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formation of sulfhydryl groups in the walls of eimeria stiedai and e. tenella oocysts subjected to in vitro excystation.eimeria stiedai or eimeria tenella oocysts were incubated in aqueous cysteine hydrochloride (cyshcl) under carbon dioxide (co2), aqueous cyshcl under air, water under co2 or water under air, and analyzed for sulfhydryl (-sh) groups. the cyshcl-co2 treatment produced more -sh groups than the other treatments and was effective in allowing activation of intact and sodium hypochlorite (naocl)-treated e. stiedai oocysts as well as naocl-treated e. tenella oocysts. the co2-cyshcl complex may act direc ...1976177746
partial purification and some properties of glucose 6-phosphate dehydrogenase from eimeria stiedai (lindemann, 1865) kisskalt & hartmann, 1907 (protozoa: coccidia). 19767427
diagnosis of encephalitozoonosis in experimentally infected rabbits by intradermal and immunofluorescence tests.the indirect immunofluorescence antibody test was performed on serial blood samples from eight young new zealand white rabbits with experimental encephalitozoonosis. the test showed seroconversion in six of the eight infected rabbits by the 8th day after inoculation and in all rabbits by the 15th day. antibody titers reached a peak by about the 36th day after inoculation and remained significantly elevated until the termination of the experiment at 84 days after inoculation. none of four sham-in ...1977323570
the levels of total protein and protein fractions in the serum of rabbits infected with eimeria stiedai.the total protein levels in rabbits infected with eimeria stiedai together with the alterations in the different protein fractions over a period of 35 days were studied. it was observed that from the second week of infection onwards a progressive increase took place in total proteins; the electrophoretic study revealed that the hyperproteinemia is mainly due to the increase in gamma-globulins accompanied by a decrease in the concentration of albumin and a increase in alpha and beta-globulins. th ...19863813422
further studies on the properties of glucose 6-phosphate dehydrogenase from the coccidium eimeria stiedai (protozoa).1. glucose 6-phosphate dehydrogenase from eimeria stiedai does not reduce nad or any of its analogs tested. it does reduce nadp and its thionicotinamide and 3-acetylpyridine analogs. 2. it will accept d-glucose as substrate, but not 2-deoxy-d-glucose, glucose 1-phosphate, or 2-deoxy-d-glucose 6-phosphate. 3. its response to a number of compounds that activate or inhibit the enzyme from other organisms has been determined. 4. the molecular weight is ca. 240,000 by gel chromatography, and only one ...1978318345
the effects of selected gases on excystation of coccidian oocysts.the mechanism of co2 action in changing coccidian oocyst wall permeability was indirectly studied by substituting no, no2, n2o, h2s, so2, ch4, nh3, and 8m urea in place of co2 in an established excystation procedure. changes in oocyst wall permeability of eimeria stiedai, e. bovis, and e. tenella were determined by incubation in test gases and cysteine hcl followed by attempted activation of sporozoites by trypsin and bile and staining of intraoocyst components with methylene blue. the gases ch4 ...19761263026
effects of methylglyoxal bis(guanylhydrazone) and two phenylated analogues on s-adenosylmethionine decarboxylase activity from eimeria stiedai (apicomplexa).1. activity of s-adenosylmethionine decarboxylase, one of the rate-limiting enzymes of polyamine biosynthesis, was determined in oocysts of eimeria stiedai, a coccidian parasite of the rabbit. 2. several properties of the enzyme were compared to the mammalian enzyme. it showed considerably less substrate affinity than the analog enzyme from the rabbit. 3. the e. stiedai enzyme showed a low sensitivity to methylglyoxal bis(guanylhydrazone), a frequently used inhibitor of the enzyme in mammals, an ...19873665433
chemotherapy and chemoprophylaxis of hepatic coccidiosis with sulphadimethoxine and pyrimethamine.the activity of a mixture of sulphadimethoxine and pyrimethamine (10:3) as prophylactic medication and prophylactic and therapeutic medication was studied in rabbits experimentally infected with eimeria stiedai. the haematocrit index (packed cell volume) and haemoglobin levels were studied for assessment of drug toxicity. the activity in serum of aspartate aminotransferase (ast) and alanine aminotransferase, alkaline phosphatase and gamma-glutamyltranspeptidase were studied as indicators of hepa ...19862876468
experimental transmission of eimeria stiedai to the hare. 19761020727
