| temperature-dependent wsm1 and wsm2 gene-specific blockage of viral long-distance transport provides resistance to wheat streak mosaic virus and triticum mosaic virus in wheat. | wheat streak mosaic virus (wsmv) and triticum mosaic virus (trimv) are economically important viral pathogens of wheat. wheat cvs. mace, carrying the wsm1 gene, is resistant to wsmv and trimv, and snowmass, with wsm2, is resistant to wsmv. viral resistance in both cultivars is temperature sensitive and is effective at 18°c or below but not at higher temperatures. the underlying mechanisms of viral resistance of wsm1 and wsm2, nonallelic single dominant genes, are not known. in this study, we fou ... | 2016 | 27551888 |
| population dynamics of the wheat curl mite (acari: eriophyidae) during the heading stages of winter wheat. | the wheat curl mite (aceria tosichella keifer) is the only known vector of three viruses in wheat-wheat streak mosaic virus, wheat mosaic virus, and triticum mosaic virus. the economic impact of this disease complex is linked to the presence of suitable hosts prior to winter wheat maturing in early summer and the movement of wheat curl mite from wheat to oversummering hosts prior to wheat harvest. previous research has documented the prevalence and density of mite populations on maturing wheat h ... | 2017 | 28334133 |
| immunodetection of triticum mosaic virus by das- and dac-elisa using antibodies produced against coat protein expressed in escherichia coli: potential for high-throughput diagnostic methods. | triticum mosaic virus (trimv), an economically important virus infecting wheat in the great plains region of the usa, is the type species of the poacevirus genus in the family potyviridae. sensitive and high-throughput serology-based detection methods are crucial for the management of trimv and germplasm screening in wheat breeding programs. in this study, trimv coat protein (cp) was expressed in escherichia coli, and polyclonal antibodies were generated against purified soluble native form reco ... | 2013 | 23454646 |
| multiple categories of resistance to wheat curl mite (acari: eriophyidae) expressed in accessions of aegilops tauschii. | the wheat curl mite, aceria tosichella keifer, is an important pest in the western plains of the united states as well as in most major wheat-growing regions of the world. this mite is a vector of the economically important diseases wheat streak mosaic virus (wsmv), triticum mosaic virus (trimv), and high plains virus (hpv). this study looked at seven accessions of aegilops tauschii (coss) schmal to determine if they exhibit antibiosis, tolerance, and/or antixenosis to the wheat curl mite using ... | 2012 | 23356085 |
| Impact of Triticum mosaic virus infection on hard winter wheat milling and bread baking quality. | BACKGROUND: Triticum mosaic virus (TriMV) is a newly discovered wheat virus. Information regarding the effect of wheat viruses on milling and baking quality is limited. The objective of this study was to determine the impact of TriMV infection on the kernel characteristics, milling yield and bread baking quality of wheat. Commercial hard winter varieties evaluated included RonL, Danby and Jagalene. The TriMV resistance of RonL is low, while that of Danby and Jagalene is unknown. KS96HW10-3, a ge ... | 2011 | 21969097 |
| complete nucleotide sequence and taxonomy of sugarcane streak mosaic virus, member of a novel genus in the family potyviridae. | the complete genomic sequence of a pakistani isolate of sugarcane streak mosaic virus (scsmv-pak) is determined to be 9782 nucleotides in length, excluding the 3' poly(a) tail, and it comprises a large open reading frame encoding a polyprotein of 3130 amino acid residues. the deduced polyprotein is likely to be cleaved at nine putative protease sites by three viral proteases to ten mature proteins. conserved motifs of orthologous proteins of other potyviruses are identified in corresponding posi ... | 2010 | 20162446 |
| multiplex real-time rt-pcr for detection of wheat streak mosaic virus and triticum mosaic virus. | wheat streak mosaic virus (wsmv) and triticum mosaic virus (trimv) are widespread throughout the southwestern great plains states. when using conventional diagnostics such as enzyme-linked immunosorbent assays (elisa), these two viruses are commonly found together in infected wheat samples. methods for molecular detection have been developed for wheat viral pathogens, but until recently no multiplex method for detection of both wsmv and trimv within a single sample was available. therefore, the ... | 2010 | 20138086 |
| wheat cultivar-specific disease synergism and alteration of virus accumulation during co-infection with wheat streak mosaic virus and triticum mosaic virus. | triticum mosaic virus (trimv), the type member of the newly proposed poacevirus genus, and wheat streak mosaic virus (wsmv), the type member of tritimovirus genus of the family potyviridae, infect wheat naturally in the great plains and are transmitted by wheat curl mites. in this study, we examined the ability of these viruses to infect selected cereal hosts, and found several differential hosts between trimv and wsmv. additionally, we examined the interaction between wsmv and trimv in three wh ... | 2010 | 20128696 |
