglobin concentration and larger numbers of erythrocytes than the same animals when they were captured. in addition, the absolute numbers of leucocytes and lymphocytes decreased progressively ...19958748172
sarcoptic mange in spanish ibex from spain.the spanish ibex (capra pyrenaica hispanica) population of the "sierras de cazorla, segura y las villas" nature park (spain) was isolated as the result of a severe epidemic of sarcoptic mange. in this context, the dynamic characteristics of the disease were analyzed in a wild group consisting of 35 animals from the beginning of the epizootic (when the mating period started) to the extinction of the population due to mange. monthly tracking permitted the sequential characterization of the patholo ...199910574523
immunoglobulin g class identification from wild ungulates by cross-reactivity with antisera to domestic animals.seven species of spanish ungulates were tested for the presence of homologous immunoglobulin g (igg) with a gel-diffusion test using bovine, ovine, caprine and porcine igg antisera. homologous ovine and caprine igg were detected in sera from chamois (rupicapra pyrenaica), spanish ibex (capra pyrenaica hispanica), mouflon (ovis orientalis musimon), red deer (cervus elaphus), fallow deer (dama dama) and roe deer (capreolus capreolus). homologous porcine igg was detected in wild boar (sus scrofa) s ...200011014063
experimental ivermectin treatment of sarcoptic mange and establishment of a mange-free population of spanish ibex.ivermectin was used to treat sarcoptic mange in spanish ibex (capra pyrenaica hispanica). its therapeutic effectiveness was analyzed when it was administered through subcutaneous injection, to sick animals in the consolidation stage of mange (third phase) and, with double injections to chronically affected animals (fourth phase) at a dosage of 0.2 or 0.4 mg/kg body weight (bw). three wk after treatment, the animals in the third phase of mange treated with a high dose (0.4 mg/kg bw) of ivermectin ...200111763741
seasonal ovulatory activity and plasma prolactin concentrations in the spanish ibex (capra pyrenaica hispanica) maintained in captivity.seasonal changes in ovulatory activity, assessed by the measurement of plasma progesterone and plasma prolactin concentrations were monitored in 10 spanish ibex females captured in the national wildlife reserve of sierra nevada (south spain, 37 degrees n). five of the 10 female ibex showed ovulatory activity with a mean (+/- s.e.m.) duration of the oestrous cycle of 19.4 +/- 1 days (range: 17-23 days). on average, the five cyclic females weighed 28 +/- 0.9 kg. progesterone cycles occurred only i ...200314620629
relationship between bronchopulmonary nematode larvae and relative abundances of spanish ibex (capra pyrenaica hispanica) from castilla-la mancha, spain.the excretion of bronchopulmonary nematode infective larvae was evaluated in 160 faecal samples of spanish ibex (capra pyrenaica hispanica) collected from 13 populations in castilla-la mancha, south-central spain in september 2003. intensities and prevalences were compared with pasture availability, abundances of wild and domestic ungulates at both levels, i.e. for populations and for faeces in a two-step procedure. protostrongylid larvae showed similar infection rates (mean intensity: 1.56+/-0. ...200515946393
birth of live spanish ibex (capra pyrenaica hispanica) derived from artificial insemination with epididymal spermatozoa retrieved after death.as a consequence of increasing limitations to maintaining genetic variability in endangered wildlife species, methods of assisted reproduction widely used in domestic animals are being applied to nondomestic species. however, practical efforts have met limited success to date. the spanish ibex (capra pyrenaica hispanica) is a wild caprine originating exclusively in the mountains of spain. this study was designed to evaluate the fertilizing capability of cryopreserved spanish ibex epididymal sper ...200616376980
potential pathogens carried by spanish ibex (capra pyrenaica hispanica) in southern spain.the spanish ibex (capra pyrenaica hispanica) population of southern spain was surveyed for potential pathogens associated with the conjunctiva, external ear canal, as well as reproductive and upper respiratory tracts. we sampled 321 ibex (131 adult males, 100 adult females, and 90 yearlings); these included 271 apparently healthy animals and 50 that were naturally infected with sarcoptes scabiei. a total of 688 bacterial isolates were identified (377 gram-negatives, 225 gram-positives, and 86 my ...200616870855
horn quality and postmortem sperm parameters in spanish ibex (capra pyrenaica hispanica).the horns are secondary sexual characteristics used by males of many ungulate species for intra-sexual fights during the rut. thus, the dominant males with most developed horns are naturally selected for reproduction. several studies have suggested that the quality of the horn, in many wild ruminants, may be correlated with semen quality. the aim of the present study was to determine whether inter-individual differences in levels of horn asymmetry and horn size are related to differences in sper ...200716846702
mycoplasmas isolated from spanish ibex (capra pyrenaica hispanica): frequency and risk factors. 200717675635
horn growth related to testosterone secretion in two wild mediterranean ruminant species: the spanish ibex (capra pyrenaica hispanica) and european mouflon (ovis orientalis musimon).seasonal variations in the horn development and testicular activity of the spanish ibex (capra pyrenaica hispanica) (n=6) and european mouflon (ovis orientalis musimon) (n=5) were monitored to determine the role of increasing testosterone concentration on the arrest of horn growth during the rutting season. marked seasonal variations in the rate of horn growth (p<0.01) and testicular activity (p<0.001) were seen in both species, although the magnitude and timing of these changes were different ( ...200717123755