diagnosis of experimental encephalitozoonosis in rabbits by complement fixation.a complement-fixation (cf) test has been developed for detection of experimental encephalitozoonosis in rabbits. the antigen consisted of disrupted homogenates of encephalitozoon cuniculi spores grown in and released from rabbit choroid plexus tissue culture cells. the test was sensitive and capable of detecting experimental encephalitozoonosis in rabbits as early as 15 days after intracerebral infection. the test was specific for infected animals, and no cross-reactivity was demonstrated betwee ...1977864288
pharmacokinetics, hepatic biotransformation and biliary and urinary excretion of bromosulfophthalein (bsp) in an experimental liver disease mimicking biliary cirrhosis.we studied the hepatic handling of bromosulfophthalein in healthy rabbits with hepatic coccidiosis 28 days after an experimental infection with sporulated oocysts of eimeria stiedai, an experimental model of liver disease histopathologically resembling primary biliary cirrhosis in man. a pharmacokinetic study of the results was performed following a multicompartmental model with 7 transfer constants to describe the physiological disposition of the dye. the study showed that the plasma disappeara ...19902384120
prevalence and pathological study on rabbit hepatic coccidiosis in taiwan.five breeds of rabbits, which included the new zealand, californian, spot, rex and angora rabbit, were found from a survey of 1,152 rabbits in taiwan. the prevalence of coccidia in young rabbits (weaning-2 months old) was 95% to 100%. adult female rabbits usually acted as carriers within the farm and transmitted the parasite to young rabbits, which caused severe infection with clinical signs and even death. parasitism of hepatic coccidia (eimeria stiedai) in the rabbit led to severe mortality. n ...19911815262
effects of saturated sodium chloride solution on coccidial oocysts.using the ability to sporulate as a measure of viability, the effects of exposure of unsporulated oocysts of 10 species of coccidia of chickens, rabbits and cattle to saturated nacl solution has been studied. although appreciable deformation and collapse of the oocyst occurred after 1-2 days contact, the effect was reversible after washing free from salt and incubating. some reduction in ability to sporulate following several days contact with saturated salt was noted in most species, although n ...1978756524
progress in taxonomy of the apicomplexan protozoa.in 1987, 4516 species and 339 genera of the phylum apicomplexa had been named. they consisted of the gregarines (subclass gregarinasida) (1624 named species and 231 named genera), the hemogregarines (family haemogregarinidae) (399 species and 4 genera), the eimeriorins (order eimeriorida) (1771 species and 43 genera), the hemospororids (order haemospororida) (444 species and 9 genera), the piroplasmids (order piroplasmorida) (173 species and 20 genera), and a few others (105 species and 32 gener ...19883143826
preventive treatment of rabbit coccidiosis with alpha-difluoromethylornithine.the effect of alpha-difluoromethylornithine (dfmo) administered in drinking water (0.2 and 0.5%) against liver and intestinal coccidioses in experimentally infected rabbits was studied. zootechnical and clinical parameters were used to assess the efficacy of the compound. in both coccidioses the lower dose did not show any important effect. the higher dosage (0.5%) however, reduced the lesions induced by coccidia to an important extent. the animals infected and treated with 0.5% dfmo showed simi ...19883145642
[motility of sporozoites of eimeria stiedai (protozoa, sporozoa)]. 19724669289
[excystation of sporozoites in eimeria stiedai]. 19724674936
eimeria stiedae: weight, oocyst output, and hepatic function of rabbits with graded infections. 1979510445
the effect of the host's age on the pathology of eimeria stiedai infection in rabbits.the pathology of experimental eimeria stiedai infection in 2- and 4-month-old rabbits raised coccidia-free was studied. over 50 days of infection, the serum activities of aspartate (ast) and alanine (alt) aminotransferases, alkaline phosphatase (ap) and gamma-glutamyltranspeptidase (ggtp) were analyzed as indicators of hepatic lesions and total serum globulins with respect to the immune response of the host; parasite development was followed by oocyst output; weight gain, food intake and relativ ...19872884769
improved method for high-yield excystation and purification of infective sporozoites of eimeria spp.this report describes a new, gentle procedure for rapid and efficient excystation of large numbers of infective sporozoites of eimeria vermiformis and eimeria stiedai. excysted sporozoites are purified using modifications of a previously described ion-exchange chromatography method. the procedure avoids physical breakage of oocysts and results in greater than 70% recovery of the sporozoites present as sporulated oocysts (i.e. 5-6 sporozoites per sporulated oocyst). the recovered sporozoites are ...19883058952
