pantoea vagans sp. nov., pantoea eucalypti sp. nov., pantoea deleyi sp. nov. and pantoea anthophila sp. nov.bacteria isolated from eucalyptus leaves and shoots showing symptoms of blight and die-back collected in uganda, uruguay and argentina and from maize displaying brown stalk rot symptoms in south africa were tentatively placed in the genus pantoea on the basis of phenotypic and biochemical tests. these isolates, together with two strains (lmg 2558 and lmg 2560) previously assigned to pantoea agglomerans based on protein electrophoregrams but later excluded from this species, were further investig ...200919620357
genomic and phenotypic characterization of a nonpigmented variant of pantoea vagans biocontrol strain c9-1 lacking the 530-kb megaplasmid ppag3.a 530-kb megaplasmid ppag3 contributing 10.8% of the total genome of pantoea vagans biocontrol strain c9-1 was sequenced. a rare nonpigmented variant c9-1w was obtained and shown to have lost ppag3, but retained all other plasmids (ppag1, ppag2). phenotypic characterization of the variant confirmed the function of several annotated genes that may influence ecological fitness and efficacy. metabolic profiling revealed important plasmid-based carbon utilization phenotypes. plasmid loss resulted in ...201020487014
mechanistically compatible mixtures of bacterial antagonists improve biological control of fire blight of pear.mixtures of biological control agents can be superior to individual agents in suppressing plant disease, providing enhanced efficacy and reliability from field to field relative to single biocontrol strains. nonetheless, the efficacy of combinations of pseudomonas fluorescens a506, a commercial biological control agent for fire blight of pear, and pantoea vagans strain c9-1 or pantoea agglomerans strain eh252 rarely exceeds that of individual strains. a506 suppresses growth of the pathogen on fl ...201120839962
control of fire blight by pseudomonas fluorescens a506 and pantoea vagans c9-1 applied as single strains and mixed inocula.the biological control agents pseudomonas fluorescens a506 and pantoea vagans c9-1 were evaluated individually and in combination for the suppression of fire blight of pear or apple in 10 field trials inoculated with the pathogen erwinia amylovora. the formulation of pathogen inoculum applied to blossoms influenced establishment of the pathogen and the efficacy of biological control. pantoea vagans c9-1 suppressed fire blight in all five trials in which the pathogen was applied as lyophilized ce ...201020839963
genome sequence of the biocontrol agent pantoea vagans strain c9-1.pantoea vagans is a gram-negative enterobacterial plant epiphyte of a broad range of plants. here we report the 4.89-mb genome sequence of p. vagans strain c9-1 (formerly pantoea agglomerans), which is commercially registered for biological control of fire blight, a disease of pear and apple trees caused by erwinia amylovora.201020952567
metabolic versatility and antibacterial metabolite biosynthesis are distinguishing genomic features of the fire blight antagonist pantoea vagans c9-1.pantoea vagans is a commercialized biological control agent used against the pome fruit bacterial disease fire blight, caused by erwinia amylovora. compared to other biocontrol agents, relatively little is currently known regarding pantoea genetics. better understanding of antagonist mechanisms of action and ecological fitness is critical to improving efficacy.201121789243
fire blight disease reactome: rna-seq transcriptional profile of apple host plant defense responses to erwinia amylovora pathogen infection.the molecular basis of resistance and susceptibility of host plants to fire blight, a major disease threat to pome fruit production globally, is largely unknown. rna-sequencing data from challenged and mock-inoculated flowers were analyzed to assess the susceptible response of apple to the fire blight pathogen erwinia amylovora. in presence of the pathogen 1,080 transcripts were differentially expressed at 48 h post inoculation. these included putative disease resistance, stress, pathogen relate ...201626883568
characterization of the biosynthetic operon for the antibacterial peptide herbicolin in pantoea vagans biocontrol strain c9-1 and incidence in pantoea species.pantoea vagans c9-1 is a biocontrol strain that produces at least two antibiotics inhibiting the growth of erwinia amylovora, the causal agent of fire blight disease of pear and apple. one antibiotic, herbicolin i, was purified from culture filtrates of p. vagans c9-1 and determined to be 2-amino-3-(oxirane-2,3-dicarboxamido)-propanoyl-valine, also known as n(ß)-epoxysuccinamoyl-dap-valine. a plasposon library was screened for mutants that had lost the ability to produce herbicolin i. it was sho ...201222504810
draft genome sequences of pantoea agglomerans and pantoea vagans isolates associated with termites.the genus pantoea incorporates many economically and clinically important species. the plant-associated species, pantoea agglomerans and pantoea vagans, are closely related and are often isolated from similar environments. plasmids conferring certain metabolic capabilities are also shared amongst these two species. the genomes of two isolates obtained from fungus-growing termites in south africa were sequenced, assembled and annotated. a high number of orthologous genes are conserved within and ...201626937267
analysis of the effect of high hydrostatic pressure treatment and enterocin as-48 addition on the bacterial communities of cherimoya the present study, pulp obtained from cherimoya pulp (annona cherimola) was inoculated with epiphytic microbiota collected from cherimoya fruits, and supplemented or not with the circular bacteriocin enterocin as-48 (50μg/g) and then packed under vacuum. samples supplemented or not with enterocin were treated by high hydrostatic pressure (600mpa, 8min) and then stored at 5°c for 30days. the single as-48 treatment only delayed microbial growth non-significantly (p>0.05). hhp treatment reduced ...201525528726
isolation and identification of biocellulose-producing bacterial strains from malaysian acidic fruits.biocellulose (bc) is pure extracellular cellulose produced by several species of micro-organisms that has numerous applications in the food, biomedical and paper industries. however, the existing biocellulose-producing bacterial strain with high yield was limited. the aim of this study was to isolate and identify the potential biocellulose-producing bacterial isolates from malaysian acidic fruits. one hundred and ninety-three bacterial isolates were obtained from 19 local acidic fruits collected ...201627002476
functional and photochemical characterization of a light-driven proton pump from the gammaproteobacterium pantoea vagans.photoactive retinal proteins are widely distributed throughout the domains of the microbial world (i.e., bacteria, archaea, and eukarya). here we describe three retinal proteins belonging to a phylogenetic clade with a unique dtg motif. light-induced decrease in the environmental ph and its inhibition by carbonyl cyanide m-chlorophenylhydrazone revealed that these retinal proteins function as light-driven outward electrogenic proton pumps. we further characterized one of these proteins, pantoea ...201626970049
[the novel intracellular phytase of enterobacteriaceae: isolation and properties].for the first time phytase enzyme was isolated from pantoea vagans 3.2 strain and was subjected to investigation. the enzyme was purified about 474-fold to apparent homogeneity from the crude extract of the strain, its primary structure was determined and it was concluded that phytase of pantoea vagans 3.2 belongs to the family of histidine acid phosphatases. it has a molecular mass of about 46 kda and km for the hydrolysis of sodium phytate was 0.28 mm. some physicochemical properties ofphytase ...201424707723
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