| the complete genome sequence of triticum mosaic virus, a new wheat-infecting virus of the high plains. | the genome of triticum mosaic virus (trimv), a recently discovered mite-transmitted wheat potyvirus, was sequenced, characterized, and compared to other members of the family potyviridae. trimv has a single mrna strand of 10,266 nucleotides with a predicted polyprotein consisting of 3,112 peptides. protein alignments of the coat protein demonstrate that trimv has 45.9% identity to sugarcane streak mosaic virus strain ap (scsmv-ap), but shares only 23.2% identity to wheat streak mosaic virus. alt ... | 2009 | 19649764 |
| triticum mosaic virus: a distinct member of the family potyviridae with an unusually long leader sequence. | the complete genome sequence of triticum mosaic virus (trimv), a member in the family potyviridae, has been determined to be 10,266 nucleotides (nt) excluding the 3' polyadenylated tail. the genome encodes a large polyprotein of 3,112 amino acids with the "hall-mark proteins" of potyviruses, including a small overlapping gene, pipo, in the p3 cistron. the genome of trimv has an unusually long 5' nontranslated region of 739 nt with 12 translation initiation codons and three small open reading fra ... | 2009 | 19594313 |
| triticum mosaic poacevirus enlists p1 rather than hc-pro to suppress rna silencing-mediated host defense. | triticum mosaic virus (trimv) is the type species of the newly established poacevirus genus in the family potyviridae. in this study, we demonstrate that in contrast to the helper component-proteinase (hc-pro) of potyvirus species, the p1 proteins of trimv and sugarcane streak mosaic poacevirus function in suppression of rna silencing (srs). trimv p1 effectively suppressed silencing induced by single- or double-stranded rnas (ss/ds rnas), and disrupted the systemic spread of silencing signals at ... | 2012 | 22877841 |
| the role of the 5' untranslated regions of potyviridae in translation. | the potyviridae family relies on a cap-independent translation mechanism to facilitate protein expression. the genomic architecture of the viral rnas of the potyviridae family resembles those of the animal picornaviruses. the viral genomes lack a 5' cap structure. instead, they have the viral protein vpg covalently linked to the 5' end of the rna. the viral rnas code for a single large polyprotein, which is then cleaved into several functional subunits. with their common genome organization with ... | 2015 | 25683508 |
| a unique 5' translation element discovered in triticum mosaic virus. | several plant viruses encode elements at the 5' end of their rnas, which, unlike most cellular mrnas, can initiate translation in the absence of a 5' m7gpppg cap. here, we describe an exceptionally long (739-nucleotide [nt]) leader sequence in triticum mosaic virus (trimv), a recently emerged wheat pathogen that belongs to the potyviridae family of positive-strand rna viruses. we demonstrate that the trimv 5' leader drives strong cap-independent translation in both wheat germ extract and oat pro ... | 2015 | 26423954 |
| the triticum mosaic virus 5' leader binds to both eif4g and eifiso4g for translation. | we recently identified a remarkably strong (739 nt-long) ires-like element in the 5' untranslated region (utr) of triticum mosaic virus (trimv, potyviridae). here, we define the components of the cap-binding translation initiation complex that are required for trimv translation. using bio-layer interferometry and affinity capture of the native translation apparatus, we reveal that the viral translation element has a ten-fold greater affinity for the large subunit eif4g/eifiso4g than to the cap b ... | 2017 | 28046134 |
| genetic characterization of north american populations of the wheat curl mite and dry bulb mite. | the wheat curl mite, aceria tosichella keifer, transmits at least three harmful viruses, wheat streak mosaic virus (wsmv), high plains virus (hpv), and triticum mosaic virus (trimv) to wheat (triticum aestivum l.) throughout the great plains. this virus complex is considered to be the most serious disease of winter wheat in the western great plains. one component of managing this disease has been developing mite resistance in wheat; however, identification of mite biotypes has complicated deploy ... | 2012 | 23156180 |
| a multi-target, non-infectious and clonable artificial positive control for routine pcr-based assays. | positive controls are essential for pcr reliability and are challenging to obtain for rare, exotic and/or emerging pathogens and pose biosafety risks if manufactured using infectious pathogens. custom synthetic dna inserts can be designed de novo in tandems of forward and reverse complement priming sequences to be inserted in circular plasmid vectors. to test this concept, artificial positive controls (apcs) for use in pcr were synthesized to contain primer sequences targeting four viruses (barl ... | 2013 | 24013035 |