identification of virus-like particles in eimeria stiedae.when nucleic acid samples purified from sporozoites of eimeria stiedae were analyzed by agarose gel electrophoresis, an ethidium-stainable band with an apparent electrophoretic mobility of 6.5 kb was consistently observed. the band was readily degradable upon rnase treatment, and its susceptibility towards ribonuclease a on a decreasing ionic strength was suggestive of double-stranded rna (dsrna). electron microscopy revealed spherical, probably icosahedral, virus-like particles (vlp) with a dia ...19892797059
ultrastructure of microgametogenesis of eimeria stiedai in rabbits (oryctolagus cuniculus). 19882978214
efficacy of diclazuril in the prevention and cure of intestinal and hepatic coccidiosis in rabbits.the efficacy of diclazuril against intestinal and hepatic coccidiosis was studied in artificially infected rabbits. prophylaxis against intestinal coccidiosis was evaluated using a mixed infection of eimeria intestinalis, eimeria magna and eimeria perforans. continuous medication in the feed at 1 p.p.m. was 100% effective in reducing oocyst output and faecal scores, and weight gain and feed efficiency were normal. hepatic coccidiosis induced by eimeria stiedai was prevented at 0.5 and 1 p.p.m. a ...19892773264
[alteration of enzyme activities in serum of eimeria stiedai infected rabbits (author's transl)].in experimental investigations on eimeria stiedai infected rabbits, serum enzymatic studies have been carried out in correlation with the examination of parasitological and pathological parameters. the rabbits were orally infected with a single dose of either 100,000 or 250,000 sporulated oocysts. increase of the activity of the sorbit dehydrogenase (sdh), glutamate oxalate transaminase (got), glutamate pyruvate transaminase (gpt) and glutamate dehydrogenase (gldh) could be found first between 3 ...1978735305
[the internal development and schizogony in cultured cells of eimeria stiedai (lindemann 1865) kisskalt and hartmann 1907)]. 19744218972
[transmission of eimeria stiedai from the rabbit (oryctolagus cuniculus) to the hare (lepus europaeus)]. 1979543475
eimeria stiedai in rabbits: the presence of an oocyst residuum.observations on 5 different isolates of eimeria stiedai from pure infections in laboratory-reared rabbits have confirmed the presence of an oocyst residuum. this structure was seen in 89--100% of the oocytes in cultures prepared both from the liver and from the faeces at different times during the patent period. the residuum consisted of 1--7 or more granules situated centrally in the oocyst and partially concealed by 4 sporocysts. the differential diagnosis of e. stiedai and e. coecicola is dis ...1977593726
results of blood transfusions from donor rabbits infected with eimeria stiedai to recipient coccidiafree rabbits. 19744838481
structure and respiration of sporulating eimeria stiedae and e. tenella oocysts. 19694894400
eimeria stiedai: delayed hypersensitivity response in rabbit coccidiosis. 1976130248
life cycle of eimeria stiedai. 19724669279
[resistance of eimeria stiedai oocysts to roentgen and gamma radiation]. 19724677458
studies on the storage polysaccharides of the coccidia eimeria bovis and eimeria stiedai. 19744138525
some peculiarities of the fine structure of merozoites of eimeria stiedai. 19715163214
[action of ultracentrifugation on the morphological characteristics of eimeria stiedai sporonts]. 19724624957
[sporogony of eimeria stiedai (protozoa, sporozoa)]. 19715152847
[endoparasite infestation of the stomach and intestinal tract of feral rabbits from the leipzig region].investigations on the infestation with endoparasites in stomach and gut of feral rabbits from the region of leipzig. during the hunting periods in the years 1976 till 1979 the viscera of 122 wild rabbits shot in two different biotopes in the area of leipzig were examined for endoparasites. 63.11% harboured ctenotaenia ctenoides and cittotaenia denticulata and 8.19% cysticercus pisiformis. graphidium strigosum was demonstrated in 36.88%, trichostrongylus retortaeformis in 82.78%, passalurus ambig ...19846465615
eimeria stiedae: cytochemical identification of acid and alkaline phosphatases, carboxylic ester hydrolases, and succinate, lactate, and glucose-6-phosphate dehydrogenases in endogenous stages from rabbit tissues. 19685701763