kit-positive gastrointestinal stromal tumours in two spanish ibex (capra pyrenaica hispanica).gastrointestinal mesenchymal tumours from two spanish ibex (capra pyrenaica hispanica) were examined grossly, histologically and immunohistochemically. one neoplasm was a 1.5 kg tan multinodular cavitated mass in the forestomach. the other tumour was a firm mural mass 1.2 cm in diameter in the colon. microscopically, both tumours were formed mainly by spindle shaped cells arranged in closely packed interlacing fascicles. neoplastic cells in both tumours labelled positively for kit (cd117), vimen ...200817702621
rumen protozoal diversity in the spanish ibex (capra pyrenaica hispanica) as compared with domestic goats (capra hircus).rumen protozoal diversity in the spanish ibex (si) was studied in males (n=4), females (n=7) and young (n=4) from the maestrazgo (spain) and contrasted with domestic goats (n=3; dg) of the same region. there were no differences among si types in protozoal concentration or in the number of protozoal species. only protozoa from the genus entodinium were observed in si (seven species), the highest numbers corresponding to e. damae, e. ovibos and e. parvum. dg harboured threefold more species than s ...200918929470
epidemiological surveillance of bluetongue virus serotypes 1, 4 and 8 in spanish ibex (capra pyrenaica hispanica) in southern spain.a cross-sectional study was carried out to assess the prevalence and circulation of bluetongue virus (btv) in spanish ibexes (capra pyrenaica hispanica). a total of 770 sera samples, 380 blood samples and 34 spleen samples were collected between 2006 and 2009 in andalusia (southern spain), a region and time period with a wide circulation of btv in livestock. thirty-one out of 770 (4.0%; ci(95%): 2.6-5.4) sera samples analyzed by elisa showed antibodies against btv. twenty-four out of 31 seroposi ...201021111545
prevalence of toxoplasma gondii and neospora caninum antibodies in spanish ibex (capra pyrenaica hispanica).a cross-sectional study was carried out on spanish ibex populations in southern spain to assess the seroprevalence of toxoplasma gondii and neospora caninum and to investigate the risk factors associated with these infections. using the modified agglutination test, the seroprevalence to t. gondii was 27.5% (146/531; ci(95%), 23.7-31.3), and this seropositivity significantly increased with age. among adults, statistically significant differences were observed between geographical locations and ov ...201021159529
applicability of major histocompatibility complex drb1 alleles as markers to detect vertebrate hybridization: a case study from iberian ibex × domestic goat in southern spain.hybridization between closely related wild and domestic species is of great concern because it can alter the evolutionary integrity of the affected populations. the high allelic variability of major histocompatibility complex (mhc) loci usually excludes them from being used in studies to detect hybridization events. however, if a) the parental species don't share alleles, and b) one of the parental species possesses an exceptionally low number of alleles (to facilitate analysis), then even mhc l ...201223006678
seroprevalence and risk factors associated to mycobacterium bovis in wild artiodactyl species from southern spain, 2006-2010.the control of bovine tuberculosis (btb) is at a critical point in the last stage of eradication in livestock. wildlife species recently have emerged infected with tb in europe, particularly ungulates in the iberian peninsula. epidemiological information regarding tb in wild ungulates including affected species, prevalence, associated risk factors and appropriate diagnostic methods need to be obtained in these countries. a cross-sectional study was carried out on wild artiodactyl species, includ ...201222523563
management of a caseous lymphadenitis outbreak in a new iberian ibex (capra pyrenaica) stock reservoir.in 2010, an iberian ibex (capra pyrenaica hispanica) stock reservoir was established for conservation purposes in north-eastern spain. eighteen ibexes were captured in the wild and housed in a 17 hectare enclosure. once in captivity, a caseous lymphadenitis (cla) outbreak occurred and ibex handlings were carried out at six-month intervals between 2010 and 2013 to perform health examinations and sampling. treatment with a bacterin-based autovaccine and penicillin g benzatine was added during the ...201425492129
shedding of clostridium difficile pcr ribotype 078 by zoo animals, and report of an unstable metronidazole-resistant isolate from a zebra foal (equus quagga burchellii).clostridium difficile is an emerging and potentially zoonotic pathogen, but its prevalence in most animal species, including exhibition animals, is currently unknown. in this study we assessed the prevalence of faecal shedding of c. difficile by zoo animals, and determined the ribotype, toxin profile and antimicrobial susceptibility of recovered isolates. a total of 200 samples from 40 animal species (36.5% of which came from plains zebra, equus quagga burchellii) were analysed. c. difficile was ...201424467928
the role of wild ruminants as reservoirs of besnoitia besnoiti infection in cattle.bovine besnoitiosis, a parasitic disease caused by besnoitia besnoiti, has been reported mainly in beef cattle raised under extensive pastoral systems and is considered to be re-emerging in western europe. horizontal transmission probably occurs either by means of blood sucking arthropods or as a consequence of direct contact between infected and non-infected cattle. however, the role that wild ruminants (e.g., red deer (cervus elaphus) and roe deer (capreolus capreolus)) may play in the parasit ...201627198769
streptococcus caprae sp. nov., isolated from iberian ibex (capra pyrenaica hispanica).biochemical and molecular genetic studies were performed on a novel gram-stain-positive, catalase-negative, coccus-shaped organism isolated from tonsil samples of two iberian ibexes. the micro-organism was identified as a streptococcal species based on its cellular, morphological and biochemical characteristics. 16s rrna gene sequence comparison studies confirmed its identification as a member of the genus streptococcus, but the organism did not correspond to any species of this genus. the neare ...201626486442
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