| dynamics of small rna profiles of virus and host origin in wheat cultivars synergistically infected by wheat streak mosaic virus and triticum mosaic virus: virus infection caused a drastic shift in the endogenous small rna profile. | co-infection of wheat (triticum aestivum l.) by wheat streak mosaic virus (wsmv, a tritimovirus) and triticum mosaic virus (trimv, a poacevirus) of the family potyviridae causes synergistic interaction. in this study, the effects of the synergistic interaction between wsmv and trimv on endogenous and virus-derived small interfering rnas (vsirnas) were examined in susceptible ('arapahoe') and temperature-sensitive resistant ('mace') wheat cultivars at 18°c and 27°c. single and double infections i ... | 2014 | 25365307 |
| in vitro transcripts of wild-type and fluorescent protein-tagged triticum mosaic virus (family potyviridae) are biologically active in wheat. | triticum mosaic virus (trimv) (genus poacevirus, family potyviridae) is a recently described eriophyid mite-transmitted wheat virus. in vitro rna transcripts generated from full-length cdna clones of trimv proved infectious on wheat. wheat seedlings inoculated with in vitro transcripts elicited mosaic and mottling symptoms similar to the wild-type virus, and the progeny virus was efficiently transmitted by wheat curl mites, indicating that the cloned virus retained pathogenicity, movement, and w ... | 2015 | 26214124 |
| the effect of temperature, relative humidity, and virus infection status on off-host survival of the wheat curl mite (acari: eriophyidae). | the wheat curl mite, aceria tosichella keifer, is an eriophyid pest of wheat, although its primary economic impact on wheat is due to the transmission of wheat streak mosaic (wsmv), wheat mosaic (also known as high plains virus), and triticum mosaic (trimv) viruses. these viruses cause significant annual losses in winter wheat production throughout the western great plains. temperature and humidity are factors that often influence arthropod survival, especially during dispersal from their hosts, ... | 2015 | 26470294 |
| triticum mosaic virus exhibits limited population variation yet shows evidence of parallel evolution after replicated serial passage in wheat. | an infectious cdna clone of triticum mosaic virus (trimv) (genus poacevirus; family potyviridae) was used to establish three independent lineages in wheat to examine intra-host population diversity levels within protein 1 (p1) and coat protein (cp) cistrons over time. genetic variation was assessed at passages 9, 18 and 24 by single-strand conformation polymorphism, followed by nucleotide sequencing. the founding p1 region genotype was retained at high frequencies in most lineage/passage populat ... | 2016 | 26914507 |
| the coat protein and nia protease of two potyviridae family members independently confer superinfection exclusion. | superinfection exclusion (sie) is an antagonistic virus-virus interaction whereby initial infection by one virus prevents subsequent infection by closely related viruses. although sie has been described in diverse viruses infecting plants, humans, and animals, its mechanisms, including involvement of specific viral determinants, are just beginning to be elucidated. in this study, sie determinants encoded by two economically important wheat viruses, wheat streak mosaic virus (wsmv; genus tritimov ... | 2016 | 27681136 |
| homoeologous recombination-based transfer and molecular cytogenetic mapping of a wheat streak mosaic virus and triticum mosaic virus resistance gene wsm3 from thinopyrum intermedium to wheat. | here, we report the production of a wheat- thinopyrum intermedium recombinant stock conferring resistance to wheat streak mosaic virus and triticum mosaic virus. wheat streak mosaic caused by the wheat streak mosaic virus (wsmv) is an important disease of bread wheat (triticum aestivum) worldwide. to date, only three genes conferring resistance to wsmv have been named and two, wsm1 and wsm3, were derived from the distantly related wild relative thinopyrum intermedium. wsm3 is only available in t ... | 2017 | 27900400 |
| wheat genotypes with combined resistance to wheat curl mite, wheat streak mosaic virus, wheat mosaic virus, and triticum mosaic virus. | the wheat curl mite, aceria tosichella keifer, (wcm) is a global pest of bread wheat that reduces yields significantly. in addition, wcm carries wheat streak mosaic virus (wsmv, family potyviridae, genus tritimovirus), the most significant wheat virus in north america; high plains wheat mosaic virus (hpwmov, genus emaravirus, formerly high plains virus); and triticum mosaic virus (trimv, family potyviridae, genus poacevirus). viruses carried by wcm have reduced wheat yields throughout the u.s. g ... | 2017 | 28087646 |