eimeria stiedai in rabbits: the demonstration of responses to chemotherapy.the activity of sulphaquinoxaline, robenidine, methyl benzoquate, clopidol and a mixture of methyl benzoquate and clopidol (lerbek; dow), was studied in rabbits infected with hepatic coccidiosis due to eimeria stiedai. growth inhibition, oocyst production and the activity in the serum of glutamate dehydrogenase and gamma glutamyltransferase were studied as indicators of parasite development. only sulphaquinoxaline and lerbek gave satisfactory control of this parasite. the latter formulation was ...19836132429
parenteral infection experiments with eimeria stiedai. 19695800627
large-scale management systems and parasite populations: coccidia in rabbits.broiler rabbit production has become an important branch of animal protein and fur production, not only in the traditionally rabbit breeding and consuming countries, but recently in many other countries of the world. the profitability of the rabbit industry is dependent primarily on the good feed conversion. aspects of this include factors such as morbidity and mortality of infections, adequate knowledge and fulfilment of both accommodation and nutritional requirements, appropriate breeding syst ...19826891527
effect of ultra-violet irradiation on the oocystic zygotes of eimeria stiedae. 19817197701
virus-like particles in eimeria nieschulzi are associated with multiple rna segments.rna preparations from sporulated oocysts of eimeria nieschulzi were found to contain 2 double-stranded rna segments of 5.0 kb and 5.7 kb that were not present in other species of eimeria. treatment of crude lysates with rnase a revealed that in addition to these two segments, 3 other segments of 0.57 kb, 0.72 kb and 11.5 kb were protected from digestion, suggesting that they were enclosed within particles. virus-like particles with a diameter of approximately 39 nm were purified by caesium chlor ...19948008024
enzymes of coccidia: purification and properties of l-lactate dehydrogenase from eimeria stiedae. 19724346146
the ultrastructural development of the macrogamete of eimeria stiedai. 19807444091
[the endogenous developmental cycle of eimeria stiedai (lindemann, 1865) kisskalt and hartmann, 1907]. 19705474491
identification of carbohydrates on eimeria stiedai sporozoites and their role in the invasion of cultured cells in vitro.the carbohydrates present on eimeria stiedai sporozoites and their functional role in the process of invasion of host cells were examined. lectin-binding sites on the surface of sporozoites were detected by means of peroxidase-conjugated lectins. sporozoites showed specific binding with uea-i and pna lectins, which bind l-fucose and d-galactose, respectively. exposure of sporozoites to 100 microg/ml uea-i significantly reduced their ability to invade primary rabbit liver biliary epithelial cells ...199910030752
eimeria stiedae: cytochemical identification of enzymes and lipids in sporozoites and endogenous stages. 19705414955
tissue residues of some sulphonamides in normal and eimeria stiedai infected rabbits.tissue residues of sulphadiazine (sdz), sulphadimidine (sdd) and sulphquinoxaline (sq) were studied in healthy and e. stiedai infected rabbits following oral administration of 0.5 g/l drinking water for 5 days. the solid-phase extraction and hplc was used to determine the concentration of the three sulphonamides in a single tissue sample. sdz was detected in the liver and kidney in concentrations below the tolerance levels at day 5 and no residues could be detected at day 7 after drug withdrawal ...199910481374
acid-base disturbances in the rabbit during experimental hepatic parasitosis.acid-base disturbances were examined during experimentally induced infection in the rabbit with eimeria stiedai, a parasite that profoundly modifies liver morphology and physiology, resulting in anatomical and functional alterations similar to those appearing in different human hepatic diseases. over 28 days of infection, bicarbonate and lactate concentrations, partial pressures of o2 and co2, and ph values were determined in the blood and bile of infected animals and compared with the values ob ...19968832733
development of eimeria stiedae in a non-specific host.infection with e. stiedae was established in immunocompromised mice given dexamethasone for ten days prior to the infection and for two weeks later. the infection was similar to that of rabbits as regard the pathological and clinical picture.19938482887
life cycle of eimeria stiedae. 19705428207
factors influencing the fecal egg and oocyst counts of parasites of wild european rabbits oryctolagus cuniculus (l.) in southern western australia.abundance of intestinal parasites was monitored by fecal egg and oocyst counts for samples of wild rabbits oryctolagus cuniculus with different levels of imposed female sterility from 12 populations in southwestern australia. differences in egg counts of trichostrongylus retortaeformis between seasons and age groups were dependent on the sex of the host. pregnancy may have been responsible for these differences because egg counts were consistently higher in intact females than in females surgica ...199910577712
trail antigen in eimeria stiedai sporozoites associated with a thrombospondin-related motif and the entry of cultured cells.in order to examine the antigenic similarity and specificity of the trail antigen of eimeria stiedai and etp 100, a microneme protein of eimeria tenella, monoclonal antibodies to the trail antigen of e. stiedai sporozoites were selected by an indirect immunofluorescent antibody method. the monoclonal antibody of one clone, 3d10, reacted with the anterior portion of non-fixed sporozoites. by immunoblotting, the monoclonal antibody was found to react with a 100 kda antigen of e. stiedai sporozoite ...200111511415
identification and the role of soluble antigens detected in bile from eimeria stiedai-infected rabbits.antibodies against eimeria stiedai sporozoites and merozoites were detected in the sera of rabbits immunized with bile obtained from infected rabbits on the 15th day post-infection. the trails made by gliding sporozoites were also detected by the sera. after penetration into the host cell, an antibody-binding region was observed on the parasitophorous vacuole membranes of the parasites. rabbits administered a combination of the bile and cholera toxin shed fewer oocysts in the feces after infecti ...200111318557
identification of carbohydrates on eimeria stiedai sporozoites and their role in invasion of cultured cells in vitro.invasion specificity of eimeria stiedai sporozoites in cultured cells was examined. intracellular sporozoites were observed in hepatobiliary epithelial cells, as early as 3 hours post inoculation (p.i.) but the infection rate was monitored for 6 hours. no intracellular parasites were found in rabbit parenchymal hepatocytes and rabbit kidney cells, even on prolonged culturing. in the hepatobiliary epithelial cells inoculated with fixed sporozoites, no intracellular parasite were found. sporozoite ...199810622634
eimeria stiedai merozoite 49-kda soluble antigen induces protection against infection.a soluble antigen isolated from eimeria stiedai merozoites with a molecular mass of 49 kda was detected in the bile of infected rabbits. rabbits immunized with the antigen shed a lower number of oocysts than did nonimmunized rabbits postchallenge (p.c.). the immunized rabbits showed a marked and transient increase of alanine-aminotransferase (alt) activity on day 8 p.c. the blood indocyanine green (icg) clearance and r-glutamyltransferase (ggt) activity showed no change throughout the experiment ...200312880268
reduction of the infectivity of toxoplasma gondii and eimeria stiedai sporozoites by treatment with bovine lactoferricin.sporozoites of toxoplasma gondii preincubated with lactoferricin showed decreased activity in penetration of mouse embryonal cells. mice inoculated with 10(5) sporozoites preincubated with lactoferricin showed a higher survival rate than those inoculated with the same number of untreated sporozoites. likewise, sporozoites of eimeria stiedai preincubated with lactoferricin also showed decreased activity in penetration of rabbit hepatobiliary cells. rabbits inoculated with 10(5) sporozoites preinc ...200111258458
eimeria stiedae: experimental infection in rabbits and the effect of treatment with toltrazuril and ivermectin.the aim of this study was to investigate the clinical, haematological, biochemical, lipid peroxidation, ultrasonographic and pathologic findings in hepatic coccidiosis induced by eimeria stiedae in rabbits, and also to compare the treatment effects of both toltrazuril and ivermectin separately and in combination. in this study, 56 rabbits were divided into eight groups. the first group was designated as healthy control group. rabbits were infected with 40.000 sporulated oocysts of e. stiedae. gr ...200818321485
the specificity of the sabin-feldman dye test with reference to protozoal infections.infections of eimeria stiedae in normal and splenectomized rabbits and of leishmania enriettii in normal rabbits and guinea-pigs and in splenectomized rabbits did not produce dye test antibodies. guinea-pigs injected with atoxoplasma sp. and rabbits immunized with cultures of crithidia fasciculata were found to be negative for dye test antibodies. mice which had been infected with trypanosoma cruzi did not show dye test antibodies. all these animals, except one guinea-pig infected with leishmani ...196014412642
the effects of age and temperature on the survival of oocysts of eimeria stiedae. 195813583403
serological reactions in eimeria stiedae infection of the rabbit. 195913653731
relationship between liver disorders and protection against eimeria stiedai infection in rabbits immunized with soluble antigens from the bile of infected rabbits.soluble antigens exist in the bile of rabbits infected with eimeria stiedai (e. stiedai) in the acute phase, and rabbits immunized with the antigens show resistance against the infection. in this study, the liver function of rabbits immunized either with the soluble antigens or pbs were examined following the parasite challenge. rabbits immunized with pbs shed a number of oocysts and showed an increase in r-glutamyltransferase (ggt) activity and a decrease in blood indocyanine green (icg) cleara ...200312531300
the effect of single and concomitant pathogen infections on condition and fecundity of the wild rabbit (oryctolagus cuniculus).we assessed the effect of two pathogens (myxoma virus and eimeria stiedae) and five macroparasites (gastrointestinal helminth species) of the wild rabbit (oryctolagus cuniculus) upon total host body mass and abdominal fat level. additionally, we assessed the effects of these organisms on the number of foetuses in adult females during the peak breeding period. both mass of abdominal fat and total body mass of the rabbit were negatively associated with myxoma virus infection and increasing helmint ...200516051247
effect of subcutaneously administered diclazuril on the output of eimeria species oocysts by experimentally infected rabbits.the effect of subcutaneously injected diclazuril on the output of eimeria species oocysts was studied in experimentally infected rabbits. diclazuril was administered either prophylactically at 0.5, 1 or 2 mg/kg bodyweight two days before each rabbit was inoculated with 20,000 oocysts of a mixed-species field isolate of eimeria or therapeutically at 1, 2 or 4 mg/kg bodyweight five days after they were inoculated. the prophylactic treatments significantly reduced (p<0.05) the output of oocysts in ...200818245747
protective effect of eimeria stiedae coproantigen against hepatic coccidiosis in rabbits.immunization of rabbits against hepatic coccidiosis was tried. the animals were immunized twice with eimeria stiedae coproantigen in freund's adjuvant with two week intervals. the rabbits were challenged orally with sporulated e. stiedae oocysts one week post last injection. the protection was assessed by number of oocysts output, number of focal liver lesions, clinical sings and antibody response. the immunization resulted in 70% protection from infection and decline in oocysts count. high leve ...200516083069
autoantibody production in rabbits. iii. the effect of infection with eimeria stiedae and its relation to natural antibody. 196313965167
efficacy of garlic extract on hepatic coccidiosis in infected rabbits (oryctolagus cuniculus): histological and biochemical studies.the rabbits were divided into three groups, of 12 rabbits each. g1 was the (non-infected non-treated) as control, g2 was the (infected-non treated), and g3 was the (infected and treated) rabbits. each rabbit in the infected groups were given (10(3)) sporuleted oocysts of eimeria stiedae per rabbit after forty five days exactly. faecal sample of rabbits from each group were examined each day post infection till oocysts appeared in faeces. the treatment was given by using suitable dosage of garlic ...200718383795
oxygen uptake of liver slices from rabbits infected with eimeria stiedae. 195813554583
the inhibitory effect of acridine on the sporogony of a coccidium (eimeria stiedae).the development or ripening of the oocyst of the coccidium of the rabbit is prevented by acridine hydrochloride provided that the cysts are exposed to the action of the chemical before development has started. after sporoblasts are formed acridine does not prevent further development. many other substances, some of them known to be active against certain protozoan parasites, have no influence on the ripening of the oocysts of the coccidium.192419868914
estimating relative pollution of the environment with oocysts of eimeria stiedae. 195413212542
the effect of sulfaquinoxaline on the course of eimeria stiedae infections in the domestic rabbit. 195413210367
prevalence, pathology and risk factors for coccidiosis in domestic rabbits (oryctolagus cuniculus) in selected regions in kenya.the potential for rabbit production in kenya is high. however, high morbidity and mortality of domestic rabbits were reported.201425322770
simultaneous identification of three highly pathogenic eimeria species in rabbits using a multiplex pcr diagnostic assay based on its1-5.8s rrna-its2 fragments.eimeria stiedai, e. intestinalis, and e. flavescens are highly pathogenic in rabbits, especially rabbits younger than 3 months. in this study, the complete its1-5.8s rrna-its2 sequences of six rabbit eimeria species, e. stiedai, e. intestinalis, e. flavescens, e. media, e. magna, and e. irresidua, were cloned with universal primers for the genus eimeria and genomic dna of ly and kf isolates as templates. these results revealed that both its1 and its2 sequences were specific to each eimeria speci ...201323246036
observations on oocyst development of eimeria stiedai in rabbits.the formation of the oocyst wall was examined in eimeria stiedai in the bile duct epithelium of the rabbit and was found to follow the general eimerian pattern. however from the beginning of the formation of the outer layer of the oocyst wall the parasite was surrounded by a rarely reported veil membrane. cell damage of the bile ducts at the gamogony stage of parasite development is depicted.201425119371
some heterocyclic thione derivatives exhibit anticoccidial activity by inhibiting glycosidases.coccidiosis is one of the most common parasitic diseases affecting many species of domestic animals. this disease has a major economic significance and the search for new compounds having anticoccidial activity is of great importance. in this article, different levels of protection from coccidian infection by eimeria stiedae were developed in rabbits by treatment with compounds incorporating the skeleton of thiourea. these compounds include 4,5-diphenylimidazole-2-thione (1), 4,5-diphenyl-1,2,4- ...201223074703
molecular analysis of single oocyst of eimeria by whole genome amplification (wga) based nested pcr.pcr-based molecular tools are widely used for the identification and characterization of protozoa. here we report the molecular analysis of eimeria species using combined methods of whole genome amplification (wga) and nested pcr. single oocyst of eimeria stiedai or eimeriamedia was directly used for random amplification of the genomic dna with either primer extension preamplification (pep) or multiple displacement amplification (mda), and then the wga product was used as template in nested pcr ...201424996066
evaluation of some coagulation parameters in hepatic coccidiosis experimentally induced with eimeria stiedai in rabbits.the objective of this study was to evaluate some coagulation parameters in hepatic coccidiosis experimentally induced with eimeria stiedai in rabbits. fourteen healthy new zealand rabbits were equally divided into two groups. one group received no treatment, the other group was orally inoculated with 40 000 sporulated oocysts of e. stiedai in a 1 ml inoculum using a catheter. at day 24 after inoculation, blood samples were collected into sodium citrate-containing tubes to evaluate some coagulati ...200616629990
toxoplasma gondii coinfection with diseases and parasites in wild rabbits in scotland.in wild rabbits (oryctolagus cuniculus) on an estate in perthshire, central scotland, the seroprevalence of toxoplasma gondii was 18/548 (3·3%). the wild rabbit could be a t. gondii reservoir and it has potential value as a sentinel of t. gondii in environmental substrates. toxoplasma gondii was associated with female sex (p < 0·001) and with relatively heavy infections by eimeria stiedae (p = 0·036). it was not associated with the intensity of coccidial oocysts, the severity of myxomatosis caus ...201526144268
potential vaccine targets against rabbit coccidiosis by immunoproteomic analysis.the aim of this study was to identify antigens for a vaccine or drug target to control rabbit coccidiosis. a combination of 2-dimensional electrophoresis, immunoblotting, and mass spectrometric analysis were used to identify novel antigens from the sporozoites of eimeria stiedae. protein spots were recognized by the sera of new zealand rabbits infected artificially with e. stiedae. the proteins were characterized by matrix-assisted laser desorption ionization time of flight mass spectrometry (ma ...201728285502
study on impression smears of hepatic coccidiosis in rabbits.hepatic coccidiosis is a contagious and lethal disease condition in rabbits. the disease was recorded in six rabbits suffering with watery diarrhoea. clinically, affected rabbits showed decreased growth rate, anorexia, debilitation, diarrhea and rough hair coat. examination of the faecal samples revealed the presence of unsporulated oocysts of eimeria spp. after sporulation eimeria stiedae oocysts were identified. postmortem examination revealed hepatomegaly with presence of discrete yellowish-w ...201627605807
complete genome sequence and evolution analysis of eimeria stiedai rna virus 1, a novel member of the family totiviridae.eimeria stiedai (e. stiedai) is a coccidian that infects the liver of the domestic rabbit and may cause severe hepatic coccidiosis. virus-like particles in e. stiedai were discovered by revets et al. however, the complete genome sequence of the e. stiedai virus has yet to be determined. a novel virus was isolated from e. stiedai in the present study. the complete genome sequence of the e. stiedai virus was 6219 bp in length and contained two open reading frames (orfs) with a tetranucleotide over ...201627578037
molecular diagnosis of eimeria stiedae in hepatic tissue of experimentally infected rabbits.the early detection of eimeria stiedae in the hepatic tissue of experimentally infected rabbits was investigated using molecular assay. forty 6-week-old male new zealand rabbits were divided into two groups. group a (30 animals) was infected with 2.5 × 10(4) sporulated oocysts of e. stiedae per animal on day 0 and group b (10 animals) was used as the uninfected controls. three animals from group a and one from group b were sacrificed at 0, 3, 6, 9, 12, 15, 18, 21, 24 and 27 days post infection ( ...201627423971
pathological and ultrastructural observations and liver function analysis of eimeria stiedai-infected rabbits.to study the pathogenicity of eimeria stiedai, sporulated oocysts were given orally to coccidian-free two-month-old new zealand rabbits(1000±20g). after 30days, blood samples from the rabbit hearts were collected for routine blood tests, liver functions and four characteristics of blood coagulation. additionally, specimens of the liver, bile duct and duodenum were collected to observe the changes in pathology and ultrastructure. e. stiedai severely restricted the growth and development of rabbit ...201627198796
anti-coccidial effect of commiphora molmol in the domestic rabbit (oryctolagus cuniculus domesticus l.).this study investigated the effect of oleo-gum-resin of commiphora monlmol engler (family: burceraceae) known as myrrh and the commercial extract known as mirazid as a treatment against hepatic coccidiosis induced by the parasite eimeria stiedae in domestic rabbits. rabbits were infected with 1000 parasite sporulated oocysts and subjected to two treatment regimens, using crude myrrh suspension and the oleo-resin extract, mirazid, each administered at 500 mg/kg rabbit body weight. treatments of i ...201021268535
histopathological features of capillaria hepatica infection in laboratory rabbits.capillaria hepatica is a nematode parasite of wild rodents and other mammals. adult worms inhabit the liver. recently, during the necropsy examination of a group of 160 rabbits from a commercial supplier, firm pale or cystic areas (1-5 mm) were noted on the liver in thirteen animals. on further investigation, these animals were found to be infected with c. hepatica. the histopathological features of the infection in the rabbit are described for the first time and diagnostic features recorded. le ...200919581239
garlic and hepatic coccidiosis: prophylaxis or treatment?a study was conducted to investigate the protective and therapeutic effects of crude garlic (allium sativum) against experimental infection with eimeria stiedae in rabbits. forty rabbits were divided into four groups of ten rabbits each: a healthy control group (hc); a challenged-garlic-protected group (cgp) which received a daily dose of 0.5 g/kg body weight (bwt) crude garlic for five successive days before challenge with e. stiedae; a challenged-garlic-treated group (cgt) which was treated wi ...201020473715
[gastrointestinal parasites of rabbits in coproscopic investigations].parasitological screening was carried out in a breeding herd of the new zealand white rabbit breed in 2004.200617432628
comparison of toltrazuril and sulphadimethoxine in the treatment of intestinal coccidiosis in pet rabbits.treatment of intestinal coccidiosis was studied in domestic pet rabbits. in 45 rabbits aged four months or more, coccidial oocysts were observed in the faeces of 35 rabbits at a mean density of 806 opg (range 50 to 6800 opg). eimeria magna was the dominant species, with eimeria media and eimeria intestinalis also being common. the presence of the hepatic species eimeria stiedae was not recorded. a single oral dose of 2.5 mg/kg or 5.0 mg/kg toltrazuril, or a single oral dose of 50 mg/kg sulphadim ...201020729515
systemic inflammatory response indicators in rabbits (oryctolagus cuniculus) experimentally infected with sporulated oocysts of eimeria stiedai (apicomplexa: eimeriidae).hemograms and acute-phase proteins in adult male new zealand white rabbits that had been experimentally infected orally with sporulated oocysts of eimeria stiedai were evaluated over a 28-day period. fifty animals were used, divided into two groups: group a infected with 1 +ù 10(4) sporulated oocysts of e. stiedai and group b inoculated with distilled water. on the seventh day after infection, the infected animals presented anemia and leukocytosis with neutrophilia and monocytosis. protein fract ...201121722486
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